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Biosensors & Bioelectronics

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https://www.readbyqxmd.com/read/29351868/an-aptamer-nanopore-enabled-microsensor-for-detection-of-theophylline
#1
Silu Feng, Changtian Chen, Wei Wang, Long Que
This paper reports an aptamer-based nanopore thin film sensor for detecting theophylline in the buffer solution and complex fluids including plant extracts and serum samples. Compared to antibody-based detection, aptamer-based detection offers many advantages such as low cost and high stability at elevated temperatures. Experiments found that this type of sensor can readily detect theophylline at a concentration as low as 0.05µM, which is much lower than the detection limit of current lab-based equipment such as liquid chromatography (LC)...
January 10, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29351869/allosteric-kissing-complex-based-electrochemical-biosensor-for-sensitive-regenerative-and-versatile-detection-of-proteins
#2
Mingsha Zhao, Shanshan Zhang, Zhiqiang Chen, Changzhi Zhao, Li Wang, Shufeng Liu
Herein, an allosteric kissing complex-based electrochemical biosensor was ingeniously proposed for the simple, sensitive, regenerative and versatile detection of proteins. Two hairpins (Hp1 and Hp2) were designed and the Hp1 was immobilized on the electrode surface, which could form a kissing complex with Hp2 through the apical loop-loop or kissing interaction of the RNA-RNA base sequences. The Hp2 possesses the appended single-stranded tails on each end, which hybridize with the recognition element-conjugated DNA strands to construct a protein responsive switch of Hp2 scaffold...
January 9, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29351867/electrosynthesized-mips-for-transferrin-plastibodies-or-nano-filters
#3
Xiaorong Zhang, Aysu Yarman, Júlia Erdossy, Sagie Katz, Ingo Zebger, Katharina J Jetzschmann, Zeynep Altintas, Ulla Wollenberger, Róbert E Gyurcsányi, Frieder W Scheller
Molecularly imprinted polymer (MIP) nanofilms for transferrin (Trf) have been synthesized on gold surfaces by electro-polymerizing the functional monomer scopoletin in the presence of the protein target or around pre-adsorbed Trf. As determined by atomic force microscopy (AFM) the film thickness was comparable with the molecular dimension of the target. The target (re)binding properties of the electro-synthesized MIP films was evaluated by cyclic voltammetry (CV) and square wave voltammetry (SWV) through the target-binding induced permeability changes of the MIP nanofilms to the ferricyanide redox marker, as well as by surface plasmon resonance (SPR) and surface enhanced infrared absorption spectroscopy (SEIRAS) of the immobilized protein molecules...
January 9, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29346077/three-dimensional-graphene-biointerface-with-extremely-high-sensitivity-to-single-cancer-cell-monitoring
#4
Xiahua Wang, Aiping Liu, Yun Xing, Hongwei Duan, Weizhong Xu, Qi Zhou, Huaping Wu, Cen Chen, Benyong Chen
We developed a three-dimensional biointerface of graphene-based electrical impedance sensor for metastatic cancer diagnosis at single-cell resolution. Compared with traditional impedance sensor with two-dimensional interface, the graphene biointerface mimiced the topography and somatotype features of cancer cells, achieving more comprehensive and thorough single cell signals in the three-dimensional space. At the nodes of physiological behavior change of single cell, namely cell capture, adhesion, migration and proliferation, the collected electrical signals from graphene biointerface were about two times stronger than those from the two-dimensional gold interface due to the substantial increase in contact area and significant improvement of topographical interaction between cells and graphene electrode...
January 9, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29346076/ultrasensitive-electrochemical-immuno-sensing-platform-based-on-gold-nanoparticles-triggering-chlorpyrifos-detection-in-fruits-and-vegetables
#5
Anita Talan, Annu Mishra, Sergei A Eremin, Jagriti Narang, Ashok Kumar, Sonu Gandhi
Chlorpyrifos (chl) is an organophosphate pesticide extensively used in agriculture and highly toxic for human health. Fluorine doped tin-oxide (FTO) based electrochemical nanosensor was developed for chlorpyrifos detection with gold nanoparticles (AuNPs) and anti-chlorpyrifos antibodies (chl-Ab). AuNPs provides high electrical conductivity and specific resistivity, thus increases the sensitivity of immunoassay. High electrical conductivity of AuNPs reveals that it promotes the redox reaction for better cyclic voltammetry...
January 9, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29331900/a-catalytic-and-dual-recycling-amplification-atp-sensor-based-on-target-driven-allosteric-structure-switching-of-aptamer-beacons
#6
Ying Peng, Daxiu Li, Ruo Yuan, Yun Xiang
Abnormal concentrations of ATP are associated with many diseases and cancers, and quantitative detection of ATP is thus of great importance for disease diagnosis and prognosis. In the present work, we report a new dual recycling amplification sensor integrated with catalytic hairpin assembly (CHA) to achieve high sensitivity for fluorescent detection of ATP. The association of the target ATP with the aptamer beacons causes the allosteric structure switching of the aptamer beacons to expose the toehold regions, which hybridize with and unfold the fluorescently quenched hairpin signal probes (HP1) to recycle the target ATP and to trigger CHA between HP1 and the secondary hairpin probes (HP2) to form HP1/HP2 duplexes...
January 9, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29331901/an-ultrasensitive-detection-of-mirna-155-in-breast-cancer-via-direct-hybridization-assay-using-two-dimensional-molybdenum-disulfide-field-effect-transistor-biosensor
#7
Samira Mansouri Majd, Abdollah Salimi, Foad Ghasemi
MicroRNAs (miRNAs), critical biomarkers of acute and chronic diseases, play key regulatory roles in many biological processes. As a result, robust assay platforms to enable an accurate and efficient detection of low-level miRNAs in complex biological samples are of great significance. In this work, a label-free and direct hybridization assay using molybdenum disulfide (MoS2) field-effect transistor (FET) biosensor has been developed for ultrasensitive detection of miRNA-155 as a breast cancer biomarker in human serum and cell-line samples...
January 6, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29331429/paper-based-fluorescent-sensor-via-aggregation-induced-emission-fluorogen-for-facile-and-sensitive-visual-detection-of-hydrogen-peroxide-and-glucose
#8
Jiafu Chang, Haiyin Li, Ting Hou, Wenna Duan, Feng Li
Hydrogen peroxide (H2O2), an important reactive oxygen species (ROS), is related to the oxidative stress in organisms, and plays important roles in a variety of cellular activities as well. So it is of crucial importance to develop sensitive and accurate sensing strategies to detect H2O2 in biological systems. Herein, by taking advantage of the unique emission characteristics of aggregation induced emission (AIE) fluorogens, we proposed a non-enzymatic fluorescence platform for facile and sensitive detection of H2O2, both in solution state using fluorescence spectrometer and on paper-based sensor via visual inspection...
January 6, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29331427/water-soluble-mercury-ion-sensing-based-on-the-thymine-hg2-thymine-base-pair-using-retroreflective-janus-particle-as-an-optical-signaling-probe
#9
Hyeong Jin Chun, Saemi Kim, Yong Duk Han, Dong Woo Kim, Ka Ram Kim, Hyo-Sop Kim, Jae-Ho Kim, Hyun C Yoon
Herein, we report an optical sensing platform for mercury ions (Hg2+) in water based on the integration of Hg2+-mediated thymine-thymine (T-T) stabilization, a biotinylated stem-loop DNA probe, and a streptavidin-modified retroreflective Janus particle (SA-RJP). Two oligonucleotide probes, including a stem-loop DNA probe and an assistant DNA probe, were utilized. In the absence of Hg2+, the assistant DNA probe does not hybridize with the stem-loop probe due to their T-T mismatch, so the surface-immobilized stem-loop DNA probe remains a closed hairpin structure...
January 6, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29331428/heating-enhanced-sensitive-and-selective-electrochemical-detection-of-hg2-based-on-t-hg2-t-structure-and-exonuclease-iii-assisted-target-recycling-amplification-strategy-at-heated-gold-disk-electrode
#10
Shao-Hua Wu, Biao Zhang, Fang-Fang Wang, Zhen-Zhen Mi, Jian-Jun Sun
A sensitive and selective electrochemical Hg2+ sensor was developed based on T-Hg2+-T structure and exonuclease (Exo) III -assisted target recycling amplification at heated gold disk electrode (HAuDE). First, a DNA signal probe P1 was for the first time designed and labeled with ferrocene (Fc) near the attached SH-5'-end, so as to shorten the distance between Fc and the electrode and enhance the initial current of Fc compared with that labeled at the 3'-end far from the electrode. Then the signal amplification was achieved by Exo III-assisted Hg2+ recycling...
January 5, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29331426/hybrid-porous-thin-films-opportunities-and-challenges-for-sensing-applications
#11
REVIEW
Pawan Kumar, Ki-Hyun Kim, Kowsalya Vellingiri, Pallabi Samaddar, Parveen Kumar, Akash Deep, Naresh Kumar
In this paper, the scientific progress in the field of thin film materials and their associated sensing technologies are described comprehensively to address the directions for future research and developments as per the need of modern-day technologies. To begin with, we briefly discussed the fundamental synthesis approaches for advanced thin films with an emphasis on the properties necessary for controlled fabrication (e.g., the elemental ratio and spatial arrangement). Subsequently, we explored the control, characterization, and optimization of hybrid porous thin films with respect to diverse sensing applications...
January 5, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29328971/ultrasensitive-electrochemical-sensing-of-hg2-based-on-thymine-hg2-thymine-interaction-and-signal-amplification-of-alkaline-phosphatase-catalyzed-silver-deposition
#12
Aigui Xu, Long Chao, Hongbo Xiao, Yuyun Sui, Jia Liu, Qingji Xie, Shouzhuo Yao
We report an ultrasensitive electrochemical sensor for Hg2+ detection, on the basis of two Hg2+-specific oligonucleotide probes (a thiolated capture probe and a biotinated signal probe), "terminal" signal amplification of alkaline phosphatase catalyzed deposition of silver and in situ microliter-droplet anodic stripping voltammetry (ASV). The thiolated capture probe is immobilized on an Au-plated glassy carbon electrode, and the biotinated signal probe is then attached to the electrode surface through the thymine-Hg2+-thymine interaction in the presence of Hg2+...
January 5, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29331430/disposable-inkjet-printed-electrochemical-platform-for-detection-of-clinically-relevant-her-2-breast-cancer-biomarker
#13
Susanita Carvajal, Samantha N Fera, Abby L Jones, Thaisa A Baldo, Islam M Mosa, James F Rusling, Colleen E Krause
Rapidly fabricated, disposable sensor platforms hold tremendous promise for point-of-care detection. Here, we present an inexpensive (< $0.25) fully inkjet printed electrochemical sensor with integrated counter, reference, and working electrodes that is easily scalable for commercial fabrication. The electrochemical sensor platform featured an inkjet printed gold working 8-electrode array (WEA) and counter electrode (CE), along with an inkjet -printed silver electrode that was chlorinated with bleach to produce a Ag/AgCl quasi-reference electrode (RE)...
January 4, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29328969/poly-3-6-diamino-9-ethylcarbazole-based-molecularly-imprinted-polymer-sensor-for-ultra-sensitive-and-selective-detection-of-17-%C3%AE-estradiol-in-biological-fluids
#14
Weilu Liu, Haifeng Li, Shangmin Yu, Jiaxing Zhang, Weihua Zheng, Liting Niu, Gengen Li
In this work, we reported the synthesis of 3, 6-diamino-9-ethylcarbazole and its application as a new monomer for preparation of molecularly imprinted polymer (MIP) electrochemical sensor. The as prepared MIP sensor exhibited ultrahigh sensitivity and selectivity for the detection of 17-β-estradiol in attomolar levels (1 × 10-18molL-1). The sensor works by detecting the change of the interfacial impedance that is derived from recognition of 17-β-estradiol on the MIP layer. The MIP sensor based on 3, 6-diamino-9-ethylcarbazole monomer revealed better performance than that of unmodified carbazole monomer...
January 4, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29324284/a-novel-cytosensor-based-on-pt-ag-nanoflowers-and-aunps-acetylene-black-for-ultrasensitive-and-highly-specific-detection-of-circulating-tumor-cells
#15
Sitian Tang, Huawei Shen, Yixiong Hao, Zhenglan Huang, Yiyi Tao, Yang Peng, Yongcan Guo, Guoming Xie, Wenli Feng
Circulating tumor cells (CTCs), as the cellular origin of metastasis, are cancer cells that break away from a primary tumor and circulate in the peripheral blood. And they provide a wealth of information about tumor phenotype. Here, this work reported a novel ultrasensitive immunoassay protocol for the detection of CTCs by using Pt@Ag nanoflowers (Pt@AgNFs) and AuNPs/Acetylene black (AuNPs/AB) nanomaterial. In the established approach, AuNPs/AB nanomaterial was used as substrate material to increase the specific surface area and enhance the conductivity of the gold electrode...
January 4, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29306029/proximity-ligation-detection-of-lectin-concanavalin-a-and-fluorescence-imaging-cancer-cells-using-carbohydrate-functionalized-dna-silver-nanocluster-probes
#16
Xiaoru Zhang, Fengting Chen, Xiaojie Song, Peng He, Shusheng Zhang
A novel method for study the interaction between carbohydrate and lectin was developed in homogenous solution. DNA was introduced to the recognition process of carbohydrate with protein. Due to the versatile detection mode of DNA, the detection efficiency for carbohydrate and protein was highly promoted. Herein, two kinds of mannose-DNA conjugates were synthesized. One of which was used to prepare DNA-templated silver nanocluster (AgNCs) and the other one contained guanine-rich DNA sequences. When the mannose of two conjugates binding to lectin Concanavalin A (Con A), great enhancement on fluorescence intensity was obtained due to the proximity ligation of the DNA-templated Ag NCs with guanine-rich DNA sequences...
December 31, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29306030/exonuclease-iii-boosted-cascade-reactions-for-ultrasensitive-sers-detection-of-nucleic-acids
#17
Yudie Sun, Pai Peng, Ruiyan Guo, Huihui Wang, Tao Li
A variety of nucleic acid amplification techniques have been integrated into different detection methods to promote the development of sensitive and convenient analysis of nucleic acids. However, it is still in urgent need to develop amplified nucleic acid biosensors for the analysis of susceptible gene and even distinguishing single-base mismatched DNA in complex biological samples. Benefiting from the achieved detection strategies, here we boost isothermal nucleic acid amplification by resorting to enzyme amplification, and combine this two-stage amplification method with surface-enhanced Raman spectroscopy (SERS) to develop a signal-on nucleic acid detection platform...
December 29, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29328970/spore-based-biosensor-to-monitor-the-microbicidal-efficacy-of-gaseous-hydrogen-peroxide-sterilization-processes
#18
Jan Oberländer, Marlena Mayer, Anton Greeff, Michael Keusgen, Michael J Schöning
In this work, a spore-based biosensor is evaluated to monitor the microbicidal efficacy of sterilization processes applying gaseous hydrogen peroxide (H2O2). The sensor is based on interdigitated electrode structures (IDEs) that have been fabricated by means of thin-film technologies. Impedimetric measurements are applied to study the effect of sterilization process on spores of Bacillus atrophaeus. This resilient microorganism is commonly used in industry to proof the sterilization efficiency. The sensor measurements are accompanied by conventional microbiological challenge tests, as well as morphological characterizations with scanning electron microscopy (SEM) and transmission electron microscopy (TEM)...
December 27, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29324283/a-novel-electrochemiluminescent-biosensor-based-on-resonance-energy-transfer-between-poly-9-9-di-n-octylfluorenyl-2-7-diyl-and-3-4-9-10-perylenetetracar-boxylic-acid-for-insulin-detection
#19
Han Zhang, Fumei Zuo, Xingrong Tan, Shenghao Xu, Ruo Yuan, Shihong Chen
An electrochemiluminescencent (ECL) biosensor was designed for the determination of insulin using a novel ECL resonance energy transfer (ECL-RET) strategy. In this strategy, carboxyl poly(9,9-dioctyfluorenyl-2,7-diyl) dots (PFO dots) were worked as ECL donor and 3,4,9,10-perylenetetracar-boxylic acid (PTCA) exploited as ECL acceptor, and hydrogen peroxide (H2O2) employed as the coreactant. The ECL donor and ECL acceptor were separately labeled with primary antibody (Ab1) and secondary antibody (Ab2), forming a sensing interface to the analyte target, insulin...
December 27, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29306762/microfluidic-platform-for-single-cell-analysis-under-dynamic-spatial-and-temporal-stimulation
#20
Jiyoung Song, Hyunryul Ryu, Minhwan Chung, Youngtaek Kim, Yannick Blum, Sung Sik Lee, Olivier Pertz, Noo Li Jeon
Recent research on cellular responses is shifting from static observations recorded under static stimuli to real-time monitoring in a dynamic environment. Since cells sense and interact with their surrounding microenvironment, an experimental platform where dynamically changing cellular microenvironments should be recreated in vitro. There has been a lack of microfluidic devices to support spatial and temporal stimulations in a simple and robust manner. Here, we describe a microfluidic device that generates dynamic chemical gradients and pulses in both space and time using a single device...
December 27, 2017: Biosensors & Bioelectronics
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