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Biosensors & Bioelectronics

Muhammet Aydın, Elif Burcu Aydın, Mustafa Kemal Sezgintürk
A novel label-free impedimetric immunosensor was fabricated for rapid, selective and sensitive detection and quantification of Cadherin-like protein 22 (CDH22), a cancer marker, in human serum by using easy and quickly prepared disposable ITO immunoelectrode. cancer marker, in human serum by using easy and quickly prepared disposable ITO immunoelectrode. The biosensing approach implied the use of ITO electrode coated with poly(phosphazene) polymer including benzaldehyde groups attached with CDH22 antibody and CDH22 antigens...
November 2, 2018: Biosensors & Bioelectronics
Xianzhen Song, Xiaojian Li, Dong Wei, Rui Feng, Tao Yan, Yaoguang Wang, Xiang Ren, Bin Du, Hongmin Ma, Qin Wei
In this work, 3,4,9,10-perylenetetracar-boxylic acid (PTCA) as luminophor was grafted on the surface of graphene oxide (PTCA-GO) directly. GO exhibited large specific surface area and excellent electrical conductivity which can immobilize large amounts of PTCA to improve the electrochemiluminescence (ECL) efficiency. Moreover, gold nanoparticles (Au NPs) were anchored on the surface of PTCA-GO to immobilize primary antibodies (Ab1 ) via Au-NH2 bond and enhance the electron transport of PTCA-GO. CuS was used as a novel co-reaction accelerator in PTCA-K2 S2 O8 system to label secondary antibodies (Ab2 ), which can react with the coreactant (K2 S2 O8 ) to produce more SO4 •- ...
November 2, 2018: Biosensors & Bioelectronics
Dominika Kalinowska, Ilona Grabowska-Jadach, Monika Liwinska, Marcin Drozd, Mariusz Pietrzak, Artur Dybko, Zbigniew Brzozka
Herein, we present the research focused on the synthesis and application of aptamer-modified gold nanoshells for photothermal therapy (PTT). NIR-absorbing hollow gold nanoshells were synthetized and conjugated with anti-MUC1 aptamer (HGNs@anti-MUC1). MUC1 (Mucin 1) is a transmembrane glycoprotein, which is overexpressed in a variety of epithelial cancers (eg. breast, lung, pancreatic). In order to evaluate the efficiency of PTT with HGNs@anti-MUC1 we used 3D cell culture model - multicellular spheroids. The selected cell culture model is considered as the best in vitro model for cancer research (similar morphology, metabolite and oxygen gradients, cellular interactions and cell growth kinetics in the spheroids are similar to the early stage of a nonvascular tumor)...
November 2, 2018: Biosensors & Bioelectronics
SeJin Kim, Dami Kim, Sanghyo Kim
The research progress in the centrifugal microfluidic platform provides great opportunities for simple and effective analytical measurements in a variety of areas including biomedical engineering. In this study, we propose an optical reader that can measure the transmittance in a very sensitive and rapid manner on a hybrid paper-polymer centrifugal disc platform. This device enables real-time monitoring of multiple samples by measuring the absorbance of the light transmitted through the paper integrated on the disc between the light emitting diode (LED) and the photodiode (PD) regardless of the ambient light condition...
October 31, 2018: Biosensors & Bioelectronics
Yao Chen, Pei-Xin Yuan, Ai-Jun Wang, Xiliang Luo, Yadong Xue, Lu Zhang, Jiu-Ju Feng
Herein, a novel electrochemical ultrasensitive immunosensor was designed for detecting prostate-specific antigen (PSA) with three-dimensional (3D) PtCu hollow nanoframes (PtCu HNFs) as signal amplification. The highly opened PtCu HNFs were synthesized by a one-pot solvothermal method with cetyltrimethylammonium chloride (CTAC) and trishydroxymethyl aminomethane (Tris) as co-structuring directors. The architectures enlarged the loading of prostate specific antibodies (Ab) and efficiently catalyzed hydrogen peroxide (H2 O2 ) reaction, ultimately amplifying the signals...
October 29, 2018: Biosensors & Bioelectronics
Muhammad Adeel, Md Mahbubur Rahman, Jae-Joon Lee
A novel electrochemical aptasensor based on gold nanoparticles decorated on boron nitride nanosheets (AuNPs/BNNSs) for the sensitive and selective detection of myoglobin (Mb) is reported. BNNSs were chemically synthesized by a low-cost and simple hydrothermal method. They were deposited onto the fluorine-doped tin oxide (FTO) electrode by a spin-coating method. Subsequently, AuNPs were chemically deposited onto the BNNS/FTO electrode by a seed-mediated chemical reduction method, with an average particle size of approximately 10 nm...
October 29, 2018: Biosensors & Bioelectronics
Fangfang Wang, Cuiling Zhang, Xuetong Qu, Shasha Cheng, Yuezhong Xian
Elevated hydrogen sulfide (H2 S) level is closely associated with various diseases. So the sensing of H2 S is noteworthy for divulging its role in diagnosing these diseases. Herein, we proposed poly(acrylic acid)-modified upconversion nanoparticles assembled with cationic near-infrared cyanine chromophores (Cy7-Cl) as the nanoprobe (Cy7-UCNPs) for monitoring H2 S based on thiolation reactions. The presence of H2 S resulted into about five-fold enhancement in the luminescence intensity of Cy7-UCNPs and the nanoprobe showed a good linearity (R2 =0...
October 27, 2018: Biosensors & Bioelectronics
Jeffrey G Bell, Maral P S Mousavi, Mohamed K Abd El-Rahman, Edward K W Tan, Shervanthi Homer-Vanniasinkam, George M Whitesides
Bilirubin is predominantly formed in the liver as a result of breakdown of hemoglobin. Knowing the concentration of bilirubin in serum is important in evaluating the health of the liver, and for the diagnosis of hyperbilirubinemia (a condition that afflicts approximately 60% of full-term and 80% of pre-term newborns). This paper describes the design and fabrication of a potentiometric sensor for the determination of free bilirubin in serum. The sensor has a polymeric ion-selective membrane, and selectively measures free ionic bilirubin ("unbound" bilirubin - i...
October 27, 2018: Biosensors & Bioelectronics
Raji Atchudan, Nallal Muthuchamy, Thomas Nesakumar Jebakumar Immanuel Edison, Suguna Perumal, Rajangam Vinodh, Kang Hyun Park, Yong Rok Lee
In this work, an ultra-sensing photoelectrochemical (PEC) glucose biosensor has been constructed from the bio-derived nitrogen-doped carbon sheets (NDC) wrapped titanium dioxide nanoparticles (NDC-TiO2 NPs) followed by the covalent immobilization of glucose oxidase (GODx) on them (designated as a GODx/NDC-TiO2 NPs/ITO biosensor). Initially, the TiO2 NPs was synthesized by sol-gel method and then NDC-TiO2 NPs was synthesized utilizing a green source of Prunus persica (peach fruit) through a simple hydrothermal process...
October 26, 2018: Biosensors & Bioelectronics
Zhuang Liu, Yachao Zhang, Congcong Bian, Tian Xia, Yan Gao, Xueli Zhang, Huimin Wang, Hanyue Ma, Yuan Hu, Xia Wang
A highly sensitive whole cell based electrochemical biosensor was developed for catechol detection in this study. The carE gene of Sphingobium yanoikuyae XLDN2-5 encoding catechol 2,3-dioxygenase (C23O), a key enzyme in the biodegradation of aromatic compound, was cloned and over-expressed in Escherichia coli BL21 (E. coli BL21). Compared to Sphingobium yanoikuyae XLDN2-5, the recombinant E. coli BL21 over-expressed C23O exhibited higher catalytic activity towards catechol. Moreover, the whole cells provided a better environment for C23O to maintain its catalytic activity and stability compared with crude enzyme...
October 26, 2018: Biosensors & Bioelectronics
K Kamil Reza, Abu Ali Ibn Sina, Alain Wuethrich, Yadveer S Grewal, Christopher B Howard, Darren Korbie, Matt Trau
Immune checkpoint blockade therapies are promising next generation immunotherapeutic treatments for cancer. Whilst sequential solid biopsies are an invaluable source of prognostic information, they are not feasible for monitoring therapeutic outcomes over time. Monitoring soluble immune checkpoint markers expression in body fluids could potentially be a better alternative. Current methods (e.g. ELISA) for detecting immune-checkpoint proteins mostly rely on the use of monoclonal antibodies which are expensive and time-consuming to manufacture and isolate...
October 25, 2018: Biosensors & Bioelectronics
Haiyang Li, Cheng Wang, Xiaodong Wang, Peichen Hou, Bin Luo, Peng Song, Dayu Pan, Aixue Li, Liping Chen
In vivo detecting of plants signal molecules is of great importance for the precision farming, crop management and plant phenotyping. In this work, for in vivo detecting indole-3-acetic acid (IAA), one of phytohormones, fine stainless steel (SS) wire was used as electrode material. Highly ordered nanopores, popcorn-like Au nanostructures, Pt nanoparticles and reduced graphene oxide (ERGO) nanocomposite films, and polymerized ST film (PST) were fabricated on the SS microelectrode in turn for improving the detection effect...
October 24, 2018: Biosensors & Bioelectronics
Yijun Li, Yibo Zhu, Cheng Wang, Miao He, Qiao Lin
Graphene field-effect transistor (GFET) sensors are an attractive analytical tool for the detection of water pollutants. Unfortunately, this application has been hindered by the sensitivity of such sensors to nonspecific disturbances caused by variations of environmental conditions. Incorporation of differential designs is a logical choice to address this issue, but this has been difficult for GFET sensors due to the impact of fabrication processes and material properties. This paper presents a differential GFET affinity sensor for the selective detection of water pollutants in the presence of nonspecific disturbances...
October 24, 2018: Biosensors & Bioelectronics
Srivithya Vellampatti, Maddaka Reddeppa, Sreekantha Reddy Dugasani, Sekhar Babu Mitta, Bramaramba Gnapareddy, Moon-Deock Kim, Sung Ha Park
Even though lanthanide ion (Ln3+ )-doped DNA nanostructures have been utilized in various applications, they are rarely employed for photovoltage generating devices because of difficulties in designing DNA-based devices that generate voltages under light illumination. Here, we constructed DNA lattices made of synthetic strands and DNA thin films extracted from salmon (SDNA) with single-doping of Nd3+ or Er3+ and co-doping of Nd3+ /Er3+ for high performance UV detection. The topological change of the DNA double-crossover (DX) lattices during the course of annealing was estimated from atomic force microscope (AFM) images to find the optimum concentration of Ln3+ ([Ln3+ ]O )...
October 24, 2018: Biosensors & Bioelectronics
Yifeng Zhang, Mengdi Wang, Yaoguang Wang, Jinhui Feng, Yong Zhang, Xu Sun, Bin Du, Qin Wei
In this study, we propose a SnO2 /CdCO3 /CdS-based label-free photoelectrochemical (PEC) immunosensor for the sensitive quantification of amyloid β-protein (Aβ) which plays a critical role in the early diagnosis of Alzheimer's disease. Under specific pH conditions, SnO2 /CdCO3 /CdS was first designed and successfully synthesized with simple one-pot method. The synthesis of SnO2 /CdCO3 /CdS circumvents the trouble of traditional composite materials to be gradually compounded, saving a lot of time and energy...
October 24, 2018: Biosensors & Bioelectronics
Roshan Khadka, Nihan Aydemir, Colm Carraher, Cyril Hamiaux, Damon Colbert, Jamal Cheema, Jenny Malmström, Andrew Kralicek, Jadranka Travas-Sejdic
Herein, we present that insect odorant receptors reconstituted into the lipid bilayers of liposomes can be successfully immobilized onto a gold surface and selectively and sensitively detect odorant molecules. The odorant receptors (OrXs) Or10a, Or22a, and Or71a from the common fruit fly, Drosophila melanogaster, were recombinantly expressed, purified and integrated into nano-liposomes (100-200 nm). These liposomes were covalently attached to the self-assembled monolayers (SAMs) of a 6-mercaptohexanoic acid (MHA)-modified gold surface...
October 23, 2018: Biosensors & Bioelectronics
Bang Hyun Lee, Sang Hoon Kim, Youngkyung Ko, Joo Cheol Park, Suk Ji, Man Bock Gu
This research aims to develop biosensors which could diagnose periodontal diseases in early phases and predict the illness stage of patients, in order to give them adequate treatment timely. Human odontogenic ameloblast-associated protein (ODAM) is considered to be a potential biomarker for periodontal diseases, based on high correlation between the level of ODAM in gingival crevicular fluid (GCF) and the degree of periodontitis. Many aptamers, including a cognate pair of aptamers which can bind to the different sites of ODAM, were successfully screened in a very stringent condition employing saliva as a counter target through the graphene oxide-based systemic evolution of ligands by exponential enrichment (GO-SELEX)...
October 23, 2018: Biosensors & Bioelectronics
Saif Mohammad Ishraq Bari, Louis G Reis, Gergana G Nestorova
We report a lab-on-a-chip immunosesnor for quantification of the inflammatory cytokine TNF-α with picomolar sensitivity. The feasibility of the technology was demonstrated via accurate measurement of the concentration of TNF-α in astrocytes cell culture media. The immunoassay was performed in a microfluidic device with an integrated antimony/bismuth thermopile sensor and had a limit of detection of 14 pg mL-1 . The device was fabricated using rapid prototyping xurography technique and consisted of two inlets and single outlet...
October 22, 2018: Biosensors & Bioelectronics
Somasekhar R Chinnadayyala, Jinsoo Park, Hien Thi Ngoc Le, Mallesh Santhosh, Abhijit N Kadam, Sungbo Cho
Electrogenerated chemiluminescence (ECL) is an effective method for detecting a wide range of analytes including metal ions, virulent DNA, pathogenic bacteria, tumor cells and glucose. The attractive features of paper including passive liquid transport and biocompatibility are the main two advantages of using paper as a biosensing platform. To achieve key factors in paper-based sensors, the fabrication procedures and the analysis methods are fine tuned to satisfy the requirements of the ultimate-users. Here, we review various ECL signal amplification labels, inexpensive and portable devices, such as rechargeable batteries, which have replaced traditional instrumentation and different light detection technologies used in paper ECL devices...
October 22, 2018: Biosensors & Bioelectronics
Shan Liang, Lidong Wu, Huan Liu, Jincheng Li, Mengze Chen, Min Zhang
Black phosphorus (BP), also known as phosphorene (PP), is a fascinating two-dimensional (2D) material with extraordinary anisotropic mechanical, electronic and optoelectronic properties. However, PP is sensitive to oxygen and moisture and is completely degenerated by oxygen and humid air within 12 h, which limits its applications. Here, we coat PP with hexamethylenediamine (HMA), which allows the coated PP to maintain its original form in aqueous solution for over one month. The stable PP is dotted with gold nanoparticles to facilitate binding to a 3,3'4,4'-polychlorinated biphenyl (PCB77) aptamer (ap) as a biosensor...
October 21, 2018: Biosensors & Bioelectronics
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