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Biosensors & Bioelectronics

Alejandro Zamora-Gálvez, Eden Morales-Narváez, Javier Romero, Arben Merkoçi
A new paper-based lateral flow immunoassay configuration was engineered and investigated. The assay is intended for the detection of a model protein in human serum, that is, human immunoglobulin G, with the aim to demonstrate a virtually universal protein detection platform. Once the sample is added in the strip, the analyte is selectively captured by antibody-decorated silica beads (Ab-SiO2) onto the conjugate pad and the sample flows by capillarity throughout the strip until reaching the test line, where a sandwich-like immunocomplex takes place due to the presence of antibody-functionalized QDs (Ab-QDs) onto the test line...
September 11, 2017: Biosensors & Bioelectronics
Wei Diao, Min Tang, Shijia Ding, Xinmin Li, Wenbin Cheng, Fei Mo, Xiaoyu Yan, Hongmin Ma, Yurong Yan
Early detection, diagnosis and treatment of human immune deficiency virus (HIV) infection is the key to reduce acquired immunodeficiency syndrome (AIDS) mortality. In our research, an innovative surface plasmon resonance (SPR) biosensing strategy has been developed for highly sensitive detection of HIV-related DNA based on entropy-driven strand displacement reactions (ESDRs) and double-layer DNA tetrahedrons (DDTs). ESDRs as enzyme-free and label-free signal amplification circuit can be specifically triggered by target DNA, leading to the cyclic utilization of target DNA and the formation of plentiful double-stranded DNA (dsDNA) products...
September 9, 2017: Biosensors & Bioelectronics
C S Pundir, Vinay Narwal
Triglycerides (TGs) are the major transporters of dietary fats throughout the bloodstream. Besides transporting fat, TGs also act as stored fat in adipose tissue, which is utilized during insufficient carbohydrates supply. TG level is below 150mg/dL in healthy persons. Elevated TGs level in blood over 500mg/dL is a biomarker for cardiovascular diseases, Alzheimer disease, pancreatitis and diabetes. Numerous methods are accessible for recognition of TGs, among them, most are cumbersome, time-consuming, require sample pre-treatment, high cost instrumental set-up and experienced personnel to operate...
September 9, 2017: Biosensors & Bioelectronics
Uchangi Satyaprasad Akshath, Praveena Bhatt
Supramolecular nanoparticle hybrids for biosensing of analytes have been a major focus due to their tunable optical and surface properties. Quantum dots-Gold nanoparticle (QDs-GNP) based FRET probes involving turn on/off principles have gained immense interest due to their specificity and sensitivity. Recent focus is on applying these supramolecular hybrids for enzyme operated biosensors that can specifically turn-on fluorescence induced by co-factor or product formed from enzymatic reaction. The present study focuses on locking and unlocking the interaction between QD-GNP pair leading to differential fluorescent properties...
September 9, 2017: Biosensors & Bioelectronics
Yongliang Wang, Dana N LeVine, Margaret Gannon, Yuanchang Zhao, Anwesha Sarkar, Bailey Hoch, Xuefeng Wang
Integrin-transmitted cellular forces are critical for platelet adhesion, activation, aggregation and contraction during hemostasis and thrombosis. Measuring and mapping single platelet forces are desired in both research and clinical applications. Conventional force-to-strain based cell traction force microscopies have low resolution which is not ideal for cellular force mapping in small platelets. To enable platelet force mapping with submicron resolution, we developed a force-activatable biosensor named integrative tension sensor (ITS) which directly converts molecular tensions to fluorescent signals, therefore enabling cellular force mapping directly by fluorescence imaging...
September 8, 2017: Biosensors & Bioelectronics
Li Zhang, Xiaofen Xiao, Yuzhi Xu, Danping Chen, Jun Chen, Yingjun Ma, Zong Dai, Xiaoyong Zou
A simple electrochemical strategy is reported for continuous monitoring of dynamic DNA methylation process over time. An electrochemical sensor was prepared by co-assembling of DNA probe and 6-mercapto-1-hexanol onto a gold electrode. The top of the DNA probe was labeled with 6-ferrocenylhexanethiol modified gold nanoparticle. The charge density between the C•G base pair was verified to be slightly reduced by DNA methylation, and could be further decelerated by ~ 25% upon co-locating a Br group onto methylated cytosine (mC)...
September 4, 2017: Biosensors & Bioelectronics
Giovanni Palomino-Vizcaino, Diana Gabriela Valencia Reséndiz, María Luisa Benítez-Hess, Natalia Martínez-Acuña, Juana Virginia Tapia-Vieyra, Daniel Bahena, Mauricio Díaz-Sánchez, Octavio Patricio García-González, Brenda Arizaí Alvarez-Sandoval, Luis Marat Alvarez-Salas
Colorimetric assays based on gold nanoparticles (GNPs) are of considerable interest for diagnostics because of their simplicity and low-cost. Nevertheless, a deep understanding of the interaction between the GNPs and the intended molecular target is critical for the development of reliable detection technologies. The present report describes the spontaneous interaction between HPV16 L1 virus-like particles (VLPs) and non-functionalized GNPs (nfGNPs) resulting in the inhibition of nfGNPs salt-induced aggregation and the stabilization of purified VLPs...
September 4, 2017: Biosensors & Bioelectronics
Tong Liu, Li-Li Liang, Peng Xiao, Li-Peng Sun, Yun-Yun Huang, Yang Ran, Long Jin, Bai-Ou Guan
Fiber optics evanescent field based biosensor is an excellent candidate for label-free detection of cardiac biomarkers which is of great importance in rapid, early, and accurate diagnosis of acute myocardial infarction (AMI). In this paper, we report a compact and sensitive cardiac troponin I (cTn-I) immunosensor based on the phase-shifted microfiber Bragg grating probe which is functionalized. The fine reflective signal induced by the phase shift in modulation significantly improves the spectral resolution, enabling the ability of the sensor in perceiving an ultra-small refractive index change due to the specific capture of the cTn-I antigens...
September 4, 2017: Biosensors & Bioelectronics
Binbin Luo, Yangfei Xu, Shengxi Wu, Mingfu Zhao, Pengjun Jiang, Shenghui Shi, Zhonghao Zhang, Yin Wang, Linlin Wang, Yong Liu
A novel immunosensor for detecting Newcastle disease virus (NDV) was developed using excessively tilted fiber grating (Ex-TFG) coated with gold nanospheres (AuNs). AuNs were coated on the Ex-TFG surface via Au-S bonds using 3-mercaptopropyltrimethoxysilane (MPTMS), and the activated staphylococcal protein A (SPA) was linked to AuNs by covalent bonds via cysteamine. AuNs greatly enhanced the impact of the analyte on the fiber cladding mode through the local surface Plasmon resonance (LSPR) effect, thus improving the detection limit and sensitivity of the immunosensor...
September 1, 2017: Biosensors & Bioelectronics
Hongmei Wang, Qin Xu, Juan Wang, Wei Du, Fengping Liu, Xiaoya Hu
In this work, a novel molecularly imprinted electrochemical sensor based on the amino-functionalized silica nanoparticles was built for the sensitive and selective detection of 2,4-dichlorophenoxyacetic acid (2,4-D). The hierarchical porous dendrimer-like silica nanoparticles (HPSNs-NH2) were synthesized by an ethyl ether emulsion method. The selective molecularly imprinted polymers (MIP) was prepared on the HPSNs-NH2 modified electrode via electropolymerization by using 2,4-D as the template and o-phenylenediamine (OPD) as the monomer...
September 1, 2017: Biosensors & Bioelectronics
Mohammed Y Emran, Moataz Mekawy, Naeem Akhtar, Mohamed A Shenashen, Islam M El-Sewify, Ahmed Faheem, Sherif A El-Safty
Monitoring and determination of ultra-trace concentrations of monoamine neurotransmitter such as noradrenaline (NA) in living cells with simple, sensitive and selective assays are significantly interesting. We design NA-electrode sensing system based on C-, N-doped NiO broccoli-like hierarchy (CNNB). The spherical broccoli-head umbrella architectures associated with nano-tubular arrangements enabled to tailor NA biosensor design. The homogenous doping and anisotropic dispersion of CN nanospheres along the entire NB head nanotubes lead to creating of abundant electroactive sites in the interior tubular vessels and outer surfaces for ultrasensitive detection of NA in living cells such as PC12...
August 31, 2017: Biosensors & Bioelectronics
Abkar Sayad, Fatimah Ibrahim, Shah Mukim Uddin, Jongman Cho, Marc Madou, Kwai Lin Thong
Outbreaks of foodborne diseases have become a global health concern; hence, many improvements and developments have been made to reduce the risk of food contamination. We developed a centrifugal microfluidic automatic wireless endpoint detection system integrated with loop mediated isothermal amplification (LAMP) for monoplex pathogen detection. Six identical sets were designed on the microfluidic compact disc (CD) to perform 30 genetic analyses of three different species of foodborne pathogens. The consecutive loading, mixing, and aliquoting of the LAMP primers/reagents and DNA sample solutions were accomplished using an optimized square-wave microchannel, metering chambers and revulsion per minute (RPM) control...
August 31, 2017: Biosensors & Bioelectronics
Anil H Valekar, Bhagwan S Batule, Moon Il Kim, Kyung-Ho Cho, Do-Young Hong, U-Hwang Lee, Jong-San Chang, Hyun Gyu Park, Young Kyu Hwang
We herein describe novel amine-grafted metal-organic frameworks (MOFs) as a promising alternative to natural peroxidase enzyme and their applications for a fluorescent assay of choline (Cho) and acetylcholine (ACh). Among diverse amine-functionalized MOFs, N,N,N',N'-tetramethyl-1,4-butanediamine (TMBDA)-functionalized MIL-100(Fe) (TMBDA-MIL-100(Fe)) exhibited the highest peroxidase activity by developing intense fluorescence from Amplex UltraRed (AUR) in the presence of H2O2, which was presumably due to the synergetic effect of the enhanced negative potential and precisely controlled molecular size of the grafted diamine...
August 30, 2017: Biosensors & Bioelectronics
Tugba Kilic, Valerie Brunner, Laurent Audoly, Sandro Carrara
Schizophrenia treatment may see a paradigm shift due to development of new atypical antipsychotic drugs (APDs), with better tolerability due to more selective dopamine (DA) receptor blockade. Monitoring of these APD candidates in biological fluids is of great importance to reduce the development cost, to clarify the mechanism of action and ultimately to support the demonstration of efficacy of these molecules. Electrochemical approaches have attracted great attention for monitoring DA and APD levels but none of the methods developed so far aimed to screen APD candidates...
August 30, 2017: Biosensors & Bioelectronics
Tingting Gu, Wu Zou, Fuchun Gong, Jiaoyun Xia, Can Chen, Xuejiao Chen
As a new member of the carbon quantum-dot family, fluorescent nitrogen-rich quantum dots (NRQDs) were prepared by a mixed solvothermal method using 2-azidoimidazole and aqueous ammonia as reactants. These NRQDs are rich in nitrogen up to 40.2%, which are endowed with high fluorescence quantum yield, good photostability, water-solubility and favourable biocompatibility. We further explored the use of NRQDs combined with Cu(2+) as a nanoprobe for sensing fluorescently of cysteine (Cys) in complex biological samples...
August 29, 2017: Biosensors & Bioelectronics
Rijun Gui, Hui Jin, Huijun Guo, Zonghua Wang
Molecularly imprinted polymers (MIPs)-based electrochemical biosensors (ECBSs) have many advantages from MIPs and ECBSs, such as high selectivity and sensitivity, chemical/mechanical stability, reusability, low limit of detection, facile preparation and low cost. MIPs-based ECBSs attract much attention in medical diagnose, biological analysis, environmental monitoring, food safety evaluation, etc. Due to the capacity of highly specific recognition for target biomolecules, MIPs-based ECBSs have been smartly designed and extensively used for electrochemical sensing applications in recent years, exhibiting obvious superiority over other analytical techniques...
August 29, 2017: Biosensors & Bioelectronics
Bi-Yun Fang, Cheng Li, Yuan-Yang Song, Fang Tan, Yuan-Cheng Cao, Yuan-Di Zhao
Graphene quantum dot (GQD) has been attractive in analytical science field due to its low toxicity, stable photoluminescence. Herein, nitrogen-doped GQD (N-GQD) was prepared by a facile solvothermal treatment of GO using dimethylformamide, and exhibited a green emission with 23.1% quantum yield. The N-GQD probe showed a selective and sensitive fluorescence enhancement response to Al(3+), the mechanism might be the formation of a complex between Al(3+) and N-GQD constrained the photo-induced electron transfer (PET) process of N-GQD itself...
August 26, 2017: Biosensors & Bioelectronics
Haijun Wang, Yaqin Chai, Hang Li, Ruo Yuan
Electrochemiluminescence (ECL) co-reaction and ECL resonance energy transfer (ECL-RET) are two main methods for enhancing the ECL efficiency. In this work, a novel tris(bipyridine) ruthenium(II) derivative (Ru(bpy)2(mcbpy)(2+)-PEI-ABEI) with high ECL efficiency due to the binary intramolecular ECL self-catalyzed property including intramolecular co-reaction and intramolecular ECL-RET, was prepared for the construction of immunosensor. Firstly, through intramolecular co-reaction, polyethyleneimine (PEI) with large amount of amine co-reactive groups (primary, secondary and tertiary amine) not only increased the ECL intensity of Ru(bpy)2(mcbpy)(2+), but also improved the ECL signal of N-(aminobutyl)-N-(ethylisoluminol) (ABEI)...
August 26, 2017: Biosensors & Bioelectronics
Danni Hao, Chunxiao Hu, James Grant, Andrew Glidle, David R S Cumming
We report on the design and fabrication of a hybrid sensor that integrates transmission-mode localized surface plasmonic resonance (LSPR) into a quartz crystal microbalance (QCM) for studying biochemical surface reactions. The coupling of LSPR nanostructures and a QCM allows optical spectra and QCM resonant frequency shifts to be recorded simultaneously and analyzed in real time for a given surface adsorption process. This integration simplifies the conventional combination of SPR and QCM and has the potential to be miniaturized for application in point-of-care (POC) diagnostics...
August 26, 2017: Biosensors & Bioelectronics
Qian Qian Zhang, Bin Bin Chen, Hong Yan Zou, Yuan Fang Li, Cheng Zhi Huang
Hematin plays a crucial role in various physiological functions, and the determination of hematin in complex biological matrixes is a significant but difficult issue. Considering the unique photophysical/photochemical properties of carbon quantum dots (CQDs) prepared with p-aminobenzoic acid (PABA) and ethanol, a new strategy for the design of fluorescent probes for hematin has been achieved. The proposed sensor array is fabricated based on the inner filter effect (IFE) between hematin and CQDs with phenomenon of selective fluorescence quenching of CQDs which results from the strong absorption of the excitation and emission spectrum of CQDs by hematin...
August 24, 2017: Biosensors & Bioelectronics
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