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Biosensors & Bioelectronics

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https://www.readbyqxmd.com/read/28525853/photoelectrochemical-sensitive-detection-of-insulin-based-on-cds-polydopamine-co-sensitized-wo3-nanorod-and-signal-amplification-of-carbon-nanotubes-polydopamine
#1
Rongyu Wang, Hongmin Ma, Yong Zhang, Qi Wang, Zhongping Yang, Bin Du, Dan Wu, Qin Wei
An ultrasensitive photoelectrochemical sandwich immunosensor was designed for detection of insulin based on WO3/CdS/polydopamine (WO3/CdS/PDA) co-sensitized and PDA@carbon nanotubes (PDA@CNT) conjugates for signal amplification. The CdS nanoparticles were first deposited on the WO3 nanorods via sequential chemical bath deposition to form the WO3/CdS structure to enhance photocurrent. Then equipped with PDA to form the WO3/CdS/PDA photosensitive structure. The PDA was used not only to reduce the toxicity of CdS but also adsorb insulin primary antibodies (Ab1)...
May 13, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28525849/novel-photoelectrochemical-immunosensor-for-disease-related-protein-assisted-by-hemin-g-quadruplex-based-dnazyme-on-gold-nanoparticles-to-enhance-cathodic-photocurrent-on-p-cubi2o4-semiconductor
#2
Shuzhen Lv, Kangyao Zhang, Zhenzhen Lin, Dianping Tang
A novel p-type semiconductor material (p-CuBi2O4) is designed for the construction of split-type photoelectrochemical (PEC) immunosensor for alpha-fetoprotein (AFP) with the hemin assistant to enhance the cathodic photocurrent. Initially, the photocathode of PEC immunosensor is fabricated by p-CuBi2O4 on a layer of gold nanoparticles (AuNPs, as a front contact of p-CuBi2O4) to enhance the efficiency of charge separation. In the presence of target AFP, a sandwich-type immunoreaction was carried out in capture antibody-coated microplate by using detection antibody and hemin-based G-quadruplex (labeled on the AuNP) as the signal probe...
May 13, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28525854/sensitive-sulfide-ion-detection-by-optofluidic-catalytic-laser-using-horseradish-peroxidase-hrp-enzyme
#3
Chaoyang Gong, Yuan Gong, Maung Kyaw Khaing Oo, Yu Wu, Yunjiang Rao, Xiaotian Tan, Xudong Fan
We report an optofluidic catalytic laser for sensitive sulfide ion detection. In the catalytic reaction, horseradish peroxidase (HRP) enzyme is used for catalyzing the non-fluorescent substrate, 10-Acetyl-3,7-dihydroxyphenox-azine (ADHP), to produce highly fluorescent resorufin, which was used as gain medium for lasing. Using sulfide ions as inhibitors, the catalytic reaction slows down, resulting in a delay in the lasing onset time, which is used as the sensing signal. The sensing mechanism of the catalytic laser is theoretically analyzed and the performance is experimentally characterized...
May 12, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28525852/absolute-quantification-of-dna-methylation-using-microfluidic-chip-based-digital-pcr
#4
Zhenhua Wu, Yanan Bai, Zule Cheng, Fangming Liu, Ping Wang, Dawei Yang, Gang Li, Qinghui Jin, Hongju Mao, Jianlong Zhao
Hypermethylation of CpG islands in the promoter region of many tumor suppressor genes downregulates their expression and in a result promotes tumorigenesis. Therefore, detection of DNA methylation status is a convenient diagnostic tool for cancer detection. Here, we reported a novel method for the integrative detection of methylation by the microfluidic chip-based digital PCR. This method relies on methylation-sensitive restriction enzyme HpaII, which cleaves the unmethylated DNA strands while keeping the methylated ones intact...
May 12, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28525850/highly-sensitive-aptasensor-based-on-fluorescence-resonance-energy-transfer-between-l-cysteine-capped-zns-quantum-dots-and-graphene-oxide-sheets-for-the-determination-of-edifenphos-fungicide
#5
Majid Arvand, Aazam A Mirroshandel
With the advantages of excellent optical properties and biocompatibility, single-strand DNA-functionalized quantum dots have been widely applied in biosensing and bioimaging. A new aptasensor with easy operation, high sensitivity, and high selectivity was developed by immobilizing the aptamer on water soluble l-cysteine capped ZnS quantum dots (QDs). Graphene oxide (GO) sheets are mixed with the aptamer-QDs. Consequently, the aptamer-conjugated QDs bind to the GO sheets to form a GO/aptamer-QDs ensemble. This aptasensor enables the energy transfer based on a fluorescence resonance energy transfer (FRET) from the QDs to the GO sheets, quenching the fluorescence of QDs...
May 12, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28511112/real-time-fluorescence-assay-of-alkaline-phosphatase-in-living-cells-using-boron-doped-graphene-quantum-dots-as-fluorophores
#6
Li Chen, Guancao Yang, Ping Wu, Chenxin Cai
This work reports a convenient and real-time assay of alkaline phosphatase (ALP) in living cells based on a fluorescence quench-recovery process at a physiological pH using the boron-doped graphene quantum dots (BGQDs) as fluorophore. The fluorescence of BGQDs is found to be effectively quenched by Ce(3+) ions because of the coordination of Ce(3+) ions with the carboxyl group of BGQDs. Upon addition of adenosine triphosphate (ATP) into the system, the quenched fluorescence can be recovered by the ALP-positive expressed cells (such as MCF-7 cells) due to the removal of Ce(3+) ions from BGQDs surface by phosphate ions, which are generated from ATP under catalytic hydrolysis of ALP that expressed in cells...
May 12, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28505563/ultrasensitive-magnetic-field-assisted-surface-plasmon-resonance-immunoassay-for-human-cardiac-troponin-i
#7
Qiong Wu, Ying Sun, Di Zhang, Shuo Li, Yue Zhang, Pinyi Ma, Yang Yu, Xinghua Wang, Daqian Song
An ultrasensitive surface plasmon resonance (SPR) immunoassay was developed for the specific detection of human cardiac troponin I (cTnI), a principle diagnostic marker for myocardial damage. The thin gold film evaporated on a glass slate, which was employed as the SPR sensing film, was modified by hollow gold nanoparticles (HGNPs) and polydopamine (PDA) sequentially, and then was immobilized with antibodies for specific recognition of target analyte. Electronic coupling of the surface plasmon waves originating from the HGNPs and the gold film leads to the remarkable amplification of SPR response...
May 12, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28527412/early-stage-detection-of-ve-cadherin-during-endothelial-differentiation-of-human-mesenchymal-stem-cells-using-spr-biosensor
#8
Farzaneh Fathi, Aysa Rezabakhsh, Reza Rahbarghazi, Mohammad-Reza Rashidi
Surface plasmon resonance (SPR) biosensors are most commonly applied for real-time dynamic analysis and measurement of interactions in bio-molecular studies and cell-surface analysis without the need for labeling processes. Up to present, SPR application in stem cell biology and biomedical sciences was underused. Herein, a very simple and sensitive method was developed to evaluate human mesenchymal stem cells trans-differentiation to endothelial lineage of over a period of 14 days based on VE-cadherin biomarker...
May 10, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28511113/turn-on-theranostic-fluorescent-nanoprobe-by-electrostatic-self-assembly-of-carbon-dots-with-doxorubicin-for-targeted-cancer-cell-imaging-in-vivo-hyaluronidase-analysis-and-targeted-drug-delivery
#9
Na Gao, Wen Yang, Hailiang Nie, Yunqian Gong, Jing Jing, Loujun Gao, Xiaoling Zhang
This paper reports a turn-on theranostic fluorescent nanoprobe P-CDs/HA-Dox obtained by electrostatic assembly of polyethylenimine (PEI)-modified carbon dots (P-CDs) and Hyaluronic acid (HA)-conjugated doxorubicin (Dox) for hyaluronidase (HAase) detection, self-targeted imaging and drug delivery. P-CDs/HA-Dox show weak emission in a physiological environment. By utilizing the high affinity of HA to CD44 receptors overexpressed on many cancer cells, P-CDs/HA-Dox are capable of targeting and penetrating into cancer cells, where they are activated by HAase...
May 10, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28501746/a-label-free-nanostructured-plasmonic-biosensor-based-on-blu-ray-discs-with-integrated-microfluidics-for-sensitive-biodetection
#10
Gerardo A López-Muñoz, M-Carmen Estevez, E Cristina Peláez-Gutierrez, Antoni Homs-Corbera, M Carmen García-Hernandez, J Ignacio Imbaud, Laura M Lechuga
Nanostructure-based plasmonic biosensors have quickly positioned themselves as interesting candidates for the design of portable optical biosensor platforms considering the potential benefits they can offer in integration, miniaturization, multiplexing, and real-time label-free detection. We have developed a simple integrated nanoplasmonic sensor taking advantage of the periodic nanostructured array of commercial Blu-ray discs. Sensors with two gold film thicknesses (50 and 100nm) were fabricated and optically characterized by varying the oblique-angle of the incident light in optical reflectance measurements...
May 10, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28500948/using-reduced-graphene-oxide-ca-cdse-nanocomposite-to-enhance-photoelectrochemical-activity-of-gold-nanoparticles-functionalized-tungsten-oxide-for-highly-sensitive-prostate-specific-antigen-detection
#11
Xueping Wang, Rui Xu, Xu Sun, Yaoguang Wang, Xiang Ren, Bin Du, Dan Wu, Qin Wei
An ultrasensitive sandwich-type photoelectrochemical (PEC) immunosensor was constructed for the detection of prostate specific antigen (PSA). In this work, Au-nanoparticle-loaded tungsten oxide (WO3-Au) hybrid composites was applied as PEC sensing platform, while Ca ions doped CdSe equipped on the conducting framework of reduced graphene oxide (rGO-Ca:CdSe) nanocomposites were employed as the signal amplification probe. As for WO3-Au, massive Au nanoparticles were formed on the surface of WO3 without any additional reducing agent, providing a novel nanocarriers for anchoring plenty of the primary antibodies due to the large specific surface area and good biocompatibility by chemical bonding between Au nanoparticles and -NH2 of antibodies...
May 10, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28499468/corrigendum-to-ultrasensitive-and-selective-electrochemical-biosensor-for-detection-of-mercury-ii-ions-by-nicking-endonuclease-assisted-target-recycling-and-hybridization-chain-reaction-signal-amplification-biosens-bioelectron-94-2017-19-23
#12
https://www.readbyqxmd.com/read/28505562/ultra-fast-electronic-detection-of-antimicrobial-resistance-genes-using-isothermal-amplification-and-thin-film-transistor-sensors
#13
Chunxiao Hu, Sumit Kalsi, Ioannis Zeimpekis, Kai Sun, Peter Ashburn, Carrie Turner, J Mark Sutton, Hywel Morgan
A low cost thin-film transistor (TFT) nanoribbon (NR) sensor has been developed for rapid real-time detection of DNA amplification using an isothermal Recombinase Polymerase Amplification (RPA) method. The semiconductor chip measures DNA amplification through a pH change, rather than via fluorescence. The utility of the method was demonstrated by amplifying CTX-M and NDM, two genes that confer bacterial resistance to cephalosporins and carbapenems, respectively. It is shown that this approach provides extremely fast and sensitive detection...
May 9, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28505561/class-enzyme-based-motors-for-on-the-fly-enantiomer-analysis-of-amino-acids
#14
Laura García-Carmona, María Moreno-Guzmán, María Cristina González, Alberto Escarpa
Here, two class-enzyme motors are properly designed allowing the rapid dispersion of the class-enzyme D-amino acid oxidase (DAO) and L-amino acid oxidase (LAO) for selective "on the fly" biodetection of D and L-amino acids (AAs), respectively. The efficient movement together with the continuous release of fresh class-enzyme leads to a greatly accelerated enzymatic reaction processes without the need of external stirring or chemical and physical attachment of the enzyme. Ultra-fast detection (<2min) and accurate quantifications of L-phenylalanine (L-Phe) in plasma and whole-blood newborns samples diagnosed with Phenylketonuria and total D-AAs in Vibrio cholera cultures are pioneer illustrated as relevant examples of each enantiomer determination...
May 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28500946/pa-tb-cu-mof-as-luminescent-nanoenzyme-for-catalytic-assay-of-hydrogen-peroxide
#15
Zewan Qi, Li Wang, Qi You, Yang Chen
Metal organic frameworks (MOFs) with flexible structures and components have aroused great interest in designing functional materials. In this work, we designed and made a kind of PA-Tb-Cu MOF nanoenzyme capable of emitting fluorescence for the catalytic reaction of hydrogen peroxide (H2O2). Luminescent Tb(3+), catalytic Cu(2+) and bridging ligand were assembled and integrated into a single material nanoenzyme. This PA-Tb-Cu MOF nanoenzyme not only possessed excellent catalytic activity comparable to horseradish peroxidase but also can real-time fluorescently indicate the concentration of H2O2 as low as 0...
May 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28499195/using-the-rubik-s-cube-to-directly-produce-paper-analytical-devices-for-quantitative-point-of-care-aptamer-based-assays
#16
Haiyan Fu, Juanhua Yang, Lin Guo, Jinfang Nie, Qiaobo Yin, Lang Zhang, Yun Zhang
In this article, we describe a facile method named as Rubik's Cube stamping (RCS) for equipment-free fabrication of microfluidic paper-based analytical devices (μPADs). RCS is inspired by the worldwide ubiquitous RC toy and requires no specialized electric equipment other than a classical six-faced RC that is assembled with home-made small iron components. It can pattern various rosin microstructures in paper simply by either using different functional faces of the modified RC or applying its internal pivot mechanism to adjust the components' patterning forms on one functional face...
May 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28494370/a-novel-sensitive-sensor-for-serotonin-based-on-high-quality-of-auag-nanoalloy-encapsulated-graphene-electrocatalyst
#17
Tran Duy Thanh, Jayaraman Balamurugan, Hoa Van Hien, Nam Hoon Kim, Joong Hee Lee
A high quality graphene-encapsulated AuAg alloy (AuAg-GR) nanohybrid with homogeneous structure and good reproducibility over a desired area was successfully fabricated. Taking benefits of the unique architecture, such nanohybrid was employed as an efficient electrocatalyst for sensing application. The AuAg-GR based sensor could sensitively detected neurotransmitter serotonin (5-HT) with wide linear detection range (2.7nM to 4.82μM), very low detection limit (1.6nM), negligible interference, and excellent reproducibility...
May 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28525851/a-fully-integrated-distance-readout-elisa-chip-for-point-of-care-testing-with-sample-in-answer-out-capability
#18
Dan Liu, Xingrui Li, Junkai Zhou, Shibo Liu, Tian Tian, Yanling Song, Zhi Zhu, Leiji Zhou, Tianhai Ji, Chaoyong Yang
Enzyme-linked immunosorbent assay (ELISA) is a popular laboratory technique for detection of disease-specific protein biomarkers with high specificity and sensitivity. However, ELISA requires labor-intensive and time-consuming procedures with skilled operators and spectroscopic instrumentation. Simplification of the procedures and miniaturization of the devices are crucial for ELISA-based point-of-care (POC) testing in resource-limited settings. Here, we present a fully integrated, instrument-free, low-cost and portable POC platform which integrates the process of ELISA and the distance readout into a single microfluidic chip...
May 4, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28525848/an-amplified-comparative-fluorescence-resonance-energy-transfer-immunosensing-of-ca125-tumor-marker-and-ovarian-cancer-cells-using-green-and-economic-carbon-dots-for-bio-applications-in-labeling-imaging-and-sensing
#19
Somayeh Hamd-Ghadareh, Abdollah Salimi, Fardin Fathi, Saman Bahrami
CA125, is a marker in the clinical diagnosis of several cancers and currently is the best serum-based tumor marker for ovarian cancer. Here, we developed an ultrasensitive antibody-ssDNA aptamer sandwich-type fluorescence immunosensor for CA125 detection. Based on a novel signal amplification strategy the carbon dots (CDs) functionalized with aptamer (CD-aptamer) used as detection probe and PAMAM-Dendrimers/AuNPs was used for covalent attachment of CA125-antibody and completing the sandwich assay method. By measuring of fluorescence resonance energy transfer (FRET) signals between CDs and AuNPs as nanoquenchers, the fluorescence signal quenched during sandwich complex formed between anti-CA125, CA125 and CDs-Aptamer and decreasing of fluorescence response signal is related to CA125 concentrations...
May 4, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28500947/electrochemical-latent-redox-ratiometric-probes-for-real-time-tracking-and-quantification-of-endogenous-hydrogen-sulfide-production-in-living-cells
#20
Kesavan Manibalan, Veerappan Mani, Pu-Chieh Chang, Chih-Hung Huang, Sheng-Tung Huang, Kasper Marchlewicz, Suresh Neethirajan
Hydrogen sulfide (H2S) was discovered as a third gasotransmitter in biological systems and recent years have seen a growing interest to understand its physiological and pathological functions. However, one major limiting factor is the lack of robust sensors to quantitatively track its production in real-time. We described a facile electrochemical assay based on latent redox probe approach for highly specific and sensitive quantification in living cells. Two chemical probes, Azido Benzyl ferrocene carbamate (ABFC) and N-alkyl Azido Benzyl ferrocene carbamate (NABFC) composed of azide trigger group were designed...
May 4, 2017: Biosensors & Bioelectronics
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