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Biosensors & Bioelectronics

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https://www.readbyqxmd.com/read/28715793/monitoring-in-vivo-metabolic-flux-with-a-designed-whole-cell-metabolite-biosensor-of-shikimic-acid
#1
Heng Li, Chaoning Liang, Wei Chen, Jian-Ming Jin, Shuang-Yan Tang, Yong Tao
Knowledge of intracellular metabolite levels is important for the understanding of metabolic flux distributions. Whole-cell biosensors of key metabolites are ideal for the monitoring of carbon flow in important metabolic pathways, thus guiding metabolic engineering for microbial improvement. However, lack of biosensors for metabolites of interests has limited their applications. In this study, a genetically encoded whole-cell biosensor specifically responding to shikimic acid has been developed by screening a site-saturation mutagenesis library of the binding pocket of a uric acid-responsive regulatory protein...
July 11, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28715792/smartphone-based-cyclic-voltammetry-system-with-graphene-modified-screen-printed-electrodes-for-glucose-detection
#2
Daizong Ji, Lei Liu, Shuang Li, Chen Chen, Yanli Lu, Jiajia Wu, Qingjun Liu
Smartphone-based electrochemical devices have such advantages as the low price, miniaturization, and obtaining the real-time data. As a popular electrochemical method, cyclic voltammetry (CV) has shown its great practicability for quantitative detection and electrodes modification. In this study, a smartphone-based CV system with a simple method of electrode modification was constructed to perform electrochemical detections. The system was composed of these main portions: modified electrodes, portable electrochemical detector and smartphone...
July 11, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28711825/autonomous-electrochemical-biosensors-a-new-vision-to-direct-methanol-fuel-cells
#3
M Goreti F Sales, Lúcia Brandão
A new approach to biosensing devices is demonstrated aiming an easier and simpler application in routine health care systems. Our methodology considered a new concept for the biosensor transducing event that allows to obtain, simultaneously, an equipment-free, user-friendly, cheap electrical biosensor. The use of the anode triple-phase boundary (TPB) layer of a passive direct methanol fuel cell (DMFC) as biosensor transducer is herein proposed. For that, the ionomer present in the anode catalytic layer of the DMFC is partially replaced by an ionomer with molecular recognition capability working as the biorecognition element of the biosensor...
July 11, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28711029/an-oxygen-independent-and-membrane-less-glucose-biobattery-supercapacitor-hybrid-device
#4
Xinxin Xiao, Peter Ó Conghaile, Dónal Leech, Roland Ludwig, Edmond Magner
Enzymatic biofuel cells can generate electricity directly from the chemical energy of biofuels in physiological fluids, but their power density is significantly limited by the performance of the cathode which is based on oxygen reduction for in vivo applications. An oxygen-independent and membrane-less glucose biobattery was prepared that consists of a dealloyed nanoporous gold (NPG) supported glucose dehydrogenase (GDH) bioanode, immobilised with the assistance of conductive polymer/Os redox polymer composites, and a solid-state NPG/MnO2 cathode...
July 11, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28711028/fluorescence-based-cdte-nanosensor-for-sensitive-detection-of-cytochrome-c
#5
Rehab M Amin, Souad A Elfeky, Thomas Verwanger, Barbara Krammer
Cytochrome c (Cyt c) is commonly used as intrinsic biomarker for several characteristics of the cell such as respiration, energy level and apoptosis. In the present study a simple colorimetric sensor should be developed and tested for the real-time detection of Cyt c in living cells. We synthesized cadmium telluride quantum dots (CdTe QDs) capped with thioglycolic acid (TGA) as a fluorometric Cyt c nanosensor. The synthesized TGA/CdTe QDs nanosensor was characterized by Fourier transform infrared spectroscopy, transmission electron microscopy, and absorption as well as fluorescence spectrophotometry...
July 8, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28711027/high-throughput-and-label-free-parasitemia-quantification-and-stage-differentiation-for-malaria-infected-red-blood-cells
#6
Xiaonan Yang, Zhuofa Chen, Jun Miao, Liwang Cui, Weihua Guan
This work reports a high throughput and label-free microfluidic cell deformability sensor for quantitative parasitemia measurement and stage determination for Plasmodium falciparum-infected red blood cells (Pf-iRBCs). The sensor relies on differentiating the RBC deformability (a mechanical biomarker) that is highly correlated with the infection status. The cell deformability is measured by evaluating the transit time when each individual RBC squeezes through a microscale constriction (cross-section ~5µm×5µm)...
July 8, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28711026/recent-advancement-in-biosensors-technology-for-animal-and-livestock-health-management
#7
REVIEW
Suresh Neethirajan, Satish K Tuteja, Sheng-Tung Huang, David Kelton
The term biosensors encompasses devices that have the potential to quantify physiological, immunological and behavioural responses of livestock and multiple animal species. Novel biosensing methodologies offer highly specialised monitoring devices for the specific measurement of individual and multiple parameters covering an animal's physiology as well as monitoring of an animal's environment. These devices are not only highly specific and sensitive for the parameters being analysed, but they are also reliable and easy to use, and can accelerate the monitoring process...
July 8, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28709089/an-electrochemical-sensor-for-indole-in-plasma-based-on-mwcnts-chitosan-modified-screen-printed-carbon-electrode
#8
Mingchao Jin, Xiaoqing Zhang, Qianna Zhen, Yifan He, Xiao Chen, Wenjing Lyu, Runchuan Han, Min Ding
Indole is an essential metabolite in intestinal tract. The dysregulation of plasma indole concentration occurred in various diseases. In this study, the indole in plasma was determined directly using electrochemical sensor with multiwall carbon nanotubes-chitosan (MWCNTs-CS) modified screen-printed carbon electrode (SPCE). The electrochemical behavior of indole was elucidated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) on the MWCNTs-CS composites modified SPCE (MWCNTs-CS/SPCE). The results showed that the current responses of indole improved greatly due to the high catalytic activity and electron transfer reaction of nano-composites...
July 8, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28697442/portable-amperometric-immunosensor-for-histamine-detection-using-prussian-blue-chitosan-gold-nanoparticle-nanocomposite-films
#9
Xiu-Xiu Dong, Jin-Yi Yang, Lin Luo, Yi-Feng Zhang, Chuanbin Mao, Yuan-Ming Sun, Hong-Tao Lei, Yu-Dong Shen, Ross C Beier, Zhen-Lin Xu
Histamine (HA) is a biogenic amine that can accumulate to high concentration levels in food as a result of microbial activity and can cause toxic effects in consumers. In this work, a portable electrochemical immunosensor capable of detecting HA with high sensitivity and selectivity was developed. Prussian blue-chitosan-gold nanoparticle (PB-CS-AuNP) nanocomposite films with excellent biocompatibility were synthesized and characterized by scanning electron microscopy and energy dispersive X-ray analysis. The PB-CS-AuNP were coated onto a screen-printed electrode by one-step electrodeposition and used to conjugate the HA ovalbumin conjugate (HA-Ag)...
July 8, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28709087/novel-electrochemiluminescence-of-silver-nanoclusters-fabricated-on-triplex-dna-scaffolds-for-label-free-detection-of-biothiols
#10
Lingyan Feng, Li Wu, Feifei Xing, Lianzhe Hu, Jinsong Ren, Xiaogang Qu
Electrochemiluminescence (ECL) of metal nanoclusters and their application have been widely reported due to the good biocompatibility, fascinating electrocatalytic activity and so on. Using DNA as synthesis template opens new opportunities to modulate the physical properties of AgNCs. Triplex DNA has been reported for the site-specific, homogeneous and highly stable silver nanoclusters (AgNCs) fabrication from our recent research. Here we further explore their extraordinary ECL properties and applications in biosensor utilization...
July 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28709086/sensitive-detection-of-brucine-an-anti-metastatic-drug-for-hepatocellular-carcinoma-at-carbon-nanotubes-nafion-composite-based-biosensor
#11
Rutesh Savalia, Sanghamitra Chatterjee
A neoteric approach for the electrochemical analysis of brucine in biological environment has been developed. The glassy carbon electrode modified with single walled carbon nanotubes and nafion composite film is delineated for the first time to determine brucine employing square wave voltammetry. The quantification of brucine at physiological pH 7.2 manifests remarkable performance at the developed biosensor. The effect of several operational parameters has been studied in the present investigation. Under optimized conditions, a dynamic linear range from 1nM to 8µM with a high sensitivity of 340...
July 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28704782/electrochemical-dna-biosensor-based-on-grafting-to-mode-of-terminal-deoxynucleoside-transferase-mediated-extension
#12
Jinyuan Chen, Zhoujie Liu, Huaping Peng, Yanjie Zheng, Zhen Lin, Ailin Liu, Wei Chen, Xinhua Lin
Previously reported electrochemical DNA biosensors based on in-situ polymerization approach reveal that terminal deoxynucleoside transferase (TdTase) has good amplifying performance and promising application in the design of electrochemical DNA biosensor. However, this method, in which the background is significantly affected by the amount of TdTase, suffers from being easy to produce false positive result and poor stability. Herein, we firstly present a novel electrochemical DNA biosensor based on grafting-to mode of TdTase-mediated extension, in which DNA targets are polymerized in homogeneous solution and then hybridized with DNA probes on BSA-based DNA carrier platform...
July 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28697446/electrochemical-peptide-sensor-for-diagnosing-adenoma-carcinoma-transition-in-colon-cancer
#13
Jong Min Lim, Myung Yi Ryu, Jong Won Yun, Tae Jung Park, Jong Pil Park
Colorectal cancer (CRC) is one of the leading causes of cancer-related deaths. Therefore, more sensitive and early diagnostic methods for CRC are urgently needed. In this study, an efficient electrochemical biosensor for early diagnosis of adenoma-to-carcinoma progression that employs a series of chemically modified affinity peptides was developed. A series of amino acid-substituted and cysteine-incorporated synthetic peptides with flexible linkers was chemically synthesized and immobilized to a gold sensor layer; performance of the sensor was monitored using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS)...
July 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28697443/selective-binding-of-pb-2-with-manganese-terephthalic-acid-mof-swcnts-theoretical-modeling-experimental-study-and-electroanalytical-application
#14
Fuxian Cai, Qinghua Wang, Xiaoqian Chen, Weiwei Qiu, Fengping Zhan, Fei Gao, Qingxiang Wang
A nanocomposite of flake-shaped manganese-terephthalic acid MOF/single-walled carbon nanotubes (Mn(TPA)-SWCNTs) was synthesized using manganese chloride, terephthalic acid and SWCNTs as raw materials. Theoretical modeling study shows that the Mn(TPA) component has stronger adsorption ability to lead ion (Pb(2+)) than the other common heavy metal ions, which is in good agreement with the result of electrochemical assay. Then, the Mn(TPA)-SWCNTs were used as a sensing matrix for the voltammetric determination of Pb(2+)...
July 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28711826/detection-principles-of-biological-and-chemical-fet-sensors
#15
REVIEW
Matti Kaisti
The seminal importance of detecting ions and molecules for point-of-care tests has driven the search for more sensitive, specific, and robust sensors. Electronic detection holds promise for future miniaturized in-situ applications and can be integrated into existing electronic manufacturing processes and technology. The resulting small devices will be inherently well suited for multiplexed and parallel detection. In this review, different field-effect transistor (FET) structures and detection principles are discussed, including label-free and indirect detection mechanisms...
July 5, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28709088/integrated-amplified-aptasensor-with-in-situ-precise-preparation-of-copper-nanoclusters-for-ultrasensitive-electrochemical-detection-of-microrna-21
#16
Yijia Wang, Xinying Zhang, Lang Zhao, Ting Bao, Wei Wen, Xiuhua Zhang, Shengfu Wang
MicroRNA 21 (MIR21) has garnered much attention in recent years as an important disease biomarker. The detection of it in human system shows great significance for the healthy evaluation and major diseases early detection. Herein, a novel approach tactfully manipulates the in-situ precise preparation of copper nanoclusters on overlapping Y-shaped ds-DNA for MIR21 analysis were developed in the proposed integrated aptasensor. In the presence of target MIR21, overlapping Y-shaped ds-DNA was constructed on electrode...
July 5, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28709085/amplification-of-electrochemical-signal-by-a-whole-cell-redox-reactivation-module-for-ultrasensitive-detection-of-pyocyanin
#17
Yuan Yang, Yang-Yang Yu, Yan-Zhai Wang, Chun-Lian Zhang, Jing-Xian Wang, Zhen Fang, Huoyang Lv, Jian-Jiang Zhong, Yang-Chun Yong
A bioelectrochemical sensing system based on a novel whole-cell redox reactivation/cycling module for ultrasensitive detection of pyocyanin (the biomarker of Pseudomonas aeruginosa infections) was developed. The electroactive bacteria mediated redox reactivation module was constructed using Shewanella oneidensis MR-1 cells as the bioelectro-catalyst and lactate as the electron donor. It could regenerate reductive pyocyanin from its oxidative state, which enabled pyocyanin molecule repeatedly registered by the electrode...
July 5, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28704785/detection-of-ca-2-induced-acetylcholine-released-from-leukemic-t-cells-using-an-amperometric-microfluidic-sensor
#18
Mahmood H Akhtar, Khalil K Hussain, N G Gurudatt, Yoon-Bo Shim
A microfluidic structured-dual electrodes sensor comprising of a pair of screen printed carbon electrodes was fabricated to detect acetylcholine, where one of them was used for an enzyme reaction and another for a detection electrode. The former was coated with gold nanoparticles and the latter with a porous gold layer, followed by electropolymerization of 2, 2:5,2-terthiophene-3-(p-benzoic acid) (pTTBA) on both the electrodes. Then, acetylcholinesterase was covalently attached onto the reaction electrode, and hydrazine and choline oxidase were co-immobilized on the detection electrode...
July 5, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28704783/biomass-derived-heteroatoms-doped-mesoporous-carbon-for-efficient-oxygen-reduction-in-microbial-fuel-cells
#19
Yu Lu, Nengwu Zhu, Fuhua Yin, Tingting Yang, Pingxiao Wu, Zhi Dang, Meilin Liu, Xiaorong Wei
Currently, the development of less expensive, more active and more stable catalysts like heteroatom-doped carbon based non-precious metal materials are highly desired for the cathodic oxygen reduction reaction (ORR) in microbial fuel cells (MFCs). Comparing with heteroatom sources from chemical reagents, biomass is notably inexpensive and abundant, containing more elements which contribute to ORR activity. Herein, we demonstrate an easy operating one-step and low-cost way to synthesize egg-derived heteroatoms-doped mesoporous carbon (EGC) catalysts utilizing egg as the biomass carbon and other elements source (sulphur, phosphorus, boron and iron), and porous g-C3N4 as both template and nitrogen source...
July 5, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28697441/a-novel-molecularly-imprinted-electrochemical-sensor-modified-with-carbon-dots-chitosan-gold-nanoparticles-for-the-determination-of-patulin
#20
Wei Guo, Fuwei Pi, Hongxia Zhang, Jiadi Sun, Yinzhi Zhang, Xiulan Sun
In this paper, molecular imprinting technique was applied to the electrochemical sensor. We used 2-oxindole as dummy template, ρ-Aminothiophenol (ρ-ATP) as functional monomers, combined with the high sensitivity of electrochemical detection, to achieve a specific and efficient detection of patulin in fruit juice. In addition, carbon dots and chitosan were used as the modifying material to improve electron-transfer rate, expand the electroactive surface of glassy carbon electrode and enhance strength of the signal...
July 5, 2017: Biosensors & Bioelectronics
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