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Biosensors & Bioelectronics

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https://www.readbyqxmd.com/read/27923189/sensitive-immunoassay-based-detection-of-vibrio-parahaemolyticus-using-capture-and-labeling-particles-in-a-stationary-liquid-phase-lab-on-a-chip
#1
Byunghee Park, Suk-Jung Choi
In the present study, a method was developed for detection of Vibrio parahaemolyticus based on a stationary liquid phase lab-on-a-chip (SLP LOC). The present SLP LOC comprises a sample chamber, washing chamber, and detection chamber connected by two channels. The method utilizes two types of particles: capture particles (CPs), which are magnetic nanoparticles functionalized with antibody; and labeling particles (LPs), which are silica nanoparticles functionalized with horseradish peroxidase and antibody. Samples were added to the sample chamber with CPs and LPs, forming a CP-bacteria-LP complex, and the complex was transported to the detection chamber containing chromogenic substrate solution...
December 1, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27923191/a-highly-specific-ratiometric-two-photon-fluorescent-probe-to-detect-dipeptidyl-peptidase-iv-in-plasma-and-living-systems
#2
Li-Wei Zou, Ping Wang, Xing-Kai Qian, Lei Feng, Yang Yu, Dan-Dan Wang, Qiang Jin, Jie Hou, Zhi-Hong Liu, Guang-Bo Ge, Ling Yang
In this study, a highly specific ratiometric two-photon fluorescent probe GP-BAN was developed and well-characterized to monitor dipeptidyl peptidase IV in plasma and living systems. GP-BAN was designed on the basis of the catalytic properties and substrate preference of DPP-IV, and it could be readily hydrolyzed upon addition of DPP-IV under physiological conditions. Both reaction phenotyping and inhibition assays demonstrated that GP-BAN displayed good reactivity and high selectivity towards DPP-IV over other human serine hydrolases including FAP, DPP-VIII, and DPP-IX...
November 30, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27923190/a-two-photon-fluorescent-probe-for-biological-cu-%C3%A2-and-ppi-detection-in-aqueous-solution-and-in-vivo
#3
Mengmeng Guo, Pengyu Dong, Yan Feng, Xinguo Xi, Rong Shao, Xiaohe Tian, Buchang Zhang, Manzhou Zhu, Xiangming Meng
The first two-photon fluorescent probe (PC) for selectively detecting biological Cu (Ⅱ) and pyrophosphate (PPi) has been developed based on 7-substituted coumarin in this study. The probe presented excellent selective two-photon "on-off-on" detection signal for Cu(II) /PPi in aqueous solution. The two-photon detection ensemble (PCCu) can detect PPi released from DNA amplification after the polymerase chain reactions (PCR). The probe showed low cytotoxicity and good biocompatibility, and therefore can be applied for imaging Cu(II)/PPi in living cells under two-photon excitation...
November 30, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27916536/a-new-lateral-flow-strip-assay-lfsa-using-a-pair-of-aptamers-for-the-detection-of-vaspin
#4
Nurul Hanun Ahmad Raston, Van-Thuan Nguyen, Man Bock Gu
A new lateral flow strip assay (LFSA) using a pair of aptamers has been designed and successfully developed using a pair of aptamers-functionalized with gold nanoparticles (AuNPs). This new LFSA biosensor system utilizes a cognate aptamer duo binding to vaspin, a target protein, at the two different binding sites, and exhibited a sensitive and highly selective response to vaspin. A sandwich-type format in LFSA was developed based on biotin-labeled primary V1 aptamer immobilized on streptavidin coated membrane as a capturing probe and secondary V49 aptamer conjugated with AuNPs as a signaling probe...
November 28, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27918963/isolation-of-a-facultative-anaerobic-exoelectrogenic-strain-lz-1-and-probing-electron-transfer-mechanism-in-situ-by-linking-uv-vis-spectroscopy-and-electrochemistry
#5
Lei Zhou, Dandan Deng, Yichi Zhang, Wen Zhou, Yujing Jiang, Ying Liu
A new facultative anaerobic exoelectrogenic strain LZ-1, belonging to Citrobacter freundii, has been isolated. This strain can produce current densities of 843.9 and 865.6μAcm(-2) using citrate or acetate as carbon source in a three-electrode configuration. The electricity generation performance was also analyzed in a dual-chamber MFC system, reaching a maximum power density of 1233mWm(-2). In addition to acetate and citrate, other carbon sources such as pyruvate, formate, acetate, citrate and fumarate could also be utilized to produce current by strain LZ-1...
November 27, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27914369/dna-tetrahedral-scaffolds-based-platform-for-the-construction-of-electrochemiluminescence-biosensor
#6
Qiu-Mei Feng, Zhen Zhou, Mei-Xing Li, Wei Zhao, Jing-Juan Xu, Hong-Yuan Chen
Proximal metallic nanoparticles (NPs) could quench the electrochemiluminescence (ECL) emission of semiconductor quantum dots (QDs) due to Förster energy transfer (FRET), but at a certain distance, the coupling of light-emission with surface plasmon resonance (SPR) result in enhanced ECL. Thus, the modification strategies and distances control between QDs and metallic NPs are critical for the ECL intensity of QDs. In this strategy, a SPR enhanced ECL sensor based on DNA tetrahedral scaffolds modified platform was reported for the detection of telomerase activity...
November 27, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27914368/highly-sensitive-fluorescence-detection-of-heparin-based-on-aggregation-induced-emission-of-a-tetraphenylethene-derivative
#7
Jiao Zheng, Tai Ye, Jinyang Chen, Li Xu, Xinghu Ji, Chuluo Yang, Zhike He
Tetraphenylethene derivatives were reported for sensitive "turn on" detection of heparin (Hep) based on the typical aggregation-induced emission (AIE). In the present strategy, three derivatives of tetraphenylethene (TPE) were designed,synthesized and compared the sensitivities for the detection of Hep. Furthermore, the (Z)-4,4'-(((1,2-diphenylethene-1,2-diyl) bis(4,1-phenylene))bis (oxy))bis(butan-1-aminium) (Z-TPE-5) was the most sensitive and chosen as the probe for Hep detection. Since Hep could be employed as a medium for inducing the aggregation of positively charged Z-TPE-5, the designed Z-TPE-5 shows excellent fluorescence response to Hep through multiple electrostatic interactions with a detection limit of 1...
November 26, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27914367/single-wall-carbon-nanotube-electrode-system-capable-of-quantitative-detection-of-cd4-t-cells
#8
Joonhyub Kim, Gayoung Park, Seoho Lee, Suk-Won Hwang, Namki Min, Kyung-Mi Lee
Development of CNT-based CD4(+) T cell imunosensors remains in its infancy due to the poor immobilization efficiency, lack of reproducibility, and difficulty in providing linear quantification. Here, we developed a fully-integrated single wall carbon nanotube (SWCNT)-based immunosensor capable of selective capture and linear quantification of CD4(+) T cells with greater dynamic range. By employing repeated two-step oxygen (O2) plasma treatment processes with 35 days of recovery periods, we achieved the enhanced functionalization of the CNT surface and the removal of the byproduct of spray-coated SWCNTs that hinders charge transfer and stable CD4(+) T cell sensing...
November 25, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27907873/excellent-storage-stability-and-sensitive-detection-of-neurotoxin-quinolinic-acid
#9
Ranjana Singh, Sunayana Kashyap, Suveen Kumar, Shiju Abraham, Tejendra K Gupta, Arvind M Kayastha, Bansi D Malhotra, Preeti Suman Saxena, Anchal Srivastava, Ranjan K Singh
Quinolinic acid (QA) is a metabolite of tryptophan degradation obtained through kynurenine pathway, produced naturally in the mammalian brain as well as in the human cerebrospinal fluid. The presence of QA ~10-40µM is a clear indicator of many neurological disorders as well as deficiency of vitamin B6 in human being. In the present work; rapid, sensitive and cost-effective bio-electrodes were prepared to detect the trace amount of endogenous neurotoxin (QA). Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) studies were carried out to measure the electrochemical response of the fabricated bio-electrodes as a function of QA concentrations...
November 23, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27898376/impedimetric-monitoring-of-apoptosis-using-cytochrome-aptamer-bioconjugated-silver-nanocluster
#10
Mojtaba Shamsipur, Afshin Pashabadi, Fatemeh Molaabasi, Saman Hosseinkhani
It is well known that cytochrome c (Cyt c) is a crucial death regulator that triggers programmed cell death, apoptosis. Here, we report on the successful application of an aptamer bioconjugated nanoclusters for the detection of apoptosis based on release of cytochrome c from mitochondria in lysates human embryonic kidney cells HEK293T. The aptamer-conjugated silver nanoclusters (Apt@AgNCs) were synthesized and immobilized on gold electrode for impedimetric detection of Cyt c over the range of 0.15-375nM. It was found that the presence of Ag(I) ions shell at the surface of prepared NCs could increase their affinity to the donor groups (COO(-) and some amine groups in its deprotonated form, NH2) of cysteine attached on the gold electrode surface...
November 23, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27894035/an-isothermal-label-free-and-rapid-one-step-rna-amplification-detection-assay-for-diagnosis-of-respiratory-viral-infections
#11
Bonhan Koo, Choong Eun Jin, Tae Yoon Lee, Jeong Hoon Lee, Mi Kyoung Park, Heungsup Sung, Se Yoon Park, Hyun Jung Lee, Sun Mi Kim, Ji Yeun Kim, Sung-Han Kim, Yong Shin
Recently, RNA viral infections caused by respiratory viruses, such as influenza, parainfluenza, respiratory syncytial virus, coronavirus, and Middle East respiratory syndrome-coronavirus (MERS-CoV), and Zika virus, are a major public health threats in the world. Although myriads of diagnostic methods based on RNA amplification have been developed in the last decades, they continue to lack speed, sensitivity, and specificity for clinical use. A rapid and accurate diagnostic method is needed for appropriate control, including isolation and treatment of the patients...
November 23, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27899266/dna-aptamers-against-foki-nuclease-domain-for-genome-editing-applications
#12
Maui Nishio, Daisuke Matsumoto, Yoshio Kato, Koichi Abe, Jinhee Lee, Kaori Tsukakoshi, Ayana Yamagishi, Chikashi Nakamura, Kazunori Ikebukuro
Genome editing with site-specific nucleases (SSNs) can modify only the target gene and may be effective for gene therapy. The main limitation of genome editing for clinical use is off-target effects; excess SSNs in the cells and their longevity can contribute to off-target effects. Therefore, a controlled delivery system for SSNs is necessary. FokI nuclease domain (FokI) is a common DNA cleavage domain in zinc finger nuclease (ZFN) and transcription activator-like effector nuclease. Previously, we reported a zinc finger protein delivery system that combined aptamer-fused, double-strand oligonucleotides and nanoneedles...
November 22, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27914366/rapid-and-low-cost-biosensor-for-the-detection-of-staphylococcus-aureus
#13
Ghadeer A R Y Suaifan, Sahar Alhogail, Mohammed Zourob
Staphylococcus aureus (S. aureus) is one of the most common etiological agents in hospital-acquired infections and food-borne illness. S. aureus toxins and virulence proteases often circulate in host blood vessels leading to life-threatening diseases. Standard identification approaches include bacterial culturing method, which takes several days. Other nucleic acid-based methods were expensive and required trained personnel. To surmount these limitations, a paper-based biosensor was developed. The sensing mechanism was based on the proteolytic activity of S...
November 21, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27888684/ultrasensitive-non-enzymatic-multiple-immunosensor-for-tumor-markers-detection-by-coupling-dna-hybridization-chain-reaction-with-intercalated-molecules
#14
Jinjin Guo, Junchun Wang, Junjun Zhang, Wenjuan Zhang, Yuzhong Zhang
In this study, we tried coupling the small signal molecules that could intercalate into DNA double helix with hybridization chain reaction (HCR) technique to fabricate a multiple immunosensor. Doxorubicin hydrochloride (DXH) and methylene blue (MB) were used as signal molecules and alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA) were selected as model biomarkers. The immunosensor mainly consists of three parts as follows: First, two different primary antibodies (Ab1) immobilized on the surface of gold nanoparticles (Au NPs); Second, secondary antibodies (Ab2) conjugated with DNA primer; Third, long DNA concatemers from HCR were used as a carrier to intercalate amounts of signal molecules (DXH or MB)...
November 21, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27886600/a-lifetime-sensitive-fluorescence-anisotropy-probe-for-dna-based-bioassays-the-case-of-sybr-green
#15
Benoit Chovelon, Emmanuelle Fiore, Patrice Faure, Eric Peyrin, Corinne Ravelet
In standard steady-state fluorescence anisotropy (FA) DNA-based assays, the ligand binding to a given receptor is typically signalled by the rotational correlation time changes of the tracer. Herein, we report a radically different strategy that relies on the peculiar excited state lifetime features of the SYBR Green (SG) dye. This DNA-binding probe exhibits a drastically short lifetime in solution, leading to a high FA signal. Its complexation to oligonucleotides determines a singular and very large depolarization depending on the concerted effects of extreme lifetime enhancement and resonance energy homotransfer...
November 21, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27888686/integration-of-isothermal-amplification-methods-in-microfluidic-devices-recent-advances
#16
REVIEW
Maria Chiara Giuffrida, Giuseppe Spoto
The integration of nucleic acids detection assays in microfluidic devices represents a highly promising approach for the development of convenient, cheap and efficient diagnostic tools for clinical, food safety and environmental monitoring applications. Such tools are expected to operate at the point-of-care and in resource-limited settings. The amplification of the target nucleic acid sequence represents a key step for the development of sensitive detection protocols. The integration in microfluidic devices of the most popular technology for nucleic acids amplifications, polymerase chain reaction (PCR), is significantly limited by the thermal cycling needed to obtain the target sequence amplification...
November 19, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27886601/one-step-separation-free-detection-of-carcinoembryonic-antigen-in-whole-serum-combination-of-two-photon-excitation-fluorescence-and-optical-trapping
#17
Cheng-Yu Li, Di Cao, Chu-Bo Qi, Hong-Lei Chen, Ya-Tao Wan, Yi Lin, Zhi-Ling Zhang, Dai-Wen Pang, Hong-Wu Tang
Direct analysis of biomolecules in complex biological samples remains a major challenge for fluorescence-based approaches due to the interference of background signals. Herein, we report an analytical methodology by exploiting a single low-cost near-infrared sub-nanosecond pulse laser to synchronously actualize optical trapping and two-photon excitation fluorescence for senstive detection of carcinoembryonic antigen (CEA) in buffer solution and human whole serum with no separation steps. The assay is performed by simultaneously trapping and exciting the same immune-conjugated microsphere fabricated with a sandwich immunization strategy...
November 19, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27886597/paper-based-enzymatic-electrode-with-enhanced-potentiometric-response-for-monitoring-glucose-in-biological-fluids
#18
Marc Parrilla, Rocío Cánovas, Francisco J Andrade
A novel paper-based potentiometric sensor with an enhanced response for the detection of glucose in biological fluids is presented. The electrode consists on platinum sputtered on a filter paper and a Nafion membrane to immobilize the enzyme glucose oxidase. The response obtained is proportional to the logarithm of the concentration of glucose, with a sensitivity of -119±8mV·decade(-1), a linear range that spans from 10(-4)M to 10(-2.5) M and a limit of detection of 10(-4.5) M of glucose. It is shown that Nafion increases the sensitivity of the technique while minimizing interferences...
November 18, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27886603/mno2-nanosheets-as-an-artificial-enzyme-to-mimic-oxidase-for-rapid-and-sensitive-detection-of-glutathione
#19
Jing Liu, Lingjie Meng, Zhaofu Fei, Paul J Dyson, Xunan Jing, Xing Liu
Nanozymes are increasingly used as components in assays and diagnostics. Here, we describe a rapid and highly sensitive colorimetric assay for the detection and quantification of glutathione (GSH) employing MnO2 nanosheets as an artificial oxidase. In the assay pale yellow 3,3´,5,5´-tetramethylbenzidine (TMB) is oxidized to a blue product (oxTMB) under catalyzing of MnO2 nanosheets with a significant change in absorption at 650nm. GSH selectively inhibits this reaction with a detection limit of 300nM. The high specificity of inhibition by GSH allows this system to be used to determine the GSH concentrations in human serum samples...
November 17, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27886599/recent-advances-in-dna-based-electrochemical-biosensors-for-heavy-metal-ion-detection-a-review
#20
REVIEW
M R Saidur, A R Abdul Aziz, W J Basirun
The presence of heavy metal in food chains due to the rapid industrialization poses a serious threat on the environment. Therefore, detection and monitoring of heavy metals contamination are gaining more attention nowadays. However, the current analytical methods (based on spectroscopy) for the detection of heavy metal contamination are often very expensive, tedious and can only be handled by trained personnel. DNA biosensors, which are based on electrochemical transduction, is a sensitive but inexpensive method of detection...
November 16, 2016: Biosensors & Bioelectronics
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