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Biosensors & Bioelectronics

S Dobroiu, F C M J M van Delft, J Aveyard-Hanson, Prasad Shetty, D V Nicolau
Continuous improvements of the fluorescence-based sensitivity and specificity, required for high throughput screening, diagnostics, and molecular biology studies, are usually addressed by better readout systems, or better reporting elements. However, while Fluorescence Interference Contrast (FLIC), which modulates the fluorescence by materials-based parameters, has been used for decades to measure biomolecular interactions at nanometer-precision, e.g., for the study of molecular motors and membrane processes, it has been seldom used for high throughput or diagnostic microdevices...
September 7, 2018: Biosensors & Bioelectronics
Zihua Wang, Yuan Chen, Wenhao Dong, Jiexin Zhou, Bingkai Han, Jun Jiao, Lintao Lan, Peng Miao, Qiang Chen
In this work, carboxylated multi walled carbon nanotubes (CMWCNTs) were firstly prepared and functionalized with poly-L-histidine (PLH), which were then chelated with copper (II) ions to from the nanocomposites of Cu(II)-PLH-CMWCNTs. The nanocomposites could be exploited as an efficient mimic enzyme for sensitive electrochemical detection of salvianic acid A (SAA). Cu(II)-PLH-CMWCNTs owned good charge transfer property and excellent synergetic catalytic effect between the overoxidized imidazole groups and the copper redox-active units...
September 7, 2018: Biosensors & Bioelectronics
Kuan-Wei Li, Yi-Kuang Yen
We developed a Complementary Metal-Oxide-Semiconductor Bio-Microelectromechanical Systems (CMOS-BioMEMS) based piezoresistive microcantilever sensor for detecting gentamicin, a peritonitis therapeutic small-molecule drug. In recent years, the patient-centric concept has been emphasized. In such a trend, therapeutic drug monitoring (TDM) is especially crucial for patients with peritonitis to avoid adverse reactions from a high concentration of gentamicin in the blood. With the aid of a commercialized semiconductor manufacturing process, the microcantilever sensing platform can serve as a portable, low-cost device and offer real-time detection...
September 7, 2018: Biosensors & Bioelectronics
Zili Wang, Xinya Jiang, Ruo Yuan, Yaqin Chai
Herein, N-(4-aminobutyl)-N-(ethylisoluminol) functionalized Fe-based metal-organic frameworks (ABEI/MIL-101(Fe)) with intrinsic mimic peroxidase activity was synthesized and utilized as highly efficient ECL indicator to construct sensitive immunosensor for mucin1 (MUC1) detection. Firstly, compared with the traditional method for ABEI immobilization, the proposed strategy could successfully achieve the highly efficient ABEI immobilization as ABEI coupled 2-aminoterephhalic acid was used as organic bridge ligand...
September 7, 2018: Biosensors & Bioelectronics
Xinan Huang, Huipeng Zhou, Yumeng Huang, Hong Jiang, Na Yang, Sohail Anjum Shahzad, Lianjie Meng, Cong Yu
In this work, we report a novel sensor for colorimetric and fluorometric H2 O2 sensing which is based on silver nanoparticles decorated and tetraphenylethene probe doped silica nanoparticles (Ag@TPE-SiO2 NPs). A positively charged tetraphenylethene (TPE) probe is doped into silica nanoparticles, and the nanoparticles exhibit strong fluorescence emission due to aggregation-induced emission (AIE) of the TPE probe. Ag nanoparticles (AgNPs) are prepared in situ on the surface of the silica nanoparticles. AgNPs serve as a nanoquencher which can quench the AIE emission of the TPE-SiO2 NPs efficiently...
September 7, 2018: Biosensors & Bioelectronics
Dipali R Bagal-Kestwal, M H Pan, Been-Huang Chiang
Enzymes are highly specific and selective due to their precise, intricate three-dimensional catalytic- structure. Electron transfer in enzymes normally occurs through an active-metal centers or tunneling events that are highly insulated by the surrounding globular protein structure. In case of electrochemically active enzymes/proteins, the distance between the redox-active cofactor and the electrode surface plays key role during direct communication. Therefore, the long electron-tunneling distance can be overcome by introducing mobile redox mediators such as nanostructures specially nanowires which can diffuse into and out of the enzyme active site, ferrying reducing or oxidizing equivalents with them...
September 6, 2018: Biosensors & Bioelectronics
Wooseok Choi, Joonchul Shin, Kyung-A Hyun, Jaewoo Song, Hyo-Il Jung
The estimation of bloodstain age is an important factor in forensic analysis. Previously, we have reported a smartphone-based colorimetric system for age estimation of bloodstain, in which Whole blood and EDTA whole blood were dropped on 4 different materials (700 μL) and captured using a smartphone for 72 h. In order to enhance sensitivity and accuracy of the previous system, the current work is dedicated towards the application of pattern recognition and classification of bloodstain images based on a smartphone...
September 5, 2018: Biosensors & Bioelectronics
Jingying Li, Tong Liu, Shuya Liu, Juan Li, Guoming Huang, Huang-Hao Yang
Cholesterol is an essential compound for maintaining cellular homeostasis and human healthy. Sensitive detection of cholesterol and efficient elimination of excess cholesterol have become the essential manipulations in clinical diagnosis and health management. To date, it is still quite challenging that cholesterol detection and elimination tasks are carried out simultaneously. In this study, bifunctional magnetic nanoparticles (Fe3 O4 @PDA-PBA-CD) are designed and fabricated to overcome this difficulty. Taking advantages of competitive host-guest interaction and magnetic separation, highly efficient, reusable and simultaneous cholesterol detection and elimination can be achieved...
September 5, 2018: Biosensors & Bioelectronics
Elisa Michelini, Maria Maddalena Calabretta, Luca Cevenini, Antonia Lopreside, Tara Southworth, Danielle M Fontaine, Patrizia Simoni, Bruce R Branchini, Aldo Roda
Whole-cell biosensors present many advantages, including being able to monitor the toxicity and bioavailability of chemicals; cells grown in traditional 2D cultures, however, do not reproduce the complexity of in vivo physiology. In the last years, 3D cell-culture models have garnered great attention due to their capability to better mimic in vivo cellular responses to external stimuli, providing excellent model living organisms. In order to obtain a predictive, sensitive, and robust yet low-cost 3D cell biosensor, we developed a smartphone-based bioluminescent 3D cell biosensor platform for effect-based analysis...
September 4, 2018: Biosensors & Bioelectronics
Anupriya Baranwal, Pranjal Chandra
Monoamine neurotransmitters (MNTs) belong to one of the most important groups of neurotransmitters in the central nervous system. They play crucial role in functioning of cardiovascular, renal, and hormonal systems along with establishing human brain-body integration. Abnormal level of MNT is associated with numerous psychotic (schizophrenia, depression, dementia, etc.) and neurodegenerative diseases (Alzheimer's, Parkinson's, Huntington's disease, etc.), therefore their sensitive and robust detection is of great clinical significance...
September 3, 2018: Biosensors & Bioelectronics
Tommi Palomäki, Emilia Peltola, Sami Sainio, Niklas Wester, Olli Pitkänen, Krisztian Kordas, Jari Koskinen, Tomi Laurila
No abstract text is available yet for this article.
September 3, 2018: Biosensors & Bioelectronics
Ngoc-Viet Nguyen, Chun-Ping Jen
Enrichment and detection of rare cells are essential in many biological and medical applications. In this study, circulating tumor cells were detected by combining dielectrophoretic (DEP) manipulation and impedance measurement using circular microelectrodes within a single microfluidic device. A prototype of the device was fabricated through standard soft-lithography. With the proposed microchip, target cells (lung cancer cells, A549) were guided toward the center of the working region due to the action of positive DEP and hydrodynamic drag forces and were then trapped onto the desired sensing electrodes...
September 3, 2018: Biosensors & Bioelectronics
Oihane Mitxelena-Iribarren, Jon Zabalo, Sergio Arana, Maite Mujika
Development of new targeted therapies is a challenge in the battle against cancer. Although a variety of treatments is currently available, there is no technique for rapidly evaluating the response of cancer patients to the drug. In this work, a microfluidic platform for the real-time simultaneous analysis of the success rate of different nanoparticle based chemotherapeutic drugs is presented. Based on a previous planar chamber and a reported sensitivity enhancing strategy, linear and cross shape microstructures were integrated into the chamber dome of the microfluidic polydimethylsiloxane and glass platform in order to provide a higher fluid mixing and treatment-cell interaction...
September 2, 2018: Biosensors & Bioelectronics
Elif Burcu Aydın, Muhammet Aydın, Mustafa Kemal Sezgintürk
In this study, we fabricated a label-free electrochemical immunosensor for sensitive and selective detection of tumor marker p53. This immunosensor was based on chitosan/carbon black composite (Chitosan-CB) layer coated ITO electrode. This composite was utilized for enhancement of the conductivity of the immunosensor. Anti-p53 antibodies were captured on the modified ITO electrode through the cross-linking of chitosan and glutaraldehyde. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques were utilized for electrochemical characterization of the proposed immunosensor...
September 2, 2018: Biosensors & Bioelectronics
Xiaofeng Yang, Xiaojun Qin, Yexin Li, Mei Yan, Yu Cui, Guoxin Sun
The detection of Hg2+ in biological systems and its imaging is of highly importance. In this work, a novel ratiometric fluorescence probe is developed based on through-bond energy transfer (TBET) with a 2-(2-hydroxyphenyl)benzoxazole (HBO) as donor and a Rhodamine derivative-Hg conjugate (RDM-Hg) as acceptor. Hg2+ weakens the fluorescence of HBO at 430 nm and meanwhile interacts with Rhodamine B derivative to form a fluorescent conjugate (RDM-Hg) giving rise to emission at 597 nm with a 167 nm red-shift...
September 2, 2018: Biosensors & Bioelectronics
Pan Gao, Hai Wang, Pengwei Li, Wenkai Gao, Yu Zhang, Junli Chen, Nengqin Jia
In this work, a photoelectrochemical (PEC) sensor based on inorganic surface molecular imprinting Nb2 O5 (MI-Nb2 O5 ) for detection of bisphenol A (BPA) had been developed. In the PEC sensor, MI-Nb2 O5 material was synthesized based on an in-situ surface molecular imprinting technique. The microstructure characteristics of the as-prepared photoactive materials were systematically investigated by XRD, SEM, TEM, XPS, FTIR and UV-vis spectroscopy. The PEC detection results showed that the MI-Nb2 O5 material had higher photocurrent responses and excellent selectivity for contaminant BPA under UV-light irradiation owing to the abundant special recognition sites on the surface of MI-Nb2 O5 ...
September 1, 2018: Biosensors & Bioelectronics
Yunpei Si, Jeong Won Park, Sunhee Jung, Geum-Sook Hwang, Eunseo Goh, Hye Jin Lee
A selective biosensing platform for the determination of hypoxanthine (Hx) and uric acid (UA) concentrations in both buffer and human serum sample solutions was developed. The biosensor features the layer-by-layer (LbL) self-assembly of negatively charged xanthine oxidase (XOD) and positively charged poly(diallyldimethyl ammonium chloride) (PDDA) wrapped oxidized multi-walled carbon nanotubes and graphene (CNTs-G) complexes (PDDA-CNTs-G) on screen printed carbon electrode (SPCE) surfaces. Catalytic responses of the XOD modified biosensor with the chosen optimum number of layers for LbL assembly on SPCE towards Hx in buffer solutions were first investigated using both cyclic and square wave voltammetries...
August 31, 2018: Biosensors & Bioelectronics
Saurabh Kumar, Yongjiu Lei, Niman H Alshareef, M A Quevedo-Lopez, Khaled N Salama
In this work, ultrathin Ti3 C2 -MXene nanosheets were synthesized by minimally intensive layer delamination methods, and uniformly functionalized with aminosilane (f-Ti3 C2 -MXene) to provide a covalent binding for the immobilized bio-receptor (anti-CEA) for label free, ultrasensitive detection of cancer biomarker (carcinoembryonic antigen, CEA). The effect of different redox probes on the electrochemical behavior of f-Ti3 C2 -MXene was investigated and found that hexaammineruthenium ([Ru(NH3 )6 ]3+ ) is the preferable redox probe for biosensing...
August 31, 2018: Biosensors & Bioelectronics
Chengmei Liao, Jiali Wu, Lean Zhou, Tian Li, Jingkun An, Zongliang Huang, Nan Li, Xin Wang
Cathodic oxygen reduction catalyzed by autotrophic bacteria instead of a precious metal is a promising method to make use of microbial fuel cells (MFCs) in wastewater treatment with electricity production. However, the ecology of electrotrophic microbial consortia in wastewater systems that function as the catalyst for cathodic oxygen reduction is complicated and the electron transfer mechanisms are still unknown, which prevents further improvements of the biocathode performance. Enriched by the repeated transfer of a mature electrotrophic microbial consortia to new cathodes over 10 generations in 230 days, the start-up time was shortened from 21...
August 31, 2018: Biosensors & Bioelectronics
Charlotte Hurot, Sophie Brenet, Arnaud Buhot, Emilie Barou, Christine Belloir, Loïc Briand, Yanxia Hou
Nowadays, monitoring of volatile organic compounds (VOCs) is very important in various domains. In this work, we aimed to develop sensitive olfactory biosensors using odorant binding proteins (OBPs) as sensing materials. Three rat OBP3 derivatives with customized binding properties were designed and immobilized on the same chip for the detection of VOCs in solution by surface plasmon resonance imaging (SPRi). We demonstrated that the proteins kept their binding properties after the immobilization under optimized conditions...
August 31, 2018: Biosensors & Bioelectronics
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