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Biosensors & Bioelectronics

Antoine Gosset, Claude Durrieu, Louis Renaud, Anne-Laure Deman, Pauline Barbe, Rémy Bayard, Jean-François Chateaux
A critical need exists to develop rapid, in situ, and real-time tools to monitor the impact of pollution discharge toxicity on aquatic ecosystems. The present paper deals with the development of a novel, simple-to-use, low-cost, portable, and user-friendly algal biosensor. In this study, a complete and autonomous portable fluorimeter was developed to assess the A-chlorophyll fluorescence of microalgae, inserted by capillarity into low-cost and disposable xurography-based microfluidic chips. Three microalgae populations were used to develop the biosensor: Chlorella vulgaris, Pseudokirchneriella subcapitata, and Chlamydomonas reinhardtii...
July 5, 2018: Biosensors & Bioelectronics
Manish Sriram, Bijan P Markhali, Philip R Nicovich, Danielle T Bennett, Peter J Reece, D Brynn Hibbert, Richard D Tilley, Katharina Gaus, S R C Vivekchand, J Justin Gooding
The integration of plasmonic nanoparticles into biosensors has the potential to increase the sensitivity and dynamic range of detection, through the use of single nanoparticle assays. The analysis of the localized surface plasmon resonance (LSPR) of plasmonic nanoparticles has allowed the limit of detection of biosensors to move towards single molecules. However, due to complex equipment or slow analysis times, these technologies have not been implemented for point-of-care detection. Herein, we demonstrate an advancement in LSPR analysis by presenting a technique, which utilizes an inexpensive CMOS-equipped digital camera and a dark-field microscope, that can analyse the λmax of over several thousand gold nanospheres in less than a second, without the use of a spectrometer...
July 5, 2018: Biosensors & Bioelectronics
Saeromi Chung, Jong-Min Moon, Jaekyu Choi, Hyundoo Hwang, Yoon-Bo Shim
A magnetic force assisted electrochemical aptamer-antibody sandwich assay (MESA) was developed for the detection of thrombin as a model protein in serum samples. The MESA using the formation of sandwich complexes on the electrochemical sensor probe for reaction and the removal of unbound bioconjugates from the sensor surface without washing are controlled by a magnetic field. Thrombin was determined by the cathodic currents of a toluidine blue O (TBO) attached with thrombin antibody modified magnetic nanoparticle (MNP) at the sensor surface...
July 5, 2018: Biosensors & Bioelectronics
He Wang, Chenxi Zhou, Xiaolu Sun, Yannan Jian, Qingkun Kong, Kang Cui, Shenguang Ge, Jinghua Yu
A novel dual-mode cytosensor based on polyhedral AuPd alloy nanoparticles (PH-AuPd NPs) and three-dimentional reduced graphene oxide (3D-rGO) was constructed for highly sensitive detection of MCF-7 cells. The 3D-rGO was in situ synthesized on the paper working electrode (PWE) by a pollution-free hydrothermal method, increasing the specific surface area and further facilitating the modification of Au nanoparticles (AuNPs). After modified with AuNPs, the Au@ 3D-rGO/PWE was then functionalized by aptamer H1 to trap MCF-7 cells...
July 4, 2018: Biosensors & Bioelectronics
Dorothee Wasserberg, Xichen Zhang, Christian Breukers, Bridgette J Connell, Ellen Baeten, Dorine van den Blink, Èlia S O L À Benet, Andries C Bloem, Monique Nijhuis, Annemarie M J Wensing, Leon W M M Terstappen, Markus Beck
We demonstrate the fabrication of fully printed microfluidic CD4 counting chips with complete on-chip sample preparation and their applicability as a CD4 counting assay using samples from healthy donors and HIV-infected patients. CD4 counting in low-income and resource-limited point-of-care settings is only practical and affordable, if disposable tests can be fabricated at very low cost and all manual sample preparation is avoided, while operation as well as quantification is fully automated and independent of the skills of the operator...
July 3, 2018: Biosensors & Bioelectronics
Sunil K Arya, Pedro Estrela
A novel fluidic-based electrochemical ELISA platform is descried for estimation of the bladder cancer protein markers nuclear mitotic apparatus protein 1 (NUMA1) and complement factor H-related 1 (CFHR1). The platform uses an off-site chamber for a sandwich immunoassay and performs the electrochemistry on-chip in a separate chamber. The off-site matrices were connected to the sensor chip in a manner that the sensors were exposed only to the final electroactive product for signal detection, thus avoiding interference from other molecules present in the sample...
July 3, 2018: Biosensors & Bioelectronics
Kangyao Zhang, Shuzhen Lv, Minghua Lu, Dianping Tang
In this work, a new "signal-on" split-type photoelectrochemical (PEC) sensing platform for prostate-specific antigen (PSA) detection was successfully constructed using p-type Cu-doped Zn0.3 Cd0.7 S as the photosensitive semiconductor material and target-triggered rolling circle amplification (RCA) for signal amplification. The signal derived from Cu-doped Zn0.3 Cd0.7 S was amplified by hemin/G-quadruplex. Upon target PSA introduction, the aptamer-primer probe (apt-pri) was captured by capture antibody-conjugated magnetic bead (MB-mAb) to form the sandwiched MB-mAb/PSA/apt-pri...
July 2, 2018: Biosensors & Bioelectronics
Chen Zhou, Haimin Zou, Chengjun Sun, Dongxia Ren, Jing Chen, Yongxin Li
DNA has well-defined ability to recognize a wide variety of targets, such as small biological molecules, proteins, inorganic ions and small organic molecules. As molecular recognition elements, DNA can be used to build simple, rapid and sensitive biosensors for detection of these targets. DNA-based SPR sensors are considered to be a real-time and label-free tool. We present a systematical and critical review on DNA-based SPR biosensors and their signal amplification via various strategies, focusing on recent advances in nanomaterials, novel DNA amplifications, redox reactions on surface, enzyme amplifications, as well as promising multiplex amplification strategies...
June 30, 2018: Biosensors & Bioelectronics
A T Ezhil Vilian, Ji Yoon Song, Yun Sung Lee, Seung-Kyu Hwang, Hae Jin Kim, Young-Si Jun, Yun Suk Huh, Young-Kyu Han
We report an electrochemical sensor based on three-dimensional porous amorphous carbon (3DPAC) for the sensitive and selective determination of gallic acid (GA). The tailor-made carbon was prepared via salt-templating in which the organic molecular precursor, i.e., glucose, was simply ground and carbonized with a eutectic mixture of LiBr and KBr at 800 °C in an inert atmosphere. Salt removal from the carbon-salt mixture with water yielded 3DPAC with a hierarchical porous structure and oxygen-containing functional groups...
June 30, 2018: Biosensors & Bioelectronics
Michihiko Nakano, Sumit Kalsi, Hywel Morgan
Antimicrobial resistant pathogens are a growing worldwide threat to human health. This study describes a novel method for rapid and sensitive detection of antimicrobial resistance (AMR) genes, specifically blaCTX-M-15 which encodes for the enzyme that offers resistance to extended spectrum β-lactam antibiotics. The method combines isothermal DNA amplification by recombinase polymerase amplification (RPA), with microbead dielectrophoresis (DEP)-based DNA detection. The RPA amplicon is captured onto dielectric microbeads, and the amount of amplicon determined by dielectrophoretic impedance measurement (DEPIM) of the microbeads...
June 30, 2018: Biosensors & Bioelectronics
Ashlesha Bhide, Sriram Muthukumar, Shalini Prasad
Wearable- IOT based low- cost platforms can enable dynamic lifestyle monitoring through enabling promising and exciting opportunities for wellness and chronic- disease management in personalized environments. Diabetic and pre- diabetic populations can modulate their alcohol intake by tracking their glycemic content continuously to prevent health risks through these platforms. We demonstrate the first technological proof of a combinatorial biosensor for continuous, dynamic monitoring of alcohol and glucose in ultra- low volumes (1-5 µL) of passive perspired sweat towards developing a wearable- IOT based platform...
June 30, 2018: Biosensors & Bioelectronics
Shimaa Eissa, Haya Abdulkarim, Majed Dasouki, Hamoud Al Mousa, Rand Arnout, Bandar Al Saud, Anas Abdel Rahman, Mohammed Zourob
Multiplexed biosensors hold great promise for early diagnosis of diseases where the detection of multiple biomarkers is required. Hyper Immunoglobulin E syndromes (HIES) are rare primary immunodeficiency disorders associated with mutations either in the signal transducer and activator of transcription 3 (STAT3), dedicator of cytokinesis 8 DOCK8) or phosphoglucomutase 3 (PGM3) genes. Yet, the diagnosis of HIES is challenged by the complexity of the existing laboratory assays. Here, we report for the first time the development of a multiplexed electrochemical immunosensor for the simultaneous detection of DOCK8, STAT3 and PGM3 proteins...
June 28, 2018: Biosensors & Bioelectronics
Haibin Dong, Fei Zou, Xiaojun Hu, Han Zhu, Kwangnak Koh, Hongxia Chen
Paraquat (PQ) residue is harmful for people's health. This work fabricated an efficient approach to determine PQ sensitively. We exploited a novel surface plasmon resonance (SPR) detection system based on the analyte induced network architecture of supermolecules modified gold nanoparticles (AuNPs) on the chip surface. para-Sulfonatocalix[4]arene (pSC4 ) were used as a recognition molecule for paraquat. PQ can mediate the aggregation of pSC4 capped AuNPs (pSC4 -AuNPs) through the host-guest recognition, which can be used as signal amplification for PQ assay...
June 28, 2018: Biosensors & Bioelectronics
Elyas Hosseinzadeh, Hadi Ravan, Abbas Mohammadi, Rahim Mohammad-Rezaei, Akram Norouzi, Hossein Hosseinzadeh
With the great advances in DNA nanotechnology, scientists have shown interest in developing dynamic nanostructures for theranostic applications, analyte sensing and cargo delivery. Here, we present a specific enzyme-free ultrasensitive platform based on a multilayer coupled signal amplification strategy to quantify miR-21 molecule. The biosensor was integrated based on three signal amplification gadgets, namely a translator-mediated catalytic hairpin assembly (CHA), a multilayer DNA concatemer on the surface of gold decorated magnetic nanoparticle (GMNP), and a DNAzyme-mediated catalytic signal amplification...
June 28, 2018: Biosensors & Bioelectronics
Feng Li, Yuhang Dong, Zhikun Zhang, Man Lv, Zhi Wang, Xinhua Ruan, Dayong Yang
The detection of specific nucleic acids is becoming increasingly important in the discovery of genetic diseases and clinical molecular diagnostics. Here we report a DNA nanostructure-based platform which enables a recyclable biointerface for ultra-sensitive detection of nucleic acid. We created a chemically cross-linked branched DNA nanostructure (CCLB-DNA) as the probe DNA to engineer the biointerfaces, thereby increasing probe distance, exposing more DNA probes from the interface into the solution phase, and thus enhancing the signal dramatically...
June 28, 2018: Biosensors & Bioelectronics
Seyed Hamid Jalalian, Mohammad Ramezani, Noor Mohammad Danesh, Mona Alibolandi, Khalil Abnous, Seyed Mohammad Taghdisi
In this study, a novel electrochemical aptasensor was developed for detection of AFM1 aflatoxin M1 (AFM1 ) based on the hairpin-shaped structure of AFM1 aptamer (Apt), gold nanoparticles (AuNPs) and complementary strand of the aptamer (CS). The conformational change of hairpin structure of Apt in the presence and absence of AFM1 and also negatively charged AuNPs allowed detection of AFM1 with high sensitivity and selectivity. In the absence of the AFM1 , the hairpin structure of the Apt was intact. So, a weak peak current was obtained...
June 28, 2018: Biosensors & Bioelectronics
Cuiying Lin, Huixia Zheng, Yaying Huang, Zhuling Chen, Fang Luo, Jian Wang, Longhua Guo, Bin Qiu, Zhenyu Lin, Huanghao Yang
A sensitive and homogeneous electrochemical aptasensor was fabricated for the detection of mucin 1 (MUC1) by combining a well-designed DNA bulge-loop (L-DNA) structure with high-efficient exonuclease I (Exo I)-assisted target recycling amplification strategy. The L-DNA probe was constructed via the hybridization of the MUC1 aptamer and methylene blue (MB) labeled complementary DNA (cDNA) (cDNA-MB) and hence could not diffuse freely to the negatively charged ITO electrode surface due to the strong electrostatic repulsion, so small electrochemical signal was detected...
June 28, 2018: Biosensors & Bioelectronics
Divya Shakti, Lini Mathew, Neelesh Kumar, Chitra Kataria
BACKGROUND: Though many rehabilitative treatments are available for treatment of spasticity, thus the effectiveness of different robo-rehabilitative devices needs to be evaluated through a systematic review. OBJECTIVE: The objective of this study is to focuses on the efficacy of Robot assistive rehabilitation device for the removal of spasticity from the lower limb of Spastic patients. DATA SOURCESSOURCES: PubMed, Web of Sciences, EMBASE (Excerpta Medical database), CDSR (Cochrane database of systematic reviews), Scopus, IEEE Xplore, Wiley online library, MEDLINE (OvidSP), Science Direct, Springer Link were from January 1980 to September 2017 DATA EXTRACTIONEXTRACTION: Seventy-one publications from eleven databases published were selected using keywords Ankle foot, spasticity, robotic rehabilitation, efficacy of robotics and Ankle foot rehabilitation...
June 28, 2018: Biosensors & Bioelectronics
Tingting Wu, Tao Yan, Xue Zhang, Yixuan Feng, Dong Wei, Meng Sun, Bin Du, Qin Wei
A novel photoelectrochemical (PEC) bioassay protocol for diethylstilbestrol (DES) was systematically proposed. The Au/UiO-66(NH2 ) hybrid with countless active sites was fabricated using an in situ reduction strategy and was subsequently decorated with CdS nanoparticles (NPs) by a sequential chemical bath deposition process. The resulting Au/UiO-66(NH2 )/CdS composites were exploited as a sensing matrix for the first time. The excellent photocurrent response of the as-prepared hybrid under visible light is attributed to its improved photo-electric conversion efficiency due to the local surface plasma resonance (LSPR) effect of Au and the matching energy-level structure between UiO-66(NH2 ) and CdS...
June 26, 2018: Biosensors & Bioelectronics
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