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Biosensors & Bioelectronics

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https://www.readbyqxmd.com/read/29783144/analogous-modified-dna-probe-and-immune-competition-method-based-electrochemical-biosensor-for-rna-modification
#1
Tao Dai, Qinli Pu, Yongcan Guo, Chen Zuo, Shulian Bai, Yujun Yang, Dan Yin, Yi Li, Shangchun Sheng, Yiyi Tao, Jie Fang, Wen Yu, Guoming Xie
N6-methyladenosine (m6A), one of the most abundant RNA methylation which is ubiquitous in eukaryotic RNA, plays vital roles in many biological progresses. Therefore, the rapid and accurate quantitative detection of m6A is particularly important for its functional research. Herein, a label-free and highly selective electrochemical immunosensor was developed for the detection of m6A. The method is established on that the anti-m6A-Ab can recognize both m6A-RNA and m6A-DNA. An analogous modified DNA probe (L1) serves as a signal molecule, by competing with m6A-RNA for binding to Abs to broaden the linear range...
May 18, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29783080/development-and-characterization-of-a-field-effect-biosensor-for-the-detection-of-acetoin
#2
Denise Molinnus, Lukas Muschallik, Laura Osorio Gonzalez, Johannes Bongaerts, Torsten Wagner, Thorsten Selmer, Petra Siegert, Michael Keusgen, Michael J Schöning
A capacitive electrolyte-insulator-semiconductor (EIS) field-effect biosensor for acetoin detection has been presented for the first time. The EIS sensor consists of a layer structure of Al/p-Si/SiO2 /Ta2 O5 /enzyme acetoin reductase. The enzyme, also referred to as butane-2,3-diol dehydrogenase from B. clausii DSM 8716T , has been recently characterized. The enzyme catalyzes the (R)-specific reduction of racemic acetoin to (R,R)- and meso-butane-2,3-diol, respectively. Two different enzyme immobilization strategies (cross-linking by using glutaraldehyde and adsorption) have been studied...
May 18, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29793132/ph-responsive-label-assisted-click-chemistry-triggered-sensitivity-amplification-for-ultrasensitive-electrochemical-detection-of-carbohydrate-antigen-24-2
#3
Yun Zheng, Lihua Zhao, Zhanfang Ma
Sensitivity amplification strategy by implementing click chemistry in the construction of biosensing interface can efficiently improve the performance of immunosensor. Herein, we developed a sandwich-type amperometric immunosensor for ultrasensitive detection of carbohydrate antigen 24-2 (CA 242) based on pH responsive label-assisted click chemistry triggered sensitivity amplification strategy. The sensitivity of amperometric immunosensor relies on the current response differences (ΔI) caused by per unit concentration target analyte...
May 15, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29783081/recent-technological-advancements-in-tuberculosis-diagnostics-a-review
#4
REVIEW
Shagun Gupta, Vipan Kakkar
Early diagnosis and on-time effective treatment are indispensable for Tuberculosis (TB) control - a life threatening infectious communicable disease. The conventional techniques for diagnosing TB normally take two to three weeks. This delay in diagnosis and further increase in detection complexity due to the emerging risks of XDR-TB (Extensively drug Resistant-TB) and MDR-TB (Multidrug Resistant-TB) are evoking interest of researchers in the field of developing rapid TB detection techniques such as biosensing and other point-of-care (POC) techniques...
May 11, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29778001/patchy-gold-coated-fe-3-o-4-nanospheres-with-enhanced-catalytic-activity-applied-for-paper-based-bipolar-electrode-electrochemiluminescence-aptasensors
#5
Xin Zhang, Ning Bao, Xiliang Luo, Shou-Nian Ding
In this work, novel multifunctional patchy gold coated Fe3 O4 hybrid nanoparticles (PG-Fe3 O4 NPs) have been successfully synthesized in aqueous medium via a facile adsorption-reduction method. A rational formation mechanism has been proposed by monitoring the morphological evolution. The PG-Fe3 O4 NPs retained the good magnetic property and exhibited excellent catalytical effeciency towards the electrochemical reduction of hydrogen peroxide. Chronoamperometric and amperometric experiments indicated a relatively high catalytic rate constant of 3...
May 10, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29775852/biomimetic-magnetoelectric-nanocrystals-synthesized-by-polymerization-of-heme-as-advanced-nanomaterials-for-biosensing-application
#6
Jeong Eun Hyeon, Da Woon Jeong, Young Jin Ko, Seung Wook Kim, Chulhwan Park, Sung Ok Han
Regardless of the malaria disease risk, the malaria parasite Plasmodium falciparum has an interesting mechanism. During its growth within the red blood cell, toxic free heme is converted into an insoluble crystalline form called the malaria pigment, or hemozoin. In particular, natural hemozoin nanocrystals can provide multiple applications in biosensing fields for health care and diagnosis as similar to artificial metal nanoparticles. In this study, the heme was biologically synthesized and polymerized by Corynebacterium glutamicum and final polymer was applied as a biomimetic conductive biopolymer...
May 10, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29793133/an-ultrasensitive-and-selective-electrochemical-aptasensor-based-on-rgo-mwcnts-chitosan-carbon-quantum-dot-for-the-detection-of-lysozyme
#7
Behzad Rezaei, Hamid Reza Jamei, Ali Asghar Ensafi
An aptamer-based method is described for the electrochemical determination of lysozyme. A glassy carbon electrode was modified with a nanocomposite composed of reduced graphene oxide (rGO), multi-walled carbon nanotubes (MWCNTs), chitosan (CS), and a synthesized carbon quantum dot (CQD) from CS. The composition of the nanocomposite (rGO-MWCNT/CS/CQD) warrants a high surface-to-volume ratio, high conductivity, high stability, and great electrocatalytic activity. This nanocomposite provides a suitable site for better immobilization of aptamers due to the existence of many amino and carboxyl functional groups, and remaining oxygen-related defects properties in rGO...
May 9, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29778003/highly-efficient-electrochemical-determination-of-propylthiouracil-in-urine-samples-after-selective-electromembrane-extraction-by-copper-nanoparticles-decorated-hollow-fibers
#8
Zeinab Tahmasebi, Saied Saeed Hosseiny Davarani, Ali Akbar Asgharinezhad
In this work, a novel, inexpensive and fast strategy was described for selective and effective extraction and determination of propylthiouracil (PTU) with a high polarity (log P = 1.2) based on electromembrane extraction (EME) followed by differential pulse voltammetry (DPV). For this purpose, copper nanoparticles (CuNPs)-decorated hollow fiber was used as the selective membrane for EME of PTU in urine samples. The influential parameters on extraction such as extraction solvent, pH, agitation speed, applied potential and extraction time were systematically investigated...
May 9, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29778002/plasmonic-colorimetric-sensors-based-on-etching-and-growth-of-noble-metal-nanoparticles-strategies-and-applications
#9
REVIEW
Zhiyang Zhang, Han Wang, Zhaopeng Chen, Xiaoyan Wang, Jaebum Choo, Lingxin Chen
Plasmonic colorimetric sensors have emerged as a powerful tool in chemical and biological sensing applications due to the localized surface plasmon resonance (LSPR) extinction in the visible range. Among the plasmonic sensors, the most famous sensing mode is the "aggregation" plasmonic colorimetric sensor which is based on plasmon coupling due to nanoparticle aggregation. Herein, this review focuses on the newly-developing plasmonic colorimetric sensing mode - the etching or the growth of metal nanoparticles induces plasmon changes, namely, "non-aggregation" plasmonic colorimetric sensor...
May 9, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29775855/dual-core-all-fiber-integrated-immunosensor-for-detection-of-protein-antigens
#10
Karol Wysokiński, Dawid Budnicki, Janusz Fidelus, Łukasz Szostkiewicz, Łukasz Ostrowski, Micha Murawski, Marcin Staniszewski, Magdalena Staniszewska, Marek Napierała, Tomasz Nasiłowski
An optical fiber interferometric microprobe for detection of specific proteins is presented in this paper. The microprobe is an all-fiber device, which is based on Michelson interferometer configuration, which allows for detection of protein antigens in an analyzed solution thanks to antibodies immobilized on the sensor surface. The interferometer is made of dual core fiber and has a precisely formed arm length difference, achieved by splicing a fragment of polarization maintaining fiber to one of the cores...
May 9, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29775856/a-simple-ultrasensitive-electrochemical-sensor-for-simultaneous-determination-of-gallic-acid-and-uric-acid-in-human-urine-and-fruit-juices-based-on-zirconia-choline-chloride-gold-nanoparticles-modified-carbon-paste-electrode
#11
Seyed Alireza Shahamirifard, Mehrorang Ghaedi, Zahra Razmi, Shaaker Hajati
The determination of gallic acid (GA) and uric acid (UA) is essential due to their biological properties. Numerous methods have been reported for the analysis of GA and UA in various real samples. However, the development of a simple, rapid and practical sensor still remains a great challenge. Here, a carbon paste electrode (CPE) was modified by nanocomposite containing zirconia nanoparticles (ZrO2 NPs), Choline chloride (ChCl) and gold nanoparticles (AuNPs) to construct ZrO2 -ChCl-AuNPs/CPE as electrochemical sensor for the simultaneous electro-oxidation of GA and UA...
May 8, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29775854/a-wavelength-modulated-localized-surface-plasmon-resonance-lspr-optical-fiber-sensor-for-sensitive-detection-of-mercury-ii-ion-by-gold-nanoparticles-dna-conjugates
#12
Shuo Jia, Chao Bian, Jizhou Sun, Jianhua Tong, Shanhong Xia
The study presented herein investigated an easy preparation, high performance, wavelength-modulated LSPR optical fiber chemosensor coated by gold nanospheres(AuNS) for Hg2+ detection based on thymine-Hg2+ -thymine base pair mismatches and the coupled plasmonic resonance effect.Utilizing electrostatic self-assembly method, the high density and dispersivity monolayer AuNS coated LSPR fiber sensor had the near field refractive index sensitivity up to 2016 nm/RIU. The single-strand probe DNA served as a binding element for free AuNS labelled-target DNA conjugates was attached to the monolayer AuNS by Au-S bond...
May 8, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29775853/an-ultrasensitive-electrochemiluminescence-biosensor-for-detection-of-microrna-by-in-situ-electrochemically-generated-copper-nanoclusters-as-luminophore-and-tio-2-as-coreaction-accelerator
#13
Hongxia Liao, Ying Zhou, Yaqin Chai, Ruo Yuan
Herein, we constructed an ultrasensitive electrochemiluminescence (ECL) biosensor for detecting microRNA-21 (miR-21) based on in-situ generation of copper nanoclusters (Cu NCs) as luminophore and titanium dioxide (TiO2 ) as coreaction accelerator. First, numerous AT-rich double-stranded DNA (dsDNA) was produced from the conversion of a small amount of target miR-21 via the combination of exonuclease III (Exo III)-assisted amplification and hybridization chain reaction (HCR), which could reduce the aggregation-caused self-etching effect of Cu NCs and improve the emitting of Cu NCs...
May 8, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29772383/a-signal-decreased-electrochemical-immunosensor-for-the-sensitive-detection-of-lag-3-protein-based-on-a-hollow-nanobox-mofs-aupt-alloy
#14
Wei Xu, Zhen Qin, Yutong Hao, Qiang He, Shuai Chen, Zhongshuang Zhang, Dan Peng, Haiyan Wen, Jun Chen, Jingfu Qiu, Chaorui Li
In this work, hollow nanobox metal-organic framework (HNM) nanocomposites were synthesised and utilised for the first time in a signal decreased electrochemical immunosensor for the ultrasensitive quantitative determination of lymphocyte activation gene-3 (LAG-3) protein, which is a newly discovered biomarker. With the aid of signal materials, namely, SiO2 -tagged anti-LAG-3 antibody (SiO2 -Ab2 ) and the biotin-streptavidin system, the sensor can achieve signal amplification. Encapsulation of tin dioxide-functionalised reduced graphene oxide (rGO-SnO2 ) and gold and platinum alloys (AuPt alloys) onto the surface of hollow nanobox metal-organic frameworks (MOFs) was performed to prepare rGO-SnO2 /hollow nanobox-MOFs/AuPt alloys (rGO-SnO2 /HNMs/AuPt) as the matrix...
May 8, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29754053/novel-electrochemical-sensing-platform-for-ultrasensitive-detection-of-cardiac-troponin-i-based-on-aptamer-mos-2-nanoconjugates
#15
Xiujuan Qiao, Kunxia Li, Jinqiong Xu, Ni Cheng, Qinglin Sheng, Wei Cao, Tianli Yue, Jianbin Zheng
Cardiac troponin I (cTnI) is a specific and sensitive biomarker for the early diagnosis of acute myocardial infarction and for the subsequent clinical treatments. In this work, novel electrochemical sensing platform for sensing of cTnI based on aptamer-MoS2 nanoconjugates was proposed. For comparison, core-shell Au@SiO2 @Au nanoparticles were also used for sensing of cTnI. The sensing schemes and electrochemical responses of the proposed sensors were investigated by electrochemical impedance spectroscopy (EIS) in 5...
May 4, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29754052/detection-of-parp-1-activity-based-on-hyperbranched-poly-adp-ribose-polymers-responsive-current-in-artificial-nanochannels
#16
Yong Liu, Jiahui Fan, Haitang Yang, Ensheng Xu, Wei Wei, Yuanjian Zhang, Songqin Liu
The cellular enzyme poly ADP (ADP: adenosine diphosphate)-ribose polymerase-1 (PARP-1) plays key roles in DNA repair. Its activity is closely related to various cancer developments. Detection of PARP-1 activity is significant, however, it is relatively difficult since it lacks superiority property that can be used to detect conveniently. PARP-1 lead to the synthesis of hyperbranched poly (ADP-ribose) polymers (PAR) using nicotinamide adenine dinucleotide (NAD+ ) as substrate during DNA damage repairing. In this paper, we found that hyper-branched PAR increased the steric hindrance and reduced the flux of probe ions effectively in anodic aluminum oxide (AAO) nanochannels...
May 4, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29738945/improved-sensitivity-and-limit-of-detection-of-lateral-flow-devices-using-spatial-constrictions-of-the-flow-path
#17
Ioannis N Katis, Peijun J W He, Robert W Eason, Collin L Sones
We report on the use of a laser-direct write (LDW) technique that allows the fabrication of lateral flow devices with enhanced sensitivity and limit of detection. This manufacturing technique comprises the dispensing of a liquid photopolymer at specific regions of a nitrocellulose membrane and its subsequent photopolymerisation to create impermeable walls inside the volume of the membrane. These polymerised structures are intentionally designed to create fluidic channels which are constricted over a specific length that spans the test zone within which the sample interacts with pre-deposited reagents...
May 3, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29753166/double-determination-of-long-noncoding-rnas-from-lung-cancer-via-multi-amplified-electrochemical-genosensor-at-sub-femtomole-level
#18
Xiaoyan Li, Gang Peng, Feng Cui, Qianying Qiu, Xiaojun Chen, He Huang
An ultrasensitive electrochemical genosensor has been fabricated for the double determination of two different specific sequences deduced from the maternally expressed gene3 (MEG3) lncRNA (long noncoding RNA), which was demonstrated by coupling RNase A-aided target recycling with DNA supersandwich-induced signal enhancement, based on a composite interface of graphene-like tungsten disulfide/dendritic gold nanostructures (WS2 /DGN). Firstly, duple target sequences of T1 and T2 were captured by the primer probes of P1 /P2 functionalized Fe3 O4 @C magnetic nanoparticles, via the DNA/RNA hybridization between T1 /T2 and P1 /P2 ...
May 2, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29751200/biosensor-based-selective-detection-of-zika-virus-specific-antibodies-in-infected-individuals
#19
Gustavo Cabral-Miranda, Ana R Cardoso, Luis C S Ferreira, M Goreti F Sales, Martin F Bachmann
Zika virus (ZIKV) recently emerged as a global threat subsequent to its global spread because it induces microencephaly and other brain damages in infants born to infected mothers. Epidemiological monitoring of infection has been hampered by the absence of reliable serological tests capable to distinguish between ZIKV and other Flavivirus infections, in particular Dengue virus (DENV). As both viruses are transmitted by the same mosquito-species, their distributions largely overlap and reliable serological distinction between the viruses is essential...
May 1, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29734034/monitoring-of-early-diagnosis-of-alzheimer-s-disease-using-the-cellular-prion-protein-and-poly-pyrrole-2-carboxylic-acid-modified-electrode
#20
Jieling Qin, Dong Gyu Jo, Misuk Cho, Youngkwan Lee
Amyloid-beta oligomers (AβΟ) are considered to be reliable biomarkers for the diagnosis of Alzheimer' disease (AD), and the cellular prion protein (PrPC ) is identified as a receptor for AβO. We demonstrated a label and antibody-free electrochemical biosensor for the selective detection of AβO using an electrically conductive poly(pyrrole-2-carboxylic acid) (PPyCOOH) linking agent and PrPC receptor. In the fabrication of the biosensor, each step was characterized by electrochemical impedance spectroscopy and cyclic voltammetry...
May 1, 2018: Biosensors & Bioelectronics
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