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American Journal of Reproductive Immunology: AJRI

Dianchao Lin, Qiansheng Huang, Rongfeng Wu, Songjuan Dai, Zhixiong Huang, Lulu Ren, Sijing Huang, Qionghua Chen
PROBLEM: The role of the long non-coding RNA (lncRNA) actin filament associated protein 1 antisense RNA1 (AFAP1-AS1) in the etiology of endometriosis is unknown. METHOD OF STUDY: Expression of epithelial-mesenchymal transition (EMT) markers was quantified using qRT-PCR, immunohistochemistry, and western blotting. The proliferation, migration, and invasion of ectopic endometrial epithelial cells and Ishikawa cells were evaluated by MTT, EdU, wound healing, and transwell assays...
November 30, 2018: American Journal of Reproductive Immunology: AJRI
Nunzia Zanotta, Lorenzo Monasta, Kristina Skerk, Stefania Luppi, Monica Martinelli, Giuseppe Ricci, Manola Comar
PROBLEM: Cytokines have a significant role in the process of embryo implantation, trophoblast growth, and differentiation by modulating the immune and endocrine system. The aim of this study was to investigate the profile of a large set of cytokines in the cervico-vaginal washing of women undergoing IVF, to explore the association of these proteins with a good receptive endometrium. METHOD OF STUDY: A cohort of 155 women scheduled for IVF cycle was recruited. All patients were asymptomatic for genitourinary infections and had been screened for chlamydia, mycoplasma, and other bacterial infections...
November 26, 2018: American Journal of Reproductive Immunology: AJRI
Ya-Wei Wang, Wang-Sheng Wang, Lu-Yao Wang, Yi-Rong Bao, Jiang-Wen Lu, Yi Lu, Chu-Yue Zhang, Wen-Jiao Li, Kang Sun, Hao Ying
PROBLEM: Rupture of fetal membranes is a crucial event at parturition, which is preceded by extensive extracellular matrix (ECM) remodeling. Our recent studies have demonstrated that the human fetal membranes are capable of de novo synthesis of serum amyloid A1 (SAA1), an acute phase protein, and the abundance of SAA1 in the amnion was increased at parturition. However, the exact role of SAA1 in human parturition remains to be established. METHOD OF STUDY: The effects of SAA1 on the abundance of collagenases and lysyl oxidase, the enzyme that cross-links collagens, were investigated in culture primary human amnion fibroblasts and tissue explants with an aim to examine the involvement of SAA1 in the ECM remodeling in the amnion...
November 21, 2018: American Journal of Reproductive Immunology: AJRI
Mayumi Sugiura-Ogasawara, Takeshi Ebara, Yasuyuki Yamada, Naoto Shoji, Taro Matsuki, Hirohisa Kano, Takahiro Kurihara, Toyonori Omori, Motohiro Tomizawa, Maiko Miyata, Michihiro Kamijima, Shinji Saitoh
PROBLEM: Several studies have reported the increased risk of preterm birth, premature rupture of membranes and low birth weight in patients with recurrent pregnancy loss (RPL). There have been a limited number of large population-based studies examining adverse pregnancy and perinatal outcome after RPL. Multiple-imputed analyses (MIA) adjusting for biases due to missing data is also lacking. METHOD OF STUDY: A nationwide population-based birth cohort study known as the "Japan Environment and Children's Study (JECS)" was conducted by the Ministry of the Environment...
November 14, 2018: American Journal of Reproductive Immunology: AJRI
Giovanni Sisti, Mariarosaria Di Tommaso, Sara Paccosi, Astrid Parenti, Giulia Di Rienzo, Dante Campana, Steven S Witkin
PROBLEM: The influence of fetal sex on immune responses in multifetal pregnancies remains incompletely elucidated. The a2 isoform of vacuolar adenosine triphosphatase (a2V) is expressed on the cell membrane of maternal lymphoid cells and contributes to down regulation of pro-inflammatory immune responses during gestation. The association between fetal sex and a2V expression on peripheral blood mononuclear cells (PBMCs) from mothers with twin gestations was assessed. METHOD OF STUDY: Patients in this prospective study were 93 women with twin pregnancies in their mid second or early third trimester- 27 with two male, 30 with two female and 36 with one male and one female fetus...
November 12, 2018: American Journal of Reproductive Immunology: AJRI
Małgorzata Walentowicz-Sadlecka, Konrad Dziobek, Marek Grabiec, Pawel Sadlecki, Pawel Walentowicz, Paweł Mak, Maria Szymankiewicz, Przemko Kwinta, Magdalena Dutsch-Wicherek
PROBLEM: Aberrant expression of HLA-G in various malignancies has been shown to participate in tumor development by suppressing immune regulation within the tumor microenvironment. The detection of HLA-G has reportedly been correlated with certain clinicopathological parameters in several neoplasms. Both the soluble and membranous forms of HLA-G are biologically active, therefore we aimed to evaluate the HLA-G level by Western blot technique. METHOD OF STUDY: The total amount of HLA-G protein was analyzed in the primary tumor in 113 tissue samples derived from patients with endometrial cancer...
November 10, 2018: American Journal of Reproductive Immunology: AJRI
Gerard Agbayani, Kristina Wachholz, Shawn P Murphy, Subash Sad, Lakshmi Krishnan
PROBLEM: IFN-alpha receptor deficiency (IFNAR-/- ) enhances immunity to Listeria monocytogenes (LM) and Salmonella enterica serovar Typhimurium (ST) in the non-pregnant state by inhibiting pathogen-induced immune cell death. However, the roles of IFNAR signaling in modulating immunity to infection during pregnancy are not well-understood. METHOD OF STUDY: WT and IFNAR-/- mice were infected systemically with LM or ST. Bacterial burden in spleen and individual placentas was enumerated at day 3 post-infection...
October 30, 2018: American Journal of Reproductive Immunology: AJRI
Anup K Talukder, Mohammad B Rashid, Toshiro Takedomi, Satoru Moriyasu, Kazuhiko Imakawa, Akio Miyamoto
PROBLEM: Recently, we demonstrated both in vitro and in vivo that an immunological crosstalk between Day-7 embryo and immune cells exists locally in the uterus in cows. The peripheral immune response to early embryos at Day-7 of pregnancy in cows remains largely unknown. Thus, we aimed to investigate the response of peripheral blood immune cells in the presence of multiple Day-7 embryos in the uterus in donor cows of embryo transfer program. METHOD OF STUDY: Superovulated cows were either inseminated (n=13) at 12-18 h post-estrus or remained without inseminations (n=6)...
October 30, 2018: American Journal of Reproductive Immunology: AJRI
Ya Sun, Jun Shao, Feizhou Jiang, Yanqiu Wang, Qin Yan, Na Yu, Jin Zhang, Jie Zhang, Mingqing Li, Yinyan He
PROBLEM: Endometriosis (EM) is a chronic immunoinflammatory disease associated with an abnormal immunotolerant microenvironment. Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population of immature myeloid cells that play a major role in immunosuppression in cancer, inflammation and other diseases. This paper aims to elucidate whether or not MDSCs are involved in regulating this microenvironment in EM and how this regulation occurs. METHOD OF STUDY: Immunochemistry (IHC) and qPCR were conducted to measure CD11b and ARG1 expression in the ectopic endometrium samples from EM patients...
October 30, 2018: American Journal of Reproductive Immunology: AJRI
Ruhul H Choudhury, Caroline E Dunk, Stephen J Lye, Lynda K Harris, John D Aplin, Rebecca L Jones
PROBLEM: During pregnancy, the decidual spiral arterioles (SpAs) that supply maternal blood to the placenta undergo a series of changes to optimise the transfer of nutrients and oxygen to the developing foetus. Recent studies have shown that initiation of SpA transformation coincides with decidual leucocyte infiltration. Leucocytes are known to be a source of matrix metalloproteinases (MMPs); however, the complete profile of MMPs expressed by decidual NK cells (dNK) and macrophages has not been characterised...
September 29, 2018: American Journal of Reproductive Immunology: AJRI
Zuzanna Kunicka, Aleksandra Kurzynska, Anna Szydlowska, Karol Mierzejewski, Iwona Bogacka
PROBLEM: Cytokines, mediators of the immune response, are involved in the regulation of female reproductive processes during the estrous cycle and pregnancy. The present study aimed to investigate the effect of selected peroxisome proliferator-activated receptor gamma (PPARγ) ligands on the expression of nuclear factor kappa B (NF-κB) and selected cytokines, such as interleukin (IL)-1β, -4, -6, -8, -10, and the leukemia inhibitory factor, in the porcine endometrium on days 10-12 and 14-16 of the estrous cycle (mid- and late luteal phase, respectively) or pregnancy (maternal recognition of pregnancy and beginning of implantation, respectively)...
September 28, 2018: American Journal of Reproductive Immunology: AJRI
Silvia D'Ippolito, Fiorella Di Nicuolo, Alfredo Pontecorvi, Matteo Gratta, Giovanni Scambia, Nicoletta Di Simone
In recent years, extended scientific works shed light on the important role played by the endometrium in early pregnancy. This review examines our current knowledge about the delicate balance between microbial and cellular immune agents at endometrial level: All of them might affect endometrial receptivity. In contrast to the classical thinking of human endometrium as a sterile tissue, several recent studies have drawn attention to a resident population of microorganisms, which reaches only a 30% of concordance with those of the cervical-vaginal flora...
December 2018: American Journal of Reproductive Immunology: AJRI
Ankit Shroff, Kudumula Venkata Rami Reddy
PROBLEM: Candida albicans infection induces damage in host cells that results in the activation of cell death pathways such as apoptosis and necrosis. Autophagy acts as a pro-survival mechanism during various infections and has cross talks with apoptosis. The objective of our study was to delineate the effect of autophagy knockdown in human vaginal epithelial cells (VECs) during Candida infection-induced apoptosis. METHOD OF STUDY: We overexpressed wild-type or mutant form of autophagy-related gene 5 (ATG5) in human VECs and determined the levels of autophagy and lysosome marker genes upon C...
December 2018: American Journal of Reproductive Immunology: AJRI
Sandro La Vignera, Rossella Cannarella, Rosita A Condorelli, Aldo E Calogero
No abstract text is available yet for this article.
December 2018: American Journal of Reproductive Immunology: AJRI
Li Wu, Joanne Kwak-Kim, Ran Zhang, Qing Li, Fang Ting Lu, Yu Zhang, Hao Y Wang, Liang Wen Zhong, Yu Sheng Liu
PROBLEM: The role of vitamin D (VD) in IVF outcome and immune parameters has not been elucidated well. METHOD OF STUDY: Women undergoing IVF treatment with GnRH agonist (Agonist) and progestin-primed ovarian stimulation (PPOS) protocols were divided into VD lower (VDL, 25(OH)VD ≤20 ng/mL) and VD higher (VDH, 25(OH)VD >20 ng/mL) groups. Follicular fluid (FF) VD level, IVF outcomes, and peripheral blood immunophenotypes by flow cytometry were analyzed. RESULTS: FF VD levels of the whole subjects were positively correlated with peripheral blood VD level (r = 0...
December 2018: American Journal of Reproductive Immunology: AJRI
Ioannis Bellos, Vasilios Karageorgiou, Dimitrios Kapnias, Konstantina-Eleni Karamanli, Charalampos Siristatidis
Preeclampsia is a multi-system hypertensive disorder of pregnancy, with significant rates of maternal and neonatal morbidity. It represents a major cause of preterm birth, as definitive treatment demands fetal delivery. Although its pathophysiology is complicated, placental hypoxia and endothelial dysfunction constitute established pathogenetic steps of the disease. Inflammation is considered to be a crucial mediator of preeclampsia process, as an imbalance between TH 1, TH 2, and TH 17 immune responses is observed...
December 2018: American Journal of Reproductive Immunology: AJRI
Samantha Sheller-Miller, Lauren Richardson, Laura Martin, Jin Jin, Ramkumar Menon
Oxidative stress (OS) plays a role in uterine tissue remodeling during pregnancy and parturition. While p38 MAPK is an OS-response kinase, a precise functional role is unknown. Therefore, we conducted a systematic review of literature on p38 MAPK expression, activation, and function in reproductive tissues throughout pregnancy and parturition, published between January 1980 and August 2017, using four electronic databases (Web of Science, PubMed, Medline, and CoCHRANE). We identified 418 reports; 108 were selected for full-text evaluation and 74 were included in final review...
December 2018: American Journal of Reproductive Immunology: AJRI
Martha Lappas
PROBLEM: Metallothioneins (MTs) play important roles in regulating oxidative stress, inflammation, and hormone signaling. These processes play a major role in labor at term and preterm. The aims of this study were to characterize (a) temporal- and labor-associated changes and (b) the effect of pro-inflammatory and pro-labor insults on the expression of MT1 isoforms, MT2A, MT3, and MT4 in fetal membranes and myometrium. METHOD OF STUDY: The expression of MTs was assessed in fetal membranes and myometrium from nonlaboring and laboring women at preterm and term by RT-qPCR...
December 2018: American Journal of Reproductive Immunology: AJRI
Dandan Li, Jian Li, Bin Jia, Yue Wang, Juxin Zhang, Guangzhi Liu
PROBLEM: This study revealed miRNA regulation and functional microarray-based profiles of miRNAs in the natural killer (NK) cells of the decidual tissue obtained from patients with unexplained recurrent spontaneous abortion (URSA). METHOD OF STUDY: Patients with URSA were categorized based on the occurrence of at least two or more successive spontaneous abortions between 7th and 10th gestational week. Total RNA was isolated from the NK cells of the decidual tissue obtained from patients with induced abortion at about the 8th gestational week...
November 2018: American Journal of Reproductive Immunology: AJRI
Emeir M McSorley, Alison J Yeates, Maria S Mulhern, Edwin van Wijngaarden, Katherine Grzesik, Sally W Thurston, Toni Spence, William Crowe, Philip W Davidson, Grazyna Zareba, Gary J Myers, Gene E Watson, Conrad F Shamlaye, J J Strain
PROBLEM: Maternal methylmercury (MeHg) exposure may be associated with immune response during pregnancy. METHOD OF STUDY: In the high fish-eating Seychelles Child Development Study Nutrition Cohort 2, we examined the association between maternal MeHg, polyunsaturated fatty acids (PUFA), and immune markers (Th1:Th2; TNF-α, IL-1β, IFN-γ, IL-2, IL-4, IL-5, IL-10, MCP-1, TARC, sFlt-1, VEGF-D, CRP and IL-6) at 28 weeks' gestation. Linear regression examined associations between MeHg exposure and immune markers with and without adjustment for PUFA...
November 2018: American Journal of Reproductive Immunology: AJRI
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