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American Journal of Reproductive Immunology: AJRI

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https://www.readbyqxmd.com/read/29205643/paternal-hla-c-is-a-risk-factor-in-unexplained-recurrent-miscarriage
#1
Tess Meuleman, Geert W Haasnoot, Jan M M van Lith, Willem Verduijn, Kitty W M Bloemenkamp, Frans H J Claas
PROBLEM: HLA-C is the only classical HLA-I antigen expressed on trophoblast. We hypothesized that the alloimmune response to paternal HLA-C plays a role in unexplained recurrent miscarriage. METHOD OF STUDY: In a case-control design, we included 100 women with at least three unexplained consecutive miscarriages along with their partners and children. For the first control group, we included 90 women with an uneventful singleton pregnancy without pregnancy complications in their history along with their children...
December 4, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29205636/immune-checkpoint-molecules-soluble-program-death-ligand-1-and-galectin-9-are-increased-in-pregnancy
#2
Elizabeth Ann L Enninga, Susan M Harrington, Douglas J Creedon, Rodrigo Ruano, Svetomir N Markovic, Haidong Dong, Roxana S Dronca
PROBLEM: Pregnancy requires balance between tolerance to the haploidentical fetus and the mother's ability to mount immune responses. There are parallels to this phenomenon that occur in metastatic cancer. We assessed soluble program death ligand-1 soluble PD-L1 (sPD-L1) and galectin-9 in the blood of pregnant women during gestation as these molecules are highly involved in immune suppression during cancer. METHOD OF STUDY: Maternal blood was collected from 30 primigravida women at monthly intervals during pregnancy, delivery and 6-week post-partum...
December 4, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29205631/maternal-cd8-t-cell-depletion-alleviates-intrauterine-inflammation-induced-perinatal-brain-injury
#3
Jun Lei, Li Xie, Hongxi Zhao, Candice Gard, Julia L Clemens, Michael W McLane, Mia C Feller, Maide Ozen, Christopher Novak, Wael Alshehri, Nader Alhejaily, Yahya Shabi, Jason M Rosenzweig, Andrea Facciabene, Irina Burd
We investigated the mechanisms by which CD8+ T-cell trafficking in placenta contributes to perinatal brain injury by studying effects of maternal CD8+ T-cell depletion (DEP) in a mouse model of intrauterine inflammation (IUI). Maternal CD8+ T cells were depleted with anti-CD8+ antibodies. IUI was induced with lipopolysaccharide (LPS). DEP was confirmed using flow cytometry. Preterm birth rate was evaluated. Offspring neurologic sequelae were assessed by Nissl staining, immune arrays, confirmatory individual TaqMan® gene assays, and neurobehavioral tests...
December 4, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29205625/vitamin-d-facilitates-trophoblast-invasion-through-induction-of-epithelial-mesenchymal-transition
#4
Ryang Hee Kim, Byung Jun Ryu, Ki Mo Lee, Jae Won Han, Sung Ki Lee
PROBLEM: Vitamin D deficiency increases the risk of developing pregnancy-related complications, including preeclampsia and small-for-gestational-age infants. Vitamin D was demonstrated to promote the invasiveness of human extravillous trophoblasts (EVTs). However, whether vitamin D induces the epithelial-mesenchymal transition (EMT) of EVTs remains unclear. Therefore, we investigated whether vitamin D promotes EMT and the related signaling pathways. METHOD OF STUDY: In this study, we performed EMT experiments using JAR cells based on the expression of the mesenchymal markers and vitamin D receptor...
December 4, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29197148/decidual-cd68-hla-dr-cd163-m1-macrophages-increase-in-miscarriages-with-normal-fetal-chromosome
#5
Shigeki Shimada, Yasuhiko Ebina, Norifumi Iijima, Masashi Deguchi, Hideto Yamada
PROBLEM: Is an abnormal increase or decrease of M1/M2 macrophages observed in the deciduae of miscarriages with normal fetal chromosome (MN)? METHODS OF STUDY: Deciduae of 18 MN and 26 miscarriages with abnormal fetal chromosome (MA) were obtained. Additionally, deciduae from 15 women whose pregnancies ended in induced abortions (IA) and endometriums at the mid-luteal phase from 19 non-pregnant women endomeriums of mid-luteal phases (EM) were obtained. Macrophages were analyzed by flow cytometry using monoclonal antibodies for CD68, HLA-DR, and CD163...
December 2, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29194839/analysis-of-the-ectoenzymes-ada-alp-enpp1-and-enpp3-in-the-contents-of-ovarian-endometriomas-as-candidate-biomarkers-of-endometriosis
#6
Carla Trapero, Lluis Jover, Maria Eulàlia Fernández-Montolí, Amparo García-Tejedor, August Vidal, Inmaculada Gómez de Aranda, Jordi Ponce, Xavier Matias-Guiu, Mireia Martín-Satué
PROBLEM: The diagnosis of endometriosis, a prevalent chronic disease with a strong inflammatory component, is usually delayed due to the lack of noninvasive diagnostic tests. Purinergic signaling, a key cell pathway, is altered in many inflammatory disorders. The aim of the present work was to evaluate the levels of adenosine deaminase (ADA), alkaline phosphatase (ALP), ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), and ENPP3, elements of purinergic signaling, as biomarker candidates for endometriosis...
November 30, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29194835/oxidative-stress-diseases-unique-to-the-perinatal-period-a-window-into-the-developing-innate-immune-response
#7
Robert M Dietz, Clyde J Wright
The innate immune system has evolved to play an integral role in the normally developing lung and brain. However, in response to oxidative stress, innate immunity, mediated by specific cellular and molecular programs and signaling, contributes to pathology in these same organ systems. Despite opposing drivers of oxidative stress, namely hyperoxia in neonatal lung injury and hypoxia/ischemia in neonatal brain injury, similar pathways-including toll-like receptors, NFκB and MAPK cascades-have been implicated in tissue damage...
November 30, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29193446/a-distinct-mechanism-of-senescence-activation-in-amnion-epithelial-cells-by-infection-inflammation-and-oxidative-stress
#8
Christopher Luke Dixon, Lauren Richardson, Samantha Sheller-Miller, George Saade, Ramkumar Menon
PROBLEM: We investigated p38MAPK activation-induced fetal membrane cell senescence in response to inflammation (tumour necrosis factor-alpha [TNF-α]) and infection (lipopolysaccharide [LPS]), factors associated with spontaneous preterm birth. METHOD OF STUDY: Primary amnion epithelial cells (AECs) were exposed to TNF-α, 50 ng/mL and LPS, 100 ng/mL. Cigarette smoke extract (CSE), a known OS inducer, was used as positive control. AECs were cotreated with the antioxidant N-acetyl cysteine (NAC) and p38MAPK inhibitor SB203580 to determine the effect of OS and p38MAPK...
November 30, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29193463/placental-exosomes-a-proxy-to-understand-pregnancy-complications
#9
Jin Jin, Ramkumar Menon
Exosomes (30- to 150-nm particles), originating from multivesicular bodies by the invagination of the endosomal membrane, are communication channels between cells. Exosomes are released by various cell types and cargo proteins, lipids, and nucleic acids reflecting the physiologic status of their cells of origin and cause functional changes in recipient cells, which are likely dependent on their quantity and/or cargo contents. Recently, placental exosomes, produced by various placental cell types, have been isolated from maternal blood using the placental protein-specific marker, placental alkaline phosphatase (PLAP)...
November 28, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29193454/amniotic-fluid-pentraxins-potential-early-markers-for-identifying-intra-amniotic-inflammatory-complications-in-preterm-pre-labor-rupture-of-membranes
#10
Ivana Musilova, Ctirad Andrys, Jan Krejsek, Marcela Drahosova, Barbora Zednikova, Lenka Pliskova, Helena Zemlickova, Bo Jacobsson, Marian Kacerovsky
In this study, pentraxin 3 (PTX3), C-reactive protein (CRP), and serum amyloid P component (SAP) concentrations in the amniotic fluid of women with preterm pre-labor rupture of membranes (PPROM) were evaluated based on evidence of microbial invasion of the amniotic cavity (MIAC), intra-amniotic inflammation (IAI), and microbial-associated IAI. A total of 149 women with PPROM were included in this study. Amniotic fluid samples were obtained by transabdominal amniocentesis. Amniotic fluid PTX3, SAP, and CRP concentrations were assessed using enzyme-linked immunosorbent assay...
November 28, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29193441/therapy-for-antiphospholipid-miscarriages-throwing-the-baby-out-with-the-bathwater
#11
Cecilia Beatrice Chighizola, Yehuda Shoenfeld, Pier Luigi Meroni
PROBLEM: The association of low molecular weight heparin (LMWH) with low-dose aspirin (LDASA) provides the therapeutic cornerstone of obstetric anti-phospholipid syndrome (APS). This combo approach is not effective in all patients, and few women still experience recurrences. METHOD OF STUDY: In an elegant in vitro study, Chiombori Quao and colleagues demonstrated that anti-phospholipid antibodies (aPL) affect the functionality of endometrial endothelial cells interfering with angiogenesis...
November 28, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29154465/the-role-of-immunological-testing-and-intervention-in-reproductive-medicine-a-fertile-collaboration
#12
REVIEW
Syed B Ali, Yogesh Jeelall, Craig E Pennell, Roger Hart, Andrew McLean-Tooke, Michaela Lucas
Advances in reproductive medicine have significantly increased the success of fertility treatments. Nevertheless, some women experience recurrent implantation failure (RIF) after in-vitro fertilization (IVF) or recurrent pregnancy loss (RPL). Imbalances in the immune system and failure to achieve immune tolerance to the foetus have been implicated as potentially modifiable causes of idiopathic RIF and RPL. As such, women are increasingly being treated with immunomodulatory agents in an attempt to achieve a successful pregnancy...
November 20, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29154462/the-role-of-indoleamine-2-3-dioxygenase-in-normal-and-pathological-pregnancies
#13
REVIEW
Rui-Qi Chang, Da-Jin Li, Ming-Qing Li
The survival of allogeneic fetus during pregnancy contradicts the laws of immune responses. Behind this paradoxical phenomenon, the mechanism is quite complex. Indoleamine-2,3-dioxygenase (IDO) is the first and rate-limiting enzyme of tryptophan catabolism. Emerging evidence shows that IDO is expressed at the maternal-fetal interface, including trophoblast cells, decidual stroma cells, decidual immune cells (eg, natural killer cells, T cells, and macrophages), and vascular endothelial cells of decidua and chorion...
November 20, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29154408/chemokine-c-c-motif-ligand-25-expressed-by-trophoblast-cells-and-leukocytes-bearing-its-receptor-ccr9-an-alliance-during-embryo-implantation
#14
Rodrigo Barbano Weingrill, Mara S Hoshida, Ciro Dresch Martinhago, Simone Correa-Silva, Elaine Cardoso, Patrícia Palmeira, Claudio Romero Farias Marinho, Estela Bevilacqua
PROBLEM: We hypothesized that trophoblast expression of Ccl25 attracts a specific leukocyte cell population to the implantation site for local regulation. METHOD OF STUDY: Mice blastocysts, ectoplacental cones, and decidua at gestational days 3.5-7.5 were evaluated for Ccl25 and Ccr9 expressions. Peripheral availability and characterization of Ccr9+ leukocytes were determined by flow cytometry. Leukocyte chemotaxis was assessed in the presence of Ccl25 recombinant protein and embryos using antisense oligomers (ODNs) to Ccl25 and Ccr9 neutralizing antibody...
November 20, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29154392/rapid-and-simple-detection-of-ureaplasma-species-from-vaginal-swab-samples-using-a-loop-mediated-isothermal-amplification-method
#15
Kazumasa Fuwa, Mitsuko Seki, Yoshiyasu Hirata, Itaru Yanagihara, Yukiko Nakura, Chika Takano, Kazumichi Kuroda, Satoshi Hayakawa
PROBLEM: Ureaplasma species occasionally cause chorioamnionitis and premature labor. We developed a novel assay employing a loop-mediated isothermal amplification (LAMP) method to detect Ureaplasma parvum and Ureaplasma urealyticum. METHOD OF STUDY: Loop-mediated isothermal amplification primers were designed to amplify Ureaplasma-specific ureaseB genes. Four U. parvum strains, 5 U. urealyticum strains and 14 reference bacterial species were evaluated. Forty-six vaginal swab samples were analyzed by LAMP, culture, and PCR...
November 20, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29135053/chronic-endometritis-in-patients-with-unexplained-infertility-prevalence-and-effects-of-antibiotic-treatment-on-spontaneous-conception
#16
Ettore Cicinelli, Maria Matteo, Giueseppe Trojano, Paola C Mitola, Raffaele Tinelli, Amerigo Vitagliano, Francesco M Crupano, Achiropita Lepera, Giuseppe Miragliotta, Leonardo Resta
PROBLEM: The correlations between chronic endometritis and unexplained infertility are unexplored. METHOD OF STUDY: We performed a retrospective study on consecutive patients referred to our hysteroscopy service due to unexplained infertility. All women underwent endometrial sampling with histological and cultural examinations. If chronic endometritis was diagnosed, patients received antibiotic therapy, and chronic endometritis resolution was subsequently ascertained by histological examination...
November 14, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29135051/low-molecular-weight-heparin-and-aspirin-exacerbate-human-endometrial-endothelial-cell-responses-to-antiphospholipid-antibodies
#17
Zola Chihombori Quao, Mancy Tong, Elena Bryce, Seth Guller, Lawrence W Chamley, Vikki M Abrahams
PROBLEM: Women with antiphospholipid antibodies (aPL) are at risk for pregnancy complications despite treatment with low molecular weight heparin (LMWH) or aspirin (ASA). aPL recognizing beta2 glycoprotein I can target the uterine endothelium, however, little is known about its response to aPL. This study characterized the effect of aPL on human endometrial endothelial cells (HEECs), and the influence of LMWH and ASA. METHOD OF STUDY: HEECs were exposed to aPL or control IgG, with or without low-dose LMWH and ASA, alone or in combination...
November 14, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29131441/genetic-and-epigenetic-regulation-of-major-histocompatibility-complex-class-i-gene-expression-in-bovine-trophoblast-cells
#18
Bi Shi, Aaron J Thomas, Abby D Benninghoff, Benjamin R Sessions, Qinggang Meng, Parveen Parasar, Heloisa M Rutigliano, Kenneth L White, Christopher J Davies
PROBLEM: The regulatory mechanisms governing differential expression of classical major histocompatibility complex (MHC) class I (MHC-Ia) and non-classical MHC class I (MHC-Ib) genes are poorly understood. METHOD OF STUDY: Quantitative reverse transcription- polymerase chain reaction (PCR) was used to compare the abundance of MHC-I transcripts and related transcription factors in peripheral blood mononuclear cells (PBMC) and placental trophoblast cells (PTC). Methylation of MHC-I CpG islands was detected by bisulfite treatment and next-generation sequencing...
November 12, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29105931/medroxyprogesterone-acetate-treated-human-primary-endometrial-epithelial-cells-reveal-unique-gene-expression-signature-linked-to-innate-immunity-and-hiv-1-susceptibility
#19
Matthew W Woods, Muhammad Atif Zahoor, Sara Dizzell, Chris P Verschoor, Charu Kaushic
PROBLEM: Medroxyprogesterone acetate (MPA), a progestin-based hormonal contraceptive designed to mimic progesterone, has been linked to increased human immunodeficiency virus (HIV-1) susceptibility. Genital epithelial cells (GECs) form the mucosal lining of the female genital tract (FGT) and provide the first line of protection against HIV-1. The impact of endogenous sex hormones or MPA on the gene expression profile of GECs has not been comprehensively documented. METHOD OF STUDY: Using microarray analysis, we characterized the transcriptional profile of primary endometrial epithelial cells grown in physiological levels of E2, P4, and MPA...
November 6, 2017: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/29105884/inhibition-of-iap-inhibitor-of-apoptosis-proteins-represses-inflammatory-status-via-nuclear-factor-kappa-b-pathway-in-murine-endometriosis-lesions
#20
Fuminori Taniguchi, Takashi Uegaki, Kazuomi Nakamura, Khine Yin Mon, Takashi Harada, Tetsuya Ohbayashi, Tasuku Harada
PROBLEM: How is the role of inhibitor of apoptosis proteins (IAPs) in the development of murine endometriosis lesions? METHOD OF STUDY: BALB/c female mice (n = 36) were used for the murine endometriosis model. Endometriotic lesions were surgically induced in mice by transplanting mouse uterine tissue. After 4 weeks of IAP antagonist (BV6) treatment, the expression of inflammatory factors in the implants was evaluated using real-time RT-PCR. Inflammatory state, angiogenic activity, and nuclear factor-kappa B (NF-κB) activation were assessed by immunohistochemical staining...
November 6, 2017: American Journal of Reproductive Immunology: AJRI
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