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American Journal of Reproductive Immunology: AJRI

Catarina Martins, Jorge Lima, Glória Nunes, Luís Miguel Borrego
PROBLEM: Maternal atopy is a risk factor for allergy. B cells are poorly studied in reproduction and atopy. We aimed to assess how pregnancy affects B cells in atopic women and whether B cells relate to allergic manifestations in offspring. METHOD OF STUDY: Women with and without atopic asthma, pregnant and non-pregnant were enrolled for the study, and circulating B cells were evaluated by flow cytometry, using CD19, CD27, CD38, IgD, and IgM. RESULTS: Compared to healthy non-pregnant, atopic asthmatic non-pregnant (ANP) women presented increased B cell counts, enlarged memory subsets, less transitional cells, and plasmablasts...
October 25, 2016: American Journal of Reproductive Immunology: AJRI
Hans-Werner Denker
No abstract text is available yet for this article.
October 24, 2016: American Journal of Reproductive Immunology: AJRI
Li Wu, Jie Li, Hui Li Xu, Bo Xu, Xian Hong Tong, Joanne Kwak-Kim, Yu Sheng Liu
PROBLEM: We aim to investigate a possible role of IL-7/IL-7R signaling pathway in recurrent pregnancy losses (RPL). MATERIAL AND METHODS: Using the abortion-prone (AP) and non-abortion-prone (NP) mice model, fetal resorption rates (FRR), Th17 and Treg cells-related factors, and the effect of IL-7 and IL-7R antagonist were investigated by flow cytometry, quantitative real-time PCR, and immunohistochemistry. IL-7 and IL-7R expressions in human decidua were investigated by immunohistochemistry...
October 21, 2016: American Journal of Reproductive Immunology: AJRI
Kaori Yamada-Nomoto, Osamu Yoshino, Ikumi Akiyama, Akemi Ushijima, Yosuke Ono, Tomoko Shima, Akitoshi Nakashima, Shusaku Hayashi, Makoto Kadowaki, Yutaka Osuga, Shigeru Saito
OBJECTIVE: We investigated α-7 nAchR expression in human peritoneal macrophages and examined whether activation of nAchR might be a new therapy for endometriosis. MATERIALS AND METHODS: Human peritoneal fluid mononuclear cells (PFMC) were stimulated with lipopolysaccharide (LPS) in the presence of α-7 nAChR agonists. In a murine endometriosis model, α-7 nAChR modulators were administered. RESULTS: Human PFMC expressed α-7 nAChR at the mRNA and protein levels...
October 21, 2016: American Journal of Reproductive Immunology: AJRI
Yuping Wang, Qin Dong, Yang Gu, Lynn J Groome
PROBLEM: To determine whether miR-203 mediates endothelial inflammatory response in preeclampsia. METHOD OF STUDY: Maternal vessel miR-203 expression was assessed by in situ hybridization. Suppressor of cytokine signaling-3 (SOCS-3) and ICAM expression was determined by immunostaining. Subcutaneous fat tissue sections from normal and preeclamptic pregnant women were used. miR-203-induced inflammatory response was evaluated by the measurements of IL-6, IL-8, ICAM, and VCAM expression and production and neutrophil adhesion in the endothelial cells (EC) transfected with miR-203 precursor, pre-miR-203...
October 18, 2016: American Journal of Reproductive Immunology: AJRI
Fabiana Cro', Cristina Lapucci, Emilio Vicari, Ginevra Salsi, Nicola Rizzo, Antonio Farina
OBJECTIVE: The aim of this study was to present a new method for fetal Kell genotyping by means of the allelic discrimination of K1 and K2 in real-time polymerase chain reaction (PCR). METHODS: Real-time quantitative polymerase chain reaction incorporating an allele-specific primer was developed for detecting the K allele of KEL. RESULTS: By means of this method, the K1/K2 genotype was able to be determined in all blood samples analyzed. Results using cell-free fetal DNA (cffDNA) from two Kell-negative pregnant women confirmed the Kell-positive genotype of fetuses...
October 11, 2016: American Journal of Reproductive Immunology: AJRI
Georgia Fernanda Gelmini, Cynthia Hernandes Costa, Fabiola da Silva Nardi, Pryscilla Fanini Wowk, Sibelle Botogosque Mattar, Alessandro Schuffner, Maria da Graça Bicalho, Valéria Maria Munhoz Sperandio Roxo
BACKGROUND: HLA-E products, class Ib human leukocyte antigens, act in the immunology of human reproduction as modulators of the maternal immune system during pregnancy. AIMS: To evaluate HLA-E role in the establishment of a viable pregnancy. MATERIALS & METHODS: HLA-E was genotyped by sequence-based typing (SBT) and analyzed for specific polymorphisms, comparing couples who underwent assisted reproduction treatment (ART) and fertile control couples...
October 7, 2016: American Journal of Reproductive Immunology: AJRI
Xian Chen, Biao Yin, Ruo-Chun Lian, Tao Zhang, Hong-Zhan Zhang, Liang-Hui Diao, Yu-Ye Li, Chun-Yu Huang, De-Sheng Liang, Yong Zeng
PROBLEM: We aimed to investigate the modulatory effects of vitamin D on peripheral blood cellular immune response in patients with recurrent miscarriage (RM). METHOD OF STUDY: The effect of vitamin D on the number of peripheral blood cells, T helper 1 (Th1) cytokines, and NK cytotoxicity was measured in 99 women with RM. RESULTS: The percentage of CD19(+) B cells and NK cytotoxicity at an effector-to-target cell (E:T) ratio of 50:1, 25:1, and 12...
October 1, 2016: American Journal of Reproductive Immunology: AJRI
Chiara Agostinis, Tamara Stampalija, Dionne Tannetta, Claudia Loganes, Liza Vecchi Brumatti, Francesco De Seta, Claudio Celeghini, Oriano Radillo, Ian Sargent, Francesco Tedesco, Roberta Bulla
PROBLEM: We have previously found that C1q is constitutively expressed by invading trophoblast and endothelial cells of decidua and contributes to vascular and tissue remodeling. Based on these findings, we sought to determine whether there were changes in the circulating level of C1q that may be used as a diagnostic and predictive marker of preeclampsia. METHOD OF STUDY: We measured the levels of C1q, C4, and complement activation products in serum or plasma of normal pregnant women and preeclamptic patients from different cohorts...
September 26, 2016: American Journal of Reproductive Immunology: AJRI
Pankaj Suman, Sonu Gandhi, Prabhanshu Kumar, Kirti Garg
Preeclampsia is a vascular multisystem disorder that accounts for varying degree of morbidity and mortality of mother and the fetus. This can be significantly averted if diagnosed at an early (18-20 weeks) stage of gestation, as there is no known way to prevent preeclampsia. In spite of extensive work on biomarker discovery, the existing method for its detection is mostly based on colorimetric immunoassays whose sensitivity is ranging in nanomolar range. Further, it has also been observed that change in the expression of a single biomarker is not sufficient to diagnose this condition...
September 25, 2016: American Journal of Reproductive Immunology: AJRI
Nardhy Gomez-Lopez, Roberto Romero, Marcia Arenas-Hernandez, Hyunyoung Ahn, Bogdan Panaitescu, Felipe Vadillo-Ortega, Carmen Sanchez-Torres, Katherine S Salisbury, Sonia S Hassan
PROBLEM: Activated/effector T cells seem to play a role in the pathological inflammation associated with preterm labor. The aim of this study was to determine whether in vivo T-cell activation by a monoclonal αCD3ε antibody induces preterm labor and birth. METHOD OF STUDY: Pregnant B6 mice were intraperitoneally injected with a monoclonal αCD3ε antibody or its isotype control. The gestational age, the rates of preterm birth and pup mortality at birth as well as the fetal heart rate and umbilical artery pulsatility index were determined...
November 2016: American Journal of Reproductive Immunology: AJRI
Florian Kälble, Charlotte Mai, Miriam Wagner, Linda Schober, Matthias Schaier, Martin Zeier, Julia Spratte, Herbert Fluhr, Andrea Steinborn
PROBLEM: Recent studies revealed appropriate differentiation of recent thymic emigrant (RTE)-regulatory T cells (Tregs) to be crucial for maintaining healthy pregnancy. Currently, the role of responder T cells (Tresps) is not known. METHOD OF STUDY: Six-color flow cytometric analysis was used to detect differences in the differentiation of highly proliferative inducible co-stimulatory (ICOS)(+) -RTE-Tregs/Tresps and apoptosis-sensitive ICOS(-) -RTE-Tregs/Tresps into mature naïve (MN)-, CD31(+) -memory and CD31(-) -memory Tregs/Tresps in women with spontaneous preterm labor (sPL) compared to healthy pregnancy...
November 2016: American Journal of Reproductive Immunology: AJRI
Ann E Rozner, Maureen Durning, Jenna Kropp, Gregory J Wiepz, Thaddeus G Golos
PROBLEM: Immune cells within the endometrium at implantation are thought to play an important role in implantation, although their exact role is not well understood. METHOD OF STUDY: A co-culture system of rhesus monkey embryos and maternal immune cells was established. Blastocysts obtained by in vitro fertilization were co-cultured with peripheral blood cells or decidual macrophages. Culture media were collected to assess secretions. Embryo growth was monitored, and trophoblasts were evaluated for proliferation, apoptosis, and differentiation...
November 2016: American Journal of Reproductive Immunology: AJRI
Jiao Fan, Yiping Zhong, Cuina Chen
LABELED PROBLEM: Antinuclear antibody (ANA) could cause reproductive failure. But treatment for women with antinuclear antibody was controversial. METHODS: One hundred and thirty-three ANA+ women with one-time in vitro fertilization (IVF) implantation failure were divided into two groups randomly. The study group comprised 60 sero-positive for antinuclear antibody. These patients were treated with prednisone (10 mg/day) and aspirin (100 mg/day) starting 3 months before induction of ovulation in 60 IVF cycles...
November 2016: American Journal of Reproductive Immunology: AJRI
Carlo Ticconi, Adalgisa Pietropolli, Barbara Borelli, Valentina Bruno, Emilio Piccione, Sergio Bernardini, Nicoletta Di Simone
PROBLEM: To investigate whether antinuclear autoantibodies (ANA) could be related to the outcome of the successive pregnancy in women with unexplained recurrent miscarriage (uRM). METHODS: Circulating ANA were measured by indirect immunofluorescence in 86 women with uRM before pregnancy, repeated in the first trimester of the successive gestation and correlated with pregnancy outcome. RESULTS: Forty-one women were ANA- and 45 were ANA+ before pregnancy...
November 2016: American Journal of Reproductive Immunology: AJRI
Kharah MacKenzie Ross, Gregory Miller, Jennifer Culhane, William Grobman, Hyagriv N Simhan, Pathik D Wadhwa, Douglas Williamson, Thomas McDade, Claudia Buss, Sonja Entringer, Emma Adam, Sameen Qadir, Lauren Keenan-Devlin, Adam K K Leigh, Ann Borders
PROBLEM: Maternal inflammation undergoes adaptations during pregnancy, and excessive inflammation has been associated with adverse outcomes. One mechanism may be maternal inflammation transmission to the fetal compartment. Links between maternal pregnancy inflammation and fetal inflammation are poorly characterized. METHOD: Principal components analysis was used to extract underlying inflammation components across cytokines (IFN-γ, IL-10, IL-13, IL-6, IL-8, TNF-α) in two pregnancy cohorts (SPAH N=87, MOMS N=539) assessed during the second and third trimesters...
November 2016: American Journal of Reproductive Immunology: AJRI
Paulomi Aldo, Yuan You, Klara Szigeti, Tamas L Horvath, Brett Lindenbach, Gil Mor
PROBLEM: Zika virus (ZIKV) has gained public concern for its association with microcephaly in infants born to ZIKV-infected mothers. To reach the fetus the virus must overcome the defense mechanisms provided by trophoblast cells. Additionally, in the first trimester, the integrity of the placenta is critical for fetal protection as damage to differentiating trophoblast can affect placental formation and function. We sought to investigate the effect of ZIKV infection on trophoblast cells and the factors that might increase the risk for ZIKV infection during pregnancy...
November 2016: American Journal of Reproductive Immunology: AJRI
Astrid Marie Kolte, Rudi Steffensen, Ole Bjarne Christiansen, Henriette Svarre Nielsen
PROBLEM: Women with secondary recurrent pregnancy loss (RPL) after a boy have a reduced chance of live birth in the first pregnancy after referral if they carry HY-restricting HLA class II alleles, but long-term chance of live birth is unknown. METHODS OF STUDY: Live birth was compared for 540 women with unexplained secondary RPL according to firstborn's sex and maternal carriage of HLA-DRB3*03:01, HLA-DQB1*05:01/02, HLA-DRB1*15, and HLA-DRB1*07. The groups were compared by Cox proportional hazard ratios...
November 2016: American Journal of Reproductive Immunology: AJRI
Li Qian, Sijiu Yu
PROBLEM: Lycium barbarum (L. barbarum) polysaccharides (LBPs) are the main bioactive component. However, effect of LBP on spermatogenesis of mice with impaired reproduction system induced by cyclophosphamide is undetermined. METHOD OF STUDY: This study aims to investigate the effect of LBP on spermatogenesis of rats with impaired reproduction system induced by cyclophosphamide. Kunming mice were randomly divided into five groups as follows: normal control group, model group (treated with cyclophosphamide) and three LBP groups (treated with cyclophosphamide and 0...
November 2016: American Journal of Reproductive Immunology: AJRI
Ieronymos Voskakis, Christina Tsekoura, Theodora Keramitsoglou, Evangelos Tsantoulas, Efthimios Deligeoroglou, George Creatsas, Marighoula Varla-Leftherioti
PROBLEM: Vγ9Vδ2 T cells (γ9δ2) are involved in antibacterial immune responses. The aim of this study was to look for associations between peripheral blood (PB) γ9δ2 T cells and cervix/vaginal Chlamydia trachomatis (Ct) infection in women with recurrent spontaneous abortions (RSA). METHOD OF STUDY: Peripheral blood samples were obtained from 201 RSA women within 10 days after they experienced a new miscarriage. γ9δ2 T cells and their percentage in total γδ T cells were compared between women who had been found and women who had not been found infected with Ct (last 6 months)...
November 2016: American Journal of Reproductive Immunology: AJRI
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