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Human Cell

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https://www.readbyqxmd.com/read/29330774/a-collection-of-xy-female-cell-lines
#1
Fumio Kasai, Malcolm A Ferguson-Smith
Discordance between sexual phenotype and the 46,XY sex chromosome complement may be found in certain disorders of sexual development (DSD). Many of these DSD patients with female external genitalia and secondary sex characteristics have undescended testes and male internal genitalia. Causative mutations involving genes of the sex determining pathway, including the androgen receptor, SRY and the 5-alpha-reductase genes, are well-known, but the origin of other cases remain unresolved. In this report, we introduce our collection of lymphoblastoid lines derived from female patients with a 46,XY karyotype...
January 12, 2018: Human Cell
https://www.readbyqxmd.com/read/29327117/graphical-classification-of-dna-sequences-of-hla-alleles-by-deep-learning
#2
Jun Miyake, Yuhei Kaneshita, Satoshi Asatani, Seiichi Tagawa, Hirohiko Niioka, Takashi Hirano
Alleles of human leukocyte antigen (HLA)-A DNAs are classified and expressed graphically by using artificial intelligence "Deep Learning (Stacked autoencoder)". Nucleotide sequence data corresponding to the length of 822 bp, collected from the Immuno Polymorphism Database, were compressed to 2-dimensional representation and were plotted. Profiles of the two-dimensional plots indicate that the alleles can be classified as clusters are formed. The two-dimensional plot of HLA-A DNAs gives a clear outlook for characterizing the various alleles...
January 11, 2018: Human Cell
https://www.readbyqxmd.com/read/29313241/cd146-positive-human-dental-pulp-stem-cells-promote-regeneration-of-dentin-pulp-like-structures
#3
Mikiko Matsui, Tomoko Kobayashi, Takeo W Tsutsui
CD146 and STRO-1 are endothelial biomarkers that are co-expressed on the cellular membranes of blood vessels within human dental pulp tissue. This study characterized the percentage of dentin-like structures produced by CD146-positive (CD146+) human dental pulp stem cells (DPSCs), compared with their CD146-negative (CD146-) counterparts. DPSC populations were enriched using magnetic-activated cell sorting (MACS), yielding CD146+ and CD146- cells, as well as mixtures composed of 25% CD146+ cells and 75% CD146- cells (CD146+/-)...
January 8, 2018: Human Cell
https://www.readbyqxmd.com/read/29274035/microrna-30e-regulates-neuroinflammation-in-mptp-model-of-parkinson-s-disease-by-targeting-nlrp3
#4
Dongsheng Li, Hongqi Yang, Jianjun Ma, Sha Luo, Siyuan Chen, Qi Gu
Accumulating evidences suggest that neuroinflammation is a pathological hallmark of Parkinson's disease (PD), a neurodegenerative disorder characterized by loss of dopaminergic neurons in substantia nigra pars compacta (SNpc). MicroRNAs have been recently recognized as crucial regulators of inflammatory responses. Here, we found significant downregulation of microRNA-30e (miR-30e) in SNpc of MPTP-induced PD mice. Next, we employed miR-30e agomir to upregulate miR-30e expression in MPTP-treated mice. Our results showed that delivery of miR-30e agomir remarkably improved motor behavioral deficits and neuronal activity, and inhibited the loss of dopamine neurons...
December 22, 2017: Human Cell
https://www.readbyqxmd.com/read/29249016/therapeutic-activity-of-plant-derived-alkaloid-conophylline-on-metabolic-syndrome-and-neurodegenerative-disease-models
#5
REVIEW
Kazuo Umezawa, Itaru Kojima, Siro Simizu, Yinzhi Lin, Hitomi Fukatsu, Naoki Koide, Yukiomi Nakade, Masashi Yoneda
Increasing metabolic syndromes including type-2 diabetes mellitus, obesity, and steatohepatitis are serious problems in most countries in the world. Neurodegenerative diseases such as Alzheimer, Parkinson's, and Huntington's diseases are increasing in many countries. However, therapy for these diseases is not sufficient yet. Thus, effective chemotherapy for these diseases is being expected. Conophylline is an alkaloid isolated from the leaves of Ervatamia microphylla and related plants. It was found to induce beta-cell differentiation in the precursor pancreatic cells...
December 16, 2017: Human Cell
https://www.readbyqxmd.com/read/29235053/classification-of-c2c12-cells-at-differentiation-by-convolutional-neural-network-of-deep-learning-using-phase-contrast-images
#6
Hirohiko Niioka, Satoshi Asatani, Aina Yoshimura, Hironori Ohigashi, Seiichi Tagawa, Jun Miyake
In the field of regenerative medicine, tremendous numbers of cells are necessary for tissue/organ regeneration. Today automatic cell-culturing system has been developed. The next step is constructing a non-invasive method to monitor the conditions of cells automatically. As an image analysis method, convolutional neural network (CNN), one of the deep learning method, is approaching human recognition level. We constructed and applied the CNN algorithm for automatic cellular differentiation recognition of myogenic C2C12 cell line...
December 13, 2017: Human Cell
https://www.readbyqxmd.com/read/29168129/flavone-protects-hbe-cells-from-dna-double-strand-breaks-caused-by-pm2-5
#7
Xing Ren, Yong Tang, Jiameng Sun, Jianbo Feng, Leilei Chen, Huixi Chen, Sijing Zeng, Changhui Chen, Xinqiu Li, Haixia Zhu, Zhaojun Zeng
Ambient air particulate matter 2.5 (PM2.5) contains many harmful components that can enter the circulatory system and produce reactive oxygen species (ROS) in body. Oxidative stress and DNA damage induced by ROS may affect any cellular macromolecule and lead to DNA double-strand breaks (DSBs). Flavonoids, widely distributed in some herbs and berries, have been proved having anti-oxidative or anti-cancer efficacy. In this study, we investigated whether Flavone, a kind of flavonoids, can protect human bronchial epithelial cells (HBE) from DSBs caused by PM2...
November 22, 2017: Human Cell
https://www.readbyqxmd.com/read/29110251/inhibition-of-two-gastric-cancer-cell-lines-induced-by-fucoxanthin-involves-downregulation-of-mcl-1-and-stat3
#8
Rui-Xue Yu, Rui-Tao Yu, Zhong Liu
Fucoxanthin is a natural carotenoid that had never been previously demonstrated to have anti-tumor effect on human gastric adenocarcinoma SGC-7901 or BGC-823 cells. Here it was found to inhibit proliferation and induce apoptosis through JAK/STAT signal pathway in these cells; the mechanism by which this occurred was investigated. We find that fucoxanthin significantly increased the number of apoptotic cells by propidium iodide (PI) dye staining and flow cytometry. Fucoxanthin (50 or 75 μM) induced SGC-7901 cells cycle arrest at S phase, while BGC-823 cells arrest at G2/M phase...
November 6, 2017: Human Cell
https://www.readbyqxmd.com/read/29103143/dna-hypermethylation-enhanced-telomerase-reverse-transcriptase-expression-in-human-induced-pluripotent-stem-cells
#9
Ken Takasawa, Yoshikazu Arai, Mayu Yamazaki-Inoue, Masashi Toyoda, Hidenori Akutsu, Akihiro Umezawa, Koichiro Nishino
During reprogramming into human induced pluripotent stem cells (iPSCs), several stem cell marker genes are induced, such as OCT-4, NANOG, SALL4, and TERT. OCT-4, NANOG, and SALL4 gene expression can be regulated by DNA methylation. Their promoters become hypomethylated in iPSCs during reprogramming, leading to their induced expression. However, epigenetic regulation of the TERT gene remains unclear. In this study, we focused on epigenetic regulation of the human TERT gene and identified a differentially methylated region (DMR) at a distal region in the TERT promoter between human iPSCs and their parental somatic cells...
November 4, 2017: Human Cell
https://www.readbyqxmd.com/read/29080939/a-common-variant-of-maf-c-maf-transcriptional-factor-gene-in-the-kidney-is-associated-with-gout-susceptibility
#10
Toshihide Higashino, Hirotaka Matsuo, Yukinori Okada, Hiroshi Nakashima, Seiko Shimizu, Masayuki Sakiyama, Shin Tadokoro, Akiyoshi Nakayama, Makoto Kawaguchi, Mako Komatsu, Asahi Hishida, Masahiro Nakatochi, Hiroshi Ooyama, Junko Imaki, Nariyoshi Shinomiya
Gout is a multifactorial disease characterized by acute inflammatory arthritis, and it is caused as a consequence of hyperuricemia. A recent meta-analysis of genome-wide association studies has newly identified the relationship between serum uric acid (SUA) levels and rs889472, a single nucleotide polymorphism of musculoaponeurotic fibrosarcoma oncogene (MAF/c-MAF). However, it remained unclear whether rs889472 is associated with gout susceptibility. In the present study, we investigate the association between c-MAF rs889472 and gout in Japanese male population...
October 28, 2017: Human Cell
https://www.readbyqxmd.com/read/29075999/mir-107-suppresses-cell-proliferation-and-tube-formation-of-ewing-sarcoma-cells-partly-by-targeting-hif-1%C3%AE
#11
Jiajun Chen, Xin Zhou, Qianren Xiao, Tengyu Wang, Gaohai Shao, Yunyun Li, Zhongzu Zhang
MicroRNAs serve a crucial role in the regulation of malignant biological behavior of Ewing's sarcoma (ES). Abnormal expression of miR-107 has been reported in a cohort of cancers, while its exact function in ES remains unclear. Hence, we explored the expression of miR-107 in ES cells and detected its effects on the malignant phenotype of ES cells. Firstly, we perceived the under-expression of miR-107 in human ES cells contrast with the human mesenchymal stem cells. Over-expression of miR-107 restrained cell proliferation and tube formation, arrested cell cycle progression, and facilitated cell apoptosis in SK-ES-1 and RD-ES cell lines...
October 26, 2017: Human Cell
https://www.readbyqxmd.com/read/29063508/age-related-changes-in-cyclic-phosphatidic-acid-induced-hyaluronic-acid-synthesis-in-human-fibroblasts
#12
Katsura Sano, Mari Gotoh, Kyoko Dodo, Noriaki Tajima, Yoshibumi Shimizu, Kimiko Murakami-Murofushi
Hyaluronic acid is a major component of the extracellular matrix, which is important for skin hydration. As aging brings skin dehydration, we aimed to clarify the mRNA expression of hyaluronic acid-related proteins in human skin fibroblasts from donors of various ages (range 0.7-69 years). Previously, we reported that cyclic phosphatidic acid (cPA), a unique phospholipid mediator, stimulated the expression of HAS2 and increased hyaluronic acid synthesis in human skin fibroblasts (donor age: 3 days). In this study, we measured the mRNA expression of hyaluronic acid-related proteins: hyaluronan synthase (HAS) 1-3, hyaluronidase-1, -2, and hyaluronic acid-binding protein (versican)...
October 23, 2017: Human Cell
https://www.readbyqxmd.com/read/29022274/midazolam-prevents-motor-neuronal-death-from-oxidative-stress-attack-mediated-by-jnk-erk-pathway
#13
Guo-Zheng Li, Hong-Lei Tao, Cheng Zhou, Dong-Dong Wang, Cong-Bin Peng
Midazolam is a sedative used by patients with mechanical ventilation. However, the potential clinical value is not fully explored. In this report, we made use of a neuroblastoma-spinal cord hybrid motor neuron-like cell line NSC34, and elucidated the potential role of Midazolam on these cells under the insult of oxidative stress. We found the protective effect of Midazolam on motor neurons against cytotoxicity induced by the combination of oligomycin A and rotenone (O/R) or phenylarsine oxide. The characteristics of apoptosis, such as the ratio of TUNEL+ cells or the expression level of cleaved Caspase-3, was decreased by 22 or 45% in the presence of Midazolam...
October 11, 2017: Human Cell
https://www.readbyqxmd.com/read/28795385/hsa_circ_0045714-regulates-chondrocyte-proliferation-apoptosis-and-extracellular-matrix-synthesis-by-promoting-the-expression-of-mir-193b-target-gene-igf1r
#14
Bao-Feng Li, Ying Zhang, Jin Xiao, Fei Wang, Mei Li, Xiao-Ze Guo, Hui-Bin Xie, Hong Xia, Bei Chen
In recent years, some studies have been made on the effects of circular RNA (circRNA) in osteoarthritis (OA) and so on; however, its mechanisms remain to be further explored. Studies have shown that tumor necrosis factor-alpha can inhibit hsa_circ_0045714 expression in chondrocytes so as to upregulate miR-193b expression. Dual-luciferase reporter assay showed that insulin-like growth factor 1 receptor (IGF1R) is a key target gene of miR-193b. Hsa_circ_0045714 over-expression does not influence miR-193b expression, but can inhibit its transcriptional activity, thereby upregulating IGF1R expression...
October 2017: Human Cell
https://www.readbyqxmd.com/read/28702842/essential-role-of-sh3gl1-in-interleukin-6-il-6-and-vascular-endothelial-growth-factor-vegf-triggered-p130-cas-mediated-proliferation-and-migration-of-osteosarcoma-cells
#15
En-Qi Li, Jin-Li Zhang
We recently demonstrated that interleukin-6 (IL-6)- and vascular endothelial growth factor (VEGF)-induced osteosarcoma (OS) cell proliferation and migration are parallel to significant increased expression of SH3GL1 and the phosphorylation level of P130(cas). The expression level of SH3GL1 was widely upregulated in human OS tissues, and their overexpression was significantly correlated with more aggressive clinicopathological features. Conversely, depletion of SH3GL1 by adenovirus shRNA abrogates P130(cas) phosphorylation and IL-6- and VEGF-induced OS cell proliferation and migration...
October 2017: Human Cell
https://www.readbyqxmd.com/read/28612331/isolation-and-characterization-of-trophoblasts-from-enzymatic-explants-of-human-term-placenta
#16
Tamara D Kolokoltsova, Irina N Saburina, Irina M Zurina, Anastasia A Gorkun, Nastasia V Kosheleva, Vadim S Repin, Rimma A Poltavtseva, Gennady T Sukhikh
In the present study, we describe a new method of isolation and culture of human villous and extravillous trophoblasts from term placenta. The cultivation of trypsinized placental villous tissue explants, followed by the isolation of cells from outgrowth islets allows for obtaining a cytotrophoblast subpopulation that is free from contamination by other cell types. Compared to other methods, our protocol is mild, simple and effective, does not request costly reagents and provides isolation of the mononuclear cytotrophoblast cell populations free from contamination by other types of placental cells...
October 2017: Human Cell
https://www.readbyqxmd.com/read/28573504/microrna-30a-functions-as-tumor-suppressor-and-inhibits-the-proliferation-and-invasion-of-prostate-cancer-cells-by-down-regulation-of-six1
#17
Qinghuan Zhu, Hongzhi Li, Yingjie Li, Lining Jiang
Increasing reports have demonstrated that aberrant expression of microRNAs (miRNAs) is found in multiple human cancers. Many studies have shown that down-regulated level of miR-30a is in a variety of cancers including prostate cancer (PCa). However, the precise mechanisms of miR-30a in PCa have not been well explored. In this study, we investigated the biological functions and molecular mechanism of miR-30a in PCa cell lines, discussing whether it could be a therapeutic biomarker of PCa in the future. We found that miR-30a is down-regulated in PCa tissues and cell lines...
October 2017: Human Cell
https://www.readbyqxmd.com/read/28527127/evaluation-of-heparg-cells-for-the-assessment-of-indirect-drug-induced-hepatotoxicity-using-inh-as-a-model-substance
#18
Anika Mann, Thomas Pelz, Knut Rennert, Alexander Mosig, Michael Decker, Amelie Lupp
HepaRG cells are widely used as an in vitro model to assess drug-induced hepatotoxicity. However, only few studies exist so far regarding their suitability to detect the effects of drugs requiring a preceding activation via the cytochrome P450 (CYP) system. A prototypic substance is the anti-tuberculosis agent INH, which is metabolized into N-acetylhydrazine, which then triggers hepatotoxicity. Therefore, the aim of the present study was to test if this effect can also be detected in HepaRG cells and if it can be counteracted by the known hepatoprotectant silibinin...
October 2017: Human Cell
https://www.readbyqxmd.com/read/28434172/lactobacillus-paracasei-strain-06tca19-suppresses-inflammatory-chemokine-induced-by-helicobacter-pylori-in-human-gastric-epithelial-cells
#19
Shiro Takeda, Keiji Igoshi, Chuluunbat Tsend-Ayush, Tsendesuren Oyunsuren, Ryoichi Sakata, Yasuhiro Koga, Yuo Arima, Masahiko Takeshita
Helicobacter (H.) pylori infection is an important risk factor for gastric cancer that causes gastric inflammation. Inflammatory chemokines such as interleukin (IL)-8 and regulated on activation normal T cell expressed and secreted (RANTES) are elevated in the gastric mucosa by H. pylori. This study aimed to investigate the effects of Lactobacillus paracasei strain 06TCa19, a probiotic strain, on IL-8 and RANTES expression and production induced by H. pylori using human gastric epithelial cell lines. Strain 06TCa19 was shown to suppress H...
October 2017: Human Cell
https://www.readbyqxmd.com/read/28434171/peroxidatic-cysteine-residue-of-peroxiredoxin-2-separated-from-human-red-blood-cells-treated-by-tert-butyl-hydroperoxide-is-hyperoxidized-into-sulfinic-and-sulfonic-acids
#20
Yo-Ichi Ishida, Mariko Aki, Sohta Fujiwara, Masami Nagahama, Yuki Ogasawara
Peroxiredoxin 2 (Prx2) is a redox enzyme that is abundantly expressed in red blood cells (RBCs) and has been the focus of clinical attention for monitoring the oxidative status. We previously developed a method to quantify the reduced and hyperoxidized forms of Prx2 in human RBCs using reverse-phase high-performance liquid chromatography (HPLC). In the present study, we investigated the hyperoxidative status of Prx2 at the molecular level in a post-translational modification analysis using a liquid chromatography-tandem mass spectrometry (LC-MS/MS) system...
October 2017: Human Cell
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