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Human Cell

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https://www.readbyqxmd.com/read/30386989/icariin-ameliorates-angiotensin-ii-induced-cerebrovascular-remodeling-by-inhibiting-nox2-containing-nadph-oxidase-activation
#1
Huanhuan Dong, Shuping Ming, Jie Fang, Yun Li, Ling Liu
Cerebrovascular smooth muscle cells (SMCs) hyperplasia is an important contributor to cerebrovascular remodeling during hypertension. The aim of present study was to investigate the effects of Icariin on cerebrovascular SMCs proliferation and remodeling and the underlying mechanisms. The results revealed that Icariin administration attenuated the enhanced basilar artery constriction in angiotensin II (AngII)-induced hypertension rat model, as well as the inhibition of basilar artery diameter reduction in response to AngII and phenylephrine...
November 1, 2018: Human Cell
https://www.readbyqxmd.com/read/30306504/subcutaneously-administered-adrenomedullin-exerts-a-potent-therapeutic-effect-in-a-murine-model-of-ulcerative-colitis
#2
Yuta Kinoshita, Seiya Arita, Haruka Murazoe, Kazuo Kitamura, Shinya Ashizuka, Kyoko Inagaki-Ohara
Adrenomedullin (AM) exerts a potent anti-inflammatory effect. Intrarectal or consecutive intravenous administrations of AM reduce pathological manifestations in rodent colitis models. However, in clinical applications, a safer administration route that provides stronger alleviation of patient burden is preferred. We investigated whether subcutaneously administered AM is effective against dextran sulfate sodium (DSS)-induced colitis. C57BL/6J mice were administered 1% DSS in drinking water and received AM at 8, 40 or 80 nmol/kg subcutaneously once a day for 7 consecutive days...
October 10, 2018: Human Cell
https://www.readbyqxmd.com/read/30276761/bio-artificial-bone-formation-model-with-a-radial-flow-bioreactor-for-implant-therapy-comparison-between-two-cell-culture-carriers-porous-hydroxyapatite-and-%C3%AE-tricalcium-phosphate-beads
#3
Hideki Nomoto, Haruka Maehashi, Misako Shirai, Mariko Nakamura, Takahiro Masaki, Yoshihiro Mezaki, Jonghyuk Park, Mamoru Aizawa, Kiyoshi Ohkawa, Kiyotsugu Yoshida, Tomokazu Matsuura
Bone grafting is necessary before dental implant treatment in patients with jaw bone defects. Currently, autologous bone grafting is a major burden on the patient. However, it is impossible to form a sufficient foundation for the implant with a bone-filling agent alone. It is, therefore, necessary to prepare hybrid artificial bone tissue containing osteoblasts and osteoclasts. In this study, mouse MC3T3-E1 pre-osteoblast cells and human embryonic-derived osteoblastic cell line hFOB1.19 were cultured in radial-flow bioreactors (RFB) to form three-dimensional artificial bone filled with porous beads of β-tricalcium phosphate (β-TCP) or hydroxyapatite (HA)-which are clinically used as bone-filling agents-as cell culture carriers...
October 1, 2018: Human Cell
https://www.readbyqxmd.com/read/30225781/down-regulation-of-lnctcf7-inhibits-cell-migration-and-invasion-in-colorectal-cancer-via-inhibiting-tcf7-expression
#4
Baihe Wu, Meizhu Chen, Minzhao Gao, Yunyan Cong, Lifeng Jiang, Jinqi Wei, Jin Huang
The prognosis of colorectal cancer (CRC) is still very poor, owing to the high incidence of metastasis. Long noncoding RNA TCF7 (lncTCF7) has been shown to play critical roles in human CRC development and progression, but the molecular mechanisms of lncTCF7 in CRC are still unknown. This study aimed to explore the functions and molecular mechanisms of lncTCF7 on the migration and invasion of CRC cells. Notably, lncTCF7 was highly expressed in CRC cell lines relative to normal colonic epithelial cells. LncTCF7 knockdown significantly inhibited migration and invasion of CRC cells...
September 17, 2018: Human Cell
https://www.readbyqxmd.com/read/30171458/establishment-and-characterization-of-human-gingival-squamous-cell-carcinoma-cell-line-nocs-1
#5
Haruka Takahashi, Miho Watanabe, Akihiro Ohyama, Junko Toyomura, Toshiaki Tachibana, Akira Tanaka, Hiroshi Ishikawa
The NOCS-1 cell line was established from the left gingiva tumor in an 86-year-old Japanese man. Histopathological diagnosis of the original tumor was well-differentiated squamous cell carcinoma. NOCS-1 cells were adhesive epithelial cells with neoplastic or pleomorphic features and grew without contact inhibition. It has been subcultured 70 times during the past 26 months. From passage 3, melanin-containing cells began to be observed in the NOCS-1 cell line. The plating efficiencies were 25% and 23%, doubling times were 29 and 26 h, and saturation densities were 6...
August 31, 2018: Human Cell
https://www.readbyqxmd.com/read/30097922/mir-499-prdm16-axis-modulates-the-adipogenic-differentiation-of-mouse-skeletal-muscle-satellite-cells
#6
Juan Jiang, PengZhou Li, Hao Ling, ZhouZhou Xu, Bo Yi, Shaihong Zhu
Obesity is associated with increased risks of diverse diseases; brown adipose tissue (BAT) can increase energy expenditure and protect against obesity by increasing the decomposition of white adipose tissue (WAT) to enhance the non-coupled oxidative phosphorylation of fatty acid in adipocytes and contributes to weight loss. However, BAT is abundant in only small rodents and newborn humans, but not in adults. PRDM16 is a key factor that induces the differentiation of skeletal muscle precursors to brown adipocytes and simultaneously inhibits myogenic differentiation...
October 2018: Human Cell
https://www.readbyqxmd.com/read/30088225/mir-323a-3p-suppressed-the-glycolysis-of-osteosarcoma-via-targeting-ldha
#7
Hanwen Chen, Shuming Gao, Cai Cheng
Accumulating evidence has demonstrated that there is critical involvement of miRNAs in the initiation and progression of cancers. Here, we showed that miR-323a-3p was significantly down-regulated in osteosarcoma (OS) tissues and cell lines. Overexpression of miR-323a-3p decreased the cell viability, colon formation and induced the apoptosis of OS cells. Using bioinformatics analysis, lactate dehydrogenase A (LDHA) was predicted as one of the down-steam targets of miR-323a-3p. Highly expressed miR-323a-3p significantly decreased both the mRNA and protein levels of LDHA...
October 2018: Human Cell
https://www.readbyqxmd.com/read/29948879/zoledronate-increases-enrichment-activation-and-expansion-of-natural-killer-cells-from-umbilical-cord-blood
#8
LETTER
Zhijie Ma, Yunhong Wang, Huiyan Kang, Xiaoyun Wu
No abstract text is available yet for this article.
October 2018: Human Cell
https://www.readbyqxmd.com/read/29907935/circulating-mir-215-5p-and-mir-642a-5p-as-potential-biomarker-for-diagnosis-of-osteosarcoma-in-mexican-population
#9
Lucero Monterde-Cruz, Eric G Ramírez-Salazar, Genaro Rico-Martínez, Luis M Linares-González, Roberto Guzmán-González, Ernesto Delgado-Cedillo, Eréndira Estrada-Villaseñor, Margarita Valdés-Flores, Rafael Velázquez-Cruz, Alberto Hidalgo-Bravo
Osteosarcoma is the most common malignant bone neoplasia affecting individuals in the second decade of life. The survival rate has not been improved during the last 25 years, in part because of the lack of specific markers. The microRNAs have been identified as important regulators of gene expression, experimental evidence suggests these molecules as key players in cancer development and progression. To identify miRNAs differentially expressed in serum from patients with osteosarcoma compared to healthy donors in Mexican population...
October 2018: Human Cell
https://www.readbyqxmd.com/read/29774518/huh-7-reference-genome-profile-complex-karyotype-composed-of-massive-loss-of-heterozygosity
#10
Fumio Kasai, Noriko Hirayama, Midori Ozawa, Motonobu Satoh, Arihiro Kohara
Human cell lines represent a valuable resource as in vitro experimental models. A hepatoma cell line, HuH-7 (JCRB0403), has been used extensively in various research fields and a number of studies using this line have been published continuously since it was established in 1982. However, an accurate genome profile, which can be served as a reliable reference, has not been available. In this study, we performed M-FISH, SNP microarray and amplicon sequencing to characterize the cell line. Single cell analysis of metaphases revealed a high level of heterogeneity with a mode of 60 chromosomes...
July 2018: Human Cell
https://www.readbyqxmd.com/read/29687375/acetylshikonin-attenuates-angiotensin-ii-induced-proliferation-and-motility-of-human-brain-smooth-muscle-cells-by-inhibiting-wnt-%C3%AE-catenin-signaling
#11
Zequn Li, Zhiyuan Yan, Chunbo Xu, Yiqun Dong, Ye Xiong, Yongyue Dai
Cerebrovascular smooth muscle cell proliferation and migration contribute to hyperplasia in case of cerebrovascular remodeling and stroke. In the present study, we investigated the effects of acetylshikonin, the main ingredient of a Chinese traditional medicine Zicao, on human brain vascular smooth muscle cell (HBVSMCs) proliferation and migration induced by angiotensin II (AngII), and the underlying mechanisms. We found that acetylshikonin treatment significantly inhibited AngII-induced HBVSMCs proliferation and cell cycle transition from G1 to S phase...
July 2018: Human Cell
https://www.readbyqxmd.com/read/29679339/long-non-coding-rna-lncrna-magi2-as3-inhibits-breast-cancer-cell-growth-by-targeting-the-fas-fasl-signalling-pathway
#12
Yong Yang, Hong Yang, Miao Xu, Haibin Zhang, Mingtao Sun, Peng Mu, Tongbao Dong, Shanmei Du, Kui Liu
Long non-coding RNAs (lncRNAs) are non-protein-coding transcripts shown to play important roles in tumourigenesis and tumour progression. Our study aimed to examine expression of the lncRNA MAGI2-AS3 in breast cancer and to explore its function in cancer cell growth. First, MAGI2-AS3 expression levels in clinical samples and cell lines were determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). The functional significance of MAGI2-AS3 in cancer cell proliferation and apoptosis was then examined in vitro...
July 2018: Human Cell
https://www.readbyqxmd.com/read/29671258/atrial-overexpression-of-microrna-27b-attenuates-angiotensin-ii-induced-atrial-fibrosis-and-fibrillation-by-targeting-alk5
#13
Yanshan Wang, Heng Cai, Hongmei Li, Zhisheng Gao, Kunqing Song
Atrial fibrosis influences atrial fibrillation (AF) development by transforming growth factor beta 1 (TGF-β1)/Smad pathway. Although microRNAs are implicated in the pathogenesis of various diseases, information regarding the functional role of microRNAs in atrial dysfunction is limited. In the present study, we found that microRNA-27b (miR-27b) was the dominant member of miR-27 family expressed in left atrium. Moreover, the expression of miR-27b was significantly reduced after angiotensin II (AngII) infusion...
July 2018: Human Cell
https://www.readbyqxmd.com/read/29611131/the-rare-disease-bank-of-japan-establishment-current-status-and-future-challenges
#14
Mayako Tada, Makoto Hirata, Mitsuho Sasaki, Ryuichi Sakate, Arihiro Kohara, Ichiro Takahashi, Yosuke Kameoka, Toru Masui, Akifumi Matsuyama
Research on rare diseases cannot be performed without appropriate samples from patients with such diseases. Due to the limited number of such patients, securing biosamples of sufficient quality for extensive research is a challenge and represents an important barrier to the advancement of research on rare diseases. To tackle this problem, the Rare Disease Bank (RDB) was established in 2009 at the National Institute of Biomedical Innovation (NIBIO; currently, the National Institutes of Biomedical Innovation, Health and Nutrition in Japan)...
July 2018: Human Cell
https://www.readbyqxmd.com/read/29594950/the-effect-of-human-sperm-chromatin-maturity-on-icsi-outcomes
#15
Kamil Gill, Aleksandra Rosiak, Dariusz Gaczarzewicz, Joanna Jakubik, Rafal Kurzawa, Anna Kazienko, Anna Rymaszewska, Maria Laszczynska, Elzbieta Grochans, Malgorzata Piasecka
Because sperm chromatin may play a key role in reproductive success, we verify the associations between sperm chromatin abnormalities, embryo development and the ability to achieve pregnancy. The evaluation of sperm chromatin maturity using aniline blue (AB), chromomycin A3 (CMA3) and toluidine blue (TB) staining were carried out in group of males from infertile couples that underwent ICSI. Low levels of sperm chromatin abnormalities (< 16%) were found in most subjects (> 50%). A higher percentage of TB-positive sperm cells were discovered in the men from couples who achieved ≤ 50% fertilized oocytes compared to men who achieved > 50%...
July 2018: Human Cell
https://www.readbyqxmd.com/read/29582366/the-reversal-effect-of-ginsenoside-rh2-on-drug-resistance-in-human-colorectal-carcinoma-cells-and-its-mechanism
#16
Gui-Wei Liu, Yan-Hua Liu, Guo-Sheng Jiang, Wei-Dan Ren
Recent studies hint that Ginsenoside is involved in cancer prevention and treatment. In this study, we investigated the effect of Ginsenoside Rh2 on drug resistance in human colorectal carcinoma (CRC) cells and its mechanism. The resistance reversion effect of Ginsenoside Rh2 in CRC cells was analyzed using CCK-8 assay. After treating with Ginsenoside Rh2, the cell cycle distribution and cellular apoptosis were analyzed by flow cytometry, cell migration was determined by transwell migration assay, the expression of drug-resistance genes and proteins were evaluated using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot, respectively...
July 2018: Human Cell
https://www.readbyqxmd.com/read/29549584/acetylshikonin-from-zicao-ameliorates-renal-dysfunction-and-fibrosis-in-diabetic-mice-by-inhibiting-tgf-%C3%AE-1-smad-pathway
#17
Zezhao Li, Zhen Hong, Zhiqing Peng, Yongcai Zhao, Rusheng Shao
Diabetic nephropathy (DN) is the major cause of end-stage renal disease in diabetic patients. Zicao, a well-known Chinese traditional medicine, has attracted much attention due to its beneficial effects in various medical fields. In this study, we attempted to investigate the effects and mechanisms of action of acetylshikonin, the main ingredient of Zicao, on renal dysfunction in DN. Our results showed that administration with acetylshikonin not only decreased blood urea nitrogen, urine creatinine and the mean kidney-to-body weight ratio in streptozotocin-induced diabetic mice, but also restored the loss of body weight, whereas the blood glucose was not changed...
July 2018: Human Cell
https://www.readbyqxmd.com/read/29536411/mir-192-5p-suppresses-the-growth-of-bladder-cancer-cells-via-targeting-yin-yang-1
#18
Decai Ji, Lining Jiang, Yingjie Li
Bladder cancer has been identified as one of the most malignant cancers with high incidence and mortality. The underlying mechanisms by which regulate the tumorigenesis of bladder cancer deserve further investigation. Here, we found that miR-192-5p was downregulated in human bladder cancer cell lines and tissues. Overexpression of miR-192-5p significantly inhibited the growth of bladder cancer cells, while depletion of miR-192-5p exerted opposite effect. Bioinformatics analysis and molecular mechanism study identified that miR-192-5p targeted the transcription factor Yin Yang 1 (YY1) and decreased the expression level of YY1...
July 2018: Human Cell
https://www.readbyqxmd.com/read/29876827/the-significance-of-scirrhous-gastric-cancer-cell-lines-the-molecular-characterization-using-cell-lines-and-mouse-models
#19
REVIEW
Masakazu Yashiro, Tasuku Matsuoka, Masaichi Ohira
Scirrhous gastric cancer (SGC) exhibits aggressiveness of the rapid infiltrating tumor cells with abundant fibroblasts. Experimental studies using SGC cell lines have obtained useful information about this cancer. Our literature search divulged a total of 18 SGC cell lines; two cell lines were established from primary SGC and the other lines were established from a metastatic lesion of SGC. Fibroblast growth factor receptor 2 (FGFR2) and transforming growth factor-beta receptor (TβR) are linked to the rapid development of SGC...
June 6, 2018: Human Cell
https://www.readbyqxmd.com/read/29736634/expression-of-mir-132-in-down-syndrome-subjects
#20
LETTER
Michele Salemi, Concetta Barone, Maria Grazia Salluzzo, Mariaconcetta Giambirtone, Federico Ridolfo, Corrado Romano
No abstract text is available yet for this article.
May 8, 2018: Human Cell
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