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Human Cell

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https://www.readbyqxmd.com/read/28326487/enhanced-stim1-expression-is-associated-with-acquired-chemo-resistance-of-cisplatin-in-osteosarcoma-cells
#1
Xilong Sun, Qiang Wei, Jie Cheng, Yanzhu Bian, Congna Tian, Yujing Hu, Huijie Li
Osteosarcoma is the most common primary malignant bone tumor. Although cisplatin is the primary chemotherapy used in osteosarcoma treatment, the cisplatin resistance remains a big challenge for improving overall survival. The store-operated calcium (Ca(2+)) entry (SOCE) and its major mediator Stim1 have been shown to be implicated in a number of pathological processes typical for cancer. In this study, we showed that Stim1 expression was significantly increased in chemo-resistant osteosarcoma tissues compared with chemo-sensitivity tissues...
March 22, 2017: Human Cell
https://www.readbyqxmd.com/read/28260147/establishment-and-characterization-of-a-squamous-cell-carcinoma-cell-line-designated-hzk-1-derived-from-a-metastatic-lymph-node-tumor-of-the-tongue
#2
Tetsuya Hirabayashi, Haruka Takahashi, Miho Watanabe, Toshiaki Tachibana
The hZK-1 cell line was successfully established from the metastatic foci of a lymph node of an 82-year-old Japanese woman with squamous cell carcinoma of the tongue. The pathological diagnosis of the tumor was moderately to well-differentiated squamous cell carcinoma. The hZK-1 cells were angular in shape, and had neoplastic and pleomorphic features. Adjacent hZK-1 cells were joined by desmosomes and well-developed microvilli, and many free ribosomes were observed in the cytoplasm. The doubling time of the hZK-1 cells was approximately 36, 33, and 29 h at the 10th, 20th, and 30th passages, respectively...
March 4, 2017: Human Cell
https://www.readbyqxmd.com/read/28251559/microrna-98-inhibits-tgf-%C3%AE-1-induced-differentiation-and-collagen-production-of-cardiac-fibroblasts-by-targeting-tgfbr1
#3
Ranran Cheng, Ruiying Dang, Yan Zhou, Min Ding, Huikun Hua
To investigate the effects of miR-98 on TGF-β1-induced cardiac fibrosis in human cardiac fibroblasts (HCFs), and to establish the mechanism underlying these effects, HCFs were transfected with miR-98 inhibitor or mimic, and then treated with or without TGF-β1. The level of miR-98 was determined by qRT-PCR in TGF-β1-induced HCFs. Cell differentiation and collagen accumulation of HCFs were detected by qRT-PCR and Western blot assays, respectively. The mRNA and protein expressions of TGFBR1 were determined by qRT-PCR and Western blotting...
March 1, 2017: Human Cell
https://www.readbyqxmd.com/read/28251558/cryopreservation-of-very-low-numbers-of-spermatozoa-from-male-patients-undergoing-infertility-treatment-using-agarose-capsules
#4
Shota Hatakeyama, Susumu Tokuoka, Hiroyuki Abe, Yasuyuki Araki, Yasuhisa Araki
This study tried to cryopreserve low numbers of spermatozoa from men undergoing infertility treatments by inserting into agarose capsules. The capsules were transferred into a drop of cryoprotectant solution and injected 3-4 motile spermatozoa that were selected by the swim-up method by conventional intracytoplasmic sperm injection. These capsules were put on a Cryotop(®) and frozen in liquid nitrogen vapor, and then submerged into liquid nitrogen and subsequently thawed and recovered. The motile spermatozoa in the capsules were counted...
March 1, 2017: Human Cell
https://www.readbyqxmd.com/read/28251557/establishment-and-characterization-of-a-platinum-and-paclitaxel-resistant-high-grade-serous-ovarian-carcinoma-cell-line
#5
Pang-Ning Teng, Nicholas W Bateman, Guisong Wang, Tracy Litzi, Brian E Blanton, Brian L Hood, Kelly A Conrads, Wei Ao, Kate E Oliver, Kathleen M Darcy, William P McGuire, Keren Paz, David Sidransky, Chad A Hamilton, G Larry Maxwell, Thomas P Conrads
High grade serous ovarian cancer (HGSOC) patients have a high recurrence rate after surgery and adjuvant chemotherapy due to inherent or acquired drug resistance. Cell lines derived from HGSOC tumors that are resistant to chemotherapeutic agents represent useful pre-clinical models for drug discovery. Here, we describe establishment of a human ovarian carcinoma cell line, which we term WHIRC01, from a patient-derived mouse xenograft established from a chemorefractory HGSOC patient who did not respond to carboplatin and paclitaxel therapy...
March 1, 2017: Human Cell
https://www.readbyqxmd.com/read/28238196/focal-adhesion-kinase-maintains-but-not-increases-the-adhesion-of-dental-pulp-cells
#6
Yuyan Qian, Meiying Shao, Wenlin Zou, Linyan Wang, Ran Cheng, Tao Hu
Focal adhesion kinase (FAK) functions as a key enzyme in the integrin-mediated adhesion-signalling pathway. Here, we aimed to investigate the effects of FAK on adhesion of human dental pulp (HDP) cells. We transfected lentiviral vectors to silence or overexpress FAK in HDP cells ex vivo. Early cell adhesion, cell survival and focal contacts (FCs)-related proteins (FAK and paxillin) were examined. By using immunofluorescence, the formation of FCs and cytoskeleton was detected, respectively. We found that both adhesion and survival of HDP cells were suppressed by FAK inhibition...
February 25, 2017: Human Cell
https://www.readbyqxmd.com/read/28210976/regenerative-medicine-for-parkinson-s-disease-using-differentiated-nerve-cells-derived-from-human-buccal-fat-pad-stem-cells
#7
Haruka Takahashi, Hiroshi Ishikawa, Akira Tanaka
The purpose of this study was to evaluate the utility of human adipose stem cells derived from the buccal fat pad (hBFP-ASCs) for nerve regeneration. Parkinson's disease (PD) is a neurodegenerative disorder characterized by progressive death of dopaminergic neurons. PD is a candidate disease for cell replacement therapy because it has no fundamental therapeutic methods. We examined the properties of neural-related cells induced from hBFP-ASCs as a cell source for PD treatment. hBFP-ASCs were cultured in neurogenic differentiation medium for about 2 weeks...
February 16, 2017: Human Cell
https://www.readbyqxmd.com/read/28168362/practical-methods-for-handling-human-periodontal-ligament-stem-cells-in-serum-free-and-serum-containing-culture-conditions-under-hypoxia-implications-for-regenerative-medicine
#8
Dai Murabayashi, Mai Mochizuki, Yuichi Tamaki, Taka Nakahara
Stem cell-based therapies depend on the reliable expansion of patient-derived mesenchymal stem cells (MSCs) in vitro. The supplementation of cell culture media with serum is associated with several risks; accordingly, serum-free media are commercially available for cell culture. Furthermore, hypoxia is known to accelerate the expansion of MSCs. The present study aimed to characterize the properties of periodontal ligament-derived MSCs (PDLSCs) cultivated in serum-free and serum-containing media, under hypoxic and normoxic conditions...
February 6, 2017: Human Cell
https://www.readbyqxmd.com/read/28093676/aryl-hydrocarbon-receptor-signaling-involved-in-the-invasiveness-of-lncap-cells
#9
Hisamitsu Ide, Yan Lu, Jingsong Yu, Takahiro Noguchi, Mayuko Kanayama, Satoru Muto, Raizo Yamaguchi, Suguru Kawato, Shigeo Horie
There is now mounting evidence that the aryl hydrocarbon receptor (AhR) plays an important role in physiologic responses such as development, cell cycle regulation, immune function and also malignant transformation in various tissues. The strong nuclear AhR expression is observed in the invasive phenotype, and an elevated nuclear AhR expression is associated with a poor prognosis of human prostate cancer. On the other hand, there are conflicting results that the AhR deficiency results in increased susceptibility to prostate tumors in mouse model...
January 16, 2017: Human Cell
https://www.readbyqxmd.com/read/28091828/liver-x-receptor-%C3%AE-lxr%C3%AE-promoted-invasion-and-emt-of-gastric-cancer-cells-by-regulation-of-nf-%C3%AE%C2%BAb-activity
#10
Linhua Ji, Bin Zhang, Gang Zhao
Aberrant expression of Liver X receptor α (LXRα) has been frequently reported in various types of cancers excluding gastric cancer (GC). Moreover, the role of LXRα in human GC has not been previously reported. In this study, we investigated the effect of LXRα down-regulation on invasion and EMT of GC. The expression of LXRα in GC cell lines was detected by real-time PCR. The LXRα siRNA was transiently transfected into GC cells using Lipofectamine™ 2000 reagent. Subsequently, cell invasive ability was evaluated by Transwell assays...
January 16, 2017: Human Cell
https://www.readbyqxmd.com/read/28070874/infection-of-defective-human-t-lymphotropic-virus-type-1
#11
Yuuki Hashikura, Kazumi Umeki, Kunihiko Umekita, Hajime Nomura, Akiteru Yamada, Ikuo Yamamoto, Hiroo Hasegawa, Katsunori Yanagihara, Akihiko Okayama
In a previous study, we reported that an identical defective provirus had integrated into multiple sites of the genome of a representative human T-lymphotropic virus type 1 (HTLV-1) cell line, MT-2. A possible explanation for this may be the repeated infection of this defective provirus to a cell. Therefore, we attempted to determine whether a defective provirus could transmit during the co-culture of HTLV-1 uninfected human T-cell line, Jurkat, with MT-2 cells treated with mitomycin C. As a result, we established not only a cell line with the integration of one complete provirus, but also a cell line with the integration of one defective provirus...
January 9, 2017: Human Cell
https://www.readbyqxmd.com/read/28058621/mesenchymal-stroma-cells-in-peritoneal-dialysis-effluents-from-patients
#12
Bin Liu, Qiunong Guan, Jing Li, Gerald da Roza, Hao Wang, Caigan Du
Mesenchymal stroma cells (MSCs) have potential as an emerging cell therapy for treating many different diseases, but discovery of the practical sources of MSCs is needed for the large-scale clinical application of this therapy. This study was to identify MSCs in peritoneal dialysis (PD) effluents that were discarded after PD. The effluents were collected from patients who were on the dialysis for less than 1 month. Adherent cells from the effluents were isolated by incubation in serum-containing medium in plastic culture dishes...
January 5, 2017: Human Cell
https://www.readbyqxmd.com/read/27826801/erratum-to-glycogen-synthase-kinase-3-gsk3-inhibitor-sb216763-promotes-the-conversion-of-human-umbilical-cord-mesenchymal-stem-cells-into-neural-precursors-in-adherent-culture
#13
Liyang Gao, Mingyan Zhao, Peng Li, Junchao Kong, Zhijun Liu, Yonghua Chen, Rui Huang, Jiaqi Chu, Juanhua Quan, Rong Zeng
No abstract text is available yet for this article.
January 2017: Human Cell
https://www.readbyqxmd.com/read/27785690/small-cell-lung-cancer-an-epithelial-to-mesenchymal-transition-emt-like-cancer-significance-of-inactive-notch-signaling-and-expression-of-achaete-scute-complex-homologue-1
#14
REVIEW
Takaaki Ito, Shinji Kudoh, Takaya Ichimura, Kosuke Fujino, Wael Ahmed Maher Abdo Hassan, Naoko Udaka
Small cell lung cancer (SCLC) is one of the most malignant neoplasms in common human cancers. The tumor is composed of small immature-looking cells with a round or fusiform shape, which possesses weak adhesion features among them, suggesting that SCLC shows the morphological characteristics of epithelial to mesenchymal transition (EMT). SCLC is characterized by high metastatic and recurrent rates, sensitivity to the initial chemotherapy, and easy acquirement of chemoresistance afterwards. These characters may be related to the EMT phenotype of SCLC...
January 2017: Human Cell
https://www.readbyqxmd.com/read/27766570/long-noncoding-rna-tcf7-promotes-invasiveness-and-self-renewal-of-human-non-small-cell-lung-cancer-cells
#15
Jinhui Wu, Dongshuang Wang
Lung cancer is the most common solid tumor and the leading cause of cancer-related death worldwide. Non-small cell lung cancer (NSCLC) represents the major histological subtype and accounts for about 80 % cases of lung cancer cases. Recently, lncRNA lncTCF7 was identified, which is highly expressed in hepatocellular carcinoma (HCC) tumors and liver cancer stem cells (CSCs). However, the role of lnTCF7 in NSCLC remains largely unknown. In this study, Gain- and loss-of-function studies demonstrated the critical role of lncTCF7 in promoting invasion and self-renewal in NSCLC cells...
January 2017: Human Cell
https://www.readbyqxmd.com/read/27743351/microrna-124-upregulation-inhibits-proliferation-and-invasion-of-osteosarcoma-cells-by-targeting-sphingosine-kinase-1
#16
Yan Zhou, Yanzhen Han, Zhitao Zhang, Zhe Shi, Liyuan Zhou, Xiaohong Liu, Xiaoyan Jia
Increasing evidence has confirmed that the dysregulation of microRNAs (miRNAs) contributes to the proliferation and invasion of human cancers. Previous studies have shown that the dysregulation of miR-124 is in numerous cancers. However, the roles of miR-124 in human osteosarcoma (OS) have not been well clarified. Therefore, this study was to investigate the biological functions and molecular mechanisms of miR-124 in OS cell lines, discussing whether it could be a therapeutic biomarker of OS in the future. In this study, our results demonstrated that miR-124 was down-regulated in OS cell lines and tissues...
January 2017: Human Cell
https://www.readbyqxmd.com/read/27613543/molecular-cytogenetic-characterization-of-two-established-esft-cell-lines
#17
Masako Ishiguro, Mutsumi Yuki, Tomoko Fukushige, Mikio Mizoguchi, Yasuhiko Kaneko, Takeshita Morishige, Hiroshi Iwasaki
Ewing's sarcoma/primitive neuroectodermal tumor/Askin's tumor (Ewing`s sarcoma family of tumors: ESFT) is the most common type of malignant tumor of bone and soft tissue in children and young adults, and morphologically is a member of a group of small round cell tumors. We report, here, on the establishment of two human ESFT cell lines, FU-PNET-3 and FU-PNET-4, from the iliac and the chest wall, respectively, the cells of both cell lines were tumorigenic in immunodeficient mice. Histologically, both original and xenograft tumors and cultured cells were composed of small round cells with positive immunoreactivity for CD99 and Nkx2...
January 2017: Human Cell
https://www.readbyqxmd.com/read/27604750/glycogen-synthase-kinase-3-gsk3-inhibitor-sb216763-promotes-the-conversion-of-human-umbilical-cord-mesenchymal-stem-cells-into-neural-precursors-in-adherent-culture
#18
Liyang Gao, Mingyan Zhao, Peng Li, Junchao Kong, Zhijun Liu, Yonghua Chen, Rui Huang, Jiaqi Chu, Juanhua Quan, Rong Zeng
The ability to generate neural progenitor cells from human umbilical cord mesenchymal stem cells (hUC-MSCs) has provided an option to treat neurodegenerative diseases. To establish a method for this purpose, we characterized the early neural markers of hUC-MSCs-derived cells under different conditions. We found that neither the elimination of signals for alternative fate nor N2 supplement was sufficient to differentiate hUC-MSCs into neural precursor cells, but the GSK3 inhibitor SB216763 could promote an efficient neural commitment of hUC-MSCs...
January 2017: Human Cell
https://www.readbyqxmd.com/read/27889902/establishment-and-characterization-of-a-novel-uterine-carcinosarcoma-cell-line-tu-ecs-1-with-mutations-of-tp53-and-kras
#19
Yohei Chiba, Seiya Sato, Hiroaki Itamochi, Yasuko Suga, Tomoyuki Fukagawa, Nao Oumi, Tetsuro Oishi, Tasuku Harada, Tamotsu Sugai, Toru Sugiyama
A new human uterine carcinosarcoma (UCS) cell line, TU-ECS-1, was established and characterized. The morphological appearance of the cultured cells was an insular of epithelial-like cells arranged in the form of a jigsaw puzzle and mesenchymal-like cells with a spindle-shaped or fibroblast-like morphology. A relatively high proliferation rate was observed with a doubling time of 18.2 h. The chromosome number ranged from 44 to 49 and had an extra chromosome 12 (trisomy 12). The respective half-maximal inhibitory concentrations of cisplatin, paclitaxel, and doxorubicin were 2...
November 26, 2016: Human Cell
https://www.readbyqxmd.com/read/27885588/a-mouse-model-reveals-that-mfsd2a-is-critical-for-unfolded-protein-response-upon-exposure-to-tunicamycin
#20
Hiroshi Moritake, Megumi Obara, Yusuke Saito, Ayako Kashimada, Masatoshi Takagi, Megumi Funakoshi-Tago, Tomofusa Fukuyama, Mikio Yoshioka, Akira Inoue, Hiroyuki Komatsu, Hideki Nishitoh, Hiroaki Kataoka, Hiroyuki Nunoi
Major facilitator superfamily domain containing 2a (Mfsd2a) is a member of the major facilitator superfamily. Mfsd2a functions as a transporter for docosahexaenoic acid and also plays a role in the unfolded protein response (UPR) upon tunicamycin (TM) exposure. UPR is involved in the pathogenesis of various human diseases. TM and thapsigargin are representative experimental reagents that induce UPR. To elucidate the detailed function of Mfsd2a in UPR in vivo, we generated Mfsd2a-deficient mice and investigated the role of Mfsd2a during UPR induced by TM or thapsigargin...
November 24, 2016: Human Cell
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