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Zeitschrift Für Naturforschung. C, A Journal of Biosciences

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https://www.readbyqxmd.com/read/28432851/fungal-production-of-the-polysaccharide-pullulan-from-a-plant-hydrolysate
#1
Thomas P West
The ability of the fungus Aureobasidium pullulans ATCC 42023 to produce pullulan from hydrolysates of the native grass known as prairie cordgrass was investigated and examined relative to polysaccharide and cell biomass production, yield, and pullulan content of the polysaccharide. A pullulan concentration of 9.7 g l-1 and yield of 0.78 g g-1 was produced by ATCC 42023 when grown for 168 h at 30°C on a phosphate-buffered hydrolysate. The highest biomass level of 7.7 g l-1 was produced by ATCC 42023 after 168 h on a hydrolysate-containing complete medium...
April 22, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28432850/a-detailed-experimental-study-of-a-dna-computer-with-two-endonucleases
#2
Sebastian Sakowski, Tadeusz Krasiński, Joanna Sarnik, Janusz Blasiak, Jacek Waldmajer, Tomasz Poplawski
Great advances in biotechnology have allowed the construction of a computer from DNA. One of the proposed solutions is a biomolecular finite automaton, a simple two-state DNA computer without memory, which was presented by Ehud Shapiro's group at the Weizmann Institute of Science. The main problem with this computer, in which biomolecules carry out logical operations, is its complexity - increasing the number of states of biomolecular automata. In this study, we constructed (in laboratory conditions) a six-state DNA computer that uses two endonucleases (e...
April 22, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28315853/effects-of-extracts-and-compounds-from-tricholoma-populinum-lange-on-degranulation-and-il-2-il-8-secretion-of-immune-cells
#3
Simon Merdivan, Kristina Jenett-Siems, Karsten Siems, Timo H J Niedermeyer, Nadin Schultze, Carmen Steinborn, Carsten Gründemann, Ulrike Lindequist
Tricholoma populinum Lange is an edible basidiomycete from the family Tricholomataceae. Extracts, fractions, and different metabolites isolated from the fruiting bodies of this mushroom were tested for degranulation-inhibiting activities on RBL-2H3 cells (rat basophils). Dichloromethane extracts decreased degranulation significantly, as did a fraction after column chromatography. In addition, the extract decreased the IL-2 release from Jurkat T cells and the release of IL-8 from HMC-1 human mast cells. The results show the significant effects of extracts of T...
March 20, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28301324/response-of-alternative-splice-isoforms-of-osrad9-gene-from-oryza-sativa-to-environmental-stress
#4
Rui Li, Wenguo Wang, Fosheng Li, Qingwei Wang, Shenghua Wang, Ying Xu, Fang Chen
Rad9 protein plays an important role in cell-cycle checkpoint signal transduction in human and yeast cells, but knowledge about Rad9 in plants is limited. This study reports that the Rad9 gene of rice can generate the transcript products OsRad9.1 and OsRad9.2 through alternative splicing. OsRad9.1, with all nine exons, is the main cell-cycle checkpoint protein involved in the response of rice to genotoxic stresses (ultraviolet radiation and antibiotic stress), environmental stresses (drought, salt, and heavy metal stress), and auxin stimuli (2,4-D, IAA, and IBA)...
March 16, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28306545/scent-gland-constituents-of-the-middle-american-burrowing-python-loxocemus-bicolor-serpentes-loxocemidae
#5
Thies Schulze, Paul J Weldon, Stefan Schulz
Analysis by gas chromatography/mass spectrometry of the scent gland secretions of male and female Middle American burrowing pythons (Loxocemus bicolor) revealed the presence of over 300 components including cholesterol, fatty acids, glyceryl monoalkyl ethers, and alcohols. The fatty acids, over 100 of which were identified, constitute most of the compounds in the secretions and show the greatest structural diversity. They include saturated and unsaturated, unbranched and mono-, di-, and trimethyl-branched compounds ranging in carbon-chain length from 13 to 24...
March 15, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28284041/the-atmyb12-activation-domain-maps-to-a-short-c-terminal-region-of-the-transcription-factor
#6
Ralf Stracke, Neslihan Turgut-Kara, Bernd Weisshaar
The Arabidopsis thaliana R2R3-MYB transcription factor MYB12 is a light-inducible, flavonol-specific activator of flavonoid biosynthesis. The transactivation activity of the AtMYB12 protein was analyzed using a C-terminal deletion series in a transient A. thaliana protoplast assay with the goal of mapping the activation domain (AD). Although the deletion of the last 46 C-terminal amino acids did not affect the activation capacity, the deletion of the last 98 amino acids almost totally abolished transactivation of two different target promoters...
March 11, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28258977/different-sensitivities-of-photosystem-ii-in-green-algae-and-cyanobacteria-to-phenylurea-and-phenol-type-herbicides-effect-on-electron-donor-side
#7
Ekaterina K Yotsova, Martin A Stefanov, Anelia G Dobrikova, Emilia L Apostolova
The effects of short-term treatment with phenylurea (DCMU, isoproturon) and phenol-type (ioxynil) herbicides on the green alga Chlorella kessleri and the cyanobacterium Synechocystis salina with different organizations of photosystem II (PSII) were investigated using pulse amplitude modulated (PAM) chlorophyll fluorescence and photosynthetic oxygen evolution measured by polarographic oxygen electrodes (Clark-type and Joliot-type). The photosynthetic oxygen evolution showed stronger inhibition than the PSII photochemistry...
March 4, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28170343/bioenergetic-strategy-of-microalgae-for-the-biodegradation-of-tyrosol-and-hydroxytyrosol
#8
Aikaterini Papazi, Andreas Ioannou, Myrto Symeonidi, Andreas G Doulis, Kiriakos Kotzabasis
Olive mill wastewater has significant polluting properties due to its high phenolic content [mainly tyrosol (trs) and hydroxytyrosol (htrs)]. Growth kinetics and a series of fluorescence induction measurements for Scenedesmus obliquus cultures showed that microalgae can be tolerant of these phenolic compounds. Changes in the cellular energy reserves and concentration of the phenolic compounds adjust the "toxicity" of these compounds to the microalgae and are, therefore, the main parameters that affect biodegradation...
February 7, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28160544/effects-of-hypericum-perforatum-extract-on-oxaliplatin-induced-neurotoxicity-in-vitro-evaluations
#9
Lorenzo Cinci, Lorenzo Di Cesare Mannelli, Anna Maidecchi, Luisa Mattoli, Carla Ghelardini
Hypericum perforatum L. has been used for centuries as a natural remedy for the treatment of many disorders. Neuropathic pain is a common side effect of oxaliplatin-based chemotherapy and often the cause of therapy discontinuation. Thanks to its anti-inflammatory and analgesic effects, the use of H. perforatum may be a novel therapeutic strategy for neuropathy. The aim of this paper was to evaluate the effect of H. perforatum hydrophilic extract on an in vitro model of oxaliplatin-induced neurotoxicity. The antioxidant potential of extract was first evaluated in cell-free models by the thiobarbituric acid-reactive substances assay and nitro blue tetrazolium oxidation test; the ability of H...
February 4, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28182579/synthesis-and-biological-evaluation-of-newer-1-3-4-oxadiazoles-incorporated-with-benzothiazepine-and-benzodiazepine-moieties
#10
Patel Navin, Patel Sarvil, Purohit Amit, Patel Divyesh, Rajani Dhansukh, Rosa Moo-Puc, Gildardo Rivera
A series of thiazepines and diazepines having 1,3,4-oxadiazole moiety were synthesized, and they were analyzed for their in vitro antimicrobial activity against several bacteria (Staphylococcus aureus, Staphylococcus pyogenes, Escherichia coli, and Pseudomonas aeruginosa) and fungi (Candida albicans, Aspergillus niger, and Aspergillus Clavatus) and protozoa (Entamoeba histolytica, Giardia lamblia, Trypanosoma cruzi and Leishmania mexicana). Few of the selected compounds were tested for their antitubercular activity...
March 1, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28121619/bioenergetics-of-lactate-vs-acetate-outside-tca-enhanced-the-hydrogen-evolution-levels-in-two-newly-isolated-strains-of-the-photosynthetic-bacterium-rhodopseudomonas
#11
Amal W Danial, Ahmed M Abdel Wahab, Houssam H Arafat, Refat Abdel-Basset
Two local hydrogen-evolving strains of purple nonsulfur bacteria have been isolated, characterized, and identified as Rhodopseudomonas sp. TUT (strains Rh1 and Rh2). Lactate followed by succinate and malate supported the highest amounts of H2 production, growth (O.D.660nm, proteins and bacteriochlorphyll contents), nitrogenase activity, and uptake hydrogenase; the least of which was acetate. Alginate-immobilized cells evolved higher hydrogen amounts than free cell counterparts. Rh1 was more productive than Rh2 at all circumstances...
March 1, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28107177/antinociceptive-effect-of-aristolochia-trilobata-stem-essential-oil-and-6-methyl-5-hepten-2yl-acetate-its-main-compound-in-rodents
#12
Jullyana de Souza Siqueira Quintans, Rafael Dos Santos Alves, Darlisson de Alexandria Santos, Mairim Russo Serafini, Péricles Barreto Alves, Emmanoel Vilaça Costa, Gokhan Zengin, Lucindo José Quintans-Júnior, Adriana Gibara Guimarães
Aristolochia trilobata L. is an aromatic plant, popularly known as "mil-homens", and its essential oil (EO) is generally used to treat colic, diarrhea and dysentery disorders. We evaluated the antinociceptive effect of A. trilobata stem EO and of its major compound, the (R)-(-)-6-methyl-5-hepten-2-yl acetate (sulcatyl acetate: SA), using acetic acid (0.85%)-induced writhing response and formalin-induced (20 μL of 1%) nociceptive behavior in mice. We also evaluated the EO and SA effect on motor coordination, using the rota-rod apparatus...
March 1, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/27845890/virus-inactivation-under-the-photodynamic-effect-of-phthalocyanine-zinc-ii-complexes
#13
Mimi Remichkova, Luchia Mukova, Lubomira Nikolaeva-Glomb, Nadya Nikolova, Lubka Doumanova, Vanya Mantareva, Ivan Angelov, Veselin Kussovski, Angel S Galabov
Various metal phthalocyanines have been studied for their capacity for photodynamic effects on viruses. Two newly synthesized water-soluble phthalocyanine Zn(II) complexes with different charges, cationic methylpyridyloxy-substituted Zn(II)- phthalocyanine (ZnPcMe) and anionic sulfophenoxy-substituted Zn(II)-phthalocyanine (ZnPcS), were used for photoinactivation of two DNA-containing enveloped viruses (herpes simplex virus type 1 and vaccinia virus), two RNA-containing enveloped viruses (bovine viral diarrhea virus and Newcastle disease virus) and two nude viruses (the enterovirus Coxsackie B1, a RNA-containing virus, and human adenovirus 5, a DNA virus)...
March 1, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/27740933/environmental-alterations-in-biofuel-generating-molecules-in-zilla-spinosa
#14
Hemmat Khattab, Zeinab El Marid
Now days, production of fuels and petrochemicals from renewable lignocellulosic biomass is an indispensable issue to meet the growing energy demand. Meanwhile, the changes in the climate and soil topography influence the growth and development as well as canopy level of the lignocellulosic biomass. In this study, Zilla spinosa Turr (Zilla) plants with similar age and size were collected from three main sectors (upstream, midstream, and downstream) of Wadi Hagul during spring (April) and summer (July) seasons...
March 1, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/27701142/caspase-1-from-the-silkworm-bombyx-mori-is-involved-in-bombyx-mori-nucleopolyhedrovirus-infection
#15
Qiang Wang, Xiaoli Ju, Liang Chen, Keping Chen
Caspase-1 is one of the effector caspases in mammals that plays a central role in apoptosis. However, the lepidopteran caspase-1, especially the Bombyx mori caspase-1 (Bm-caspase-1), has not been investigated in detail. In this study, Bm-caspase-1 was identified from an expressed sequence tag database in B. mori by BLAST search. The open reading frame of Bm-caspase-1 contained 879 nucleotides and encoded 293 amino acids with a predicted molecular mass of 33 kDa. Bm-caspase-1 contained two consensus amino acid motifs of caspase cleavage sites, DEGDA and TETDG...
March 1, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/27701141/cytochalasin-p1-a-new-cytochalasin-from-the-marine-derived-fungus-xylaria-sp-sof11
#16
Ziming Chen, Yuchan Chen, Hongbo Huang, Hongyan Yang, Weimin Zhang, Yulin Sun, Jing Wen
A new cytochalasin, named cytochalasin P1 (1), together with four known analogs (2-5) was isolated from marine-derived fungus Xylaria sp. SOF11 from the South China Sea. The structure of the new compound was elucidated on the basis of MS and NMR (1H, 13C, HSQC, HMBC, and NOESY) data analyses. Compounds 1-5 were tested for their cytotoxicities against four tumor cell lines (SF-268, MCF-7, NCI-H460, and HepG-2). Compounds 1-5 showed significant cytotoxicity against two tumor cell lines MCF-7 and SF-268, with the IC50 values varying between 0...
March 1, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/27701140/leaves-of-cordia-boissieri-a-dc-as-a-potential-source-of-bioactive-secondary-metabolites-for-protection-against-metabolic-syndrome-induced-in-rats
#17
Asmaa I Owis, Amira M Abo-Youssef, Ahmed H Osman
Cordia boissieri A. DC. (Boraginaceae) is traditionally used as an herbal remedy for diabetes by Hispanic women in Southwestern USA. A recent investigation showed the significant protective effect of ethyl acetate extract against metabolic syndrome (MS). However, the corresponding active principles responsible for this effect and relations between their structure and biological actions remain unclear. Thus, ethyl acetate extract was subjected to column chromatography, which yielded seven compounds identified on the basis of spectroscopic data as rutin, hesperidin, kaempferol-3-O-β-d-glucopyranoside, rosmarinic acid, β-sitosterol-3-O-β-d-glucopyranoside, quercetin, and kaempferol...
March 1, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/27442367/the-evaluation-of-the-synergistic-effect-of-3-2-4-dihydroxyphenyl-propionic-acid-and-l-ascorbic-acid-on-tyrosinase-inhibition
#18
Xinyang Chen, Aya Haniu, Takehiro Kashiwagi, Hiroyuki Watanabe, Takashi Watanabe, Yoshino Okamoto, Masanobu Suzuki, Chul-Sa Kim
3-(2,4-Dihydroxyphenyl)propionic acid (DDPA) and l-ascorbic acid (vitamin C) show tyrosinase inhibition activity. A synergistic effect on tyrosinase inhibition was observed when the two compounds were mixed. The effect significantly decreased the IC50 value of both compounds.
March 1, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28107178/influence-of-structure-of-lactones-with-the-methylcyclohexene-and-dimethylcyclohexene-ring-on-their-biotransformation-and-antimicrobial-activity
#19
Katarzyna Wińska, Małgorzata Grabarczyk, Wanda Mączka, Barbara Żarowska, Gabriela Maciejewska, Mirosław Anioł
The aim of this article is influence of the structure of lactones with the methylcyclohexene and dimethylcyclohexene ring on their biotransformation and antimicrobial activity. This work was based on the general remark that even the smallest change in the structure of a compound can affect its biological properties. The results of the biotransformation of four bicyclic unsaturated lactones with one or two methyl groups in the cyclohexene ring was tested using fifteen fungal strains (Fusarium species, Penicillium species, Absidia species, Cunninghamella japonica, and Pleurotus ostreatus) and five yeast strains (Yarrowia lipolytica, Rhodorula marina, Rhodorula rubra, Candida viswanathii, and Saccharomyces cerevisiae)...
January 20, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28063265/pegylation-potentiates-hepatoma-cell-targeted-liposome-mediated-in-vitro-gene-delivery-via-the-asialoglycoprotein-receptor
#20
Nkosiyethu K Mkhwanazi, Charles B de Koning, Willem A L van Otterlo, Mario Ariatti, Moganavelli Singh
Hepatocellular carcinoma is a burgeoning health issue in sub-Saharan Africa and East Asia where it is most prevalent. The search for gene medicine treatment modalities for this condition represents a novel departure from current treatment options and is gaining momentum. Here we report on nonPEGylated and on sterically stabilized PEGylated cationic liposomes decorated with D-galacto moieties linked to 24.1 Å spacers for asialoglycoprotein receptor (ASGP-R)-targeted vehiculation of pCMV-luc plasmid DNA. Cargo DNA is fully liposome associated at N/P ratio=3:1 and is partially protected from the effects of serum nucleases...
January 7, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
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