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Zeitschrift Für Naturforschung. C, A Journal of Biosciences

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https://www.readbyqxmd.com/read/28672764/interaction-of-tubulin-and-protein-kinase-ck2-in-trypanosoma-equiperdum
#1
Beatriz E Boscán, Graciela L Uzcanga, Maritza Calabokis, Rocío Camargo, Frank Aponte, José Bubis
A polypeptide band with an apparent molecular weight of 55,000 was phosphorylated in vitro in whole-cell lysates of Trypanosoma equiperdum. This band corresponds to tubulin as demonstrated by immunoprecipitation of the phosphorylated polypeptide from T. equiperdum extracts when anti-α and anti-β tubulin monoclonal antibodies were employed. A parasite protein kinase CK2 was in charge of modifying tubulin given that common mammalian CK2 inhibitors such as emodin and GTP, hindered the phosphorylation of tubulin and exogenously added casein...
June 27, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28640755/chemical-composition-and-biological-activities-of-leaf-and-fruit-essential-oils-from-eucalyptus-camaldulensis
#2
Gulden Dogan, Nazan Kara, Eyup Bagci, Seher Gur
The chemical composition of the essential oils from the leaves and fruit of Eucalyptus camaldulensis grown in Mersin, Turkey was analyzed using gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) techniques. The biological activities (antibacterial and antifungal) were examined using the agar well diffusion method. The main leaf oil constituents were p-cymene (42.1%), eucalyptol (1,8-cineole) (14.1%), α-pinene (12.7%) and α-terpinol (10.7%). The main constituents of the fruit oil were eucalyptol (1,8-cineole) (34...
June 22, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28525357/a-dihydrochalcone-derivative-and-further-steroidal-saponins-from-sansevieria-trifasciata-prain
#3
Billy T Tchegnitegni, Rémy B Teponno, Kristina Jenett-Siems, Matthias F Melzig, Tomofumi Miyamoto, Léon A Tapondjou
Phytochemical investigation of the aerial parts of Sansevieria trifasciata, one of the most common Dracaenaceae plants, has resulted in the isolation of a new dihydrochalcone derivative named trifasciatine C (1), four previously unreported steroidal saponins as two pairs of inseparable regioisomers: trifasciatosides K/L (2/3), M/N (4/5), together with the known 1,2-(dipalmitoyl)-3-O-β-D-galactopyranosylglycerol (6), aconitic acid (7), and 1-methyl aconitic acid (8). Their structures were elucidated mainly by extensive spectroscopic analysis (1D and 2D nuclear magnetic resonance) and high-resolution electronspray ionization-mass spectrometry, as well as chemical methods and comparison of their spectral data with those of related compounds...
May 18, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28525356/synthesis-biological-activity-and-molecular-modeling-study-of-new-schiff-bases-incorporated-with-indole-moiety
#4
Ahmed H Halawa, Shimaa Mohamed Abd El-Gilil, Ahmed H Bedair, Mohamed Shaaban, Marcel Frese, Norbert Sewald, Essam M Eliwa, Ahmed M El-Agrody
A new series of heterocyclic Schiff bases 2-9 containing indole moiety were synthesized by facile and efficient condensation of indole-3/2/5-carboxaldehyde (1a/1b/1c) with different aromatic and heterocyclic primary amines using conventional and/or microwave irradiation methods. The structures of the obtained compounds were assigned by sophisticated spectroscopic and spectrometric techniques (1D-NMR, 2D-NMR and MS). The synthesized compounds were screened for their cytotoxicity and antibacterial activities...
May 18, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28525347/sustainable-farming-of-the-mealworm-tenebrio-molitor-for-the-production-of-food-and-feed
#5
Thorben Grau, Andreas Vilcinskas, Gerrit Joop
The farming of edible insects is an alternative strategy for the production of protein-rich food and feed with a low ecological footprint. The industrial production of insect-derived protein is more cost-effective and energy-efficient than livestock farming or aquaculture. The mealworm Tenebrio molitor is economically among the most important species used for the large-scale conversion of plant biomass into protein. Here, we review the mass rearing of this species and its conversion into food and feed, focusing on challenges such as the contamination of food/feed products with bacteria from the insect gut and the risk of rapidly spreading pathogens and parasites...
May 17, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28593879/deciphering-the-route-to-cyclic-monoterpenes-in-chrysomelina-leaf-beetles-source-of-new-biocatalysts-for-industrial-application
#6
Antje Burse, Wilhelm Boland
The drastic growth of the population on our planet requires the efficient and sustainable use of our natural resources. Enzymes are indispensable tools for a wide range of industries producing food, pharmaceuticals, pesticides, or biofuels. Because insects constitute one of the most species-rich classes of organisms colonizing almost every ecological niche on earth, they have developed extraordinary metabolic abilities to survive in various and sometimes extreme habitats. Despite this metabolic diversity, insect enzymes have only recently generated interest in industrial applications because only a few metabolic pathways have been sufficiently characterized...
May 8, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28455953/biotechnology-for-bioenergy-dedicated-trees-meeting-future-energy-demands
#7
Hani Al-Ahmad
With the increase in human demands for energy, purpose-grown woody crops could be part of the global renewable energy solution, especially in geographical regions where plantation forestry is feasible and economically important. In addition, efficient utilization of woody feedstocks would engage in mitigating greenhouse gas emissions, decreasing the challenge of food and energy security, and resolving the conflict between land use for food or biofuel production. This review compiles existing knowledge on biotechnological and genomics-aided improvements of biomass performance of purpose-grown poplar, willow, eucalyptus and pine species, and their relative hybrids, for efficient and sustainable bioenergy applications...
April 29, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28710885/lothar-jaenicke-and-c1-metabolism-his-first-25-years-of-research
#8
Rudolf K Thauer
The biochemist Lothar Jaenicke died on 29 December 2015, aged 92 years old. The last time I saw him was at his 90th birthday on 14 September 2013, at the occasion of which his colleagues at the Universität zu Köln (Cologne) in Germany had organized a symposium to honor him.
July 26, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28710884/lothar-jaenicke-1923-2015-zum-ged%C3%A3-chtnis
#9
Hans E Hummel, Simone S Langner
Among the post-war European biochemists, L. Jaenicke (1923-2015) stands out as a distinctive character with bright ideas and professional elegance, yet with personal modesty. His contributions to diverse fields such as C1 metabolic pathways, signal compounds (aquatic sex attractants) of brown algae and chemotaxis appear as distinctive features not covered by his peers. His rich scientific œuvre is documented in 291 journal articles, nine books, and a huge number of almost 570 book reviews, recensions, biographies and commentaries on the politics of science and the history of biochemistry...
July 26, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28665793/a-succinct-access-to-%C3%AF-hydroxylated-jasmonates-via-olefin-metathesis
#10
Guillermo H Jimenez-Aleman, Selina Seçinti, Wilhelm Boland
In higher plants, jasmonates are lipid-derived signaling molecules that control many physiological processes, including responses to abiotic stress, defenses against insects and pathogens, and development. Among jasmonates, ω-oxidized compounds form an important subfamily. The biological roles of these ω-modified derivatives are not fully understood, largely due to their limited availability. Herein, a brief (two-step), simple and efficient (>80% yield), versatile, gram-scalable, and environmentally friendly synthetic route to ω-oxidized jasmonates is described...
July 14, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28453442/3-7-isoquinoline-quinones-from-the-ascidian-tunicate-ascidia-virginea
#11
Sven T Possner, Frank C Schroeder, Hans Tore Rapp, Volker Sinnwell, Stefan Franke, Wittko Francke
A new isoquinoline quinone system and its iodinated derivatives were isolated from the ascidian tunicate Ascidia virginea Müller 1776 (Phlebobranchia: Ascidiidae). Structures were elucidated by spectroscopic methods and derivatization reactions. Ascidine A (3,7-dihydro-1,8-dihydroxy-4-(4'-hydroxyphenyl)isoquinoline-3,7-dione (1), ascidine B (3,7-dihydro-1,8-dihydroxy-4-(4'-hydroxy-3'-iodophenyl)isoquinoline-3,7-dione (2), and ascidine C (3,7-dihydro-1,8-dihydroxy-4-(4'-hydroxy-3',5'-diiodophenyl)isoquinoline-3,7-dione (3) represent a novel type of tyrosine-derived alkaloids...
July 14, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28432850/a-detailed-experimental-study-of-a-dna-computer-with-two-endonucleases
#12
Sebastian Sakowski, Tadeusz Krasiński, Joanna Sarnik, Janusz Blasiak, Jacek Waldmajer, Tomasz Poplawski
Great advances in biotechnology have allowed the construction of a computer from DNA. One of the proposed solutions is a biomolecular finite automaton, a simple two-state DNA computer without memory, which was presented by Ehud Shapiro's group at the Weizmann Institute of Science. The main problem with this computer, in which biomolecules carry out logical operations, is its complexity - increasing the number of states of biomolecular automata. In this study, we constructed (in laboratory conditions) a six-state DNA computer that uses two endonucleases (e...
July 14, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28315853/effects-of-extracts-and-compounds-from-tricholoma-populinum-lange-on-degranulation-and-il-2-il-8-secretion-of-immune-cells
#13
Simon Merdivan, Kristina Jenett-Siems, Karsten Siems, Timo H J Niedermeyer, Nadin Schultze, Carmen Steinborn, Carsten Gründemann, Ulrike Lindequist
Tricholoma populinum Lange is an edible basidiomycete from the family Tricholomataceae. Extracts, fractions, and different metabolites isolated from the fruiting bodies of this mushroom were tested for degranulation-inhibiting activities on RBL-2H3 cells (rat basophils). Dichloromethane extracts decreased degranulation significantly, as did a fraction after column chromatography. In addition, the extract decreased the IL-2 release from Jurkat T cells and the release of IL-8 from HMC-1 human mast cells. The results show the significant effects of extracts of T...
July 14, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28306545/scent-gland-constituents-of-the-middle-american-burrowing-python-loxocemus-bicolor-serpentes-loxocemidae
#14
Thies Schulze, Paul J Weldon, Stefan Schulz
Analysis by gas chromatography/mass spectrometry of the scent gland secretions of male and female Middle American burrowing pythons (Loxocemus bicolor) revealed the presence of over 300 components including cholesterol, fatty acids, glyceryl monoalkyl ethers, and alcohols. The fatty acids, over 100 of which were identified, constitute most of the compounds in the secretions and show the greatest structural diversity. They include saturated and unsaturated, unbranched and mono-, di-, and trimethyl-branched compounds ranging in carbon-chain length from 13 to 24...
July 14, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28301324/response-of-alternative-splice-isoforms-of-osrad9-gene-from-oryza-sativa-to-environmental-stress
#15
Rui Li, Wenguo Wang, Fosheng Li, Qingwei Wang, Shenghua Wang, Ying Xu, Fang Chen
Rad9 protein plays an important role in cell-cycle checkpoint signal transduction in human and yeast cells, but knowledge about Rad9 in plants is limited. This study reports that the Rad9 gene of rice can generate the transcript products OsRad9.1 and OsRad9.2 through alternative splicing. OsRad9.1, with all nine exons, is the main cell-cycle checkpoint protein involved in the response of rice to genotoxic stresses (ultraviolet radiation and antibiotic stress), environmental stresses (drought, salt, and heavy metal stress), and auxin stimuli (2,4-D, IAA, and IBA)...
July 14, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28284041/the-atmyb12-activation-domain-maps-to-a-short-c-terminal-region-of-the-transcription-factor
#16
Ralf Stracke, Neslihan Turgut-Kara, Bernd Weisshaar
The Arabidopsis thaliana R2R3-MYB transcription factor MYB12 is a light-inducible, flavonol-specific activator of flavonoid biosynthesis. The transactivation activity of the AtMYB12 protein was analyzed using a C-terminal deletion series in a transient A. thaliana protoplast assay with the goal of mapping the activation domain (AD). Although the deletion of the last 46 C-terminal amino acids did not affect the activation capacity, the deletion of the last 98 amino acids almost totally abolished transactivation of two different target promoters...
July 14, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28258977/different-sensitivities-of-photosystem-ii-in-green-algae-and-cyanobacteria-to-phenylurea-and-phenol-type-herbicides-effect-on-electron-donor-side
#17
Ekaterina K Yotsova, Martin A Stefanov, Anelia G Dobrikova, Emilia L Apostolova
The effects of short-term treatment with phenylurea (DCMU, isoproturon) and phenol-type (ioxynil) herbicides on the green alga Chlorella kessleri and the cyanobacterium Synechocystis salina with different organizations of photosystem II (PSII) were investigated using pulse amplitude modulated (PAM) chlorophyll fluorescence and photosynthetic oxygen evolution measured by polarographic oxygen electrodes (Clark-type and Joliot-type). The photosynthetic oxygen evolution showed stronger inhibition than the PSII photochemistry...
July 14, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28063265/pegylation-potentiates-hepatoma-cell-targeted-liposome-mediated-in-vitro-gene-delivery-via-the-asialoglycoprotein-receptor
#18
Nkosiyethu K Mkhwanazi, Charles B de Koning, Willem A L van Otterlo, Mario Ariatti, Moganavelli Singh
Hepatocellular carcinoma is a burgeoning health issue in sub-Saharan Africa and East Asia where it is most prevalent. The search for gene medicine treatment modalities for this condition represents a novel departure from current treatment options and is gaining momentum. Here we report on nonPEGylated and on sterically stabilized PEGylated cationic liposomes decorated with D-galacto moieties linked to 24.1 Å spacers for asialoglycoprotein receptor (ASGP-R)-targeted vehiculation of pCMV-luc plasmid DNA. Cargo DNA is fully liposome associated at N/P ratio=3:1 and is partially protected from the effects of serum nucleases...
July 14, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28170343/bioenergetic-strategy-of-microalgae-for-the-biodegradation-of-tyrosol-and-hydroxytyrosol
#19
Aikaterini Papazi, Andreas Ioannou, Myrto Symeonidi, Andreas G Doulis, Kiriakos Kotzabasis
Olive mill wastewater has significant polluting properties due to its high phenolic content [mainly tyrosol (trs) and hydroxytyrosol (htrs)]. Growth kinetics and a series of fluorescence induction measurements for Scenedesmus obliquus cultures showed that microalgae can be tolerant of these phenolic compounds. Changes in the cellular energy reserves and concentration of the phenolic compounds adjust the "toxicity" of these compounds to the microalgae and are, therefore, the main parameters that affect biodegradation...
May 1, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28160544/effects-of-hypericum-perforatum-extract-on-oxaliplatin-induced-neurotoxicity-in-vitro-evaluations
#20
Lorenzo Cinci, Lorenzo Di Cesare Mannelli, Anna Maidecchi, Luisa Mattoli, Carla Ghelardini
Hypericum perforatum L. has been used for centuries as a natural remedy for the treatment of many disorders. Neuropathic pain is a common side effect of oxaliplatin-based chemotherapy and often the cause of therapy discontinuation. Thanks to its anti-inflammatory and analgesic effects, the use of H. perforatum may be a novel therapeutic strategy for neuropathy. The aim of this paper was to evaluate the effect of H. perforatum hydrophilic extract on an in vitro model of oxaliplatin-induced neurotoxicity. The antioxidant potential of extract was first evaluated in cell-free models by the thiobarbituric acid-reactive substances assay and nitro blue tetrazolium oxidation test; the ability of H...
May 1, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
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