Read by QxMD icon Read

Biomedical and Environmental Sciences: BES

Ni Wang, Yan Ma, Yu Hong Liu, Jian DU, Hui Zhang, Shi Heng Xie, Kun Zhu, Xiao Ya Lyu, Wei Shu, Hong Hong Wang, Guo Feng Zhu, Shou Yong Tan, Yan Yong Fu, Li Ping Ma, Lian Ying Zhang, Fei Ying Liu, Dai Yu Hu, Yan Ling Zhang, Xiang Qun Li, Liang Li
The objective of this prospective study of the risks of treatment failure in patients with drug-susceptible pulmonary tuberculosis (PTB) was to provide reference data to help develop a disease control strategy. Participants were recruited in eight provinces of China from October 2008 to December 2010. A total of 1447 patients with drug-susceptible PTB and older than 15 years of age were enrolled. Demographic characteristics, bacteriological test results, and patient outcome, i.e., cure or treatment failure were recorded and compared using the chi-square or Fisher's exact tests...
August 2016: Biomedical and Environmental Sciences: BES
Xin Ling Wang, Juan Song, Qin Qin Song, Jie Yu, Xiao Nuan Luo, Gui Zhen Wu, Jun Han
To understand the potential causes of laboratory-acquired infections and to provide possible solutions that would protect laboratory personnel, samples from a viral laboratory were screened to determine the main sources of contamination with six subtypes of Rhinovirus. Rhinovirus contamination was found in the gloves, cuffs of protective wear, inner surface of biological safety cabinet (BSC) windows, and trash handles. Remarkably, high contamination was found on the inner walls of the centrifuge and the inner surface of centrifuge tube casing in the rotor...
August 2016: Biomedical and Environmental Sciences: BES
Long Xing Fan, En Qi Wu, Jun Liu, Xiao Chen Qu, Chao Liu, Bao An Ning, Ying Liu
Plague is a virulent infectious disease in China. In this study, '3S' technology was used to perform spatial autocorrelation analysis and spatial interpolation analysis for Spermophilus dauricus (S. Dauricus, a species of ground squirrel) captured in Manchuria City in 2015. The results were visually inspected. During the two-month (May to July) plague surveillance in 2015, 198 S. dauricus individuals were captured in the study area in Manchuria City (48 monitoring areas) by using a day-by-day catching method...
August 2016: Biomedical and Environmental Sciences: BES
Mei Yuan, Yan Lyu, Su Ting Chen, Chao Cai, Yuan Li, Zhi Guo Zhang, Yun Xu Li, Ling Ling Dong, Yu Hong Fu, Hai Rong Huang, Ji Min Gao, Wei Min Li
We evaluate the performance of Xpert MTB/RIF for the diagnosis of extrapulmonary tuberculosis (EPTB) in China. The performance of Xpert was evaluated compared to the composite reference standard (CRS), drug susceptibility testing (DST), and imaging examination. The overall sensitivity and specificity of Xpert were 64.1% (195/304) and 100% (24/24), respectively, using CRS as the gold standard. The sensitivity was significantly higher than that of culture for pus (P<0.05). The proportion of EPTB-positive cases diagnosed by imaging was two times more than that diagnosed using Xpert; however, 6 out of 19 cases may have been overdiagnosed by imaging...
August 2016: Biomedical and Environmental Sciences: BES
Qian Zhou, Hai Feng Wu, Yuan Wei, Wen Li Zhu
We used Smo siRNA to inhibit hedgehog signaling pathway in embryonic day (E) 13 palatal shelves in organ culture. SiRNA 4 was chosen as the most efficient from four synthesized Smo siRNAs. Palatal shelf fusion rate of 4 μg/mL cyclopamine group was the lowest and significantly lower than that of blank control group (P<0.05), and that of siRNA 4 group was also lower than that of blank control group (P=0.183). At 48 h after transfection, Smo protein level of siRNA 4 group was 64.8% lower than that of blank control group (P<0...
August 2016: Biomedical and Environmental Sciences: BES
Zlatko Zimet, Marjan Bilban, Mateja Marc Malovrh, Peter Korošec, Borut Poljšak, Joško Osredkar, Mira Šilar
This study was to investigate whether working in conditions of elevated concentrations of mine gases (CO2, CO, CH4, DMS) and dust may result in oxidative stress. Coal miners (n=94) from the Velenje Coal mine who were arranged into control group and three groups according to a number of consecutive working days. 8-isoprostane as a biological marker of oxidative stress was measured in exhaled breath condensate (EBC). Miners who worked for three consecutive days had higher 8-isoprostane values in EBC compared to the control group...
August 2016: Biomedical and Environmental Sciences: BES
Nan Liu, Ling Xue, Yi Guan, Qing Zhao Li, Fu Yuan Cao, Shu Lan Pang, Wei Jun Guan
Silicosis is one of the most serious occupational diseases in China and dates back to centuries ago. In this study, we successfully established a rat model of silicosis by intratracheal silica injection for 28 days and determined hydroxyproline levels to evaluate collagen metabolism in lung homogenates. Oxidative stress status was evaluated by detecting catalase and glutathione peroxidase activities. Expression levels of peroxiredoxins (Prx I and Prx VI) were detected by Western blotting. Pulmonary surfactant protein A (SP-A) levels in rat serum and lung tissue were analyzed by ELISA, and SP-A and Prx expression levels in lung tissues were detected by immunohistochemistry...
August 2016: Biomedical and Environmental Sciences: BES
Yan Hua Kong, Qi Shi, Na Han, Ling Zhang, Yuan Yuan Zhang, Tong Xin Gao, Chen Chen, You Lin Li
OBJECTIVE: To investigate whether recuperating lung decoction (RLD) can modulate the composition of gut microbiota in rats during asthma treatment. METHODS: Fifteen Sprague-Dawley rats were divided randomly and equally into control group, model group, dexamethasone (DEX) group, RLD medium-dose group, and RLD high-dose group. The asthma model was established in all groups, except for the control group. The rats in the DEX and RLD groups were treated orally with DEX and RLD, respectively...
August 2016: Biomedical and Environmental Sciences: BES
Suni Lee, Hitoshi Okamoto, Shoko Yamamoto, Tamayo Hatayama, Hidenori Matsuzaki, Naoko Kumagai-Takei, Kei Yoshitome, Yasumitsu Nishimura, Toshiaki Sato, Yasuzo Kirita, Yoshio Fujii, Takemi Otsuki
OBJECTIVE: The custom-homebuilding company, Cosmic Garden Co. Ltd., located in Okayama City, Japan was established in 1997 and uses specific natural ore powder (SNOP) in wall materials and surveys customers in order to improve allergic symptoms. METHODS: To investigate the biological effects of SNOP, patients with a pollen allergy were recruited to stay in a room surrounded by cloth containing SNOP (CCSNOP), and their symptoms and various biological parameters were compared with those of individuals staying in a room surrounded by control non-woven cloth (NWC)...
August 2016: Biomedical and Environmental Sciences: BES
Zhu Liang, Qiao Yan Qiu, Jia Hui Wu, Jing Wen Zhou, Tian Xu, Ming Zhi Zhang, Yong Hong Zhang, Shao Yan Zhang
OBJECTIVE: No previous studies have evaluated the association between dyslipidemia, alcohol drinking, and diabetes in an Inner Mongolian population. We aimed to evaluate the co-effects of drinking and dyslipidemia on diabetes incidence in this population. METHODS: The present study was based on 1880 participants from a population-based prospective cohort study among Inner Mongolians living in China. Participants were classified into four subgroups according to their drinking status and dyslipidemia...
August 2016: Biomedical and Environmental Sciences: BES
Ying DU, Sheng Hua Yang, Sha Li, Chuan Jue Cui, Yan Zhang, Cheng Gang Zhu, Yuan Lin Guo, Na Qiong Wu, Ying Gao, Jing Sun, Qian Dong, Geng Liu, Jian Jun Li
OBJECTIVE: Very early-onset coronary artery disease (CAD) is a great challenge in cardiovascular medicine throughout the world, especially regarding its early diagnosis. This study explored whether circulating microRNAs (miRNAs) could be used as potential biomarkers for patients with very early-onset CAD. METHODS: We performed an initial screening of miRNA expression using RNA isolated from 20 patients with angiographically documented very early-onset CAD and 20 age- and sex-matched normal controls...
August 2016: Biomedical and Environmental Sciences: BES
Xiao Wei Shi, Bo Hui Shi, Ai Li Lyu, Feng Zhang, Tian Tian Zhou, Xiong Guo
To understand how differentially methylated genes (DMGs) might affect the pathogenesis of Kashin-Beck disease (KBD). Genome-wide methylation profiling of whole blood from 12 matched KBD and controls pairs was performed using a high-resolution Infinium 450 K methylation array. In total, 97 CpG sites were differentially methylated in KBD compared to the normal controls; of these sites, 36 sites were significantly hypermethylated (covering 22 genes) and 61 sites were significantly hypomethylated (covering 34 genes)...
July 2016: Biomedical and Environmental Sciences: BES
Huan He, Qing Zhen, Yong Li, Chang Gui Kou, Yu Chun Tao, Chang Wang, Joseph Sam Kanu, Yu Ping Lu, Ming Xi Yu, Hui Ping Zhang, Ya Qin Yu, Bo Li, Ya Wen Liu
Dyslipidemia is a risk factor for cardiovascular diseases (CVDs) in patients with diabetes, and non-high-density lipoprotein cholesterol (non-HDL-C) is a better predictor of CVDs than low-density lipoprotein cholesterol (LDL-C) in patients with diabetes. Therefore, we aimed to investigate the distribution of non-HDL-C and the prevalence of high non-HDL-C level in Chinese patients with diabetes mellitus and identify the associated risk factors. Non-HDL-C concentration positively correlated with total cholesterol, triglycerides, and LDL-C concentrations...
July 2016: Biomedical and Environmental Sciences: BES
Xiu Yuan He, Li Yun Yuan, Yong Tao Li, Meng Li, Yuan Chen, Hui Yuan, Jing Wu, Cheng Zhi Guo, Jing Li
The toxic effects of lead on normal rat kidney epithelial cells (NRK cells) may occur via various pathways. However, the role of intrinsic mitochondrial pathway in Lead-induced apoptosis in NRK cells has not been investigated. The purpose of our study was to investigate cytotoxic responses and cell apoptosis mediated by lead in NRK cells. NRK cells were treated with different concentrations of Lead acetate for 12 h to determine the cytotoxicity of lead. Mitochondrial transmembrane potential was also analyzed using a fluorescence spectrophotometer...
July 2016: Biomedical and Environmental Sciences: BES
Pi Wei Zhang, Chong Tian, Fang Yi Xu, Zhuo Chen, Raynard Burnside, Wei Jie Yi, Si Yun Xiang, Xiao Xie, Nan Nan Wu, Hui Yang, Na Na4 Zhao, Xiao Lei Ye, Chen Jiang1 Ying
Bovine aortic endothelial cells (BAECs) were cultured with high glucose (33 mmol/L), 4 mg/L green tea polyphenols (GTPs) or 4 mg/L GTPs co-treatment with high glucose for 24 h in the presence or absence of Bafilomycin-A1 (BAF). We observed that high glucose increased the accumulation of LC3-II. Treatment with BAF did not further increase the accumulation of LC3-II. Results also showed an increased level of p62 and decreased Beclin-1. However, GTPs showed inversed trends of those proteins. Furthermore, GTPs co-treatment with high glucose decreased the level of LC3-II and a much higher accumulation of LC3-II was observed in the presence of BAF in comparison with high glucose alone...
July 2016: Biomedical and Environmental Sciences: BES
Na Sun, Yong Jun Wen, Shu Qin Zhang, Hong Wei Zhu, Li Guo, Feng Xue Wang, Qiang Chen, Hong Xia Ma, Shi Peng Cheng
There were 4 Acinetobacter lwoffii obtained from soil samples. The antimicrobial susceptibility of the strains to 16 antimicrobial agents was investigated using K-B method. Three isolates showed the multi-drug resistance. The presence of resistance genes and integrons was determined using PCR. The aadA1, aac(3')-IIc, aph(3')-VII, aac(6')-Ib, sul2, cat2, floR, and tet(K) genes were detected, respectively. Three class 1 integrons were obtained. The arr-3-aacA4 and blaPSE-1 gene cassette, which cause resistance to aminoglycoside and beta-lactamase antibiotics...
July 2016: Biomedical and Environmental Sciences: BES
Mohammad Naderi, Mohammad Hashemi, Gholamreza Bahari
OBJECTIVE: The effect of rs4331426 polymorphism in the Chr18q11.2 locus on pulmonary tuberculosis (PTB) risk was evaluated. METHODS: This case-control study included 208 PTB patients and 204 healthy subjects. Genotyping of the rs4331426 variant was conducted using polymerase chain reaction restriction fragment length polymorphism. RESULTS: The frequencies of genotypes AA, AG, and GG polymorphisms were 83.1%, 15.9%, and 1.0% in the PTB group and 84...
July 2016: Biomedical and Environmental Sciences: BES
Jun Mei Zhou, Ming Zhang, Shu Wang, Bing Yuan Wang, Cheng Yi Han, Yong Cheng Ren, Lu Zhang, Hong Yan Zhang, Xiang Yu Yang, Yang Zhao, Dong Sheng Hu
OBJECTIVE: This meta-analysis was performed to summarize the association of the ADIPOQ rs2241766 and rs266729 polymorphisms with metabolic syndrome (MS) in the Chinese population. METHODS: We searched for articles in MEDLINE via PubMed, EMBASE, HuGE Navigator, CNKI, and Wanfang databases and calculated odds ratios (ORs) with 95% confidence intervals (CIs) to determine the strength of associations in fixed- or random-effects models. RESULTS: We included 21 articles in the meta-analysis: 17 reports of ADIPOQ rs2241766 with 3628 cases and 3000 controls and 8 of rs266729 with 2021 cases and 2226 controls...
July 2016: Biomedical and Environmental Sciences: BES
Zhun Yuan, Xian Ping Ying, Wei Jian Zhong, Shi Min Tian, Yu Wang, Yong Rui Jia, Wen Chen, Juan Ling Fu, Peng Zhao, Zong Can Zhou
OBJECTIVE: To investigate the role of autophagy in MnCl2-induced apoptosis in human bronchial epithelial 16HBE cells. METHODS: Cell proliferation was measured by MTT assay. Mitochondrial membrane potential (MMP) and apoptosis were measured by flow cytometry. Autophagic vacuoles were detected by fluorescence microscopy. Cellular levels of apoptosis and autophagy-related proteins were measured by western blotting. RESULTS: 16HBE cell proliferation was inhibited by MnCl2 in a dose- and time-dependent manner...
July 2016: Biomedical and Environmental Sciences: BES
Xu Rui Zhang, Yong Ai Liu, Fang Sun, He Li, Su Wen Lei, Ju Fang Wang
OBJECTIVE: To explore the role of p21 in ionizing radiation-induced changes in protein levels during the G2/M transition and long-term G2 arrest. METHODS: Protein expression levels were assessed by western blot in the human uveal melanoma 92-1 cells after treatment with ionizing radiation. Depletion of p21 was carried out by employing the siRNA technique. Cell cycle distribution was determined by flow cytometry combined with histone H3 phosphorylation at Ser28, an M-phase marker...
July 2016: Biomedical and Environmental Sciences: BES
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"