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Biomedical and Environmental Sciences: BES

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https://www.readbyqxmd.com/read/28807103/research-progress-in-occupational-health-risk-assessment-methods-in-china
#1
Li Fang Zhou, Fang Tian, Huag Zou, Wei Ming Yuan, Mo Hao, Mei Bian Zhang
No abstract text is available yet for this article.
August 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28807102/bitter-melon-powder-protects-against-obesity-associated-fatty-liver-disease-by-improving-colonic-microenvironment-in-rats-with-high-fat-diet-induced-obesity
#2
Juan Bai, Ying Zhu, Ying Dong
This study explored how bitter melon powder (BMP) alters the colonic microenvironment during the development of obesity-associated fatty liver in rats. We observed that BMP effectively inhibited the body weight gain and lipid accumulation in the liver, ameliorated glucose intolerance, and increased the colon weight after an 8-week treatment compared to that in the high-fat diet (HFD) group. BMP significantly decreased fecal water toxicity towards HT-29 cells, as revealed by the cell counting kit (CCK)-8 assay results, and the mRNA expression of Toll-like receptor 4 (TLR4) in colon mucosa...
August 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28807101/examine-the-correlation-between-heat-shock-protein-ibpa-and-heat-tolerance-in-cronobacter-sakazakii
#3
Zhi Jing Zhao, Bin Wang, Jing Yuan, Hao Yu Liang, Si Guo Dong, Ming Zeng
We used a proteomic approach to identify IbpA in Cronobacter sakazakii (C. sakazaki), which is related to heat tolerance in this strain. The abundance of IbpA in C. sakazakii strains strongly increased after heat shock. C. sakazakii CMCC 45402 ibpA deletion mutants were successfully constructed. The C. sakazakii CMCC 45402 ΔibpA and wild-type strains could not be distinguished based on colony morphology on LB agar plates or biochemical assays. The growth of the C. sakazakii CMCC 45402 ΔibpA mutant in heat shock conditions was indistinguishable from that of the isogenic wild-type, but showed greater heat resistance than E...
August 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28807100/two-cases-of-multi-antibiotic-resistant-cronobacter-spp-infections-of-infants-in-china
#4
Jing Hua Cui, Bo Yu, Yun Xiang, Zhen Zhang, Ting Zhang, Ying Chun Zeng, Zhi Gang Cui, Xi Xiang Huo
Infections by Cronobacter spp. are hazardous to infants since they can lead to neonatal meningitis, bacteremia, and necrotizing enterocolitis. Cronobacter spp. are frequently resistant to β-lactam derivatives, macrolides, and aminoglycosides. In addition, multi-resistant strains have also been detected. In China, the isolation rate of Cronobacter spp. from commercial powdered infant formula (PIF) or follow-up formula (FUF) is relatively high. Nevertheless, clinical cases of Cronobacter infection have been ignored to date...
August 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28807099/role-of-endoplasmic-reticulum-stress-in-silica-induced-apoptosis-in-raw264-7-cells
#5
Yong Bin Hu, Xia Wu, Xiao Feng Qin, Lei Wang, Pin Hua Pan
OBJECTIVE: We investigated the role of endoplasmic reticulum stress (ERS) in silica-induced apoptosis in alveolar macrophages in vitro. METHODS: RAW264.7 cells were incubated with 200 μg/mL silica for different time periods. Cell viability was assayed by the MTT assay. Cell apoptosis was evaluated by DAPI staining, flow cytometry analysis, and Western blot analysis of caspase-3. Morphological changes in the endoplasmic reticulum were observed by transmission electron microscopy...
August 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28807098/protective-effect-of-ozone-against-hemiscorpius-lepturus-envenomation-in-mice
#6
Parvaneh Naserzadeh, Farshad Shahi, Delavar Shahbazzadeh, Mostafa Ghanei, Khadijeh Ashtari, Yoones Panahi, Mir-Jamal Hosseini, Morteza Izadi
OBJECTIVE: Scorpion (Hemiscorpius lepturus) stings are a public health concern in Iran, particularly in south and southwestern regions of Iran. The gold standard for the treatment of a scorpion sting is anti-venom therapy. However, immunotherapy can have serious side effects, such as anaphylactic shock (which can sometimes even lead to death). The aim of the current study was to demonstrate the protective effect of ozone against toxicity induced by Hemiscorpius lepturus (H. lepturus) venom in mice...
August 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28807097/genotypic-characterization-of-methicillin-resistant-staphylococcus-aureus-isolated-from-pigs-and-retail-foods-in-china
#7
Wei Wang, Feng Liu, Zulqarnain Baloch, Cun Shan Zhang, Ke Ma, Zi Xin Peng, Shao Fei Yan, Yu Jie Hu, Xin Gan, Yin Ping Dong, Yao Bai, Feng Qin Li, Xiao Mein Yan, Ai Guo Ma, Jin Xu
OBJECTIVE: To investigate the genotypic diversity of Methicillin-resistant Staphylococcus aureus (MRSA) isolated from pigs and retail foods from different geographical areas in China and further to study the routes and rates of transmission of this pathogen from animals to food. METHODS: Seventy-one MRSA isolates were obtained from pigs and retail foods and then characterized by multi-locus sequencing typing (MLST), spa typing, multiple-locus variable number of tandem repeat analysis (MLVA), pulsed-field gel electrophoresis (PFGE), and antimicrobial susceptibility testing...
August 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28807096/a-five-year-surveillance-of-carbapenemase-producing-klebsiella-pneumoniae-in-a-pediatric-hospital-in-china-reveals-increased-predominance-of-ndm-1
#8
Fang Dong, Jie Lu, Yan Wang, Jin Shi, Jing Hui Zhen, Ping Chu, Yang Zhen, Shu Jing Han, Yong Li Guo, Wen Qi Song
OBJECTIVE: To characterize carbapenem (CPM)-non-susceptible Klebsiella pneumoniae (K. pneumoniae) and carbape-nemase produced by these strains isolated from Beijing Children's Hospital based on a five-year surveillance. METHODS: The Minimal Inhibition Concentration values for 15 antibiotics were assessed using the Phonix100 compact system. PCR amplification and DNA sequencing were used to detect genes encoding carbapenemases. WHONET 5.6 was finally used for resistance analysis...
August 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28807095/simultaneous-detection-of-13-key-bacterial-respiratory-pathogens-by-combination-of-multiplex-pcr-and-capillary-electrophoresis
#9
Lu Xi Jiang, Hong Yu Ren, Hai Jian Zhou, Si Hong Zhao, Bo Yan Hou, Jian Ping Yan, Tian Qin, Yu Chen
OBJECTIVE: Lower respiratory tract infections continue to pose a significant threat to human health. It is important to accurately and rapidly detect respiratory bacteria. To compensate for the limits of current respiratory bacteria detection methods, we developed a combination of multiplex polymerase chain reaction (PCR) and capillary electrophoresis (MPCE) assay to detect thirteen bacterial pathogens responsible for lower respiratory tract infections, including Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Mycoplasma pneumoniae, Legionella spp...
August 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28756816/epidemiological-characteristics-and-risk-factors-of-the-pertussis-in-infants-12-months-of-age-in-tianjin-china
#10
Hai Tao Huang, Zhi Gang Gao, Yong Liu, Li Juan Wang, Yan Ping Liu, Ying Zhang
The epidemiologic characteristics of 631 infant pertussis cases < 12 months, which accounted for 57.42% of the total cases, were analyzed by descriptive epidemiology in Tianjin, between 2010 and 2015. The incidence of infants was 104.66/100,000, which was 118 times higher than in other age groups (P < 0.001). The primary type of pertussis simultaneously presented in households was adult-to-infant (70.51%). The parents were identified as the source of infection in 80.18% of cases for infants. The positive rate of placental antibody transfer was 31...
July 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28756815/establishment-of-a-predictive-diagnostic-model-for-acute-mycoplasma-pneumoniae-infection-in-elderly-patients-with-community-acquired-pneumonia
#11
Hong Li Xiao, De Li Xin, Yan Wang, Li Jian Cui, Xiao Ya Liu, Song Liu, Li Hong Song, Chun Ling Liu, Cheng Hong Yin
We established a diagnostic model to predict acute Mycoplasma pneumoniae (M. pneumonia) infection in elderly Community-acquired pneumonia (CAP) patients. We divided 456 patients into acute and non-acute M. pneumoniae infection groups. Binary logistic regression and receiver operating characteristic (ROC) curves were used to establish a predictive model. The following independent factors were identified: age ⋝ 70 years; serum cTNT level ⋝ 0.05 ng/mL; lobar consolidation; mediastinal lymphadenopathy; and antibody titer in the acute phase ⋝ 1:40...
July 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28756814/grape-seed-procyanidin-extract-reduces-arsenic-induced-renal-inflammatory-injury-in-male-mice
#12
Cheng Wang, Jun Li, Guan Ling Song, Qiang Niu, Shang Zhi Xu, Gang Ling Feng, Hai Xia Wang, Yu Li, Shu Gang Li, Feng Li
The aim of the present study is to evaluate the ability and mechanism by which grape seed procyanidin extract (GSPE) relieves arsenic trioxide (As2O3)-induced renal inflammatory injury. Therefore, male Kunming mice were treated with As2O3 and/or GSPE by gavage for 5 weeks. Mice were then sacrificed and inflammatory cytokines of kidneys were examined by ELISA, whereas the expression levels of molecules involved in the nuclear factor (NF)-κB signaling pathway were evaluated by both qRT-PCR and Western blot. Our results indicate that GSPE prevents As2O3-mediated renal inflammatory injury by inhibiting activation of the NF-κB signaling pathway and inflammatory cytokine production, while promoting expression of anti-inflammatory cytokines...
July 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28756813/heavy-metal-assessment-among-chinese-nonferrous-metal-exposed-workers-from-the-jinchang-cohort-study
#13
Ai Min Yang, Zhi Yuan Cheng, Hong Quan Pu, Ning Cheng, Hai Yan Li, Si Min Liu, Jiao Ding, Juan Sheng Li, Xiao Bin Hu, Xiao Wei Ren, Tong Zhang Zheng, Ya Na Bai
Environmental exposure to heavy metals has been linked to a wide range of human health hazards. We detected the levels of 15 metals in urine samples from 500 representative sub-samples in an ongoing occupational cohort study (Jinchang Cohort) to directly evaluate metal exposure levels. Fifteen metals, namely As, Ba, Be, Cd, Cs, Cr, Co, Cu, Pb, Mn, Ni, Se, Tl, U, and Zn, were detected by inductively coupled plasma quadruple mass spectrometry. The results showed that median creatinine adjustment and geometric mean urinary metal levels were higher in the heavy metal-exposed group, except Se and Zn, than other reported general or occupational populations...
July 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28756812/dog-transmitted-rabies-in-beijing-china
#14
Jing Yuan Zhang, Bi Zhang, Shou Feng Zhang, Fei Zhang, Nan Li, Ye Liu, Rong Liang Hu
Rabies remains a continuous threat to public health in Beijing. In this study, a total of 224 brain tissues were collected from suspected infected stray dogs within Beijing between January 2015 and December 2016. Among them, total of 67 samples were diagnosed positive for rabies. In the phylogenetic analysis, rabies in Beijing is currently a relatively independent public health issue originating from local rabid dogs apart from the imported cases from elsewhere in the country. Because vaccination of unregistered dogs against rabies is still neglected in Beijing and other regions of China, national and local authorities should play central roles in all related aspects, such as development of policies, engagement of stakeholders for public and professional education, entire vaccination process, and animal management...
July 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28756811/comparing-the-genotype-and-drug-susceptibilities-between-mycobacterium-avium-and-mycobacterium-intracellulare-in-china
#15
Hui Wen Zheng, Yu Pang, Guang Xue He, Yuan Yuan Song, Yan Lin Zhao
OBJECTIVE: Mycobacterium avium (M. avium) and Mycobacterium intracellulare (M. intracellulare) are the major causative agents of nontuberculous mycobacteria (NTM)-related pulmonary infections. However, little is known about the differences in drug susceptibility profiles between these two species. METHODS: A total of 393 NTM isolates were collected from Shanghai Pulmonary Disease Hospital. Sequencing of partial genes was performed to identify the strains at species level...
July 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28756810/serum-thyroglobulin-a-sensitive-biomarker-of-iodine-nutrition-status-and-affected-by-thyroid-abnormalities-and-disease-in-adult-populations
#16
Yang DU, Yan Hui Gao, Zhuo Ying Feng, Fan Gang Meng, Li Jun Fan, Dian Jun Sun
OBJECTIVE: To evaluate the usefulness of the thyroglobulin (Tg) level in adults as a nutritional biomarker of iodine status and to identify the factors related to the serum Tg level. METHODS: A cross-sectional study was conducted in adult populations of areas differing in iodine nutrition from three provinces (Autonomous Region) in China. Serum levels of thyroid hormones and Tg as well as thyroid autoantibodies were measured. The thyroid volume and nodule were measured by ultrasound...
July 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28756809/first-report-in-china-on-the-identification-and-drug-sensitivity-of-mycobacterium-elephantis-isolated-from-the-milk-of-a-cow-with-mastitis
#17
Ling Yun Ji, Dong Lei Xu, Shu Peng Yin, Hai Can Liu, Gui Lian Li, Yi Jiang, Jian Hao Wei, Hao Zeng, Yong Liang Lou, Jian Xin Lyu, Kang Lin Wan
OBJECTIVE: In this study, milk from a cow with mastitis was analyzed to determine the presence of mycobacterial infection. Milk quality and security problems pertaining to the safe consumption of dairy products were also discussed in this study. METHODS: Milk was preprocessed with 4% NaOH. Then, mycobacteria were isolated from the milk sample on L-J medium. The isolate was identified using multiple loci Polymerase Chain Reaction (PCR) and multi-locus sequence analysis with 16S rRNA, sodA, hsp65, and ITS genes...
July 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28756808/role-of-immune-microenvironmental-factors-for-improving-the-ipi-related-risk-stratification-of-aggressive-b-cell-lymphoma
#18
Yi Gong, Rui Chen, Xi Zhang, Zhong Min Zou, Xing Hua Chen
OBJECTIVE: To investigate the risk stratification of aggressive B cell lymphoma using the immune microenvironment and clinical factors. METHODS: A total of 127 patients with aggressive B cell lymphoma between 2014 and 2015 were enrolled in this study. CD4, Foxp3, CD8, CD68, CD163, PD-1, and PD-L1 expression levels were evaluated in paraffin-embedded lymphoma tissues to identify their roles in the risk stratification. Eleven factors were identified for further evaluation using analysis of variance, chi-square, and multinomial logistic regression analysis...
July 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28756807/association-between-plasma-metal-levels-and-diabetes-risk-a-case-control-study-in-china
#19
Xiu Ting Li, Peng Fei Yu, Yan Gao, Wen Hui Guo, Jun Wang, Xin Liu, Ai Hua Gu, Gui Xiang Ji, Qiu Dong, Bo Shen Wang, Ying Cao, Bao Li Zhu, Hang Xiao
OBJECTIVE: Many metals, some of which have been classified as environmental endocrine disruptors, are used extensively in everyday consumer products and are ubiquitous in our living environment. In the present study, we aimed to explore the associations between the prevalence risk of type 2 diabetes and plasma levels of 20 trace elements as well as those of heavy metals in a Han Chinese population. METHODS: We conducted a case-control study to investigate the associations between plasma concentrations of 20 metals and diabetes in Jiangsu province...
July 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28756806/erk1-2-mediated-cytoplasmic-accumulation-of-hnrnpk-antagonizes-trail-induced-apoptosis-through-upregulation-of-xiap-in-h1299-cells
#20
Wen Si Huang, Feng Mei Xu, Qing Zhong Zeng, Xiao Hui Liu, Xue Juan Gao, Lang Xia Liu
OBJECTIVE: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) resistance greatly limits the clinical therapeutic efficacy of TRAIL. Elucidating the molecular mechanism underlying TRAIL resistance will be fundamental to resolving this problem. METHODS: Nuclear and cytoplasmic protein extraction and immuno?uorescence (IF) assay were used to detect changes in heterogeneous nuclear ribonucleoprotein K (hnRNPK) localization in H1299 cells. The evaluation of cell apoptosis in cells transfected with GFP-hnRNPK, GFP-hnRNPK S284/353A, or GFP-hnRNPK S284/353D mutant was performed using cleaved caspase-3 antibody...
July 2017: Biomedical and Environmental Sciences: BES
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