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Research in Microbiology

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https://www.readbyqxmd.com/read/28774774/saccharomyces-cerevisiae-ktr4-ktr5-and-ktr7-encode-mannosyltransferases-differentially-involved-in-the-n-and-o-linked-glycosylation-pathways
#1
Nahúm V Hernández, Luz A López-Ramírez, Diana F Díaz-Jiménez, Erika Mellado-Mojica, Iván Martínez-Duncker, Mercedes G López, Héctor M Mora-Montes
Saccharomyces cerevisiae is a model to understand basic aspects of protein glycosylation pathways. Although these metabolic routes have been thoroughly studied, there are still knowledge gaps; among them, the role of the MNT1/KRE2 gene family. This family is composed of nine members, with only six functionally characterized. The enzymes Ktr1, Ktr3, and Mnt1/Kre2 have overlapping activities in both O-linked and N-linked glycan synthesis; while Ktr2 and Yur1 participate exclusively in the elongation of the N-linked glycan outer chain...
July 31, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28728853/eve-s-garden-myths-legends-and-secrets-unmasked
#2
Tania Crucitti
This special issue of Research in Microbiology aims to provide some insights in one of women's most fascinating interacting microbial communities: the vaginal microbiome. Even in the 21st century, with many advanced methods at hand, the definition of the healthy vaginal microbiome remains under debate. The interest in studying the dysbiosis of the vaginal microbiome is increasing and goes well beyond the study of bacterial vaginosis. The selection of contributions in this issue summarizes what is currently known in terms of inter- and intra-microorganisms interaction as well as the virulence factors that some of them may deploy...
July 17, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28728852/in%C3%A2-vitro-characteristics-of-an-atlantic-salmon-salmo-salar-l-hind-gut-microbial-community-in-relation-to-different-dietary-treatments
#3
Kamarul Zaman Zarkasi, Richard S Taylor, Brett D Glencross, Guy C J Abell, Mark L Tamplin, John P Bowman
In this study, microbial community dynamics were assessed within a simple in vitro model system in order to understand those changes influenced by diet. The abundance and diversity of bacteria were monitored within different treatment slurries inoculated with salmon faecal samples in order to mimic the effects of dietary variables. A total of five complete diets and two ingredients (plant meal) were tested. The total viable counts (TVCs) and sequencing data revealed that there was very clear separation between the complete diets and the plant meal treatments, suggesting a dynamic response by the allochthonous bacteria to the treatments...
July 17, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28716397/polymicrobial-infections-and-biofilms-in-women-s-health-gahro-expert-group-meeting-report-2017
#4
EDITORIAL
Nuno Cerca, Mario Vaneechoutte, Alexander Guschin, Alexander Swidsinski
No abstract text is available yet for this article.
July 14, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28709697/effects-of-p-limitation-and-molecules-from-peanut-root-exudates-on-pqqe-gene-expression-and-pqq-promoter-activity-in-the-phosphate-solubilizing-strain-serratia-sp-s119
#5
Liliana M Ludueña, Maria S Anzuay, Cynthia Magallanes-Noguera, Maria L Tonelli, Fernando J Ibañez, Jorge G Angelini, Adriana Fabra, Matthew McIntosh, Tania Taurian
The mineral phosphate-solubilizing phenotype in bacteria is attributed predominantly to secretion of gluconic acid produced by oxidation of glucose by the glucose dehydrogenase enzyme and its cofactor, pyrroloquinoline quinone. This study analyzes pqqE gene expression and pqq promoter activity in the native phosphate-solubilizing bacterium Serratia sp S119 growing under P-limitation, and in the presence of root exudates obtained from peanut plants, also growing under P-limitation. Results indicated that Serratia sp...
July 11, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28684253/sub-inhibitory-concentrations-of-gentamicin-triggers-the-expression-of-aac-6-ie-aph-2%C3%A2-ia-chaperons-and-biofilm-related-genes-in-lactobacillus-plantarum-mcc-3011
#6
Jaimee George, Prakash Motiram Halami
The study aimed to analyze the effects of sub-inhibitory concentrations of gentamicin on the expressions of high level aminoglycoside resistant (HLAR) bifunctional aac(6')-Ie-aph(2″)Ia, biofilm and chaperone genes in Lactobacillus plantarum. The analysis of the biofilm formation in five isolates obtained from chicken sausages indicated their role in exhibiting phenotypic resistance based on the varied MIC values despite carrying the bifunctional gene. The biofilm formation significantly increased when L. plantarum MCC 3011 was grown in sub-inhibitory concentrations of gentamicin (4 μg/ml), kanamycin (8 μg/ml) and streptomycin (2 μg/ml)...
July 4, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28629869/evaluation-of-cell-wall-damage-by-dimethyl-sulfoxide-in-candida-species
#7
María Cristina León-García, Emmanuel Ríos-Castro, Everardo López-Romero, Mayra Cuéllar-Cruz
Studies dealing with the response of microorganisms to oxidative stress require the dissolution of oxidant agents in an appropriate solvent. A commonly used medium is dimethyl sulfoxide, which has been considered as an innocuous polar solvent. However, we have observed significant differences between control, untreated cells and those receiving increasing amounts of the oxidant and hence increasing amounts of DMSO, to the maximum allowed of 1%. Here we show that, while this solvent does not influence yeast cell viability, it does affect expression of cell wall proteins as well as catalase activity...
June 17, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28576703/porin-flexibility-in-providencia-stuartii-cell-surface-exposed-loops-l5-and-l7-are-markers-of-providencia-porin-omppst1
#8
Que-Tien Tran, Laure Maigre, Tommaso D'Agostino, Matteo Ceccarelli, Mathias Winterhalter, Jean-Marie Pagès, Anne Davin-Regli
Epidemiologically unrelated Providencia stuartii strains isolated in hospitals in the south of France were investigated for their porin sequences and profiles. Noticeable resistance to β-lactams was found to be associated with production of extended spectrum β-lactamases or AmpC overproduction, but not metallo-β-lactamases. At the same time, the expression level of outer membrane porins was unmodified in these isolates. The identity of the amino acid sequences of the major porin OmpPst1 was less than 90% in the tested clinical strains, whereas sequences of the second major porin OmpPst2 were found to be identical in all isolates...
May 30, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28366837/slpe-is-a-calcium-dependent-cytotoxic-metalloprotease-associated-with-clinical-isolates-of-serratia-marcescens
#9
Nicholas A Stella, Jake D Callaghan, Liang Zhang, Kimberly M Brothers, Regis P Kowalski, Jean J Huang, Patrick H Thibodeau, Robert M Q Shanks
Serralysin-like proteases are found in a wide variety of bacteria. These metalloproteases are frequently implicated in virulence and are members of the widely conserved RTX-toxin family. We identified a serralysin-like protease in the genome of a clinical isolate of Serratia marcescens that is highly similar to the canonical serralysin protein, PrtS. This gene was named serralysin-like protease E, SlpE, and was found in the majority (67%) of tested clinical isolates, but was absent from most tested non-clinical isolates including the insect pathogen and reference S...
July 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28263907/role-of-the-interplay-between-quorum-sensing-regulator-vqsr-and-the-pseudomonas-quinolone-signal-in-mediating-carbapenem-tolerance-in-pseudomonas-aeruginosa
#10
Darija Viducic, Keiji Murakami, Takashi Amoh, Tsuneko Ono, Yoichiro Miyake
Pseudomonas aeruginosa coordinates its response to environmental conditions through activation of a quorum sensing (QS) system. In this study, we investigated the regulatory interaction between the QS transcriptional regulator VqsR and the Pseudomonas quinolone signal (PQS) through integration of sigma factor RpoS, and we addressed whether one of the pathways controlling carbapenem tolerance can be attributed to VqsR. We demonstrate that vqsR expression at the transcriptional level is regulated by pqsA, pqsR, and pqsE...
June 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28263906/characterization-of-genetic-determinants-involved-in-antibiotic-resistance-in-aeromonas-spp-and-fecal-coliforms-isolated-from-different-aquatic-environments
#11
Alessandro Carnelli, Federica Mauri, Antonella Demarta
Aeromonas spp. and fecal coliforms, two abundant and cultivable bacterial populations that can be found in water ecosystems, might substantially contribute to the spread of antibiotic resistance. We investigated the presence and spread of transposons (elements that can move from one location to another in the genome), integrons (structures able to capture and incorporate gene cassettes) and resistance plasmids in strains isolated from polluted and unpolluted water. We recovered 231 Aeromonas and 250 fecal coliforms from water samplings with different degrees of pollution (hospital sewage, activated sludge of a wastewater treatment plant, river water before and after treatment and water from an alpine lake)...
June 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28263905/carotenoid-production-and-phenotypic-variation-in-azospirillum-brasilense
#12
Gal Reem Brenholtz, Dafna Tamir-Ariel, Yaacov Okon, Saul Burdman
We assessed the occurrence of phenotypic variation in Azospirillum brasilense strains Sp7, Cd, Sp245, Az39 and phv2 during growth in rich media, screening for variants altered in colony pigmentation or extracellular polysaccharide (EPS) production. Previous studies showed that EPS-overproducing variants of Sp7 appear frequently following starvation or growth in minimal medium. In contrast, no such variants were detected during growth in rich media in the tested strains except for few variants of phv2. Regarding alteration in colony pigmentation (from pink to white in strain Cd and from white to pink in the others), strain Sp7 showed a relatively high frequency of variation (0...
June 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28263904/utilization-of-interferometric-light-microscopy-for-the-rapid-analysis-of-virus-abundance-in-a-river
#13
Céline Roose-Amsaleg, Yasmina Fedala, Catherine Vénien-Bryan, Josette Garnier, Albert-Claude Boccara, Martine Boccara
There is a constant need for direct counting of biotic nanoparticles such as viruses to unravel river functioning. We used, for the first time in freshwater, a new method based on interferometry differentiating viruses from other particles such as membrane vesicles. In the French Marne River, viruses represented between 42 and 72% of the particles. A spring monitoring in 2014 revealed their increase (2.1 × 10(7) to 2.1 × 10(8) mL(-1)) linked to an increase in algal biomass and diversity of bacterial plankton...
June 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28235561/physiological-and-comparative-genomic-analysis-of-acidithiobacillus-ferrivorans-pq33-provides-psychrotolerant-fitness-evidence-for-oxidation-at-low-temperature
#14
Robert Ccorahua-Santo, Anika Eca, Michel Abanto, Gregory Guerra, Pablo Ramírez
Friendly environmental hydrometallurgy at low temperatures is principally promoted by Acidithiobacillus ferrivorans. Until recently, the synergy between cold tolerance and the molecular mechanism of ferrous iron (Fe(2+)) oxidation was unknown. In the present paper, we conducted a physiological and comparative genomics analysis of the new strain A. ferrivorans PQ33 to elucidate the oxidation mechanism at low temperatures, with emphasis placed on trehalose and the Rus operon. PQ33 exhibited a doubling time of 66...
June 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28214542/several-hpept1-transported-drugs-are-substrates-of-the-escherichia-coli-proton-coupled-oligopeptide-transporter-ydgr
#15
Bala K Prabhala, Nanda G Aduri, Mazhar Iqbal, Moazur Rahman, Michael Gajhede, Paul R Hansen, Osman Mirza
Proton-dependent oligopeptide transporters (POTs) are secondary active transporters found in all kingdoms of life. POTs utilize the proton electrochemical gradient for the uptake of nutrient dipeptides and tripeptides. The human POT hPepT1 is known to transport a number of drugs. As part of ongoing studies on substrate specificities of POTs from Escherichia coli, our aim in this study was to investigate whether bacterial POTs could also transport these drugs. For this, we selected the common orally administered drugs sulpiride, bestatin, valacyclovir, ampicillin and oseltamivir, that are all transported by hPepT1...
June 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28213104/isolation-and-characterization-of-a-virulent-bacteriophage-infecting-acinetobacter-johnsonii-from-activated-sludge
#16
Niansi Fan, Rong Qi, Min Yang
A double-stranded DNA phage named AJO1, infecting Acinetobacter johnsonii, which plays an important role in wastewater treatment, was isolated from activated sludge in a full-scale municipal wastewater treatment plant. Based on morphological taxonomy, AJO1, with an icosahedral head 55 ± 2 nm in diameter and a non-contractile tail 8 ± 2 nm in length, was classified as a member of the Podoviridae family. Bacterial infection characteristics were as follows: no polyvalent infectivity, optimal multiplicity of infection of 10(-2); eclipse and burst size of 30 min and 51...
June 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28161485/identification-and-characterization-of-the-genes-encoding-carbon-monoxide-dehydrogenase-in-terrabacter-carboxydivorans
#17
Jae Ho Lee, Sae Woong Park, Young Min Kim, Jeong-Il Oh
Terrabacter carboxydivorans is able to grow aerobically at low concentrations of carbon monoxide (CO) as a sole source of carbon and energy. The genes for carbon monoxide dehydrogenase (CO-DH) were cloned from T. carboxydivorans and analyzed. The operon encoding T. carboxydivorans CO-DH was composed of three structural genes with the transcriptional order of cutB, cutC and cutA, as well as an additional accessory gene (orf4). Phylogenetic analysis of CutA revealed that T. carboxydivorans CO-DH was classified into a group distinct from previously characterized CO-DHs...
June 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28131895/biofilm-formation-and-cellulose-expression-by-bordetella-avium-197n-the-causative-agent-of-bordetellosis-in-birds-and-an-opportunistic-respiratory-pathogen-in-humans
#18
Kimberley McLaughlin, Ayorinde O Folorunso, Yusuf Y Deeni, Dona Foster, Oksana Gorbatiuk, Simona M Hapca, Corinna Immoor, Anna Koza, Ibrahim U Mohammed, Olena Moshynets, Sergii Rogalsky, Kamil Zawadzki, Andrew J Spiers
Although bacterial cellulose synthase (bcs) operons are widespread within the Proteobacteria phylum, subunits required for the partial-acetylation of the polymer appear to be restricted to a few γ-group soil, plant-associated and phytopathogenic pseudomonads, including Pseudomonas fluorescens SBW25 and several Pseudomonas syringae pathovars. However, a bcs operon with acetylation subunits has also been annotated in the unrelated β-group respiratory pathogen, Bordetella avium 197N. Our comparison of subunit protein sequences and GC content analyses confirms the close similarity between the B...
June 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28111289/ancient-highly-conserved-proteins-from-a-luca-with-complex-cell-biology-provide-evidence-in-support-of-the-nuclear-compartment-commonality-nucom-hypothesis
#19
REVIEW
James T Staley, John A Fuerst
The nuclear compartment commonality (NuCom) hypothesis posits a complex last common ancestor (LUCA) with membranous compartments including a nuclear membrane. Such a LUCA then evolved to produce two nucleated lineages of the tree of life: the Planctomycetes-Verrucomicrobia-Chlamydia superphylum (PVC) within the Bacteria, and the Eukarya. We propose that a group of ancient essential protokaryotic signature proteins (PSPs) originating in LUCA were incorporated into ancestors of PVC Bacteria and Eukarya. Tubulins, ubiquitin system enzymes and sterol-synthesizing enzymes are consistent with early origins of these features shared between the PVC superphylum and Eukarya...
June 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28549739/characterization-of-a-major-facilitator-superfamily-transporter-in-shiraia-bambusicola
#20
Huaxiang Deng, Ruijie Gao, Xiangru Liao, Yujie Cai
Reactive oxygen species (ROS) generated by photo-activated hypocrellin from Shiraia bambusicola are detrimental to cellular macromolecules. However, S. bambusicola can still maintain excellent morphology during continuous hypocrellin production, indicating an extraordinary autoresistance system that protects against the harmful ROS. In this study, a major facilitator superfamily transporter (MFS) was isolated from S. bambusicola and deleted using the clustered regularly interspaced short palindromic repeat sequences (CRISPR)/Cas9 system...
May 24, 2017: Research in Microbiology
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