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Research in Microbiology

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https://www.readbyqxmd.com/read/28214542/several-hpept1-transported-drugs-are-substrates-of-the-e-coli-proton-coupled-oligopeptide-transporter-ydgr
#1
Bala K Prabhala, Nanda G Aduri, Mazhar Iqbal, Moazur Rahman, Michael Gajhede, Paul R Hansen, Osman Mirza
Proton-dependent oligopeptide transporters (POTs) are secondary active transporters found in all kingdoms of life. POTs utilize the proton electrochemical gradient for the uptake of nutrient dipeptides and tripeptides. The human POT hPepT1 is known to transport a number of drugs. As part of ongoing studies on substrate specificities of POTs from E. coli, our aim in this study was to investigate whether bacterial POTs could also transport these drugs. For this, we selected the common orally administered drugs sulpiride, bestatin, valacyclovir, ampicillin and oseltamivir, that are all transported by hPepT1...
February 15, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28213104/isolation-and-characterization-of-a-virulent-bacteriophage-infecting-acinetobacter-johnsonii-from-activated-sludge
#2
Niansi Fan, Rong Qi, Min Yang
A double-stranded DNA phage named AJO1, infecting Acinetobacter johnsonii, which plays an important role in wastewater treatment, was isolated from activated sludge in a full-scale municipal wastewater treatment plant. Based on morphological taxonomy, AJO1, with an icosahedral head 55 ± 2 nm in diameter and a non-contractile tail 8 ± 2 nm in length, was classified as a member of the Podoviridae family. Bacterial infection characteristics were as follows: no polyvalent infectivity, optimal multiplicity of infection of 10(-2); eclipse and burst size of 30 min and 51...
February 14, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28161485/identification-and-characterization-of-the-genes-encoding-carbon-monoxide-dehydrogenase-in-terrabacter-carboxydivorans
#3
Jae Ho Lee, Sae Woong Park, Young Min Kim, Jeong-Il Oh
Terrabacter carboxydivorans is able to grow aerobically at low concentrations of carbon monoxide (CO) as a sole source of carbon and energy. The genes for carbon monoxide dehydrogenase (CO-DH) were cloned from T. carboxydivorans and analyzed. The operon encoding T. carboxydivorans CO-DH was composed of three structural genes with the transcriptional order of cutB, cutC and cutA, as well as an additional accessory gene (orf4). Phylogenetic analysis of CutA revealed that T. carboxydivorans CO-DH was classified into a group distinct from previously characterized CO-DHs...
February 1, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28141994/corrigendum-to-the-microbiome-of-a-striped-dolphin-stenella-coeruleoalba-stranded-in-portugal-res-microbiol-2017-85-93
#4
Filipa Godoy-Vitorino, Arnold Rodriguez-Hilario, Ana Luísa Alves, Filipa Gonçalves, Beatriz Cabrera-Colon, Cristina Sousa Mesquita, Pedro Soares-Castro, Marisa Ferreira, Ana Marçalo, José Vingada, Catarina Eira, Pedro Miguel Santos
No abstract text is available yet for this article.
January 28, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28131895/biofilm-formation-and-cellulose-expression-by-bordetella-avium-197n-the-causative-agent-of-bordetellosis-in-birds-and-an-opportunistic-respiratory-pathogen-in-humans
#5
Kimberley McLaughlin, Ayorinde O Folorunso, Yusuf Y Deeni, Dona Foster, Oksana Gorbatiuk, Simona M Hapca, Corinna Immoor, Anna Koza, Ibrahim U Mohammed, Olena Moshynets, Sergii Rogalsky, Kamil Zawadzki, Andrew J Spiers
Although bacterial cellulose synthase (bcs) operons are widespread within the Proteobacteria phylum, subunits required for the partial-acetylation of the polymer appear to be restricted to a few γ-group soil, plant-associated and phytopathogenic pseudomonads, including Pseudomonas fluorescens SBW25 and several Pseudomonas syringae pathovars. However, a bcs operon with acetylation subunits has also been annotated in the unrelated β-group respiratory pathogen, Bordetella avium 197N. Our comparison of subunit protein sequences and GC content analyses confirms the close similarity between the B...
January 25, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28111289/ancient-highly-conserved-proteins-from-a-luca-with-complex-cell-biology-provide-evidence-in-support-of-the-nuclear-compartment-commonality-nucom-hypothesis
#6
REVIEW
James T Staley, John A Fuerst
The nuclear compartment commonality (NuCom) hypothesis posits a complex last common ancestor (LUCA) with membranous compartments including a nuclear membrane. Such a LUCA then evolved to produce two nucleated lineages of the tree of life: the Planctomycetes-Verrucomicrobia-Chlamydia superphylum (PVC) within the Bacteria, and the Eukarya. We propose that a group of ancient essential protokaryotic signature proteins (PSPs) originating in LUCA were incorporated into ancestors of PVC Bacteria and Eukarya. Tubulins, ubiquitin system enzymes and sterol-synthesizing enzymes are consistent with early origins of these features shared between the PVC superphylum and Eukarya...
January 19, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28093321/sulfur-compound-oxidation-and-carbon-co-assimilation-in-the-haloalkaliphilic-sulfur-oxidizers-thioalkalivibrio-versutus-and-thioalkalimicrobium-aerophilum
#7
Wee Kiong Ang, Maisarah Mahbob, Rabeb Dhouib, Ulrike Kappler
Alkaliphilic sulfur-oxidizing bacteria are highly abundant in naturally occurring soda lakes, where they are found both in surface waters and sediments. Here we studied oxidation of sulfide and thiosulfate in batch cultures of Thioalkalimicrobium aerophilum and Thioalkalivibrio versutus, two species that represent different metabolic types, as indicated by the absence or presence of sulfur production during growth, respectively. With thiosulfate, both species showed the expected sulfur oxidation patterns; however, during growth on sulfide, both T...
January 14, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28093322/anti-pathogenic-and-probiotic-attributes-of-lactobacillus-salivarius-and-lactobacillus-plantarum-strains-isolated-from-feces-of-algerian-infants-and-adults
#8
Hamza Ait Seddik, Farida Bendali, Benoit Cudennec, Djamel Drider
Sixty-seven (67) lactic acid bacteria (LAB) isolates belonging to Lactobacillus genus were isolated from human feces and tested for their auto-aggregation and cell surface hydrophobicity in order to establish their adhesion capabilities, a prerequisite for probiotic selection. Strains with the upmost auto-aggregation and cell surface hydrophobicity scores were identified by MALDI-TOF spectrometry and 16S rDNA sequencing as Lactobacillus plantarum (p25lb1 and p98lb1) and Lactobacillus salivarius (p85lb1 and p104lb1)...
January 13, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28040468/characterizations-of-purple-non-sulfur-bacteria-isolated-from-paddy-fields-and-identification-of-strains-with-potential-for-plant-growth-promotion-greenhouse-gas-mitigation-and-heavy-metal-bioremediation
#9
Jakkapan Sakpirom, Duangporn Kantachote, Tomorn Nunkaew, Eakalak Khan
This study was aimed at selecting purple non-sulfur bacteria (PNSB) isolated from various paddy fields, including Cd- and Zn-contaminated paddy fields, based on their biofertilizer properties. Among 235 PNSB isolates, strain TN110 was most effective in plant growth-promoting substance (PGPS) production, releasing 3.2 mg/L of [Formula: see text] , 4.11 mg/L of 5-aminolevulinic acid (ALA) and 3.62 mg/L of indole-3-acetic acid (IAA), and reducing methane emission up to 80%. This strain had nifH, vnfG and anfG, which are the Mo, V and Fe nitrogenase genes encoded for key enzymes in nitrogen fixation under different conditions...
December 28, 2016: Research in Microbiology
https://www.readbyqxmd.com/read/28040467/proteomic-profile-of-susceptible-and-multidrug-resistant-clinical-isolates-of-escherichia-coli-and-klebsiella-pneumoniae-using-label-free-and-immunoproteomic-strategies
#10
Sandra Magalhães, Miguel Aroso, Inês Roxo, Sónia Ferreira, Frederico Cerveira, Elmano Ramalheira, Rita Ferreira, Rui Vitorino
Infectious diseases caused by multidrug-resistant (MDR) Enterobacteriaceae have exponentially increased in the past decade, and are a major concern in hospitals. In the first part of the work, we compared the proteome profile of MDR and susceptible clinical isolates of Escherichia coli and Klebsiella pneumoniae in order to identify possible biological processes associated with drug resistance and susceptible phenotypes, using a label-free approach. In the second part, we used an immunoproteomics approach to identify immunoreactive proteins in the same isolates...
December 28, 2016: Research in Microbiology
https://www.readbyqxmd.com/read/27965152/beneficial-and-detrimental-spore-formers-a-world-of-diversity
#11
EDITORIAL
Véronique Broussolle, Frédéric Carlin, Didier Lereclus, Christina Nielsen-LeRoux, Vincent Sanchis
No abstract text is available yet for this article.
December 10, 2016: Research in Microbiology
https://www.readbyqxmd.com/read/27965151/riptortus-pedestris-and-burkholderia-symbiont-an-ideal-model-system-for-insect-microbe-symbiotic-associations
#12
REVIEW
Kazutaka Takeshita, Yoshitomo Kikuchi
A number of insects establish symbiotic associations with beneficial microorganisms in various manners. The bean bug Riptortus pedestris and allied stink bugs possess an environmentally acquired Burkholderia symbiont in their midgut crypts. Unlike other insect endosymbionts, the Burkholderia symbiont is easily culturable and genetically manipulatable outside the host. In conjunction with the experimental advantages of the host insect, the Riptortus-Burkholderia symbiosis is an ideal model system for elucidating the molecular bases underpinning insect-microbe symbioses, which opens a new window in the research field of insect symbiosis...
December 10, 2016: Research in Microbiology
https://www.readbyqxmd.com/read/27615066/the-microbiome-of-a-striped-dolphin-stenella-coeruleoalba-stranded-in-portugal
#13
Filipa Godoy-Vitorino, Arnold Rodriguez-Hilario, Ana Luísa Alves, Filipa Gonçalves, Beatriz Cabrera-Colon, Cristina Sousa Mesquita, Pedro Soares-Castro, Marisa Ferreira, Ana Marçalo, José Vingada, Catarina Eira, Pedro Miguel Santos
Infectious diseases with epizootic consequences have not been fully studied in marine mammals. Presently, the unprecedented depth of sequencing, made available by high-throughput approaches, allows detailed comparisons of the microbiome in health and disease. This is the first report of the striped dolphin microbiome in different body sites. Samples from one striped female edematous dolphin were acquired from a variety of body niches, including the blowhole, oral cavity, oral mucosa, tongue, stomach, intestines and genital mucosa...
January 2017: Research in Microbiology
https://www.readbyqxmd.com/read/27492955/h-ns-an-overarching-regulator-of-the-vibrio-cholerae-life-cycle
#14
REVIEW
Julio C Ayala, Anisia J Silva, Jorge A Benitez
Vibrio cholerae has become a model organism for studies connecting virulence, pathogen evolution and infectious disease ecology. The coordinate expression of motility, virulence and biofilm enhances its pathogenicity, environmental fitness and fecal-oral transmission. The histone-like nucleoid structuring protein negatively regulates gene expression at multiple phases of the V. cholerae life cycle. Here we discuss: (i) the regulatory and structural implications of H-NS chromatin-binding in the two-chromosome cholera bacterium; (ii) the factors that counteract H-NS repression; and (iii) a model for the regulation of the V...
January 2017: Research in Microbiology
https://www.readbyqxmd.com/read/27884784/phenotypic-and-genomic-characterization-of-the-antimicrobial-producer-rheinheimera-sp-eprs3-isolated-from-the-medicinal-plant-echinacea-purpurea-insights-into-its-biotechnological-relevance
#15
Luana Presta, Emanuele Bosi, Marco Fondi, Isabel Maida, Elena Perrin, Elisangela Miceli, Valentina Maggini, Patrizia Bogani, Fabio Firenzuoli, Vincenzo Di Pilato, Gian Maria Rossolini, Alessio Mengoni, Renato Fani
In recent years, there has been increasing interest in plant microbiota; however, despite medicinal plant relevance, very little is known about their highly complex endophytic communities. In this work, we report on the genomic and phenotypic characterization of the antimicrobial compound producer Rheinheimera sp. EpRS3, a bacterial strain isolated from the rhizospheric soil of the medicinal plant Echinacea purpurea. In particular, EpRS3 is able to inhibit growth of different bacterial pathogens (Bcc, Acinetobacterbaumannii, and Klebsiellapneumoniae) which might be related to the presence of gene clusters involved in the biosynthesis of different types of secondary metabolites...
November 22, 2016: Research in Microbiology
https://www.readbyqxmd.com/read/27884783/secondary-multidrug-efflux-pump-mutants-alter-escherichia-coli-biofilm-growth-in-the-presence-of-cationic-antimicrobial-compounds
#16
Denice C Bay, Carol A Stremick, Carmine J Slipski, Raymond J Turner
Escherichia coli possesses many secondary active multidrug resistance transporters (MDTs) that confer overlapping substrate resistance to a broad range of antimicrobials via proton and/or sodium motive force. It is uncertain whether redundant MDTs uniquely alter cell survival when cultures grow planktonically or as biofilms. In this study, the planktonic and biofilm growth and antimicrobial resistance of 13 E. coli K-12 single MDT gene deletion strains in minimal and rich media were determined. Antimicrobial tolerance to tetracycline, tobramycin and benzalkonium were also compared for each ΔMDT strain...
November 22, 2016: Research in Microbiology
https://www.readbyqxmd.com/read/27884785/viable-but-non-culturable-state-and-toxin-gene-expression-of-enterohemorrhagic-escherichia-coli-o157-under-cryopreservation
#17
Junyan Liu, Rong Zhou, Lin Li, Brian M Peters, Bing Li, Chii-Wann Lin, Tsung-Liang Chuang, Dingqiang Chen, Xihong Zhao, Zhiyong Xiong, Zhenbo Xu, Mark E Shirtliff
As major food-borne pathogens worldwide, Escherichia coli are capable of toxin production directly causing severe human disease. However, routine methods are incapable of detecting viable but non-culturable (VBNC) bacteria in food products and raw materials, leading to false-negative identification. In this study, VBNC E. coli O157 strains were acquired after cryopreservation at -20 °C, with and without freeze-thawing; morphology was observed to be of shorter rod-shape, and toxin expression remained at relatively high levels...
November 21, 2016: Research in Microbiology
https://www.readbyqxmd.com/read/27884782/is-the-plant-associated-microbiota-of-thymus-spp-adapted-to-plant-essential-oil
#18
Alice Checcucci, Isabel Maida, Giovanni Bacci, Cristina Ninno, Anna Rita Bilia, Sauro Biffi, Fabio Firenzuoli, Guido Flamini, Renato Fani, Alessio Mengoni
We examined whether the microbiota of two related aromatic thyme species, Thymus vulgaris and Thymus citriodorus, differs in relation to the composition of the respective essential oil (EO). A total of 576 bacterial isolates were obtained from three districts (leaves, roots and rhizospheric soil). They were taxonomically characterized and inspected for tolerance to the EO from the two thyme species. A district-related taxonomic pattern was found. In particular, high taxonomic diversity among the isolates from leaves was detected...
November 21, 2016: Research in Microbiology
https://www.readbyqxmd.com/read/27845247/indole-3-acetic-acid-production-via-the-indole-3-pyruvate-pathway-by-plant-growth-promoter-rhizobium-tropici-ciat-899-is-strongly-inhibited-by-ammonium
#19
Eddie Luidy Imada, Amanda Alves de Paiva Rolla Dos Santos, André Luiz Martinez de Oliveira, Mariangela Hungria, Elisete Pains Rodrigues
Like many rhizobia, Rhizobium tropici produces indole-3-acetic acid (IAA), an important signal molecule required for root hair infection in rhizobia-legume symbioses. However, the IAA biosynthesis pathway and its regulation by R. tropici are still poorly understood. In this study, IAA synthesis and the effects of mineral N in IAA production by R. tropici CIAT 899 were verified by ultraperformance liquid chromatography-mass spectrometry (UPLC-MS). Furthermore, expression of genes related to IAA biosynthesis and metabolism were evaluated by RT-qPCR...
November 11, 2016: Research in Microbiology
https://www.readbyqxmd.com/read/27818282/isolation-of-bacteriophages-and-their-application-to-control-pseudomonas-aeruginosa-in-planktonic-and-biofilm-models
#20
Magdalena Kwiatek, Sylwia Parasion, Paweł Rutyna, Lidia Mizak, Romuald Gryko, Marcin Niemcewicz, Alina Olender, Małgorzata Łobocka
Pseudomonas aeruginosa is frequently identified as a cause of diverse infections and chronic diseases. It forms biofilms and has natural resistance to several antibiotics. Strains of this pathogen resistant to new-generation beta-lactams have emerged. Due to the difficulties associated with treating chronic P. aeruginosa infections, bacteriophages are amongst the alternative therapeutic options being actively researched. Two obligatorily lytic P. aeruginosa phages, vB_PaeM_MAG1 (MAG1) and vB_PaeP_MAG4 (MAG4), have been isolated and characterized...
November 4, 2016: Research in Microbiology
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