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Research in Microbiology

Klaas Martinus Pos
No abstract text is available yet for this article.
September 14, 2018: Research in Microbiology
Alexandro Guterres, Jonathan Gonçalves, Elba Regina Sampaio de Lemos
Bartonellosis is an emerging serious diseases of zoonotic relevance caused by an expanding number of recently discovered species of Bartonella. Many studies use partial gltA gene sequencing as the method of choice for species attribution, and a plethora of studies have utilized only this gene to describe Bartonella diversity. We observe a lack of correspondence between the phylogenies constructed using the complete gltA gene sequences and the small gene fragment usually used in phylogenetic analyzes. Everything indicates that important changes occurred by using larger fragments...
September 11, 2018: Research in Microbiology
Anke Licht, Martin Bommer, Tobias Werther, Kristin Neumann, Carolin Hobe, Erwin Schneider
Canonical ATP-binding cassette import systems rely on extracellular substrate binding proteins (SBP) for function. In gram-negative bacteria, SBPs are usually freely diffusible in the periplasm and, where studied, exist in excess over their cognate transporters. However, in vitro studies with the maltose transporter of Escherichia coli (MalFGK2 ) have demonstrated that mechanistically one copy of its SBP (MalE) per transport complex is sufficient for activity. To address whether such a condition is physiologically relevant, we have characterized a homolog of the E...
September 4, 2018: Research in Microbiology
Zhongxin Luo, Shengjie Li, Kun Hou, Guodong Ji
In this study, the spatial and seasonal bacterioplankton community dynamics were investigated in the main channel of the Middle Route of the South-to-North Water Diversion Project (MRP) using Illumina HiSeq sequencing. Water samples were collected in spring and summer from south to north at eight water quality monitoring stations, respectively. The results showed that seasonal changes had a more pronounced effect on the bacterioplankton community compositions (BCCs) than spatial variation. The diversity analysis results indicated that samples of summer have more operational taxonomic units (OTUs), higher richness and diversity than those in spring...
September 1, 2018: Research in Microbiology
Paulina Aguirre, Karlo Guerrero, Aminael Sanchez-Rodriguez, Juan Carlos Gentina, Axel Schippers
The purpose of this study was to compare the efficacy of galactose and high initial ferrous iron concentrations as inducers for extracellular polymeric substances (EPS) production in planktonic cells of Leptospirillum ferrooxidans and to study cell attachment to a mineral surface in comparison to cells not exposed to such substances. L. ferrooxidans was successfully adapted to grow in a modified 9K medium at different concentrations of galactose (0.15, 0.25, 0.35%) and also at different initial ferrous iron concentrations (18, 27, 36 g/L), which are higher than 9K medium (9 g/L)...
September 1, 2018: Research in Microbiology
Li-Zhu Liu, Zhen-Yuan Nie, Yi Yang, Xuan Pan, Xu Xia, Yu-Hang Zhou, Jin-Lan Xia, Li-Juan Zhang, Xiang-Jun Zhen, Hong-Ying Yang
For the first time, synchrotron radiation (SR) -based carbon K-edge X-ray absorption near edge structure (XANES) spectroscopy in-situ characterization was conducted to evaluate the evolution of superficial (about 10 nm) organic components of extracellular polymeric substances (EPS) of thermoacidophilic archaeon Acidianus manzaensis YN-25 acclimated with different energy substrates (FeS2 , CuFeS2 , S0 , FeSO4 ). The atomic force microscopy (AFM) morphology scanning showed that the strain acclimated with different energy substrates varied a lot in EPS amount...
September 1, 2018: Research in Microbiology
Aurélie Roth, Cedric Govaerts
The secondary transporter LmrP from Lactoccoccuslactis is a remarkable model to study the molecular basis of secondary multidrug transport. This review article addresses more than twenty years of research about transport activity, substrates range, conformational dynamics and mechanistic models of drug export for LmrP. Several studies have shown that the transporter alternates between inward-open and outward-open conformations and that the transition is regulated by the protonation state of key acidic residues and is further modulated by the lipid environment...
August 23, 2018: Research in Microbiology
Francisco Issotta, Ana Moya-Beltrán, Cristóbal Mena, Paulo C Covarrubias, Christian Thyssen, Sören Bellenberg, Wolfgang Sand, Raquel Quatrini, Mario Vera
The family Acidiferrobacteraceae (order Acidiferrobacterales) currently contains Gram negative, neutrophilic sulfur oxidizers such as Sulfuricaulis and Sulfurifustis, as well as acidophilic iron and sulfur oxidizers belonging to the Acidiferrobacter genus. The diversity and taxonomy of the genus Acidiferrobacter has remained poorly explored. Although several metagenome and bioleaching studies have identified its presence worldwide, only two strains, namely Acidiferrobacter thiooxydans DSM 2932T , and Acidiferrobacter spp...
August 22, 2018: Research in Microbiology
Paulo C Covarrubias, Ana Moya-Beltrán, Joaquin Atavales, Francisco Moya-Flores, Pablo S Tapia, Lillian G Acuña, Silvia Spinelli, Raquel Quatrini
General knowledge on the diversity and biology of microbial viruses infecting bacterial hosts from extreme acidic environments lags behind most other econiches. In this study, we analyse the AcaML1 virus occurrence in the taxon, its genetic composition and infective behaviour under standard acidic and SOS-inducing conditions to assess its integrity and functionality. Occurrence analysis in sequenced acidithiobacilli showed that AcaML1-like proviruses are confined to the mesothermophiles Acidithiobacillus caldus and Thermithiobacillus tepidarius...
August 20, 2018: Research in Microbiology
Ringo Schwabe, Marlene Kirstin Anke, Katarzyna Szymańska, Oliver Wiche, Dirk Tischler
The Actinobacterium Gordonia rubripertincta CWB2 (DSM 46758) produces hydroxamate-type siderophores (188 mg L-1 ) under iron limitation. Analytical reversed-phase HPLC allowed determining a single peak of ferric iron chelating compounds from culture broth which was confirmed by the Fe-CAS assay. Elution profile and its absorbance spectrum were similar to those of commercial (des)ferrioxamine B which was used as reference compound. This confirms previously made assumptions and shows for the first time that the genus Gordonia produces desferrioxamine-like siderophores...
August 20, 2018: Research in Microbiology
Hin-Chung Wong, Tzu-Yun Wang, Chu-Wen Yang, Chung-Tao Tang, Chingwen Ying, Chun-Hsiung Wang, Wei-Hau Chang
Vibrio parahaemolyticus is a human enteropathogenic bacterium and is also pathogenic to shrimp and finfish. In a search for a biocontrol agent for V. parahaemolyticus and other pathogenic Vibrio species, a lytic phage VP06 was isolated from oyster using V. parahaemolyticus as the host. VP06 is a Siphoviridae phage with a polyhedral head and a long tail. The genome sequence of VP06 was 75,893 nucleotides in length and the G + C content was 49%; a total of 101 CDSs were identified in VP06, of which 39 exhibited functional domains/motifs...
August 2, 2018: Research in Microbiology
Himel N Khaleque, Raihan Shafique, Anna H Kaksonen, Naomi J Boxall, Elizabeth L J Watkin
In this study, the differential protein expression of the acidophilic halophile, Acidihalobacter prosperus DSM 14174 (strain V6) was studied with the aim of understanding its mechanisms of tolerance to high chloride ion stress in the presence of low pH, using Sequential Window Acquisition of all Theoretical Mass Spectra (SWATH-MS). In acidophiles, chloride stress results in both osmotic stress as well as acidification of the cytoplasm due to the ability of chloride to permeate the cell membrane and disrupt the reversed transmembrane potential which normally extrudes protons...
July 19, 2018: Research in Microbiology
Carmen Falagán, D Barrie Johnson
The ability of acidophilic bacteria to grow in the presence of elevated concentrations of cationic transition metals, though varying between species, has long been recognized to be far greater than that of most neutrophiles. Conversely, their sensitivity to both inorganic and organic anions, with the notable exception of sulfate, has generally been considered to be far more pronounced. We have compared the tolerance of different species of mineral-oxidizing Acidithiobacillus and Sulfobacillus, and the heterotrophic iron-reducer Acidiphilium cryptum, to copper and chloride when grown on ferrous iron, hydrogen or glucose as electron donors at pH values between 2...
July 19, 2018: Research in Microbiology
Fernanda Rodrigues-Costa, Rodrigo Cayô, Adriana Pereira Matos, Raquel Girardello, Willames M B S Martins, Floristher Elaine Carrara-Marroni, Ana Cristina Gales
Nine carbapenem-resistant Acinetobacter baumannii isolates carrying blaOXA-231 and an ISAba1 upstream occAB1 were evaluated. They were clonally related and belonged to ST107. An OXA-143-producing A. baumannii ST107 strain (Ac-148) that did not possess ISAba1 upstream occAB1 was included in the analysis. Reduction in the expression of occAB1 and a 4-fold increase of carbapenem MICs were observed for all isolates, except for the Ac-148 strain, probably due to the presence of ISAba1 upstream occAB1 but in the same transcriptional orientation...
July 9, 2018: Research in Microbiology
Catherine Rouzeau, Adilya Dagkesamanskaya, Krzysztof Langer, Jérôme Bibette, Jean Baudry, Denis Pompon, Véronique Anton-Leberre
Adjustment of plasmid copy number resulting from the balance between positive and negative impacts of borne synthetic genes, plays a critical role in the global efficiency of multistep metabolic engineering. Differential expression of co-expressed engineered genes is frequently observed depending on growth phases, metabolic status and triggered adjustments of plasmid copy numbers, constituting a dynamic process contributing to minimize global engineering burden. A yeast model involving plasmid based expression of phosphoribulokinase (PRKp), a key enzyme for the reconstruction of synthetic Calvin cycle, was designed to gain further insights into such a mechanism...
July 2018: Research in Microbiology
Adriana B Cesari, Natalia S Paulucci, María A Biasutti, Gustavo M Morales, Marta S Dardanelli
We evaluate the behavior of the membrane of Bradyrhizobium sp. SEMIA6144 during adaptation to polyethylene glycol (PEG). A dehydrating effect on the morphology of the cell surface, as well as a fluidizing effect on the membrane was observed 10 min after PEG shock; however, the bacteria were able to restore optimal membrane fluidity. Shock for 1 h caused an increase of lysophosphatidylethanolamine in the outer membrane at the expense of phosphatidylcholine and phosphatidylethanolamine (PE), through an increase in phospholipase activity...
July 2018: Research in Microbiology
Carolina E Cabezas, Alan C Briones, Camila Aguirre, Coral Pardo-Esté, Juan Castro-Severyn, César R Salinas, María S Baquedano, Alejandro A Hidalgo, Juan A Fuentes, Eduardo H Morales, Claudio A Meneses, Eduardo Castro-Nallar, Claudia Paz Saavedra
Salmonella Typhimurium is an intracellular pathogen that is capable of generating systemic fever in a murine model. Over the course of the infection, Salmonella faces different kinds of stressors, including harmful reactive oxygen species (ROS). Various defence mechanisms enable Salmonella to successfully complete the infective process in the presence of such stressors. The transcriptional factor SlyA is involved in the oxidative stress response and invasion of murine macrophages. We evaluated the role of SlyA in response to H2 O2 and NaOCl and found an increase of slyA expression upon exposure to these toxics...
July 2018: Research in Microbiology
Alma Laura Díaz-Pérez, Cinthia Núñez, Victor Meza Carmen, Jesús Campos-García
Pseudomonas aeruginosa metabolizes leucine through the leucine/isovalerate utilization pathway, whose enzymes are encoded in the liuRABCDE gene cluster (liu). In this study, we investigated the role of the LiuR protein in the liu cluster regulation. Our results indicated that liu expression is regulated at the transcriptional level by LiuR. Mobility shift assays using purified recombinant His-tagged LiuR showed that it was able to bind at the promoter region of liuR, in a dose-dependent manner. Results revealed that expression of the liu operon is subjected to carbon catabolite repression control (CCR); protein LiuD was strongly expressed in the presence of leucine, but it was repressed in the presence of glucose or succinate...
July 2018: Research in Microbiology
Lea Wittorf, Christopher M Jones, Germán Bonilla-Rosso, Sara Hallin
Reduction of nitrite to nitric oxide in denitrification is catalysed by two different nitrite reductases, encoded by nirS or nirK. Long considered mutually exclusive and functionally redundant in denitrifying bacteria, we show expression of both genes co-occurring in Pseudomonas stutzeri. The differential expression patterns between strain AN10 and JM300 in relation to oxygen and nitrate and their different denitrification phenotypes, with AN10 reducing nitrate more rapidly and accumulating nitrite, suggest that nirS and nirK can have different roles...
July 2018: Research in Microbiology
Xin Zhao, Rui Liu, Hao Tang, George Osei-Adjei, Shungao Xu, Ying Zhang, Xinxiang Huang
Bacterial non-coding RNAs (ncRNAs) are widely studied and found to play important roles in regulating various cellular processes. Recently, many ncRNAs have been discovered to be transcribed or processed from 3' untranslated regions (3' UTRs). Here we reported a novel 3' UTR-derived ncRNA, RibS, which could influence biofilm formation of Salmonella enterica serovar Typhi (S. Typhi). RibS was confirmed to be a ∼700 nt processed product produced by RNase III-catalyzed cleavage from the 3' UTR of riboflavin synthase subunit alpha mRNA, RibE...
July 2018: Research in Microbiology
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