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Reproduction, Fertility, and Development

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https://www.readbyqxmd.com/read/28641706/delayed-onset-of-puberty-in-male-offspring-from-bisphenol-a-treated-dams-is-followed-by-the-modulation-of-gene-expression-in-the-hypothalamic-pituitary-testis-axis-in-adulthood
#1
Isabela M Oliveira, Renata M Romano, Patricia de Campos, Monica D Cavallin, Claudio A Oliveira, Marco A Romano
Bisphenol A (BPA) is a synthetic endocrine-disrupting chemical of high prevalence in the environment, which may affect the function of the hypothalamic-pituitary-testis (HPT) axis in adult rats. The aim of the present study was to evaluate whether exposure to BPA during hypothalamic sexual differentiation at doses below the reproductive no observable adverse effect level of the World Health Organization causes changes in the regulation of the HPT axis. For this, 0.5 or 5mgkg-1 BPA was injected subcutaneously to the mothers from gestational day 18 to postnatal day (PND) 5...
June 23, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28625274/beneficial-effects-of-melatonin-on-in-vitro-embryo-production-from-juvenile-goat-oocytes
#2
Sandra Soto-Heras, Montserrat Roura, Maria G Catalá, Irene Menéndez-Blanco, Dolors Izquierdo, Ali A Fouladi-Nashta, Maria T Paramio
Melatonin is a universal antioxidant that improves in vitro embryo production in several species. The aims of this study were to determine the melatonin concentration in the ovarian follicular fluid (FF) of juvenile goats and the effect of melatonin during in vitro maturation (IVM) on embryo development. The FF melatonin concentration was 0.57--1.07×10-9 M, increasing with follicular diameter. Oocytes were matured, fertilised and cultured under conventional conditions. Blastocyst development, embryo quality and levels of reactive oxygen species (ROS) and reduced glutathione were assessed...
June 19, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28610652/novel-insights-into-the-role-of-cell-free-seminal-mrnas-on-semen-quality-and-cryotolerance-of-spermatozoa-in-bulls-bos-taurus
#3
Munivenkatappa Shilpa, Sellappan Selvaraju, Venkataswamy GirishKumar, Sivashanmugam Parthipan, Krishnan B Binsila, Arunachalam Arangasamy, Janivara Parameshwaraiah Ravindra
The aim of the present study was to ascertain the effectiveness of seminal plasma mRNAs as markers to assess the reproductive performance of bulls. Semen samples (33 ejaculates) from 11 bulls were evaluated for sperm kinematic and functional parameters. Total RNA was isolated from cell-free seminal (cfs) using TRIzol LS reagent and the concentration of cfs-RNA was 24.4±2.3µgmL-1 seminal plasma. The cfs-RNA was fragmented to a size of 25-500bp. Of the cfs-mRNAs screened using real time PCR, expression of protamine 1 (PRM1) was positively (P<0...
June 14, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28610651/increased-high-molecular-weight-adiponectin-but-decreased-total-adiponectin-and-kisspeptin-in-central-precocious-puberty-compared-with-aged-matched-prepubertal-girls
#4
Chantacha Sitticharoon, Maynart Sukharomana, Supawadee Likitmaskul, Malika Churintaraphan, Pailin Maikaew
The aim of the present study was to compare serum leptin, kisspeptin, total adiponectin, high molecular weight (HMW) adiponectin and neuropeptide Y (NPY) levels between girls with central precocious puberty (CPP; n=26, 7-9.5 years old) and age-matched controls (n=29) including or excluding obese girls. Leptin and NPY levels were comparable between CPP and control girls. Kisspeptin levels were lower in the CPP than control group, and were positively correlated with oestrogen in the control group and with systolic and diastolic blood pressure in the CPP group...
June 14, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28595033/temperature-gradients-in-vivo-influence-maturing-male-and-female-gametes-in-mammals-evidence-from-the-cow
#5
R H F Hunter, F López-Gatius, O López-Albors
Since 1980 several reports have indicated that temperatures vary between preovulatory follicles and other ovarian tissues in rabbit, cow, pig and human. However, these observations did not achieve prominence; they were regarded as artefacts due to the use of anaesthetics and open surgery (laparotomy). Recently, without resorting to anaesthesia or surgery, direct measurements of temperature in preovulatory follicles have been performed in the cow by means of a thermistor probe introduced into the antrum under ultrasonic guidance...
June 9, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28570851/effect-of-seminal-plasma-from-high-and-low-fertility-bulls-on-cauda-epididymal-sperm-function
#6
S A Holden, B Fernandez-Fuertes, E M Murphy, P Lonergan, S Fair
The aim of this study was to characterise the effect of seminal plasma (SP) from bulls of high or low fertility on sperm function. First, the effect of SP on the motility of fresh cauda epididymal spermatozoa (CES) and frozen-thawed ejaculated spermatozoa was assessed (Experiment 1a). Seminal plasma was then collected from bulls of known high and low fertility. Pooled CES were incubated in the SP from each bull, diluted and assessed for motility and viability on Days 1, 2, 3 and 5 after packaging as fresh semen (Experiment 1b)...
June 2, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28566114/effects-of-ovarian-disaggregation-on-adult-murine-follicle-yield-and-viability
#7
Fiona Young, John Drummond, Emma Akers, Louise Bartle, David Kennedy, Mohammad Asaduzzaman
Follicles are isolated from ovaries for numerous reasons, including IVM, but adult murine yields are <2 folliclesmg-1. The aim of the present study was to optimise ovarian disaggregation and develop methods applicable to the rapid screening of follicle viability. Ovaries from adult mice (n=7) were halved and disaggregated mechanically, or by using collagenase IV (Col-IV; 590UmL-1) or animal origin-free collagenase IV (AOF) at 590 or 1180UmL-1. Isolated follicles were stained with 4',6'-diamidino-2-phenylindole (DAPI; nuclei), chloromethyl-X-rosamine (CMXRos; mitochondria) or fluorescein isothiocyanate-conjugated anti-α-tubulin antibody...
June 1, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28553998/splicing-related-single-nucleotide-polymorphism-of-rab-member-of-ras-oncogene-family-like-2b-rabl2b-jeopardises-semen-quality-in-chinese-holstein-bulls
#8
Xiuge Wang, Xiaohui Cui, Yan Zhang, Haisheng Hao, Zhihua Ju, Deyu Liu, Qiang Jiang, Chunhong Yang, Yan Sun, Changfa Wang, Jinming Huang, Huabin Zhu
RAB, member of RAS oncogene family like 2B (RABL2B) is a member of a poorly characterised clade of the RAS GTPase superfamily, which plays an essential role in male fertility, sperm intraflagellar transport and tail assembly. In the present study, we identified a novel RABL2B splice variant in bovine testis and spermatozoa. This splice variant, designated RABL2B-TV, is characterised by exon 2 skipping. Moreover, a single nucleotide polymorphism (SNP), namely c.125G>A, was found within the exonic splicing enhancer (ESE) motif, indicating that the SNP caused the production of the RABL2B-TV aberrant splice variant...
May 30, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28531375/mouse-minipuberty-coincides-with-gonocyte-transformation-into-spermatogonial-stem-cells-a-model-for-human-minipuberty
#9
Ruili Li, Amanda Vannitamby, Sarah S K Yue, David Handelsman, John Hutson
As the transient postnatal hormone surge in humans, known as 'minipuberty', occurs simultaneously with key steps in germ-cell development, we investigated whether similar changes occur in the hypothalamic-pituitary-testicular axis of neonatal mice at a time that would coincide with gonocyte transformation into spermatogonial stem cells (SSC). Serum and testes were collected from C57Bl/6 mice at embryonic Day 17 (E17), birth (postnatal Day 0; P0) and daily until P10. Serum FSH and testosterone levels in both serum and testes were analysed and gene expression of FSH receptor (Fshr), luteinising hormone receptor (Lhr), anti-Müllerian hormone (Amh), octamer-binding transcription factor 4 (Oct-4), membrane type 1 metalloprotease (Mt1-mmp), proto-oncogene C-kit and promyelocytic leukaemia zinc finger (Plzf ) was quantified by real-time polymerase chain reaction...
May 23, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28521851/regulation-of-interleukin-11-expression-in-ovulatory-follicles-of-the-rat-ovary
#10
You-Jee Jang, You-Jee Jang, Jae-Il Park, Jae-Il Park, Seong-Eun Jeong, Seong-Eun Jeong, You-Mi Seo, You-Mi Seo, Phuong T M Dam, Phuong T M Dam, Young-Woo Seo, Young-Woo Seo, Bum-Chae Choi, Bum-Chae Choi, Sang-Jin Song, Sang-Jin Song, Sang-Young Chun, Sang-Young Chun, Moon-Kyoung Cho, Moon-Kyoung Cho
The aim of the present study was to examine the regulation of interleukin (IL)-11 expression, as well as the role of IL-11, during ovulation in gonadotropin-primed immature rats. Injection of equine chorionic gonadotropin (eCG), followed by human CG (hCG) to induce superovulation stimulated expression of the Il11 gene in theca cells within 6h, as revealed by northern blot and in situ hybridisation analyses. Real-time reverse transcription-polymerase chain reaction analysis showed that the IL-11 receptor, α subunit gene was expressed in granulosa and theca cells and that injection of hCG had no effect on its expression...
May 19, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28521850/differential-gene-expression-in-porcine-oviduct-during-the-oestrous-cycle
#11
O S Acuña, M Avilés, R López-Úbeda, A Guillén-Martínez, C Soriano-Úbeda, A Torrecillas, P Coy, M J Izquierdo-Rico
The oviduct undergoes changes under the influence of steroid hormones during the oestrous cycle. However, the molecular mechanisms underlying oviductal regulation are not fully understood. The aim of the present study was to identify the gene expression profile of the porcine oviduct in different stages of the cycle using microarray technology. A systematic study was performed on animals at four different stage: prepubertal gilts, and sows in the preovulatory, postovulatory and luteal phase of the oestrous cycle...
May 19, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28502309/effects-of-vitrification-of-cumulus-enclosed-porcine-oocytes-at-the-germinal-vesicle-stage-on-cumulus-expansion-nuclear-progression-and-cytoplasmic-maturation
#12
Ruth Appeltant, Tamás Somfai, Elisa C S Santos, Thanh Quang Dang-Nguyen, Takashi Nagai, Kazuhiro Kikuchi
Although offspring have been produced from porcine oocytes vitrified at the germinal vesicle (GV) stage, the rate of embryo development remains low. In the present study, nuclear morphology and progression, cumulus expansion, transzonal projections (TZPs), ATP and glutathione (GSH) levels were compared between vitrified cumulus-oocyte complexes (COCs) and control COCs (no cryoprotectant treatment and no cooling), as well as a toxicity control (no cooling). Vitrification was performed with 17.5% (v/v) ethylene glycol and 17...
May 15, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28448789/development-of-pre-implantation-porcine-blastocysts-cultured-within-alginate-hydrogel-systems-either-supplemented-with-secreted-phosphoprotein-1-or-conjugated-with-arg-gly-asp-peptide
#13
Taylor D Laughlin, Jeremy R Miles, Elane C Wright-Johnson, Lea A Rempel, Clay A Lents, Angela K Pannier
Although deficiencies in porcine blastocyst elongation play a significant role in early embryonic mortality and establishment of within-litter developmental variation, the exact mechanisms of elongation are poorly understood. Secreted phosphoprotein 1 (SPP1) is increased within the uterine milieu during early porcine pregnancy and contains an Arg-Gly-Asp (RGD) peptide sequence that binds to cell surface integrins on the uterine endometrium and trophectoderm, promoting cell adhesion and migration. The aim of the present study was to evaluate the development of preimplantation porcine blastocysts encapsulated and cultured within alginate hydrogels either supplemented with SPP1 or conjugated with RGD...
April 28, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28442026/neuromodulatory-effect-of-oestradiol-in-the-metabolism-of-ovarian-progesterone-and-oestradiol-during-dioestrus-ii-participation-of-the-superior-mesenteric-ganglion
#14
Adriana Vega Orozco, Cynthia Bronzi, Sandra Vallcaneras, Zulema Sosa, Marilina Casais
The aims of the present study were to determine: (1) whether oestradiol (E2) in the superior mesenteric ganglion (SMG) modifies the release of ovarian progesterone (P4), androstenedione (A2) and E2, the activity and gene expression of 3β-hydroxysteroid dehydrogenase (3β-HSD) and 20α-HSD and the expression of P450 aromatase (Cyp19a1) and (2) whether any such modifications are related to changes in ovarian nitric oxide (NO) and noradrenaline (NA) levels during dioestrus II. Using an ex vivo SMG-ovarian nervous plexus-ovary system, ovarian P4 release was measured following the addition E2 plus tamoxifen (Txf) (10-6M) to the ganglion, whereas A2, E2, NA and NO were measured following the addition of E2 alone...
April 26, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28442025/protective-effects-of-polydatin-on-experimental-testicular-torsion-and-detorsion-injury-in-rats
#15
Huilian Qiao, He Ma, Wanjun Cao, Hao Chen, Jinhua Wei, Zhen Li
Oxidative stress plays a critical role in the process of testicular torsion and detorsion (T/D). The purpose of the present study was to investigate the protective effect of polydatin (PD) on testicular T/D injury. Rats were randomly divided into three groups, a sham group, a group subjected to 2h torsion followed by 24h detorsion and a group subjected to T/D and injected i.p. with 20mgkg-1 PD 30min before detorsion. Unilateral orchiectomy was performed after 24h of reperfusion. Half the testes were prepared for histological examination by haematoxylin-eosin staining and the terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling (TUNEL) technique...
April 26, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28442024/intracytoplasmic-oxidative-stress-reverses-epigenetic-modifications-in-polycystic-ovary-syndrome
#16
Fatemeh Eini, Marefat Ghaffari Novin, Khojasteh Joharchi, Ahmad Hosseini, Hamid Nazarian, Abbas Piryaei, Arash Bidadkosh
In polycystic ovary syndrome (PCOS), substantial genetic and environmental alterations, along with hyperandrogenism, affect the quality of oocytes and decrease ovulation rates. To determine the mechanisms underlying these alterations caused specifically by an increase in plasma androgens, the present study was performed in experimentally-induced PCOS mice. As the study model, female B6D2F1 mice were treated with dehydroepiandrosterone (DHEA, 6mg per 100g bodyweight). After 20 days, oocytes at the germinal vesicle and metaphase II stages were retrieved from isolated ovaries and subsequent analyses of oocyte quality were performed for each mouse...
April 26, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28420480/comparative-analysis-of-granulosa-cell-gene-expression-in-association-with-oocyte-competence-in-fsh-stimulated-holstein-cows
#17
David A Landry, Chloé Fortin, Anne-Marie Bellefleur, Rémi Labrecque, François-Xavier Grand, Christian Vigneault, Patrick Blondin, Marc-André Sirard
Ovarian stimulation with exogenous FSH followed by FSH withdrawal or 'coasting' is an effective means of increasing the number of oocytes obtainable for the in vitro production of cattle embryos. However, the quality of the oocytes thus obtained varies considerably from one cow to the next. The aim of the present study was to gain a better understanding of the follicular conditions associated with low oocyte developmental competence. Granulosa cells from 94 Holstein cows in a commercial embryo production facility were collected following ovarian stimulation and coasting...
April 19, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28420479/andrographolide-disrupts-meiotic-maturation-by-blocking-cytoskeletal-reorganisation-and-decreases-the-fertilisation-potential-of-mouse-oocytes
#18
Hong-Xing Liang, Sheng-Sheng Lu, Zheng Yan, Yan-Ping Kuang, Xiang-Xing Zhu, Zhi-Guang Yan, Tong Du, Wei-Ran Chai, Hui Long, Qi-Feng Lyu
Andrographolide (AG) is a diterpenoid lactone isolated from the stem and leaves of Andrographis paniculata Nees that is used for the effective treatment of infectious diseases in Asian countries. Previous studies have reported adverse effects of AG on female fertility in rodents; however, the underlying mechanisms are unknown. The aim of the present study was to investigate the effects of AG on the IVM of mouse oocytes and their fertilisation potential. Immature oocytes incubated for 6, 14 or 24h in medium containing 5, 10 or 20μM AG showed time- and dose-dependent decreases in maturation rates compared with the control group...
April 19, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28416050/proteomic-analysis-of-the-endometrium-during-early-pregnancy-in-the-domestic-pig
#19
Justyna Kolakowska, Serhiy Souchelnytskyi, Ravi Kanth Rao Saini, Anita Franczak
Reproductive processes in domestic pigs have been studied extensively. Pigs are one of the main sources of meat for human consumption and are an established model for investigations into mammalian, including human, reproductive physiology. Studies of the uterus during early pregnancy will lead to a better understanding of mechanisms governing pregnancy. Proteomics provides the possibility to explore endometrial functions in an unbiased way. The aim of the study was to compare endometrium harvested from Days 12-13 and 15-16 of pregnancy with the corresponding days of the oestrous cycle...
April 18, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28403915/the-egg-coat-zona-pellucida-3-glycoprotein-evolution-of-its-putative-sperm-binding-region-in-old-world-murine-rodents-rodentia-muridae
#20
Christine A Swann, Steven J B Cooper, William G Breed
In eutherian mammals, before fertilisation can occur the spermatozoon has to bind to, and penetrate, the egg coat, the zona pellucida (ZP). In the laboratory mouse there is good evidence that the primary sperm-binding site is a protein region encoded by Exon 7 of the ZP3 gene and it has been proposed that binding is species specific and evolves by sexual selection. In the present study we investigate these hypotheses by comparing Exon 6 and 7 sequences of ZP3 in 28 species of murine rodents of eight different divisions from Asia, Africa and Australasia, in which a diverse array of sperm morphologies occurs...
April 13, 2017: Reproduction, Fertility, and Development
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