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Reproduction, Fertility, and Development

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https://www.readbyqxmd.com/read/28420480/comparative-analysis-of-granulosa-cell-gene-expression-in-association-with-oocyte-competence-in-fsh-stimulated-holstein-cows
#1
David A Landry, Chloé Fortin, Anne-Marie Bellefleur, Rémi Labrecque, François-Xavier Grand, Christian Vigneault, Patrick Blondin, Marc-André Sirard
Ovarian stimulation with exogenous FSH followed by FSH withdrawal or 'coasting' is an effective means of increasing the number of oocytes obtainable for the in vitro production of cattle embryos. However, the quality of the oocytes thus obtained varies considerably from one cow to the next. The aim of the present study was to gain a better understanding of the follicular conditions associated with low oocyte developmental competence. Granulosa cells from 94 Holstein cows in a commercial embryo production facility were collected following ovarian stimulation and coasting...
April 19, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28420479/andrographolide-disrupts-meiotic-maturation-by-blocking-cytoskeletal-reorganisation-and-decreases-the-fertilisation-potential-of-mouse-oocytes
#2
Hong-Xing Liang, Sheng-Sheng Lu, Zheng Yan, Yan-Ping Kuang, Xiang-Xing Zhu, Zhi-Guang Yan, Tong Du, Wei-Ran Chai, Hui Long, Qi-Feng Lyu
Andrographolide (AG) is a diterpenoid lactone isolated from the stem and leaves of Andrographis paniculata Nees that is used for the effective treatment of infectious diseases in Asian countries. Previous studies have reported adverse effects of AG on female fertility in rodents; however, the underlying mechanisms are unknown. The aim of the present study was to investigate the effects of AG on the IVM of mouse oocytes and their fertilisation potential. Immature oocytes incubated for 6, 14 or 24h in medium containing 5, 10 or 20μM AG showed time- and dose-dependent decreases in maturation rates compared with the control group...
April 19, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28416050/proteomic-analysis-of-the-endometrium-during-early-pregnancy-in-the-domestic-pig
#3
Justyna Kolakowska, Serhiy Souchelnytskyi, Ravi Kanth Rao Saini, Anita Franczak
Reproductive processes in domestic pigs have been studied extensively. Pigs are one of the main sources of meat for human consumption and are an established model for investigations into mammalian, including human, reproductive physiology. Studies of the uterus during early pregnancy will lead to a better understanding of mechanisms governing pregnancy. Proteomics provides the possibility to explore endometrial functions in an unbiased way. The aim of the study was to compare endometrium harvested from Days 12-13 and 15-16 of pregnancy with the corresponding days of the oestrous cycle...
April 18, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28403915/the-egg-coat-zona-pellucida-3-glycoprotein-evolution-of-its-putative-sperm-binding-region-in-old-world-murine-rodents-rodentia-muridae
#4
Christine A Swann, Steven J B Cooper, William G Breed
In eutherian mammals, before fertilisation can occur the spermatozoon has to bind to, and penetrate, the egg coat, the zona pellucida (ZP). In the laboratory mouse there is good evidence that the primary sperm-binding site is a protein region encoded by Exon 7 of the ZP3 gene and it has been proposed that binding is species specific and evolves by sexual selection. In the present study we investigate these hypotheses by comparing Exon 6 and 7 sequences of ZP3 in 28 species of murine rodents of eight different divisions from Asia, Africa and Australasia, in which a diverse array of sperm morphologies occurs...
April 13, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28399989/endo-sirna-deficiency-results-in-oocyte-maturation-failure-and-apoptosis-in-porcine-oocytes
#5
Wanxin Liu, Qi Zhao, Shanhua Piao, Chunsheng Wang, Qingran Kong, Tiezhu An
Both microRNAs (miRNAs) and endogenous small interfering RNAs (endo-siRNAs) play key regulatory roles in gene expression. Some studies have demonstrated that the function of miRNA is suppressed in mouse oocytes, suggesting that endo-siRNA, not miRNA, is essential for female meiosis. This finding has yet to be confirmed in other species. In this study, by knockdown of DICER1, DROSHA and its cofactor DiGeorge syndrome critical region 8 (DGCR8) in porcine oocytes, we found that the proportion of oocytes with DICER1 deficiency that developed to meiosis II (MII) stage was significantly lower than oocytes with DROSHA and DGCR8 deficiency (39...
April 12, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28390473/differential-effects-of-high-and-low-glucose-concentrations-during-lipolysis-like-conditions-on-bovine-in-vitro-oocyte-quality-metabolism-and-subsequent-embryo-development
#6
J De Bie, W F A Marei, V Maillo, L Jordaens, A Gutierrez-Adan, P E J Bols, J L M R Leroy
Lipolytic metabolic conditions are traditionally associated with elevated non-esterified fatty acid (NEFA) concentrations, but may also be accompanied by hyperglycaemia in obesity or by hypoglycaemia during a negative energy balance status. Elevated NEFA concentrations disrupt oocyte and embryo development and quality, but little is known about whether the effects of lipolytic conditions on oocyte developmental competence are modulated by glucose availability. To answer this, bovine cumulus-oocyte complexes (COCs) were matured under different conditions: physiological NEFA (72µM) and normal glucose (5...
April 10, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28390472/pannexin-channels-increase-propidium-iodide-permeability-in-frozen-thawed-dog-spermatozoa
#7
J L Torres, J Palomino, R D Moreno, M De Los Reyes
Pannexins (Panx) are proteins that form functional single membrane channels, but they have not yet been described in dogs. The aim of the present study was to detect Panx1, Panx2 and Panx3 in frozen-thawed dog spermatozoa using flow cytometry and immunofluorescence analyses, evaluating the relationship of these proteins with propidium iodide (PI) in frozen-thawed spermatozoa. Fresh and frozen-thawed dog spermatozoa from eight dogs were preincubated with 3μM PI with or without 15μM carbenoxolone (CBX) or 1mM probenecid (PBD), two Panx channel inhibitors, and then incubated with rabbit anti-Panx1, anti-Panx2 and anti-Panx3 antibodies (1:200)...
April 10, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28390470/supplementation-of-culture-medium-with-l-carnitine-improves-the-development-and-cryotolerance-of-in-vitro-produced-porcine-embryos
#8
J L Lowe, L K Bartolac, R Bathgate, C G Grupen
Porcine oocytes and embryos contain substantial amounts of lipid, with little known regarding its metabolic role during development. This study investigated the role of lipid metabolism and the interaction between carbohydrate and lipid substrates in porcine embryos. Following in vitro fertilisation, presumptive zygotes were transferred to culture medium supplemented with L-carnitine, a co-factor required for the metabolism of fatty acids. In porcine zygote medium-3 (PZM-3), which contains pyruvate and lactate, 3mM L-carnitine was the only dose that improved cleavage rates compared with the control...
April 10, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28384430/low-doses-of-bisphenol-a-can-impair-postnatal-testicular-development-directly-without-affecting-hormonal-or-oxidative-stress-levels
#9
Fernanda M Ogo, Glaucia E M L Siervo, Géssica D Gonçalves, Rubens Cecchini, Flavia A Guarnier, Janete Ap Anselmo-Franci, Glaura S A Fernandes
Bisphenol A (BPA) is considered a potent endocrine disruptor, causing changes in the endocrine system due to its oestrogenic activity. Male individuals may be susceptible to endocrine, morphological and physiological alterations during testicular postnatal development. The aim of the present study was to evaluate whether exposure to BPA during the peripubertal period can damage testicular development. To this end, male Wistar rats were treated with BPA via gavage at doses of 20 or 200µgkg-1 on Postnatal Days (PND) 36-66...
April 7, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28380326/sex-specific-differences-and-developmental-programming-for-diseases-in-later-life
#10
Deepali P Sundrani, Suchitra S Roy, Anjali T Jadhav, Sadhana R Joshi
Epidemiological data indicate that developmental programming of various non-communicable diseases (NCDs) occurs as a consequence of altered maternal metabolic and physiological status due to a number of environmental insults during pregnancy. Sex-specific differences have also been reported in most NCDs. Evidence suggests that beginning from conception, the maternal and neonatal metabolic environment, including hormones, contributes to sex-specific placental development. The placenta then regulates the sex-specific differences in NCDs via the epigenetic mechanisms that are further affected by hormones...
April 6, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28376314/endogenous-lysophosphatidic-acid-participates-in-vascularisation-and-decidualisation-at-the-maternal-fetal-interface-in-the-rat
#11
Micaela S Sordelli, Jimena S Beltrame, Elsa Zotta, Natalia Gomez, Ganna Dmytrenko, María Elena Sales, Sandra M Blois, Carlos Davio, Silvina Perez Martinez, Ana M Franchi, María L Ribeiro
Lysophosphatidic acid (LPA) affects several female reproductive functions through G-protein-coupled receptors. LPA contributes to embryo implantation via the lysophospholipid LPA3 receptor. In the present study we investigated the participation of endogenous LPA signalling through the LPA3 receptor in vascularisation and decidualisation, two crucial events at the maternal-fetal interface. Pregnant rats were treated with diacylglycerol pyrophosphate (DGPP), a highly selective antagonist of LPA3 receptors, on Day 5 of gestation...
April 5, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28366192/melatonin-potentially-acts-directly-on-swine-ovary-by-modulating-granulosa-cell-function-and-angiogenesis
#12
Giuseppina Basini, Simona Bussolati, Roberta Ciccimarra, Francesca Grasselli
Melatonin exerts well-known reproductive effects, mainly acting on hypothalamic gonadotrophin-releasing hormone release. More recent data suggest that melatonin acts directly at the ovarian level, even if, at present, these aspects have been only partly investigated. Swine follicular fluid contains melatonin and its concentration is significantly reduced during follicular growth. Therefore, the present study was undertaken to examine the effects of melatonin, used at physiological concentrations, on cultured swine granulosa cells collected from small (<3mm) and large (>5mm) follicles on the main parameters of granulosa cell function such as proliferation and steroidogenesis, namely oestradiol 17β and progesterone (P4) production...
April 3, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28359014/sperm-motility-activation-in-the-critically-endangered-booroolong-frog-the-effect-of-medium-osmolality-and-phosphodiesterase-inhibitors
#13
Aimee J Silla, Leesa M Keogh, Phillip G Byrne
Effective activation of sperm motility is fundamental to successful artificial fertilisation; however, studies investigating optimal procedures in amphibians are lacking. This study found the optimal osmolality of activation media for sperm motility activation and evaluated the effect of phosphodiesterase (PDE) inhibitors on sperm activation and longevity in the critically endangered booroolong frog, Litoria booroolongensis. To assess the effect of medium osmolality (10, 25, 50, 75, 100 and 200mOsmolkg-1) and PDE inhibitors (control, 2...
March 31, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28356185/steroid-hormones-interact-with-natriuretic-peptide-c-to-delay-nuclear-maturation-to-maintain-oocyte-cumulus-communication-and-to-improve-the-quality-of-in-vitro-produced-embryos-in-cattle
#14
Ana Caroline S Soares, Valentina Lodde, Rodrigo G Barros, Christopher A Price, Alberto M Luciano, José Buratini
In vivo, oocyte maturation is triggered by the ovulatory LH surge, whereas in vitro it is precociously induced when the cumulus-oocyte complex is removed from the follicle. Natriuretic peptide C (NPPC) delays germinal vesicle breakdown (GVBD) while increasing oocyte-cumulus communication during in vitro maturation (IVM) in cattle. In the present study we first tested the hypothesis that steroids secreted by the follicle (17β-oestradiol, progesterone and androstenedione) interact with NPPC to delay GVBD and to maintain oocyte-cumulus communication as assessed by transfer of a dye (Lucifer Yellow) from the oocyte to cumulus cells...
March 30, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28356184/placentation-in-the-plains-zebra-equus-quagga
#15
W R Twink Allen, Fiona Stansfield, Sandra Wilsher
The placenta and fetal gonads of 12 pregnant plains zebra (Equus quagga), estimated to be between 81 and 239 days of gestation, were examined. The diffuse, microcotyledonary zebra placenta appeared, developmentally, to be 3-4 weeks behind its counterpart in horse pregnancy and this, together with the presence of small and long-lived endometrial cups, low levels of zebra chorionic gonadotrophin in maternal serum and few accessory corpora lutea in the maternal ovaries during the first half of gestation, made zebra pregnancy more similar to donkey than horse pregnancy...
March 30, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28356183/cryopreservation-of-saltwater-crocodile-crocodylus-porosus-spermatozoa
#16
S D Johnston, E Qualischefski, J Cooper, R McLeod, J Lever, B Nixon, A L Anderson, R Hobbs, J Gosálvez, C López-Fernández, T Keeley
The aim of the present study was to develop a protocol for the successful cryopreservation of Saltwater crocodile spermatozoa. Sperm cells were frozen above liquid nitrogen vapour in phosphate-buffered saline (PBS) containing either 0.3M trehalose, 0.3M raffinose or 0.3M sucrose and compared with glycerol (0.3-2.7M). Although the highest levels of mean post-thaw motility were observed following cryopreservation in 0.3M trehalose (7.6%) and 0.3M sucrose (7.3%), plasma membrane integrity (PI) was best following cryopreservation in 2...
March 30, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28343468/glutamine-protects-rabbit-spermatozoa-against-oxidative-stress-via-glutathione-synthesis-during-cryopreservation
#17
Zhendong Zhu, Xiaoteng Fan, Yinghua Lv, Yan Lin, De Wu, Wenxian Zeng
Mammalian spermatozoa are extremely susceptible to high doses of reactive oxygen species (ROS). The aim of the present study was to investigate the potential role of glutamine in protecting rabbit spermatozoa against ROS stress during cryopreservation and post-thaw incubation. Freshly ejaculated semen was diluted with Tris-citrate-glucose extender supplemented with glutamine. The addition of 20mM glutamine significantly improved sperm motility, acrosome integrity, membrane integrity and mitochondrial activity...
March 27, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28343467/successful-chimera-production-in-the-hungarian-goose-anser-anser-domestica-by-intracardiac-injection-of-blastodermal-cells-in-3-day-old-embryos
#18
Nikoletta Sztán, Bence Lázár, Nóra Bodzsár, Barbara Végi, Krisztina Liptói, Bertrand Pain, Eszter Patakiné Várkonyi
The conservation of genetic resources of avian species has become increasingly important over the past decade. The aim of the present study was to develop a genome preservation technique for the Hungarian goose Anser anser domestica. To this end, we developed a novel approach combining the simplicity of isolating a blastodermal cell suspension, which includes forming primordial germ cells (PGCs), with the efficiency of targeting future gonads by injecting these cells into the cardiac vein of the developing host embryo...
March 27, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28298247/tissue-specific-inhibition-of-urokinase-type-plasminogen-activator-expression-in-the-testes-of-mice-by-inducible-lentiviral-rna-interference-causes-male-infertility
#19
Kai Zhao, Yan Liu, Zhe Xiong, Lian Hu, Cheng-Liang Xiong
Urokinase-type plasminogen activator (uPA) is involved in many physiological processes, including male infertility. To explore the effects of uPA in male reproduction, we constructed an inducible uPA short hairpin RNA (shRNA) system expressed by lentiviral vectors. After proving inhibition of uPA expression in the mouse Sertoli cell line TM4 by 1μgmL-1 doxycycline (Dox), two lentivirus (pLenti4-shRNA and pLenti6/TR) were co-microinjected into mouse testes to produce TetR&shuPA mice model. Though oral gavage by 0...
March 16, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28298246/prostaglandin-f2-ptgfr-signalling-activation-growth-factor-expression-and-cell-proliferation-in-bovine-endometrial-explants
#20
Shuangyi Zhang, Bo Liu, Long Gao, Wei Mao, Changqi Fu, Duritahala, Nan Zhang, Ying Zhang, Yuan Shen, Jinshan Cao
The endometrium of domestic animals undergoes regular periods of regeneration and degeneration and exhibits a remarkable capacity for self-repair during the oestrous cycle. The endometrial growth pattern is also observed during in the implantation period and early pregnancy, but the mechanism underlying endometrial growth in these processes remains unclear. A positive correlation between endometrial growth in these processes and prostaglandin (PG) F2α secretion has been reported, but the roles that PGF2α plays in endometrial growth is less studied...
March 16, 2017: Reproduction, Fertility, and Development
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