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Molecular Reproduction and Development

Daniela Paes de Almeida Ferreira Braga, Edson Borges, Adriana Teixeira Godoy, Daniela Antunes Montani, Amanda Souza Setti, Bianca Ferrarini Zanetti, Rita de Cássia Sávio Figueira, Marcos Nogueira Eberlin, Edson Guimarães Lo Turco
For the present study we asked whether the endometrial fluid lipidomic may be a useful approach to predict endometrial receptivity in freeze-all cycles. For this case-control study, endometrial fluid samples were collected from 41 patients undergoing freeze-all cycles. Samples were split depending on the pregnancy outcome: Positive-Group (n=24) and Negative-Group (n=17). Data were acquired by the MALDI-TOF mass spectrometry. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were applied...
November 12, 2018: Molecular Reproduction and Development
Manxi Jiang, Liangshan Hu, Baoping Wang, Danxia Chen, Yahong Li, Zhen Zhang, Yan Zhu
Embryo implantation is a complicated event that relies on two critical factors: the competent blastocyst and the receptive uterus. Successful implantation results from tight coordination of these two factors. The maternal hormone environment of the uterus and molecular cross-talk between the embryo and uterine tissue play pivotal roles in implantation. Here we showed that regulator of G-protein signaling 2 (RGS2), a member of ubiquitous family of proteins that regulate G-protein activation, plays an important role in embryo implantation by interfering in the crosstalk between the embryo and uterine tissue...
November 9, 2018: Molecular Reproduction and Development
John Parrington, Christophe Arnoult, Rafael A Fissore
More than 15 years have elapsed since the identification of PLCzeta 1 (PLCζ) from a genomic search for mouse testis/sperm specific PLCs. This molecule was proposed to represent the sperm factor responsible for the initiation of calcium (Ca2+ ) oscillations required for egg activation and embryo development in mammals. Supporting evidence for this role emerged from studies documenting its expression in all mammals and other vertebrate species, the physiological Ca2+ rises induced by injection of its mRNA into mammalian and non-mammalian oocytes, and the lack of expression in infertile males that fail ICSI...
November 8, 2018: Molecular Reproduction and Development
Ling Yang, Qingkai Wang, Yong Liu, Leying Zhang, Wan Lv, Baoliang Liu
Lymph nodes are distributed all over the body and are part of the lymphatic system. The interferon-stimulated gene 15 kDa protein (ISG15) and prostaglandins (PGs) are involved in the establishment of pregnancy and are expressed in the uterus during early pregnancy in sheep. In this study, the ovine lymph nodes were obtained on day 16 of the estrous cycle, and days 13, 16 and 25 of pregnancy, and the expression of ISG15 and PG synthases, including cyclooxygenase 1 (COX-1), COX-2, PGE synthase (PTGES), and a PGF synthase (aldo-keto reductase family 1, member B1, AKR1B1), were detected by quantitative real-time PCR, Western blot analysis and immunohistochemistry analysis...
November 8, 2018: Molecular Reproduction and Development
Ye Young Shin, Eun-Jin Kang, Jea Sic Jeong, Min Jae Kim, Eui-Man Jung, Eui-Bae Jeung, Beum-Soo An
Many steroid hormones such as estrogen (E2) bind to their receptors for the regulation of biological processes. Pregnenolone (P5) is the precursor form of almost all steroid hormones and is often used to treat skin disorders and neurological complications. However, the mechanism and physiological function of P5 in reproductive organs are not well established. In this study, we investigated the effects of P5 on activation and expression of E2 receptor (ER) in the uteri and ovaries. To study the mechanism of P5 directly, Ishikawa cells were transfected with estrogen response element (ERE)-luciferase plasmid and isoforms of ER...
November 8, 2018: Molecular Reproduction and Development
Jitender Kumar Bhardwaj, Meenu Mittal, Priyanka Saraf
Pesticides are known to cause wide range of reproductive problems that possesses degenerative effects on mammalian fertility. Glyphosate (GLP), a broad-spectrum organophosphate herbicide, is known to be a potent mammalian toxicant. The present study aims at assessing the GLP (0.1, 2.0 and 4.0 mg/ml) induced granulosa cells toxicity and evaluating the mitigating effects of Vitamins C & E (0.5mM and 1.0mM) in healthy caprine antral follicles, cultured in vitro in dose and time (24, 48 and 72 hr) dependent manner and subjected to various cytotoxic and geno-toxic analysis namely classic histology, EB/AO differential staining, oxidative stress parameters and antioxidant enzymatic activity...
November 8, 2018: Molecular Reproduction and Development
Paula Tríbulo, Leandro Balzano-Nogueira, Ana Conesa, Luiz G Siqueira, Peter J Hansen
The uterine microenvironment during the first 7 days after ovulation accommodates and facilitates sperm transit to the oviduct and constitutes the sole source of nutrients required for development of preimplantation embryos. Knowledge of the composition of uterine fluid is largely incomplete. Using untargeted mass spectrometry, we characterized the uterine metabolome during the first 7 days of the estrous cycle. Bovine uteri were collected on day 0 (N=4), 3 (N=4), 5 (N=3) and 7 (N=4) relative to ovulation and flushed with Dulbecco's phosphate-buffered saline...
November 1, 2018: Molecular Reproduction and Development
C De Paepe, A Aberkane, D Dewandre, W Essahib, K Sermon, M Geens, G Verheyen, H Tournaye, H Van de Velde
Comprehensive understanding of lineage differentiation and apoptosis processes are important to increase our knowledge on human preimplantation development in vitro. We know that BMP signaling is important for different processes during mammalian development. In mouse preimplantation embryos BMP signaling has been shown to play a role in the differentiation into extra-embryonic trophectoderm (TE) and primitive endoderm (PE). In this study we aimed to investigate the effect of BMP4 supplementation to human preimplantation embryos cultured in vitro...
October 29, 2018: Molecular Reproduction and Development
Ying Liu, Yalan Yang, Wentong Li, Yanmin Zhang, Yanzhao Yang, Hua Li, Zhaoyu Geng, Hong Ao, Rong Zhou, Kui Li
Cumulus cells secreting steroid hormones have important functions in oocyte development. Several members of the short chain dehydrogenase/reductase (SDR) family are critical to the biosynthesis of steroid hormones. NADPH-dependent retinol dehydrogenase/reductase (NRDR), a member of the SDR superfamily, is overexpressed in pig breeds that also show high levels of androstenone. However, the potential functions and regulatory mechanisms of NRDR in pig ovaries have not been reported until now. The present study demonstrated that NRDR is highly expressed in pig ovaries and is specifically located in cumulus granulosa cells...
October 29, 2018: Molecular Reproduction and Development
Gongqing Liu, Yuan Li, Bing Du, Qi Sun, Wanjing Qi, Yuan Liu, Xue Zhang, Meiyu Jin, Zhihong Zheng
Previous studies have reported that only primordial follicles and empty follicles can be found in 7.5-days-postparturition (dpp) Sohlh1-/- mouse ovaries and females are infertility. There appears to be a defect in follicle development during the primordial-to-primary follicle transition in Sohlh1-/- mouse ovaries. However, detailed analyses of these phenomena have not been performed. In this study, we used Sohlh1-/- transgenic mice to explore the role of Sohlh1 in folliculogenesis. The results showed that only primordial follicles and empty follicles can be observed in Sohlh1-/- ovaries from 0...
October 25, 2018: Molecular Reproduction and Development
Won-Jae Kim, Seung-Eun Lee, Yun-Gwi Park, Sang-Gi Jeong, Eun-Young Kim, Se-Pill Park
The citrus flavonoid hesperetin has a variety of pharmacological actions, including antioxidant, anti-inflammatory, and anticancer activities. This study investigated whether hesperetin prevents aging of oocytes in vitro in which it determined the maturation of nuclear and cytoplasm and the developmental capacity of embryo by modulating the ROS level. Porcine oocytes were matured in vitro for 44 h (control) and for an additional 24 h in the presence of 0, 1, 10, 100, and 250 μM hesperetin (aging, H-1, H-10, H-100, and H-250, respectively)...
October 24, 2018: Molecular Reproduction and Development
Daniela Bebbere, Francesca Mossa, Fabiola Paula-Lopes, Alan O'Doherty
The modern definition of Epigenetics refers to heritable changes in gene function, and in turn in phenotypes, that are not caused by genetic alterations. It comprises DNA methylation, histone modifications and non coding RNAs that collectively compose the "epigenome" and orchestrates numerous essential regulatory mechanisms during mammalian development, including cell and tissue differentiation, genomic imprinting and X chromosome inactivation. This article is protected by copyright. All rights reserved...
October 23, 2018: Molecular Reproduction and Development
Luciana M Sari, Renato Zampini, Martin E Argañaraz, María I Carretero, Fernanda G Fumuso, Daniela E Barraza, Marcelo Ratto, Silvana A Apichela
β-Nerve Growth Factor (β-NGF) is a seminal plasma element, responsible for inducing ovulation in camelids. The main organ of β-NGF production remains nondescript. The aims of this study were: (1) characterize gene expression and protein localization of β-NGF and its main receptor TrKA in the llama male reproductive tract, and (2) determine whether the seminal β-NGF interacts with ejaculated sperm by localizing β-NGF and TrKA in epididymal, ejaculated, and acrosome reacted (AR) sperms and, additionally, by identifying β-NGF presence in sperm-adsorbed proteins (SAP)...
October 16, 2018: Molecular Reproduction and Development
Anna A Pendina, Olga A Efimova, Mikhail I Krapivin, Irina D Mekina, Andrei V Tikhonov, Alla S Koltsova, Anastasiia V Petrovskaia-Kaminskaia, Olga G Chiryaeva, Igor Yu Kogan, Alexander M Gzgzyan, Vladislav S Baranov
5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) are derivatives of 5-methylcytosine (5mC). Sequential enzymatic oxidation of 5mC produces 5-hydroxymethylcytosine (5hmC), 5fC, and 5caC. Similarly to 5hmC, 5fC and 5caC serve as transient intermediates in active DNA demethylation which is a crucial step in epigenetic reprogramming of mammalian preimplantation embryos. This article is protected by copyright. All rights reserved.
October 15, 2018: Molecular Reproduction and Development
Johanna Bennemann, Hanna Grothmann, Christine Wrenzycki
Preimplantation epigenetic reprogramming is sensitive to the environment of the gametes and the embryo. In vitro maturation (IVM) of bovine oocytes is a critical step of embryo in vitro production (IVP) procedures and several factors influence its efficiency, including atmospheric oxygen tension. The possibility that the IVM environment can alter this process is tested by determining whether the global DNA methylation pattern (measured via immunofluorescent labelling of 5-methylcytosine, 5meC) in the parental pronuclei of bovine zygotes produced from cumulus-oocyte-complexes (COC) matured under low (5%) and atmospheric (~20%) oxygen tension...
October 11, 2018: Molecular Reproduction and Development
Augusto Weber, Jayse Alves, Ana Lucia Abujamra, Ivan Cunha Bustamante-Filho
Human secondary hypogonadism is associated with impaired testicular function, however, little is known about its impact on sperm epididymal maturation. Endocrine disruption in the epididymis could impair the secretion of key proteins, such as β-defensins, responsible for spermatozoa maturation during epididymal transit. This study evaluated the sequence and structural similarities between porcine epididymal β-defensins pBD3, pBD4, pBD125, and pEP2C and their human homologs using bioinformatics integrated with information derived from protein databanks...
October 11, 2018: Molecular Reproduction and Development
Xin Zhang, Jialong Shi, Yulong Sun, Youfang Zhu, Ziping Zhang, Yilei Wang
In present study, the next-generation sequencing technology was employed to development a transcriptome database of gonad and liver from 3-year-old male and female Amur sturgeon (Acipenser schrenckii). A total 139,406 unigenes were generated after the Illumina Hiseq. 2500 sequence and assembled by Trinity. The differential expression analysis between male and female obtained 5,199 differentially expressed genes (DEGs) in gonad and 457 DEGs in liver. GO enrich analysis showed that the specific DEGs of gonad play a dominant role in reproductive processes...
October 10, 2018: Molecular Reproduction and Development
Laura Michalovic, Luke Currin, Karina Gutierrez, Anne-Marie Bellefleur, Werner G Glanzner, Yasmin Schuermann, Mariana P de Macedo, Rodrigo C Bohrer, Naomi Dicks, Rosalba Lopez, Milena Taibi, Ejimedo Madogwe, Audrey St-Yves, Rafael G Mondadori, Jim Gourdon, Christian Vigneault, Hernan Baldassarre, Vilceu Bordignon
Oocytes collected from prepubertal animals are known to be less developmentally competent than those from adult animals. There is evidence suggesting that acquisition of developmental competence in bovine oocytes may be linked to the expression profile of genes in the granulosa cells (GCs). Cumulus-oocyte complexes (COC) and GCs were collected from 12 Holstein heifers between 2 and 6 months of age (nine follicle-stimulating hormone [FSH] treated and three untreated) and eight FSH-treated cows. The COCs from prepubertal animals were matured, fertilized, and cultured in vitro to assess development to the blastocyst stage...
October 9, 2018: Molecular Reproduction and Development
Mingcan Zhou, Xiwei Jia, Haifu Wan, Shuhong Wang, Xin Zhang, Ziping Zhang, Yilei Wang
Mud crab Scylla paramamosain is an important commercially species widely cultured in China. It is well known that the eyestalk regulates reproductive activities in crustacean. In our previous research, we found that miR-34 expression level in male eyestalk was significantly higher than that in female's. Thus, we assumed it may play an important role in reproduction regulation. In this study, we utilized bioinformatic tools to identify target genes of miR-34 in eyestalk. Six reproduction-related genes with intact 3'-UTR, including molt-inhibiting hormone (MIH), crustacean hyperglycemic hormone (CHH), vitellogenesis-inhibiting hormone (VIH), red pigment concentrating hormone (RPCH), ecdysone receptor (EcR), and farnesoic acid methyltransferase (FAMeT) were identified...
October 4, 2018: Molecular Reproduction and Development
Debabrata Das, Reshef Tal
No abstract text is available yet for this article.
September 19, 2018: Molecular Reproduction and Development
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