journal
MENU ▼
Read by QxMD icon Read
search

Molecular Reproduction and Development

journal
https://www.readbyqxmd.com/read/28079292/germ-cell-tumors-insights-from-the-drosophila-ovary-and-the-mouse-testis
#1
REVIEW
Helen K Salz, Emily P Dawson, Jason D Heaney
Ovarian and testicular germ cell tumors of young adults are thought to arise from defects in germ cell development, but the molecular mechanisms underlying malignant transformation are poorly understood. In this review, we focus on the biology of germ cell tumor formation in the Drosophila ovary and the mouse testis, for which the evidence supports common underlying mechanisms such as blocking initiation into the differentiation pathway, impaired lineage progression, and sexual identity instability. We then discuss how these concepts inform our understanding of the disease in humans...
January 12, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28067447/importance-of-eif2%C3%AE-phosphorylation-as-a-protective-mechanism-against-heat-stress-in-mouse-male-germ-cells
#2
Jungbin Yoon, Kyosun Park, Deog Su Hwang, Kunsoo Rhee
Mammalian male germ cells are exceptionally labile to heat stress. A temporal arrest of translation is one immediate response to heat, which involves heat-induced phosphorylation of eukaryotic initiation factor 2α (eIF2α) to block the formation of the translational initiation complex. Here, we investigated the protective mechanisms against heat stress in mouse male germ cells. All known eIF2α kinases were expressed in lineage- and developmental stage-specific manners in the testis; noteworthy was the presence of Gcn2 (General control nonderepressible 2 kinase) in spermatocytes of all seminiferous tubules...
January 9, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28044390/systematic-in-vitro-and-in-vivo-characterization-of-leukemia-inhibiting-factor-and-fibroblast-growth-factor-derived-porcine-induced-pluripotent-stem-cells
#3
Jan O Secher, Ahmet Ceylan, Gianluca Mazzoni, Kaveh Mashayekhi, Tong Li, Suchitra Muenthaisong, Troels T Nielsen, Dong Li, Shengting Li, Stoyan Petkov, Susanna Cirera, Yonglun Luo, Lori Thombs, Haja N Kadarmideen, Andras Dinnyes, Lars Bolund, Bernard A J Roelen, Mette Schmidt, Henrik Callesen, Poul Hyttel, Kristine K Freude
Derivation and stable maintenance of porcine induced pluripotent stem cells (piPSCs) is challenging. We herein systematically analyzed two piPSC lines, derived by lentiviral transduction and cultured under either leukemia inhibitory factor (LIF) or fibroblast growth factor (FGF) conditions, to shed more light on the underlying biological mechanisms of porcine pluripotency. LIF-derived piPSCs were more successful than their FGF-derived counterparts in the generation of in vitro chimeras and in teratoma formation...
January 3, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28032683/the-varied-ways-of-being-male-and-female
#4
REVIEW
Lukas Schärer
Our understanding of sexual reproduction is mainly informed by research on gonochorists (i.e. species with separate sexes), including insects, birds, and mammals. But the male and female sexes are not two types of individuals; they actually represent two different reproductive strategies, and in many organisms these two strategies are distributed among individuals in a population in a variety of ways. For example, sequential hermaphrodites (or sex-changers) exhibit one strategy early in life, and later switch to the other, while simultaneous hermaphrodites exhibit both strategies at the same time...
December 29, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28029195/expression-of-tamoxifen-inducible-cre-recombinase-in-lcn5-creer-t2-transgenic-mouse-caput-epididymis
#5
Juan Xu, Guangxin Yao, Yanfei Ru, Shengsong Xie
The epididymis, which connects the testis to vas deferens, plays a crucial role regulating sperm maturation and fertilization. Here, a tamoxifen-inducible CreER(T2) recombinase transgenic mouse was generated to study the function of genes in the caput epididymis using the Cre/LoxP system, which is driven by the 1.8-kb Lcn5 promoter (Lcn5-CreER(T2) ). Both CRE recombinase and ER(T2) mRNA were specifically expressed in the caput epididymis, beginning at postnatal Day 30 and increasing thereafter. Crossing these Lcn5-CreER(T2) transgenic mice with Rosa26;mT/mG reporter mice, which express membrane-bound GFP (mGFP) only after CRE is active at its genetic locus, resulted in the presence of GFP only in the middle/distal caput epididymis after tamoxifen induction...
December 28, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28029191/beauty-in-the-breakdown-morphology-of-the-first-polar-body-in-mature-mouse-eggs
#6
Allison R Grover, Dana Kimelman, Megan A Runge, Francesca E Duncan
In vitro maturation (IVM) is the process whereby oocytes arrested in prophase of meiosis I are stimulated to resume meiosis and progress to metaphase of meiosis II (MII) ex vivo. Here we examined the cytoskeletal morphology of mature MII eggs obtained following IVM. Cumulus oocyte complexes were isolated from antral follicles of hyper-stimulated female mice, and cumulus cells were removed to induce spontaneous oocyte meiotic maturation. This article is protected by copyright. All rights reserved.
December 28, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28004452/inhibitory-mechanism-of-l-glutamic-acid-on-spawning-of-the-starfish-patiria-asterina-pectinifera
#7
Masatoshi Mita
L-Glutamic acid was previously identified as an inhibitor of spawning in the starfish Patiria (Asterina) pectinifera; this study examined how L-glutamic acid works. Oocyte release from ovaries of P. pectinifera occurred after germinal vesicle breakdown (GVBD) and follicular envelope breakdown (FEBD) when gonads were incubated ex vivo with either relaxin-like gonad-stimulating peptide (RGP) or 1-methyladenine (1-MeAde). L-Glutamic acid blocked this spawning phenotype, causing the mature oocytes to remain within the ovaries...
December 22, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27990711/a-novel-functional-variant-in-wilms-tumor-1-wt1-is-associated-with-idiopathic-non-obstructive-azoospermia
#8
Jin Xu, Lile Jiang, Wenzhu Yu, Haibin Guo, Helong Zhang, Duo Wei, Lingling Liang, Ke Feng, Xiaobing Song, Qi Liu, Bingbing Song, Haoying Hao, Ying Zhang, Cuilian Zhang
Idiopathic non-obstructive azoospermia (INOA) is one of the most severe forms of male infertility, yet its pathophysiology remains unclear. WT1 (Wilm's tumor 1) regulates the polarity of Sertoli cells, thereby playing a critical, indirect role in spermatogenesis. Here, we evaluated WT1 gene variation associates with INOA by assessing its promoter and coding regions in 200 patients diagnosed with INOA and 200 proven fertile men. Three novel variants in the WT1 coding region were detected only in INOA patients, including two synonymous variants and one missense variant, p...
December 19, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27987309/regucalcin-is-widely-distributed-in-the-male-reproductive-tract-and-exerts-a-suppressive-effect-on-in-vitro-sperm-capacitation-in-the-water-buffalo-bubalus-bubalis
#9
LETTER
Harikrishna P, A M Shende, Mehtab S Parmar, Anjaneya A, Sreela L, Kumaresan A, Taru Sharma G, S K Bhure
Regucalcin is a multi-functional, calcium-binding protein with roles in calcium homeostasis, apoptosis, cell proliferation, and free radical neutralization. Regucalcin is broadly expressed in the male reproductive organs of rat and bovine; here, we report its expression in the reproductive tract of male buffalo - especially in testis, epididymis, seminal vesicle, prostate, and bulbourethral gland of buffalo, as analyzed by real-time PCR, Western blot, and immunolocalization. Regucalcin degradation in seminal plasma, despite its high abundance in vesicular fluid, was demonstrated using recombinant regucalcin co-incubated with buffalo seminal plasma...
December 17, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27935143/the-use-of-c57bl-6xcba-f1-hybrid-cross-as-a-model-for-human-age-related-oocyte-aneuploidy
#10
LETTER
Nicole J Camlin, Eileen A McLaughlin, Janet E Holt
Oocyte numbers decrease whereas the incidence of aneuploidy increases as women age. The molecular mechanisms underpinning this age-related decline in oocyte quality are not completely understood. Human oocytes are particularly error prone, with reports of aneuploidy rates as high as 50-60% (Fragouli et al. 2011; Kuliev et al. 2011). This article is protected by copyright. All rights reserved.
December 9, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27883267/translocation-of-tektin-3-to-the-equatorial-segment-of-heads-in-bull-spermatozoa-exposed-to-dibutyryl-camp-and-calyculin-a
#11
Mariko Tsukamoto, Erina Hiyama, Karen Hirotani, Takafumi Gotoh, Tetsuichiro Inai, Hiroshi Iida
Tektins (TEKTs) are filamentous proteins associated with microtubules in cilia, flagella, basal bodies, and centrioles. Five TEKTs (TEKT1, -2, -3, -4, and -5) have been identified as components of mammalian sperm flagella. We previously reported that TKET1 and -3 are also present in the heads of rodent spermatozoa. The present study clearly demonstrates that TEKT2 is present at the acrosome cap whereas TEKT3 resides just beneath the plasma membrane of the post-acrosomal region of sperm heads in unactivated bull spermatozoa, and builds on the distributional differences of TEKT1, -2, and -3 on sperm heads...
November 23, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27879032/altered-expression-of-brg1-and-histone-demethylases-and-aberrant-h3k4-methylation-in-less-developmentally-competent-embryos-at-the-time-of-embryonic-genome-activation
#12
Werner G Glanzner, Audrey Wachter, Ana Rita S Coutinho, Marcelo S Albornoz, Raj Duggavathi, Paulo B D GonÇAlves, Vilceu Bordignon
Epigenetics is a fundamental regulator underlying many biological functions, such as development and cell differentiation. Epigenetic modifications affect key chromatin regulation, including transcription and DNA repair, which are critical for normal embryo development. In this study, we profiled the expression of epigenetic modifiers and patterns of epigenetic changes in porcine embryos around the period of embryonic genome activation (EGA). We observed that Brahma-related gene 1 (BRG1) and Lysine demethylase 1A (KDM1A), which can alter the methylation status of lysine 4 in histone 3 (H3K4), localize to the nucleus at Day 3-4 of development...
November 23, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27879029/progesterone-receptor-and-prostaglandins-mediate-luteinizing-hormone-induced-changes-in-messenger-rnas-for-adamts-proteases-in-theca-cells-of-bovine-periovulatory-follicles
#13
E L Willis, P J Bridges, J E Fortune
Little is known about the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family of extracellular proteases in ovarian follicles of non-rodent species, particularly in theca cells. In the present study, temporal patterns of change in mRNA for four ADAMTS subtypes and the hormonal transcriptional regulation of two subtypes were investigated in theca cells during the periovulatory period in cattle. Follicles were obtained at 0 h post-gonadotropin-releasing hormone (GnRH; preovulatory) or at 6, 12, 18, or 24 h after injection of GnRH (periovulatory) to induce a luteinizing hormone (LH)/follicle-stimulating hormone surge...
November 23, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27864906/role-and-regulation-of-glycogen-synthase-kinase-3-beta-in-bovine-spermatozoa
#14
Michael Belenky, Haim Breitbart
The serine/threonine kinase Glycogen synthase kinase 3 (GSK-3) is a master switch that regulates a multitude of cellular pathways, including the acrosome reaction in sperm. In epididymal sperm cells, for example, GSK-3 activity correlates with inhibition of motility-yet no direct pathways connecting GSK-3 activation with loss of motility have been described. Indeed, the details of how GSK-3 is regulated during sperm capacitation and the acrosome reaction remains obscure. To this end, we addressed the involvement of the GSK-3 beta isoform in several known pathways that contribute to motility and the acrosome reaction...
November 19, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27864905/polyacrylamide-gel-as-a-culture-substrate-improves-in-vitro-oocyte-growth-from-porcine-early-antral-follicles
#15
Yasuhisa Munakata, Ryouka Kawahara-Miki, Koumei Shirasuna, Takehito Kuwayama, Hisataka Iwata
A major difference between in vivo and in vitro follicle culture is the stiffness of the substrate in which the follicles grow. In this study, we examined the effect of polyacrylamide gel (PAG), as a culture substrate, on the development of porcine oocytes derived from early antral follicles. Oocyte-granulosa cell complexes (OGCs) were collected from the early antral follicles of gilts, and incubated individually for 14 days in a 96-well culture plate with or without PAG. We then evaluated the number of granulosa cells present in OGCs as well as the abundance of ATP, lipid, and acetylated lysine in oocytes...
November 19, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27862569/fibroblast-growth-factor-10-markedly-improves-in-vitro-maturation-of-porcine-cumulus-oocyte-complexes
#16
Yeo-Jin Son, Seung-Eun Lee, Hyuk Hyun, Min-Young Shin, Yun-Gwi Park, Sang-Gi Jeong, Eun-Young Kim, Se-Pill Park
Growth factors synthesized by ovarian somatic cells affect cumulus cell expansion and oocyte maturation in vitro. Fibroblast growth factor 10 (FGF10), for example, is a known regulator of mammalian cumulus-oocyte complex maturation. In this study, we investigated the effects of 0, 5, 10, 50, and 100 ng/mL FGF10 (5F, 10F, 50F, and 100F, respectively) on in vitro cumulus cell expansion, oocyte maturation, and embryo development. The percentage of fully expanded cumulus cells at the oocyte's metaphase-II (MII) stage was significantly higher in the 10F-treated group than in the control...
November 11, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27893173/bmp15l-figla-smc1bl-and-larp6l-are-preferentially-expressed-in-germ-cells-in-atlantic-salmon-salmo-salar-l
#17
Lene Kleppe, Rolf Brudvik Edvardsen, Tomasz Furmanek, Eva Andersson, Amélie Juanchich, Anna Wargelius
Atlantic salmon is a valuable commercial aquaculture species that would benefit economically and environmentally by controlling precocious puberty and preventing escapees from reproducing with wild populations. One solution to both these challenges is the production of sterile individuals by inhibiting the formation of germ cells, but achieving this requires more information on the specific factors that control germ cell formation. Here, we identified and characterized novel factors that are preferentially expressed in Atlantic salmon germ cells by screening for gonad-specific genes using available adult multi-tissue transcriptomes...
November 7, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27977056/toxic-embryos-oh-my%C3%A2
#18
EDITORIAL
Gary M Wessel
No abstract text is available yet for this article.
December 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27977055/erratum-zebrafish-vasa-is-required-for-germ-cell-differentiation-and-maintenance
#19
O Hartung, M M Forbes, F L Marlow
No abstract text is available yet for this article.
December 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27977054/shaping-the-drosophila-egg
#20
Audrey M Williams, Sally Horne-Badovinac
No abstract text is available yet for this article.
December 2016: Molecular Reproduction and Development
journal
journal
30049
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"