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Molecular Reproduction and Development

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https://www.readbyqxmd.com/read/27883267/translocation-of-tektin-3-to-the-equatorial-segment-of-heads-in-bull-spermatozoa-exposed-to-dibutyryl-camp-and-calyculin-a
#1
Mariko Tsukamoto, Erina Hiyama, Karen Hirotani, Takafumi Gotoh, Tetsuichiro Inai, Hiroshi Iida
Tektins (TEKTs) are filamentous proteins associated with microtubules in cilia, flagella, basal bodies, and centrioles. Five TEKTs (TEKT1, -2, -3, -4, and -5) have been identified as components of mammalian sperm flagella. We previously reported that TKET1 and TEKT3 are also present in the heads of rodent spermatozoa. The present study clearly demonstrates that TEKT2 is present at the acrosome cap whereas TEKT3 resides just beneath the plasma membrane of the post-acrosomal region of sperm heads in unactivated bull spermatozoa, and builds on the distributional differences of TEKT1, -2, and -3 on sperm heads...
November 23, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27879032/altered-expression-of-brg1-and-histone-demethylases-and-aberrant-h3k4-methylation-in-less-developmentally-competent-embryos-at-the-time-of-embryonic-genome-activation
#2
W G Glanzner, A Wachter, A R S Coutinho, M S Albornoz, R Duggavathi, P B D Gonçalves, V Bordignon
Epigenetics is a fundamental regulator underlying many biological functions, such as development and cell differentiation. Epigenetic modifications affect key chromatin regulation, including transcription and DNA repair, which are critical for normal embryo development. In this study, we profiled the expression of epigenetic modifiers and patterns of epigenetic changes in porcine embryos around the period of embryonic genome activation (EGA). We observed that Brahma-related gene 1 (BRG1) and Lysine demethylase 1A (KDM1A), which can alter the methylation status of lysine 4 in histone 3 (H3K4), localize to the nucleus at Days 3-4 of development...
November 23, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27879029/progesterone-receptor-and-prostaglandins-mediate-luteinizing-hormone-induced-changes-in-messenger-rnas-for-adamts-proteases-in-theca-cells-of-bovine-periovulatory-follicles
#3
E L Willis, P J Bridges, J E Fortune
Little is known about the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family of extracellular proteases in ovarian follicles of non-rodent species, particularly in theca cells. In the present study, temporal patterns of change in mRNA for four ADAMTS subtypes and the hormonal transcriptional regulation of two subtypes were investigated in theca cells during the periovulatory period in cattle. Follicles were obtained at 0 h post-gonadotropin-releasing hormone (GnRH; preovulatory) or at 6, 12, 18, or 24 h after injection of GnRH (periovulatory) to induce a luteinizing hormone (LH)/follicle-stimulating hormone surge...
November 23, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27864906/role-and-regulation-of-glycogen-synthase-kinase-3-beta-in-bovine-spermatozoa
#4
Michael Belenky, Haim Breitbart
The serine/threonine kinase Glycogen synthase kinase 3 (GSK-3) is a master switch that regulates a multitude of cellular pathways, including the acrosome reaction in sperm. In epididymal sperm cells, for example, GSK-3 activity correlates with inhibition of motility-yet no direct pathways connecting GSK-3 activation with loss of motility have been described. Indeed, the details of how GSK-3 is regulated during sperm capacitation and the acrosome reaction remains obscure. To this end, we addressed the involvement of the GSK-3 beta isoform in several known pathways that contribute to motility and the acrosome reaction...
November 19, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27864905/polyacrylamide-gel-as-a-culture-substrate-improves-in-vitro-oocyte-growth-from-porcine-early-antral-follicles
#5
Yasuhisa Munakata, Ryouka Kawahara-Miki, Koumei Shirasuna, Takehito Kuwayama, Hisataka Iwata
A major difference between in vivo and in vitro follicle culture is the stiffness of the substrate in which the follicles grow. In this study, we examined the effect of polyacrylamide gel (PAG), as a culture substrate, on the development of porcine oocytes derived from early antral follicles. Oocyte-granulosa cell complexes (OGCs) were collected from the early antral follicles of gilts, and incubated individually for 14 days in a 96-well culture plate with or without PAG. We then evaluated the number of granulosa cells present in OGCs as well as the abundance of ATP, lipid, and acetylated lysine in oocytes...
November 19, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27859831/albinism-as-a-visual-in-vivo-guide-for-crispr-cas9-functionality-in-the-sea-urchin-embryo
#6
Nathalie Oulhen, Gary M Wessel
No abstract text is available yet for this article.
November 15, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27862569/fibroblast-growth-factor-10-markedly-improves-in-vitro-maturation-of-porcine-cumulus-oocyte-complexes
#7
Yeo-Jin Son, Seung-Eun Lee, Hyuk Hyun, Min-Young Shin, Yun-Gwi Park, Sang-Gi Jeong, Eun-Young Kim, Se-Pill Park
Growth factors synthesized by ovarian somatic cells affect cumulus cell expansion and oocyte maturation in vitro. Fibroblast growth factor 10 (FGF10), for example, is a known regulator of mammalian cumulus-oocyte complex maturation. In this study, we investigated the effects of 0, 5, 10, 50, and 100 ng/mL FGF10 (5F, 10F, 50F, and 100F, respectively) on in vitro cumulus cell expansion, oocyte maturation, and embryo development. The percentage of fully expanded cumulus cells at the oocyte's metaphase-II (MII) stage was significantly higher in the 10F-treated group than in the control...
November 11, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27893173/bmp15l-figla-smc1bl-and-larp6l-are-preferentially-expressed-in-germ-cells-in-atlantic-salmon-salmo-salar-l
#8
Lene Kleppe, Rolf Brudvik Edvardsen, Tomasz Furmanek, Eva Andersson, Amélie Juanchich, Anna Wargelius
Atlantic salmon is a valuable commercial aquaculture species that would benefit economically and environmentally by controlling precocious puberty and preventing escapees from reproducing with wild populations. One solution to both these challenges is the production of sterile individuals by inhibiting the formation of germ cells, but achieving this requires more information on the specific factors that control germ cell formation. Here, we identified and characterized novel factors that are preferentially expressed in Atlantic salmon germ cells by screening for gonad-specific genes using available adult multi-tissue transcriptomes...
November 7, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27770611/creb-activity-is-required-for-luteinizing-hormone-induced-the-expression-of-egf-like-factors
#9
Yakun Wang, Xiaoqiong Hao, Jing Yang, Jia Li, Meijia Zhang
A surge of luteinizing hormone (LH) from the pituitary gland induces the expression of the epidermal growth factor (EGF)-like factors, which triggers oocyte maturation, cumulus expansion, and ovulation. How LH induces EGF-like factor expression is unclear. In the present study, a rapid increase of phosphorylated cAMP response element binding protein (CREB) was observed after the activation of LH receptor by human chorionic gonadotropin. Large antral follicles from equine chorionic gonadotropin-primed mice were cultured in medium with LH to stimulate the expression of EGF-like factors...
October 22, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27770608/non-canonical-expression-patterns-and-evolutionary-rates-of-sex-biased-genes-in-a-seasonal-fish
#10
M P Machado, I Matos, A R Grosso, M Schartl, M M Coelho
Sex determination is a highly variable process that utilizes many different mechanisms to initiate the cascade of differentiation processes. The molecular pathways controlling sexual development are less conserved than previously assumed, and appear to require active maintenance in some species; indeed, the developmental decision of gonad phenotype in gonochoristic species is not fixed at an early developmental stage. Much of the knowledge about sex determination mechanisms was derived from research on gonochoristic, non-seasonal breeders...
October 22, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27764533/signaling-events-associated-with-gonadotropin-releasing-hormone-agonist-induced-hormonal-castration-and-its-reversal-in-canines
#11
Ahmed Bulldan, Mazen Shihan, Sandra Goericke-Pesch, Georgios Scheiner-Bobis
A gonadotropin-releasing hormone agonist (GnRH-A) implant induces hormonal castration in dogs that is associated with reduced prostate and testes size. We address the molecular events associated with hormonal castration by examining GnRH-A effects on expression and phosphorylation of a number of key signaling proteins. Male beagles were treated for 5 months with a GnRH-A implant, and then surgically castrated at 0, 3, 6, 12, and 24 weeks after implant removal; untreated animals served as controls. GnRH-A treatment led to activation of c-Raf, Erk1/2, and p53 in the testes...
October 20, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27739607/multiple-roles-of-foxj3-in-spermatogenesis-a-lesson-from-foxj3-conditional-knockout-mouse-models
#12
Lili Ni, Hongchang Xie, Li Tan
The transcription factor FOXJ3 (Forkhead box J3) is highly expressed in spermatogonia and meiotic spermatocytes within mouse testes. Here, we addressed how FOXJ3 might participate in spermatogenesis using two conditional knockout mouse models in which the Foxj3 was deleted from either spermatogonia or meiotic spermatocytes. Both models exhibited complete male sterility, but distinct etiologies: Deleting FOXJ3 from spermatogonia using Foxj3(flox/flox) , Mvh-Cre mice caused Sertoli-cell-only syndrome in males...
October 14, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27704662/cigarette-smoking-alters-sialylation-in-the-fallopian-tube-of-women-with-implications-for-the-pathogenesis-of-ectopic-pregnancy
#13
Junko Nio-Kobayashi, Hazirah B Z Abidin, Jeremy K Brown, Toshihiko Iwanaga, Andrew W Horne, W Colin Duncan
Sialylation creates a negative charge on the cell surface that can interfere with blastocyst implantation. For example, α2,6-sialylation on terminal galactose, catalyzed by the sialyltransferase ST6GAL1, inhibits the binding of galectin-1, a β-galactoside-binding lectin. We recently reported the potential involvement of galectin-1 and -3 in the pathogenesis of tubal ectopic pregnancy; however, the precise role of galectins and their ligand glycoconjugates remain unclear. Here, we investigated the expression of the genes encoding α2,3- and α2,6-galactoside sialyltransferases (ST3GAL1-6 and ST6GAL1-2) and the localization of sialic acids in the Fallopian tube of women with or without ectopic implantation...
October 5, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27699901/model-of-the-delayed-translation-of-cyclin-b-maternal-mrna-after-sea-urchin-fertilization
#14
Vincent Picard, Odile Mulner-Lorillon, Jérémie Bourdon, Julia Morales, Patrick Cormier, Anne Siegel, Robert Bellé
Sea urchin eggs exhibit a cap-dependent increase in protein synthesis within minutes after fertilization. This rise in protein synthesis occurs at a constant rate for a great number of proteins translated from the different available mRNAs. Surprisingly, we found that cyclin B, a major cell-cycle regulator, follows a synthesis pattern that is distinct from the global protein population, so we developed a mathematical model to analyze this dissimilarity in biosynthesis kinetic patterns. The model includes two pathways for cyclin B mRNA entry into the translational machinery: one from immediately available mRNA (mRNAcyclinB) and one from mRNA activated solely after fertilization (XXmRNAcyclinB)...
October 4, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27676172/novel-sperm-and-gonadotropin-releasing-hormone-based-recombinant-fusion-protein-achievement-of-100-contraceptive-efficacy-by-co-immunization-of-male-and-female-mice
#15
Vidisha Minhas, Abhinav Shrestha, Neerja Wadhwa, Rita Singh, Satish Kumar Gupta
Improvements in long-term female contraception can be achieved by vaccinating with sperm-derived proteins. Here, recombinant proteins comprising either (i) N- (amino acid residues 1-80) or C- (amino acid residues 76-126) terminal fragments of mouse sperm protein 17 (Sp17) fused to the promiscuous T non-B cell epitope of tetanus toxoid (TT), amino acid residues 830-844 followed by di-lysine linker (KK) (TT-KK-Sp17N or TT-KK-Sp17C , respectively) or (ii) mouse equatorin (amino acid residues 21-185) fused to the T non-B cell epitope of bovine RNase (amino acid residues 94-104) were expressed in Escherichia coli...
September 27, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27870255/now-that-is-a-game-changer-the-entire-reproductive-cycle-of-an-oocyte-in-a-dish
#16
EDITORIAL
Gary M Wessel
No abstract text is available yet for this article.
November 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27696585/loss-of-function-of-kif1b-impairs-oocyte-meiotic-maturation-and-early-embryonic-development-in-mice
#17
Xiang-Wei Kong, Dong-Hui Wang, Cheng-Jie Zhou, Hong-Xia Zhou, Cheng-Guang Liang
Kinesin family member 1B (KIF1B) is an important microtubule-dependent monomeric motor in mammals, although little is known about its role in meiosis. We profiled KIF1B expression and localization during oocyte maturation and early embryonic development in mice, revealing a dynamic pattern throughout meiotic progression. Depletion or inhibition of KIF1B leads to abnormal polar body extrusion, disordered spindle dynamics, defects in chromosome congression, increased aneuploidy, and impaired embryonic development...
November 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27696582/ror2-in-the-adult-mouse-testis
#18
Ripla Arora, Diana J Laird
No abstract text is available yet for this article.
November 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27653174/protective-effects-of-melatonin-on-bovine-sperm-characteristics-and-subsequent-in-vitro-embryo-development
#19
Yun-Wei Pang, Ye-Qing Sun, Xiao-Long Jiang, Zi-Qiang Huang, Shan-Jiang Zhao, Wei-Hua Du, Hai-Sheng Hao, Xue-Ming Zhao, Hua-Bin Zhu
We aimed to investigate the effect of melatonin on bovine frozen-thawed semen and its impact on fertilization outcome. Plasma membrane integrity, mitochondrial activity, acrosome integrity, and levels of intracellular reactive oxygen species (ROS) were measured in spermatozoa treated with different concentrations of melatonin. Melatonin-treated spermatozoa were then used for in vitro fertilization, followed by analysis of subsequent embryo development and the expression of apoptosis- and antioxidant-related genes...
November 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27649385/embryonic-stem-like-cells-from-rabbit-blastocysts-cultured-with-melatonin-could-differentiate-into-three-germ-layers-in-vitro-and-in-vivo
#20
Ruxue Wei, Xueming Zhao, Haisheng Hao, Weihua Du, Huabin Zhu
The rabbit is considered an important model animal from which to obtain embryonic stem cells because of the utility of this animal in physiology and reproductive research. Here, we derived rabbit ES-like (rES-like) cells from blastocysts of superovulated Japanese white rabbits using culture medium containing 10(-7)  M melatonin, 10 ng/mL basic fibroblast growth factor, and 1,000 IU/mL human leukemia inhibitory factor. This concentration of melatonin had the most significant positive effects on the proliferation inner cell mass-derived cells (improving rates from 19...
November 2016: Molecular Reproduction and Development
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