Read by QxMD icon Read

FEMS Microbiology Ecology

Stephen Knobloch, Ragnar Jóhannsson, Viggó Marteinsson
Marine sponges can harbour diverse bacteria that contribute to host metabolism and defence. Identifying these stable members of sponge bacterial communities remains a necessary step in understanding their ecological roles and underlying co-evolutionary processes. In this study, we applied high-throughput sequencing of 16S rRNA gene amplicons, ribosomal nucleotide variant analysis and fluorescence in situ hybridisation to characterise the core members of the bacterial community in the marine sponge Halichondria panicea from Icelandic waters...
November 10, 2018: FEMS Microbiology Ecology
Vanesa Natalin Rocha Martin, Clarissa Schwab, Lukasz Krych, Evelyn Voney, Annelies Geirnaert, Christian Braegger, Christophe Lacroix
Establishment of the infant gut microbiota affects gut maturation and influences long-term health. Cutibacterium (formerly Propionibacterium) have been identified as early colonizers, but little is known about their function. Using a cultivation-dependent and -independent approach, we determined Cutibacterium prevalence, diversity and functional potential. In feces from a Swiss infant cohort (n = 38), prevalence of Propionibacterium/Cutibacterium decreased from 84% at 2 weeks, to 65% at 4 weeks, 47% at 8 weeks and 41% at 12 weeks of age...
November 1, 2018: FEMS Microbiology Ecology
Amy Fisher, Daniel Wangpraseurt, Anthony W D Larkum, Michael Johnson, Michael Kuhl, Min Chen, Hon Lun Wong, Brendan P Burns
Microbial mats and stromatolites are widespread in Hamelin Pool, Shark Bay, however the phototrophic capacity of these systems is unknown. This study has determined the optical properties and light-harvesting potential of these mats with light microsensors. These characteristics were linked via a combination of 16S rDNA sequencing, pigment analyses, and hyperspectral imaging. Local scalar irradiance was elevated over the incident down-welling irradiance by 1.5-fold, suggesting light trapping and strong scattering by the mats...
October 31, 2018: FEMS Microbiology Ecology
Arohi Srivastava, Sharad Mohan, Tim H Mauchline, Keith G Davies
Pasteuria spp. belong to a group of genetically diverse endospore-forming bacteria (phylum: Firmicutes) that are known to parasitize plant-parasitic nematodes and water fleas (Daphnia spp.). Collagen-like fibres form the nap on the surface of endospores and the genes encoding these sequences have been hypothesised to be involved in the adhesion of the endospores of Pasteuria spp. to their hosts. We report a group of seventeen unique collagen-like genes putatively encoded by Pasteuria penetrans (strain: Res148) that formed five different five different phylogenetic clusters and suggest that collagen-like proteins are an important source of genetic diversity in animal pathogenic Firmicutes including Pasteuria...
October 30, 2018: FEMS Microbiology Ecology
Thi Phi Oanh Nguyen, Martin Asser Hansen, Lars Hestbjerg Hansen, Benjamin Horemans, Soren Johannes Sorensen, René De Mot, Dirk Springael
The tfd genes mediating degradation of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) differ in composition and organization in bacterial isolates from different geographical origin and are carried by different types of mobile genetic elements (MGE). It is not known whether such global diversity of 2,4-D-catabolic MGE and their tfd gene cargo is reflected in the diversity at field scale. The genomic context of 2,4-D catabolic genes of 2,4-D-degrading isolates from two rice fields with a 2,4-D application history, located in distant provinces of the Vietnam Mekong delta, was compared...
October 30, 2018: FEMS Microbiology Ecology
Anna Voulgari-Kokota, Gudrun Grimmer, Ingolf Steffan-Dewenter, Alexander Keller
Studies on honeybees have revealed bacterial taxa which adopt key functions in the hive, in terms of nutrient uptake and immune responses. Despite solitary bees providing invaluable ecological services, the contribution of their microbial communities to larval health and the development and fitness of adults is mainly unknown. To address this gap, we conducted a 16S rDNA meta-barcoding study including larvae and stored pollen in nest chambers from two different megachilid solitary bee genera. We tested how host taxonomy, environmental context and the developmental stage of larvae determined richness and composition of associated bacterial communities...
October 27, 2018: FEMS Microbiology Ecology
Hyunjun Cho, Binu M Tripathi, Itumeleng Moroenyane, Koichi Takahashi, Dorsaf Kerfahi, Ke Dong, Jonathan M Adams
There is considerable interest in the factors which may explain variation in microbial community assembly processes. In this study, we investigated bacterial community assembly, phylogenetic diversity and the relative role of deterministic and stochastic processes along environmental gradients on Mt. Norikura, Japan. DNA extracted from soil samples collected at a range of elevations was PCR amplified for bacterial 16S rRNA gene targeting the V3-V4 region, and sequenced using Illumina MiSeq. We hypothesized that elevation would be a strong predictor of phylogenetic community assembly, with communities being more phylogenetically clustered towards higher elevations, due to more extreme physiological conditions...
October 26, 2018: FEMS Microbiology Ecology
Ephantus J Muturi, Christopher Dunlap, Jose L Ramirez, Alejandro P Rooney, Chang-Hyun Kim
Gut microbial communities of mosquitoes can influence vector susceptibility to pathogens, yet the factors that govern their composition remain poorly understood. We investigated the impact of host blood meal source on gut microbiota of Aedes aegypti L. Adult mosquitoes were fed on human, rabbit or chicken blood and their gut microbiota compared to those of sugar-fed and newly emerged adults. Microbial diversity was significantly reduced in blood-fed and sugar-fed mosquitoes but was restored to the levels of newly emerged adults' 7-days post blood meal...
October 24, 2018: FEMS Microbiology Ecology
Michael McTee, Lorinda Bullington, Matthias C Rillig, Philip W Ramsey
Many experiments that measure the response of microbial communities to heavy metals increase metal concentrations abruptly in the soil. However, it is unclear whether abrupt additions mimic the gradual and often long-term accumulation of these metals in the environment where microbial populations may adapt. In a greenhouse experiment that lasted 26 months, we tested whether bacterial communities and soil respiration differed between soils that received an abrupt or a gradual addition of copper or no copper at all...
October 23, 2018: FEMS Microbiology Ecology
Xiaoli Shi, Shengnan Li, Fan Fan, Min Zhang, Zhen Yang, Yunfeng Yang
Lake Poyang, which is connected to the Yangtze River, is the largest freshwater lake in China and experiences large and frequent changes in water levels. The seasonal diversity and composition of photosynthetic picoeukaryotes (PPEs) in Lake Poyang were investigated from flow-cytometry-sorted samples using MiSeq high-throughput sequencing. Flow cytometric counting indicated that PPEs accounted for 97% of the total picophytoplankton abundance in spring, reaching a maximum value of 6.30 × 104 cells·mL-1. PPEs in Lake Poyang showed lower diversity than those in other investigated lakes in the lower reaches of the Yangtze River and were dominated by one OTU (66...
October 22, 2018: FEMS Microbiology Ecology
Siyan Zhao, Jianzhong He
Chloroethenes are common groundwater and soil contaminants due to extensive historic utilization and inappropriate discharge. The tendency for chloroethenes to become sequestered as dense nonaqueous phase liquids (DNAPL)-a point source to groundwater contamination and causing high concentrations of chloroethenes in proximal aquifers poses a great challenge for remediation of chloroethenes contamination sites. In this study, we report isolation and characterization of a Dehalococcoides mccartyi strain 11 G which couples growth with reductive dechlorination of trichloroethenes (TCE), dichloroethene (DCE) isomers and vinyl chloride (VC) to ethene at a growth yield ranging from 2...
October 18, 2018: FEMS Microbiology Ecology
Alberto J Martín-Rodríguez, Adrián Suárez-Mesa, Fernando Artiles-Campelo, Ute Römling, Mariano Hernández
The genus Shewanella is rapidly expanding, with new species being discovered frequently. Four species have been identified as pathogenic to humans, with Shewanella algae being most relevant. Evaluation of the clinical significance of species still suffers from the imprecision of species identification. In addition, the origin of S. algae strains causing disease is unclear. To shed light upon these questions we re-identified reportedly S. algae isolates on the species level based on partial sequences of the 16S rRNA and gyrB genes in combination with multilocus sequence analysis of six housekeeping loci...
October 18, 2018: FEMS Microbiology Ecology
Maarten Van Geel, Kang Yu, Tobias Ceulemans, Gerrit Peeters, Kasper van Acker, Willem Geerts, Miguel M Ramos, Cindy Serafim, Pierre Kastendeuch, Georges Najjar, Thierry Ameglio, Jérôme Ngao, Marc Saudreau, Michael Waud, Bart Lievens, Paula M L Castro, Ben Somers, Olivier Honnay
Trees in urban areas face harsh environmental conditions. Ectomycorrhizal fungi (EcM) form a symbiosis with many tree species and provide a range of benefits to their host through their extraradical hyphal network. Although our understanding of the environmental drivers and large scale geographical variation of EcM communities in natural ecosystems is growing, our knowledge of EcM communities within and across urban areas is still limited. Here we characterized EcM communities using Illumina miseq sequencing on 175 root samples of the urban tree Tilia tomentosa from three European cities, namely Leuven (Belgium), Strasbourg (France) and Porto (Portugal)...
October 12, 2018: FEMS Microbiology Ecology
Diana Navrátilová, Petra Tláskalová, Petr Kohout, Pavel Drevojan, Karel Fajmon, Milan Chytrý, Petr Baldrian
Microbial communities in roots and shoots of plants and in soil are important for plant growth and health and take part in important ecosystem processes. Therefore, understanding the factors that affect their diversity is important. We have analysed fungal and bacterial communities associated with plant shoots, roots and soil over a 1 km2 area in a semi-natural temperate grassland with 1-43 plant species per 0.1 m2, to describe the relationships between plant and microbial diversity and to identify the drivers of bacterial and fungal community composition...
October 12, 2018: FEMS Microbiology Ecology
E Bona, N Massa, G Novello, L Boatti, P Cesaro, V Todeschini, V Magnelli, M Manfredi, E Marengo, F Mignone, G Berta, G Lingua, E Gamalero
The rhizosphere is a hot spot of microbial activity where the release of root exudates stimulates bacterial density and diversity. The majority of the bacterial cells in soil results to be viable, unculturable, but active. Proteomic tools could be useful to gain information about microbial community activity and to better understand the real interactions pathways between roots and soil. The aim of this work was to characterize the bacterial community associated with Vitis vinifera cv. Pinot Noir roots using a metaproteome approach...
October 11, 2018: FEMS Microbiology Ecology
Yang Song, Nilusha Malmuthuge, Fuyong Li, Le Luo Guan
This study evaluated the effect of feeding non-heated and heated colostrum on the mucosa- and digesta-associated microbiota in the colon of dairy calves during the first 12 h of life. Thirty-two neonatal Holstein male calves were fed: no colostrum (NC, n=8), non-heated colostrum (FC, n=12), and heated colostrum (HC (60 °C, 60 min), n=12) immediately after birth. The abundances of mucosa- and digesta-associated total bacteria were higher in the colon of FC fed calves compared to those fed no colostrum (NC) at 12 h of life...
October 11, 2018: FEMS Microbiology Ecology
Yuki Saito, Tadashi Sato, Koji Nomoto, Hirokazu Tsuji
No abstract text is available yet for this article.
October 11, 2018: FEMS Microbiology Ecology
Martin Sperfeld, Gabriele Diekert, Sandra Studenik
Sulfuritalea hydrogenivorans sk43H is well-recognized as a chemolithoautotrophic microorganism that oxidizes thiosulfate, sulfur or hydrogen. In this study, pathways for aromatic compound degradation were identified in the respective genome and proved for functionality by cultivation. S. hydrogenivorans sk43H harbors gene clusters encoding pathways for the anaerobic degradation of benzoate and phenylacetate via benzoyl-CoA as well as a partial pathway for anaerobic cinnamate degradation. Aerobic hybrid pathways were identified for the degradation of benzoate and 2-aminobenzoate...
October 10, 2018: FEMS Microbiology Ecology
Nora M Kandler, Muhammad A Abdul Wahab, Sam H C Noonan, James J Bell, Simon K Davy, Nicole S Webster, Heidi M Luter
Climate change is causing rapid changes in reef structure, biodiversity, and function, though most sponges are predicted to tolerate conditions projected for 2100. Sponges maintain intimate relationships with microbial symbionts, with previous studies suggesting that microbial flexibility may be pivotal to success under ocean acidification. We performed a reciprocal transplantation of the coral reef sponges Coelocarteria singaporensis and Stylissa cf. flabelliformis between a control reef site and an adjacent CO2 vent site in Papua New Guinea to explore how the sponge microbiome responds to ocean acidification...
October 10, 2018: FEMS Microbiology Ecology
Martin Sperfeld, Gabriele Diekert, Sandra Studenik
In this study, we established the nitrate-reducing, aromatic compound-degrading enrichment culture pMB18. Its community structure was controlled by the aromatic substrate applied: In the presence of a p-alkylated substrate, microorganisms related to Sulfuritalea, Ignavibacterium, and Comamonadaceae were abundant. Non-p-alkylated structural analogues promoted the enrichment of Azoarcus, which was probably favored by the excretion of nitrite. The analysis of the bamA gene, which is a functional marker for anaerobic aromatic compound degradation, as well as a differential abundance analysis suggested the involvement of Sulfuritalea and Comamonadaceae in the degradation of p-alkylated substrates...
October 10, 2018: FEMS Microbiology Ecology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"