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Mycotoxin Research

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https://www.readbyqxmd.com/read/28730564/a-new-approach-to-assess-occupational-exposure-to-airborne-fungal-contamination-and-mycotoxins-of-forklift-drivers-in-waste-sorting-facilities
#1
Carla Viegas, Tiago Faria, Ana Cebola de Oliveira, Liliana Aranha Caetano, Elisabete Carolino, Anita Quintal-Gomes, Magdalena Twarużek, Robert Kosicki, Ewelina Soszczyńska, Susana Viegas
The waste management industry is an important employer, and exposure of waste-handling workers to microorganisms is considered an occupational health problem. Besides fungal contamination, it is important to consider the co-occurrence of mycotoxins in this setting. Forklifts with closed cabinet and air conditioner are commonly used in waste industry to transport waste and other products within the facilities, possibly increasing the risk of exposure under certain conditions. The aim of this study was to assess the fungal contamination and mycotoxin levels in filters from the air conditioning system of forklift cabinets, as an indicator to assess occupational exposure of the drivers working in a waste sorting facility...
July 20, 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/28687999/use-of-yeast-pichia-kudriavzevii-as-a-novel-feed-additive-to-ameliorate-the-effects-of-aflatoxin-b1-on-broiler-chicken-performance
#2
A P Magnoli, M C Rodriguez, M L González Pereyra, V L Poloni, M F Peralta, A J Nilson, R D Miazzo, G Bagnis, S M Chiacchiera, L R Cavaglieri
The aim of this study was to evaluate the efficacy of autochthonous Pichia kudriavzevii as a novel bioadsorbent for aflatoxin B1 (AFB1). The selection of this yeast was based on the AFB1 adsorption capacity previously demonstrated in vitro (Magnoli et al. 2016). One-day-old Cobb broilers (n = 160) were randomly assigned to four dietary treatments (T1: basal diet (B); T2: B + 0.1% yeast; T3: B + AFB1, 100 μg/kg; T4: B + 0.1% yeast + AFB1, 100 μg/kg). Performance parameters (average daily weight gain body, average daily consumption, feed conversion ratio, carcass weight, and dead weight), biochemical parameters (albumin, globulin, and albumin/globulin), liver pathological changes, and AFB1 residual levels in the liver and excreta were evaluated...
July 8, 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/28687998/effects-of-low-to-moderate-levels-of-deoxynivalenol-on-feed-and-water-intake-weight-gain-and-slaughtering-traits-of-broiler-chickens
#3
A Lucke, B Doupovec, P Paulsen, Q Zebeli, J Böhm
The aim of the study was to evaluate the effects of low to moderate oral exposure to the Fusarium toxin deoxynivalenol (DON; derived from culture material) on performance, water intake, and carcass parameters of broilers during early and late developmental phases. A total of 160 Ross 308 broilers were randomly allocated to four different feeding groups (n = 40/group) including 0 (control), 2.5, 5, and 10 mg DON/kg wheat-soybean meal-based feed. Three consecutive replicates of the experiment were performed...
July 7, 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/28612272/geographic-distribution-of-phylogenetic-species-of-the-fusarium-graminearum-species-complex-and-their-8-ketotrichothecene-chemotypes-on-wheat-spikes-in-iran
#4
Mostafa Abedi-Tizaki, Doustmorad Zafari
Isolates of the Fusarium graminearum species complex (FGSC, n = 446) were collected from wheat spikes from northern and western regions of Iran with a history of Fusarium head blight (FHB) occurrences. The trichothecene mycotoxin genotypes/chemotypes, the associated phylogenetic species, and geographical distribution of these isolates were analyzed. Two phylogenetic species, Fusarium asiaticum and F. graminearum, were identified and were found to belong to sequence characterized amplified region (SCAR) groups V and I...
August 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/28597241/biocontrol-of-the-toxigenic-plant-pathogen-fusarium-culmorum-by-soil-fauna-in-an-agroecosystem
#5
Friederike Meyer-Wolfarth, Stefan Schrader, Elisabeth Oldenburg, Joachim Weinert, Joachim Brunotte
In 2011 and 2013, a field experiment was conducted in a winter wheat field at Adenstedt (northern Germany) to investigate biocontrol and interaction effects of important members of the soil food web (Lumbricus terrestris, Annelida; Folsomia candida, Collembola and Aphelenchoides saprophilus, Nematoda) on the phytopathogenic fungus Fusarium culmorum in wheat straw. Therefore, soil fauna was introduced in mesocosms in defined numbers and combinations and exposed to either Fusarium-infected or non-infected wheat straw...
August 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/28573418/rising-atmospheric-co2-concentration-may-imply-higher-risk-of-fusarium-mycotoxin-contamination-of-wheat-grains
#6
Szilvia Bencze, Katalin Puskás, Gyula Vida, Ildikó Karsai, Krisztina Balla, Judit Komáromi, Ottó Veisz
Increasing atmospheric CO2 concentration not only has a direct impact on plants but also affects plant-pathogen interactions. Due to economic and health-related problems, special concern was given thus in the present work to the effect of elevated CO2 (750 μmol mol(-1)) level on the Fusarium culmorum infection and mycotoxin contamination of wheat. Despite the fact that disease severity was found to be not or little affected by elevated CO2 in most varieties, as the spread of Fusarium increased only in one variety, spike grain number and/or grain weight decreased significantly at elevated CO2 in all the varieties, indicating that Fusarium infection generally had a more dramatic impact on the grain yield at elevated CO2 than at the ambient level...
August 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/28488100/mycotoxins-in-organic-and-conventional-cereals-and-cereal-products-grown-and-marketed-in-croatia
#7
Jelka Pleadin, Mladenka Malenica Staver, Ksenija Markov, Jadranka Frece, Manuela Zadravec, Vesna Jaki, Igor Krupić, Nada Vahčić
In this study, the levels of aflatoxin B1 (AFB1), ochratoxin A (OTA), zearalenone (ZEN), deoxynivalenol (DON) and fumonisins (FUM) in unprocessed cereals (n = 189) and cereal-based products (n = 61) were determined using validated ELISA methods. All samples originated from either conventional or organic production corresponded to the 2015 harvest in Croatia. Based on the mean mycotoxin concentrations, the risk for the consumer to exceed the tolerable daily intake (TDI) for these toxins by the consumption of both types of cereals and cereal-based products was assessed...
August 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/28474303/chronic-don-exposure-and-acute-lps-challenge-effects-on-porcine-liver-morphology-and-function
#8
Lydia Renner, Stefan Kahlert, Tanja Tesch, Erik Bannert, Jana Frahm, Anikó Barta-Böszörményi, Jeannette Kluess, Susanne Kersten, Peter Schönfeld, Hermann-Josef Rothkötter, Sven Dänicke
The aim of the present study was to examine the role of chronic deoxynivalenol (DON) exposition on the liver morphology and function in combination with pre- and post-hepatic lipopolysaccharide (LPS) stress in young pigs fed for 4 weeks with a DON-contaminated diet (4.59 mg/kg feed). At the end of the experiment, LPS (7.5 μg/kg BW) was administered for 1 h pre-hepatically (Vena portae hepatis) or post-hepatically (Vena jugularis). Liver morphology was macroscopically checked and showed haemorrhage in all LPS groups, significantly higher relative liver weights, accompanied by marked oedema in the gallbladder wall...
August 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/28470577/plasma-kinetics-and-matrix-residues-of-deoxynivalenol-don-and-zearalenone-zen-are-altered-in-endotoxaemic-pigs-independent-of-lps-entry-site
#9
Erik Bannert, Tanja Tesch, Jeannette Kluess, Hana Valenta, Jana Frahm, Susanne Kersten, Stefan Kahlert, Lydia Renner, Hermann-Josef Rothkötter, Sven Dänicke
This study aimed to investigate a potential modulatory effect of E. coli lipopolysaccharide (LPS) on the kinetics of deoxynivalenol (DON) and zearalenone (ZEN) after pre- or post-hepatic LPS administration to unravel the putative role of the liver. Fifteen barrows were fed a diet containing mycotoxin-contaminated maize (4.59 mg DON/kg feed, 0.22 mg ZEN/kg feed) for 29 days and equipped with pre-hepatic catheters (portal vein, "po") and post-hepatic catheters (jugular vein, "ju"), facilitating simultaneous infusion of LPS ("LPS group", 7...
August 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/28455556/fusarium-diseases-of-maize-associated-with-mycotoxin-contamination-of-agricultural-products-intended-to-be-used-for-food-and-feed
#10
REVIEW
Elisabeth Oldenburg, Frank Höppner, Frank Ellner, Joachim Weinert
Infections of maize with phytopathogenic and toxinogenic Fusarium spp. may occur throughout the cultivation period. This can cause different types of diseases in vegetative and generative organs of the plant. Along with these infections, mycotoxins are often produced and accumulated in affected tissues, which could pose a significant risk on human and animal health when entering the food and feed chain. Most important fungal species infecting European maize belong to the Fusarium sections Discolour and Liseola, the first being more prevalent in cooler and humid climate regions than the second predominating in warmer and dryer areas...
August 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/28434149/solvent-dependent-transformation-of-aflatoxin-b1-in-soil
#11
James M Starr, Blake R Rushing, Mustafa I Selim
To date, all studies of aflatoxin B1 (AFB1) transformation in soil or in purified mineral systems have identified aflatoxins B2 (AFB2) and G2 (AFG2) as the primary transformation products. However, identification in these studies was made using thin layer chromatography which has relatively low resolution, and these studies did not identify a viable mechanism by which such transformations would occur. Further, the use of methanol as the solvent delivery vehicle in these studies may have contributed to formation of artifactual transformation products...
August 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/28382601/pre-harvest-aflatoxins-and-aspergillus-flavus-contamination-in-variable-germplasms-of-red-chillies-from-kunri-pakistan
#12
Shaista Akhund, Abida Akram, Nafeesa Qudsia Hanif, Rahmatullah Qureshi, Farah Naz, Brian Gagosh Nayyar
Various cultivars of red chilli were collected from a small town named Kunri, located in the province Sindh, Pakistan. This town is a hub of red chilli production in Asia. A total of 69 samples belonging to 6 cultivars were obtained and analysed for the occurrence of aflatoxins and Aspergillus flavus, to explore the potential of resistant and susceptible germplasm. Aflatoxins were detected by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC), while A. flavus was isolated and identified using agar plate, blotter paper, deep freezing and dilution techniques...
May 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/28378232/detection-of-cyclopiazonic-acid-cpa-in-maize-by-immunoassay
#13
C M Maragos, K K Sieve, J Bobell
Cyclopiazonic acid (α-CPA) is a tremorgenic mycotoxin that is commonly produced by certain species of the aspergilli, in particular Aspergillus flavus, which is more widely known for production of the aflatoxins. Despite the fact that α-CPA may co-occur with aflatoxins, immunoassay-based methods for monitoring for CPA have not been widely developed. We report the development and evaluation of several monoclonal antibodies (mAbs) for α-CPA. Two mAbs in particular were very sensitive, with IC50s of 1.1 and 1 ng/mL (clones 1418 and 1231, respectively)...
May 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/28281009/verrucarin-a-and-roridin-e-produced-on-spinach-by-myrothecium-verrucaria-under-different-temperatures-and-co2-levels
#14
Ilenia Siciliano, Pietro Bosio, Giovanna Gilardi, Maria Lodovica Gullino, Angelo Garibaldi
The behavior of Myrothecium verrucaria, artificially inoculated on spinach, was studied under seven different temperature conditions (from 5 to 35 °C) and under eight different combinations of temperature and CO2 concentration (14-30 °C and 775-870 or 1550-1650 mg/m(3)). The isolate used for this study was growing well on spinach, and the mycotoxins verrucarin A and roridin E were produced under all tested temperature and CO2 conditions. The maximum levels of verrucarin A (18.59 ng/g) and roridin E (49...
May 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/28265970/fusarium-verticillioides-and-fumonisin-contamination-in-bt-and-non-bt-maize-cultivated-in-brazil
#15
COMPARATIVE STUDY
Vinícius M Barroso, Liliana O Rocha, Tatiana A Reis, Gabriela M Reis, Aildson P Duarte, Marcos D Michelotto, Benedito Correa
Fusarium verticillioides is one of the main pathogens of maize, causing ear and stalk rots. This fungus is also able to produce high levels of fumonisins, which have been linked to various illnesses in humans and animals. Previous studies have shown that maize hybrids genetically modified with the cry genes from the bacterium Bacillus thuringiensis (Bt) presented lower incidence of F. verticillioides and fumonisin levels, presumably through the reduction of insects, which could act as vectors of fungi. The aim of this study was to assess the incidence of F...
May 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/28247323/aflatoxins-discolouration-and-insect-damage-in-dried-cowpea-and-pigeon-pea-in-malawi-and-the-effectiveness-of-flotation-washing-operation-in-eliminating-the-aflatoxins
#16
Limbikani Matumba, Lazarus Singano, Lawrent Pungulani, Naomi Mvula, Annie Matumba, Charles Singano, Grey Matita
Aflatoxin contamination and biodeterioration were examined in 302 samples of dry cowpeas and pigeon peas that were randomly purchased from 9 districts of the Southern Region of Malawi during July and November 2015. Further, the impact of flotation/washing on aflatoxin levels on the pulses was elucidated. Aflatoxin analyses involved immunoaffinity column (IAC) clean-up and HPLC quantification with fluorescence detection (FLD) while legume biodeterioration assessments were done by visual inspection. Aflatoxins were frequently detected in cowpea (24%, max...
May 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/28124218/aflatoxin-b1-levels-in-groundnut-products-from-local-markets-in-zambia
#17
Samuel M C Njoroge, Limbikani Matumba, Kennedy Kanenga, Moses Siambi, Farid Waliyar, Joseph Maruwo, Norah Machinjiri, Emmanuel S Monyo
In Zambia, groundnut products (milled groundnut powder, groundnut kernels) are mostly sold in under-regulated markets. Coupled with the lack of quality enforcement in such markets, consumers may be at risk to aflatoxin exposure. However, the level of aflatoxin contamination in these products is not known. Compared to groundnut kernels, milled groundnut powder obscures visual indicators of aflatoxin contamination in groundnuts such as moldiness, discoloration, insect damage or kernel damage. A survey was therefore conducted from 2012 to 2014, to estimate and compare aflatoxin levels in these products (n = 202), purchased from markets in important groundnut growing districts and in urban areas...
May 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/28083735/toxicological-effects-of-aflatoxin-b1-on-the-earthworm-eisenia-fetida-as-determined-in-a-contact-paper-test
#18
J Szabó-Fodor, I Bors, G Nagy, M Kovács
In this study, aflatoxin B1 (AFB1) toxicity toward the earthworm Eisenia fetida (Savigny 1826) was evaluated in contact paper test systems containing distilled water and ethanol or 20 to 400 μg/ml of AFB1 over 72 h of exposure. The results indicated that AFB1 could induce significant damage to earthworms (coiling, curling, excessive mucus secretion, clitellum swelling) at greater than 75 μg/ml. Moreover, AFB1 had harmful effects on E. fetida (degenerative changes such as bulging of the clitella regions) at levels higher than 150 μg/ml...
May 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/28035652/determination-of-serum-aflatoxin-b1-lysine-to-evaluate-the-efficacy-of-an-aflatoxin-adsorbing-feed-additive-in-pigs-fed-an-aflatoxin-b1-contaminated-diet
#19
Mayra Carraro Di Gregorio, Alessandra Vincenzi Jager, Pollyana Cristina Maggio Castro Souto, Aline Alves Costa, George Edwin Rottinghaus, Danielle Passarelli, Fabio Enrique Lemos Budiño, Carlos Humberto Corassin, Carlos Augusto Fernandes Oliveira
In this study, serum aflatoxin B1 (AFB1)-lysine was determined in order to evaluate the in vivo efficacy of a hydrated sodium calcium aluminosilicate (HSCAS) in pigs fed AFB1. Twenty-four 49-day-old crossbred barrows were maintained in individual cages and allowed ad libitum access to feed and water. A completely randomized design was used with six animals assigned to each of four dietary treatments for 21 days as follows: (A) basal diet (BD), (B) BD supplemented with 0.5 % HSCAS, (C) BD supplemented with 1...
May 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/28032266/aflatoxin-m1-in-human-breast-milk-in-southeastern-turkey
#20
Serap Kılıç Altun, Semra Gürbüz, Emin Ayağ
This study was performed to determine aflatoxin M1 (AFM1) in human breast milk samples collected in Şanlıurfa, located in Southeastern region of Turkey, and to investigate a possible correlation between AFM1 occurrence (frequency and levels) and sampling seasons. Human breast milk samples collected in December 2014 and in June 2015 from a total of 74 nursing women, both outpatient and inpatient volunteers in hospitals located in Şanlıurfa, Turkey, were analyzed using competitive enzyme-linked immunosorbent assay (ELISA) for the presence of AFM1...
May 2017: Mycotoxin Research
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