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Cytotechnology

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https://www.readbyqxmd.com/read/27905026/native-matrix-based-human-lung-alveolar-tissue-model-in-vitro-studies-of-the-reparatory-actions-of-mesenchymal-stem-cells
#1
Ieva Bruzauskaite, Jovile Raudoniute, Jaroslav Denkovskij, Edvardas Bagdonas, Sandra Meidute-Abaraviciene, Vaida Simonyte, Daiva Bironaite, Almantas Siaurys, Eiva Bernotiene, Ruta Aldonyte
Studies of lung diseases in vitro often rely on flat, plastic-based monocultures, due to short lifespan of primary cells, complicated anatomy, lack of explants, etc. We hereby present a native 3D model with cues for repopulating epithelial cells. Abilities of mesenchymal stem cells (MSC) to modulate bacterial lipopolysaccharide (LPS) and cigarette smoke-induced injury to pulmonary epithelium were tested in our model. Post-mortem human lung tissue was sliced, cut and decellularized. Resulting matrix pads were reseeded with pulmonary epithelium (A549 line)...
December 1, 2016: Cytotechnology
https://www.readbyqxmd.com/read/27905025/flavonoids-from-heliotropium-subulatum-exudate-and-their-evaluation-for-antioxidant-antineoplastic-and-cytotoxic-activities-ii
#2
Bharat Singh, Pooran M Sahu, Ram A Sharma
The flavonoids are the largest group of phenolic compounds isolated from a wide range of higher plants. These compounds work as antimicrobials, anti-insect agents and protect plants from other types of biotic and abiotic stresses. Various researchers have suggested that flavonoids possessed antioxidant, antineoplastic and cytotoxic activities. The main objective of this study was to test dichloromethane fraction of resinous exudate of Heliotropium subulatum for their antioxidant, antineoplastic and cytotoxic activities, as well as to search new antioxidant and antineoplastic agents for pharmaceutical formulations...
November 30, 2016: Cytotechnology
https://www.readbyqxmd.com/read/27905024/potential-anti-genotoxic-effect-of-sodium-butyrate-to-modulate-induction-of-dna-damage-by-tamoxifen-citrate-in-rat-bone-marrow-cells
#3
Haidan M El-Shorbagy
Sodium butyrate (SB) is one of the histone deacetylase inhibitors (HDACi's) that is recently evidenced to have a prooxidant activity and an ability to reduce hydrogen peroxide-induced DNA damage. Since the majority of estrogen receptor positive breast cancer patients are treated with tamoxifen citrate (TC), which exerts well established oxidative and genotoxic effects, thus the basic objective of this study is to determine whether SB could ameliorate or curate tamoxifen citrate-induced oxidative DNA damage and genotoxic effect in vivo through up-regulation of some antioxidant enzymes...
November 30, 2016: Cytotechnology
https://www.readbyqxmd.com/read/27900626/selection-of-chemically-defined-media-for-cho-cell-fed-batch-culture-processes
#4
Xiao Pan, Mathieu Streefland, Ciska Dalm, René H Wijffels, Dirk E Martens
Two CHO cell clones derived from the same parental CHO(BC®) cell line and producing the same monoclonal antibody (BC-G, a low producing clone; BC-P, a high producing clone) were tested in four basal media in all possible combinations with three feeds (=12 conditions) in fed-batch cultures. Higher amino acid feeding did not always lead to higher mAb production. The two clones showed differences in cell physiology, metabolism and optimal medium-feed combinations. During the phase transitions of all cultures, cell metabolism showed a shift represented by lower specific consumption and production rates, except for the specific glucose consumption rate in cultures fed by Actifeed A/B...
November 29, 2016: Cytotechnology
https://www.readbyqxmd.com/read/27896559/antiviral-activity-of-hemolymph-of-podalia-against-rubella-virus
#5
N D Carvalho, R Z Mendonça, M I Oliveira, S P Curti, T F Barbosa, P E Silva, N N Taniwaki, M Tonelotto, D N S Giovanni, R H P Moraes, C A Figueiredo
Many active principles produced by animals, plants and microorganisms have been employed in the development of new drugs for the treatment of human diseases. Among animals known to produce pharmacologically active molecules that interfere in human cell physiology. Rubella virus (genus Rubivirus, family Togaviridae) is a single stranded RNA virus of positive genome polarity. Rubella virus infection of susceptible women during the first trimester of pregnancy often results in long-term virus persistence in the fetus causing multiple organ abnormalities...
November 28, 2016: Cytotechnology
https://www.readbyqxmd.com/read/27896558/h3k9ac-involved-in-the-decondensation-of-spermatozoal-nuclei-during-spermatogenesis-in-chinese-mitten-crab-eriocheir-sinensis
#6
Genliang Li, Xianjiang Kang, Shumei Mu, Mingshen Guo, Shiwen Huang, Qinna Chen, Song Nong, Xiaomin Huang, Hongliu Hu, Ke Sun
As a well-known crustacean model species, the Chinese mitten crab Eriocheir sinensis presents spermatozoa with decondensed DNA. Our aim was to analyze structural distribution of the histone H3 and its acetylated lysine 9 (H3K9ac) during spermatogenesis for the mechanistic understanding of the nuclear decondensation of the spermatozoa in E. sinensis. Using specific antibodies, we followed the structural distribution and acetylated lysine 9 of the histone H3 during spermatogenesis, especially spermiogenesis, of E...
November 28, 2016: Cytotechnology
https://www.readbyqxmd.com/read/27896557/cytotoxic-and-apoptotic-activities-of-extract-of-amaranthus-spinosus-l-in-allium-cepa-and-human-erythrocytes
#7
V Prajitha, J E Thoppil
The present study examined the apoptosis inducing effects of Amaranthus spinosus L. aqueous extract in Allium cepa root meristematic cells and human erythrocytes. Cytogenetic assay revealed many apoptosis inducing cytogenetic aberrations viz., cytoplasmic breakage, cytoplasmic disintegration, cytoplasmic shrinkage, receding of cytoplasm, cytoplasmic vacuolation, enucleated cell, ghost cell, nuclear vacuolation, nuclear fragmentation and nuclear disintegration. A remarkable modification of red blood cell surface morphology was observed in the result of RBC assay...
November 28, 2016: Cytotechnology
https://www.readbyqxmd.com/read/27896556/towards-standardized-automated-immunomonitoring-an-automated-elispot-assay-for-safe-and-parallelized-functionality-analysis-of-immune-cells
#8
J C Neubauer, I Sébastien, A Germann, S C Müller, A Meyerhans, H von Briesen, H Zimmermann
The ELISpot assay is used for the detection of T cell responses in clinical trials and vaccine evaluations. Standardization and reproducibility are necessary to compare the results worldwide, inter- and intra-assay variability being critical factors. To assure operator safety as well as high-quality experiment performance, the ELISpot assay was implemented on an automated liquid handling platform, a Tecan Freedom EVO. After validation of the liquid handling, automated loading of plates with cells and reagents was investigated...
November 28, 2016: Cytotechnology
https://www.readbyqxmd.com/read/27761772/toxicity-of-antimony-copper-cobalt-manganese-titanium-and-zinc-oxide-nanoparticles-for-the-alveolar-and-intestinal-epithelial-barrier-cells-in-vitro
#9
T Titma, R Shimmo, J Siigur, A Kahru
Heavy metals are found naturally on Earth and exposure to them in the living environment is increasing as a consequence of human activity. The toxicity of six different metal oxide nanoparticles (NP) at different points in time was compared using resazurin assay. After incubating Caco2 and A549 cells with 100 μg/mL of Sb2O3, Mn3O4 and TiO2 nanoparticles (NPs) for 24 h no toxic effects were observed while Co3O4 and ZnO NPs had moderate effects and CuO NPs were toxic below 100 μg/mL (24 h EC25 = 11 for A549 and 71 μg/mL for Caco2)...
December 2016: Cytotechnology
https://www.readbyqxmd.com/read/27761771/cryoprotection-synergism-between-glycerol-and-dimethyl-sulfoxide-improves-the-mitochondrial-transmembrane-potential-plasmalemma-acrosomal-and-dna-integrities-and-in-vivo-fertility-of-water-buffalo-bubalus-bubalis-spermatozoa
#10
Syed Aftab Hussain Shah, Syed Murtaza Hassan Andrabi, Hussain Ahmed, Irfan Zia Qureshi
The objective of the study was to devise a cryoprotection synergism between glycerol and dimethyl sulfoxide (DMSO) for water buffalo spermatozoa. Additionally, the effect of best evolved concentrations of glycerol and DMSO in extender was assessed on in vivo fertility of buffalo spermatozoa. Ejaculates (n = 30) were equally distributed into five aliquots; first aliquot was diluted at 37 °C in extender having 7 % glycerol (control); second aliquot was diluted at 37 °C as well as at 4 °C in extender having 3...
December 2016: Cytotechnology
https://www.readbyqxmd.com/read/27757713/characterization-of-the-growth-modulatory-activities-of-osteoblast-conditioned-media-on-cord-blood-progenitor-cells
#11
Ahmad Abu-Khader, Roya Pasha, Gwendoline C D Ward, Gavin Boisjoli, Nicolas Pineault
Engraftment outcomes are strongly correlated with the numbers of hematopoietic stem and progenitor cells (HSPC) infused. Expansion of umbilical cord blood (CB) HSPC has gained much interest lately since infusion of expanded HSPC can accelerate engraftment and improve clinical outcomes. Many novel protocols based on different expansion strategies of HSPC and their downstream derivatives are under development. Herein, we describe the production and properties of serum-free medium (SFM) conditioned with mesenchymal stromal cells derived-osteoblasts (OCM) for the expansion of umbilical CB cells and progenitors...
December 2016: Cytotechnology
https://www.readbyqxmd.com/read/27757712/rna-interference-mediated-downregulation-of-human-telomerase-reverse-transcriptase-htert-in-ln18-cells
#12
Ch Lavanya, M K Sibin, M M Srinivas Bharath, M Jeru Manoj, Manjunatha M Venkataswamy, Dhananjaya I Bhat, K V L Narasinga Rao, G K Chetan
Human telomerase reverse transcriptase (hTERT) gene is a biomarker for the targeted therapy in various cancers. Presence of increased telomerase activity is a common feature of all cancers including glioblastoma. Both RNA and catalytic subunits of hTERT are the target sites for blocking its activity. The current study focuses on the expression of hTERT in glioblastoma and its regulation using two different novel siRNAs (small interfering RNA). Our patient data demonstrated increased expression of hTERT, which could be correlated with carcinogenesis in glioma...
December 2016: Cytotechnology
https://www.readbyqxmd.com/read/27757711/isolation-and-characterization-of-putative-mesenchymal-stem-cells-from-mammalian-gut
#13
Yulong Tao, Sang Zhu, Hong Yang, Fei Huang, Hui Fu, Xia Tao
Here, we provide a protocol for reliable isolation and subculture of putative mesenchymal stem cells from mice colons. This method provides a good approach to cultivate and characterize putative colonic mesenchymal stem cells (cMSCs). A high purity of cMSCs can be obtained according to this protocol. The whole isolation processes of cMSCs take about 2 h with two important digestion steps involved. Only with common culture medium, maturation of cMSCs in culture proceeds approximately 2 weeks to allow relevant researches to be conducted...
December 2016: Cytotechnology
https://www.readbyqxmd.com/read/27757710/the-role-of-natural-indigo-dye-in-alleviation-of-genotoxicity-of-sodium-dithionite-as-a-reducing-agent
#14
İdris Bektaş, Şengül Karaman, Emel Dıraz, Mustafa Çelik
Indigo blue is a natural dye used for thousands of years by civilizations to dye fabric blue and it is naturally obtained from Isatis tinctoria. I. tinctoria is not only used for extraction of indigo blue color but also used medicinally in Traditional Chinese Medicine because of its active compounds. Sodium dithionite (Na2S2O4) is used in dye bath for indigo blue extraction, but this reducing agent and its derivatives are major pollutants of textile industry and subsequently have hazardous influences on public health...
December 2016: Cytotechnology
https://www.readbyqxmd.com/read/27752841/antioxidant-and-antigenotoxic-potencies-of-sempervivum-armenum-on-human-lymphocytes-in-vitro
#15
Serap Sunar, Mustafa Anar, Meryem Sengul, Guleray Agar
In this research, the genotoxic and antigenotoxic effects of methanol extract of Sempervivum armenum (MSA) were studied using micronucleus (MN) test and sister chromatid exchange (SCE) test systems in cultured human peripheral blood cells. According to the SCE and MN tests results, MSA reduced the genotoxic effects of aflatoxin B1. In order to explain the reason for the antigenotoxic effects of MSA, antioxidants levels were determined. Cotreatments of 5, 10, 20 mg/mL concentrations of MSA with aflatoxin B1 decreased the frequencies of SCE, MN and the malondialdehyde level and increased the amount of superoxide dismutase, glutathione and glutathione peroxidase which were decreased by aflatoxin...
December 2016: Cytotechnology
https://www.readbyqxmd.com/read/27752840/construction-and-characterization-of-a-multilayered-gingival-keratinocyte-culture-model-the-turk-u-model
#16
Ulvi K Gursoy, Mervi Gursoy, Eija Könönen, Herman O Sintim, Veli-Jukka Uitto, Stina Syrjänen
In construction of epithelial cells as multilayers, the cells are grown submerged to confluence on fibroblast-embedded collagen gels and, then, lifted to air to promote their stratification. We recently demonstrated that gingival epithelial cells form uniform monolayers on semi-permeable nitrocellulose membranes, supported with a semi-solid growth medium, which allows the cells to grow at an air-liquid-solid interface from the beginning of the culturing protocol. In this study, the aim was to further develop our previous model to form a multilayered gingival epithelial culture model...
December 2016: Cytotechnology
https://www.readbyqxmd.com/read/27744595/simulated-microgravity-inhibits-the-migration-of-mesenchymal-stem-cells-by-remodeling-actin-cytoskeleton-and-increasing-cell-stiffness
#17
Xinjian Mao, Zhe Chen, Qing Luo, Bingyu Zhang, Guanbin Song
Exposure to microgravity during space flight affects almost all human physiological systems. Migration, proliferation, and differentiation of stem cells are crucial for tissues repair and regeneration. However, the effect of microgravity on the migration potentials of bone marrow mesenchymal stem cells (BMSCs) is unclear, which are important progenitor and supporting cells. Here, we utilized a clinostat to model simulated microgravity (SMG) and found that SMG obviously inhibited migration of rat BMSCs. We detected significant reorganization of F-actin filaments and increased Young's modulus of BMSCs after exposure to SMG...
December 2016: Cytotechnology
https://www.readbyqxmd.com/read/27709375/carbohydrates-chitosan-composite-carrier-for-vero-cell-culture
#18
Ya-Ching Lin, Guan-Ting Chen, Sheng-Chi Wu
In this study, carbohydrate-chitosan composite including glucose-chitosan, sucrose-chitosan and starch-chitosan with varied carbohydrate concentrations were prepared as carriers for Vero cell culture. Our results show that among these composites, 30 % starch-chitosan composite (STC) were the best carriers for the growth of Vero cells. The initial number of attached cells on the surface of composite carriers did not have any significant effect on subsequent cell production. A higher glucose level in the growth medium during the exponential phase of cell growth, however, played an important factor for cell production...
December 2016: Cytotechnology
https://www.readbyqxmd.com/read/27686814/in-vitro-cytotoxic-and-genotoxic-evaluation-of-peptides-used-in-nuclear-medicine-dotatate-and-ubiquicidin29-41-in-cho-k1-cells
#19
Ivette Zegarra Ocampo, Priscila de Queiroz Souza Passos, Luma Ramirez de Carvalho, Camila Ayala Lira da Cruz, Natália Mencacci Esteves-Pedro, Fabiana Medeiros da Silva, Olga Zazuco Higa, Luiz Alberto Pereira Dias, Kayo Okazaki, Daniel Perez Vieira
Micronucleus (MN) assay constitutes a valuable surrogate to the chromosome aberration technique for in vitro testing of the genotoxicity of substances. As test substances, two peptidic compounds (DOTATATE and Ubiquicidin29-41) used in nuclear medicine, were tested for in vitro cytotoxicity and genotoxicity in CHO-K1 cells. None of the compounds showed detectable cytotoxicity (0.5-7.3 ng/mL for DOTATATE and 0.3-4.5 ng/mL for UBI29-41), genotoxicity (0.72, 7.2 and 72.0 ng/ml for DOTATATE and 0.45, 4.5 and 45...
December 2016: Cytotechnology
https://www.readbyqxmd.com/read/27670438/a-scaffold-free-surface-culture-of-b16f10-murine-melanoma-cells-based-on-magnetic-levitation
#20
Yun Gyu Jeong, Jin Sil Lee, Jae Kwon Shim, Won Hur
Multicellular spheroids are obtained in a variety of three-dimensional (3D) culture systems without the use of supporting scaffold. We present here a 3D culture method that resulted in a multicellular sheet under scaffold-free conditions. A floating disk-shaped 3D culture was prepared by magnetic levitation of B16F10 cells that has ingested Fe3O4-containing fibroin microspheres. The melanoma disk grew up to 19 mm in diameter and the thickness was ranged between 80 and 100 μm. The 3D culture was filled with closely packed cells that were proliferating exponentially at a specific growth rate of µ = 0...
December 2016: Cytotechnology
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