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Cytotechnology

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https://www.readbyqxmd.com/read/28337561/optimization-of-ultraviolet-ozone-treatment-process-for-improvement-of-polycaprolactone-pcl-microcarrier-performance
#1
Nurhusna Samsudin, Yumi Zuhanis Has-Yun Hashim, Mohd Azmir Arifin, Maizirwan Mel, Hamzah Mohd Salleh, Iis Sopyan, Dzun Noraini Jimat
Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the surface functionality of certain polymeric microcarriers for effective cell attachment and growth remains a challenge. Polycaprolactone (PCL), a biodegradable polymer has received considerable attention due to its good mechanical properties and degradation rate. The drawback is the non-polar hydrocarbon moiety which makes it not readily suitable for cell attachment. This report concerns the modification of PCL microcarrier surface (introduction of functional oxygen groups) using ultraviolet irradiation and ozone (UV/O3) system and investigation of the effects of ozone concentration, the amount of PCL and exposure time; where the optimum conditions were found to be at 60,110...
March 23, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28321780/isolation-culture-and-identification-of-pulmonary-arterial-smooth-muscle-cells-from-rat-distal-pulmonary-arteries
#2
Gongyong Peng, Juan Xu, Rongmin Liu, Zhenli Fu, Shaoxing Li, Wei Hong, Jinglong Chen, Bing Li, Pixin Ran
The culture of pulmonary arterial smooth muscle cells (PASMCs) is one of the most powerful tools for exploring the mechanisms of pulmonary hypertension (PH). Both pulmonary vasoconstriction and remodeling occur predominantly in distal pulmonary arteries (PA). In this study, we provide our detailed and standardized protocol for easy isolation and culture of PASMCs from rat distal PA to supply every investigator with a simple, economical and useful method in studying PH. The protocol can be divided into four stages: isolation of distal PA, isolation of cells, growth in culture and passage of cells...
March 21, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28321779/transfection-of-bovine-fetal-fibroblast-with-polyethylenimine-pei-nanoparticles-effect-of-particle-size-and-presence-of-fetal-bovine-serum-on-transgene-delivery-and-cytotoxicity
#3
D O Forcato, A E Fili, F E Alustiza, J M Lázaro Martínez, S Bongiovanni Abel, M F Olmos Nicotra, A P Alessio, N Rodríguez, C Barbero, P Bosch
The development of efficient transfection protocols for livestock cells is crucial for implementation of cell-based transgenic methods to produce genetically modified animals. We synthetized fully deacylated linear 22, 87 and 217 kDa polyethylenimine (PEI) nanoparticles and compared their transfection efficiency and cytotoxicity to commercial branched 25 kDa PEI and linear 58 kDa poly(allylamine) hydrochloride. We studied the effect of PEI size and presence of serum on transfection efficiency on primary cultures of bovine fetal fibroblasts and established cells lines (HEK 293 and Hep G2)...
March 20, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28321778/effects-of-mir-21-downregulation-and-silibinin-treatment-in-breast-cancer-cell-lines
#4
Zohreh Jahanafrooz, Nasrin Motamed, Behnaz Bakhshandeh
Silibinin is a natural polyphenol with high antioxidant and anticancer properties, which causes cell cycle arrest and apoptosis in most cancer cell types including breast cancer, but the in-line mechanisms, are still unknown. Silibinin significantly downregulated oncomiR miR-21 expression in breast cancer cells. Here the effect of anti-miR-21 on cell viability, apoptotic induction, cell cycle distribution, and the expression levels of downstream targets of miR-21 were investigated in MCF-7 and T47D cells. MiR-21 mimic transfection was also applied in silibinin treated samples to evaluate functional role of miR-21downregulation on silibinin effects...
March 20, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28321777/digoxin-reduces-the-mutagenic-effects-of-mitomycin-c-in-human-and-rodent-cell-lines
#5
Júlia Teixeira de Oliveira, Maria C da Silva Barbosa, Luiz F de Camargos, Isabella Viana Gomes da Silva, Fernando de Pilla Varotti, Luciana M da Silva, Leonardo Marmo Moreira, Juliana Pereira Lyon, Vanessa J da Silva Vieira Dos Santos, Fabio Vieira Dos Santos
Digoxin is a drug widely used to treat heart failure and studies have demonstrated its potential as anticancer agent. In addition, digoxin presents the potential to interact with a series of other compounds used in medicine. The aim of the present study was to evaluate in vitro the cytotoxicity, genotoxicity and mutagenicity of digoxin and its potential to interact with the mutagen Mitomycin C (MMC). The cytotoxicity of digoxin was assessed by employing the MTT method and the comet assay was performed to assess the genotoxicity of this medicine in CHO-K1 and HeLa cell lines...
March 20, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28321776/colony-hanging-drop-and-methylcellulose-three-dimensional-hypoxic-growth-optimization-of-renal-cell-carcinoma-cell-lines
#6
Damian Matak, Klaudia K Brodaczewska, Monika Lipiec, Łukasz Szymanski, Cezary Szczylik, Anna M Czarnecka
Renal cell carcinoma (RCC) is the most lethal of the common urologic malignancies, comprising 3% of all human neoplasms, and the incidence of kidney cancer is rising annually. We need new approaches to target tumor cells that are resistant to current therapies and that give rise to recurrence and treatment failure. In this study, we focused on low oxygen tension and three-dimensional (3D) cell culture incorporation to develop a new RCC growth model. We used the hanging drop and colony formation methods, which are common in 3D culture, as well as a unique methylcellulose (MC) method...
March 20, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28260212/conservation-of-somatic-tissue-derived-from-collared-peccaries-pecari-tajacu-linnaeus-1758-using-direct-or-solid-surface-vitrification-techniques
#7
Alana Azevedo Borges, Gabriela Liberalino Lima, Luiza Bento de Queiroz Neta, Maria Valéria de Oliveira Santos, Moacir Franco de Oliveira, Alexandre Rodrigues Silva, Alexsandra Fernandes Pereira
Cryopreservation of somatic tissue can be applied in biodiversity conservation, especially for wild species as collared peccary. We aimed to evaluate the effect of vitrification techniques of ear tissue of collared peccary [direct vitrification in cryovials (DVC) or solid-surface vitrification (SSV)] on the layers of epidermis and dermis by conventional histology and cell ability during the in vitro culture. Thus, both the vitrification methods were able to maintain normal patterns of the epidermis as the cornea and granular layers, furthermore the intercellular space and dermal-epidermal junction of the spinous layer when compared to fresh control...
March 4, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28251404/the-enhancement-of-antibody-concentration-and-achievement-of-high-cell-density-cho-cell-cultivation-by-adding-nucleoside
#8
Yasuhiro Takagi, Takuya Kikuchi, Ryuta Wada, Takeshi Omasa
Recently, with the dramatic increase in demand for therapeutic antibodies, Chinese hamster ovary (CHO) cell culture systems have made significant progress in recombinant antibody production. Over the past two decades, recombinant antibody productivity has been improved by more than 100-fold. Medium optimization has been identified as an important key approach for increasing product concentrations. In this study, we evaluated the effects of deoxyuridine addition to fed-batch cultures of antibody-expressing CHO cell lines...
March 2, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28251403/induction-of-different-activated-phenotypes-of-mouse-peritoneal-macrophages-grown-in-different-tissue-culture-media
#9
Tomoya Kawakami, Atsushi Koike, Fumio Amano
The role of activated macrophages in the host defense against pathogens or tumor cells has been investigated extensively. Many researchers have been using various culture media in in vitro experiments using macrophages. We previously reported that J774.1/JA-4 macrophage-like cells showed great differences in their activated macrophage phenotypes, such as production of reactive oxygen, nitric oxide (NO) or cytokines depending on the culture medium used, either F-12 (Ham's F-12 nutrient mixture) or Dulbecco modified Eagle's medium (DMEM)...
March 1, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28214995/development-and-characterization-of-monoclonal-antibody-against-human-il-37b
#10
Yu-Chi Gao, Yan Jia, De-Qian Xiao, Xin Wang, You-Chao Dai, Shi-Yan Yu, Chen Chen, Ze-Gang Zhuang, Xiao-Xia Fu, Jun-Ai Zhang, Bi-Ying Zheng, Zhi-Hong Chen, Ji-Xin Zhong, Zhang-Quan Chen, Jun-Fa Xu
IL-37 has been described as a natural inhibitor of immune responses. Monoclonal antibody (mAb) against human IL-37b with high affinity and specificity can serve as a molecular probe to detect IL-37 and study IL-37 functions, mechanisms and related signal pathways in inflammatory diseases. However, there are very few such mAbs against human IL-37 commercially available so far. In the current study, monoclonal antibodies against human IL-37b were developed by fusing splenocytes from immunized mouse with SP2/0 myeloma cells and polyethylene glycol...
February 18, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28191587/cytotoxic-responses-of-carnosic-acid-and-doxorubicin-on-breast-cancer-cells-in-butterfly-shaped-microchips-in-comparison-to-2d-and-3d-culture
#11
Ece Yildiz-Ozturk, Sultan Gulce-Iz, Muge Anil, Ozlem Yesil-Celiktas
Two dimensional (2D) cell culture systems lack the ability to mimic in vivo conditions resulting in limitations for preclinical cell-based drug and toxicity screening assays and modelling tumor biology. Alternatively, 3D cell culture systems mimic the specificity of native tissue with better physiological integrity. In this regard, microfluidic chips have gained wide applicability for in vitro 3D cancer cell studies. The aim of this research was to develop a 3D biomimetic model comprising culture of breast cancer cells in butterfly-shaped microchip to determine the cytotoxicity of carnosic acid and doxorubicin on both estrogen dependent (MCF-7) and independent (MDA-MB231) breast cancer cells along with healthy mammary epithelial cells (MCF-10A) in 2D, 3D Matrigel™ and butterfly-shaped microchip environment...
February 13, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28188415/apoptosis-inducing-ability-of-silver-i-cyanide-phosphines-useful-for-anti-cancer-studies
#12
Zelinda Human-Engelbrecht, Reinout Meijboom, Marianne J Cronjé
Metal-based drugs have shown early promise as anticancer agents suggesting the potential application of silver(I) complexes as apoptosis-inducing agents. The ability of a silver(I) cyanide containing phosphine complex to induce cell death was evaluated in both a malignant (SNO esophageal cancer) and non-malignant (HDF-a skin and HEK293 kidney) cell lines. A dose-dependent decrease in cell viability was observed in the SNO cells. Light microscopy revealed morphological features indicative of apoptotic cell death...
February 10, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28181140/real-architecture-for-3d-tissue-raft%C3%A2-culture-system-improves-viability-and-maintains-insulin-and-glucagon-production-of-mouse-pancreatic-islet-cells
#13
Gabor J Szebeni, Zsuzsanna Tancos, Liliana Z Feher, Robert Alfoldi, Julianna Kobolak, Andras Dinnyes, Laszlo G Puskas
There is an unmet medical need for the improvement of pancreatic islet maintenance in culture. Due to restricted donor availability it is essential to ameliorate islet viability and graft engraftment. The aim of this study was to compare the standard tissue culture techniques with the advanced Real Architecture For 3D Tissue (RAFT™) culture system in terms of viability and hormone production. Here, we first report that islets embedded in RAFT™ collagen type I advanced tissue culture system maintain their tissue integrity better than in monolayer and suspension cultures...
February 8, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28181139/cytoprotective-and-anti-apoptotic-role-of-terminalia-arjuna-on-oxalate-injured-renal-epithelial-cells
#14
Amisha Mittal, Simran Tandon, Surender Kumar Singla, Chanderdeep Tandon
Urolithiasis is one of the painful multifactorial disorders caused by metabolic abnormalities influencing the composition of body fluids and urine. The bark of Terminalia arjuna (T. arjuna), very well known in Ayurveda for the treatment of cardiovascular diseases, possesses antioxidant and diuretic activity. The present study was undertaken to investigate the antiurolithiatic efficacy of aqueous extract of bark of T. arjuna on oxalate-induced injury to renal tubular epithelial cells. Madin-Darby canine kidney (MDCK) cells were exposed to 2 mM oxalate for 48 h to evaluate the protective effect of T...
February 8, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28132128/optimized-expression-of-the-antimicrobial-protein-gloverin-from-galleria-mellonella-using-stably-transformed-drosophila-melanogaster-s2-cells
#15
Jan Zitzmann, Tobias Weidner, Peter Czermak
Antimicrobial proteins and peptides (AMPs) are valuable as leads in the pharmaceutical industry for the development of novel anti-infective drugs. Here we describe the efficient heterologous expression and basic characterization of a Gloverin-family AMP derived from the greater wax moth Galleria mellonella. Highly productive single-cell clones prepared by limiting dilution achieved a 100% increase in productivity compared to the original polyclonal Drosophila melanogaster S2 cell line. Comprehensive screening for suitable expression conditions using statistical experimental designs revealed that optimal induction was achieved using 600 µM CuSO4 at the mid-exponential growth phase...
January 28, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28120140/lithium-chloride-promotes-neuronal-differentiation-of-rat-neural-stem-cells-and-enhances-neural-regeneration-in-parkinson-s-disease-model
#16
Li Qi, Yonggang Tang, Wei He, Honghua Pan, Wenxian Jiang, Lin Wang, Weilin Deng
Parkinson's disease (PD) is one of the most common neural degenerative disease, affecting millions of people globally. Great progress has been made in the PD treatment, and one of the most promising one is the stem cell-based therapy. Thus, studies on the differentiation of neural stem cells (NSCs) are important to the advancement in PD therapy. In this study, we used the rat NSCs to elucidate the role of Lithium in the proliferation and differentiation of NSCs by immunostaining against Ki67 and BrdU analysis as well as immunostaining against specific neuronal markers...
January 24, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28110386/an-optimization-protocol-for-swiss-3t3-feeder-cell-growth-arrest-by-mitomycin-c-dose-to-volume-derivation-strategy
#17
Rishi Man Chugh, Madhusudan Chaturvedi, Lakshmana Kumar Yerneni
Feeder cell functionality following growth-arrest with the cost-effective Mitomycin C vis-à-vis irradiation is controversial due to several methodological variables reported. Earlier, we demonstrated variability in growth arrested Swiss 3T3 feeder cell life-span following titration of feeder cell densities with Mitomycin C concentrations which led to the derivation of doses per cell. Alternatively, to counter the unexpected feeder regrowth at high exposure cell density, we proposed titration of a fixed density with arithmetically derived volumes of Mitomycin C solution that corresponded to permutations of specific concentrations and doses per cell...
January 21, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28101741/anti-hyperuricemic-effect-of-taxifolin-in-cultured-hepatocytes-and-model-mice
#18
Shin-Ichi Adachi, Ken-Ichi Nihei, Yoshiyuki Ishihara, Fumiaki Yoshizawa, Kazumi Yagasaki
Hyperuricemia is recognized as an important risk factor for gout. High dietary intake of purine-rich foods such as meats and sea foods increases uric acid (UA) levels in the blood. Taxifolin present in Siberian larch and strawberries has been reported to possess health promoting activities including anti-oxidant effect. In this study, we examined anti-hyperuricemic effect of taxifolin in both cultured hepatocytes and hyperuricemic model mice. In cultured AML12 hepatocytes, taxifolin significantly suppressed UA production dose- and time-dependently...
January 18, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28097454/in-vitro-cytogenetic-toxicity-of-bezafibrate-in-human-peripheral-blood-lymphocytes
#19
M Topaktas, N E Kafkas, S Sadighazadi, E S Istifli
Bezafibrate (BF) is a peroxisome proliferator-activated receptor (PPAR) agonist used as a lipid-lowering agent to treat both the familial or acquired combined forms of hyperlipidemia. BF is the only available fibrate drug that acts on all PPAR subtypes of α, β, and δ. Although there are studies that indicate a genotoxic potential associated with the use of fibrates, to our knowledge, the genotoxicity of BF in human peripheral blood lymphocytes has not been studied. In the present study, the genotoxic potential of BF was evaluated using chromosome aberration (CA) and micronucleus (MN) assays in peripheral blood lymphocytes of healthy human subjects...
January 17, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28084562/immunostimulatory-effect-of-dried-bonito-extract-on-mouse-macrophage-cell-lines-and-mouse-primary-peritoneal-macrophages
#20
Momoko Ishida, Kosuke Nishi, Kozue Shinohara, Nanami Kunihiro, Katsuhiro Osajima, Tomokazu Suemitsu, Takuya Sugahara
Dried bonito is a preserved food used in Japan, which contains abundant flavor ingredients and functional substances. We focused on the immunostimulatory effect of dried bonito extract (DBE) on mouse macrophage-like J774.1 cells, RAW264.7 cells, and mouse primary peritoneal macrophages. DBE significantly stimulated the production of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) by both J774.1 cells and peritoneal macrophages by enhancing the cytokine gene expression levels. In addition, DBE stimulated nitric oxide production by enhancing the expression of inducible nitric oxide synthase in RAW264...
January 13, 2017: Cytotechnology
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