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Cytotechnology

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https://www.readbyqxmd.com/read/28429166/lps-administration-increases-cd11b-c-fms-cd14-cell-population-that-possesses-osteoclast-differentiation-potential-in-mice
#1
Takuya Enomoto, Masamichi Takami, Matsuo Yamamoto, Ryutaro Kamijo
Osteoclasts are multinucleated giant cells that originate from a monocyte/macrophage lineage, and are involved in the inflammatory bone destruction accompanied by periodontitis. Recent studies have shown that osteoclast precursors reside not only in the bone marrow, but also in the peripheral blood and spleen, though the precise characteristics of each precursor have not been analyzed. We hypothesized that the number of osteoclast precursors in those tissues may increase under pathological conditions and contribute to osteoclast formation in vivo in a mouse model...
April 20, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28421411/seleno-short-chain-chitosan-induces-apoptosis-in-human-non-small-cell-lung-cancer-a549-cells-through-ros-mediated-mitochondrial-pathway
#2
Yana Zhao, Shaojing Zhang, Pengfei Wang, Shengnan Fu, Di Wu, Anjun Liu
Seleno-short-chain chitosan (SSCC) is a synthesized chitosan derivative. In this study, antitumor activity and underlying mechanism of SSCC on human non-small-cell lung cancer A549 cells were investigated in vitro. The MTT assay showed that SSCC could inhibit cell viability in a dose- and time-dependent manner, and 200 μg/ml SSCC exhibited significantly toxic effects on A549 cells. The cell cycle assay showed that SSCC triggered S phase cell cycle arrest in a dose- and time-dependent manner, which was related to a downregulation of S phase associated cyclin A...
April 18, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28409292/effects-of-the-donor-age-on-proliferation-senescence-and-osteogenic-capacity-of-human-urine-derived-stem-cells
#3
Peng Gao, Peilin Han, Dapeng Jiang, Shulong Yang, Qingbo Cui, Zhaozhu Li
To study the effects of the donor age on the application potential of human urine-derived stem cells (hUSCs) in bone tissue engineering, by comparing proliferation, senescence and osteogenic differentiation of hUSCs originated from volunteers with different ages. The urine samples were collected from 19 healthy volunteers (6 cases from children group aged from 5 to 14, 5 cases from middle-aged group aged from 30 to 40, and 8 cases from the elder group aged from 65 to 75), and hUSCs were isolated and cultured...
April 13, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28397099/maturation-of-human-pluripotent-stem-cell-derived-cardiomyocytes-is-improved-in-cardiovascular-construct
#4
Hanna Vuorenpää, Kirsi Penttinen, Tuula Heinonen, Mari Pekkanen-Mattila, Jertta-Riina Sarkanen, Timo Ylikomi, Katriina Aalto-Setälä
In order to translate preclinical data into the clinical studies, relevant in vitro models with structure and key functional properties similar to native human tissue should be used. In vitro cardiac models with vascular structures mimic the highly vascularized myocardium and provide interactions between endothelial cells, stromal cells and cardiomyocytes. Currently, human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have been shown to present immature morphology and fetal-like electrophysiological properties that may limit their use as physiological test platform...
April 10, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28397098/persian-shallot-allium-hirtifolium-boiss-induced-apoptosis-in-human-hepatocellular-carcinoma-cells
#5
Farzaneh Sadat Hosseini, Soudeh Khanamani Falahati-Pour, Mohammad Reza Hajizadeh, Alireza Khoshdel, Mohammad Reza Mirzaei, Hadis Ahmadirad, Reza Behroozi, Nesa Jafari, Mehdi Mahmoodi
This study investigated the potential of Persian shallot extract as an anticancer agent in HepG2 tumor cell line, an in vitro human hepatoma cancer model system. The inhibitory effect of Persian shallot on the growth of HepG2 cells was measured by MTT assay. To explore the underlying mechanism of cell growth inhibition of Persian shallot, the activity of Persian shallot in inducing apoptosis was investigated through the detection of annexin V signal by flow cytometry and expression of some apoptosis related genes such p21, p53, puma, caspase-8 family-Bcl-2 proteins like bid, bim, bcl-2 and bax were measured by real-time PCR in HepG2 cells...
April 10, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28393288/differential-effects-of-p38-map-kinase-inhibitors-sb203580-and-sb202190-on-growth-and-migration-of-human-mda-mb-231-cancer-cell-line
#6
Şükrü Aydın Düzgün, Azmi Yerlikaya, Sezgin Zeren, Zülfü Bayhan, Emrah Okur, İhsan Boyacı
p38 mitogen-activated protein kinase (MAPK) belongs to the MAPK superfamily, phosphorylating serine and/or threonine residues of the target proteins. The activation of p38 MAPK leads to cell growth, differentiation, inflammation, survival or apoptosis. In this study, we tested the effect of two highly specific and potent inhibitors of p38 MAPK (namely, SB203580 and SB202190) on human breast cancer cell line MDA-MB-231 to elucidate the controversial role of p38 MAPK on cell proliferation and/or cell migration/metastasis further...
April 9, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28386771/potential-neoplastic-evolution-of-vero-cells-in-vivo-and-in-vitro-characterization
#7
N A Andreani, S Renzi, G Piovani, P Ajmone Marsan, L Bomba, R Villa, M Ferrari, S Dotti
Vero cell lines are extensively employed in viral vaccine manufacturing. Similarly to all established cells, mutations can occur during Vero cells in vitro amplification which can result in adverse features compromising their biological safety. To evaluate the potential neoplastic evolution of these cells, in vitro transformation test, gene expression analysis and karyotyping were compared among low- (127 and 139 passages) and high-passage (passage 194) cell lines, as well as transformed colonies (TCs). In vivo tumorigenicity was also tested to confirm preliminary in vitro data obtained for low passage lines and TCs...
April 6, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28374108/geraniin-suppresses-tumor-cell-growth-and-triggers-apoptosis-in-human-glioma-via-inhibition-of-stat3-signaling
#8
Zhong Ren, Wenshuang Zou, Junfeng Cui, Luping Liu, Yang Qing, Yongmei Li
Natural phytochemicals are attracting increasing interest as anticancer agents. The aim of this study is to evaluate the therapeutic potential of geraniin, a major ellagitannin extracted from Geranium sibiricum L., in human glioma. Human U87 and LN229 glioma cells were treated with different concentrations of geraniin, and cell viability, apoptosis, and gene expression were assessed. The involvement of STAT3 signaling in the action of geraniin was examined. We found that geraniin treatment for 48 h significantly (P < 0...
April 3, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28374107/carnosine-activates-the-creb-pathway-in-caco-2-cells
#9
Kaoru Fujii, Kayoko Abe, Keishi Kadooka, Takashi Matsumoto, Yoshinori Katakura
Imidazole dipeptide, carnosine, is a versatile compound composed of β-Ala and L-His. A recent study showed that carnosine might benefit the treatment of Alzheimer's disease and the maintenance of cognitive function. Based on the observation that carnosine is immediately degraded by serum carnosinase, we hypothesized that carnosine improves brain function by promoting brain-gut interaction. This study sought to present possible mechanisms regulating carnosine-induced activation of brain-gut interaction. We had previously found that carnosine augmented the expression of BDNF in human colorectal cancer cells, thus we became interested in cAMP-responsive element binding protein (CREB), which is a dominant regulator of BDNF transcription...
April 3, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28365799/establishment-of-insect-cell-lines-expressing-green-fluorescent-protein-on-cell-surface-based-on-acmnpv-gp64-membrane-fusion-characteristic
#10
Ben-Xiang Qi, Ying-Jian Chen, Rui Su, Yi-Fei Li, Gui-Ling Zheng, Chang-You Li
Displaying a protein on the surface of cells has been provided a very successful strategy to function research of exogenous proteins. Based on the membrane fusion characteristic of Autographa californica multiple nucleopolyhedrovirus envelope protein GP64, we amplified and cloned N-terminal signal peptide and C-terminal transmembrane domain as well as cytoplasmic tail domain of gp64 gene into vector pIZ/V5-His with multi-cloning sites to construct the cell surface expression vector pIZ/V5-gp64. To verify that the vector can be used to express proteins on the membrane of insect cells, a recombinant plasmid pIZ/V5-gp64-GFP was constructed by introducing the PCR amplified green fluorescent protein (GFP) gene and transfected into insect cell lines Sf9 and H5...
April 1, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28342004/modulatory-effects-of-terminalia-arjuna-against-domoic-acid-induced-toxicity-in-caco-2-cell-line
#11
E M Ramya, G Phani Kumar, T Anand, K R Anilakumar
Domoic acid is a potent marine algal toxin produced by diatomic genus of Pseudo-nitzschia causing amnesic shell fish poisoning. Domoic acid toxicosis mainly involves excitotoxic effects coupled with oxidative stress. The present study was aimed to evaluate the protective effects of hydro-alcoholic extract of Terminalia arjuna (TA) against domoic acid induced toxic effects in Caco-2 cell line. It was observed that the toxicity induced by domoic acid in Caco-2 cells was mediated by oxidative insult leading to morphological changes, DNA damage and apoptosis...
March 24, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28337561/optimization-of-ultraviolet-ozone-treatment-process-for-improvement-of-polycaprolactone-pcl-microcarrier-performance
#12
Nurhusna Samsudin, Yumi Zuhanis Has-Yun Hashim, Mohd Azmir Arifin, Maizirwan Mel, Hamzah Mohd Salleh, Iis Sopyan, Dzun Noraini Jimat
Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the surface functionality of certain polymeric microcarriers for effective cell attachment and growth remains a challenge. Polycaprolactone (PCL), a biodegradable polymer has received considerable attention due to its good mechanical properties and degradation rate. The drawback is the non-polar hydrocarbon moiety which makes it not readily suitable for cell attachment. This report concerns the modification of PCL microcarrier surface (introduction of functional oxygen groups) using ultraviolet irradiation and ozone (UV/O3) system and investigation of the effects of ozone concentration, the amount of PCL and exposure time; where the optimum conditions were found to be at 60,110...
March 23, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28321780/isolation-culture-and-identification-of-pulmonary-arterial-smooth-muscle-cells-from-rat-distal-pulmonary-arteries
#13
Gongyong Peng, Juan Xu, Rongmin Liu, Zhenli Fu, Shaoxing Li, Wei Hong, Jinglong Chen, Bing Li, Pixin Ran
The culture of pulmonary arterial smooth muscle cells (PASMCs) is one of the most powerful tools for exploring the mechanisms of pulmonary hypertension (PH). Both pulmonary vasoconstriction and remodeling occur predominantly in distal pulmonary arteries (PA). In this study, we provide our detailed and standardized protocol for easy isolation and culture of PASMCs from rat distal PA to supply every investigator with a simple, economical and useful method in studying PH. The protocol can be divided into four stages: isolation of distal PA, isolation of cells, growth in culture and passage of cells...
March 21, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28321779/transfection-of-bovine-fetal-fibroblast-with-polyethylenimine-pei-nanoparticles-effect-of-particle-size-and-presence-of-fetal-bovine-serum-on-transgene-delivery-and-cytotoxicity
#14
D O Forcato, A E Fili, F E Alustiza, J M Lázaro Martínez, S Bongiovanni Abel, M F Olmos Nicotra, A P Alessio, N Rodríguez, C Barbero, P Bosch
The development of efficient transfection protocols for livestock cells is crucial for implementation of cell-based transgenic methods to produce genetically modified animals. We synthetized fully deacylated linear 22, 87 and 217 kDa polyethylenimine (PEI) nanoparticles and compared their transfection efficiency and cytotoxicity to commercial branched 25 kDa PEI and linear 58 kDa poly(allylamine) hydrochloride. We studied the effect of PEI size and presence of serum on transfection efficiency on primary cultures of bovine fetal fibroblasts and established cells lines (HEK 293 and Hep G2)...
March 20, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28321778/effects-of-mir-21-downregulation-and-silibinin-treatment-in-breast-cancer-cell-lines
#15
Zohreh Jahanafrooz, Nasrin Motamed, Behnaz Bakhshandeh
Silibinin is a natural polyphenol with high antioxidant and anticancer properties, which causes cell cycle arrest and apoptosis in most cancer cell types including breast cancer, but the in-line mechanisms, are still unknown. Silibinin significantly downregulated oncomiR miR-21 expression in breast cancer cells. Here the effect of anti-miR-21 on cell viability, apoptotic induction, cell cycle distribution, and the expression levels of downstream targets of miR-21 were investigated in MCF-7 and T47D cells. MiR-21 mimic transfection was also applied in silibinin treated samples to evaluate functional role of miR-21downregulation on silibinin effects...
March 20, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28321777/digoxin-reduces-the-mutagenic-effects-of-mitomycin-c-in-human-and-rodent-cell-lines
#16
Júlia Teixeira de Oliveira, Maria C da Silva Barbosa, Luiz F de Camargos, Isabella Viana Gomes da Silva, Fernando de Pilla Varotti, Luciana M da Silva, Leonardo Marmo Moreira, Juliana Pereira Lyon, Vanessa J da Silva Vieira Dos Santos, Fabio Vieira Dos Santos
Digoxin is a drug widely used to treat heart failure and studies have demonstrated its potential as anticancer agent. In addition, digoxin presents the potential to interact with a series of other compounds used in medicine. The aim of the present study was to evaluate in vitro the cytotoxicity, genotoxicity and mutagenicity of digoxin and its potential to interact with the mutagen Mitomycin C (MMC). The cytotoxicity of digoxin was assessed by employing the MTT method and the comet assay was performed to assess the genotoxicity of this medicine in CHO-K1 and HeLa cell lines...
March 20, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28321776/colony-hanging-drop-and-methylcellulose-three-dimensional-hypoxic-growth-optimization-of-renal-cell-carcinoma-cell-lines
#17
Damian Matak, Klaudia K Brodaczewska, Monika Lipiec, Łukasz Szymanski, Cezary Szczylik, Anna M Czarnecka
Renal cell carcinoma (RCC) is the most lethal of the common urologic malignancies, comprising 3% of all human neoplasms, and the incidence of kidney cancer is rising annually. We need new approaches to target tumor cells that are resistant to current therapies and that give rise to recurrence and treatment failure. In this study, we focused on low oxygen tension and three-dimensional (3D) cell culture incorporation to develop a new RCC growth model. We used the hanging drop and colony formation methods, which are common in 3D culture, as well as a unique methylcellulose (MC) method...
March 20, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28260212/conservation-of-somatic-tissue-derived-from-collared-peccaries-pecari-tajacu-linnaeus-1758-using-direct-or-solid-surface-vitrification-techniques
#18
Alana Azevedo Borges, Gabriela Liberalino Lima, Luiza Bento de Queiroz Neta, Maria Valéria de Oliveira Santos, Moacir Franco de Oliveira, Alexandre Rodrigues Silva, Alexsandra Fernandes Pereira
Cryopreservation of somatic tissue can be applied in biodiversity conservation, especially for wild species as collared peccary. We aimed to evaluate the effect of vitrification techniques of ear tissue of collared peccary [direct vitrification in cryovials (DVC) or solid-surface vitrification (SSV)] on the layers of epidermis and dermis by conventional histology and cell ability during the in vitro culture. Thus, both the vitrification methods were able to maintain normal patterns of the epidermis as the cornea and granular layers, furthermore the intercellular space and dermal-epidermal junction of the spinous layer when compared to fresh control...
March 4, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28251404/the-enhancement-of-antibody-concentration-and-achievement-of-high-cell-density-cho-cell-cultivation-by-adding-nucleoside
#19
Yasuhiro Takagi, Takuya Kikuchi, Ryuta Wada, Takeshi Omasa
Recently, with the dramatic increase in demand for therapeutic antibodies, Chinese hamster ovary (CHO) cell culture systems have made significant progress in recombinant antibody production. Over the past two decades, recombinant antibody productivity has been improved by more than 100-fold. Medium optimization has been identified as an important key approach for increasing product concentrations. In this study, we evaluated the effects of deoxyuridine addition to fed-batch cultures of antibody-expressing CHO cell lines...
March 2, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28251403/induction-of-different-activated-phenotypes-of-mouse-peritoneal-macrophages-grown-in-different-tissue-culture-media
#20
Tomoya Kawakami, Atsushi Koike, Fumio Amano
The role of activated macrophages in the host defense against pathogens or tumor cells has been investigated extensively. Many researchers have been using various culture media in in vitro experiments using macrophages. We previously reported that J774.1/JA-4 macrophage-like cells showed great differences in their activated macrophage phenotypes, such as production of reactive oxygen, nitric oxide (NO) or cytokines depending on the culture medium used, either F-12 (Ham's F-12 nutrient mixture) or Dulbecco modified Eagle's medium (DMEM)...
March 1, 2017: Cytotechnology
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