journal
MENU ▼
Read by QxMD icon Read
search

Cytotechnology

journal
https://www.readbyqxmd.com/read/29352392/impact-of-dexamethasone-concentration-on-cartilage-tissue-formation-from-human-synovial-derived-stem-cells-in-vitro
#1
Ryota Chijimatsu, Masato Kobayashi, Kosuke Ebina, Toru Iwahashi, Yosuke Okuno, Makoto Hirao, Atsunori Fukuhara, Norimasa Nakamura, Hideki Yoshikawa
Human synovial mesenchymal stem cells (hSMSCs) are a promising cell source for cartilage regeneration because of their superior chondrogenic potential in vitro. This study aimed to further optimize the conditions for inducing chondrogenesis of hSMSCs, focusing on the dose of dexamethasone in combination with transforming growth factor-β3 (TGFβ3) and/or bone morphogenetic protein-2 (BMP2). When hSMSCs-derived aggregates were cultured with TGFβ3, dexamethasone up to 10 nM promoted chondrogenesis, but attenuated it with heterogeneous tissue formation when used at concentrations over than 100 nM...
January 19, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29352391/functional-recovery-by-application-of-human-dedifferentiated-fat-cells-on-cerebral-infarction-mice-model
#2
Tomoki Kakudo, Naotaka Kishimoto, Tomohiro Matsuyama, Yoshihiro Momota
Elderly people whose daily activities have declined due to a cerebrovascular disorder may suffer from dysphagia and may find oral hygiene difficult. Therefore, it is important to establish an effective therapy for the underlying cerebrovascular disorder. Dedifferentiated fat cells (DFAT) were obtained from mature adipocytes isolated from human buccal adipose pads in a ceiling culture. DFAT expressed the neural markers Nestin and SOX2. Flow cytometric analysis revealed that the cells had properties similar to mesenchymal stem cells...
January 19, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29350306/progress-on-the-study-of-the-mechanism-of-busulfan-cytotoxicity
#3
REVIEW
Xiaoli Chen, Mingyuan Liang, Dong Wang
The preparation of spermatogonial stem cell (SSC) transplant recipients laid the technical foundation for SSC transplant technology and the understanding of spermatogenesis mechanisms. Busulfan is commonly used to prepare recipients for mouse SSC transplantation; however, its safety and efficiency have been questioned. This review summarizes the relationship between SSCs and Sertoli cells (SCs), and the mechanism of busulfan toxicity against sperm cells. We concluded that the proliferation, differentiation, and apoptosis of SSCs are regulated by SCs...
January 19, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29349583/enhanced-production-of-phenolic-acids-in-cell-suspension-culture-of-salvia-leriifolia-benth-using-growth-regulators-and-sucrose
#4
Masoomeh Modarres, Sedigheh Esmaeilzadeh Bahabadi, Mohammad Ehsan Taghavizadeh Yazdi
Salvia leriifolia Benth. (Lamiaceae) is an endangered medicinal plant with hypoglycemic, anti-inflammatory and analgesic properties. Many of the beneficial effects of Salvia spp. are attributed to the phenolic compounds. In the present study, an efficient procedure has been developed for establishment of cell suspension culture of S. leriifolia as a strategy to obtain an in vitro phenolic acids producing cell line for the first time. The effect of growth regulators and various concentrations of sucrose have been analyzed, to optimize biomass growth and phenolic acids production...
January 18, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29344745/wet-milling-of-large-quantities-of-human-excision-adipose-tissue-for-the-isolation-of-stromal-vascular-fraction-cells
#5
Nadia Menzi, Rik Osinga, Atanas Todorov, Dirk Johannes Schaefer, Ivan Martin, Arnaud Scherberich
The isolation of stromal vascular fraction (SVF) cells from excised human adipose tissue, for clinical or research purposes, implies the tedious and time consuming process of manual mincing prior to enzymatic digestion. Since no efficient alternative technique to this current standard procedure has been proposed so far, the aim of this study was to test a milling procedure, using two simple, inexpensive and commercially available manual meat grinders, to process large amounts of adipose tissue. The procedure was assessed on adipose tissue resections from seven human donors and compared to manual mincing with scalpels...
January 17, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29340836/primary-culture-of-lung-fibroblasts-from-hyperoxia-exposed-rats-and-a-proliferative-characteristics-study
#6
Shi-Meng Zhao, Hong-Min Wu, Mei-Ling Cao, Dan Han
Lung fibrosis is an ultimate consequence of bronchopulmonary dysplasia (BPD) which shows the excessive proliferation of lung fibroblasts (LFs). To find a better model for studying the role of LFs in hyperoxia-induced lung fibrosis at the cellular level, we isolated LFs from the lung tissue of hyperoxia- and normoxia-exposed rat lungs on postnatal days 7, 14 and 21 for primary culture to study their proliferative behavior. In the present study, the LF predominance was > 95% in our culture method. The LFs isolated from rats exposed to hyperoxia in vivo showed significantly greater proliferation than that from normoxia-exposed rats...
January 16, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29335808/perfluorooctanoic-acid-impaired-glucose-homeostasis-through-affecting-adipose-akt-pathway
#7
Gang Du, Jinhong Sun, Yang Zhang
Perfluorooctanoic acid (PFOA) is commonly applied in manufactured products, and its potential health risk is concerned greatly. Increasing evidences have indicated PFOA-induced liver dysfunction. However, detailed molecular mechanism has not been completely identified. In this study, we aimed to investigate the mechanical association between PFOA exposure and AKT pathway in white adipose tissue. As results, PFOA-treated mice showed increased blood glucose and insulin levels, and induced insulin resistance. In addition, serum levels of leptin and adiponectin in PFOA-treated mice were elevated...
January 16, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29335807/assessment-of-in-vitro-cytotoxic-and-genotoxic-activities-of-some-trimethoprim-conjugates
#8
Devrim Güzel Bayülken, R Beklem Bostancıoğlu, A Tansu Koparal, Berrin Ayaz Tüylü, Aydan Dağ, Kadriye Benkli
Trimethoprim, a commonly used antibacterial agent, is widely applied in the treatment of variety of infections in human. A few studies have demonstrated an extensive exposure of man to antibiotics, but there is still a lack of data for cytotoxic effects including nephrotoxicity, gastrointestinal toxicity, hematotoxicity, neurotoxicity and ototoxicity. The main purpose behind this study was to determine cytotoxic and genotoxic activities of trimethoprim (1), trimethoprim with maleic acid (2) and trimethoprim in conjugation with oxalic acid dihydrate (3)...
January 15, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29322349/development-of-a-chemically-defined-platform-fed-batch-culture-media-for-monoclonal-antibody-producing-cho-cell-lines-with-optimized-choline-content
#9
Shinobu Kuwae, Ichiko Miyakawa, Tomohiro Doi
A chemically defined platform basal medium and feed media were developed using a single Chinese hamster ovary (CHO) cell line that produces a monoclonal antibody (mAb). Cell line A, which showed a peak viable cell density of 5.9 × 106 cells/mL and a final mAb titer of 0.5 g/L in batch culture, was selected for the platform media development. Stoichiometrically balanced feed media were developed using glucose as an indicator of cell metabolism to determine the feed rates of all other nutrients. A fed-batch culture of cell line A using the platform fed-batch medium yielded a 6...
January 11, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29322348/optimisation-of-a-potency-assay-for-the-assessment-of-immunomodulative-potential-of-clinical-grade-multipotent-mesenchymal-stromal-cells
#10
Irene Oliver-Vila, Carmen Ramírez-Moncayo, Marta Grau-Vorster, Sílvia Marín-Gallén, Marta Caminal, Joaquim Vives
Clinical use of multipotent Mesenchymal Stromal Cell (MSC)-based medicinal products requires their production in compliance with Good Manufacturing Practices, thus ensuring that the final drug product meets specifications consistently from batch to batch in terms of cell viability, identity, purity and potency. Potency relates to the efficacy of the medicine in its target clinical indication, so adequate release tests need to be defined and validated as quality controls. Herein we report the design and optimisation of parameters affecting the performance of an in vitro cell-based assay for assessing immunomodulatory potential of clinical grade MSC for human use, based on their capacity to inhibit proliferation of T lymphocytes under strong polyclonal stimuli...
January 10, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29322347/all-aspect-of-toxic-effect-of-brilliant-blue-and-sunset-yellow-in-allium-cepa-roots
#11
Kemal Koç, Dilek Pandir
Substances added to food are considerable for survival and are the oldest technologies used in preservation, sweetening and coloring. This work was conducted to evaluate the toxicity of the food additives sunset yellow (SY) and brilliant blue (BB) on Allium cepa root meristematic cells. Control and treatment groups were created from germinated roots. Group 1 (control group) did not receive chemicals. Group 2 (SY or BB-treatment group), received increasing doses of SY (25, 50, 100 and 500 ppm) and BB (100, 200, 400 and 500 ppm) with time periods of 24, 48 and 72 h...
January 10, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29305674/comparable-osteogenic-capacity-of-mesenchymal-stem-or-stromal-cells-derived-from-human-amnion-membrane-and-bone-marrow
#12
Mehran Ghasemzadeh, Ehteramolsadat Hosseini, Mohammadhossein Ahmadi, Maedeh Kamalizad, Naser Amirizadeh
So far, substantial attentions have been attracted to the application of mesenchymal stem or stromal cells (MSCs) in different therapeutic approaches. Although human bone marrow is commonly considered as a major source for MSCs, having an invasive collection method, ethical consideration and donor availability create a challenge for scientists, leading them to explore better alternative sources for MSCs. The study presented here aimed to characterize and compare osteogenic capacity of MSCs obtained from the amnion membrane (AM) with those originated from BM...
January 5, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29264678/long-term-culture-and-differentiation-of-endothelial-progenitor-like-cells-from-rat-adipose-derived-stem-cells
#13
Monire Amerion, Mojtaba Rezazadeh Valojerdi, Saeid Abroun, Mehdi Totonchi
The procedure of obtaining qualified endothelial progenitor cells (EPCs) is still unclear and there has been some controversy on their biological properties and time of emergence. In this study, we used long-term culture of Adipose Derived Stem Cells (ADSCs) in an endothelial induction medium to obtain endothelial progenitor-like cells, and investigated the features of a few surface markers and the physiologic functions of the cells produced. In order to achieve our aim, rat ADSCs were isolated and cultured in an endothelial basal medium (EBM2), supplemented with an endothelial growth medium (EGM2)...
December 20, 2017: Cytotechnology
https://www.readbyqxmd.com/read/29234945/clinical-significance-and-functional-validation-of-inorganic-pyrophosphatase-in-diffuse-large-b-cell-lymphoma-in-humans
#14
Limei Li, Aruna, Dehong Luo, Arong Jin
Diffuse large B-cell lymphoma (DLBCL), the most common type of non-Hodgkin's lymphoma, displays marked heterogeneity. Although it is usually curable, 30-40% of patients die within 1-2 years due to refractory treatment or cancer relapse. In different types of cancer in humans, inorganic pyrophosphatase (PPA1) is deregulated, thereby contributing to tumorigenesis by supplying the tumor with an enormous energy source. However, the role of PPA1 in DLBCL is still unclear. Here, we analized PPA1 in 65 patients with DLBCL and 20 patients with reactive hyperplasia of the lymph nodes (control)...
December 12, 2017: Cytotechnology
https://www.readbyqxmd.com/read/29234944/investigating-the-mincing-method-for-isolation-of-adipose-derived-stem-cells-from-pregnant-women-fat
#15
Yuan-Sheng Li, Pao-Jen Chen, Li-Wei Wu, Pei-Wen Chou, Li-Yi Sun, Tzyy-Wen Chiou
The success of stem cell application in regenerative medicine, usually require a stable source of stem or progenitor cells. Fat tissue represents a good source of stem cells because it is rich in stem cells and there are fewer ethical issues related to the use of such stem cells, unlike embryonic stem cells. Therefore, there has been increased interest in adipose-derived stem cells (ADSCs) for tissue engineering applications. Here, we aim to provide an easy processing method for isolating adult stem cells from human adipose tissue harvested from the subcutaneous fat of the abdominal wall during gynecologic surgery...
December 12, 2017: Cytotechnology
https://www.readbyqxmd.com/read/29230632/ghrelin-accelerates-the-cartilagic-differentiation-of-rabbit-mesenchymal-stem-cells-through-the-erk1-2-pathway
#16
Nan Ye, Lin Wang, Zhe Dou, Jian Huang
Mesenchymal stem cells (MSCs) can differentiate into chondroblasts, adipocytes, or cartilage under appropriate stimulation. Identifying a mechanism triggering the differentiation of MSCs into cartilage may help develop novel therapeutic approaches for treating heterotopic ossification, the pathological formation of lamellar bone in soft tissue outside the skeleton that can lead to debilitating immobility. Ghrelin, an endogenous ligand for the growth hormone secretagogue receptor, stimulates growth hormone secretion, and has both orexigenic and adipogenic effects...
December 12, 2017: Cytotechnology
https://www.readbyqxmd.com/read/29230631/the-investigation-of-ceranib-2-on-apoptosis-and-drug-interaction-with-carboplatin-in-human-non-small-cell-lung-cancer-cells-in-vitro
#17
Merve Yildiz-Ozer, Pinar Oztopcu-Vatan, Gokhan Kus
Ceramide is found to be involved in inhibition of cell division and induction of apoptosis in certain tumour cells. Ceranib-2 is an agent that increases ceramide levels by inhibiting ceramidase in cancer cells. Therefore, we aimed to investigate the effects of ceranib-2 on cell survival, apoptosis and interaction with carboplatin in human non-small cell lung cancer cells. The cytotoxic effect of ceranib-2 (1-100 µM) was determined by MTT assay in human lung adenocarcinoma (A549) and large cell lung carcinoma (H460) cells...
December 11, 2017: Cytotechnology
https://www.readbyqxmd.com/read/29230630/phytochemical-study-and-evaluation-of-cytotoxicity-mutagenicity-cell-cycle-kinetics-and-gene-expression-of-bauhinia-holophylla-bong-steud-in-hepg2-cells-in-vitro
#18
Diego Luis Ribeiro, Heloísa Lizotti Cilião, Ana Flávia Leal Specian, Juliana Mara Serpeloni, Marcelo Tempesta De Oliveira, Eliana Aparecida Varanda, Wagner Vilegas, Luiz Leonardo Saldanha, Wilner Martínez-López, Anne Lígia Dokkedal, Ilce Mara Syllos Cólus
Bauhinia holophylla (Bong.) Steud. (Fabaceae) is a plant used in Brazilian folk medicine to treat diabetes and inflammation. This study evaluated the phytochemical properties, cytotoxic, apoptotic, mutagenic/antimutagenic effects and alterations in gene expression (RNAm) in HepG2 cells treated with the B. holophylla extract. The phytochemical profile highlight the presence of flavonoids isorhamentin and quercetin derivates. The MTT assay was used to evaluate the cytotoxicity of different concentrations for different treatment times...
December 11, 2017: Cytotechnology
https://www.readbyqxmd.com/read/29204944/comparison-of-culture-media-indicates-a-role-for-autologous-serum-in-enhancing-phenotypic-preservation-of-rabbit-limbal-stem-cells-in-explant-culture
#19
Mehmet Gürdal, Özlem Barut Selver, Kemal Baysal, İsmet Durak
In this study, we aimed to compare the effects of six different cell culture media and autologous serum (AS) on the phenotypic characteristics of rabbit limbal epithelial stem cells (LESC) cultivated on porous polyethylene terephthalate (PET) membranes. Limbal explants from rabbit corneas were grown on PET membrane inserts in five different media: DMEM-F12 with fetal bovine serum (FBS) (DMEM-F12-FBS), with pluripotin (DMEM-F12-pluripotin) and with autologous serum (DMEM-F12-AS), Epilife, Keratinocyte Serum Free Medium (KSFM) and Defined-Keratinocyte Serum Free Medium...
December 4, 2017: Cytotechnology
https://www.readbyqxmd.com/read/29189979/comparison-of-biophysical-properties-characterized-for-microtissues-cultured-using-microencapsulation-and-liquid-crystal-based-3d-cell-culture-techniques
#20
Chin Fhong Soon, Kian Sek Tee, Soon Chuan Wong, Nafarizal Nayan, Sargunan Sundra, Mohd Khairul Ahmad, Farshid Sefat, Naznin Sultana, Mansour Youseffi
Growing three dimensional (3D) cells is an emerging research in tissue engineering. Biophysical properties of the 3D cells regulate the cells growth, drug diffusion dynamics and gene expressions. Scaffold based or scaffoldless techniques for 3D cell cultures are rarely being compared in terms of the physical features of the microtissues produced. The biophysical properties of the microtissues cultured using scaffold based microencapsulation by flicking and scaffoldless liquid crystal (LC) based techniques were characterized...
November 30, 2017: Cytotechnology
journal
journal
29867
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"