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Cytotechnology

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https://www.readbyqxmd.com/read/28625011/liver-epithelioid-progenitor-cells-derived-from-fetal-luxi-bovine-alleviate-liver-fibrosis
#1
Kunfu Wang, Hao Liu, Jinjuan Yang, Caiyun Ma, Zebiao Zhang, Dong Zheng, Weijun Guan
Liver epithelioid progenitor cells (LEPCs) have important roles in liver therapy because of their hepatic differentiation potency in vitro and in vivo. Despite many researches on humans, mice, and rats, equivalent progenitor cells derived from bovine are relatively rare. The purpose of our current study is to characterize bovine LEPCs, and research on the cure potency of this heteroplastic progenitor cells on mice liver fibrosis. We have used collagenase IV digesting and differential adhesion method to isolate slabstone shape, EpCAM, LGR5, NCAM1 and SOX9 positive progenitor cells from fetal Luxi bovine liver...
June 17, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28608258/extracts-of-chrysanthemum-zawadskii-attenuate-oxidative-damage-to-vascular-endothelial-cells-caused-by-a-highly-reducing-sugar
#2
Hyun-Sook Kim
Endothelial cells are considered candidates for involvement in the pathogenesis of diabetic vascular complications, and prevention of endothelial cell damage may be important in pharmacological attempts to prevent such complications. In the present study, I explored whether extracts of Chrysanthemum zawadskii (CZE) could prevent oxidative damage and dysfunction of a vascular endothelial cell line caused by the highly reducing sugar, 2-deoxy-D-ribose (dRib), and dysfunction of a vascular endothelial cell line...
June 12, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28608257/mutagenic-and-genotoxic-effects-of-anilofos-with-micronucleus-chromosome-aberrations-sister-chromatid-exchanges-and-ames-test
#3
Dilek Akyil, Muhsin Konuk, Yasin Eren, Recep Liman, Esra Sağlam
We aimed to evaluate the mutagenic effect of Anilofos, organophosphate pesticide, by using Ames/Salmonella/microsome test. Its cytotoxic and genotoxic effects were also determined by chromosome aberration (CA), sister chromatid exchange (SCE) and micronucleus (MN) test in human peripheral blood lymphocytes. In the Ames test, five different concentrations of Anilofos were examined on TA97, TA98, TA100 and TA102 strains in the absence and presence of S9 fraction. According to the results all concentrations of this pesticide have not shown any mutagenic activity on TA97, TA100 and TA102 strains in the absence and presence of S9 fraction...
June 12, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28601931/cardiotrophin-1-stimulates-the-neural-differentiation-of-human-umbilical-cord-blood-derived-mesenchymal-stem-cells-and-survival-of-differentiated-cells-through-pi3k-akt-dependent-signaling-pathways
#4
Longying Peng, Xiaomei Shu, Changhui Lang, Xiaohua Yu
Cardiotrophin-1 (CT1) plays an important role in the differentiation, development, and survival of neural stem cells. In this study, we analyzed its effects on the stimulation of human umbilical cord blood-derived mesenchymal stem cells in terms of their potential to differentiate into neuron-like cells, their survival characteristics, and the molecular mechanisms involved. The treatment of cells with neural induction medium (NIM) and CT1 generated more cells that were neuron-like and produced stronger expression of neural-lineage markers than cells treated with NIM and without CT1...
June 10, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28584932/modifications-of-a-signal-sequence-for-antibody-secretion-from-insect-cells
#5
Yuki Ohmuro-Matsuyama, Hideki Yamaji
Monoclonal antibodies and antibody fragments have recently been developed for use in diverse diagnostic and therapeutic applications. Insect cells can efficiently secrete recombinant proteins such as antibody molecules through post-translational processing and modifications that are similar to those performed in mammalian cells. In eukaryotic cells, the signal sequence in a nascent polypeptide is recognized by the signal recognition particle, and the polypeptide is then folded and modified in the endoplasmic reticulum...
June 5, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28573544/lipocalin-2-enhances-mesenchymal-stem-cell-based-cell-therapy-in-acute-kidney-injury-rat-model
#6
Mehryar Habibi Roudkenar, Raheleh Halabian, Hossein Abdul Tehrani, Fatemeh Amiri, Ali Jahanian-Najafabadi, Amaneh Mohammadi Roushandeh, Zahra Abbasi-Malati, Yoshikazu Kuwahara
Acute kidney injury (AKI) is one of the most common health-threatening diseases in the world. There is still no effective medical treatment for AKI. Recently, Mesenchymal stem cell (MSC)-based therapy has been proposed for treatment of AKI. However, the microenvironment of damaged kidney tissue is not favorable for survival of MSCs which would be used for therapeutic intervention. In this study, we genetically manipulated MSCs to up-regulate lipocalin-2 (Lcn2) and investigated whether the engineered MSCs (MSC-Lcn2) could improve cisplatin-induced AKI in a rat model...
June 2, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28551826/development-of-an-8-color-antibody-panel-for-functional-phenotyping-of-human-cd8-cytotoxic-t-cells-from-peripheral-blood-mononuclear-cells
#7
Tara Patel, Amy Cunningham, Martha Holland, John Daley, Suzan Lazo, F Stephen Hodi, Mariano Severgnini
The study of CD8 positive cells in peripheral blood has become an essential part of research in the field of cancer immunotherapies, vaccine development, inflammation, autoimmune disease, etc. In this study, an 8-color flow cytometry panel, containing lineage and functional markers, was developed for the identification of CD8+ cytotoxic T cells in previously cryopreserved peripheral blood mononuclear cells from healthy human donors. By studying functional markers in naïve and CD3/CD28 activated T cells we demonstrate that the panel is capable of detecting protein markers corresponding to different T cell activation statuses...
May 27, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28550492/microrna-889-is-downregulated-by-histone-deacetylase-inhibitors-and-confers-resistance-to-natural-killer-cytotoxicity-in-hepatocellular-carcinoma-cells
#8
Haitao Xie, Qiugui Zhang, Hui Zhou, Jun Zhou, Ji Zhang, Yan Jiang, Jinghong Wang, Xianglin Meng, Leping Zeng, Xiaoxin Jiang
Major histocompatibility complex class I chain-related gene B (MICB) is expressed on tumor cells and participates in natural killer (NK) cell-mediated antitumor immune response through engagement with the NKG2D receptor. This study was undertaken to identify novel microRNA (miRNA) regulators of MICB and clarify their functions in NK cell-mediated cytotoxicity to hepatocellular carcinoma (HCC) cells. Bioinformatic analysis and luciferase reporter assay were conducted to search for MICB-targeting miRNAs. Overexpression and knockdown experiments were performed to determine the roles of candidate miRNAs in the susceptibility of HCC cells to NK lysis...
May 26, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28540540/molecular-mechanisms-of-bombyx-batryticatus-ethanol-extract-inducing-gastric-cancer-sgc-7901-cells-apoptosis
#9
Jin-Yi Wu, Almutamad Sheikho, He Ma, Tian-Ci Li, Ya-Qi Zhao, Ya-Lin Zhang, Dun Wang
Bombyx batryticatus is a traditional Chinese medicine. To understand apoptotic effect of B. batryticatus ethanol extract (BBE), we investigated the role of BBE in inducing apoptosis of human gastric cancer cells SGC-7901. Cells treated with BBE and apoptosis was assessed by methyl thiazolyl tetrazolium (MTT) assay, morphological changes, DNA fragmentation and flow cytometry assays. The expression of Bcl-2, Bax and P21 were evaluated by western blot analysis and real time polymerase chain reaction. MTT assay showed that the cytotoxicity of BBE extract on SGC-7901 cells was correlated with treatment time and concentration...
May 24, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28536872/cytoprotective-propensity-of-green-tea-polyphenols-against-citrinin-induced-skeletal-myotube-damage-in-c2c12-cells
#10
G R Sharath Babu, N Ilaiyaraja, Farhath Khanum, T Anand
The mycotoxin citrinin, is produced by several species of Penicillium, Aspergillus and Monascus, and is capable of inducing cytotoxicity, oxidative stress and apoptosis. The aim of the present study was to investigate the effect of citrinin in mouse skeletal muscle cells (C2C12) and to overcome the cellular adverse effects by supplementing green tea extract (GTE) rich in polyphenols. C2C12 myoblasts were differentiated to myotubes and were exposed to citrinin in a dose dependent manner (0-100 µM) for 24 h and IC50 value was found to be 100 µM that resulted in decreased cell viability, increased LDH leakage and compromised membrane integrity...
May 23, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28536871/impact-of-early-subcultures-on-stemness-migration-and-angiogenic-potential-of-adipose-tissue-derived-stem-cells-and-their-resistance-to-in-vitro-ischemic-condition
#11
Hossein Faghih, Arash Javeri, Masoumeh Fakhr Taha
Adipose tissue-derived stem cells (ADSCs) are capable of multipotential differentiation and express several angiogenic, anti-apoptotic and immunomodulatory markers. These features make adipose tissue as a promising source of stem cells for regenerative medicine. However, for efficient translational use, culture-induced changes in the gene expression profile and resistance of the ADSCs to ischemic environment should be taken into consideration. We compared the expression of some clinically important markers between the unpassaged and third-passaged ADSCs by RT-PCR, qPCR and flow cytometry...
May 23, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28509991/camellia-sinensis-increased-apoptosis-on-u2os-osteosarcoma-cells-and-wound-healing-potential-on-nih3t3-fibroblast-cells
#12
Sinem Er, Miriş Dikmen
Camellia sinensis (Cs) is a plant which is rich in polyphenols and has antioxidant, antiinflammatory, antimutagenic, anticarcinogenic and antibacterial activities. In this study, two different methanol extracts (Cs-I and Cs-II) were prepared from the leaf of C. sinensis in order to investigate the wound healing and anticancer activities. Total phenolic content and antioxidant activity of the extracts were determined. Wound healing effects of Cs extracts were evaluated by using Masson's Trichrome Tecnique on NIH3T3 fibroblast cells...
May 16, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28497366/evaluation-of-persistence-and-distribution-of-intra-dermally-administered-pkh26-labelled-goat-bone-marrow-derived-mesenchymal-stem-cells-in-cutaneous-wound-healing-model
#13
M D Pratheesh, Nitin E Gade, Amar Nath, Pawan K Dubey, T B Sivanarayanan, D N Madhu, T R Sreekumar, Amarpal, G Saikumar, G Taru Sharma
The current study was designed to study the persistence and distribution of caprine bone marrow derived mesenchymal stem cells (cBM-MSCs) when administered intra-dermally in experimentally induced cutaneous wounds in rabbits. MSC's from goat bone marrow were isolated and their differentiation potential towards adipogenic and osteogenic lineages were assayed in vitro. The isolated cells were phenotypically analysed using flow cytometry for the expression of MSC specific matrix receptors (CD73, CD105 and Stro-1) and absence of hematopoietic lineage markers...
May 11, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28477232/cytogenetic-characterization-of-amaranthus-caudatus-l-and-amaranthus-hybridus-subsp-cruentus-l-thell
#14
V Prajitha, J E Thoppil
The present study is aimed to identify genetic variability between two species of Amaranthus viz., A. caudatus and A. hybridus subsp. cruentus, two economically important species, cultivated mainly for grain production. Karyomorphological studies in Amaranthus are scarce, probably due to higher number of small sized chromosomes. Karyomorphological studies were conducted using mitotic squash preparation of young healthy root tips. Karyological parameters and karyotypic formula were established using various software programs and tabulated the karyomorphometric and asymmetry indices viz...
May 5, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28474214/shell-extracts-of-the-edible-mussel-and-oyster-induce-an-enhancement-of-the-catabolic-pathway-of-human-skin-fibroblasts-in-vitro
#15
Thomas Latire, Florence Legendre, Mouloud Bouyoucef, Frédéric Marin, Franck Carreiras, Muriel Rigot-Jolivet, Jean-Marc Lebel, Philippe Galéra, Antoine Serpentini
Mollusc shells are composed of more than 95% calcium carbonate and less than 5% organic matrix consisting mostly of proteins, glycoproteins and polysaccharides. In this study, we investigated the effects of matrix macromolecular components extracted from the shells of two edible molluscs of economic interest, i.e., the blue mussel Mytilus edulis and the Pacific oyster Crassostrea gigas. The potential biological activities of these organic molecules were analysed on human dermal fibroblasts in primary culture...
May 4, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28470539/bovine-myoblast-cell-production-in-a-microcarriers-based-system
#16
Sanne Verbruggen, Daan Luining, Anon van Essen, Mark J Post
For several tissue engineering applications, in particular food products, scaling up culture of mammalian cells is a necessary task. The prevailing method for large scale cell culture is the stirred tank bioreactor where anchor dependent cells are grown on microcarriers suspended in medium. We use a spinner flask system with cells grown on microcarriers to optimize the growth of bovine myoblasts. Freshly isolated primary cells were seeded on microcarriers (Synthemax(®), CellBIND(®) and Cytodex(®) 1 MCs)...
May 3, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28466428/extracellular-vesicles-of-etv2-transfected-fibroblasts-stimulate-endothelial-cells-and-improve-neovascularization-in-a-murine-model-of-hindlimb-ischemia
#17
Phuc Van Pham, Ngoc Bich Vu, Thuy Thi-Thanh Dao, Ha Thi-Ngan Le, Lan Thi Phi, Oanh Thuy Huynh, Mai Thi-Hoang Truong, Oanh Thi-Kieu Nguyen, Ngoc Kim Phan
Ischemia are common conditions related to lack of blood supply to tissues. Depending on the ischemic sites, ischemia can cause different diseases, such as hindlimb ischemia, heart infarction and stroke. This study aims to evaluate how extracellular vesicles (EVs) derived from ETV2 transfected fibroblasts affect endothelial cell proliferation and neovascularization in a murine model of hindlimb ischemia. Human fibroblasts were isolated and cultured under standard conditions and expanded to the 3th passage before use in experiments...
May 2, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28421411/seleno-short-chain-chitosan-induces-apoptosis-in-human-non-small-cell-lung-cancer-a549-cells-through-ros-mediated-mitochondrial-pathway
#18
Yana Zhao, Shaojing Zhang, Pengfei Wang, Shengnan Fu, Di Wu, Anjun Liu
Seleno-short-chain chitosan (SSCC) is a synthesized chitosan derivative. In this study, antitumor activity and underlying mechanism of SSCC on human non-small-cell lung cancer A549 cells were investigated in vitro. The MTT assay showed that SSCC could inhibit cell viability in a dose- and time-dependent manner, and 200 μg/ml SSCC exhibited significantly toxic effects on A549 cells. The cell cycle assay showed that SSCC triggered S phase cell cycle arrest in a dose- and time-dependent manner, which was related to a downregulation of S phase associated cyclin A...
April 18, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28409292/effects-of-the-donor-age-on-proliferation-senescence-and-osteogenic-capacity-of-human-urine-derived-stem-cells
#19
Peng Gao, Peilin Han, Dapeng Jiang, Shulong Yang, Qingbo Cui, Zhaozhu Li
To study the effects of the donor age on the application potential of human urine-derived stem cells (hUSCs) in bone tissue engineering, by comparing proliferation, senescence and osteogenic differentiation of hUSCs originated from volunteers with different ages. The urine samples were collected from 19 healthy volunteers (6 cases from children group aged from 5 to 14, 5 cases from middle-aged group aged from 30 to 40, and 8 cases from the elder group aged from 65 to 75), and hUSCs were isolated and cultured...
April 13, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28397099/maturation-of-human-pluripotent-stem-cell-derived-cardiomyocytes-is-improved-in-cardiovascular-construct
#20
Hanna Vuorenpää, Kirsi Penttinen, Tuula Heinonen, Mari Pekkanen-Mattila, Jertta-Riina Sarkanen, Timo Ylikomi, Katriina Aalto-Setälä
In order to translate preclinical data into the clinical studies, relevant in vitro models with structure and key functional properties similar to native human tissue should be used. In vitro cardiac models with vascular structures mimic the highly vascularized myocardium and provide interactions between endothelial cells, stromal cells and cardiomyocytes. Currently, human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have been shown to present immature morphology and fetal-like electrophysiological properties that may limit their use as physiological test platform...
April 10, 2017: Cytotechnology
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