journal
MENU ▼
Read by QxMD icon Read
search

FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology

journal
https://www.readbyqxmd.com/read/30230922/signature-oriented-investigation-of-the-efficacy-of-multicomponent-drugs-against-heart-failure
#1
Junying Wei, Feifei Guo, Minyu Zhang, Minghua Xian, Tingting Wang, Jinhuan Gao, Hongwei Wu, Lei Song, Yi Zhang, Defeng Li, Hongjun Yang, Luqi Huang
Increasing attention has been paid to the application of precision medicine in heart failure (HF), and biomarker-based personalized medical care has shown great progress in HF management. Additionally, multicomponent drugs represented by traditional Chinese medicine (TCM) are demonstrating safety and efficacy in the management of HF. However, the potential mechanism is unclear, and the ambiguous clinical locations of TCM remain a hindrance to its extensive application. To meet this requirement, a precise investigation on the efficacy of multicomponent drugs against HF using the signature biomarkers-oriented approach was developed and applied to a conventional drug for cardiac disease, YiXinShu (YXS)...
September 19, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/30230921/calmodulin-regulates-mgrn1-gp78-interaction-mediated-ubiquitin-proteasomal-degradation-system
#2
Rukmini Mukherjee, Anshu Bhattacharya, Abhishek Sau, Samita Basu, Saikat Chakrabarti, Oishee Chakrabarti
The mechanism by which the endoplasmic reticulum (ER) ubiquitin ligases sense stress to potentiate their activity is poorly understood. GP78, an ER E3 ligase, is best known for its role in ER-associated protein degradation, although its activity is also linked to mitophagy, ER-mitochondria junctions, and MAPK signaling, thus highlighting the importance of understanding its regulation. In healthy cells, Mahogunin really interesting new gene (RING) finger 1 (MGRN1) interacts with GP78 and proteasomally degrades it to alleviate mitophagy...
September 19, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/30226813/linc-scrg1-accelerates-liver-fibrosis-by-decreasing-rna-binding-protein-tristetraprolin
#3
Jun-Cheng Wu, Sheng-Zheng Luo, Ting Liu, Lun-Gen Lu, Ming-Yi Xu
The biologic roles of long noncoding RNAs (lncRNAs) in liver fibrosis remained unknown. Through microarray analysis, linc-SCRG1 (a lncRNA with transcript length 3118 bp) was found up-regulated 13.62-fold in human cirrhotic tissues. Quantitative PCR verified that linc-SCRG1 increased along with liver fibrosis progression in human tissues and in activated LX2 cells induced by TGF-β1. Knock-down of linc-SCRG1 significantly reversed the effects of TGF-β1 on LX2, including inhibiting activation, promoting apoptosis, reducing proliferation, lessening invasion, and down-regulating genes [fibrosis-related mRNA: α-smooth muscle actin ( α-SMA), type Ι collagen, and B-cell lymphoma-2; invasion-related mRNA: matrix metallopeptidase-2 ( MMP-2), MMP-9, and MMP-13; inflammation-related mRNA: TNF-α, IL-6, and IL-10]...
September 18, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/30226812/insulin-induces-phosphorylation-of-pyruvate-dehydrogenase-through-rhoa-activation-pathway-in-hepg2-cells
#4
Rokibul Islam, Jae-Gyu Kim, Yohan Park, Jung-Yoon Cho, Kim-Cuong Cap, A-Ra Kho, Won-Suk Chung, Sang-Won Suh, Jae-Bong Park
Insulin is a critical signaling molecule in reducing blood glucose levels, and pyruvate dehydrogenase (PDH) is an essential enzyme in regulating glucose metabolism. However, the insulin effect on PDH function has not been well established. We observed that insulin attenuated the phosphorylation (p) of Ser264 (p-Ser264) in the PDH E1α subunit (PDHA1) in normal rat hepatocyte. In contrast, insulin induced an increase of p-Ser264 PDHA1 levels in hepatocellular carcinoma HepG2 and Huh7 cells. Insulin activated RhoA and Rho-dependent coiled coil kinase, an effector protein of active RhoA, which regulated p-Ser264 PDHA1 levels, along with both p-Ser9 and p-Tyr216 forms of glycogen synthase kinase-3β (GSK-3β) in HepG2 cells...
September 18, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/30226811/kinetics-of-corneal-leukocytes-by-intravital-multiphoton-microscopy
#5
Yashar Seyed-Razavi, Maria J Lopez, Dimosthenis Mantopoulos, Lixin Zheng, Steffen Massberg, Victor G Sendra, Deshea L Harris, Pedram Hamrah
Corneal immune privilege is integral in maintaining the clear avascular window to the foreign world. The presence of distinct populations of corneal leukocytes (CLs) in the normal cornea has been firmly established. However, their precise function and kinetics remain, as of yet, unclear. Through intravital multiphoton microscopy (IV-MPM), allowing the means to accumulate critical spatial and temporal cellular information, we provide details for long-term investigation of CL morphology and kinetics under steady state and following inflammation...
September 18, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/30226810/experimental-cerebral-malaria-is-associated-with-profound-loss-of-both-glycan-and-protein-components-of-the-endothelial-glycocalyx
#6
Casper Hempel, Jon Sporring, Jørgen Anders Lindholm Kurtzhals
Vascular pathology is central to malaria pathogenesis and associated with severity of disease. We have previously documented shedding of the cerebral endothelial glycocalyx in experimental malaria and hypothesized that this action is implicated in the pathogenesis of cerebral malaria (CM). Quantification and characterization of the intraluminal vascular glycocalyx are technically challenging. Here, we used ferritin labeling, computerized image analysis, and biochemical characterization by using in vivo biotinylation and pull down...
September 18, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/30226809/extracellular-vesicles-derived-from-human-umbilical-cord-mesenchymal-stem-cells-alleviate-rat-hepatic-ischemia-reperfusion-injury-by-suppressing-oxidative-stress-and-neutrophil-inflammatory-response
#7
Jia Yao, Jun Zheng, Jianye Cai, Kaining Zeng, Chaorong Zhou, Jiebin Zhang, Shihui Li, Hui Li, Liang Chen, Liying He, Huaxin Chen, Hongyuan Fu, Qi Zhang, Guihua Chen, Yang Yang, Yingcai Zhang
Mesenchymal stem cells (MSCs) have been reported to exert therapeutic effects on immunoregulation, tissue repair, and regeneration from the bench to the bedside. Increasing evidence demonstrates that extracellular vesicles (EVs) derived from MSCs could contribute to these effects and are considered as a potential replacement for stem cell-based therapies. However, the efficacy and underlying mechanisms of EV-based treatment in hepatic ischemia-reperfusion injury (IRI) remain unclear. Here, we demonstrated that human umbilical cord MSC-EVs (huc-MSC-EVs) could protect against IRI-induced hepatic apoptosis by reducing the infiltration of neutrophils and alleviating oxidative stress in hepatic tissue in vivo...
September 18, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/30226808/long-noncoding-rna-linc00261-induces-chemosensitization-to-5-fluorouracil-by-mediating-methylation-dependent-repression-of-dpyd-in-human-esophageal-cancer
#8
Kai Lin, Hong Jiang, Shan-Shan Zhuang, Yun-Sheng Qin, Guo-Dong Qiu, Yu-Qi She, Jie-Ting Zheng, Chen Chen, Ling Fang, Shu-Yao Zhang
Approximately 85% of a single administered dose of 5-fluorouracil (5-FU) will be degraded by dihydropyrimidine dehydrogenase (DYPD). Studies have highlighted a link between the complete or partial loss of DYPD function and clinical responses to 5-FU; however, the underlying molecular basis of DPD deficiency remains poorly understood. Hence, the aim of the present study was to evaluate the prevailing hypothesis which suggests that overexpression of LINC00261 possesses the ability to modulate the methylation-dependent repression of DPYD, ultimately resulting in an elevation of the sensitivity of human esophageal cancer cells to 5-FU...
September 18, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/30222079/ctrp13-inhibits-atherosclerosis-via-autophagy-lysosome-dependent-degradation-of-cd36
#9
Cheng Wang, Wenjing Xu, Minglu Liang, Dan Huang, Kai Huang
C1q/tumor necrosis factor-related protein 13 (CTRP13) is a secreted adipokine that can ameliorate abnormal glucose and lipid metabolism. However, the functional role of CTRP13 in the development of atherosclerotic plaques has yet to be described. In this study, we collected blood samples from patients of coronary artery diseases and apolipoprotein E (ApoE)-/- mice that were fed a Western diet for 12 wk to induce atherosclerosis and found that serum CTRP13 level was decreased. En face staining of aortas and aortic sinus in ApoE-/- mice showed that ectopic CTRP13 infusion in vivo dramatically decreased lesion areas, as well as reduced inflammatory responses with less macrophage content...
September 17, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/30222078/tissue-specific-regulation-of-cytochrome-c-by-post-translational-modifications-respiration-the-mitochondrial-membrane-potential-ros-and-apoptosis
#10
Hasini A Kalpage, Viktoriia Bazylianska, Maurice A Recanati, Alemu Fite, Jenney Liu, Junmei Wan, Nikhil Mantena, Moh H Malek, Izabela Podgorski, Elizabeth I Heath, Asmita Vaishnav, Brian F Edwards, Lawrence I Grossman, Thomas H Sanderson, Icksoo Lee, Maik Hüttemann
Cytochrome c (Cyt c) plays a vital role in the mitochondrial electron transport chain (ETC). In addition, it is a key regulator of apoptosis. Cyt c has multiple other functions including ROS production and scavenging, cardiolipin peroxidation, and mitochondrial protein import. Cyt c is tightly regulated by allosteric mechanisms, tissue-specific isoforms, and post-translational modifications (PTMs). Distinct residues of Cyt c are modified by PTMs, primarily phosphorylations, in a highly tissue-specific manner...
September 17, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/30222077/abcb1-p-glycoprotein-regulates-vitamin-d-absorption-and-contributes-to-its-transintestinal-efflux
#11
Marielle Margier, Xavier Collet, Cédric le May, Charles Desmarchelier, François André, Chantal Lebrun, Catherine Defoort, Alice Bluteau, Patrick Borel, Anne Lespine, Emmanuelle Reboul
Efficient intestinal absorption of dietary vitamin D is required in most people to ensure an adequate status. Thus, we investigated the involvement of ATP binding cassette subfamily B member 1 (ABCB1) in vitamin D intestinal efflux. Both cholecalciferol (D3 ) and 25-hydroxycholecalciferol [25(OH)D3 ] apical effluxes were decreased by chemical inhibition of ABCB1 in Caco-2 cells and increased by ABCB1 overexpression in Griptites or Madin-Darby canine kidney type II cells. Mice deficient for the 2 murine ABCB1s encoded by Abcb1a and Abcb1b genes ( Abcb1-/- ) displayed an accumulation of 25(OH)D3 in plasma, intestine, brain, liver, and kidneys, together with an increased D3 postprandial response after gavage compared with controls...
September 17, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/30216112/microrna-351-5p-mediates-skeletal-myogenesis-by-directly-targeting-lactamase-%C3%AE-and-is-regulated-by-lnc-mg
#12
Jingjing Du, Peiwen Zhang, Xue Zhao, Jin He, Yan Xu, Qin Zou, Jia Luo, Linyuan Shen, Hao Gu, Qianzi Tang, Mingzhou Li, Yanzhi Jiang, Guoqing Tang, Lin Bai, Xuewei Li, Jinyong Wang, Shunhua Zhang, Li Zhu
Skeletal muscle is an important and complex organ with a variety of functions in humans and animals. Skeletal myogenesis is a multistep and complex process, and increasing evidence suggests that microRNAs (miRNAs) and long noncoding RNAs (lncRNAs) play critical roles in skeletal myogenesis. In this study the expression of miR-351-5p is dynamically regulated during skeletal myogenesis in vitro and in vivo. Cell-counting kit-8, qRT-PCR, and EdU immunofluorescence analysis showed that miR-351-5p overexpression promoted the proliferation and inhibited the differentiation of C2C12 myoblast, whereas inhibition of miR-351-5p had the opposite effect...
September 14, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/30216111/%C3%AE-arrestin1-enhances-liver-fibrosis-through-autophagy-mediated-snail-signaling
#13
Siwei Tan, Yu Lu, Minyi Xu, Xiaoli Huang, Huiling Liu, Jie Jiang, Bin Wu
GPCR mediator β-arrestins (β-arr1 and β-arr2) regulate a variety of physiologic and pathologic processes, including hepatocellular carcinogenesis. However, the role of β-arrestins in liver fibrosis remains unknown. β-arr1, but not β-arr2, was upregulated in liver fibrotic tissues in both humans and mice; moreover, autophagy was increased. β-arr1 deficiency or autophagic inhibitor 3-methyladenine (3-MA) significantly blocked autophagy and downregulated liver fibrosis. Furthermore, β-arr1 enhanced hepatocyte compensatory proliferation, and hepatic stellate cell growth with activation via autophagy resulted in liver fibrosis...
September 14, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/30216110/skullcapflavone-ii-inhibits-osteoclastogenesis-by-regulating-reactive-oxygen-species-and-attenuates-the-survival-and-resorption-function-of-osteoclasts-by-modulating-integrin-signaling
#14
Jiae Lee, Han Saem Son, Hye In Lee, Gong-Rak Lee, You-Jin Jo, Seong-Eun Hong, Narae Kim, Minjeong Kwon, Nam Young Kim, Hyun Jin Kim, Yoo Jin Lee, Eun Kyoung Seo, Woojin Jeong
Many bone diseases, such as osteoporosis and rheumatoid arthritis, are attributed to an increase in osteoclast number or activity; therefore, control of osteoclasts has significant clinical implications. This study shows how skullcapflavone II (SFII), a flavonoid with anti-inflammatory activity, regulates osteoclast differentiation, survival, and function. SFII inhibited osteoclastogenesis with decreased activation of MAPKs, Src, and cAMP response element-binding protein (CREB), which have been known to be redox sensitive...
September 14, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/30216109/genetic-manipulation-of-ccn2-ctgf-unveils-cell-specific-ecm-remodeling-effects-in-injured-skeletal-muscle
#15
Jennifer M Petrosino, Andrew Leask, Federica Accornero
In skeletal muscle, extracellular matrix (ECM) remodeling can either support the complete regeneration of injured muscle or facilitate pathologic fibrosis and muscle degeneration. Muscular dystrophy (MD) is a group of genetic disorders that results in a progressive decline in muscle function and is characterized by the abundant deposition of fibrotic tissue. Unlike acute injury, where ECM remodeling is acute and transient, in MD, remodeling persists until fibrosis obstructs the regenerative efforts of diseased muscles...
September 14, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/30211660/mesencephalic-astrocyte-derived-neurotrophic-factor-affords-neuroprotection-to-early-brain-injury-induced-by-subarachnoid-hemorrhage-via-activating-akt-dependent-prosurvival-pathway-and-defending-blood-brain-barrier-integrity
#16
Tao Li, Weilin Xu, Liansheng Gao, Guoping Guan, Zhongyuan Zhang, Pingyou He, Hangzhe Xu, Linfeng Fan, Feng Yan, Gao Chen
This study aimed to explore the neuroprotective effect of mesencephalic astrocyte-derived neurotrophic factor (MANF) protein on early brain injury caused by subarachnoid hemorrhage (SAH) and the relevant mechanisms in experimental rats, expecting to understand whether MANF was a potential therapeutic target for SAH treatment. A perforation model of SAH was introduced into the study. Recombinant human MANF (rh-MANF) and protein kinase B (Akt) inhibitor (MK2206) were used to explore the effect and the mechanisms...
September 13, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/30211659/the-lipid-environment-determines-the-activity-of-the-escherichia-coli-ammonium-transporter-amtb
#17
Gaëtan Dias Mirandela, Giulia Tamburrino, Paul A Hoskisson, Ulrich Zachariae, Arnaud Javelle
The movement of ammonium across biologic membranes is a fundamental process in all living organisms and is mediated by the ubiquitous ammonium transporter/methylammonium permease/rhesus protein (Amt/Mep/Rh) family of transporters. Recent structural analysis and coupled mass spectrometry studies have shown that the Escherichia coli ammonium transporter AmtB specifically binds 1-palmitoyl-2-oleoyl phosphatidylglycerol (POPG). Upon POPG binding, several residues of AmtB undergo a small conformational change, which stabilizes the protein against unfolding...
September 13, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/30207799/substrate-induced-conformational-change-in-cytochrome-p450-olep
#18
Giacomo Parisi, Linda Celeste Montemiglio, Alessandro Giuffrè, Alberto Macone, Antonella Scaglione, Gabriele Cerutti, Cécile Exertier, Carmelinda Savino, Beatrice Vallone
The regulation of cytochrome P450 activity is often achieved by structural transitions induced by substrate binding. We describe the conformational transition experienced upon binding by the P450 OleP, an epoxygenase involved in oleandomycin biosynthesis. OleP bound to the substrate analog 6DEB crystallized in 2 forms: one with an ensemble of open and closed conformations in the asymmetric unit and another with only the closed conformation. Characterization of OleP-6DEB binding kinetics, also using the P450 inhibitor clotrimazole, unveiled a complex binding mechanism that involves slow conformational rearrangement with the accumulation of a spectroscopically detectable intermediate where 6DEB is bound to open OleP...
September 12, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/30207798/iron-overload-by-transferrin-receptor-protein-1-regulation-plays-an-important-role-in-palmitate-induced-insulin-resistance-in-human-skeletal-muscle-cells
#19
Rihua Cui, Sung-E Choi, Tae Ho Kim, Hwa Joung Lee, Soo Jin Lee, Yup Kang, Ja Young Jeon, Hae Jin Kim, Kwan-Woo Lee
Free fatty acid is considered to be one of the major pathogenic factors of inducing insulin resistance. The association between iron disturbances and insulin resistance has recently begun to receive a lot of attention. Although skeletal muscles are a major tissue for iron utilization and storage, the role of iron in palmitate (PA)-induced insulin resistance is unknown. We investigated the molecular mechanism underlying iron dysregulation in PA-induced insulin resistance. Interestingly, we found that PA simultaneously increased intracellular iron and induced insulin resistance...
September 12, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/30207797/cd36-triggered-cell-invasion-and-persistent-tissue-colonization-by-tumor-microvesicles-during-metastasis
#20
Susanne Pfeiler, Manovriti Thakur, Petra Grünauer, Remco T A Megens, Urjita Joshi, Raffaele Coletti, Verena Samara, Geraldine Müller-Stoy, Hellen Ishikawa-Ankerhold, Konstantin Stark, Andreas Klingl, Thomas Fröhlich, Georg J Arnold, Sonja Wörmann, Christiane J Bruns, Hana Algül, Christian Weber, Steffen Massberg, Bernd Engelmann
Tumor microvesicles are a peculiar type of extracellular vesicles that circulate in the blood of patients with metastatic cancer. The itineraries and immune cell interactions of tumor microvesicles during the intravascular and extravascular stages of metastasis are largely unknown. We found that the lipid receptor CD36 is a major mediator of the engulfment of pancreatic tumor microvesicles by myeloid immune cells in vitro and critically samples circulating tumor microvesicles by resident liver macrophages in mice in vivo...
September 12, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
journal
journal
29811
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"