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European Journal of Clinical Microbiology & Infectious Diseases

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https://www.readbyqxmd.com/read/29909468/a-high-incidence-and-coexistence-of-multiresistance-genes-cfr-and-optra-among-linezolid-resistant-enterococci-isolated-from-a-teaching-hospital-in-wenzhou-china
#1
Yizhi Zhang, Guofeng Dong, Jiahui Li, Lijiang Chen, Haiyang Liu, Wenzi Bi, Hong Lu, Tieli Zhou
Linezolid is considered as a last-resort antimicrobial agent, the resistance of which is of great concern. The aim of this study was to investigate the mechanisms and transferability of linezolid resistance and molecular epidemiology of linezolid-resistant enterococcal isolates in Wenzhou, China. A collection of 1623 enterococcal strains, including 789 Enterococcus faecalis and 834 Enterococcus faecium, were isolated from our hospital during 2011-2016. Antimicrobial susceptibility testing and clinical data analysis were performed...
June 16, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29882176/how-to-achieve-accurate-blood-culture-volumes-the-bd-bactec-fx-blood-volume-monitoring-system-as-a-measuring-instrument-and-educational-tool
#2
L Cattoir, J Claessens, R Cartuyvels, A M Van den Abeele
Collecting adequate volumes of blood in blood culture bottles is crucial for sensitive detection of bacteremia and fungemia. Tools enabling easy collection of data on the degree of blood culture bottle filling at different hospital departments are an important step toward quality measurement and improvement. In this study, we verified the accuracy of a software tool for the monitoring of blood culture bottle filling developed by Becton Dickinson, BD blood volume monitoring system (BVMS) that was adjusted for use on plastic BACTEC bottles, and evaluated its ease of use in routine practice...
June 7, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29882175/antimicrobial-resistance-and-molecular-epidemiology-using-whole-genome-sequencing-of-neisseria-gonorrhoeae-in-ireland-2014-2016-focus-on-extended-spectrum-cephalosporins-and-azithromycin
#3
L Ryan, D Golparian, N Fennelly, L Rose, P Walsh, B Lawlor, M Mac Aogáin, M Unemo, B Crowley
High-level resistance and treatment failures with ceftriaxone and azithromycin, the first-line agents for gonorrhoea treatment are reported and antimicrobial-resistant Neisseria gonorrhoeae is an urgent public health threat. Our aims were to determine antimicrobial resistance rates, resistance determinants and phylogeny of N. gonorrhoeae in Ireland, 2014-2016. Overall, 609 isolates from four University Hospitals were tested for susceptibility to extended-spectrum cephalosporins (ESCs) and azithromycin by the MIC Test Strips...
June 7, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29876774/rapid-antigen-test-use-for-the-management-of-group-a-streptococcal-pharyngitis-in-community-pharmacies
#4
Béatrice Demoré, Gianpiero Tebano, Julien Gravoulet, Christophe Wilcke, Eric Ruspini, Jacques Birgé, Jean-Marc Boivin, Sandrine Hénard, Annick Dieterling, Lidiana Munerol, Julie Husson, Christian Rabaud, Céline Pulcini, Sophie Malblanc
Despite group A streptococci being an infrequent cause of pharyngitis in adult outpatients, sore throat remains a common indication for antibiotic prescription. This prospective multicentre non-randomised study describes a community pharmacy-based antimicrobial stewardship intervention consisting in the implementation of rapid antigen testing (RAT) for the management of adults with sore throat. Trained pharmacists triaged patients presenting with symptoms of pharyngitis using the modified Centor score. Those at risk for streptococcal infection were tested with RAT...
June 6, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29876773/comparative-activity-of-ceftobiprole-against-coagulase-negative-staphylococci-from-the-bsac-bacteraemia-surveillance-programme-2013-2015
#5
Anne Santerre Henriksen, Jennifer Smart, Kamal Hamed
Coagulase-negative staphylococci (CoNS) are a significant cause of bacteraemia, the treatment of which is becoming increasingly complex due to the emergence of multidrug-resistant strains. This study aimed to evaluate the in vitro activity of ceftobiprole, an advanced-generation cephalosporin, as compared with other antimicrobial agents against CoNS from patients with bacteraemia. As part of the British Society for Antimicrobial Chemotherapy (BSAC) Bacteraemia Surveillance Programme, 650 blood isolates of CoNS were obtained from patients with bacteraemia at 74 centres throughout the UK and Ireland for the years 2013-2015...
June 6, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29876772/attitudes-of-physicians-from-10-european-countries-on-adherence-and-how-treatment-modalities-in-absssi-affect-adherence-results-from-a-delphi-survey
#6
Tom Stargardt, Christian Eckmann, Emilio Bouza, Gian Maria Rossolini, Gian Maria Grossi
To explore the attitudes of European physicians on adherence and how treatment modalities impact adherence in complicated forms of soft skin and skin structure infections, now referred as acute bacterial skin and skin structures infections (ABSSSI). After literature review, a questionnaire was prepared. Topics focused on (1) the importance of adherence, (2) the importance of administration regimen on adherence, (3) the importance of drug selection on adherence, (4) the importance of complexity on choice of drug for treatment, (5) the role of adherence in drug resistance, and (6) the role of adherence in administration of long-acting antibiotics (ABs)...
June 6, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29876771/human-respiratory-syncytial-virus-pathogenesis-immune-responses-and-current-vaccine-approaches
#7
REVIEW
Sara A Taleb, Asmaa A Al Thani, Khalid Al Ansari, Hadi M Yassine
Respiratory syncytial virus continues to pose a serious threat to the pediatric populations worldwide. With a genomic makeup of 15,200 nucleotides, the virus encodes for 11 proteins serving as envelope spikes, inner envelope proteins, and non-structural and ribonucleocapsid complexes. The fusion (F) and attachment (G) surface glycoproteins are the key targets for neutralizing antibodies. The highly variable G with altered glycosylations and the conformational alternations of F create challenges for vaccine development...
June 6, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29862422/hiv-serology-signal-to-cutoff-ratio-as-a-rapid-method-to-predict-confirmation-of-hiv-infection
#8
Lisa Li, David Puddicombe, Sylvie Champagne, Agatha Jassem, Mel Krajden, Linda Merrick, Christopher Lowe, Michael Payne
Early and rapid detection of patients with HIV is a key to preventing further transmission. The purpose of this study was to assess the ability of signal-to-cutoff (S/CO) ratio from initial screening fourth-generation HIV serology to predict subsequent confirmation of HIV. Patients with a first-time positive HIV serology (S/CO ratio ≥ 1) from 2012 to 2016 were included. Ratios were compared to the results of confirmatory testing. Predictive probabilities (PPs) of a positive confirmatory result were calculated based on a logistic regression model...
June 3, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29862421/a-new-kit-to-detect-campylobacter-species-in-stool-specimens-the-orion-genread-campylobacter%C3%A2
#9
Alice Buissonnière, Lucie Bénéjat, Paul Charron, Emilie Bessède, Philippe Lehours, Guillaume Valdenaire, Olivier Richer, Francis Mégraud
Campylobacter enteritis is the most frequent bacterial enteritis including in children. Its diagnosis suffers from the lack of sensitivity and delayed result of culture. Our aim was to test a new PCR-derived method for Campylobacter diagnosis in comparison to a composite reference. Patients presenting to the emergency ward of our hospital with enteric symptoms during the 2016 summer season were included. In addition to culture, an ELISA and an in-house real-time PCR were performed, as well as the new method (Orion GenRead Campylobacter) on all stool specimens...
June 3, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29855842/implementing-a-hospital-wide-protocol-for-staphylococcus-aureus-bacteremia
#10
K Bolhuis, L J Bakker, J T Keijer, P J de Vries
Staphylococcus aureus bacteraemia (SAB) is associated with high-mortality and complication rates. A multidisciplinary approach is needed to predict, detect and treat complications. In this pre- and post-intervention study, we investigated the effects of a hospital-wide protocol for diagnosis, classification and treatment of SAB. It was hypothesized that complications and endocarditis would be better identified and treated. Medical records of SAB patients admitted in 2011 and 2012 (pre) were analysed. In 2013, a protocol, describing risk factors, diagnostic classification and recommended treatment, was implemented...
May 31, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29855841/correction-to-prediction-of-unfavorable-outcomes-in-cryptococcal-meningitis-results-of-the-multicenter-infectious-diseases-international-research-initiative-id-iri-cryptococcal-meningitis-study
#11
I N Hakyemez, H Erdem, G Beraud, M Lurdes, A Silva-Pinto, C Alexandru, B Bishop, F Mangani, X Argemi, M Poinot, R Hasbun, M Sunbul, M Akcaer, S Alp, T Demirdal, K Angamuthu, F Amer, E Ragab, G A Shehata, D Ozturk-Engin, N Ozgunes, L Larsen, S Zimmerli, O R Sipahi, E Tukenmez Tigen, G Celebi, N Oztoprak, A C Yardimci, Y Cag
In the original version of this article, Mustafa Sunbul was not included in the list of authors for this article. The name has been added accordingly.
May 31, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29808350/evaluation-of-the-accelerate-pheno%C3%A2-system-for-rapid-identification-and-antimicrobial-susceptibility-testing-of-gram-negative-bacteria-in-bloodstream-infections
#12
Ghislaine Descours, Laurent Desmurs, Thi Lam Thuy Hoang, Marine Ibranosyan, Maud Baume, Anne-Gaëlle Ranc, Christine Fuhrmann, Olivier Dauwalder, Waël Salka, François Vandenesch
Identification and antimicrobial susceptibility testing (AST) are critical steps in the management of bloodstream infections. Our objective was to evaluate the performance of the Accelerate Pheno™ System, CE v1.2 software, for identification and AST of Gram-negative pathogens from positive blood culture bottles. A total of 104 bottles positive for Gram-negative bacteria collected from inpatients throughout our institution were randomly selected after Gram staining. The time-to-identification and AST results, and the raw AST results obtained by the Accelerate Pheno™ system and routine techniques (MALDI-TOF MS and VITEK®2, EUCAST guidelines) were compared...
May 28, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29804281/interferon-%C3%AE-and-cxcl10-responses-related-to-complaints-in-patients-with-q-fever-fatigue-syndrome
#13
Ruud P H Raijmakers, Anne F M Jansen, Stephan P Keijmel, Teske Schoffelen, Anja Scholzen, Jos W M van der Meer, Leo A B Joosten, Mihai G Netea, Marcel van Deuren, Chantal P Bleeker-Rovers
Approximately 20% of patients with acute Q fever develop Q fever fatigue syndrome (QFS), a debilitating fatigue syndrome. This study further investigates the role of C. burnetii-specific IFNγ, but also IL-2, CXCL9, CXCL10, and CXLC11 production in QFS patients. C. burnetii-specific IFNy, IL-2, CXCL9, CXCL10, and CXCL11 production were tested in ex vivo stimulated whole blood of QFS patients who recovered from their complaints (n = 8), QFS patients with persisting complaints (n = 27), and asymptomatic Q fever seropositive controls (n = 10)...
May 26, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29802481/management-of-chickenpox-in-pregnant-women-an-italian-perspective
#14
REVIEW
Serena Parente, Nicola Schiano Moriello, Alberto Enrico Maraolo, Grazia Tosone
Chickenpox is a highly contagious disease caused by primary infection of varicella zoster virus (VZV). The disease is spread worldwide and is usually benign but, in some groups of population like pregnant women, can have a severe outcome. Due to a not optimal vaccination coverage, a relatively high number of childbearing-aged women in a European country such as Italy tested seronegative for VZV and so are currently at risk of acquiring chickenpox during pregnancy, especially if they live in contact with children for family or work reasons...
May 25, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29797096/correction-to-antimicrobial-coated-sutures-to-decrease-surgical-site-infections-a-systematic-review-and-meta-analysis
#15
X Wu, N Z Kubilay, J Ren, B Allegranzi, P Bischoff, B Zayed, D Pittet, J Li
In the original version of this article, reference citations found in Tables 2, 3, and 4 contain errors in linking. The correct tables are reproduced below.
May 24, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29796984/subsequent-infection-with-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae-in-patients-with-prior-infection-or-fecal-colonization
#16
Anna Lindblom, Nahid Karami, Tim Magnusson, Christina Åhrén
In clinical practice, there is a growing need to assess the impact of prior colonization or infection with extended-spectrum β-lactamase-producing Enterobacteriaceae (EPE) on new EPE infections. We have investigated the frequency of, and duration to, a subsequent EPE infection in patients with prior fecal carriage or infection with EPE. Culture data for 3272 EPE-positive patients in Western Sweden during 2004-2014 were evaluated. The median follow-up time was 3.7 years. The first recorded EPE-positive fecal screen, or clinical (urine, blood) culture, and subsequent EPE-positive clinical samples were analyzed, focusing on the first and last recurrence of EPE infection...
May 23, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29785622/retrospective-surveillance-of-antibiotic-use-in-maternity-wards-and-neonatal-intensive-care-units-in-saint-petersburg-russia
#17
Timofey L Galankin, Alexey S Kolbin, Sergey V Sidorenko, Alexey A Kurylev, Elena A Malikova, Yuri V Lobzin, Dmitry O Ivanov, Nikolay P Shabalov, Anton V Mikhailov, Nikolay N Klimko, Gennadiy V Dolgov
Antibiotic overuse in infants is associated with an increased risk of serious adverse events. Development of antibiotic stewardship programs aimed at reducing overall antibiotic consumption requires epidemiological surveillance. Retrospective surveillance and evaluation of all antibiotics provided to every infant admitted to maternal wards or neonatal intensive care units (NICUs) from 01 January 2014 to 31 December 2014 were performed in five medical centers of Saint Petersburg, Russia. Types of antibiotics and dates of administration were recorded...
May 21, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29777490/comparison-of-the-etiological-relevance-of-staphylococcus-haemolyticus-and-staphylococcus-hominis
#18
Hagen Frickmann, Andreas Hahn, Romy Skusa, Nils Mund, Vivian Viehweger, Thomas Köller, Kerstin Köller, Norbert Georg Schwarz, Karsten Becker, Philipp Warnke, Andreas Podbielski
The study was performed to assess potential differences in the etiological relevance of two coagulase-negative staphylococci (CoNS), Staphylococcus haemolyticus and Staphylococcus hominis, in an observational single-center study. Over a 5-year interval, patients in whom there was detected S. haemolyticus or S. hominis of presumed etiological relevance were assessed for the primary endpoint death during hospital stay and the secondary endpoint transfer to an intensive care unit (ICU) after the detection of S...
May 19, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29777489/serum-antibody-response-to-chlamydia-trachomatis-troa-and-htra-in-women-with-tubal-factor-infertility
#19
T Rantsi, P Joki-Korpela, K Hokynar, I Kalliala, H Öhman, H-M Surcel, J Paavonen, A Tiitinen, M Puolakkainen
Persistent genital chlamydial infection may lead to tubal factor infertility (TFI). Chlamydia trachomatis TroA and HtrA are proteins expressed during persistent chlamydial infection in vitro. We studied serum IgG antibody response against these proteins by EIA in women with TFI and in subfertile women without tubal pathology. Altogether, 22 of 258 subfertile women (8.5%) had TFI which was unilateral in 17 cases and bilateral in 5 cases. Overall, 55 (21.3%) of the 258 women had TroA and 39 (15.1%) had HtrA antibodies...
May 18, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29777488/no-need-for-a-urine-culture-in-elderly-hospitalized-patients-with-a-negative-dipstick-test-result
#20
Zvi Shimoni, Vered Hermush, Joseph Glick, Paul Froom
To determine the clinical utility/disutility of canceling urine cultures in elderly patients with a negative dipstick. The cohort included consecutive patients aged ≥ 65 years hospitalized in internal medicine departments with an admission urinalysis and urine culture (January 1, 2014 to December 31, 2016). We calculated the sensitivity of the dipstick (either a trace leukocyte esterase or a positive nitrite test result) to detect patients with bacteriuria, and the decrease in urine cultures resulting from cancelation in patients with a negative dipstick...
May 18, 2018: European Journal of Clinical Microbiology & Infectious Diseases
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