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European Journal of Clinical Microbiology & Infectious Diseases

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https://www.readbyqxmd.com/read/29450768/cc398-staphylococcus-aureus-subpopulations-in-belgian-patients
#1
M Angeles Argudín, A Deplano, S Vandendriessche, M Dodémont, C Nonhoff, O Denis, S Roisin
Studies based on genome-wide single nucleotide polymorphisms (SNPs) supported the existence of two subpopulations in clonal complex (CC) 398 Staphylococcus aureus: an ancestral human-adapted clade (HC) and an animal-associated clade (AC). In this study, we have investigated the occurrence of genetic markers that allow discrimination of these subpopulations among CC398 isolates collected during 2014 to 2016 from human patients in Belgium. A collection of isolates was investigated by means of spa-typing and 16S-mecA-nuc PCR...
February 15, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29450767/epididymo-orchitis-caused-by-enteric-organisms-in-men-35-years-old-beyond-fluoroquinolones
#2
REVIEW
Laura Ryan, Padraig Daly, Ivor Cullen, Maeve Doyle
Epididymo-orchitis is a common urological condition in men of all ages, causing a unilateral or bilateral swelling of the epididymis and/or testis. It is frequently caused by sexually transmitted infections, Chlamydia trachomatis and Neisseria gonorrheae, as well as common enteric organisms implicated in urinary tract infections. Men over 35 years old may develop epididymo-orchitis associated with enteric organisms, often associated with functional bladder outlet problems such as benign prostatic hyperplasia or urethral stricture disease...
February 15, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29442195/the-changing-epidemiology-of-vanb-enterococcus-faecium-in-poland
#3
Ewa Sadowy, Iwona Gawryszewska, Alicja Kuch, Dorota Żabicka, Waleria Hryniewicz
Increasing prevalence of VanB Enterococcus faecium in Polish hospitals reported to National Reference Centre for Susceptibility Testing (NRCST) prompted us to investigate the basis of this phenomenon. Two-hundred seventy-eight E. faecium isolates of VanB phenotype from the period 1999 to 2010 obtained by NRCST were investigated by multilocus sequence typing (MLST) and multilocus VNTR analysis (MLVA). Localization, transferability, and partial structure of the vanB-carrying Tn1549 transposon were studied by hybridization, PCR mapping, sequencing, and conjugation...
February 13, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29428976/spa-type-distribution-in-mrsa-and-mssa-bacteremias-and-association-of-spa-clonal-complexes-with-the-clinical-characteristics-of-bacteremia
#4
Elina Jokinen, Laura Lindholm, Reetta Huttunen, Heini Huhtala, Risto Vuento, Jaana Vuopio, Jaana Syrjänen
The genetic distribution of invasive methicillin-susceptible (MSSA) and resistant S. aureus (MRSA) strains has to be addressed in order to target infection control strategies. A large MRSA epidemic caused by a certain MRSA strain (spa type 067) broke out in 2001 in our health district. We wanted to investigate the current spa type distribution in MRSA and MSSA bacteremias and assess the potential association of spa clonal complexes (spaCC) with the clinical characteristics of S. aureus bacteremia. One hundred nine invasive MRSA isolates and 353 invasive MSSA isolates were spa typed and grouped into clonal complexes (spaCC)...
February 10, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29423619/smoking-is-not-associated-with-higher-prevalence-of-jc-virus-in-ms-patients
#5
Michael Auer, Gabriel Bsteh, Harald Hegen, Franziska Di Pauli, Sebastian Wurth, Thomas Berger, Florian Deisenhammer
John Cunningham virus (JCV) causes rare, but potentially life-threatening progressive multifocal leukoencephalopathy (PML) in natalizumab-treated multiple sclerosis (MS) patients. Beside JCV index, there is currently no other factor for further risk stratification. Because smoking was reported as potential risk factor for several viral and bacterial infections, we aimed to investigate whether smoking could increase the risk for JCV infection in MS patients. We screened our database of the MS Clinic of the Department of Neurology, Medical University of Innsbruck, Austria, for patients with known smoking status and test result for anti-JCV antibody index as determined by two-step ELISA at Unilabs, Copenhagen, Denmark...
February 8, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29417312/lessons-learned-from-splenic-infarcts-with-fever-of-unknown-origin-fuo-culture-negative-endocarditis-cne-or-malignancy
#6
REVIEW
Burke A Cunha, Bertamaria Dieguez, Alena Varantsova
Culture negative endocarditis (CNE) is a common concern in patients with fever, heart murmur, cardiac vegetation, and negative blood cultures. The diagnosis of CNE is not based only on negative blood cultures and a cardiac vegetation. The clinical definition of CNE is based on negative blood cultures plus the findings of culture positive infective endocarditis (IE), e.g., fever, cardiac vegetation, splenomegaly, peripheral manifestations. Because embolic splenic infarcts may occur with culture positive IE, some may assume that splenic infarcts are a sign of CNE...
February 7, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29417311/genotypic-diversity-of-streptococcus-suis-strains-isolated-from-humans-in-thailand
#7
Anusak Kerdsin, Yukihiro Akeda, Dan Takeuchi, Surang Dejsirilert, Marcelo Gottschalk, Kazunori Oishi
The purpose of this study is to characterize Streptococcus suis isolates recovered from human infections regarding serotype distribution, genotypic profile, clinical manifestations, and epidemiology. A total of 668 S. suis isolates recovered from human infections in Thailand were characterized based on serotyping by multiplex PCR and co-agglutination, genotypic profiles by multilocus sequence typing, and PCR for virulence-associated genes, as well as review of medical records. Serotype 2 (94.6%) was predominant, followed by serotype 14 (4...
February 7, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29417310/human-papillomavirus-genotype-and-viral-load-agreement-between-paired-first-void-urine-and-clinician-collected-cervical-samples
#8
Severien Van Keer, Wiebren A A Tjalma, Jade Pattyn, Samantha Biesmans, Zoë Pieters, Xaveer Van Ostade, Margareta Ieven, Pierre Van Damme, Alex Vorsters
The performance and acceptability of first-void urine as specimen for the detection of HPV DNA in a Belgian referral population was evaluated using an optimized sample collection and processing protocol. One hundred ten first-void urine and cervical samples were collected from 25- to 64-year-old women who were referred for colposcopy (January-November 2016). Paired samples were analyzed by the Riatol qPCR HPV genotyping assay. Acceptability data were gathered through questionnaires (NCT02714127). A higher high-risk HPV DNA prevalence was observed in first-void urine (n = 76/110) compared to cervical samples (n = 73/110), with HPV31 and HPV16/31 being most prevalent correspondingly...
February 7, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29411191/diagnostic-challenges-in-pyogenic-spinal-infection-an-expanded-role-for-fdg-pet-ct
#9
Gannon J Yu, Ingrid L Koslowsky, Silvia A Riccio, Angel K M Chu, Harvey R Rabin, Reinhard Kloiber
In a preliminary investigation of FDG-PET/CT for assessment of therapy response of pyogenic spine infection, it was concluded that activity confined to the margins of a destroyed or degenerated joint with bone-on-bone contact represents nonseptic inflammation, regardless of the intensity of uptake. Only activity in bone, soft tissue, or within the epidural space represents active infection. The purpose of this investigation was to assess the performance of these pattern-based interpretation criteria in a series of problem cases of proven or suspected spine infection...
February 6, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29411190/hand-foot-and-mouth-disease-current-knowledge-on-clinical-manifestations-epidemiology-aetiology-and-prevention
#10
REVIEW
Susanna Esposito, Nicola Principi
For a long time, hand, foot and mouth disease (HFMD) was seen as a mild viral infection characterized by typical clinical manifestations that spontaneously resolved in a few days without complications. In the past two decades, HFMD has received new attention because of evidence that this disease could have clinical, epidemiological and aetiological characteristics quite different from those initially thought. In contrast to previous beliefs, it has been clarified that HFMD can be associated with complications, leading to severe neurological sequelae and, rarely, to death...
February 6, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29411189/guillain-barr%C3%A3-syndrome-following-varicella-zoster-virus-infection
#11
Badrul Islam, Zhahirul Islam, Corine H GeurtsvanKessel, Israt Jahan, Hubert P Endtz, Quazi D Mohammad, Bart C Jacobs
We describe the frequency, clinical features, and electrophysiological and immunological phenotypes of Guillain-Barré Syndrome (GBS) patients treated at a single institution in Bangladesh who had preceding chicken pox (primary Varicella-zoster virus [VZV] infection) within 4 weeks of GBS onset. A literature review of GBS cases preceding VZV infection is also provided. Diagnosis of GBS was based on the National Institute of Neurological Disorders and Stroke criteria for GBS. Serum anti-VZV IgM and IgG antibodies were quantified by indirect chemiluminescence immunoassay (CLIA); anti-Campylobacter jejuni IgG, IgM, and IgA antibodies and anti-ganglioside GM1 IgM and IgG antibodies, by enzyme-linked immunosorbent assays...
February 6, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29411188/antibiotic-treatment-and-stewardship-in-the-era-of-microbiota-oriented-diagnostics
#12
EDITORIAL
Debby Bogaert, Alex van Belkum
No abstract text is available yet for this article.
February 6, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29411187/effect-of-prior-dengue-infection-on-severity-and-outcome-of-japanese-encephalitis
#13
A Verma, A Jain, C Kumar, M Agarwal, R Kumar
Japanese encephalitis (JE) virus and dengue virus are closely related flaviviruses but interaction between them is scarcely studied in humans. The objective of this study was to compare the outcome of JE patients who are positive and negative for dengue IgG antibodies. Patients of acute encephalitis syndrome (AES) fulfilling predecided inclusion and exclusion criteria underwent a detailed standardized workup incorporating JE IgM testing in cerebrospinal fluid (CSF) or serum. Dengue IgG in serum was tested in all...
February 6, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29404837/correction-to-development-of-a-sensitive-pcr-dot-blot-assay-to-supplement-serological-tests-for-diagnosing-lyme-disease
#14
J S Shah, I D' Cruz, S Ward, N S Harris, R Ramasamy
In the original version of this article, the word "flagellin" is not correct. The correct word should be "P66" throughout the body of the article.
February 5, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29404836/characterization-of-the-nasopharyngeal-and-middle-ear-microbiota-in-gastroesophageal-reflux-prone-versus-gastroesophageal-reflux-non-prone-children
#15
Stefan A Boers, Marjolein de Zeeuw, Ruud Jansen, Marc P van der Schroeff, Annemarie M C van Rossum, John P Hays, Suzanne J C Verhaegh
Otitis media (OM) is one of the most common pediatric infections worldwide, but the complex microbiology associated with OM is poorly understood. Previous studies have shown an association between OM and gastroesophageal reflux (GER) in children. Therefore, in order to bridge the gap in our current understanding of the interaction between GER and OM, we investigated the nasopharyngeal and middle ear microbiota of children suffering from GER-associated OM and OM only, using culture-independent 16S rRNA gene sequencing...
February 5, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29397446/marseille-scoring-system-for-empiric-treatment-of-infective-endocarditis
#16
Frédérique Gouriet, Hervé Tissot-Dupont, Jean-Paul Casalta, Sandrine Hubert, Pierre-Edouard Fournier, Sophie Edouard, Alexis Theron, Hubert Lepidi, Dominique Grisoli, Gilbert Habib, Didier Raoult
Despite advances in medical, surgical, and critical care, infective endocarditis (IE) remains associated with considerable morbidity and mortality. We evaluated the performance of the Marseille score, including clinical data and biological tests obtained within 2 h, to identify patients at high risk of IE in order to initiate early antimicrobial treatment. This was secondarily confirmed using modified ESC criteria combined with molecular testing and (18)fluorodeoxyglucose-positron emission tomography/computed tomography as diagnostic tools...
February 3, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29396773/dolutegravir-in-pregnancy-effects-on-hiv-positive-women-and-their-infants
#17
Riikka Bornhede, Sandra Soeria-Atmadja, Katarina Westling, Karin Pettersson, Lars Navér
The development of new drugs for treatment of HIV has increased the efficacy and the quality of life together with decreased unwanted side-effect for people living with HIV. The integrase inhibitor dolutegravir has in short time become part of the first-line treatment in many countries, but is not a recommended first-line drug in pregnancy. As there are few publications of dolutegravir use during pregnancy, we found it valuable to analyze the Stockholm pregnancy cohort. A retrospective analysis of all pregnant women and their infants exposed to dolutegravir at Karolinska University Hospital, 2014-August 2017...
February 2, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29380226/infections-in-liver-and-lung-transplant-recipients-a-national-prospective-cohort
#18
Carlo Gagliotti, Filomena Morsillo, Maria Luisa Moro, Lucia Masiero, Francesco Procaccio, Francesca Vespasiano, Annalisa Pantosti, Monica Monaco, Giulia Errico, Andrea Ricci, Paolo Grossi, Alessandro Nanni Costa
Infections are a major complication of solid organ transplants (SOTs). This study aimed to describe recipients' characteristics, and the frequency and etiology of infections and transplant outcome in liver and lung SOTs, and to investigate exposures associated to infection and death in liver transplant recipients. The study population included recipients of SOTs performed in Italy during a 1-year period in ten Italian lung transplant units and eight liver transplant units. Data on comorbidities, infections, retransplantation, and death were prospectively collected using a web-based system, with a 6-month follow-up...
January 29, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29380225/considerable-variation-of-trough-%C3%AE-lactam-concentrations-in-older-adults-hospitalized-with-infection-a-prospective-observational-study
#19
Malini Hatti, Nikolitsa Solomonidi, Inga Odenholt, Johan Tham, Fredrik Resman
In older adults, few studies confirm that adequate concentrations of antibiotics are achieved using current dosage regimens of intravenous β-lactam antibiotics. Our objective was to investigate trough concentrations of cefotaxime, meropenem, and piperacillin in older adults hospitalized with infection. We included 102 patients above 70 years of age. Total trough antibiotic concentrations were measured and related to suggested target intervals. Information on antibiotic dose, patient characteristics, and 28-day outcomes were collected from medical records and regression models were fitted...
January 29, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29380224/prosthetic-valve-endocarditis-caused-by-propionibacterium-species-a-national-registry-based-study-of-51-swedish-cases
#20
Fredrik Lindell, Bo Söderquist, Kristina Sundman, Lars Olaison, Jan Källman
Propionibacterium spp. are a rare cause of infective endocarditis (IE). The diagnosis is difficult because the bacteria are slow-growing and growth in blood cultures is often misinterpreted as contamination from the skin flora. The aim of this study was to describe all cases of Propionibacterium spp. endocarditis in the Swedish national registry of IE. The registry was searched for all cases of IE from 1995 to 2016 caused by Propionibacterium spp. Data concerning clinical characteristics, treatment, and outcome were registered...
January 29, 2018: European Journal of Clinical Microbiology & Infectious Diseases
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