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European Journal of Clinical Microbiology & Infectious Diseases

Samuel Phillips, Lenka A Vodstrcil, Wilhelmina M Huston, Amba Lawerence, Peter Timms, Marcus Y Chen, Karen Worthington, Ruthy McIver, Catriona S Bradshaw, Suzanne M Garland, Sepehr N Tabrizi, Jane S Hocking
Spontaneous resolution of urogenital Chlamydia trachomatis (CT) without treatment has previously been described, but a limitation of these reports is that DNA or RNA-based amplification tests used do not differentiate between viable infection and non-viable DNA. We modified a previously published CT mRNA detection (omp2) method to differentiate between viable infection and non-viable DNA in a sample of CT DNA PCR positive women. We modified a CT mRNA detection (omp2) method from reverse transcriptase qPCR (RTqPCR) to digital PCR (dPCR) and evaluated it in samples from CT DNA positive women...
August 14, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Nuria Tormo, Eliseo Albert, Emilio Borrajo, Monserrat Bosque, Juan José Camarena, Victoria Domínguez, Encarnación Fuentes, Isabel Gascón, Bárbara Gomila, Nieves Gonzalo, Marta Jiménez, Olalla Martínez, José Miguel Nogueira, Nieves Orta, Josep Prat, Juan Carlos Rodríguez, Concepción Gimeno, David Navarro
A questionnaire-based cross-sectional study was conducted to gather information on current microbiological practices for active surveillance of carriage of multidrug-resistant (MDR) bacteria in hospitals from 14 health departments of the Autonomous Community of Valencia (ACV), Spain, which together provided medical attention to 3,271,077 inhabitants in 2017, approximately 70% of the population of the ACV. The survey consisted of 35 questions on MDR bacteria screening policies, surveillance approach chosen (universal vs...
August 13, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Chairut Vareechon, Javier Mestas, Claudia M Polanco, Jennifer Dien Bard
High accuracy of direct from positive blood culture molecular panels is imperative, particularly for the detection of resistance determinants as it allows for antimicrobial optimization prior to conventional susceptibility testing. In this study, we provide extensive data since implementation of the Verigene Gram-positive blood culture panel (BC-GP) in 2013. Within 5 years, 1636 blood culture bottles positive for a Gram-positive organism were tested on the BC-GP panel. The BC-GP panel identified 1520 Gram-positive organisms in 1636 (92...
August 11, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Maria J G T Vehreschild, Surabhi Taori, Simon D Goldenberg, Florian Thalhammer, Emilio Bouza, Joop van Oene, Graham Wetherill, Areti Georgopali
Information is limited or lacking on fidaxomicin treatment of Clostridium difficile infection (CDI) in patients with inflammatory bowel disease, fulminant or life-threatening CDI, severe renal impairment, moderate-to-severe hepatic impairment and pregnancy. The ANEMONE study investigated fidaxomicin use in a routine clinical setting, focusing on these medical conditions of specific interest (MCSIs). This retrospective, post-authorisation study reviewed hospital records from Austria, Germany, Spain and the UK (June 2012-June 2015), collecting data from hospital admission to 30 days after last fidaxomicin dose...
August 11, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Muhammad Nasir Ayub Khan, Daniëlle M L Verstegen, Abu Bakar Hafeez Bhatti, Diana H J M Dolmans, Walther Nicolaas Anton van Mook
The study aims to find the factors hindering the implementation of surgical site infection control guidelines in the operating rooms of low-income countries. The design of the study is a mixed-method sequential explanatory study. The setting is Shifa International Hospital and Pakistan Institute of Medical Sciences, Islamabad, Pakistan. Participants are health care workers. A questionnaire and structured key informant interviews probed the perspectives and perceptions of different stakeholders regarding the factors which hinder the implementation of surgical site infection control guidelines...
August 10, 2018: European Journal of Clinical Microbiology & Infectious Diseases
H Ciesielczuk, M Wilks, S Castelain, M Choquet, M Morotti, E Pluquet, V Sambri, M Tassinari, S Zannoli, L Cavalié, H Dupont, H Guet-Revillet
Intra-abdominal infections (IAIs) are one of the most common type of infections in patients with sepsis and an important cause of death in intensive care units. Early detection and treatment are necessary to reduce patient complications and improve outcomes. The Unyvero IAI Application (Curetis GmbH) is the first automated assay to rapidly and simultaneously identify a large panel of bacteria, fungi, toxins, and antibiotic resistance markers directly from IAI-related samples. The assay was evaluated in four European clinical laboratories in comparison to routine microbiological practices...
August 10, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Fatemah Sadeghpour Heravi, Vajihe Sadat Nikbin, Masomeh Nakhost Lotfi, Pouran Badiri, Nazanin Jannesar Ahmadi, Seyed Mohsen Zahraei, Fereshteh Shahcheraghi
Despite global efforts and widespread vaccination to control whooping cough (pertussis) caused by B. pertussis, the re-emergence of pertussis still is being reported all over the world. Antigenic divergence in B. pertussis virulence factors is one of the reasons of pertussis resurgence, resulting in dissimilarity of local and vaccine strains. In this study, clonal spread and variation of B. pertussis virulence factor in isolated strains from Iranian patients have been analyzed. A total of 100 B. pertussis isolates were obtained from Pertussis Reference Laboratory of Pasteur Institute of Iran...
August 9, 2018: European Journal of Clinical Microbiology & Infectious Diseases
O Lesens, T Ferry, E Forestier, E Botelho-Nevers, P Pavese, E Piet, B Pereira, E Montbarbon, B Boyer, S Lustig, S Descamps
To evaluate factors associated with failure in patients treated with DAIR (debridement, antibiotic therapy, and implant retention) for Staphylococcus aureus prosthetic joint infections (PJIs). We retrospectively analyzed consecutive patients with stable PJI due to S. aureus treated with DAIR at six hospitals between 2010 and 2014. Cox proportional hazards regression was used to study factors associated with treatment failure at 2 years. Of 154 eligible patients, 137 were included (mean age 73 ± 13 years; male 56%)...
August 7, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Rasmus K B Richelsen, Signe B Jensen, Henrik Nielsen
To assess the incidence, predictive factors, and prognosis of acyclovir-induced nephrotoxicity. We conducted a historical prospective cohort study of patients treated with intravenous acyclovir in North Denmark Region from 2009 to 2016. Information on baseline demographics, co-morbidities, plasma creatinine, and treatment was obtained from the medical records. The primary outcome was an increase of ≥ 40 μmol/L in plasma creatinine level from baseline. We included 276 patients treated with intravenous acyclovir of which 29 (10...
August 7, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Anna J Henningsson, Malin Lager, Rebecka Brännström, Ivar Tjernberg, Barbro H Skogman
Anti-Borrelia antibodies in the cerebrospinal fluid (CSF) are required for definite diagnosis of Lyme neuroborreliosis (LNB). However, children often present with early LNB, and antibody production in the CSF may not be demonstrated. Recent studies have suggested the chemokine CXCL13 to be an early marker for LNB. The aim of the study was to evaluate CXCL13 for laboratory diagnosis in pediatric LNB patients and to evaluate the association with pleocytosis in CSF, clinical features, and recovery. CSF samples were collected from LNB patients, classified as definite LNB (n = 44) or possible LNB (n = 22), and controls classified as non-LNB (n = 102) or other specific diagnoses (n = 23)...
August 6, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Evangelos J Giamarellos-Bourboulis, Antigone Kotsaki, Christina Routsi, Elena Graziano, Elda Righi, Matteo Bassetti
The management of bacteremia by carbapenem-resistant Gram-negative bacteria (CRGNB) necessitates a surrogate marker for response to treatment. We developed a prognostic score of bacteremia resolution using a test and a validation cohort. In the test cohort, five protein biomarkers were measured in serial daily serum samples from 39 patients with ventilator-associated pneumonia (VAP) and CRGNB bacteremia. Receiver operator characteristic curves were designed to identify cut-off of over-time changes that were associated with more than 80% specificity for resolution of bacteremia...
August 6, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Maricela Valerio, Antonio Vena, Carmen Guadalupe Rodríguez-González, Esther Chamorro de Vega, Miriam Mateos, Maria Sanjurjo, Emilio Bouza, Patricia Muñoz
A previous audit to assess the quality of antifungal use was performed in our hospital in 2011. After 5 years of antifungal stewardship program (AFS), we performed a follow-up audit in order to describe the long-term effect of such program. Using a predefined score, we evaluated the antifungal use in 100 consecutive adult inpatients receiving systemic antifungals. Results of the present audit were compared with those of a previous one, performed in 2011, before the implementation of our AFS. After 5 years, AFS program has induced a change in the population who received antifungal drugs in our hospital with a reduction in medical patients and a relative higher prescription among hematological ones...
August 4, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Thais Larrainzar-Coghen, Dolors Rodríguez-Pardo, Pere Barba, Juan Aguilar-Company, Virginia Rodríguez, Gloria Roig, Carmen Ferrer, Isabel Ruiz-Camps, Benito Almirante
The aim of the study is to evaluate demographics, epidemiology, clinical characteristics, treatment and outcomes of Clostridium difficile infection (CDI) in patients with and without concurrent cancer. This is a prospective cohort study of consecutive primary CDI episodes in adults (January 2006-December 2016). CDI was diagnosed on the presence of diarrhoea and positive stool testing for toxigenic C. difficile. Univariate analysis assessed differences between cancer and non-cancer patients. Risk factors of all-cause 30-day mortality were determinate using the logistic multivariable procedure...
August 2, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Stéphane Gennai, Stéphanie Ortiz, Bastien Boussat, Patrice François, Patricia Pavese
To evaluate the relevance of ceftriaxone prescriptions in an emergency department of a university hospital and suggest whenever possible an antibiotic alternative with a lower ecological impact. All ceftriaxone prescriptions in the first complete week of each month during the year 2016, in the emergency department of the Grenoble university hospital, have been analyzed by an IDS referent in antibiotic prescriptions. Ceftriaxone prescription was considered appropriate if justified (an antibiotic must be used), relevant (ceftriaxone is a good choice), and adapted (in terms of dose, route, and period of administration), regardless of a potential antibiotic association, consistent with international recommendations...
August 1, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Mark R Hobbs, Cameron C Grant, Mark G Thomas, Sarah Berry, Susan M B Morton, Emma Marks, Stephen R Ritchie
New Zealand children suffer from high rates of skin and soft tissue infection (SSTI). Staphylococcus aureus colonisation is known to increase the risk of nosocomial infection. We aimed to determine whether S. aureus colonisation also increased the risk of community-onset SSTI. This study, performed within the Growing Up in New Zealand cohort, used interview and administrative data, and bacterial culture results from the nose, throat, and skin swabs collected at 4½ years of age. Multivariable log-binomial regression was used to derive adjusted risk ratios...
July 31, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Giulia la Martire, Christine Robin, Nadia Oubaya, Raphaël Lepeule, Florence Beckerich, Mathieu Leclerc, Walid Barhoumi, Andréa Toma, Cécile Pautas, Sébastien Maury, Wiem Akrout, Catherine Cordonnier-Jourdin, Vincent Fihman, Mario Venditti, Catherine Cordonnier
Febrile neutropenia (FN) is the main reason for antibiotic prescription in hematology wards where, on the other hand, antibiotic stewardship (AS) is poorly explored. The objectives of the present study were to evaluate (1) the impact of an AS intervention on antibiotic consumption and (2) the applicability and acceptance rate of the intervention and its clinical impact. A persuasive AS intervention based on European Conference on Infection in Leukaemia (ECIL) guidelines for FN was implemented in a high-risk hematology ward in a tertiary referral public university hospital...
July 26, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Ting Yang, Yanping Zou, Weili Zhou, Zhi Ruan, Yingying Kong, Yunheng Zhou, Jun Zhang, Xinyou Xie
Whether Ureaplasma spp. are a causative agent of male infertility remains controversial. Previous studies concerning Ureaplasma spp. and male infertility have been confined to the species level of Ureaplasma. Currently, an expanded multilocus sequence typing (eMLST) scheme has been established with high discriminatory power. The aim of this study was to use eMLST to explore the distribution of Ureaplasma spp. and to analyze its role in oligozoospermia and semen quality. A total of 480 semen samples were obtained from Chinese infertile males...
July 26, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Herlice do Nascimento Veras, Pedro H Q S Medeiros, Samilly A Ribeiro, Thiago M Freitas, Ana K S Santos, Marília S M G Amaral, Mariana D Bona, Alexandre Havt, Ila F N Lima, Noélia L Lima, Alessandra Di Moura, Álvaro M Leite, Alberto M Soares, José Q Filho, Richard L Guerrant, Aldo A M Lima
Campylobacter spp. have been associated with anthropometric Z-score decrements, but the role of specific virulence genes associated with these outcomes has not been explored. This study aimed to investigate whether specific Campylobacter jejuni virulence-related gene and immune-inflammatory biomarkers are associated with malnutrition in children from Northeastern Brazil. A case-control study was performed in Fortaleza, Brazil. Children aging 6-24 months were characterized as malnourished (cases) if weight-for-age Z-score (WAZ) = 2 and as nourished (controls) if WAZ ≥ 1...
July 26, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Siyuan Yao, Shintaro Yagi, Miki Nagao, Ryuji Uozumi, Taku Iida, Sena Iwamura, Yosuke Miyachi, Hisaya Shirai, Atsushi Kobayashi, Shinya Okumura, Yuhei Hamaguchi, Yuuki Masano, Toshimi Kaido, Hideaki Okajima, Shinji Uemoto
The interpretation of bacterial cholangitis after liver transplantation (LT) remains vague, because the presence of bacteria in bile, namely bacteriobilia, does not necessarily indicate an active infection. We investigated the association between post-LT bacterial cholangitis and a variety of short- and long-term outcomes. Two-hundred-seventy-four primary adult-to-adult living donor LT recipients from 2008 to 2016 were divided into three groups according the presence or absence of bacteriobilia and clinical symptoms: (1) no bacteriobilia (N group), (2) asymptomatic bacteriobilia (B group), and (3) cholangitis (C group)...
July 23, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Sophie Edouard, Matthieu Million, Dipankar Bachar, Grégory Dubourg, Caroline Michelle, Laetitia Ninove, Rémi Charrel, Didier Raoult
The nasopharynx is the primary site of colonization by respiratory pathogen that constitutes the port of entrance in the respiratory tract. The role of mucosal respiratory microbiota in infection has been recently emphasized; therefore, we aimed to assess if a specific respiratory microbiota profile was associated with symptomatic infection and/or with presence of respiratory viruses. We performed a case-control study to characterize the healthy respiratory microbiota and its alteration during acute viral infections...
July 22, 2018: European Journal of Clinical Microbiology & Infectious Diseases
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