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Virus Genes

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https://www.readbyqxmd.com/read/27933433/full-length-infectious-clone-of-an-iranian-isolate-of-chicken-anemia-virus
#1
Amir Kaffashi, Fatemeh Eshratabadi, Abdelhamed Shoushtari
An Iranian field strain of chicken anemia virus (CAV), designated IR CAV, was isolated in the Marek's disease virus-transformed lymphoblastoid cell line MDCC-MSB1 (MSB1) culture for the first time. The full-length CAV DNA of this strain was cloned in the bacterial plasmid pTZ57R/T to create the molecular clone pTZ-CAV. The nucleotide and deduced amino acid sequences of viral proteins of IR CAV were compared with those of representative CAV sequences including reference and commercial vaccine strains. IR CAV was not related to vaccine strains and also found to have glutamine at positions 139 and 144 confirming previous studies in which such mutations were associated with a slow rate of virus spread in cell culture...
December 8, 2016: Virus Genes
https://www.readbyqxmd.com/read/27913980/complete-nucleotide-sequences-and-construction-of-full-length-infectious-cdna-clones-of-cucumber-green-mottle-mosaic-virus-cgmmv-in-a-versatile-newly-developed-binary-vector-including-both-35s-and-t7-promoters
#2
Chan-Hwan Park, Hye-Kyoung Ju, Jae-Yeong Han, Jong-Seo Park, Ik-Hyun Kim, Eun-Young Seo, Jung-Kyu Kim, John Hammond, Hyoun-Sub Lim
Seed-transmitted viruses have caused significant damage to watermelon crops in Korea in recent years, with cucumber green mottle mosaic virus (CGMMV) infection widespread as a result of infected seed lots. To determine the likely origin of CGMMV infection, we collected CGMMV isolates from watermelon and melon fields and generated full-length infectious cDNA clones. The full-length cDNAs were cloned into newly constructed binary vector pJY, which includes both the 35S and T7 promoters for versatile usage (agroinfiltration and in vitro RNA transcription) and a modified hepatitis delta virus ribozyme sequence to precisely cleave RNA transcripts at the 3' end of the tobamovirus genome...
December 2, 2016: Virus Genes
https://www.readbyqxmd.com/read/27913979/genetic-diversity-of-the-genotype-vii-newcastle-disease-virus-identification-of-a-novel-viij-sub-genotype
#3
Cong Xue, Yanlong Cong, Renfu Yin, Yixue Sun, Chan Ding, Shengqing Yu, Xiufan Liu, Shunlin Hu, Jing Qian, Qianliang Yuan, Mingxi Yang, Chunfeng Wang, Zhuang Ding
Newcastle disease (ND) is a highly contagious disease of poultry caused by Newcastle disease virus (NDV). Multiple genotypes of NDV have been circulating worldwide and NDV is continuously evolving, resulting into more diversity. Of multiple viral genotypes, VII is particularly important given that it had been associated with most recent ND outbreaks worldwide. In this study, an epidemiological investigation performed in northeastern China during 2014-2015 showed that 11 genotype VII isolates amounted to 55 percent in a total number of NDV isolates...
December 2, 2016: Virus Genes
https://www.readbyqxmd.com/read/27900587/phylogenetic-analysis-of-wheat-dwarf-virus-isolates-from-iran
#4
Mohamad Hamed Ghodoum Parizipour, Jörg Schubert, Seyed Ali Akbar Behjatnia, Alireza Afsharifar, Antje Habekuß, Beilei Wu
Wheat dwarf virus (WDV) adversely affects cereal production in Asia, Europe, and North Africa. In this study, sequences of several WDV isolates from Iran which is located in the Fertile Crescent were analyzed. Analysis revealed a new geographic cluster for WDV-Wheat from Iran. Recombination analysis demonstrated the existence of several breakpoints in different regions of the viral genome. Data analysis demonstrated that WDV-Barley has an older history and lower diversity than WDV-Wheat. Sequence analysis identified a rare occasion of a co-infection of wheat with WDV-Wheat and WDV-Barley...
November 30, 2016: Virus Genes
https://www.readbyqxmd.com/read/27891562/molecular-genetic-analysis-and-evolution-of-begomoviruses-and-betasatellites-causing-yellow-mosaic-disease-of-bhendi
#5
R Vinoth Kumar, H C Prasanna, A K Singh, D Ragunathan, G K Garg, S Chakraborty
In India, Bhendi yellow vein mosaic disease (BYVMD) is one of the most economically important diseases of bhendi/okra and is caused by a complex of monopartite begomovirus (Bhendi yellow vein mosaic virus-BYVMV) and betasatellite (Bhendi yellow vein betasatellite-BYVB). In this study, we have analyzed the role of possible evolutionary factors involved in the evolution of BYVMV and BYVB isolates. Evidence of inter-species and inter-strain recombination events was detected among the viral isolates, and majority of these recombinant isolates possess microsatellites in their genome...
November 28, 2016: Virus Genes
https://www.readbyqxmd.com/read/27866318/pig-bvdv-2-non-structural-protein-n-pro-links-to-cellular-antiviral-response-in-vitro
#6
Jie Tao, Jinhu Liao, Jianye Wang, Xinjun Zhang, Qian Zhang, Liqian Zhu, Weiping Zhang, Huili Liu, Guoqiang Zhu
In this study, we constructed for the first time a full-length cDNA clone of pig-original bovine viral diarrhea virus 2 (BVDV-2) strain SH-28, modified the cDNA clone (pASH28) for mutant pASHΔN(pro) and derived virus strain vASHΔN(pro) by deleting the genomic region encoding the N(pro) polypeptide, and examined significance of protein N(pro) for antiviral responses in vitro. Data showed that N(pro)-deletion mutant virus vASHΔN(pro) led to significant overexpression of oligo adenylate synthetase (OAS), myxovirus-resistant protein 1 (Mx1), and ubiquitin-like protein 15 (ISG15)...
November 19, 2016: Virus Genes
https://www.readbyqxmd.com/read/27858313/complete-genomic-sequence-of-an-infectious-pancreatic-necrosis-virus-isolated-from-rainbow-trout-oncorhynchus-mykiss-in-china
#7
Feng Ji, Jing-Zhuang Zhao, Miao Liu, Tong-Yan Lu, Hong-Bai Liu, Jiasheng Yin, Li-Ming Xu
Infectious pancreatic necrosis (IPN) is a significant disease of farmed salmonids resulting in direct economic losses due to high mortality in China. However, no gene sequence of any Chinese infectious pancreatic necrosis virus (IPNV) isolates was available. In the study, moribund rainbow trout fry samples were collected during an outbreak of IPN in Yunnan province of southwest China in 2013. An IPNV was isolated and tentatively named ChRtm213. We determined the full genome sequence of the IPNV ChRtm213 and compared it with previously identified IPNV sequences worldwide...
November 18, 2016: Virus Genes
https://www.readbyqxmd.com/read/27858312/isolation-and-complete-genome-characterization-of-novel-reassortant-orthoreovirus-from-common-vole-microtus-arvalis
#8
Enikő Fehér, Gábor Kemenesi, Miklós Oldal, Kornélia Kurucz, Renáta Kugler, Szilvia L Farkas, Szilvia Marton, Győző Horváth, Krisztián Bányai, Ferenc Jakab
A novel mammalian orthoreovirus (MRV) strain was isolated from the lung tissue of a common vole (Microtus arvalis) with Tula hantavirus infection. Seven segments (L1-L3, M2-M3, S2, and S4) of the Hungarian MRV isolate MORV/47Ma/06 revealed a high similarity with an MRV strain detected in bank vole (Myodes glareolus) in Germany. The M1 and S3 segment of the Hungarian isolate showed the closest relationship with the sequence of a Slovenian human and a French murine isolate, respectively. The highest nucleotide and amino acid identity values were above 90 and 95% in all of the comparisons to the reference sequences in GenBank, except for the S1 with a maximum of 69...
November 17, 2016: Virus Genes
https://www.readbyqxmd.com/read/27848068/inhibition-of-porcine-transmissible-gastroenteritis-virus-infection-in-porcine-kidney-cells-using-short-hairpin-rnas-targeting-the-membrane-gene
#9
Li Wang, Xianjin Dai, Han Song, Peng Yuan, Zhou Yang, Wei Dong, Zhenhui Song
The membrane (M) protein is the most abundant component of the porcine transmissible gastroenteritis virus (TGEV) particle. To exploit the possibility of using RNA interference (RNAi) as a strategy against TGEV infection, three plasmids (pRNAT-1, pRNAT-2, and pRNAT-3) expressing short hairpin RNAs were designed to target three different coding regions of the M gene of TGEV. The plasmids were constructed and transiently transfected into a porcine kidney cells, PK-15, to determine whether these constructs inhibited TGEV production...
November 15, 2016: Virus Genes
https://www.readbyqxmd.com/read/27838879/genetic-analysis-of-h9n2-avian-influenza-viruses-circulated-in-broiler-flocks-a-case-study-in-iraq-in-2014-2015
#10
Qayssar Ali Kraidi, Omid Madadgar, Arash Ghalyanchi Langeroudi, Vahid Karimi
H9N2 avian influenza viruses (AIVs) have been recorded in Eurasian for several years. Since 2004-2005, the disease has become endemic in Iraq, causing serious economic losses in the poultry industry. The hemagglutinin (HA) and neuraminidase (NA), two out of eight protein-coding genes, play an important role during the early stage of infection and hinder virus assembling. Little is known about the genetic information of the H9N2 viruses currently circulating in Iraq; thus, gene sequences of six AIVS of the H9N2 subtype have been detected and analyzed in the period of 2014-2015 from different outbreaks of broiler flocks in five provinces situated in the middle and southern parts of Iraq...
November 12, 2016: Virus Genes
https://www.readbyqxmd.com/read/27817150/genome-characterization-of-a-bovine-papillomavirus-type-5-from-cattle-in-the-amazon-region-brazil
#11
Flavio R C da Silva, Cíntia Daudt, Samuel P Cibulski, Matheus N Weber, Ana Paula M Varela, Fabiana Q Mayer, Paulo M Roehe, Cláudio W Canal
Papillomaviruses are small and complex viruses with circular DNA genome that belongs to the Papillomavirus family, which comprises at least 39 genera. The bovine papillomavirus (BPV) causes an infectious disease that is characterized by chronic and proliferative benign tumors that affect cattle worldwide. In the present work, the full genome sequence of BPV type 5, an Epsilonpapillomavirus, is reported. The genome was recovered from papillomatous lesions excised from cattle raised in the Amazon region, Northern Brazil...
November 5, 2016: Virus Genes
https://www.readbyqxmd.com/read/27815750/a-sensitive-duplex-nanoparticle-assisted-pcr-assay-for-identifying-porcine-epidemic-diarrhea-virus-and-porcine-transmissible-gastroenteritis-virus-from-clinical-specimens
#12
Yu Zhu, Lin Liang, Yakun Luo, Guihua Wang, Chunren Wang, Yudong Cui, Xia Ai, Shangjin Cui
In this study, a novel duplex nanoparticle-assisted polymerase chain reaction (nanoPCR) assay was developed to detect porcine epidemic diarrhea virus (PEDV) and porcine transmissible gastroenteritis virus (TGEV). Two pairs of primers were designed based on the conserved region within the N gene of PEDV and TGEV. In a screening of 114 clinical samples from four provinces in China for PEDV and TGEV, 48.2 and 3.5 % of the samples, respectively, tested positive. Under optimized conditions, the duplex nanoPCR assay had a detection limit of 7...
November 4, 2016: Virus Genes
https://www.readbyqxmd.com/read/27796639/complete-genome-sequence-of-the-lytic-cold-active-pseudomonas-fluorescens-bacteriophage-vsw-3-from-napahai-plateau-wetland
#13
Chunjing Zhang, Zhongyao Zhang, Jiankai Li, Kunhao Qin, Yunlin Wei, Qi Zhang, Lianbing Lin, Xiuling Ji
The lytic cold-active bacteriophage VSW-3, belonging to the Podoviridae family and infecting the host Pseudomonas fluorescens SW-3, was isolated from the Napahai plateau wetland in China. With the development of sequencing technology, the study of Pseudomonas genomic diversity has increased; however, knowledge of cold-active phages infecting Pseudomonas is limited. The newly sequenced phage VSW-3 was classified based on virion morphology by transmission electron microscope. Sequence analysis revealed that the genome size was 40,556 bp with an overall GC content of 57...
October 31, 2016: Virus Genes
https://www.readbyqxmd.com/read/27738905/tanapoxvirus-lacking-a-neuregulin-like-gene-regresses-human-melanoma-tumors-in-nude-mice
#14
Tiantian Zhang, Yogesh R Suryawanshi, Dennis H Kordish, Helene M Woyczesczyk, David Jeng, Karim Essani
Neuregulin (NRG), an epidermal growth factor is known to promote the growth of various cell types, including human melanoma cells through ErbB family of tyrosine kinases receptors. Tanapoxvirus (TPV)-encoded protein TPV-15L, a functional mimic of NRG, also acts through ErbB receptors. Here, we show that the TPV-15L protein promotes melanoma proliferation. TPV recombinant generated by deleting the 15L gene (TPVΔ15L) showed replication ability similar to that of wild-type TPV (wtTPV) in owl monkey kidney cells, human lung fibroblast (WI-38) cells, and human melanoma (SK-MEL-3) cells...
October 13, 2016: Virus Genes
https://www.readbyqxmd.com/read/27738904/characterization-of-clade-2-3-4-4-h5n8-highly-pathogenic-avian-influenza-viruses-from-wild-birds-possessing-atypical-hemagglutinin-polybasic-cleavage-sites
#15
Tatsufumi Usui, Kosuke Soda, Yukiko Tomioka, Hiroshi Ito, Toshiyo Yabuta, Hiroki Takakuwa, Koichi Otsuki, Toshihiro Ito, Tsuyoshi Yamaguchi
Since 2014, clade 2.3.4.4 H5 subtype highly pathogenic avian influenza viruses (HPAIVs) have been distributed worldwide. These viruses, which were reported to be highly virulent in chickens by intravenous inoculation, have a consensus HPAI motif PLRERRRKR at the HA cleavage site. However, two-clade 2.3.4.4 H5N8 viruses which we isolated from wild migratory birds in late 2014 in Japan possessed atypical HA cleavage sequences. A swan isolate, Tottori/C6, had a novel polybasic cleavage sequence, PLGERRRKR, and another isolate from a dead mandarin duck, Gifu/01, had a heterogeneous mixture of consensus PLRERRRKR and variant PLRERRRRKR sequences...
October 13, 2016: Virus Genes
https://www.readbyqxmd.com/read/27734222/characterization-of-batai-virus-isolated-from-a-domestic-muscovy-duck-cairina-moschate
#16
Lijiao Zhang, Qingshui Zhang, Jun Wang, Na An, Yanxin Cao, Guanghua Fu, Xueying Hu, Yu Huang, Jingliang Su
Batai virus (BATV) belongs to the genus Orthobunyavirus of the family Bunyaviridae. It has been isolated from mosquitos, pigs, cattle, and humans throughout Africa, Asia, and Europe, and causes clinical signs in domestic animals and humans. Here, we report the isolation of BATV from a domestic duck flock. Genome sequence analysis revealed clustering of this isolate in the Africa-Asia lineage. The virus replicated in mosquitos and vertebrate host cells, showing different phenotypic characteristics, and showed the potential to infect mice...
October 12, 2016: Virus Genes
https://www.readbyqxmd.com/read/27730428/construction-of-a-full-length-infectious-cdna-clone-of-cowpea-mild-mottle-virus
#17
Silvia L Carvalho, Tatsuya Nagata, Bruna R Junqueira, Larissa G Zanardo, Ana C S Paiva, Claudine M Carvalho
Infectious cDNA clones are an important tool to study the molecular and cellular process of RNA virus infection. In vitro and in vivo transcription systems are the two main strategies used in the generation of infectious cDNA clones for RNA viruses. This study describes the first generation of a full-length infectious cDNA clone of Cowpea mild mottle virus (CPMMV), a Carlavirus. The full-length genome was synthesized by Overlap Extension PCR of two overlapping fragments and cloned in a pUC-based vector under control of the SP6 RNA polymerase promoter...
October 11, 2016: Virus Genes
https://www.readbyqxmd.com/read/27730427/characterization-of-a-reassortant-h11n9-subtype-avian-influenza-virus-isolated-from-bean-goose-along-the-east-asian-australian-flyway
#18
Yanfeng Yao, Zhiyong Shao, Bin He, Wenhai Yang, Jianjun Chen, Tao Zhang, Xiabing Chen, Jie Chen
During the surveillance of avian influenza viruses in the Dongxi Lake wetland of Hubei in 2015-2016, an H11N9 avian influenza virus was isolated from a bean goose (Anser fabalis). Phylogenetic analysis showed that the HA gene of this isolate belongs to the North American lineage; however, the NA and the internal genes of the isolate were generated from the Eurasian lineage. This strain had reduced pathogenicity in mice and was capable of replication in the mouse lung without prior adaptation. This is the first report detecting H11N9 subtype influenza virus from migratory birds in central China...
October 11, 2016: Virus Genes
https://www.readbyqxmd.com/read/27718047/generation-and-evaluation-of-a-genetically-attenuated-newcastle-disease-virus-rgm-viim-as-a-genotype-matched-vaccine
#19
Minhua Sun, Bin Xiang, Yaling Li, Peng Xie, Shimin Gao, Yinfeng Kang, Pei Gao, Yanling Li, Zhaoxiong Wang, Jianpeng Liang, Deshui Yu, Tao Ren
Despite intensive vaccination campaigns, outbreaks of Newcastle disease (ND) have been frequently reported in China, especially of genotype VII that first emerged in the late 1990s. Given the dire need for vaccines against the circulating genotype VII virus, we developed an alternative method to recover a highly virulent recombinant GM (rGM) virus that involves a T7 system with a hammerhead ribozyme sequence introduced downstream of the T7 promoter. By changing the F0 polybasic cleavage site RRQKR↓F to the monobasic GRQGR↓L, we generated a mutant virus (rGM-VIIm) that was found to be highly attenuated in chickens...
October 7, 2016: Virus Genes
https://www.readbyqxmd.com/read/27714496/community-and-hospital-acquired-infections-with-oseltamivir-and-peramivir-resistant-influenza-a-h1n1-pdm09-viruses-during-the-2015-2016-season-in-japan
#20
Akinobu Hibino, Hiroki Kondo, Hironori Masaki, Yoshinari Tanabe, Isamu Sato, Nobuhiro Takemae, Takehiko Saito, Hassan Zaraket, Reiko Saito
We report five cases of community- and hospital-acquired infections with oseltamivir- and peramivir-resistant A(H1N1)pdm09 viruses possessing the neuraminidase (NA) H275Y mutation during January-February 2016 in Japan. One case was hospitalized and was receiving oseltamivir for prophylaxis. The remaining four cases were not taking antiviral drugs at the time of sampling. These cases were geographically distant and epidemiologically unrelated. The five viruses showed ~300-fold rise in IC50 values against oseltamivir and peramivir, defined as highly reduced inhibition according to the WHO definition...
October 6, 2016: Virus Genes
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