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APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica

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https://www.readbyqxmd.com/read/28332248/citrate-attenuates-vascular-calcification-in-chronic-renal-failure-rats
#1
Yan Ou, Zengying Liu, Shuiqin Li, Xiaojing Zhu, Yan Lin, Jin Han, Zhaoyang Duan, Lining Jia, Baosong Gui
Vascular calcification (VC) is a major contributor of cardiovascular dysfunction in chronic renal failure (CRF). Citrate binds calcium and inhibits the growth of calcium crystals. This present study intends to evaluate the effect of citrate on VC in adenine-induced CRF rats. The rats were randomly divided into five groups: the control group, the citrate control group, model group, model rats with low-dose treatment of citrate (216 mg/kg) and model rats with high-dose treatment of citrate (746 mg/kg). The rats were euthanized at 5 weeks with their blood and aorta in detection...
March 23, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28332241/toll-like-receptor-2-arg753gln-and-arg677trp-polymorphisms-and-susceptibility-to-pulmonary-and-peritoneal-tuberculosis
#2
Mohammed A Saleh, Mahmoud M Ramadan, Eman O Arram
Recent evidence suggests that toll-like receptor-2 (TLR2) is important for host defense against Mycobacterium tuberculosis (MTB). TLR2 polymorphisms have shown significant impact on susceptibility or resistance to tuberculosis (TB). This case-control study aims to determine the influence of TLR2 (Arg753Gln and Arg677Trp) polymorphisms on the susceptibility to develop pulmonary or peritoneal TB. Genotyping of TLR2 (Arg753Gln and Arg677Trp) polymorphisms was carried out on 52 patients with pulmonary TB, 44 patients with peritoneal TB, and 50 healthy controls using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)...
March 23, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28295672/the-prevalence-of-hpv-infections-in-hpv-vaccinated-women-from-the-general-population
#3
Eva Hamsikova, Jana Smahelova, Viera Ludvikova, Martina Salakova, Jana Rychla, Jana Skrenkova, Lukas Rob, Ruth Tachezy
Currently, three prophylactic HPV vaccines are commercially available to prevent HPV 16/18 infection and associated lesions. The aim of the study was to assess markers of HPV infection in women/girls before vaccination and to ascertain the prevalence and spectrum of post-vaccination HPV types. Three hundred and thirty subjects of which 75 were virgins were enrolled. Before the first dose of the HPV vaccine and 1, 3 and 5 years after the completion of HPV vaccination, the samples for cytology, HPV detection and anti-HPV antibody response were taken...
March 15, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28295668/dissemination-of-metallo-%C3%AE-lactamase-in-pseudomonas-aeruginosa-isolates-in-egypt-mutation-in-blavim-4
#4
Hany Hashem, Amro Hanora, Salah Abdalla, Alaa Shaeky, Alaa Saad
This study was designed to investigate the prevalence of metallo-β-lactamase (MBL) in Pseudomonas aeruginosa isolates collected from Suez Canal University Hospital in Ismailia, Egypt. Antibiotic susceptibility testing and phenotypic and genotypic screening for MBLs were performed on 147 isolates of P. aeruginosa. MICs were determined by agar dilution method for carbapenem that was ≥2 μg/mL for meropenem. MBL genes were detected by multiplex and monoplex PCR for P. aeruginosa-harbored plasmids. Mutation profile of sequenced MBL genes was screened using online software Clustal Omega...
March 15, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28295617/carbapenemases-producing-klebsiella-pneumoniae-in-hospitals-of-two-regions-of-southern-italy
#5
Carla Calia, Carlo Pazzani, Marta Oliva, Maria Scrascia, Piero Lovreglio, Carmen Capolongo, Anna Maria Dionisi, Adriana Chiarelli, Rosa Monno
Carbapenem-resistant Klebsiella pneumoniae infections are reported with increasing frequency elsewhere in the world, representing a worrying phenomenon for global health. In Italy, there are hotspot data on the diffusion and type of carbapenemase-producing Enterobacteriaceae and K. pneumoniae in particular, with very few data coming from Apulia and Basilicata, two regions of Southern Italy. This study was aimed at characterizing by phenotypic and genotypic methods carbapenem-resistant K. pneumoniae isolated from several Hospitals of Apulia and Basilicata, Southern Italy...
March 15, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28294414/evaluation-of-phytochemicals-from-medicinal-plants-of-myrtaceae-family-on-virulence-factor-production-by-pseudomonas-aeruginosa
#6
Khadar Syed Musthafa, Wipawadee Sianglum, Jongkon Saising, Sakkarin Lethongkam, Supayang Piyawan Voravuthikunchai
Virulence factors regulated by quorum sensing (QS) play a critical role in the pathogenesis of an opportunistic human pathogen, Pseudomonas aeruginosa in causing infections to the host. Hence, in the present work, the anti-virulence potential of the medicinal plant extracts and their derived phytochemicals from Myrtaceae family was evaluated against P. aeruginosa. In the preliminary screening of the tested medicinal plant extracts, Syzygium jambos and Syzygium antisepticum demonstrated a maximum inhibition in QS-dependent violacein pigment production by Chromobacterium violaceum DMST 21761...
March 15, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28294408/tumor-necrosis-factor-%C3%AE-308g-a-and-238g-a-polymorphisms-are-associated-with-increased-risks-of-sepsis-evidence-from-an-updated-meta-analysis
#7
Mu Zhang, Yu Zhao, Qiong Liu
Previous studies have reported the relationship between tumor necrosis factor-α (TNF-α) -308G/A and -238G/A polymorphisms and sepsis risk with inconsistent results. The aim of this study was to estimate the association of the two polymorphisms with risk of sepsis or sepsis-related mortality using a meta-analysis. PubMed, Embase, and Web of Science databases were searched up to June 20 2016. Pooled odds ratios (ORs) and 95% confidence intervals (CIs) were calculated using fixed or random effect model. Twenty-six studies were included in this meta-analysis...
March 15, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28256016/circulating-syndecans-during-critical-illness
#8
Axel Nelson, Joakim Johansson, Jonas Tydén, Mikael Bodelsson
Circulating syndecans are proposed to be markers of glycocalyx degradation and previous investigations have found higher plasma levels of syndecan-1 among patients with different pathological conditions. We wanted to investigate if levels of other syndecans (-2,-3 and -4) are altered during critical illness and compare the levels to syndecan-1. In 137 consecutive intensive care unit (ICU) patients with sepsis, cardiac arrest, gastrointestinal bleeding, intoxication or trauma, plasma levels of syndecan-1, -2, -3 and -4 were measured using ELISA...
March 3, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28225209/association-of-the-il-10-receptor-a536g-s138g-loss-of-function-variant-with-multiple-sclerosis-in-tunisian-patients
#9
Nadia Ben Fredj, Mouna Aissi, Walid Ben Selma, Imen Mahmoud, Faten Nefzi, Mahbouba Frih-Ayed, Jalel Boukadida, Mahjoub Aouni
Interleukin-10 (IL-10), a potent anti-inflammatory T-cell cytokine, has been shown to be a regulatory cytokine that is associated with disease remission in multiple sclerosis (MS) and exerts its activity through its cognate cell surface receptor complex, IL-10 receptor 1 (IL-10R1) and IL-10R2. The purpose of this study was to investigate the IL-10R1 S138G loss-of-function polymorphism (A536G: rs3135932) for possible influence on susceptibility and outcome of MS in Tunisian patients. A total of 103 Tunisian MS patients and 160 control subjects were studied...
February 22, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28225151/cytopathologic-differential-diagnosis-of-low-grade-urothelial-carcinoma-and-reactive-urothelial-proliferation-in-bladder-washings-a-logistic-regression-analysis
#10
Ebru Cakir, Ulku Kucuk, Emel Ebru Pala, Ozlem Sezer, Rahmi Gokhan Ekin, Ozgur Cakmak
Conventional cytomorphologic assessment is the first step to establish an accurate diagnosis in urinary cytology. In cytologic preparations, the separation of low-grade urothelial carcinoma (LGUC) from reactive urothelial proliferation (RUP) can be exceedingly difficult. The bladder washing cytologies of 32 LGUC and 29 RUP were reviewed. The cytologic slides were examined for the presence or absence of the 28 cytologic features. The cytologic criteria showing statistical significance in LGUC were increased numbers of monotonous single (non-umbrella) cells, three-dimensional cellular papillary clusters without fibrovascular cores, irregular bordered clusters, atypical single cells, irregular nuclear overlap, cytoplasmic homogeneity, increased N/C ratio, pleomorphism, nuclear border irregularity, nuclear eccentricity, elongated nuclei, and hyperchromasia (p ˂ 0...
February 22, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28225145/significant-variations-in-the-seroprevalence-of-c6-elisa-antibodies-in-a-highly-endemic-area-for-lyme-borreliosis-evaluation-of-age-sex-and-seasonal-differences
#11
Marcus Johansson, Lena Manfredsson, Annika Wistedt, Lena Serrander, Ivar Tjernberg
The purpose of this study was to assess the seroprevalence of C6 ELISA antibodies in healthy blood donors in Kalmar County, Sweden, in relation to age, sex and time of year (peak season vs off season). In addition, we wanted to assess serological status over time in a group of C6 ELISA seropositive blood donors. Sera were collected from 273 (131 women, 142 men) blood donors in autumn 2011 and 300 (144 women, 156 men) in winter 2014. All sera were analysed in the C6 ELISA and the results were interpreted according to the manufacturer's instructions...
February 22, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28211096/monocyte-and-lymphocyte-surface-molecules-in-severe-sepsis-and-non-septic-critically-ill-patients
#12
Joel Jämsä, Hannu Syrjälä, Virva Huotari, Eeva-Riitta Savolainen, Tero Ala-Kokko
The aim of the present study was to investigate whether expression of monocyte and lymphocyte surface molecules differs between patients with severe sepsis and non-septic patients treated in the intensive care unit (ICU). The expression of monocyte CD14, CD40, CD80 and HLA-DR, and lymphocyte CD69 were analyzed using quantitative flow cytometry on three consecutive days in 27 patients with severe sepsis and in 15 non-septic patients. Receiver operating characteristic analyses were performed and each corresponding area under the curve (AUC) was determined...
February 17, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28233451/immunogenicity-and-efficacy-of-a-bivalent-dna-vaccine-containing-leif-and-tsa-genes-against-murine-cutaneous-leishmaniasis
#13
Nahid Maspi, Fatemeh Ghaffarifar, Zohreh Sharifi, Abdolhossein Dalimi, Mohammad Saaid Dayer
There is no effective vaccine for the prevention and elimination of leishmaniasis. For this reason, we assessed the protective effects of DNA vaccines containing LeIF, TSA genes alone, or LeIF-TSA fusion against cutaneous leishmaniasis pEGFP-N1 plasmid (empty vector) and phosphate buffer saline (PBS) were used as control groups. Therefore, cellular and humoral immune responses were evaluated before and after the challenge with Leishmania major. Lesion diameter was also measured 3-12 weeks after challenge. All immunized mice with plasmid DNA encoding Leishmania antigens induced the partial immunity characterized by increased IFN-γ and IgG2a levels compared with control groups (p < 0...
March 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28233450/different-subtypes-of-epithelioid-sarcoma-and-their-clinical-implication-long-term-multi-institutional-experience-with-a-rare-sarcoma
#14
MULTICENTER STUDY
Chan Kim, Kwai Han Yoo, Min Hwan Kim, Hong Jae Chon, Soon Il Lee, Hyo Jin Lee, Sujin Koh, Ha Yeon Lee, Hye Ran Lee, Kyung Sik Kim, Young Deuk Choi, Sun Young Rha, Su Jin Lee, Hyo Song Kim
Epithelioid sarcoma (ES) is a very rare soft-tissue sarcoma with a high tendency of recurrence and metastasis. We analyzed clinical features of ES and aimed to identify the potential role of radio- and chemotherapy in ES. Fifty-five patients diagnosed with ES between 1997 and 2014 were enrolled from seven tertiary hospitals in Korean Cancer Research Group. The clinical variables were retrospectively reviewed and analyzed. Forty-six (84%) patients underwent surgical resection of ES, and among them, 27 experienced recurrence...
March 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28233449/iron-uptake-and-bacteriocin-genes-among-escherichia-coli-strains-from-skin-and-soft-tissue-infections
#15
LETTER
Marjanca Starčič Erjavec, Živa Petkovšek, Luka Predojević, Darja Žgur-Bertok
No abstract text is available yet for this article.
March 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28233448/vascular-invasion-is-an-adverse-prognostic-factor-in-resected-non-small-cell-lung-cancer
#16
Maria Ramnefjell, Christina Aamelfot, Lars Helgeland, Lars A Akslen
Lung cancer is a leading cause of death, and there is a need for better prognostic factors in treatment decisions. Vascular invasion is a known negative prognosticator, but it is not clear how to evaluate this feature. Here, we studied the prevalence and prognostic impact of blood and lymphatic vascular invasion (BVI, LVI), tumour grade, necrosis, inflammation and pleural invasion on cancer-specific survival (LCSS) and time to recurrence (TTR) in non-small-cell lung cancer (NSCLC). A total of 438 patients surgically treated for NSCLC (1993-2010) were examined, including 213 adenocarcinomas (AC), 135 squamous cell carcinomas (SCC) and 90 other NSCLC...
March 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28233447/carbonic-anhydrase-ii-a-novel-biomarker-for-pseudomyxoma-peritonei
#17
Petrus Järvinen, Antti J Kivelä, Pirjo Nummela, Anna Lepistö, Ari Ristimäki, Seppo Parkkila
Altered expression of carbonic anhydrase (CA) II is associated with human carcinogenesis. We analysed CA II protein expression in 89 patients with pseudomyxoma peritonei (PMP) and correlated its association against survival. We determined the expression of CA II by immunohistochemistry and then scored the staining results. The correlations of CA II expression with Peritoneal Cancer Index (PCI) and tumour grade were examined. The effect of CA II and tumour grade on survival was investigated. Positive CA II expression was found in 58 patients (65%) and absent in 31 patients (35%)...
March 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28233446/development-of-de-novo-psoriasis-during-nivolumab-therapy-for-metastatic-renal-cell-carcinoma-immunohistochemical-analyses-and-clinical-outcome
#18
LETTER
Juan Ruiz-Bañobre, Ihab Abdulkader, Urbano Anido, Luis León, Rafael López-López, Jorge García-González
No abstract text is available yet for this article.
March 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28233445/transcriptomic-and-virulence-factors-analyses-of-cryptococcus-neoformans-hypoxia-response
#19
Qingtao Kong, Rui Yang, Zhen Wang, Wenquan Zhou, Xue Du, Suyang Huang, Yuan Jiang, Weida Liu, Hong Sang
Cryptococcus neoformans is an environmental pathogen requiring atmospheric levels of oxygen for optimal growth. Upon inhalation, C. neoformans disseminates to the brain and causes meningoencephalitis. However, the mechanisms by which the pathogen adapts to the low-oxygen environment in the brain have not been investigated. We isolated a C. neoformans strain with a small capsule from a host tissue, although this strain produces large capsules in normoxic conditions. We hypothesize that this difference in capsule size is attributed to hypoxia caused by chronic inflammatory response...
March 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28233444/expression-of-erythropoietin-and-neuroendocrine-markers-in-clear-cell-renal-cell-carcinoma
#20
Patricia G Mjønes, Ivar S Nordrum, Gunnar Qvigstad, Øystein Sørdal, Liselotte Linnea Mårtensson Rian, Helge L Waldum
The aim of the study was to investigate the expression of erythropoietin and neuroendocrine markers in clear cell renal cell carcinoma (CCRCC). We retrospectively reviewed the medical records and re-evaluated histopathological specimens of 33 patients with CCRCC and compared with those of 11 cases of non-CCRCC. All patients were treated with a partial or radical nephrectomy at St. Olavs Hospital, Trondheim University Hospital, between 2010 and 2016. Thirty-three patients who were diagnosed with CCRCC had a total of 35 tumours, where 34 of the tumours were CCRCC and one was papillary adenoma...
March 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
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