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APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica

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https://www.readbyqxmd.com/read/30191621/predictive-value-of-combined-analysis-of-pro-npy-and-erg-in-localized-prostate-cancer
#1
Gitte Kristensen, Martin Andreas Røder, Kasper Drimer Berg, Johanna Elversang, Diego Iglesias-Gato, José Moreira, Birgitte Grønkaer Toft, Klaus Brasso
This study aimed to investigate if combined analysis of pro-Neuropeptide Y (NPY) and ERG expression in tumor tissue are associated with biochemical failure (BF), castration-based treatment, castration-resistant prostate cancer (CRPC), and prostate cancer (PCa)-specific death for men undergoing radical prostatectomy (RP) for PCa. This study included 315 patients, who underwent RP from 2002 to 2005. Both pro-NPY and ERG expression were analyzed using immunohistochemistry and were scored as low or high and negative or positive, respectively...
September 6, 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/30191618/performance-characteristics-of-newer-mic-gradient-strip-tests-compared-with-the-etest-for-antimicrobial-susceptibility-testing-of-neisseria-gonorrhoeae
#2
Agnez Jönsson, Susanne Jacobsson, Sunniva Foerster, Michelle J Cole, Magnus Unemo
For Neisseria gonorrhoeae susceptibility testing, Etest, comparable to agar dilution, is frequently used. In recent years, newer MIC gradient strip tests have been commercialized. However, these tests have not been appropriately evaluated for gonococci. We evaluated the sensitivity, specificity, accuracy, quality, availability of antimicrobials and cost of the MIC Test Strip (Liofilchem), M.I.C.Evaluator (Oxoid) and Ezy MIC Strip (HiMedia), compared to the reference Etest (bioMérieux), for gonococcal susceptibility testing...
September 6, 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/30191615/the-use-of-fluorescent-staining-techniques-for-microscopic-investigation-of-polymorphonuclear-leukocytes-and-bacteria
#3
REVIEW
Maria Alhede, Camilla Stavnsbjerg, Thomas Bjarnsholt
The use of fluorescent stains to visually investigate eukaryotic and/or prokaryotic cells is increasing quickly and manuscripts within all areas of research publish results using fluorescent staining techniques. However, in contrast to literature on traditional histological staining techniques, the literature on fluorescent stains and staining techniques does not offer as good an illustration of cellular morphology. The aim of this guideline is to illustrate different fluorescent stains and staining techniques for imaging immune cells, in particular the polymorphonuclear leukocytes (PMNs), in combination with infecting bacteria as seen in chronic bacterial infections...
September 6, 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/30160023/primary-and-secondary-mucosal-melanoma-of-the-small-intestine-a-clinical-pathological-and-genetic-nationwide-survey-of-danish-patients-between-1980-and-2014
#4
Jakob Koefoed Tingsgaard, Amalie Henriksen, Lauge Hjorth Mikkelsen, Nille Behrendt, Linea Cecilie Melchior, Lars Bo Svendsen, Steffen Heegaard
The aim of this study was to describe the epidemiology, symptomatology, pathology, genetics, and treatment of primary and metastatic small intestine melanoma in a national Danish cohort. All Danish patients diagnosed with small intestinal melanoma during the period 1980-2014 were included. For each patient, clinical data along with available pathology report and tissue was registered. Targeted next-generation sequencing (NGS) of known hotspots in 50 oncogenic genes was performed. Twenty patients with small intestinal melanoma were retrieved...
September 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/30160022/hypochromatic-large-urothelial-cells-in-urine-cytology-are-indicative-of-high-grade-urothelial-carcinoma
#5
Francesco Pierconti, Maurizio Martini, Patrizia Straccia, Vincenzo Fiorentino, Teresa Musarra, Luigi Maria Larocca, Antonio Lopez-Beltran
In this study, we have examined 67 cytology specimens from patients from 2014 to 2016. The ratio man to women was 4:1 with a median age of 75 years (range: 55-87 years). Thin-Prep processed urinary cytology specimens demonstrated large urothelial cells, with cytologic features of malignancy, thus including hypochromatic nuclei with occasional peripherally accentuated chromatin rim. The cytological diagnosis of High Grade Urothelial Carcinoma (HGUC) was made in 55 patients while 12 specimens were classified as Atypical Urothelial Cells (AUC)...
September 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/30160021/stability-of-oestrogen-and-progesterone-receptor-antigenicity-in-formalin-fixed-paraffin-embedded-breast-cancer-tissue-over-time
#6
Anna Ehinger, Pär-Ola Bendahl, Lisa Rydén, Mårten Fernö, Sara Alkner
Use of archived formalin-fixed paraffin-embedded (FFPE) tissue is a standard method for evaluation of proposed prognostic and predictive tumour markers. However, little is known of the preservation of biomarker expression in old FFPE tumour blocks. We investigate the quality of immunohistochemical (IHC) oestrogen (ER) and progesterone receptor (PR) evaluation in FFPE tissue over time (1978-2000) using a large breast cancer tissue microarray (N = 573) with access to receptor analyses in cytosol (CYT) at diagnosis, coexpression of other biomarkers and follow-up data...
September 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/30160020/increased-levels-of-serum-mir-148a-3p-are-associated-with-prostate-cancer
#7
Sandra Amalie Dybos, Arnar Flatberg, Jostein Halgunset, Trond Viset, Toril Rolfseng, Solveig Kvam, Haakon Skogseth
Prostate cancer (PCa) is one of the most common types of cancer and the fifth leading cause of death among men worldwide. The tools for diagnosing PCa have limited value, and to improve correct diagnosis there is a need for markers that can contribute to a more precise diagnosis, which would lead to proper treatment of only those patients who need it. Micro RNA (miRNA) plays a key role in the development of cancer and is therefore a potential marker for PCa. Next-generation sequencing was used to discover differences in miRNA expression between serum samples from PCa patients and healthy controls, and the results were validated by quantitative real-time polymerase chain reaction...
September 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/30160019/vascular-adhesion-protein-1-as-indicator-of-breast-cancer-tumor-aggressiveness-and-invasiveness
#8
Yen-Chang Clark Lai, Shu-Jyuan Chang, Joanna Kostoro, Aij-Lie Kwan, Chee-Yin Chai
Recent studies suggest that vascular adhesion protein-1 (VAP-1), a 180-KDa homodimeric glycoprotein, may be associated with cancer-related events including tumor cell migration, motility, invasion, or metastasis. Therefore, this study applies VAP-1 immunohistochemical staining to demonstrate the invasiveness component of the breast cancer. The VAP-1 staining results were compared in 148 breast cancer cases to identify possible correlations with clinical status, including age, tumor size, tumor grade, TNM stage, lymphatic invasion, metastasis, recurrence, and survival rate...
September 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/30160018/cd8-and-cd163-infiltrating-cells-and-pd-l1-immunoexpression-in-oral-leukoplakia-and-oral-carcinoma
#9
Olga Stasikowska-Kanicka, Małgorzata Wągrowska-Danilewicz, Marian Danilewicz
Overexpression of inhibitory checkpoint PD1/PD-L1 plays an important role in carcinogenesis and patients prognosis. 70 cases of oral squamous cell carcinoma (OSCC), 23 cases of oral leukoplakia (OLK), and 19 control cases were immunohistochemically stained with anti-PD-L1, -CD8, and -CD163 antibodies. PD-L1 was expressed on dysplastic and subepithelial infiltrating cells of OLK as well as on cancer and tumor-infiltrating cells of OSCC. In OSCC, PD-L1 immunoexpression was significantly increased in comparison to OLK, and control groups...
September 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/30160017/survival-of-campylobacter-jejuni-and-campylobacter-coli-water-isolates-in-lake-and-well-water
#10
Anna Nilsson, Cecilia Johansson, Astrid Skarp, René Kaden, Stefan Bertilsson, Hilpi Rautelin
The role of water for transmission of Campylobacter jejuni and C. coli to humans might be underestimated, as factors important for bacterial viability in water are largely unknown. We have studied water survival of seven C. jejuni and eight C. coli isolates originally isolated from Swedish waters, together with selected reference strains, over eight days at 4 °C in the dark in untreated water collected from a local lake and a private well. To study seasonality, lake water samples were collected during spring and autumn...
September 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/30160016/unicystic-high-grade-intraductal-carcinoma-of-the-parotid-gland-cytological-and-histological-description-with-clinic-pathologic-review-of-the-literature
#11
REVIEW
Andrea Palicelli, Paola Barbieri, Narciso Mariani, Paola Re, Stefania Galla, Raffaele Sorrentino, Francesca Locatelli, Nunzio Salfi, Guido Valente
Intraductal carcinoma of the salivary glands is a rare, not well-characterized tumor. We reviewed the literature and report the first case of a high-grade unicystic intraductal carcinoma of the parotid. Formalin-fixed/paraffin-embedded blocks were sectioned and stained for hematoxylin and eosin and immunostains (CAM5.2, EMA, CK5, p53, p63, SMA, S100 protein, DOG1, mammaglobin, AR, ER, PR, Her-2, and Ki67). A 72-year-old man showed a painless nodule (2 cm) in the right parotid region. A 'tumor of uncertain malignant potential' (low grade) was diagnosed by fine-needle aspiration cytology (FNAC)...
September 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/30058214/hepatoprotectivity-of-panduratin-a-against-liver-damage-in-vivo-demonstration-with-a-rat-model-of-cirrhosis-induced-by-thioacetamide
#12
Suzy M Salama, Ibrahim Abdel Aziz Ibrahim, Naiyer Shahzad, Saeed Al-Ghamdi, Nahla Ayoub, Ahmed Salim AlRashdi, Mahmood Ameen Abdulla, Nur'Ain Salehen, Mehmet Bilgen
This experiment evaluated Panduratin A (PA), a chalcone isolated from Boesenbergia rotunda rhizomes, for its hepatoprotectivity. Rats were subjected to liver damage induced by intra-peritoneal injection of thioacetamide (TAA). PA was tested first for its acute toxicity and then administered by oral gavage at doses 5, 10, and 50 mg/kg to rats. At the end of the 8th week, livers from all rats were excised and evaluated ex vivo. Measurements included alkaline phosphatase (AP), alanine transaminase (ALT), aspartate transaminase (AST) and gamma-glutamyl transferase (GGT), serum platelet-derived growth factor (PDGF) and transforming growth factor (TGF-β1), and hepatic metalloproteinase enzyme (MMP-2) and its inhibitor extracellular matrix protein (TIMP-1)...
September 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/30168625/evaluation-of-the-gastrointestinal-tract-in-mdx-mice-an-experimental-model-of-duchenne-muscular-dystrophy
#13
David Feder, Mariana Ierardi, Ana Laura Covre, Giuliana Petri, Alzira Alves de Siqueira Carvalho, Fernando Luiz Affonso Fonseca, Bruno Machado Bertassoli
This study describes the functional and morphological alterations in the intestines of mdx mice (n = 4) compared with the intestinal features of C57BL/10 mice (n = 7) at 2 months of age. The whole gut transit time (carmine red) and the upper gut transit time (activated charcoal) were measured, and light microscopy was utilized to view stained sections (H&E and picrosirius red) for histological analysis. No significant difference in mean evacuation time for the whole gut was observed between the two groups, but a significant delay in activated charcoal passage was observed in the mdx mice...
August 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/30168624/porphyromonas-gingivalis-induced-inflammatory-responses-in-thp1-cells-are-altered-by-native-and-modified-low-density-lipoproteins-in-a-strain-dependent-manner
#14
Kartheyaene Jayaprakash, Isak Demirel, Hazem Khalaf, Torbjörn Bengtsson
Strong epidemiological evidence supports an association between cardiovascular and periodontal disease and furthermore, the periodontopathogen Porphyromonas gingivalis has been identified in blood and from atheromatous plaques. Blood exposed to P. gingivalis shows an increased protein modification of low-density lipoprotein (LDL). In this study, we investigate the inflammatory responses of THP1 cells incubated with P. gingivalis and the effects of native or modified LDL on these responses. Reactive oxygen species (ROS) and IL-1β were observed in THP1 cells following infection with P...
August 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/30168623/staphylococcus-epidermidis-from-prosthetic-joint-infections-induces-lower-il-1%C3%AE-release-from-human-neutrophils-than-isolates-from-normal-flora
#15
Emeli Månsson, Bo Söderquist, Åsa Nilsdotter-Augustinsson, Eva Särndahl, Isak Demirel
The aim of this study was to test the hypothesis that Staphylococcus epidermidis isolated from prosthetic joint infections (PJIs) differs from S. epidermidis isolated from normal flora in terms of its capacity to induce activation of caspase-1 and release of IL-1β in human neutrophils. The amount of active caspase-1 was determined over 6 h by detecting Ac-YVAD-AMC fluorescence in human neutrophils incubated with S. epidermidis isolates from PJIs (ST2) or normal flora. The amount of IL-1β was detected by ELISA in neutrophil supernatants after 6 h of incubation...
August 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/30168622/patient-characteristics-of-olfactory-neuroblastoma-experience-from-a-tertiary-cancer-centre-2000-2016-covering-eastern-denmark
#16
Signe Buhl Gram, Christian Grønhøj, Henriette Mann, Kathrine Kronberg Jakobsen, Katalin Kiss, Anders Bilde, Christian von Buchwald
The aim of this study was to report incidence and patient characteristics of olfactory neuroblastoma (ONB) at a tertiary cancer institution during a 16-year period. A retrospective review was conducted on patients with ONB treated at Rigshospitalet, Copenhagen from 2000 to 2016 covering Eastern Denmark. Patient demographics, symptoms, stage, pathology-reports, treatment, and outcome were extracted from the patient records and the Danish pathology register. The tumours were graded both histologically and clinically using Hyam's and Kadish classifications, respectively...
August 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/30168621/detection-of-extended-spectrum-beta-lactamase-production-in-enterobacteriales-from-patients-with-suspected-urinary-tract-infections-tabora-region-rural-tanzania
#17
LETTER
David Nestor, Bo-Eric Malmvall, Yohana Paul Masonda, John Msafiri, Martin Sundqvist
No abstract text is available yet for this article.
August 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/29962006/implants-induce-a-new-niche-for-microbiomes
#18
MULTICENTER STUDY
Tim H Jakobsen, Steffen R Eickhardt, Alexandra G Gheorghe, Charlotte Stenqvist, Majken Sønderholm, Camilla Stavnsberg, Peter Ø Jensen, Anders Odgaard, Marvin Whiteley, Claus Moser, Jesper Hvolris, Hans Petter Hougen, Thomas Bjarnsholt
Although much work is being done to develop new treatments, research and knowledge regarding factors underlying implant-related microbial colonization leading to infection are less comprehensive. Presence of microorganisms in and around implants clinically characterized as uninfected remains unknown. The objective of this study was to detect and identify bacteria and fungi on implants from various groups of patients with no prior indications of implant related infections. Patient samples (implants and tissue) were collected from five different hospitals in the Capital region of Denmark...
August 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/29920782/lamp-1-gene-is-overexpressed-in-high-grade-glioma
#19
Victoria S Sarafian, Ilian Koev, Nikolay Mehterov, Maria Kazakova, Krassimir Dangalov
High-grade gliomas (HGG) are the most frequent brain tumors in adults. Glioblastoma multiforme (GBM) is their most aggressive form resistant to therapy. It was shown that inhibition of autophagy reduced GBM development and autophagy interfering agents are regarded as a new strategy to fight glioma cells. The lysosome-associated membrane proteins (LAMPs) display differential expression particularly in cancer. There are few data on their expression and especially on their molecular profile. The aim of the present study is to investigate the expression of LAMP-1 and LAMP-2 genes and proteins in HGG...
August 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/30129134/apmis-special-issue-placental-pathology
#20
EDITORIAL
Tina Elisabeth Olsen, Lisa Leth Maroun
No abstract text is available yet for this article.
July 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
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