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Journal of Clinical Laboratory Analysis

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https://www.readbyqxmd.com/read/28719009/diagnosis-of-chronic-heart-failure-by-the-soluble-suppression-of-tumorigenicity-2-and-n-terminal-pro-brain-natriuretic-peptide
#1
Xiao-Ling Jin, Ning Huang, Hui Shang, Ming-Cheng Zhou, Yi Hong, Wen-Zheng Cai, Jie Huang
OBJECTIVE: Our study was to explore the roles between serum soluble suppression of tumorigenicity 2 (sST2) and N-terminal pro-brain natriuretic peptide (NT-proBNP) while evaluating ventricular function to properly diagnose chronic heart failure (CHF). METHODS: In total, 197 CHF patients were recruited and classified into ventricular function's II, III, and IV groups, and 106 healthy people into normal control group. To detect concentrations of Sst2 and NT-proBNP, ELISA and electro-chemiluminescence immuno assay were implemented...
July 18, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28718958/multiplex-pcr-scheme-for-variant-plasmid-mediated-class-c-%C3%AE-lactamase-typing
#2
Le Van Chuong, Virapong Prachayasittikul, Chartchalerm Isarankura Na Ayudhya, Ratana Lawung
BACKGROUND: An increasing of prevalence and diversification of plasmid-mediated AmpC (pAmpC) has been emerged worldwide. The incidence of pAmpC resulted in increasing β-lactamase production and conferred resistance to almost all β-lactam antibiotics excluding carbapenems. The lack of standard method for pAmpC identification and classification exert a challenge in epidemiological surveillance and infection control practices. METHODS: A robust, single tube multiplex PCR has been developed to classify six different pAmpC groups including CIT (CMY-2 like, LAT and CFE), ECB (ACT, MIR), MOX & CMY-1 like, DHA, ACC, and FOX...
July 18, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28703354/identification-of-sequence-polymorphisms-in-the-mitochondrial-cytochrome-c-oxidase-genes-as-risk-factors-for-hepatocellular-carcinoma
#3
Hongfang Wang, Jinsheng Xu, Demao Li, Shenglei Zhang, Zhanjun Guo
BACKGROUND: Single nucleotide polymorphisms (SNPs) accumulated in the mitochondrial DNA (mtDNA) is susceptible to the tumor formation. We discovered previously that SNPs in the mitochondrial displacement loop (D-loop) was associated with the risk of hepatocellular carcinoma (HCC). METHODS: The cytochrome c oxidase (COX) genes of mtDNA were sequenced between 107 HCC patients and 100 matched healthy controls. The χ(2) test was used to analyze single SNPs' statistical difference between HCC patients and healthy controls...
July 13, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28703342/evaluation-of-direct-antifungal-susceptibility-testing-methods-of-candida-spp-from-positive-blood-culture-bottles
#4
Yasemin Oz, Egemen Gokbolat
BACKGROUND: Blood culture is the gold standard diagnostic method in candidemia in spite of long result time and low sensitivity rate. Early diagnosis is crucial for management of candidemia because a delay in treatment is related with increased mortality. We aimed to evaluate the direct applicability of antifungal susceptibility testing methods from positive blood culture bottles to save at least 24 hours. METHODS: Blood culture bottles were inoculated with 62 Candida isolates...
July 13, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28703321/overexpression-mir-211-5p-hinders-the-proliferation-migration-and-invasion-of-thyroid-tumor-cells-by-downregulating-sox11
#5
Lei Wang, Yan-Feng Shen, Zhi-Min Shi, Xiao-Juan Shang, Dong-Ling Jin, Feng Xi
PURPOSE: This study was aimed to investigate the relationship between miR-211-5p and SOX11, and the effects of their interaction on the proliferation, viability, and invasion of human thyroid cancer (TC) cells. METHODS: We used quantitative real-time PCR (qRT-PCR) to determine the expression of miR-211-5p and SOX11mRNA in the thyroid tumorous and the adjacent tissues. The target relationship between miR-211-5p and SOX11 was confirmed using dual luciferase reporter gene assay...
July 13, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28703296/correlation-between-tlr2-tlr3-tlr4-and-tlr9-polymorphisms-and-susceptibility-to-and-prognosis-of-severe-hepatitis-among-the-newborns
#6
Xiao Qiu, Yubin Dong, Yaqin Cao, Yingmei Luo
BACKGROUND: This study was aimed to explore how toll-like receptor 2 (TLR2), TLR3, TLR4 and TLR9 influenced the risk and prognosis of severe hepatitis among the Chinese newborns. METHODS: Altogether 135 newborns diagnosed with severe hepatitis and 140 healthy newborns were included in this study. Totally 12 single nucleotide polymorphisms (SNPs) within TLR2, TLR3, TLR4, and TLR9 were chosen and genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)...
July 13, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28670719/association-between-clinical-characteristics-and-the-diagnostic-accuracy-of-circulating-single-molecule-amplification-and-resequencing-technology-on-detection-epidermal-growth-factor-receptor-mutation-status-in-plasma-of-lung-adenocarcinoma
#7
Chao Shi, Yan Zheng, Yin Li, Haibo Sun, Shilei Liu
BACKGROUND: Lung cancer is the leading cause of cancer-related mortality in the world. Circulating single-molecule amplification and resequencing technology (cSMART) can successfully detect epidermal growth factor receptor (EGFR) mutation in non-small cell lung cancer (NSCLC). However, few studies have investigated the association between clinical characteristics and the diagnostic accuracy of cSMART technique in lung adenocarcinoma. METHODS: We enrolled 95 patients, which included paraffin embedded tumor tissues and matched plasma samples...
July 3, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28670691/the-performance-of-t-cell-xtend-reagent-in-increasing-blood-storage-times-for-interferon-gamma-release-assays
#8
Junlian Li, Yan Liu, Liyamu Ma, Libike Gu, Quan Wang, Miao Xu, Ruiying Ma, Yali Zhang, Zhenping Yang, Junjie Deng, Xing Yi
BACKGROUND: T-cell Xtend (TCX) was introduced to extend the blood storage time for T-SPOT.TB test, a widely used commercial interferon gamma release assay (IGRA) for rapid in vitro tuberculosis. METHODS: A total of 99 Uyghur suspected tuberculosis patients were recruited in this study. T-SPOT.TB test was performed with fresh blood (controls), 36 hours delayed blood and delayed and TCX-treated (at 36 hours) blood from each patient, respectively. RESULTS: White blood cells and lymphocytes proportion in peripheral blood mononuclear cells s and spot-forming cells in positive control wells decreased significantly in delayed blood samples when compared with controls, while this decrease was not detected in TCX-treated group...
July 3, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28665527/dna-decontamination-methods-for-internal-quality-management-in-clinical-pcr-laboratories
#9
Yingping Wu, Jianyong Wu, Zhihui Zhang, Chen Cheng
BACKGROUND: The polymerase chain reaction (PCR) technique, one of the most commonly applied methods in diagnostic and molecular biology, has a frustrating downside: the occurrence of false-positive signals due to contamination. In previous research, various DNA decontamination methods have been developed to overcome this limitation. Unfortunately, the use of random or poorly focused sampling methods for monitoring air and/or object surfaces leads to the incomplete elimination during decontamination procedures...
June 30, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28657176/an-improved-pcr-ctpp-assay-for-the-detection-of-adh1b-arg48his-polymorphism
#10
Shupeng Jiang, Yongqing Tong, Rui Zhao, Ge Xiong, Bin Qiao, Yan Li
BACKGROUND: ADH1B Arg48His polymorphism is associated with the development of alcohol-related diseases. In this study, we aimed to explore an improved polymerase chain reaction with confronting two-pair primers (PCR-CTPP) assay for the detection of ADH1B Arg48His polymorphism. METHODS: A mismatch was introduced at the 3' end of each of the two allele-specific to increase the specificity of the reaction. But beyond that, a new mismatch at-3 positions of outer primers was designed to decrease the efficiency of the aforementioned primers and depresses the amplification of an internal nonspecific DNA control...
June 28, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28657153/diagnostic-reliability-of-architect-anti-hcv-assay-experience-of-a-tertiary-care-hospital-in-india
#11
Gnanadurai John Fletcher, Anantharam Raghavendran, Jayashree Sivakumar, Prasanna Samuel, Priya Abraham
BACKGROUND & AIMS: Anti-HCV assays are prone to false positive results. Thus, accurate detection of HCV infection is critical for the timely therapeutic management. This study ascertained the reliability of Architect anti-HCV assay (Abbott) and to estimate the agreement of this assay with Ortho HCV 3.0 ELISA Test System with Enhanced SAVe (Ortho), HCV Tri-dot (Tri-dot) and HCV-PCR in a tertiary care setting. METHODS: A total of 78 788 consecutive sera were routinely screened for anti-HCV antibodies using Architect...
June 28, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28653772/serum-carcinoembryonic-antigen-is-positively-associated-with-leukocyte-count-in-korean-adults
#12
Yu-Jin Kwon, Hye-Sun Lee, Jae-Yong Shim, Yong-Jae Lee
BACKGROUND: Emerging evidence shows that serum carcinoembryonic antigen (CEA) levels may modestly be increased in non-neoplastic conditions such as cardiometabolic diseases, which are increasingly being seen as inflammatory diseases. Leukocyte count is widely evaluated marker of inflammation in clinical practice and a useful predictor of cardiometabolic disease. In this study, we aimed to determine the relationship between serum CEA levels and leukocyte counts in Korean adults. METHODS: This cross-sectional study included a total of 19 834 individuals enrolled from a health promotion center between November 2006 and July 2010...
June 27, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28650079/a-retrospective-analysis-of-the-application-of-the-elecsys-%C3%A2-hiv-combi-pt-assay-in-southern-china
#13
Shaocong Liang, Guihua Deng, Shaosong Zhou, Jing Zeng, Weiqing Tan, Xiaopeng Yuan
BACKGROUND: Fourth-generation HIV assays have been implemented worldwide as a screening test for many years. Understanding the performance of fourth-generation assay in low HIV prevalence region is pivotal to interpret the test result correctly. In this study, retrospective analysis was used to evaluate application of the Elecsys(®) HIV combi PT assay. METHODS: A total of 85 043 specimens from a low prevalence setting were detected between June 2013 and October 2015...
June 26, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28649781/anti-d-reagents-should-be-chosen-accordingly-to-the-prevalence-of-d-variants-in-the-obstetric-population
#14
Jelena Lukacevic Krstic, Slavica Dajak, Jasna Bingulac-Popovic, Vesna Dogic, Jela Mratinovic-Mikulandra
BACKGROUND: Resolving ambiguous results of D antigen typing is crucial for appropriate and rational administration of anti-D immunoprophylaxis and transfusion practice in obstetric population. The aim of the study was to establish selection criteria of anti-D reagents for our population. METHODS: A total of 12 689 samples from primiparous women in Split-Dalmatia County, Croatia, were typed for RhD antigen during the period of 5 years. Ambiguous results were submitted to additional serologic investigation and genotyping...
June 26, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28649724/evaluation-of-the-uriscan-2-acr-strip-to-estimate-the-urine-albumin-creatinine-ratios
#15
Sangeun Lim, Hui-Jin Yu, Seungjun Lee, Hyosoon Park, Min-Jung Kwon, Hee-Yeon Woo
BACKGROUND: The urine albumin/creatinine ratio (ACR) test is used to screen patients with chronic diseases, such as diabetes, hypertension and cardiovascular diseases that put them at an increased risk of developing kidney disease. Here, we evaluated the performance of the URiSCAN 2 ACR Strip (URiSCAN; YD diagnostics, Yongin, Korea), a semiquantitative point-of-care testing (POCT) assay, and we compared to an existing POCT assay and a quantitative assay. MATERIALS AND METHODS: A total of 1,020 random urine specimens were analyzed using the semiquantitative URiSCAN 2 ACR Strip and semiquantitative CLINITEK Microalbumin 2 Strip (CLINITEK; Siemens, New York, USA)...
June 26, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28649705/naoh-hcl-neutralized-urine-preparation-for-direct-testing-of-uropathogens-by-bruker-ms
#16
Ji Seon Choi, Jayoung Kim, Han-Soo Kim, Chang-Wan Kwon, Min-Sung Kim, Seong-Hyeok Choi
BACKGROUND: We evaluated the analytical performance of a NaOH-HCl neutralization protocol for identifying uropathogens directly from urine samples by a Bruker MS system (Bruker Daltonics, German) and sought to establish a protocol for integrating the method with conventional screening. METHODS: Among all urine samples requested for Gram staining, UF-1000i, and urine cultures by physicians, we selected samples that were positive by both Gram staining and UF-1000i testing...
June 26, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28643405/reassessment-of-the-access-testosterone-chemiluminescence-assay-and-comparison-with-lc-ms-method
#17
Ruggero Dittadi, Mara Matteucci, Elisa Meneghetti, Rudina Ndreu
AIM OF THE STUDY: To reassess the imprecision and Limit of Quantitation, to evaluate the cross-reaction with dehydroepiandrosterone-sulfate (DHEAS), the accuracy toward liquid chromatography-mass spectrometry (LC-MS) and the reference interval of the Access Testosterone method, performed by DxI immunoassay platform (Beckman Coulter). MATERIAL AND METHODS: Imprecision was evaluated testing six pool samples assayed in 20 different run using two reagents lots. The cross-reaction with DHEAS was studied both by a displacement curve and by spiking DHEAS standard in two serum samples with known amount of testosterone...
June 23, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28643361/screening-for-del-phenotype-in-rhd-negative-indians
#18
Swati Kulkarni, Disha S Parchure, Vidya Gopalkrishnan, Manisha Madkaikar
BACKGROUND: DEL phenotype represents a very weak form of D variant detected only by adsorption and elution technique. DEL phenotype individuals mistyped as RhD-negative can lead to alloimmunization after transfusion or pregnancy. Molecular techniques have now been used to identify DEL variants. They are commonly encountered in the East Asian population with RHD(K409K) being the most frequent allele. RHD(M295I) is the most common DEL allele in Caucasians. As there is a paucity of data on DEL phenotype in the Indian population, the study aims to screen RhD negative individuals for two most common DEL mutations...
June 23, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28643351/quality-specifications-of-routine-clinical-chemistry-methods-based-on-sigma-metrics-in-performance-evaluation
#19
Jun Xia, Su-Feng Chen, Fei Xu, Yong-Lie Zhou
INTRODUCTION: Sigma metrics were applied to evaluate the performance of 20 routine chemistry assays, and individual quality control criteria were established based on the sigma values of different assays. METHODS: Precisions were expressed as the average coefficient variations (CVs) of long-term two-level chemistry controls. The biases of the 20 assays were obtained from the results of trueness programs organized by National Center for Clinical Laboratories (NCCL, China) in 2016...
June 23, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28643348/the-construction-of-a-panel-of-serum-amino-acids-for-the-identification-of-early-chronic-kidney-disease-patients
#20
Rui Li, Jinna Dai, Hui Kang
BACKGROUND: Serum creatinine, urea, and cystatin-c are standardly used for the evaluation of renal function in the clinic. However, some patients have chronic kidney disease but still retain kidney function; a conventional serum index in these patients can be completely normal. Serum amino acid levels can reflect subtle changes in metabolism and are closely related to renal function. Here, we investigated how amino acids change as renal impairment increases. METHODS: Subjects were divided into three groups by renal function glomerular filtration rate: healthy controls, patients with chronic kidney disease with normal kidney function, and patients with chronic kidney disease with decreased kidney function group...
June 23, 2017: Journal of Clinical Laboratory Analysis
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