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Journal of Clinical Laboratory Analysis

Irena Druce, Hussein Abujrad, Seham Chaker, Hilary Meggison, Andrew Hill, Angela Raymond, Janice Mayne, Teik Chye Ooi
BACKGROUND: A decrease in serum low-density lipoprotein cholesterol (LDL-C) is well documented after acute stress. Plasma proprotein convertase subtilisin kexin 9 (PCSK9), which promotes degradation of low-density lipoprotein receptor (LDL-R) resulting in reduced plasma clearance of low-density lipoproteins (LDL) and an increase in serum LDL-C, would be predicted to decrease. Yet, a few studies have demonstrated an increase 1-8 days after acute stress. Our objective was to assess the earlier status of plasma PCSK9, within the first 24 hours of onset of stress...
November 17, 2017: Journal of Clinical Laboratory Analysis
Eun-Jung Cho, Dae-Hyun Ko, Woochang Lee, Sail Chun, Hae-Kyung Lee, Won-Ki Min
BACKGROUND: Therapeutic monitoring of tacrolimus is essential for reducing organ rejection and adverse effects. The measurement of tacrolimus in whole blood is taken by many automated platforms. We evaluated the analytical performance of the Dimension TAC assay, which is an upgraded reagent from the previous Dimension TACR assay. METHODS: The evaluations involved determination of precision, linearity, detection capability, and reagent lot-to-lot variability between three lot numbers...
November 17, 2017: Journal of Clinical Laboratory Analysis
Zhiwu Dong, Qiang Guo, Li Sun, Feifei Li, Aihong Zhao, Jingfan Liu, Peipei Qu, Qinghua Zhu, Chunhai Xiao, Fusheng Niu, Shuang Liang
OBJECTIVE: This study aims to determine the risk factors and to predict the occurrence of cerebral infarction in patients with carotid artery stenosis. METHODS: Two hundred and one subjects with carotid artery stenosis were retrospectively selected from Jinshan Branch of Shanghai Sixth People's Hospital, 115 cases of which with cerebral infarction and 86 without it. Clinical tests were performed including coagulation indices, fasting glucose, serum lipid, and blood rheology...
November 11, 2017: Journal of Clinical Laboratory Analysis
Zahra Jafari Giv, Amir Avan, Farshid Hamidi, Maryam Tayefi, Sayyed Saeid Khayyatzadeh, Ali Javandoost, Mohsen Nematy, Gordon A Ferns, Majid Ghayour Mobarhan
INTRODUCTION: Dietary calcium and phosphorus appear to be important determinants of dyslipidemia and hypertension. We have investigated (1) the prevalence of hypocalcemia and hypophosphatemia in an Iranian population, and (2) the association between nutrient's intake and serum levels of calcium and phosphorus. METHODS: A total of 908 individuals were enrolled using a stratified-cluster sampling method from the Mashhad stroke and heart atherosclerosis disorder (MASHHAD) study...
November 7, 2017: Journal of Clinical Laboratory Analysis
Lican Han, Wei Zheng, Yanhong Zhai, Xin Xie, Jingnan Zhang, Shaozu Zhang, Zhen Zhao, Zheng Cao
BACKGROUND: As a result of physiological and metabolic changes during pregnancy, thyroid hormones can be affected significantly throughout entire three trimesters. According to the guidelines published by American Thyroid Association in 2017, it is strongly recommended to establish population-based trimester-specific and assay method-specific reference intervals (RIs) using local population. METHODS: A total of 1209 pregnant women without personal or family history of thyroid disease were recruited from July 2015 to April 2017 at Beijing Obstetrics and Gynecology Hospital...
November 4, 2017: Journal of Clinical Laboratory Analysis
Ye-Long Chen, Li-Hong Shou, Zong-Xin Zhang
OBJECTIVE: We aim to explain the correlation among IL-18 gene polymorphism, its protein expression and LEDVT in the Chinese Han population. METHODS: A total of 138 LEDVT patients and 150 healthy people volunteered as LEDVT and control groups. All the data, including the gender, age, BMI, levels of TG, LDL/HDL, TC, GLU, APTT, BUN, Cr, ALT, AST, ApoA1, ApoB, and Fg was detected. IL-18 level, IL-18 -137G/C and -607C/A polymorphism, and risk factors of LEDVT were detected using ELISA, PCR-RFLP and multivariate logistic regression analysis, respectively...
November 4, 2017: Journal of Clinical Laboratory Analysis
Alireza Esmailzadeh, Hossein Zarrinfar, AbdolMajid Fata, Tanuka Sen
BACKGROUND: Among the fungal pathogens, Candida species are the most common cause of urinary tract infection (UTI). Some predisposing factors such as diabetes mellitus, urinary retention, urinary stasis, renal transplantation, and hospitalization can increase the risk of candiduria. The aim of this cross-sectional study was to evaluate candiduria among type 2 diabetic patients and identification of the Candida isolates. METHOD: Four hundred clean-catch midstream urine specimens were obtained from patients with type 2 diabetes mellitus...
October 27, 2017: Journal of Clinical Laboratory Analysis
Senem Alkan Ozdemir, Esra Arun Ozer, Ozkan Ilhan, Sumer Sutcuoglu
BACKGROUND: The neutrophil to lymphocyte ratio (NLR) is an easily accessible biomarker that has been reported to represent disease severity in adult trials. The aim of this study was to evaluate the relationship between culture positiveness and NLR in cases where the reason of sepsis was considered, and to foresight an idea about the active agents. METHODS: Preterm infants with birth weights ≤1500 g and/or ≤32 gestational weeks were eligible for this study...
October 21, 2017: Journal of Clinical Laboratory Analysis
Yu Liu, Wenhui Wan, Fang Fang, Lei Guo, Yusheng Zhao, Xinghu Zhang, Fang Huang
BACKGROUND: Peroxisome proliferator-activated receptor-γ (PPARγ) is a regulator of inflammation. This study aimed to explore associations between PPARγ gene single-nucleotide polymorphisms (SNPs) and susceptibility to and clinical outcome of sepsis in the North China Han population. METHODS: This study included 303 patients with sepsis and 303 controls. We conducted genetic typing for 13 common PPARγ gene SNPs (improved multiplex ligation detection reaction), linkage disequilibrium mapping, and haplotype inference...
October 21, 2017: Journal of Clinical Laboratory Analysis
Yongfu Shao, Rongdan Lu, Yunben Yang, Qiancheng Xu, Bojun Wang, Guoliang Ye
BACKGROUND: Resistance of Helicobacter pylori (H. pylori) to antibiotics is increasing worldwide. To determine the status of H. pylori resistance and its patterns in clinical patients, an investigation utilizing susceptibility testing for commonly used antibiotics was needed. METHODS: Total of 2283 H. pylori strains were collected from 2013 to 2016. The resistance and its patterns of these strains were tested by agar dilution method. The resistance rate and minimal inhibition concentration (MIC) in different gender groups were also analyzed...
October 6, 2017: Journal of Clinical Laboratory Analysis
Matsuo Deguchi, Masanori Kagita, Nori Yoshioka, Hiroko Tsukamoto, Miyuki Takao, Kazuko Tahara, Ikuhiro Maeda, Yoh Hidaka, Satoshi Yamauchi, Atsushi Kaneko, Hideo Miyakoshi, Mitsuo Isomura
BACKGROUND: Ongoing efforts in the development of HBsAg detection kits are focused on improving sensitivity and specificity. The purpose of this study was to evaluate an improved, highly sensitive quantitative assay, "Lumipulse HBsAg-HQ", a chemiluminescent enzyme immunoassay designed for a fully automated instrument, the "Lumipulse G1200". METHODS: Serum samples for reproducibility, dilution, correlation, sensitivity, and specificity studies were obtained from patients at the Osaka University Hospital...
October 6, 2017: Journal of Clinical Laboratory Analysis
Hao Li, Yong Cai, An-Ding Xu
AIMS: To investigate the impact of 4 single nucleotide polymorphisms (SNPs) within ABO gene and their gene-gene interactions on ischemic stroke (IS) susceptibility in Chinese Han population. METHODS: A total of 1993 participants (1375 males, 618 females) were selected, including 991 IS patients and 1002 normal controls. The SNPstats ( was used for Hardy-Weinberg equilibrium (HWE) test. Generalized multifactor dimensionality reduction (GMDR) was used to screen the best interaction combination among 4 SNPs within ABO gene...
October 6, 2017: Journal of Clinical Laboratory Analysis
Mirna Giselle Moreira, Lidia Miranda Barreto, Vera Lúcia Dos Santos, Andrea Souza Monteiro, Vandack Nobre, Simone Gonçalves Dos Santos
BACKGROUND: New Delhi Metallo-b-lactamase (NDM-1) is an enzyme emerging around the world conferring resistance to a wide range of β-lactams agents and whose early detection is extremely important. We proposed to standardize the detection of the blaNDM-1 gene using the LOOP-mediated isothermal amplification technique (LAMP). METHODS: In all, 14 Gram-negative bacterial strains isolated from patients presenting pneumonia associated with mechanical ventilation were used for the blaNDM-1 standardization by LAMP...
September 28, 2017: Journal of Clinical Laboratory Analysis
Hui Wang, Xiaoxin Sun, Wanli Dong, Xiuying Cai, Yun Zhou, Youtao Zhang, Weijian Jiang, Qi Fang
OBJECTIVE: This study aimed to explore the association between GPIa, COX-2 gene polymorphisms and aspirin resistance in the ischemic stroke patients from the southern part of Jiangsu province. METHODS: In all, 97 patients with acute ischemic stroke were enrolled in the study. GPIa gene polymorphism at 807C>T (rsl126643) locus and COX-2 gene polymorphism at -765G>C (rs20417) locus were genotyped by PCR pyrosequencing technology. Patients were divided into the aspirin sensitivity (AS) group and aspirin resistance (AR) group according to the platelet aggregation rate...
September 26, 2017: Journal of Clinical Laboratory Analysis
Elise Schapkaitz, Phoyisile Khoza
BACKGROUND: Automated hematology analyzers generate white blood cell (WBC) suspect flags to indicate smears requiring manual review. In a human immunodeficiency virus (HIV) patient population with a high frequency of abnormal specimens, the usefulness of these flags depends on their sensitivity and specificity. The white cell precursor (WPC) channel on the Sysmex XN-9000 analyzer (Sysmex Corporation, Kobe, Japan) has recently been introduced in order to distinguish immature cells such as blasts and abnormal lymphocytes...
September 26, 2017: Journal of Clinical Laboratory Analysis
Justyna Gornowicz-Porowska, Agnieszka Seraszek-Jaros, Monika Bowszyc-Dmochowska, Paweł Bartkiewicz, Elżbieta Kaczmarek, Marian Dmochowski
BACKGROUND: Technical innovation of autoimmune blistering dermatoses (ABDs) diagnosis aimed at multiplex approach. Two multiparametric ELISA tests are commercially available for ABDs serology. The aim was to compare diagnostic accuracy of multiparametric and monospecific ELISAs and to examine the diagnostic value/agreement of multivariant ELISA in compliance with traditional diagnostic setup for ABDs. METHODS: In total, 128 sera from suspected ABDs patients were studied (27 sera in order to compare ELISAs)...
September 26, 2017: Journal of Clinical Laboratory Analysis
Sana Sahengbieke, Jian Wang, Xiangwei Li, Yuhong Wang, Maode Lai, Jingjing Wu
BACKGROUND: Detection of circulating cell-free mRNA serves as noninvasive tools for cancer diagnosis. As an oncofetal protein, HMGA2 (high mobility group AT-hook 2) is upregulated in colorectal cancer (CRC) tissues. However, it is not clear whether the increased levels of circulating cell-free HMGA2 mRNA functions as potential biomarkers for improved diagnosis of CRC. METHODS: To assess its clinical significance in diagnosis and prediction, we evaluated serum levels of circulating HMGA2 mRNA in CRC patients and in healthy controls...
September 26, 2017: Journal of Clinical Laboratory Analysis
Jie Yan, Donghong Deng, Peng Cheng, Lin Liao, Meiling Luo, Faquan Lin
BACKGROUND: Dysfibrinogenemia is a rare coagulation disorder caused by mutations in the fibrinogen gene that results in abnormal fibrinogen function. Dysfibrinogenemia has a wide spectrum of clinical manifestations including asymptomatic(55%), hemorrhage (25%), and thrombosis (20%). METHODS: We reported a 30-year-old woman with 35 weeks gestation. She was misdiagnosed with hypofibrinogenemia in a local hospital, and then she was treated with fibrinogen concentrate...
September 26, 2017: Journal of Clinical Laboratory Analysis
Jingkun Wang, Yizhen Fang, Peihua Li, Huayue Lin, Hongwei Jin, Lei Gao, Shengxiang Ge, Zhong-Ying Zhang
BACKGROUND: To evaluate the performance of a chemiluminescence detection kit for cardiac troponin T (cTnT). METHODS: According to the "Guiding principles on performance analysis of diagnostic reagents in vitro" and the Clinical and Laboratory Standards Institute (CLSI) Guidelines, we assessed the detection limit, linear range, reportable range, accuracy, precision, cross reactivity, interference factors, and matrix effect of the kit, and compared these parameters with that of the commercial electrochemiluminescence detection kit for cTnT (Roche)...
September 22, 2017: Journal of Clinical Laboratory Analysis
Hong-Wei Yu, Ze Zhang, Jing-Hui Jiang, Hong-Zhi Pan, Dong Chang
BACKGROUND: Cellular and brain metabolism of dopamine can be correlated with a number of neurodegenerative disorders, our study was to explore a simple and efficient method to detect dopamine in real samples. METHODS: A new quantum dots (CdTe QDs) could be prepared using the hydrothermal method, the electrochemical biosensor was established by dropping CdTe QDs on the surface of glassy carbon electrode (GCE). RESULTS: The CdTe QDs/GCE exhibited the excellent electrochemical catalytic activity toward dopamine (DA) with good stability and high sensitivity in presence of interfering substances...
September 22, 2017: Journal of Clinical Laboratory Analysis
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