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Journal of Clinical Laboratory Analysis

Hyeon-Ho Lim, In-Hwa Jeong, Gyu-Dae An, Kwang-Sook Woo, Kyeong-Hee Kim, Jeong-Man Kim, Jae-Kwan Cha, Jin-Yeong Han
BACKGROUND: It is still not easy to predict severity promptly in patients with acute ischemic stroke (AIS) and transient ischemic attack (TIA). We investigated that platelet parameters or combinations of them could be a useful tool for early prediction of severity of AIS and TIA at admission and after 3 months. METHODS: We prospectively recruited 104 patients newly diagnosed with AIS and TIA. We investigated their neutrophil-to-lymphocyte ratio (NLR) and platelet parameters...
November 9, 2018: Journal of Clinical Laboratory Analysis
Guodong Xu, Changyi Wang, Xiuru Ying, Fanqian Kong, Huihui Ji, Jinshun Zhao, Xiaohong Zhang, Shiwei Duan, Liyuan Han, Li Li
BACKGROUND: Serine hydroxymethyltransferase 1 (SHMT1) is an enzyme involved in folic acid metabolism and is known to contribute to the development of hypertension. We evaluated the relationship between SHMT1 promoter methylation and essential hypertension (EH). METHODS: Quantitative methylation-specific polymerase chain reaction was used to measure the SHMT1 promoter methylation level in 241 EH patients and 288 age- and gender-matched healthy individuals. The diagnostic value of SHMT1 promoter hypermethylation was analyzed using a receiver operating characteristic (ROC) curve...
November 9, 2018: Journal of Clinical Laboratory Analysis
Çağlar Alp, Mehmet Tolga Dogru, Muhammed Karadeniz, Taner Sarak, Vahit Demir, Yunus Çelik, Hüseyin Kandemir
BACKGROUND: Endothelial dysfunction is one of the main pathological processes of hypertension. The association of serum pentraxin-3 (PTX3) levels and endothelial dysfunction becomes a more interesting scientific research issue due to high potential of PTX3 as a diagnostic and prognostic biomarker. We aimed to investigate the relationship between serum PTX3 levels and flow-mediated dilation results in patients with dipper and non-dipper hypertension. METHODS: This study included 90 hypertensive patients were divided into two groups based on 24 hours ambulatory blood pressure monitoring (ABPM): 38 patients with a dipper pattern and 52 patients with non-dipper pattern...
November 9, 2018: Journal of Clinical Laboratory Analysis
Yeniley Ruiz-Noa, Jorge Hernández-Bello, Mara A Llamas-Covarrubias, Claudia A Palafox-Sánchez, Edith Oregon-Romero, Pedro Ernesto Sánchez-Hernández, Maria Guadalupe Ramírez-Dueñas, Isela Parra-Rojas, Jose Francisco Muñoz-Valle
BACKGROUND: CD40 is a costimulatory molecule for B cells, and CD154 is a marker of CD4+ T cells activation. CD40-CD154 interaction promotes pro-inflammatory cytokines secretion and autoantibodies production. PTPN22 gene encodes LYP protein, an inhibitor of T- and B-cell activation. PTPN22 1858C>T polymorphism confers rheumatoid arthritis (RA) susceptibility. Hence, we evaluate the relationship between 1858C>T polymorphism with CD40 and CD154 expression and IFN-γ secretion in RA patients...
November 6, 2018: Journal of Clinical Laboratory Analysis
Hongmei Zhang, Yixin Niu, Hongxia Gu, Shuai Lu, Weiwei Zhang, Xiaoyong Li, Zhen Yang, Li Qin, Qing Su
BACKGROUND: The association between adiponectin and nonalcoholic fatty liver disease (NAFLD) has been studied before, but most of the studies are cross-sectional and cannot prove a causal link. OBJECTIVE: To prospectively investigate the relationship between serum adiponectin levels and the incidence of NAFLD in 3 years. SUBJECTS AND METHODS: A total of 1325 subjects aged 40 to 70 from the Chongming District of Shanghai, China, were included...
November 2, 2018: Journal of Clinical Laboratory Analysis
Morgane Choquet, Raphaël Guiheneuf, Sandrine Castelain, Emilie Pluquet, Véronique Decroix
BACKGROUND: Carbapenemase-producing Enterobacteriaceae (CPE) have become a major public health issue. The objective of the present study was to prospectively assess the analytical performance of a CPE detection algorithm based on phenotypic tests (the screening test) and MALDI-ToF hydrolysis (the confirmatory test). METHODS: Over a 6-month period and based on a disk diffusion method, 74 carbapenem-resistant strains were included in this study. RESULTS: Of the collected isolates, 54 turned out to be negative after phenotypic tests...
November 2, 2018: Journal of Clinical Laboratory Analysis
Linhong Liao, Hui Cheng, Fansheng Zeng, Weijie Zhou, Yanqing Ding
BACKGROUND: Human papillomavirus (HPV) infection can lead to the development of cervical cancer. This study assessed the genotype distribution of HPV of high-grade squamous intraepithelial lesion (HSIL) and invasive cervical cancer (ICC) in Ganzhou population. METHODS: A total of 935 females who got HPV testing from January 2016 to July 2018 in the maternal and child health hospital of Ganzhou were enrolled in the study, including 806 HSIL and 129 ICC. HPV detection and genotyping were tested by HPV Geno-Array test kit...
November 2, 2018: Journal of Clinical Laboratory Analysis
Muhammed Toprak, Omer Ates, Asker Zeki Ozsoy, Nihan Bozkurt, Saime Sezer Sondas, Bülent Cakmak, Hatice Yılmaz Dogru, İlhan Bahri Delibas, Fazlı Demirturk
BACKGROUND: Leiomyoma, one of the most common benign tumors, causes morbidity during the reproductive years in women. The molecular pathogenesis of the disease is not clear. Leiomyomas are hormone-sensitive tumors affecting around 20%-25% of women. Gene polymorphism studies could be important and explaining in the evaluation of multifactorial diseases such as leiomyoma. Polymorphisms involving genes responsible for the synthesis and signalization of steroid hormones could be used as genetic markers for hormone-related conditions...
November 2, 2018: Journal of Clinical Laboratory Analysis
Zuojian Hu, Huaping Chen, Li Huang, Siyuan Chen, Zhili Huang, Shanzi Qin, Jianing Zhong, Xue Qin, Shan Li
OBJECTIVE: Our aim intended to determine the relationship between hematological parameters (neutrophil-to-lymphocyte ratio [NLR], platelet-to-lymphocyte ratio [PLR], and eosinophil-to-lymphocyte ratio [ELR]) and ancylostomiasis. METHODS: There were 140 patients with ancylostomiasis and 159 healthy controls enrolled in this study. All data were collected from electronic medical records of the First Affiliated Hospital of Guangxi Medical University. RESULTS: The levels of NLR, PLR, and ELR in ancylostomiasis patients were significantly higher than those in the healthy controls (all P = 0...
November 2, 2018: Journal of Clinical Laboratory Analysis
Hee Jae Huh, Chang-Seok Ki, Sun Ae Yun, Jungsoo Lee, Gwi Young Oh, Nam-Sihk Lee, Young Ho Yoon, Nam Yong Lee
BACKGROUND: The DiaPlexQ™ STI6 Detection Kit (DiaPlexQ; Solgent Co., Ltd., Daejeon, South Korea) is a multiplex real-time PCR assay for the detection of the following sexually transmitted disease (STD) pathogens: Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis, Trichomonas vaginalis, Ureaplasma urealyticum, and Mycoplasma genitalium. We compared the performance of the DiaPlexQ assay with the GeneFinder™ STD I (CT/NG/UU) and STD II (MG/MH/TV) Multiplex Real-time PCR Kits (GeneFinder; Infopia Co...
November 2, 2018: Journal of Clinical Laboratory Analysis
Yong Pan, Wenjing Bai, Jiangnan Chen, Yijian Mao, Xiaohong Qian, Ke Xu, Shaohua Tang, Junwu Zhang, Chong Chen, Jingyi Chen, Xingzhong Hu
BACKGROUND: Malignant pleural effusion (MPE) is common and diagnosis is often problematic. A cancer ratio (serum lactate dehydrogenases: pleural adenosine deaminase ratio) has been proposed for diagnosing MPE. However, the usefulness of this "cancer ratio" and the clinical-radiological criteria for diagnosing MPE has not been clearly determined to date. The aim of this study was to assess the performance of those parameters in the diagnosis of MPE. METHODS: We analyzed 240 patients including 120 with MPE and 120 with non-MPE (93 tuberculous and 27 parapneumonic)...
November 2, 2018: Journal of Clinical Laboratory Analysis
Dongfeng Geng, Xiao Yang, Ruixue Wang, Shu Deng, Leilei Li, Xiaonan Hu, Yuting Jiang, Ruizhi Liu
BACKGROUND: Nonobstructive azoospermia (NOA) is one of the most severe forms of male infertility because of impaired spermatogenesis with the absence of spermatozoa in the ejaculate. The causes of this disease can be partly attributed to genetic factors. Some common structural variants and single nucleotide polymorphisms (SNPs) were reported to be associated with NOA. However, the underlying etiology and genetic mechanism(s) remain largely unclear. The aim of this study was to investigate the associated mutations of spermatogenic genes in Chinese infertile men with NOA...
November 2, 2018: Journal of Clinical Laboratory Analysis
Nikolaos Grivas, Daan de Bruin, Kurdo Barwari, Erik van Muilekom, Corinne Tillier, Pim J van Leeuwen, Esther Wit, Wouter Kroese, Henk van der Poel
BACKGROUND: Ultrasensitive prostate-specific antigen (USPSA) is useful for stratifying patients according to their USPSA-based risk. Aim of our study was to determine the usefulness of USPSA as predictor of biochemical recurrence (BCR) after robot-assisted radical prostatectomy (RARP). METHODS: This retrospective study included 213 prostate cancer patients who had a postoperative USPSA between 0.01 and 0.2 ng/mL and at least 2 years of follow-up. We developed predictive models for BCR with PSA ≥0...
October 26, 2018: Journal of Clinical Laboratory Analysis
Andrea Ceri, Marina Pavic, Ivana Horvat, Margareta Radic Antolic, Renata Zadro
BACKGROUND: High resolution melting (HRM) analysis is one of the newer, reliable, and sensitive genotyping techniques, which offers considerable time and cost savings. P-selectin is an adhesion molecule that has a role in the initial phases of leukocyte adhesion to stimulated platelets and endothelial cells in inflammation. Multiple polymorphisms in P-selectin gene (SELP) that affect the protein sequence have been described. The aim of this study was to design, optimize, and validate a simple and rapid in-house HRM-based method for genotyping the NM_003005...
October 23, 2018: Journal of Clinical Laboratory Analysis
Shengliang Zhou, Shinian Cao, Guoliang Ma, Tielin Ding, Jingjing Mu, Weilu Han, Dongxu Sun, Caifa Chen
BACKGROUND: Early diagnosis of hepatitis C virus (HCV) infection is very important for the treatment of the disease. Development of sensitive and specific rapid detection assays is of great significance for the diagnosis. Here, we describe a promising method of using gold-labeled streptavidin fusion proteins as novel signal reporter in a rapid detection assay for HCV infection. METHODS: Recombinant genes encoding streptavidin fused with Escherichia coli maltose-binding protein (MBP) or with a portion of bacterial translational initiation factor 2 were cloned in expression vectors pMAL-5CX and pET28 and transformed in proper Escherichia coli host strains...
October 23, 2018: Journal of Clinical Laboratory Analysis
Yabo Yang, Miao Ding, Na Di, Ricardo Azziz, Dongzi Yang, Xiaomiao Zhao
BACKGROUND: To investigate the correlation between hyperandrogenism (HA) and insulin resistance (IR) in women with polycystic ovary syndrome (PCOS) by measuring serum total testosterone (TT) using a liquid chromatography and tandem mass spectrometry assay (LC-MS/MS). METHODS: This cohort study included 332 patients with PCOS, 63 patients with IR and 276 with controls. TT levels were measured by LC-MS/MS and chemiluminescent immunoassay (CLIA); glucose and insulin levels were determined by an oral glucose tolerance test (OGTT)...
October 23, 2018: Journal of Clinical Laboratory Analysis
Ping-Ping Dong
OBJECTIVE: To study the associations of vascular endothelial growth factor (VEGF) expression and its gene polymorphisms with the risk of gestational diabetes mellitus (GDM). METHODS: A total of 239 GDM patients (GDM group) and 275 healthy pregnant women (Control group) were included in this study. VEGF genotypes (including rs2146323, rs2010963, rs3025039, rs3025010, and rs833069) were analyzed by TaqMan assay. ELISA was used to determine the serum VEGF levels. The software SHEsis was performed to analyze haplotypes...
October 23, 2018: Journal of Clinical Laboratory Analysis
Luz E Chavarria-Buenrostro, Jorge Hernandez-Bello, Jose F Muñoz-Valle, Jose Macias-Barragan, Liliana B Hernandez-Carrillo, Jorge F Topete-Reyes, Renato Parra-Michel, Maria G Ramirez-Dueñas, Pedro E Sanchez-Hernandez, Ana L Pereira-Suarez, Eden Oceguera-Contreras, Margarita Montoya-Buelna
INTRODUCTION: Diabetic Nephropathy (DN) is the main cause of chronic kidney disease (CKD) in diabetic patients. An IL-10 imbalance could be related to renal hypertrophy and trigger to nephropathy. Three promoter polymorphisms (-1082G>A, -819C>T, and -592C>A) at IL10 gene have been associated with changes in the IL-10 expression and DN susceptibility. Therefore, the aim of this study was to analyze this association in Mexican patients with DN. METHODS: We conducted a case-control study on 128 patients with DN and 150 control subjects (CS) from western Mexico...
October 21, 2018: Journal of Clinical Laboratory Analysis
Tingting Niu, Yang Liu, Feifei Zhu, Jiayan Ma, Jimin Gao
BACKGROUND: Serological tests are indispensable in the diagnosis of early infection. At present, only procalcitonin (PCT) and C-reactive protein (CRP) are commonly used in clinical practice. Recently, serum amyloid A1 (SAA1) and heparin binding protein (HBP) have been shown to be new biomarkers, because SAA1 is highly sensitive and specific for viral infections, and HBP is predictive for septic shock. In this study, PCT, CRP, HBP, and SAA1 were detected in different combinations to improve the diagnostic accuracy of early infection using the biotin-avidin amplifying system-based time-resolved fluorescent immunoassay (BA-TRFIA)...
October 18, 2018: Journal of Clinical Laboratory Analysis
Masafumi Koga, Shinya Inada, Hiroko Ijima, Hideaki Jinnouchi, Yasuhiro Ono, Tsuyoshi Iwasaka, Toshika Okumiya
BACKGROUND: The hyperglycemic state is known to shorten the erythrocyte life span. Erythrocyte creatine (EC) reflects the mean erythrocyte age and is useful as an indicator of hemolysis. Here, we studied the relationship between EC and glycemic control indicators [HbA1c or glycated albumin (GA)] in non-diabetic subjects and diabetic patients. METHODS: This study included 119 patients with type 2 diabetes mellitus (T2DM) and 76 non-diabetic subjects matched by sex and age...
October 18, 2018: Journal of Clinical Laboratory Analysis
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