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Journal of Clinical Laboratory Analysis

Fengxia Zhan, Shuzhen Zhu, Haiying Liu, Qian Wang, Guanghui Zhao
OBJECTIVE: To assess the value of blood routine test (blood RT) in order to predict the occurrence of premature rupture of membranes (PROM). METHODS: A retrospective study was conducted to collect blood RT data from 100 cases of preterm premature rupture of membranes (pPROM), 70 cases of full-term premature rupture of membranes (fPROM), and 100 cases of full-term pregnancy (Normal). Nonparametric tests were performed for each blood routine parameter, the ROC curve was established for the parameters with significant difference, and the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), positive likelihood ratios (+LR), and negative likelihood ratios (-LR) were further calculated...
September 21, 2018: Journal of Clinical Laboratory Analysis
Junhua Cao, Lijun Tian, Zhenguang Li, Chonglin Zhang, Qiang Ji, Chuanling Zhang, Tong Qian
BACKGROUND: Recently studies uncovered associations between polymorphisms of interleukin genes and the risk of asthma. However, the relationship between polymorphisms of interleukin-7 gene and the risk of children asthma has not been discovered yet. This study aims to investigate the relationship between single nucleotide polymorphisms (SNPs) on interleukin-7 gene and the risk of children asthma. METHODS: We genotyped eight SNPs of interleukin-7 gene in blood samples from 437 asthma patients and 489 healthy controls to analyze potential associations of these SNPs with the risk of asthma in children...
September 21, 2018: Journal of Clinical Laboratory Analysis
Shaoyou Qin, Song Wang, Xu Wang, Jiangbin Wang
BACKGROUND: This study was designed to determine the diagnostic value of liver stiffness measured by transient elastography (TE) in identifying chronic kidney disease (CKD) in individuals with ultrasonography-diagnosed NAFLD. METHODS: A total of 1439 adult patients with ultrasonography-diagnosed NAFLD between October 2015 and August 2017 in China-Japan union hospital of Jilin university were initially eligible. According to the exclusion criteria, 24 patients were excluded, and eventually, a total of 1415 patients were included in the study...
September 21, 2018: Journal of Clinical Laboratory Analysis
Bin Wang, Yusheng Xue, Fujun Shang, Sifeng Ni, Xiongtao Liu, Boyuan Fan, Haichang Wang
OBJECTIVE: Adropin, a newly identified regulatory protein encoded by Enho gene, suppressed tumor necrosis factor α-induced THP1 monocyte adhesion to human umbilical vein endothelial cells. In addition, inflammation is demonstrated to be involved in the mechanism of atrial fibrillation (AF). Atrial remodeling is correlated with the persistence and progression of AF. Adropin is hypothesized to correlated with AF and atrial remodeling. This study aims to determine the correlation of serum adropin and the presence of AF and remodeling...
September 21, 2018: Journal of Clinical Laboratory Analysis
Jianli Ge, Shasha Geng, Hua Jiang
BACKGROUND: We aimed to assess the predictive value of long noncoding RNAs antisense noncoding RNA in the INK4 locus (lncRNAs ANRIL) for acute exacerbation of chronic obstructive pulmonary disease (COPD) and evaluate its correlation with inflammatory cytokines as well as the Global Initiative for Chronic Obstructive Lung Disease (GOLD) stage in COPD patients. METHODS: A total of 136 acute exacerbations of COPD (AECOPD) patients, 138 stable COPD patients, and 140 healthy controls (HCs) were consecutively recruited, and plasma samples were collected...
September 19, 2018: Journal of Clinical Laboratory Analysis
Yanqiang Wu, Xianghua Fu
BACKGROUND: This study aimed to explore the correlation of baseline, procedural, and postprocedure characteristics with the risk of rapid angiographic stenotic progression (RASP) and restenosis in coronary artery disease (CAD) patients after percutaneous coronary intervention (PCI) with drug-eluting stents implantation. METHODS: Two hundred fourteen CAD patients underwent PCI with drug-eluting stents implantation were consecutively enrolled. Baseline, procedural, and postprocedure characteristics of patients were collected for analysis...
September 17, 2018: Journal of Clinical Laboratory Analysis
Chun-Hai Gao, Bao-Ju Ji, Chao Han, Mao-Shui Wang
BACKGROUND: The study aimed to compare enzyme-linked immunosorbent assay (ELISA) with indirect immunofluorescence assay (IFA) in the diagnosis of Mycoplasma pneumoniae infection. METHODS: From March 2016 to May 2017, 180 patients suspected with M. pneumoniae infection were enrolled. The SeroMP kit using ELISA and PNEUMOSLIDE kit using IFA were performed in parallel to detect the IgM antibodies against M. pneumoniae. Cohen's kappa statistics were used to assess the agreement between the ELISA and IFA assays, multivariate logistic regression analysis was used to evaluate risk factors for the discordance between the ELISA and IFA assays...
September 17, 2018: Journal of Clinical Laboratory Analysis
Saqib Ansari, Nabil Rashid, Anny Hanifa, Saima Siddiqui, Bushra Kaleem, Arshi Naz, Kousar Perveen, Zeeshan Hussain, Iqra Ansari, Qammar Jabbar, Tahir Khan, Muhammad Nadeem, Tahir Shamsi
BACKGROUND: Differentiation between thalassemia major and thalassemia intermedia at presentation is not uniformly characterized, for which an absolute criteria needs to be developed. This study investigated the primary and secondary genetic modifiers to develop a laboratory finding by forming different genetic mutational combinations seen among thalassemia intermedia patients and comparing them with thalassemia major. METHODS: This cross-sectional study analyzed 315 thalassemia intermedia patients...
September 17, 2018: Journal of Clinical Laboratory Analysis
Tsachi Tsadok Perets, Rachel Gingold-Belfer, Haim Leibovitzh, David Itskoviz, Hemda Schmilovitz-Weiss, Yifat Snir, Ram Dickman, Iris Dotan, Zohar Levi, Doron Boltin
BACKGROUND: Threshold values for 13 C-urea breath test (13C-UBT) positivity may be affected by various sociodemographic, host, bacterial, and laboratory factors. Manufacturer recommended cutoffs for 13C-UBT assays may not be applicable in all settings. Optimizing 13C-UBT cutoffs may have profound public health ramifications. We aimed to determine the optimal threshold for 13C-UBT positivity in our population. METHODS: Consecutive test samples collected at our central laboratory from patients undergoing a first-time 13C-UBT between 1 January 2010 and 31 December 2015 were included...
September 17, 2018: Journal of Clinical Laboratory Analysis
Amitava Dasgupta, Laverne Bourgeois
BACKGROUND: Although biotin interferences in TSH, FT3, FT4, and other biotinylated antibody-based assays manufactured by Roche Diagnostics have been well studied, there are relatively few reports on biotin interference in biotin-based assays manufactured by other companies. We investigated biotin interferences in TSH, FT4, and FT3 assays based on the LOCI (luminescent oxygen channeling assay) technology using the Dimension Vista 1500 analyzer (Siemens). METHODS: We prepared four serum pools using leftover specimens...
September 17, 2018: Journal of Clinical Laboratory Analysis
Zhetao Li, Jiwei Huang, Biao Liang, Dingyuan Zeng, Shiqiang Luo, Tizhen Yan, Fengwen Liao, Jun Huang, Jingwen Li, Ren Cai, Xine Deng, Ning Tang
BACKGROUND: Congenital heart disease (CHD) is a common birth defect originating from both environmental and genetic factors. An overabundance of copy number variations (CNVs) affecting cardiac-related genes has previously been detected in individuals with CHD. OBJECTIVE: To evaluate if the presence of CNVs in the 22q11.2 region, and to determine whether GATA4, NKX2-5, TBX5, BMP, and CRELD1 genes contributed toward the pathogenesis of isolated incidences of CHDs in southwest China...
September 17, 2018: Journal of Clinical Laboratory Analysis
Long Li, Yulan Qin, Yajie Su, Haili Jiang, Nuerya Rejiafu, Mingzhu Li, Ayijiamali Muhetaer, Yongqiao Liu, Yan Ren
BACKGROUND: Tetrahydrobiopterin (BH4 ) deficiency is an autosomal recessive disorder, which is caused by an enzyme deficiency involved in its synthetic or metabolic pathways. Clinical symptoms may include microcephaly, hypoevolutism, severe ataxia, and seizures. The purposes of this study are to analyze the genotype-phenotype and the pedigree of the first case of BH4 deficiency in the Uygur of China. METHODS: (a) This patient received tandem mass spectrometry, urinary neopterin and biopterin analysis, and determination of dihydropteridine reductase (DHPR) activity in dried blood spots...
September 17, 2018: Journal of Clinical Laboratory Analysis
Anping Xu, Jian Sun, Jie Li, Weidong Chen, Ruoyang Zheng, Zhonghou Han, Ling Ji
BACKGROUND: HbA1c is the standard bio-marker for glycemic control in patients with diabetes. Here, we report a α-globin chain variant and evaluate its effect on HbA1c measurements. METHODS: A 21-year-old female was suspected of harboring a hemoglobin variant following HbA1c measurement during a routine examination using Variant II Turbo 2.0 (Bio-Rad). An oral glucose tolerance test was performed using an AU5800 clinical chemistry system (Beckman Coulter). HbA1c was reanalyzed using D10 (Bio-Rad), Capillarys 2 Flex Piercing (Sebia), and Premier Hb9210 (Trinity Biotech)...
September 17, 2018: Journal of Clinical Laboratory Analysis
Haihong Xu, Xueying Zhang, Wenwu Yao, Yi Sun, Yanjun Zhang
BACKGROUND: Cytolytic vaginosis (CV) is a common disease that results in pruritus, dyspareunia, and vulvar dysuria. However, the pathological mechanisms of the disease are still unclear. Compared to traditional methods, high-throughput sequencing can obtain more accurate qualitative and quantitative information on the microbiome. METHODS: We collected 75 samples from 32 healthy women (average age 44 ± 8) and 43 patients with CV (average age 38 ± 8). We used high-throughput sequencing of the 16S rRNA V3-V4 region to characterize and compare the vaginal microbiota of patients with CV and healthy women and to identify potential biomarkers for CV...
September 10, 2018: Journal of Clinical Laboratory Analysis
Yunlong Zhao, Yingjie Li, Ping Chen, Shaojun Li, Jian Luo, Hui Xia
BACKGROUND: The ratio of target cfDNA in total plasma is low. The abundant gDNA background resulting from blood cell lysis caused by improper operation has become a major obstacle to accurately measure cfDNA. In this study, we investigated the storage capacity of three blood cell collection tubes (BCTs) in the prevention of genomic DNA contamination caused by white blood cell rupture and evaluated their performance when they were utilized combining with highly sensitive mutation detection technology...
September 7, 2018: Journal of Clinical Laboratory Analysis
Hassan Ghasemi, Jamshid Karimi, Iraj Khodadadi, Massoud Saidijam, Heidar Tavilani
BACKGROUND: Angiopoietin-like protein 8 (ANGPTL8) is a hormone that mainly secreted from the liver and adipose tissue and plays an important role in the proliferation of pancreatic beta cells and lipid metabolism. Therefore, we studied the association of ANGPTL8 rs2278426 (C/T) and rs892066 (C/G) polymorphisms with the risk of type 2 diabetes mellitus (T2DM) and their association with biochemical parameters. METHODS: Two hundred and eighty-eight subjects (controls; n = 138 and type 2 diabetic patients; n = 150) were enrolled in this study...
September 7, 2018: Journal of Clinical Laboratory Analysis
Pei Li, Jun Ning, Xipeng Luo, Hongli Du, Qing Zhang, Ganlin Zhou, Qiu Du, Zhenyu Ou, Long Wang, Yu Wang
BACKGROUND: Due to high nuclease activity and complex contents in urine, urinary cell-free DNA (ucfDNA) was prone to degrade. So, we developed standardized urine collection tube (UCT) to prevent ucfDNA degradation and simultaneously maintain urinary cells in their original form during the sample collection process, ensuring stabilization of the original proportion and integrity of ucfDNA. METHODS: Urine samples were collected from bladder cancer patients and divided into 10-mL normal tubes and 10-mL UCTs, respectively, and kept at ambient temperature...
September 2, 2018: Journal of Clinical Laboratory Analysis
Nilüfer Okur, Cüneyt Tayman, Mehmet Büyüktiryaki, Gülsüm Kadıoğlu Şimşek, Buse Ozer Bekmez, Nahide Altuğ
OBJECTIVE: Serum lactate levels provide information on metabolic capacity at the cellular level. In addition, lactate reflects tissue perfusion and oxygenation status. The aim of this study was to determine the usefulness of high lactate levels as a marker in hemodynamically significant patent ductus arteriosus (hsPDA), which may lead to tissue perfusion defects. METHODS: Preterm infants with gestational age ≤32 weeks and birthweight ≤1500 g were included...
September 2, 2018: Journal of Clinical Laboratory Analysis
Minh Hoang, Hung-Yi Wu, Ying-Xiu Lien, Ming-Tang Chiou, Chao-Nan Lin
BACKGROUND: Canine parvovirus type 2 (CPV-2) causes an important canine viral disease worldwide. CPV-2 belongs to the Protoparvovirus genus in the family Parvoviridae. An amino acid change at position 426 of the VP2 protein differentiate types of CPV-2, designated as CPV-2a (Asn), CPV-2b (Asp), and CPV-2c (Glu). In this study, we compared CPV-2 genotyping results obtained by SimpleProbe® real-time PCR and DNA sequencing analysis to identify the accuracy and sensitivity of these methods...
August 31, 2018: Journal of Clinical Laboratory Analysis
Changqiang Chen, Peizhan Chen, Xuqian Fang
BACKGROUND: Monoclonal free light chains (FLC) commonly exist in monomeric or dimeric forms but rarely as larger molecules. Little is known about whether polymeric molecules can affect urine protein electrophoresis (UPE) results. METHODS: Urine samples were collected from 72 multiple myeloma (MM) patients with Bence Jones protein (BJP). Urine protein and immunofixation electrophoresis were analyzed on Sebia SDS "agarose" gel electrophoresis system (SDS-AGE), and immunoglobulin free light chains were measured on the BNII nephelometric assay...
August 27, 2018: Journal of Clinical Laboratory Analysis
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