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Immunopharmacology and Immunotoxicology

Wei Wu, Yinfang Li, Zelin Jiao, Li Zhang, Xiaohua Wang, Rui Qin
BACKGROUND: Asthma is a chronic airway immunoinflammatory disorder characterized by airway remodeling. Phyllanthus amarus has been reported to possess antioxidant and anti-inflammatory potential. AIM: To evaluate the possible mechanism of action of isolated phytoconstituents from P. amarus (PA) against ovalbumin (OVA)-induced experimental airway hyperresponsiveness (AHR). MATERIAL AND METHOD: Phyllanthin and hypophyllanthin were isolated and characterized (HPLC) from the methanolic extract of PA...
December 12, 2018: Immunopharmacology and Immunotoxicology
Moisés Armides Franco-Molina, Yareellys Ramos-Zayas, Erika Evangelina Coronado-Cerda, Edgar Mendoza-Gamboa, Pablo Zapata-Benavides, Silvia Elena Santana-Krymskaya, Reyes Tamez-Guerra, Cristina Rodríguez-Padilla
OBJECTIVE: The aim of the present study was to evaluate the therapeutic potential of autologous DCs loaded with whole tumor cell lysate of CTVT generated under a simplified and rapid procedure in vitro production process, in a vulvar submucosal model of CTVT in dogs. MATERIALS AND METHODS: We generated a model of intravulvar CTVT in dogs. A CTVT lysate antigen was prepared according to the method of 1-butanol and after administered with complete Freund's adjuvant via subcutaneous in female healthy dogs and challenge with CTVT cells to corroborate the immunogenicity...
December 3, 2018: Immunopharmacology and Immunotoxicology
Ali-Mohammad Rousta, Seyed-Mohamad-Sadegh Mirahmadi, Alireza Shahmohammadi, Davood Nourabadi, Mohammad-Reza Khajevand-Khazaei, Tourandokht Baluchnejadmojarad, Mehrdad Roghani
CONTEXT: Acute kidney injury (AKI) is considered a major public health concern in today's world. Sepsis-induced AKI is large as a result of exposure to lipopolysaccharide (LPS) that is the major outer membrane component of Gram-negative bacteria. Sesamin is the main lignan of sesame seeds with multiple protective effects. OBJECTIVE: In this research, we tried to demonstrate the protective effect of sesamin pretreatment in LPS-induced mouse model of AKI. METHODS: LPS was injected at a single dose of 10 mg/kg (i...
November 29, 2018: Immunopharmacology and Immunotoxicology
De-Zhi Zheng, Yan-Min Bu, Lei Wang
OBJECTIVE: To reveal other miR-130b-mediated signaling pathway in the involvement of wear particle-induced inflammation and osteolysis. MATERIALS AND METHODS: Particle-induced osteolysis (PIO) mice model was established. Secretion levels of TNF-α, IL-1β, IL-6, and IL-10 were measured by ELISA. miR-130b and forkhead box F2 (FOXF2) mRNA were detected by qRT-PCR. Protein levels of FOXF2, phosphorylation-p65 (p-p65), and p-IκB were observed by Western blot. Luciferase reporter assay was performed to confirm the regulation of miR-130b on FOXF2...
November 29, 2018: Immunopharmacology and Immunotoxicology
Amir Nejad-Moghaddam, Eisa Tahmasbpour, Milad Sohrabiyan, Hosein Jafari, Mostafa Ghanei
Sulfur mustard (SM) is a toxic agent which causes severe abnormalities in an airway system such as necrosis and inflammation, oxidative stress, chronic bronchitis, shortness of breath, and chronic obstructive pulmonary disease. Although possible mechanisms of SM toxicity have been extensively considered, there is still need to find an appropriate clinical treatment to decrease chronic lung injuries caused by SM. Due to extensive progresses and achievement in tissue repairing through stem cells therapy, the importance of cell therapy for the treatment of lung injuries has been increased...
November 29, 2018: Immunopharmacology and Immunotoxicology
Jaewoong Jang, Yoonju Jung, Seyeon Chae, Taehyun Bae, Seok-Min Kim, Yae Jie Shim, Sang-In Chung, Yoosik Yoon
AIM: In this study, we report the anti-inflammatory activity of XAV939, a modulator of the Wnt/β-catenin pathway. METHODS: WNT/β-catenin pathway and NF-κB signaling pathway were examined in LPS-stimulated human bronchial epithelial cells and effects of XAV939 on these pathways were analyzed. The effect of XAV939 was confirmed in human umbilical vein endothelial cells. RESULTS: LPS-induced expressions of pro-inflammatory genes IL-6, IL-8, TNF-α, IL-1β, MCP-1, MMP-9, iNOS and COX-2 were significantly and dose-dependently suppressed by XAV939...
November 22, 2018: Immunopharmacology and Immunotoxicology
Sunil Gowda S N, Raghavi Raviraj, Devipriya Nagarajan, Weiling Zhao
Lung cancer continues to be the leading cause of cancer deaths and more than one million lung cancer patients will die every year worldwide. Radiotherapy (RT) plays an important role in lung cancer treatment, but the side effects of RT are pneumonitis and pulmonary fibrosis. RT-induced lung injury causes damage to alveolar-epithelial cells and vascular endothelial cells. Macrophages play an important role in the development of pulmonary fibrosis despite its role in immune response. These injury activated macrophages develop into classically activated M1 macrophage or alternative activated M2 macrophage...
November 16, 2018: Immunopharmacology and Immunotoxicology
Nuo Xu, Wenjuan Deng, Gaiying He, Xiaoshuang Gan, Shuang Gao, Yu Chen, Yitian Gao, Ke Xu, Junmei Qi, Haojie Lin, Li Shen, Xiaokun Li, Zhenlin Hu
OBJECTIVE: Acne is a chronic skin disease that involves four key pathogenic factors: excess sebum production, ductal epidermal hyperproliferation, Propionibacterium acnes (P. acnes) colonization, and skin inflammation. Mangostins are well-known for their anti-bacterial and anti-inflammatory effects, suggesting that mangostins may have therapeutic potential for acne. The present study aimed to explore the anti-acne effects of mangostins from the perspective of multiple pathogenic mechanisms of acne...
November 13, 2018: Immunopharmacology and Immunotoxicology
Dina A Hanna, Marwa M Khalaf, Ali A Abo-Saif
CONTEXT: Prevalence of bronchial asthma massively increases worldwide, while the frequent therapies are still not sufficient. Polydatin, a naturally occurring glycoside, was known as to have anti-inflammatory and anti-oxidant effects. OBJECTIVE: The current study aimed to investigate the possible protective effect of polydatin against experimental bronchial asthma in rats. MATERIAL AND METHODS: Bronchial asthma was induced by ovalbumin (OVA) sensitization and challenge...
November 13, 2018: Immunopharmacology and Immunotoxicology
Zhen Xiang, Wei Chen, Jun Zhang, Shuzheng Song, Guang-Kai Xia, Xin-Yu Huang, Juan Xie, Yingyan Yu, Qing-Yuan Zhang
OBJECTIVE: Recently, immune checkpoints blockers showed higher anti-tumor activity for advanced gastric cancer (GC). The purpose of the study is to find out predictive biomarkers related to anti-cytotoxic lymphocyte antigen 4 (CTLA4) therapy. MATERIALS AND METHODS: Datasets of gene expression omnibus (GEO), the cancer genome atlas (TCGA), and gene set enrichment analysis (GESA) were extracted. Differential expression of CTLA4 between cancer tissues and normal mucosa, enrichment of WT (wild type) vs...
November 13, 2018: Immunopharmacology and Immunotoxicology
Weixiong Guo, Bo Wei, Jiecong Sun, Tingting Chen, Jinsong Wei, Zibing Hu, Siyuan Chen, Min Xiang, Yang Shu, Zhiheng Peng
OBJECTIVE: To explore the effect of suppressive oligodeoxynucleotide-induced dendritic cells (S-DCs) in the osteoarthritis (OA) therapy. METHODS: S-DCs were prepared from splenic CD11c + cells by in vitro culture with suppressive oligodeoxynucleotide. The function and phenotypes of S-DCs were measured by ELISA and flow cytometry. The innate immune signaling pathways were detected by western blotting in the non-treated DCs and S-DCs upon stimulation. In vivo, we employed an iodoacetate-induced OA mice model...
October 26, 2018: Immunopharmacology and Immunotoxicology
Yareellys Ramos-Zayas, Moisés Armides Franco-Molina, Alex Jesús Hernádez-Granados, Diana Ginette Zárate-Triviño, Erika Evangelina Coronado-Cerda, Edgar Mendoza-Gamboa, Pablo Zapata-Benavides, Rafael Ramírez-Romero, Silvia Elena Santana-Krymskaya, Reyes Tamez-Guerra, Cristina Rodríguez-Padilla
Exosomes secreted by tumor cells are a good source of cellular components that stimulate the immune response, such as alarmins (mRNA, tetraspanins (CD9, CD63, CD81), heat-shock proteins, major histocompatibility complex class I molecules) and tumor-associated antigens. These properties permit to pulsed dendritic cells in the immunotherapy for many cancers types. The aim of this study was to demonstrate the use of exosomes derived from canine transmissible venereal tumor (CTVT) as an antigen to pulsed dendritic cells and its administration in dogs with CTVT as treatment against this disease...
October 18, 2018: Immunopharmacology and Immunotoxicology
Negar Vanaki, Taghi Golmohammadi, Ahmadreza Jamshidi, Maryam Akhtari, Mahdi Vojdanian, Shayan Mostafaei, Shiva Poursani, Nooshin Ahmadzadeh, Mahdi Mahmoudi
BACKGROUND: Ankylosing spondylitis (AS) is a common debilitating rheumatic disease in which the innate immune components especially the Interleukin (IL)-23/IL-17 axis related genes play important role in its pathogenesis. Nucleotide binding oligomerization domain-containing protein (NOD)2, as an innate receptor, is critical for IL-23 production in cells. Therefore, we aimed to stimulate NOD2 signaling and study its effects on cytokine production in peripheral blood mononuclear cells (PBMC) of these patients...
September 28, 2018: Immunopharmacology and Immunotoxicology
Saeed Daneshmandi, Ali Akbar Pourfathollah, Mehdi Forouzandeh-Moghaddam
OBJECTIVE: To improve dendritic cells (DCs) function, we targeted DCs to over express CD40 and inducible costimulator ligand (ICOSL) costimulatory molecules along with total messenger RNA (mRNA) of tumor cells to achieve a safe and effective system for treatment of tumor. MATERIALS AND METHODS: We generated CD40 and ICOSL mRNA in vitro and manipulated DCs using chitosan nanoparticles and also lipofectamine transfection system then examined in vitro and in vivo. RESULTS: Mice bone marrow derived DCs pulsed with total tumor mRNA/CD40 mRNA or ICOSL mRNA showed higher expression of DCs maturation markers (CD40, ICOSL, CD86, and MHC-II) and accelerated secretion of pro-inflammatory cytokines...
September 28, 2018: Immunopharmacology and Immunotoxicology
Na-Ra Han, Phil-Dong Moon, Hyung-Min Kim, Hyun-Ja Jeong
OBJECTIVES: Atopic dermatitis (AD) is an allergic and inflammatory skin disorder caused by a combination of itching and skin sensitization by allergens. This article investigated whether cordycepin modulates AD symptoms by using an AD murine model. MATERIAL AND METHODS: We evaluated a regulatory effect and specific molecular mechanism of cordycepin on AD induced by the repeated local exposure of 2,4-dinitrochlorobenzene to dorsal skin of mice. Blood or AD-like skin lesions samples were removed for histopathologic analysis, enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, and Western blot analyses...
September 5, 2018: Immunopharmacology and Immunotoxicology
Rasoul Baharlou, Nader Tajik, Mahdi Behdani, Mohammad Ali Shokrgozar, Vajiheh Tavana, Fatemeh Kazemi-Lomedasht, Fatemeh Faraji, Mahdi Habibi-Anbouhi
OBJECTIVES: Angiogenesis targeting is an attractive approach for cancer treatment. Delta-like ligand 4 (DLL4) plays a pivotal role in neovascular development and its inhibitors have recently entered clinical trials for solid tumors. The aim of this study was to evaluate the possibilities of using anti-DLL4 antibody fragment as an angiogenesis maturation inhibitor. MATERIALS AND METHODS: In this study, a DLL4-specific Nanobody, named 3Nb3, was selected and assessed by western blotting and internalization assays...
September 5, 2018: Immunopharmacology and Immunotoxicology
Ana Paula Fortes Dos Santos Thomazelli, Fernanda Tomiotto-Pellissier, Milena Menegazzo Miranda-Sapla, Suelen Santos da Silva, Daniele Sapede Alvarenga, Carolina Panis, Allan Henrique Depieri Cataneo, Juliano Bordignon, Guilherme Ferreira Silveira, Lucy Megumi Yamauchi, Jussevania Pereira Santos Rubro de Sá, Ionice Felipe, Wander Rogério Pavanelli, Ivete Conchon-Costa
The context of the article: Leishmania amazonensis has a wide geographical distribution throughout South American countries and can cause self-healing to severe cases as mucocutaneous or visceral forms. Leishmaniasis presents a balance of inflammatory and anti-inflammatory cytokines which is responsible for promoting the activation of phagocytes, essential to control the infection and lead to tissue repair/resolution of the disease, respectively. RESULTS AND DISCUSSION: Our model revealed that the treatment with Con-A was capable to stimulate human PBMC cells by increasing the phagocytic capacity and promoting parasite elimination...
September 5, 2018: Immunopharmacology and Immunotoxicology
Yiqing Wang, Qichun Tang, Peibei Duan, Lihua Yang
Ulcerative colitis (UC) is a chronic, relapsing, remitting, and inflammatory disorder that afflicts millions of people around the world. It carries a substantial economic burden, reducing the quality of life, ability to work, and increasing disability. Conventional medical treatment of UC includes the use of aminosalicylates, corticosteroids, and immunosuppressive drugs. However, these medicines are not always effective due to some serious side effects. Nuclear factor-kappa B (NF-κB) is a key factor in the inflammatory setting and strongly affects the course of mucosal inflammation in UC...
August 15, 2018: Immunopharmacology and Immunotoxicology
Fu-Sheng Gao, Yu-Tao Zhan, Xu-Dong Wang, Chuan Zhang
AIM: DNA vaccines have emerged as a promising strategy for cancer immunotherapy; however, their immunogenicity is weak. Fms-like tyrosine kinase 3-ligand (Flt3L) has been exploited for its ability to increase the proliferation of dendritic cells (DCs). The aim of the present study was to investigate whether co-administration of an adjuvant plasmid expressing mouse Flt3L and a DNA vaccine of the Mucin 1 (MUC1) antigen enhances immune responses. METHODS: The recombinant plasmids pVAX1-MUC1 and pVAX1-Flt3L were constructed and injected into mice intramuscularly (i...
August 2018: Immunopharmacology and Immunotoxicology
Dong Xu, Hongying Zhu, Qingdong Fu, Songxiao Xu, Wen Sun, Guoxiao Chen, Xiaoli Lv
OBJECTIVE: Lens epithelial cell (LEC) membrane damage is one of the pathogenesis of cataract. High mobility group box-1 (HMGB-1) and nuclear factor-κB (NF-κB) play vital roles in a variety of diseases, such as inflammation. Ketamine has numerous pharmacological effects that can inhibit inflammation. However, its role in cataract rats LECs has not yet been elucidated. MATERIALS AND METHODS: LECs were isolated from SD rats and cultured in vitro. The cells were randomly divided into three groups, including the control group, cataract model group induced by H2 O2 , and ketamine group treated by 10 mM ketamine under H2 O2 environment...
August 2018: Immunopharmacology and Immunotoxicology
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