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Immunopharmacology and Immunotoxicology

Negar Vanaki, Taghi Golmohammadi, Ahmadreza Jamshidi, Maryam Akhtari, Mahdi Vojdanian, Shayan Mostafaei, Shiva Poursani, Nooshin Ahmadzadeh, Mahdi Mahmoudi
BACKGROUND: Ankylosing spondylitis (AS) is a common debilitating rheumatic disease in which the innate immune components especially the Interleukin (IL)-23/IL-17 axis related genes play important role in its pathogenesis. Nucleotide binding oligomerization domain-containing protein (NOD)2, as an innate receptor, is critical for IL-23 production in cells. Therefore, we aimed to stimulate NOD2 signaling and study its effects on cytokine production in peripheral blood mononuclear cells (PBMC) of these patients...
September 28, 2018: Immunopharmacology and Immunotoxicology
Saeed Daneshmandi, Ali Akbar Pourfathollah, Mehdi Forouzandeh-Moghaddam
OBJECTIVE: To improve dendritic cells (DCs) function, we targeted DCs to over express CD40 and inducible costimulator ligand (ICOSL) costimulatory molecules along with total messenger RNA (mRNA) of tumor cells to achieve a safe and effective system for treatment of tumor. MATERIALS AND METHODS: We generated CD40 and ICOSL mRNA in vitro and manipulated DCs using chitosan nanoparticles and also lipofectamine transfection system then examined in vitro and in vivo. RESULTS: Mice bone marrow derived DCs pulsed with total tumor mRNA/CD40 mRNA or ICOSL mRNA showed higher expression of DCs maturation markers (CD40, ICOSL, CD86, and MHC-II) and accelerated secretion of pro-inflammatory cytokines...
September 28, 2018: Immunopharmacology and Immunotoxicology
Na-Ra Han, Phil-Dong Moon, Hyung-Min Kim, Hyun-Ja Jeong
OBJECTIVES: Atopic dermatitis (AD) is an allergic and inflammatory skin disorder caused by a combination of itching and skin sensitization by allergens. This article investigated whether cordycepin modulates AD symptoms by using an AD murine model. MATERIAL AND METHODS: We evaluated a regulatory effect and specific molecular mechanism of cordycepin on AD induced by the repeated local exposure of 2,4-dinitrochlorobenzene to dorsal skin of mice. Blood or AD-like skin lesions samples were removed for histopathologic analysis, enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, and Western blot analyses...
September 5, 2018: Immunopharmacology and Immunotoxicology
Rasoul Baharlou, Nader Tajik, Mahdi Behdani, Mohammad Ali Shokrgozar, Vajiheh Tavana, Fatemeh Kazemi-Lomedasht, Fatemeh Faraji, Mahdi Habibi-Anbouhi
OBJECTIVES: Angiogenesis targeting is an attractive approach for cancer treatment. Delta-like ligand 4 (DLL4) plays a pivotal role in neovascular development and its inhibitors have recently entered clinical trials for solid tumors. The aim of this study was to evaluate the possibilities of using anti-DLL4 antibody fragment as an angiogenesis maturation inhibitor. MATERIALS AND METHODS: In this study, a DLL4-specific Nanobody, named 3Nb3, was selected and assessed by western blotting and internalization assays...
September 5, 2018: Immunopharmacology and Immunotoxicology
Ana Paula Fortes Dos Santos Thomazelli, Fernanda Tomiotto-Pellissier, Milena Menegazzo Miranda-Sapla, Suelen Santos da Silva, Daniele Sapede Alvarenga, Carolina Panis, Allan Henrique Depieri Cataneo, Juliano Bordignon, Guilherme Ferreira Silveira, Lucy Megumi Yamauchi, Jussevania Pereira Santos Rubro de Sá, Ionice Felipe, Wander Rogério Pavanelli, Ivete Conchon-Costa
The context of the article: Leishmania amazonensis has a wide geographical distribution throughout South American countries and can cause self-healing to severe cases as mucocutaneous or visceral forms. Leishmaniasis presents a balance of inflammatory and anti-inflammatory cytokines which is responsible for promoting the activation of phagocytes, essential to control the infection and lead to tissue repair/resolution of the disease, respectively. RESULTS AND DISCUSSION: Our model revealed that the treatment with Con-A was capable to stimulate human PBMC cells by increasing the phagocytic capacity and promoting parasite elimination...
September 5, 2018: Immunopharmacology and Immunotoxicology
Yiqing Wang, Qichun Tang, Peibei Duan, Lihua Yang
Ulcerative colitis (UC) is a chronic, relapsing, remitting, and inflammatory disorder that afflicts millions of people around the world. It carries a substantial economic burden, reducing the quality of life, ability to work, and increasing disability. Conventional medical treatment of UC includes the use of aminosalicylates, corticosteroids, and immunosuppressive drugs. However, these medicines are not always effective due to some serious side effects. Nuclear factor-kappa B (NF-κB) is a key factor in the inflammatory setting and strongly affects the course of mucosal inflammation in UC...
August 15, 2018: Immunopharmacology and Immunotoxicology
Geneviève Veereman, Bruno Hauser, Elisabeth De Greef, Thierry Devreker, Koen Huysentruyt, Roel Lemmens, Yvan Vandenplas
Major pharmaceutical advancements in the field of inflammatory bowel diseases benefit to children and adolescents affected with this progressive chronic condition. Scientific organisations such as ESPGHAN and ECCO actively publish guidelines related to the many aspects of care from these patients. Clinical studies and long-term prospective registries in the appropriate age groups are crucial to support an evidence based strategy.
July 24, 2018: Immunopharmacology and Immunotoxicology
Fu-Sheng Gao, Yu-Tao Zhan, Xu-Dong Wang, Chuan Zhang
AIM: DNA vaccines have emerged as a promising strategy for cancer immunotherapy; however, their immunogenicity is weak. Fms-like tyrosine kinase 3-ligand (Flt3L) has been exploited for its ability to increase the proliferation of dendritic cells (DCs). The aim of the present study was to investigate whether co-administration of an adjuvant plasmid expressing mouse Flt3L and a DNA vaccine of the Mucin 1 (MUC1) antigen enhances immune responses. METHODS: The recombinant plasmids pVAX1-MUC1 and pVAX1-Flt3L were constructed and injected into mice intramuscularly (i...
August 2018: Immunopharmacology and Immunotoxicology
Dong Xu, Hongying Zhu, Qingdong Fu, Songxiao Xu, Wen Sun, Guoxiao Chen, Xiaoli Lv
OBJECTIVE: Lens epithelial cell (LEC) membrane damage is one of the pathogenesis of cataract. High mobility group box-1 (HMGB-1) and nuclear factor-κB (NF-κB) play vital roles in a variety of diseases, such as inflammation. Ketamine has numerous pharmacological effects that can inhibit inflammation. However, its role in cataract rats LECs has not yet been elucidated. MATERIALS AND METHODS: LECs were isolated from SD rats and cultured in vitro. The cells were randomly divided into three groups, including the control group, cataract model group induced by H2 O2 , and ketamine group treated by 10 mM ketamine under H2 O2 environment...
August 2018: Immunopharmacology and Immunotoxicology
Wenjun Cao, Guoqiang Hou, Xin Zhang, Hongxia San, Jianzhong Zheng
OBJECTIVE: Gastric polyps (GPs) are masses of cells that form on the lining inside stomach. The diagnosis and treatment of GPs are vital, since some cases may cause malignant. Endoscopic techniques have led to earlier detection of GPs, the incidence of GPs are projected to continue rising. The treatment of GPs and investigation are specific to the possible presentation, pathology, and malignant. This paper is to investigate risk factors related to GPs patients in China. MATERIALS AND METHODS: Total of 2048 patients who underwent gastrointestinal endoscopy from January 2014 to December 2014 were studied...
August 2018: Immunopharmacology and Immunotoxicology
Suat Kamisli, Osman Ciftci, Asli Taslidere, Nese Basak Turkmen, Cemal Ozcan
AIM: The aim of this study was to investigate the beneficial effects of 18β-glycyrrhetinic acid (GA) on the experimental allergic encephalomyelitis (EAE) in C57BL/6 mice. GA is a natural substance found in the root of licorice and is used in traditional Chinese medicine. It has many pharmacological activities such as antioxidant, anti-inflammatory, and anti-cancer effects. MATERIALS AND METHODS: A total of 40 C57BL/6 mice were divided equally into four groups: (1) Control, (2) EAE, (3) GA and (4) GA + EAE...
August 2018: Immunopharmacology and Immunotoxicology
Qiang Wu, Qinliang Xu, Xiangdong Jian, Huaxue Wang, Xiandi He, Beijun Gao, Ke Wang, Baotian Kan
Paraquat (methyl viologen, PQ) is highly toxic to humans. Pulmonary fibrosis is the most common cause of death after PQ poisoning. However, no effective therapy is available. The current treatment dilemma and pathology suggest that we should reconsider how to treat the poisoning using other methods, such as immunization. Some clues indicate that immune mechanisms may play important roles in the pathology of PQ poisoning. We implemented a simple experiment to test the hypothesis that activated innate immunity was involved in acute lung injury induced by PQ...
August 2018: Immunopharmacology and Immunotoxicology
Gui-Mei Yu, Di Liu, Na Yuan, Bao-Hua Liu
Acid-sensing ion channels (ASIC) are voltage-independent cationic channels that open in response to decrease in extracellular pH. Amongst different subtypes, ASIC3 has received much attention in joint inflammatory conditions including rheumatoid arthritis. There have been a number of studies showing that there is an increase in expression of ASIC3 on nerve afferents supplying joints in response to inflammatory stimulus. Accordingly, a number of selective as well as nonselective ASIC3 inhibitors have shown potential in attenuating pain and inflammation in animal models of rheumatoid arthritis...
August 2018: Immunopharmacology and Immunotoxicology
Maryam Mahdavi Siuki, Nafiseh Fazel Nasab, Elham Barati, Maryam Nezafat Firizi, Tahereh Jalilvand, Hasan Namdar Ahmadabad
OBJECTIVE: Berberine is an isoquinoline derivative alkaloid with anti-inflammatory activity. In this study, we investigated the protective effects of berberine in prevention of LPS-induced abortion. MATERIALS AND METHODS: On the gestation day (GD) 9.5, the pregnant mice were injected with low, medium, and high doses of berberine or with PBS. After 4 h, berberine or PBS-pretreated mice were injected with LPS. On GD 11.5, blood samples and uterine tissues were collected from treated mice and percentage of abortion and serum levels of NO, TNF-α, IL-10, and IL12p70 were measured by macroscopic examination and sandwich ELISA, respectively...
August 2018: Immunopharmacology and Immunotoxicology
Tengli Cao, Xiuxiu Zhang, Dingding Chen, Peiyan Zhang, Qing Li, Abbas Muhammad
CONTEXT: CD4 + CD25+ regulatory T (Treg) lymphocytes are critical for immune homeostasis. Foxp3 (Forkhead Box protein P3) is always considered as a marker of function and identities determination of Treg cells because of special occurring in Treg cell. People who lack Treg cells or have a low expression of Foxp3 gene will suffer fatal autoimmunity. Scientists are trying to use Treg cells as a treatment for autoimmune disease, such as systemic lupus erythematosus. OBJECTIVE: Our objective was to induce Foxp3 + CD4+ T cells from naïve CD4 + T cells isolated from C57 mice spleen in vitro using stimuli that include the short chain fatty acid sodium butyrate...
August 2018: Immunopharmacology and Immunotoxicology
Hyun-Ja Jeong, Ka-Jung Ryu, Hyung-Min Kim
OBJECTIVE: Previous studies reported that depletion of Bcl-2 has a protective effect against allergic diseases. Furthermore, recently our study showed that anticancer drug has antiallergic inflammatory effect. An anticancer agent ABT-737 is an inhibitor of Bcl-2 and has an anti-inflammatory effect. However, the antiallergic inflammatory activity of ABT-737 is still unknown. Here, we aimed to explore the anti-atopic dermatitis (AD) activity and the mechanism of ABT-737 in AD models. MATERIALS AND METHODS: HaCaT cells were used for in vitro experiments...
August 2018: Immunopharmacology and Immunotoxicology
Nan Wang, Delu Che, Yingnan Zeng, Jiao Cao, Jue Wang, Tao Zhang
BACKGROUND: Baige (BG) is a compound Chinese herbal preparation, constituted of different position extracts (ethanol extracts from Pueraria lobate and SFE-CO2 extracts from Radix Angelicae dahuricae) of P. lobata and A. dahurica to treat the brain injury in patients. AIM: The goal of this study was to identify the neuroprotective properties of BG and its principal component mixture (PCM) and verify whether the material basis for BG is its PCM. METHODS: Middle cerebral artery occlusion (MCAO) was operated on male Sprague-Dawley rat for 2 h, different doses of BG or PCM or vehicle were gavaged after 3 h of MCAO...
August 2018: Immunopharmacology and Immunotoxicology
Mohammad Taghi Palizgir, Maryam Akhtari, Mahdi Mahmoudi, Shayan Mostafaei, Alireza Rezaiemanesh, Farhad Shahram
OBJECTIVE: Behcet's disease (BD) is an auto-inflammatory disorder. Curcumin as a bio-active agent has anti-inflammatory properties. Effects of curcumin on the pathogenesis of BD are still not clear. In this study, we investigated the effect of curcumin on the inflammatory cytokines expression and production in M1 macrophages from BD patients compared with healthy controls. METHODS: Monocytes were collected from 10 healthy controls and 20 active BD patients, differentiated to macrophages by macrophage-colony stimulating factor for 7 d...
August 2018: Immunopharmacology and Immunotoxicology
Issa Layali, Alireza Shahriary, Nima Rahmani Talatappe, Eisa Tahmasbpour, Hossein Rostami, Asghar Beigi Harchegani
CONTEXT: Sulfur mustard (SM) is a lipophilic and reactive chemical compound that targets human airway system. OBJECTIVE: Glutathione (GSH) depletion, oxidative stress (OS) status, and changes in expression of GSH-dependent antioxidant enzymes were considered in human mustard lungs. MATERIALS AND METHODS: Lung biopsies and bronchoalveolar lavage (BAL) were collected from non-exposed (n = 10) individuals and SM-exposed patients (n = 12)...
August 2018: Immunopharmacology and Immunotoxicology
Chunhong Xiao, Hong Hong, Haizhong Yu, Jianfen Yuan, Chunyan Guo, Hongyan Cao, Weibing Li
OBJECTIVE: Janus kinase (JAK)-signal transducer and activator of transcription (STAT) signaling pathway is closely related to tumorigenesis. Suppressors of cytokine signaling 3 (SOCS3) is a negative regulator of JAK-STAT signaling pathway. MiR-340 expression is significantly upregulated in gastric cancer (GC) tissue. This study investigated the role of miR-340 in regulating SOCS3 expression and affecting GC cell proliferation, cycle, and apoptosis. PATIENTS AND METHODS: Dual luciferase assay was used to verify the targeted relationship between miR-340 and SOCS3...
August 2018: Immunopharmacology and Immunotoxicology
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