journal
Journals Environmental and Molecular Mu...

Environmental and Molecular Mutagenesis

https://read.qxmd.com/read/38525651/investigations-on-the-genotoxic-potential-of-styrene-in-fischer-344-rats-using-multiple-endpoints
#1
JOURNAL ARTICLE
B Bhaskar Gollapudi
Genotoxicity of styrene monomer was evaluated in male Fischer 344 rats using the alkaline comet assay for DNA damage, micronucleus assay for cytogenetic damage and the Pig-a assay for gene mutations. In a dose range finding (DRF) study, styrene was administered by oral gavage in corn oil for 28 consecutive days at 0, 100, 500, and 1000 mg/kg/day. The bioavailability of styrene was confirmed in the DRF by measuring its plasma levels at approximately 7- or 15-min following dosing. The 1000 mg/kg/day group exceeded the maximum tolerated dose based on body weight and organ weight changes and signs of central nervous system depression...
March 25, 2024: Environmental and Molecular Mutagenesis
https://read.qxmd.com/read/38523463/qualitative-and-quantitative-changes-in-mitochondrial-dna-associated-with-cervical-cancer-a-comprehensive-review
#2
REVIEW
Isadora Oliveira Ansaloni Pereira, Nayara Nascimento Toledo Silva, Angelica Alves Lima, Glenda Nicioli da Silva
Cervical cancer is the fourth most commonly diagnosed cancer in women and is considered a preventable disease, as vaccination and screening programs effectively reduce its incidence and mortality rates. Disease physiopathology and malignant cell transformation is a complex process, but it is widely known that high-risk HPV (hrHPV) infection is a necessary risk factor for cancer development. Mitochondria, cell organelles with important bioenergetic and biosynthetic functions, are important for cell energy production, cell growth, and apoptosis...
March 24, 2024: Environmental and Molecular Mutagenesis
https://read.qxmd.com/read/38523457/firefighting-per-and-polyfluoroalkyl-substances-and-dna-methylation-of-genes-associated-with-prostate-cancer-risk
#3
JOURNAL ARTICLE
Margaret Quaid, Jaclyn M Goodrich, Miriam M Calkins, Judith M Graber, Derek Urwin, Jamie Gabriel, Alberto J Caban-Martinez, Rebekah L Petroff, Casey Grant, Shawn C Beitel, Sally Littau, John J Gulotta, Darin Wallentine, Jeff Hughes, Jefferey L Burgess
Prostate cancer is the leading incident cancer among men in the United States. Firefighters are diagnosed with this disease at a rate 1.21 times higher than the average population. This increased risk may result from occupational exposures to many toxicants, including per- and polyfluoroalkyl substances (PFAS). This study assessed the association between firefighting as an occupation in general or PFAS serum levels, with DNA methylation. Only genomic regions previously linked to prostate cancer risk were selected for analysis: GSTP1, Alu repetitive elements, and the 8q24 chromosomal region...
March 24, 2024: Environmental and Molecular Mutagenesis
https://read.qxmd.com/read/38465801/urinary-mutagenicity-and-bladder-cancer-risk-in-northern-new-england
#4
JOURNAL ARTICLE
Jason Y Y Wong, Alexander H Fischer, Dalsu Baris, Laura E Beane Freeman, Margaret R Karagas, Molly Schwenn, Alison Johnson, Peggy P Matthews, Adam E Swank, G Monawar Hosain, Stella Koutros, Debra T Silverman, David M DeMarini, Nathaniel Rothman
The etiology of bladder cancer among never smokers without occupational or environmental exposure to established urothelial carcinogens remains unclear. Urinary mutagenicity is an integrative measure that reflects recent exposure to genotoxic agents. Here, we investigated its potential association with bladder cancer in rural northern New England. We analyzed 156 bladder cancer cases and 247 cancer-free controls from a large population-based case-control study conducted in Maine, New Hampshire, and Vermont...
March 11, 2024: Environmental and Molecular Mutagenesis
https://read.qxmd.com/read/38385761/genotoxicity-in-humans-exposed-to-arsenic-lithium-and-boron-in-drinking-water-in-the-bolivian-andes-a-cross-sectional-study
#5
JOURNAL ARTICLE
Noemi Tirado, Josué Mamani, Jessica De Loma, Franz Ascui, Karin Broberg, Jacques Gardon
Elevated concentrations of arsenic, lithium and boron in drinking water have already been reported in Bolivia. Arsenic is known to cause genotoxicity but that caused by lithium and boron is less well known. The aim of the present cross-sectional study was to evaluate potential genotoxic effects of exposure to arsenic, while considering exposure to lithium and boron and genetic susceptibility. Women (n = 230) were recruited in villages located around Lake Poopó. Exposure to arsenic was determined as the sum of concentrations of arsenic metabolites inorganic arsenic, monomethylarsonic acid (MMA), and dimethylarsinic acid (DMA) in urine...
February 22, 2024: Environmental and Molecular Mutagenesis
https://read.qxmd.com/read/38353119/cytogenotoxicity-effects-in-addicts-with-multidrug-consumption
#6
JOURNAL ARTICLE
Ana Elizabeth González-Santiago, Alejandro Salvador Gómez-Cabrera, Raúl Cuauhtémoc Baptista-Rosas, Guillermo Moisés Zúñiga-González, Belinda Claudia Gómez-Meda, Ana Alondra Sobrevilla Navarro, María Guadalupe Sánchez-Parada
Drug abuse is considered a global health problem with serious social impact. In recent decades, changes in drug consumption patterns have shown a clear rising trend in the use of multiple drugs. Although the buccal micronucleus cytome (BMCyt) assay has evaluated cytotoxicity in drug abuse, there has not been an approach that takes into account this pattern of multiple drug use. Therefore, in this study, we evaluate for the first time the cytogenotoxic effects in multidrug users, and its correlation with the amount consumed and years of abuse...
February 14, 2024: Environmental and Molecular Mutagenesis
https://read.qxmd.com/read/38333941/veterinarians-exposed-to-inhaled-anesthetic-present-chromosome-damage-apoptosis-and-cell-cycle-changes
#7
JOURNAL ARTICLE
Mariana G Braz, Drielle B S Figueiredo, Marjorie A Golim, Tony F Grassi, Bruno R B da Costa, Bruno S De Martinis, Leandro G Braz
This cross-sectional study evaluated, for the first time, DNA damage, viability, and cell death of lymphocytes and cell cycle phases of mononuclear and polymorphonuclear cells in veterinarians exposed to the volatile anesthetic isoflurane. Veterinarians who were occupationally exposed to isoflurane (exposed group; n = 20) and matched-unexposed individuals (volunteers without occupational exposure; n = 20) were enrolled in the study. DNA damage was assessed in lymphocytes by micronucleus (MN) and phosphorylated histone gamma-H2AX (γ-H2AX)...
February 9, 2024: Environmental and Molecular Mutagenesis
https://read.qxmd.com/read/38333939/comparative-analysis-of-micronucleus-induction-and-dna-damage-biomarkers-in-tk6-and-a375-cells-using-flow-cytometry
#8
JOURNAL ARTICLE
Xiaowen Sun, Richard A Spellman, Maria Engel, Elizabeth Rubitski, Maik Schuler
Previously, we introduced an alternative adherent A375 cell line for clastogenicity and aneugenicity testing using a high content imaging platform. To further characterize the performance of A375 cells, we investigated the sensitivity and specificity of A375 and TK6 cells by directly comparing micronucleus (MN) induction, cytotoxicity (relative cell counts, viability, and apoptosis), clastogenicity (γH2AX), and aneuploidy markers (pH 3, MPM-2, and polyploidy) using flow cytometric methods. We evaluated 14 compounds across different mechanisms (non-genotoxic apoptosis inducers, clastogens, and aneugens with either tubulin binding or aurora kinase inhibiting phenotypes) at 4-h and 24-h post treatment...
February 9, 2024: Environmental and Molecular Mutagenesis
https://read.qxmd.com/read/38299759/effect-of-smoking-on-methylation-and-semen-parameters
#9
JOURNAL ARTICLE
Nasim Naeimi, Homa Mohseni Kouchesfehani, Zahra Heidari, Hamidreza Mahmoudzadeh-Sagheb
One type of epigenetic modification is genomic DNA methylation, which is induced by smoking, and both are associated with male infertility. In this study, the relationship between smoking and CHD5 gene methylation and semen parameters in infertile men was determined. After the MS-PCR of blood in 224 samples, 103 infertile patients (62 smokers and 41 non-smokers) and 121 fertile men, methylation level changes between groups and the effect of methylation and smoking on infertility and semen parameters in infertile men were determined...
February 1, 2024: Environmental and Molecular Mutagenesis
https://read.qxmd.com/read/38115239/interpretation-of-in-vitro-concentration-response-data-for-risk-assessment-and-regulatory-decision-making-report-from-the-2022-iwgt-quantitative-analysis-expert-working-group-meeting
#10
REVIEW
Marc A Beal, Guangchao Chen, Kerry L Dearfield, Min Gi, Bhaskar Gollapudi, Robert H Heflich, Katsuyoshi Horibata, Alexandra S Long, David Lovell, Barbara L Parsons, Stefan Pfuhler, John Wills, Andreas Zeller, George Johnson, Paul A White
Quantitative risk assessments of chemicals are routinely performed using in vivo data from rodents; however, there is growing recognition that non-animal approaches can be human-relevant alternatives. There is an urgent need to build confidence in non-animal alternatives given the international support to reduce the use of animals in toxicity testing where possible. In order for scientists and risk assessors to prepare for this paradigm shift in toxicity assessment, standardization and consensus on in vitro testing strategies and data interpretation will need to be established...
December 19, 2023: Environmental and Molecular Mutagenesis
https://read.qxmd.com/read/37984337/the-aryl-hydrocarbon-receptor-ahr-activation-mediates-benzo-a-pyrene-induced-overexpression-of-aqp3-and-notch1-in-hacat-cells
#11
JOURNAL ARTICLE
Claudia I Almendarez-Reyna, Carlos Gabriel de la Trinidad Chacón, Ángeles C Ochoa-Martínez, Luis A Rico-Guerrero, Iván N Pérez-Maldonado
The aim of this study was twofold: 1) evaluate the effect of benzo[a]pyrene (BaP) on expression levels of AQP3 and Notch1 genes in HaCaT cells exposed "in vitro" and 2) investigate the possible biological role of assessed genes by bioinformatics methods. Cells were exposed to increasing concentrations of BaP (0.0 - 4.0 μM) for 1-4 days. After treatments, cell viability and expression levels of AhR, CYP1A1, AQP3, and Notch1 genes were evaluated. The possible biological role of assessed genes was evaluated using bioinformatics tools...
November 20, 2023: Environmental and Molecular Mutagenesis
https://read.qxmd.com/read/37957787/clonality-trafficking-and-molecular-alterations-among-hprt-mutant-t-lymphocytes-isolated-from-control-mice-versus-mice-treated-with-n-ethyl-n-nitrosourea
#12
JOURNAL ARTICLE
Stephen A Judice, Hillary E Sussman, Dale M Walker, J Patrick O'Neill, Richard J Albertini, Vernon E Walker
Mutations in T lymphocytes (T-cells) are informative quantitative markers for environmental mutagen exposures, but risk extrapolations from rodent models to humans also require understanding how T-cell development and proliferation kinetics impact mutagenic outcomes. Rodent studies have shown that patterns in chemical-induced mutations in the hypoxanthine-guanine phosphoribosyltransferase (Hprt) gene of T-cells differ between lymphoid organs. The current work was performed to obtain knowledge of the relationships between maturation events during T-cell development and changes in chemical-induced mutant frequencies over time in differing immune compartments of a mouse model...
November 13, 2023: Environmental and Molecular Mutagenesis
https://read.qxmd.com/read/37942839/in-vivo-genotoxicity-testing-strategies-report-from-the-8th-international-workshop-on-genotoxicity-testing-iwgt
#13
REVIEW
Carol Beevers, Yoshifumi Uno, Krista Meurer, Shuichi Hamada, Kiyohiro Hashimoto, David Kirkland, Matthew J LeBaron, Frank Le Curieux, Ludovic Le Hegarat, Hans-Joerg Martus, Kenichi Masumura, Wakako Ohyama, Daniel J Roberts, Marie Vasquez, James Whitwell, Kristine L Witt
The in vivo working group (WG) considered three topics: acceptable maximum doses for negative erythrocyte micronucleus (MN) tests; validation status of MN assays in non-haematopoietic tissues; nuisance factors in the comet assay. The WG reached agreement on many issues, including: Negative erythrocyte MN studies should be acceptable if dosing is conducted to OECD test guideline (TG) 474 recommendations and if sufficient bone marrow exposure is demonstrated; consensus on the evidence required to demonstrate "sufficient" exposure was not reached...
November 9, 2023: Environmental and Molecular Mutagenesis
https://read.qxmd.com/read/37926486/the-pig-a-gene-mutation-assay-in-human-biomonitoring-and-disease
#14
REVIEW
Rachel Lawrence, Kathryn Munn, Hamsa Naser, Laura Thomas, Hasan Haboubi, Lisa Williams, Shareen Doak, Gareth Jenkins
The blood cell phosphatidylinositol glycan class A (PIG-A) gene mutation assay has been extensively researched in rodents for in vivo mutagenicity testing and is now being investigated in humans. The PIG-A gene is involved in glycosyl phosphatidylinositol (GPI)-anchor biosynthesis. A single mutation in this X-linked gene can lead to loss of membrane bound GPI-anchors, which can be enumerated via corresponding GPI-anchored proteins (e.g., CD55) using flow cytometry. The studies published to date by different research groups demonstrate a remarkable consistency in PIG-A mutant frequencies...
November 5, 2023: Environmental and Molecular Mutagenesis
https://read.qxmd.com/read/37916278/assessment-of-the-in-vivo-genotoxic-potential-of-three-smoke-flavoring-primary-product-mixtures
#15
JOURNAL ARTICLE
Chad M Thompson, Gregory Brorby, Zena Keig-Shevlin, Robert Smith, Allison Franzen, Kristina Ulrich, Alexander D Blanchette, Candace Doepker
Smoke flavorings are mixtures generated from wood pyrolysis that are filtered to remove tar and are often considered healthier alternatives to conventional smoking processes. While the latter is mostly unregulated, smoke flavoring primary products (SFPPs) are undergoing the 10-year required re-evaluation in the European Union (EU). To comply with recent smoke flavor guidance, in vivo micronucleus studies in rats and transgenic rodent (TGR) mutation assays in MutaTM Mice were conducted on three SFPPs. For most studies, typical limit doses were exceeded to comply with regulatory requests...
November 2, 2023: Environmental and Molecular Mutagenesis
https://read.qxmd.com/read/37704589/genotoxicity-assessment-in-heparg%C3%A2-cells-as-a-new-approach-methodology-follow-up-to-a-positive-response-in-the-human-tk6-cell-micronucleus-assay-naphthalene-case-study
#16
LETTER
Leslie Recio, Jasmine Fowler, Lincoln Martin, Carol Swartz
We are evaluating the use of metabolically competent HepaRG™ cells combined with CometChip® for DNA damage and the micronucleus (MN) assay as a New Approach Methodology (NAM) alternative to animals for follow up genotoxicity assessment to in vitro positive genotoxic response. Naphthalene is genotoxic in human TK6 cells inducing a nonlinear dose-response for the induction of micronuclei in the presence of rat liver S9. of naphthalene. In HepaRG™ cells, naphthalene genotoxicity was assessed using either 6 (CometChip™) or 12 concentrations of naphthalene (MN assay) with the top dose used for assessment of genotoxicity for the Comet andMN assay was 1...
September 13, 2023: Environmental and Molecular Mutagenesis
https://read.qxmd.com/read/37658772/the-association-of-epidermal-growth-factor-variant-with-oral-squamous-cell-carcinoma
#17
JOURNAL ARTICLE
Baris Ertugrul, Amal Mohammad, Goksu Kasarci, Sinem Bireller, Murat Ulusan, Bedia Cakmakoglu
In this study, our aim was to investigate the epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) gene polymorphisms in oral squamous cell carcinoma (OSCC) patients and non-OSCC healthy controls. This case-control study comprised 89 OSCC and 107 healthy controls by using polymerase chain reaction (PCR) and restriction-fragment-length-polymorphism methods, the genotypes for EGF +61 A>G (rs4444903) and EGFR R497K (rs2227983) were analyzed. According to the EGF +61 A>G genotype distribution, individuals with the GG genotype were more prevalent in the OSCC group when compared to the healthy controls...
September 2, 2023: Environmental and Molecular Mutagenesis
https://read.qxmd.com/read/37650425/abstracts-from-the-54th-annual-meeting-of-the-environmental-mutagenesis-and-genomics-society-september-9-13-2023-chicago-il-usa-emgs-in-the-windy-city-billowing-the-sails-of-dna-science
#18
JOURNAL ARTICLE
(no author information available yet)
No abstract text is available yet for this article.
September 2023: Environmental and Molecular Mutagenesis
https://read.qxmd.com/read/37606505/oxidative-dna-damage-on-the-vegf-g-quadruplex-forming-promoter-is-repaired-via-long-patch-ber
#19
JOURNAL ARTICLE
Adil S Hussen, Haley L Kravitz, Bret D Freudenthal, Amy M Whitaker
In response to oxidative damage, base excision repair (BER) enzymes perturb the structural equilibrium of the VEGF promoter between B-form and G4 DNA conformations, resulting in epigenetic-like modifications of gene expression. However, the mechanistic details remain enigmatic, including the activity and coordination of BER enzymes on the damaged G4 promoter. To address this, we investigated the ability of each BER factor to conduct its repair activity on VEGF promoter G4 DNA substrates by employing pre-steady-state kinetics assays and in vitro coupled BER assays...
August 22, 2023: Environmental and Molecular Mutagenesis
https://read.qxmd.com/read/37554110/genome-wide-impact-of-cytosine-methylation-and-dna-sequence-context-on-uv-induced-cpd-formation
#20
JOURNAL ARTICLE
Hannah E Wilson, John J Wyrick
Exposure to ultraviolet (UV) light is the primary etiological agent for skin cancers because UV damages cellular DNA. The most frequent form of UV damage is the cyclobutane pyrimidine dimer (CPD), which consists of covalent linkages between neighboring pyrimidine bases in DNA. In human cells, the 5' position of cytosine bases in CG dinucleotides is frequently methylated, and methylated cytosines in the TP53 tumor suppressor are often sites of mutation hotspots in skin cancers. It has been argued that this is because cytosine methylation promotes UV-induced CPD formation; however, the effects of cytosine methylation on CPD formation are controversial, with conflicting results from previous studies...
August 9, 2023: Environmental and Molecular Mutagenesis
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