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Toxicology in Vitro: An International Journal Published in Association with BIBRA

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https://www.readbyqxmd.com/read/30453006/bromelain-with-peroxidase-from-pineapple-are-more-potent-to-target-leukemia-growth-inhibition-a-comparison-with-only-bromelain
#1
Rahul Debnath, Nabanita Chatterjee, Subhadip Das, Snehasis Mishra, Dipayan Bose, Somenath Banerjee, Sujata Das, Krishna Das Saha, Durgadas Ghosh, Debasish Maiti
The natural anti-cancer agent bromelain is found to be beneficial for either single or multi-targeted therapy in gastric and skin carcinoma, by inhibiting cancer cell growth. Importantly, the presence of peroxidase enhances its biological efficiency. We have now evaluated a panel of cancer cell lines with bromelain in presence or absence of peroxidase to identify that the combination has higher apoptosis inducing potential in all those cell lines. Bromelain plus peroxidase (BM-PR) inhibited acute myeloid (K562) cell proliferation and altered the morphological features...
November 16, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30448557/poly-adp-ribose-polymerase-1-promotes-expression-of-mir-155-by-the-up-regulation-of-methyl-cpg-binding-domain-protein-2-in-tk6-cells-exposed-to-hydroquinone
#2
Zhiming Gui, Haiqiao Zhang, Tan Qiang, Xiaoxuan Ling, Zhidong Liu, Jianming Peng, Junli Shao, Minhua Wu, Qian Yuan, Jieyou Li, Zhijie Pan, Baisen Zhong, Linhua Liu
Hydroquinone (HQ), one of the major metabolites of benzene, can induce aberrant gene expression. MiR-155, a tumor activator, participates in various biological processes, including DNA damage response. However, the molecular mechanism of aberrant miR-155 expression is still not completely elucidated. Here, we investigated the mechanism of abnormal expression of miR-155 induced by poly(ADP-ribose)polymerase-1 (PARP-1) expression in HQ-treated TK6 lymphoblastoid cells. We examined the expression of genes related to abnormal expression of miR-155 to explore the reason for this phenomenon...
November 15, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30448556/overexpression-of-hif-1a-could-partially-protect-k562-cells-from-1-4-benzoquinone-induced-toxicity-by-inhibiting-ros-apoptosis-and-enhancing-glycolysis
#3
Rongli Sun, Xing Meng, Yunqiu Pu, Fengxia Sun, Zhaodi Man, Juan Zhang, Lihong Yin, Yuepu Pu
Benzene is an environmental contaminant which causes hematological diseases. Previously, hypoxia inducible factor-1a (HIF-1a) was found to be involved in benzene-induced hematotoxicity. This study aims to explore whether overexpression of HIF-1a in K562 cell line could influence the toxicity caused by 1,4-BQ. HIF-1a overexpression K562 cell line was constructed with a lentiviral vector. Results showed that HIF-1a was significantly elevated in control K562 cells and HIF-1a overexpression cells exposed to 1,4-BQ...
November 15, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30448555/mdma-modulates-5-ht-1-mediated-contractile-response-of-the-human-internal-thoracic-artery-in-vitro
#4
Diogo A Fonseca, Cristina Teixeira, Félix Carvalho, Eduarda Fernandes, Pedro E Antunes, Manuel J Antunes, Maria Dulce Cotrim
3,4-Methylenedioxymethamphetamine (MDMA or "ecstasy") is a popular recreational drug of abuse. In addition to its characteristic psychotropic effects, important cardiovascular effects have been described such as increased blood pressure and heart rate. MDMA was previously shown to behave as a partial agonist on 5-hydroxytryptamine (5-HT) receptors in the human internal thoracic artery in vitro, involving the 5-HT2A subtype. Here, we studied the interaction of MDMA (400, 800 and 1600 μM) with the following 5-HT receptor agonists: 5-carboxamidotryptamine (5-CT, full agonist for the 5-HT1 , 5-HT2 , 5-HT5 , 5-HT6 and 5-HT7 receptors) and sumatriptan (selective 5-HT1B/1D receptors agonist)...
November 15, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30447388/methylglyoxal-disturbs-the-expression-of-antioxidant-apoptotic-and-glycation-responsive-genes-and-triggers-programmed-cell-death-in-human-leukocytes
#5
Alessandro de Souza Prestes, Matheus Mulling Dos Santos, Assis Ecker, Gabriel Teixeira de Macedo, Roselei Fachinetto, Getulio Nicola Bressan, João Batista Teixeira da Rocha, Nilda Vargas Barbosa
Methylglyoxal (MG) is a α-dycarbonyl compound derived mainly from glycolysis, whose accumulation is harmful for cells and tissues. Here, we evaluated the cytotoxic effects induced by MG in leukocytes after an acute exposure, measuring as endpoints of toxicity some markers of oxidative stress and programmed cell death. Human leukocytes were isolated and incubated with MG at concentrations ranging from 0.1 to 10 mM for 2.5 h, and subsequently prepared for assays based in flow cytometry, gene expression and immunoreactivity profile...
November 14, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30439410/intra-axonal-ca-2-mobilization-contributes-to-triphenyltin-induced-facilitation-in-glycinergic-transmission-of-rat-spinal-neurons
#6
Kazuki Noma, Yuki Kurauchi, Hiroshi Katsuki, Yasuo Oyama, Norio Akaike
Triphenyltin (TPT) is an organotin compound causing environmental hazard to many wild creatures. Our previous findings show that TPT increases of the frequency of spontaneous glycinergic inhibitory postsynaptic currents (sIPSCs) in rat spinal neurons without changing the amplitude and 1/e decay time. In our study, the effects of 2-aminoethoxydiphenyl borate (2-APB), dantrolene sodium, and thapsigargin on sIPSC frequency were examined to reveal the contribution of intra-axonal Ca2+ mobilization by adding TPT...
November 12, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30414920/hyphae-fragments-from-a-fumigatus-sensitize-lung-cells-to-silica-particles-min-u-sil-increased-release-of-il-1%C3%AE
#7
E Øya, F D Zegeye, A K Bølling, R Øvstebø, A K J Afanou, J Øvrevik, M Refsnes, J A Holme
Exposure to particulate matter (PM), such as mineral particles and biological particles/components may be linked to aggravation of respiratory diseases, including asthma. Here we report that exposure to Aspergillus fumigatus hyphae fragments (AFH) and lipopolysaccharide (LPS) induced both mRNA synthesis and release of pro-inflammatory interleukin-1 beta (IL-1β) in both human THP-1 monocytes (THP-1 Mo) and phorbol 12-myristate 13-acetate (PMA)-differentiated THP-1 monocytes (THP-1 macrophages; THP-1 Ma); while Min-U-Sil alone enhanced the release of IL-1β only in THP-1 Ma...
November 8, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30389606/comparative-cytotoxicity-of-plant-protection-products-and-their-active-ingredients
#8
Sarah Adler-Flindt, Sabine Martin
Toxicological testing of plant protection products (PPPs) is a legal requirement in the EU. The whole PPP formulation is tested for acute endpoints in vivo during approval procedure of PPPs. However, alternative methods such as the CLP calculation method (CM) are employed increasingly. In the first part of this study we analysed PPPs for the correlation of GHS classifications resulting mainly from in vivo LD50 -values with classifications obtained from calculated LD50 -values using the CM. Accordingly, the CM predicted 80% of the PPPs correctly...
October 31, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30389604/danthron-suppresses-autophagy-and-sensitizes-pancreatic-cancer-cells-to-doxorubicin
#9
Hua Chen, Chunle Zhao, Ruizhi He, Min Zhou, Yuhui Liu, Xingjun Guo, Min Wang, Feng Zhu, Renyi Qin, Xu Li
In contrast to the steady increase in survival observed for most cancer types, advances have been slow for pancreatic cancers. Current chemotherapy has limited benefits for patients with pancreatic cancer. Therefore, there is an urgent need for effective pancreatic cancer treatment strategies. At present, targeting the autophagic pathway is regarded as a promising new strategy for cancer treatment. Danthron (1,8-dihydroxyanthrquinone), a component from Rheum palmatum L. (polygonaceae), has several biological activities...
October 31, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30389605/antiproliferative-and-proapoptotic-activities-of-aza-annulated-naphthoquinone-analogs
#10
Cristian Suárez-Rozas, Sebastián Simpson, Sebastián Fuentes-Retamal, Mabel Catalán, Jorge Ferreira, Cristina Theoduloz, Jaime Mella, David Cabezas, Bruce K Cassels, Claudia Yáñez, Vicente Castro-Castillo
1,4-Naphthoquinone derivatives have been widely documented with regard to their biological properties, and particularly their anticancer activities. In the 9,10-anthraquinone family, aza-annulation involving one of the carbonyl oxygen atoms has afforded more potent, possibly less toxic analogues. We recently carried out different modifications on the naphthoquinone skeleton to generate 3-chloro-2-amino- and 3-chloro-2-(N-acetamido)-1,4-naphthoquinone and 3,4-dihydrobenzo[f]quinoxalin-6(2H)-one derivatives. These three series of compounds were now tested against normal human fibroblasts and six human cancer cell lines...
October 30, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30389603/anticancer-mechanism-of-troxerutin-via-targeting-nrf2-and-nf-%C3%AE%C2%BAb-signalling-pathways-in-hepatocarcinoma-cell-line
#11
Nisha Susan Thomas, Kiran George, Athavan Alias Anand Selvam
Troxerutin (TX), a bioflavonoid widely present in various fruits and vegetables, has shown to exhibit numerous pharmacological properties including anti-neoplastic and anti-cancer activities. Nrf2 and NF-κB are the key transcription factors that regulate oxidative stress and inflammation, therefore we assessed whether TX modulate these pathways and its downstream proteins in HuH-7 hepatocarcinoma cells. TX induced apoptotic cellular and nuclear changes were examined by fluorescence staining techniques, agarose gel electrophoresis and flow cytometry...
October 30, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30389602/copper-induces-oxidative-stress-and-apoptosis-through-mitochondria-mediated-pathway-in-chicken-hepatocytes
#12
Fan Yang, Ruonan Pei, Zhuowei Zhang, Jianzhao Liao, Wenlan Yu, Na Qiao, Qingyue Han, Ying Li, Lianmei Hu, Jianying Guo, Jiaqiang Pan, Zhaoxin Tang
The aim of this study was to investigate the effects of excessive copper (Cu)-induced cytotoxicity on oxidative stress and mitochondrial apoptosis in chicken hepatocytes. Chicken hepatocytes were cultured in medium in the absence and presence of copper sulfate (CuSO4 ) (10, 50, 100 μM), in N-acetyl-L-cysteine (NAC) (1 mM), and the combination of CuSO4 and NAC for 24 h. Morphologic observation and function, reactive oxygen species (ROS) level, antioxidant indices, nitric oxide (NO) content, mitochondrial membrane potential (MMP), and apoptosis-related mRNA and protein levels were determined...
October 30, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30385350/mechanisms-of-synergistic-neurotoxicity-induced-by-two-high-risk-pesticide-residues-chlorpyrifos-and-carbofuran-via-oxidative-stress
#13
Dong-Jun Fu, Ping Li, Jian Song, Sai-Yang Zhang, Han-Zhong Xie
Multi-component pesticide residues, especially pesticide residues with synergistic toxicity, are a serious threat to food safety. With risk assessment, we found that Chlorpyrifos (CPF) and Carbofuran (CBF) are 2 pesticide residues with highest risk for Actinidia chinensis planch. The results showed CPF and CBF have a synergistic neurotoxicity on neural cell SK-N-SH. The toxicity was partly depending on oxidative stress (OS) and had effects on cell apoptosis and cell cycle arrest. Furthermore, the toxicity remained on long-term low-dose condition...
October 30, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30385349/phosphorylation-of-eif2%C3%AE-promotes-cell-survival-in-response-to-benzo-a-pyrene-exposure
#14
Evelyn Smit, Jos C S Kleinjans, Twan van den Beucken
Cellular adaptation is important to cope with various stresses induced by altered environmental conditions. By controlling mRNA translation rates cells may adapt to stress to promote survival. Phosphorylation of eIF2α at serine 51 is one of the pathways controlling mRNA translation. Here we investigated the role of phosphorylated eIF2α during exposure to the environmental carcinogen benzo(a)pyrene (BaP). For our study we used mouse embryonic fibroblasts with a wild type eIF2α (MEF WT) and mouse embryonic fibroblasts with an eIF2α S51A knock-in mutation that cannot be phosphorylated...
October 29, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30416090/bleomycin-induced-genotoxicity-in-vitro-in-human-peripheral-blood-lymphocytes-evidenced-as-complex-chromosome-and-chromatid-type-aberrations
#15
Aswathy S Lal, S Kauser Begum, Sraddha S Bharadwaj, Lalitha V, Vijayalakshmi J, Solomon F D Paul, Ravi Maddaly
Bleomycin is a chemotherapeutic and a radiomimetic drug which induces single and double-strand breaks in DNA by forming free radicals. We demonstrate in this study the capacity of bleomycin in inducing complex chromosome- and chromatid-type aberrations. Human peripheral blood was exposed to different concentrations of bleomycin (0, 10, 20, 30 and 40 μg/mL) and the aberrations induced were studied. The chromosomal-type aberrations studied were dicentrics, tricentrics, tetracentrics, centric rings and acentric fragments...
October 26, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30367931/methamphetamine-exposure-induces-neuropathic-protein-%C3%AE-amyloid-expression
#16
Lingling Chen, Pengfei Yu, Li Zhang, Yuxia Zou, Yujuan Zhang, Lei Jiang, Rong Gao, Hang Xiao, Yanning Qian, Jun Wang
Methamphetamine (METH) abusing contributes to dopaminergic neurons degeneration, resulting inParkinson's disease (PD)-like changes. More recently, the association between METH exposure and the Alzheimer's disease (AD)-like changes gained more attention, however, the underlying mechanisms remain poorly understood. In the present study, we aimed to investigate whether METH exposure promotes the formation of Aβ42 , one of the key AD-like pathological proteins. With the cell model PC-12 cell line, it showed that METH treatment significantly increased the level of the precursor protein APP and its hydrolysates CTFs expression in a dose-dependent manner...
October 24, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30366057/primary-and-secondary-pyrrolic-metabolites-of-pyrrolizidine-alkaloids-form-dna-adducts-in-human-a549-cells
#17
Xiaobo He, Qingsu Xia, Qiangen Wu, William H Tolleson, Ge Lin, Peter P Fu
Humans and animals can be exposed to carcinogenic pyrrolizidine alkaloids (PAs) through consumption of plants commonly found in many parts of the world. Although the liver is the primary target organ for carcinogenic PAs, they have also induced lung tumors in rodents. Hepatic cytochrome P450 activity converts PAs into dehydro-PAs that can be hydrolyzed to dehydropyrrolizidine (DHP); these reactive pyrrolic metabolites can produce four characteristic DNA adducts associated with PA-induced liver tumor initiation in laboratory animals...
October 24, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30359721/circulatory-heavy-metals-cadmium-lead-mercury-and-chromium-inversely-correlate-with-plasma-gst-activity-and-gsh-level-in-copd-patients-and-impair-nox4-nrf2-gclc-gst-signaling-pathway-in-cultured-monocytes
#18
Kabita Gogoi, Prasenjit Manna, Tapan Dey, Jatin Kalita, Bala Gopalan Unni, Dibyajyoti Ozah, Pranab Kumar Baruah
This study aims to examine the hypothesis that circulatory heavy metals may be associated with lung function decline and lower plasma GST activity and GSH level in COPD patients via activating monocytes mediated by impairing the NOX4/Nrf2/GCLC/GST signaling pathway. Results showed that the blood levels of heavy metals (cadmium, lead, mercury, and chromium) were significantly higher in COPD patients of coal mine site compared to the healthy controls. The levels of heavy metals in COPD patients were significantly and negatively correlated with lung function, GST activity, and GSH level...
October 22, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30359720/cellular-apoptosis-of-hff-cells-by-inorganic-nanoparticles-not-susceptible-to-modulation-by-toxoplasma-gondii-infection-in-vitro
#19
Oluyomi Stephen Adeyemi, David Adeiza Otohinoyi, Oluwakemi Josephine Awakan, Adebukola Anne Adeyanju
The interaction of nanoparticles with living cells is becoming one of the urgent areas of collaborative research in materials science and biology. Previously, we showed that nanoparticles have promising anti-Toxoplasma gondii properties. Meanwhile, Toxoplasma gondii has been shown to avert apoptosis in host cells whereas nanoparticles have been implicated for apoptotic tendency. Therefore, in the present study, we assessed the in vitro apoptotic properties of inorganic nanoparticles in the absence or presence of Toxoplasma infection and/or small molecules used as metabolic modulators...
October 22, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30347232/further-studies-of-the-efficacy-of-military-commercial-and-novel-skin-decontaminants-against-the-chemical-warfare-agents-sulphur-mustard-soman-and-vx
#20
Hazem Matar, Shirley C Price, Robert P Chilcott
BACKGROUND/AIMS: Following an incident involving toxic chemicals, deployment of countermeasures before the arrival of specialised services at the scene may provide a "therapeutic" window in which to mitigate skin absorption. METHODS: Five potential candidates (itaconic acid, N,N'-methylenebisacrylamide, 2-trifluoromethylacrylic acid, fuller's earth and Fast-Act®) previously found effective against a simulant (methyl salicylate) were evaluated against a 10 μL droplet of 14 C-sulphur mustard (HD), soman (GD) or VX applied to the surface of porcine skin mounted on static skin diffusion cells...
October 19, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
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