journal
MENU ▼
Read by QxMD icon Read
search

Toxicology in Vitro: An International Journal Published in Association with BIBRA

journal
https://www.readbyqxmd.com/read/30016653/in-vitro-potency-determination-of-botulinum-neurotoxin-serotype-a-based-on-its-receptor-binding-and-proteolytic-characteristics
#1
Heike A Behrensdorf-Nicol, Emina Wild, Ursula Bonifas, Jolanta Klimek, Kay-Martin Hanschmann, Beate Krämer, Birgit Kegel
Botulinum neurotoxins (BoNTs) inhibit the release of the neurotransmitter acetylcholine from motor neurons, resulting in highly effective muscle relaxation. In clinical and aesthetic medicine, serotype BoNT/A, which is most potent for humans, is widely used to treat a continuously increasing spectrum of disorders associated with muscle overactivity. Because of the high toxicity associated with BoNTs, it is mandatory to precisely determine the potency of every batch produced for pharmaceutical purposes. Here we report a new quantitative functional in vitro assay for BoNT/A...
July 14, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30016652/para-tertiary-butyl-catechol-induces-eryptosis-in-vitro-via-oxidative-stress-and-hemoglobin-leakage-in-human-erythrocytes
#2
Gopalapura J Vishalakshi, Mahadevappa Hemshekhar, Kempaiah Kemparaju, Kesturu S Girish
Exposure of human population to industrial chemicals is believed as a significant contributing factor to the outgrowth of occupational diseases especially in developing countries due to improper safety measures and sanitary conditions. Para-tertiary-butylcatechol (PTBC) widely employed in petrochemical, thermofax and phototypesetting industries, induces melanocytotoxicity and contact dermatitis leading to occupational leukoderma/vitiligo. Few vitiligo patients were reported for oxidative stress-induced hemolytic anemia and thrombocytopenia, however its impact on blood components is still not clear...
July 14, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30012480/apoptotic-and-antiproliferative-effects-of-stigmast-5-en-3-ol-from-dendronephthya-gigantea-on-human-leukemia-hl-60-and-human-breast-cancer-mcf-7-cells
#3
I P Shanura Fernando, K K Asanka Sanjeewa, Yong-Seok Ann, Chang-Ik Ko, Seung-Hong Lee, Won Woo Lee, You-Jin Jeon
The genus Dendronephthya encompasses marine soft corals that produce a wide spectrum of biofunctional terpenoids. Anticancer properties of these metabolites are widely exploited as potential chemotherapeutic agents. The present study reports the purification and isolation of a potential antiproliferative constituent, stigmast-5-en-3-ol from the 70% ethanol extract of the soft coral Dendronephthya gigantea. Among several other 3β-hydroxy-Δ5-steroidal congeners, stigmast-5-en-3-ol indicated prominent antiproliferative effects on HL-60 (leukemia) and MCF-7 (breast cancer) cell lines with IC50 values of 37...
July 13, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30012479/cytotoxicity-of-anticancer-candidate-salinomycin-and-identification-of-its-metabolites-in-rat-cell-cultures
#4
Lidia Radko, Małgorzata Olejnik
Salinomycin (SAL) is a polyether antibiotic, which is commonly used as a coccidiostat and has recently shown to exhibit anticancer activity. The toxic action of the drug may be connected with the extent and routes of its biotransformation. The cytotoxic potential of SAL and its combination with tiamulin and prednisolone was investigated using three cell models from rat: primary hepatocytes, hepatoma cells (FaO) and myoblasts (L6). The four biochemical endpoints were assessed: mitochondrial and lysosomal activity, total cell protein content and membrane integrity...
July 13, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30003980/delivery-efficiencies-of-constituents-of-combustion-derived-aerosols-across-the-air-liquid-interface-during-in-vitro-exposures
#5
Sandro Steiner, Pierrick Diana, Eric Dossin, Philippe Guy, Grégory Vuillaume, Athanasios Kondylis, Shoaib Majeed, Stefan Frentzel, Julia Hoeng
In vitro aerosol exposure of epithelial cells grown at the air-liquid interface is an experimental methodology widely used in respiratory toxicology. The exposure depends to a large part on the physicochemical properties of individual aerosol constituents, as they determine the transfer kinetics from the aerosol into the cells. We characterized the transfer of 70 cigarette smoke constituents from the smoke into aqueous samples exposed in the Vitrocell® 24/48 aerosol exposure system. The amounts of these compounds in the applied smoke were determined by trapping whole smoke in N,N-Dimethylformamide and then compared with their amounts in smoke-exposed, phosphate-buffered saline, yielding compound specific delivery efficiencies...
July 9, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30003979/verapamil-potentiates-anti-glioblastoma-efficacy-of-temozolomide-by-modulating-apoptotic-signaling
#6
Farina Hanif, Kahkashan Perveen, Saima M Malhi, Huma Jawed, Shabana U Simjee
Glioblastoma Multiforme (GBM) is the most malignant and invasive tumor of the CNS. Although temozolomide (TMZ) has improved the survival, long-lasting responses have not been reported. Therefore, there is a need to develop improved treatments, one of which might be newly identified drugs which can be used in combination therapy with low doses of standard drugs. Verapamil (VP) a known antihypertensive drug has been shown to enhance the activity of bis-chloroethylnitrosourea (BCNU), a drug used to treat GBM. Since, TMZ has replaced BCNU as the standard GBM chemotherapy; therefore, we aimed to study in vitro interaction of VP and TMZ against GBM...
July 9, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29966682/caspase-independence-and-characterization-of-bisnaphthalimidopropyl-spermidine-induced-cytotoxicity-in-hl60-cells
#7
Charles S Bestwick, Lesley Milne, Anne-Marie Dance, Gaela Cochennec, Gillian Cruickshank, Eflamm Allain, Lynda Constable, Susan J Duthie, Paul Kong Thoo Lin
Bisnaphthalimides are DNA intercalators of potential use as chemotherapeutics but for which the range of mechanism of action is only gradually being elucidated. Using human promyelocytic HL-60 cells, we extend characterization of the cytotoxicity of bisnaphthalimidopropylspermidine (BNIPSpd) and examine the relationship with caspase-activity. Within 4 h exposure, BNIPSpd (1-10 μM) induced significant DNA strand breakage. Evidence of apoptosis was progressive through the experimental period. Within 6 h, BNIPSpd increased the proportion of cells exhibiting plasma membrane phosphatidylserine exposure...
June 29, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29964147/acrolein-induces-ribotoxic-stress-in-human-cancer-cells-regardless-of-p53-status
#8
Chun-Hao Haung, Yen-Ting Chen, Jing-Heng Lin, Hsiang-Tsui Wang
Acrolein (Acr) cytotoxicity contributes to chemotherapeutic activity of cyclophosphamide via metabolism of the anticancer drug. Our previous studies have shown that Acr causes ribosomal DNA (rDNA) damages, thus shuts down ribosomal RNA (rRNA) synthesis and leads to ribosomal stress in human cancer cells. Ribosome senses stress in 28S rRNA and induces subsequent activation of mitogen-activated protein kinase (MAPK) pathway which triggers ribotoxic stress response (RSR). Here, we report that cells harboring p53 or not responds differently to Acr-induced RSR...
June 28, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29959992/oleocanthal-and-oleacein-contribute-to-the-in-vitro-therapeutic-potential-of-extra-virgin-oil-derived-extracts-in-non-melanoma-skin-cancer
#9
Beatrice Polini, Maria Digiacomo, Sara Carpi, Simone Bertini, Francesca Gado, Giuseppe Saccomanni, Marco Macchia, Paola Nieri, Clementina Manera, Stefano Fogli
Although the anticancer properties of extra virgin olive oil (EVOO) extracts have been recognized, the role of single compounds in non-melanoma skin cancer is still unknown. The in vitro chemopreventive and anticancer action of EVOO extracts and oil-derived compounds in non-melanoma skin cancer models were evaluated on cutaneous squamous cell carcinoma cells and on immortalized human keratinocytes stimulated with epidermal growth factor. Preparation of EVOO extracts and isolation of single compounds was carried out by chromatographic methods...
June 27, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29940344/an-investigation-into-e-cigarette-cytotoxicity-in-vitro-using-a-novel-3d-differentiated-co-culture-model-of-human-airways
#10
Pranav Vasanthi Bathrinarayanan, James E P Brown, Lindsay J Marshall, Laura J Leslie
Currently there is a lack of consensus on the possible adverse health effects of E-cigarettes (ECs). Important factors including cell model employed and exposure method determine the physiological relevance of EC studies. The present study aimed to evaluate EC cytotoxicity using a physiologically relevant in-vitro multicellular model of human airways. Human bronchial epithelial cells (CALU-3) and pulmonary fibroblasts (MRC-5) were co-cultured at air-liquid-interface for 11-14 days post which they were exposed to whole cigarette smoke (WCS) or EC vapour (ECV) at standard ISO-3308 regime for 7 m using a bespoke aerosol delivery system...
June 22, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29940343/comparison-of-different-cytotoxicity-assays-for-in-vitro-evaluation-of-mesoporous-silica-nanoparticles
#11
Katharina Braun, Christina Martina Stürzel, Johannes Biskupek, Ute Kaiser, Frank Kirchhoff, Mika Lindén
Colorimetric or luminogenic cytotoxicity assays are typically applied for in vitro cytotoxicity evaluations due to their easy handling and low cost. However, the results may be strongly assay-dependent. Furthermore, when applied to nanoparticle toxicity screening, nanoparticle-specific interferences can occur. Therefore, it is important to evaluate the assays for different classes of nanoparticles. Mesoporous silica nanoparticles (MSNs) have emerged as a promising platform for both diagnostic and therapeutic applications but a comparison between the commonly employed colorimetric formazan-dependent MTT and WST-1 and luminescent ATP-dependent cytotoxicity assays is still missing...
June 22, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29933105/stable-cellular-models-of-nuclear-receptor-pxr-for-high-throughput-evaluation-of-small-molecules
#12
Seema Negi, Shashi Kala Singh, Sanjay Kumar, Subodh Kumar, Rakesh K Tyagi
Pregnane & Xenobiotic Receptor (PXR) is one of the 48 members of the ligand-modulated transcription factors belonging to nuclear receptor superfamily. Though PXR is now well-established as a 'xenosensor', regulating the central detoxification and drug metabolizing machinery, it has also emerged as a key player in several metabolic disorders. This makes PXR attractive to both, researchers and pharmaceutical industry since clinical success of small drug molecules can be pre-evaluated on PXR platform. At the early stages of drug discovery, cell-based assays are used for high-throughput screening of small molecules...
June 19, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29933104/assessment-of-cytotoxicity-and-mutagenicity-of-exfoliated-graphene
#13
Katsuhide Fujita, Seiji Take, Ryotaro Tani, Junko Maru, Sawae Obara, Shigehisa Endoh
Graphene and related materials (GRMs) have unique optical and thermal characteristics and are expected to be adopted for industrial applications. However, there are concerns with respect to their safety to human health. To conduct cytotoxicity and mutagenicity assessments, exfoliated graphene (EGr) dispersed in Tween-20® was diluted in cell culture medium. Rat alveolar macrophage viability significantly decreased after 24 h exposure to 1 and 10 μg/mL EGr. No significant levels of intracellular reactive oxygen species were detected in the 2',7'-dichlorodihydrofluorescin diacetate assay after 24 h of exposure to EGr...
June 19, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29933103/human-oatp1b1-slco1b1-transports-sulfated-bile-acids-and-bile-salts-with-particular-efficiency
#14
Beáta Tóth, Márton Jani, Erzsébet Beéry, Teresa Heslop, Mark Bayliss, Neil R Kitteringham, B Kevin Park, Richard J Weaver, Peter Krajcsi
Human OATP1B1 is highly expressed at the basolateral membrane of the hepatocyte. It plays an important role in the sodium-independent transport of bile acids and bile salts and contributes to the systemic clearance of many drugs. In this study, the interaction of at least one representative of all major chemical classes of bile acids and bile salts, which include the bile acid chenodeoxycholate (CDC), monovalent (amidated) bile salts glycochenodeoxycholate (GCDC), taurochenodeoxycholate (TCDC) and taurocholate (TC), a sulfated bile acid 3-sulfo-chenodeoxycholate (3S-CDC) and a divalent (amidated and sulfated) bile salt 3-sulfo-glycolithocholate (3S-GLC) were tested with OATP1B1 overexpressed in HEK293 cells...
June 19, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29928970/gallic-acid-coated-sliver-nanoparticle-alters-the-expression-of-radiation-induced-epithelial-mesenchymal-transition-in-non-small-lung-cancer-cells
#15
S N Sunil Gowda, S Rajasowmiya, V Vadivel, S Banu Devi, A Celestin Jerald, S Marimuthu, N Devipriya
BACKGROUND: Radiotherapy is the most widely used treatment method for treating cancer with or without surgery and chemotherapy. In lung cancer, it is one of the important treatment steps in excising the tumor from the lung tissue; unfortunately, radiation can induce epithelial- mesenchymal transition (EMT), a typical physiological process in which cuboidal shaped epithelial cell loses its phenotype and acquires mesenchymal-like phenotype thus, increases the metastasis progression in the body...
June 18, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29928969/long-term-exposure-to-environmental-concentrations-of-diesel-exhaust-particles-does-not-impact-the-phenotype-of-human-bronchial-epithelial-cells
#16
Camille C Savary, Nessrine Bellamri, Claudie Morzadec, Sophie Langouët, Valérie Lecureur, Laurent Vernhet
Chronic exposure to diesel engine exhausts is associated with an increased risk of pulmonary diseases including lung cancer. Diesel engine exhausts contain large amounts of diesel exhaust particles (DEP) on which are adsorbed several carcinogenic compounds such as polycyclic aromatic hydrocarbons. Acute toxicity of high concentrations of DEP has been largely demonstrated in various in vitro cellular models. In contrast, the cellular and molecular impacts of low environmental concentrations of DEP on the phenotype of chronically exposed lung epithelial cells remain to be investigated...
June 18, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29913208/influence-of-parp-1-inhibition-in-the-cardiotoxicity-of-the-topoisomerase-2-inhibitors-doxorubicin-and-mitoxantrone
#17
Roberto Marques Damiani, Dinara Jaqueline Moura, Cassiana Macagnan Viau, Verônica Brito, Ana Moira Morás, João Antonio Pêgas Henriques, Jenifer Saffi
Doxorubicin (DOX) and Mitoxantrone (MTX) are very effective drugs for a range of tumors despite being highly cardiotoxic. DNA topoisomerase 2 beta (Top2ß) was revealed as key mediator of DOX-induced cardiotoxicity, although ROS generation is also an important mechanism. Oxidative stress is also an important issue in MTX-induced cardiotoxicity that is manifested by mitochondrial dysfunction. Studies have demonstrated the relationship between PARP-1 overactivation and cell viability in DOX-treated cardiomyocytes...
June 15, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29908305/in-vitro-toxicity-and-in-silico-docking-analysis-of-two-novel-selective-ah-receptor-modulators
#18
Selma Mahiout, Sara Giani Tagliabue, Atefeh Nasri, Iyekhoetin Matthew Omoruyi, Lars Pettersson, Laura Bonati, Raimo Pohjanvirta
The mediator of dioxin toxicity, aryl hydrocarbon receptor (AHR), has also important physiological functions. Selective AHR modulators (SAHRMs) share some effects of dioxins, except for their marked toxicity. We recently characterised toxicologically two novel SAHRMs, prodrugs IMA-08401 and IMA-07101 in rats, demonstrating that they are far less deleterious than the most toxic AHR-agonist, TCDD. Here, we analysed the in vitro toxicity and in silico AHR binding of the respective active, deacetylated metabolites, IMA-06201 (N-ethyl-N-phenyl-5-chloro-1,2-dihydro-4-hydroxy-1-methyl-2-oxo-quinoline-3-carboxamide) and IMA-06504 (N-(4-trifluoromethylphenyl)-1,2-dihydro-4-hydroxy-5-methoxy-1-methyl-2-oxo-quinoline-3-carboxamide)...
June 13, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29908304/pathway-based-predictive-approaches-for-non-animal-assessment-of-acute-inhalation-toxicity
#19
REVIEW
Amy J Clippinger, David Allen, Holger Behrsing, Kelly A BéruBé, Michael B Bolger, Warren Casey, Michael DeLorme, Marianna Gaça, Sean C Gehen, Kyle Glover, Patrick Hayden, Paul Hinderliter, Jon A Hotchkiss, Anita Iskandar, Brian Keyser, Karsta Luettich, Lan Ma-Hock, Anna G Maione, Patrudu Makena, Jodie Melbourne, Lawrence Milchak, Sheung P Ng, Alicia Paini, Kathryn Page, Grace Patlewicz, Pilar Prieto, Hans Raabe, Emily N Reinke, Clive Roper, Jane Rose, Monita Sharma, Wayne Spoo, Peter S Thorne, Daniel M Wilson, Annie M Jarabek
New approaches are needed to assess the effects of inhaled substances on human health. These approaches will be based on mechanisms of toxicity, an understanding of dosimetry, and the use of in silico modeling and in vitro test methods. In order to accelerate wider implementation of such approaches, development of adverse outcome pathways (AOPs) can help identify and address gaps in our understanding of relevant parameters for model input and mechanisms, and optimize non-animal approaches that can be used to investigate key events of toxicity...
June 13, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29906516/single-walled-carbon-nanotubes-affect-the-expression-of-genes-associated-with-immune-response-in-normal-human-astrocytes
#20
Oleksandr H Minchenko, Dariia O Tsymbal, Dmytro O Minchenko, Svitlana V Prylutska, Oksana S Hnatiuk, Yuriy I Prylutskyy, Nikos G Tsierkezos, Uwe Ritter
The effect of single-walled carbon nanotubes (SWCNTs) on the expression of a subset of immune response, apoptosis and cell proliferation -associated genes was studied in normal human astrocytes (line NHA/TS). In the cells treated with SWCNTs (2, 10 and 50 ng/ml of medium for 24 h) we observed a strong dose-dependent down-regulation of the expression of a cell surface glycoproteins HLA-DRA (major histocompatibility complex, class II, DR alpha) and HLA-DRB1. At the same time, the expression of HLA-F (major histocompatibility complex, class I, F), LMNB1 (lamin B1), and HTRA1 (high temperature requirement A1) genes as well as the level of miR-190b and miR-7 was up-regulated in NHA/TS subjected to different concentrations of SWCNTs...
June 12, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
journal
journal
29691
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"