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Toxicology in Vitro: An International Journal Published in Association with BIBRA

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https://www.readbyqxmd.com/read/28923420/high-doses-of-lipid-core-nanocapsules-do-not-affect-bovine-embryonic-development-in-vitro
#1
Caroline Lucas, Mariana Remião, Franciele Bruinsmann, Isadora Lopes, Morgana Borges, Ana Laura Feijó, Andrea Cristina Basso, Adriana Pohlmann, Silvia Guterres, Vinicius Campos, Fabiana Seixas, Tiago Collares
The improvement of in vitro embryo production by culture media supplementation has been a potential tool to increase blastocyst quality and development. Recently, lipid-core nanocapsules (LNC), which were developed for biomedical applications as a drug-delivery system, have demonstrated beneficial effects on in vitro embryo production studies. LNCs have a core composed of sorbitan monostearate dispersed in capric/caprylic triglyceride. Based on that, we firstly investigated if LNCs supplemented during in vitro oocyte maturation had affinity to the mineral oil placed over the top of the IVM media...
September 15, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28919358/perfused-human-hepatocyte-microtissues-identify-reactive-metabolite-forming-and-mitochondria-perturbing-hepatotoxins
#2
Cliff Rowe, Mohsen Shaeri, Emma Large, Terri Cornforth, Angela Robinson, Tomasz Kostrzewski, Rowena Sison-Young, Christopher Goldring, Kevin Park, David Hughes
Hepatotoxins cause liver damage via many mechanisms but the formation of reactive metabolites and/or damage to liver mitochondria are commonly implicated. We assess 3D human primary hepatocyte microtissues as a platform for hepatotoxicity studies with reactive metabolite-forming and mitochondria-perturbing compounds. We show that microtissues formed from cryopreserved human hepatocytes had bile canaliculi, transcribed mRNA from genes associated with xenobiotic metabolism and expressed functional cytochrome P450 enzymes...
September 15, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28919359/human-intestine-and-liver-microsomal-metabolic-differences-between-c19-diester-and-monoester-diterpenoid-alkaloids-from-the-roots-of-aconitum-carmichaelii-debx
#3
Min Zhang, Chong-Sheng Peng, Xiao-Bo Li
The roots of Aconitum carmichaelii Debx. show excellent effects against rheumatism and cardiovascular diseases, but the effective compounds, C19-diester and monoester diterpenoid alkaloids (DDAs and MDAs) are toxic for their narrow therapeutic windows. It is noteworthy to investigate intestinal metabolism of these toxic compounds mainly by oral administration, because gut also express drug-metabolizing enzymes as well as liver. This study initially focused on phase I and phase II metabolism of DDAs (including aconitine, mesaconitine and hypaconitine) and MDAs (including benzoylaconine, benzoylmesaconine and benzoylhypaconine) in human intestine microsomes (HIM), with comparison of metabolism in human liver microsomes (HLM)...
September 14, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28911986/virtual-cell-based-assay-simulation-of-intra-mitochondrial-concentration-in-hepatocytes-and-cardiomyocytes
#4
Andrew P Worth, Jochem Louisse, Peter Macko, Jose Vicente Sala Benito, Alicia Paini
In order to replace the use of animals in toxicity testing, there is a need to predict human in vivo toxic doses from concentrations that cause adverse effects in in vitro test systems. The virtual cell based assay (VCBA) has been developed to simulate intracellular concentrations as a function of time, and can be used to interpret in vitro concentration-response curves. In this study we refine and extend the VCBA model by including additional target-organ cell models and by simulating the fate and effects of chemicals at the organelle level...
September 11, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28911985/moving-forward-in-carcinogenicity-assessment-report-of-an-eurl-ecvam-estiv-workshop
#5
Raffaella Corvi, Federica Madia, Kathryn Z Guyton, Peter Kasper, Ruthann Rudel, Annamaria Colacci, Jos Kleinjans, Paul Jennings
There is an increased need to develop novel alternative approaches to the two-year rodent bioassay for the carcinogenicity assessment of substances where the rodent bioassay is still a basic requirement, as well as for those substances where animal use is banned or limited or where information gaps are identified within legislation. The current progress in this area was addressed in a EURL ECVAM- ESTIV workshop held in October 2016, in Juan les Pins. A number of initiatives were presented and discussed, including data-driven, technology-driven and pathway-driven approaches...
September 11, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28899767/effects-of-hexavalent-chromium-on-mouse-splenic-t-lymphocytes
#6
Lu Dai, Wenhua Xu, Hui Li, Jacqueline A Frank, Caigu He, Zhuo Zhang, Gang Chen
Hexavalent chromium [Cr(VI)] is widely used in various industrial processes and has been recognized as a carcinogen. As the first line of host defense system, the immune system can be a primary target of Cr(VI). T cell population represents a major arm of the immune system that plays a critical role in host anti-tumor immunity. Dysfunction of T cells, such as exhaustion under the persistent presence of tumor antigen, compromise host anti-tumor immunity resulting in oncogenesis. Previous studies have shown Cr(VI) exposure alters the phenotype of human peripheral blood lymphocytes...
September 9, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28893613/genotoxic-effects-in-transformed-and-non-transformed-human-breast-cell-lines-after-exposure-to-silver-nanoparticles-in-combination-with-aluminium-chloride-butylparaben-or-di-n-butylphthalate
#7
J Roszak, K Domeradzka-Gajda, A Smok-Pieniążek, A Kozajda, S Spryszyńska, J Grobelny, E Tomaszewska, K Ranoszek-Soliwoda, M Cieślak, D Puchowicz, M Stępnik
In the present study genotoxic effects after combined exposure of human breast cell lines (MCF-10A, MCF-7 and MDB-MB-231) to silver nanoparticles (AgNP, citrate stabilized, 15 and 45nm by STEM, Ag15 and Ag45, respectively) with aluminium chloride, butylparaben, or di-n-butylphthalate were studied. In MCF-10A cells exposed for 24h to Ag15 at the concentration of 23.5μg/mL a statistically significant increase in DNA damage in comet assay (SSB) was observed. In the presence of the test chemicals the genotoxic effect was decreased to a level comparable to control values...
September 8, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28888659/development-of-a-luminescent-mutagenicity-test-for-high-throughput-screening-of-aquatic-samples
#8
Nick Zwart, Marja Lamoree, Corine Houtman, Jacob de Boer, Jeroen Kool, Timo Hamers
The Salmonella reversion based Ames test is the most widely used method for mutagenicity testing. For rapid toxicity assessment of e.g. water samples and for effect-directed analysis, however, the Ames test suffers from lack of throughput and is regarded as a laborious, time consuming method. To achieve faster analysis, with increased throughput, a (downscaled) luminescent derivative of the Ames Salmonella/microsome fluctuation test has been developed through expression of the Photorhabdus luminescens luciferase in the Salmonella TA98 and TA100 strains...
September 6, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28887287/characterization-of-simvastatin-acid-uptake-by-organic-anion-transporting-polypeptide-3a1-oatp3a1-and-influence-of-drug-drug-interaction
#9
Amandla Atilano-Roque, Melanie S Joy
Human organic anion transporting polypeptide 3A1 (OATP3A1) is predominately expressed in the heart. The ability of OATP3A1 to transport statins into cardiomyocytes is unknown, although other OATPs are known to mediate the uptake of statin drugs in liver. The pleiotropic effects and uptake of simvastatin acid were analyzed in primary human cardiomyocytes and HEK293 cells transfected with the OATP3A1 gene. Treatment with simvastatin acid reduced indoxyl sulfate-mediated reactive oxygen species and modulated OATP3A1 expression in cardiomyocytes and HEK293 cells transfected with the OATP3A1 gene...
September 6, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28887288/development-of-a-quantitative-cytotoxicity-assay-using-mouse-lymphoma-tk-cells
#10
Xuemei Liu, Fan Zhang, Denise Rodeheaver, Ann Wright, Charlon Tolliver, Jamie Walker, Robert Rose
We describe a new, quantitative, cytotoxicity method using mouse lymphoma L5178Y thymidine kinase+/- (TK) suspension cells for medical device safety assessment that has equivalent sensitivity, and greater efficiency compared to the colony formation assay (CFA) using adherent V79-4 cells. Assays were carried out in 24-well plates. TK cells were seeded on concave side of six types of contact lenses and cell growth rate after 72h determined using the Vi-Cell XR. Five of six contact lenses studied demonstrated the same results between the TK and the CFA methods...
September 5, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28886972/benzene-metabolite-hydroquinone-induces-apoptosis-of-bone-marrow-mononuclear-cells-through-inhibition-of-%C3%AE-catenin-signaling
#11
Jiaqi Li, Songfu Jiang, Yi Chen, Ruye Ma, Jingjing Chen, Shanhu Qian, Yifen Shi, Yixiang Han, Shenghui Zhang, Kang Yu
The Akt/glycogen synthase kinase-3β (GSK-3β)/β-catenin signaling pathway has been shown to play an important role in hematopoiesis, and hematopoietic cells are sensitive targets for benzene-induced hematotoxicity. We therefore hypothesized that dysregulation of the Akt/GSK-3β/β-catenin signaling was associated with benzene-induced hematotoxicity. Here, we showed that hydroquinone (HQ), a major metabolite of benzene in humans, significantly inhibited cell viability and colony formation while inducing apoptosis of human bone marrow mononuclear cells in vitro...
September 5, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28882705/prediction-of-skin-sensitization-potency-sub-categories-using-peptide-reactivity-data
#12
Britta Wareing, Daniel Urbisch, Susanne Noreen Kolle, Naveed Honarvar, Ursula G Sauer, Annette Mehling, Robert Landsiedel
While the skin sensitization hazard of substances can already be identified using non-animal methods, the classification of potency sub-categories GHS-1A and 1B is still challenging. Potency can be measured by the dose at which an effect is observed; since the protein-adduct formation is determining the dose of the allergen in the skin, peptide reactivity was used to assess the potency. The Direct Peptide Reactivity Assay (DPRA; one concentration and reaction-time) did not sufficiently discriminate between sub-categories 1A and 1B (56% accuracy compared to LLNA data, n=124)...
September 4, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28882704/bioluminescent-enzyme-inhibition-based-assay-to-predict-the-potential-toxicity-of-carbon-nanomaterials
#13
Elena N Esimbekova, Elena V Nemtseva, Anna E Bezrukikh, Galina V Jukova, Albert E Lisitsa, Viktoriya I Lonshakova-Mukina, Nadezhda V Rimatskaya, Oleg S Sutormin, Valentina A Kratasyuk
A bioluminescent enzyme inhibition-based assay was applied to predict the potential toxicity of carbon nanomaterials (CNM) presented by single- and multi-walled nanotubes (SWCNT and MWCNT) and aqueous solutions of hydrated fullerene С60 (C60HyFn). This assay specifically detects the influence of substances on parameters of the soluble or immobilised coupled enzyme system of luminescent bacteria: NAD(P)Н:FMN-oxidoreductase+luciferase (Red+Luc). A protocol based on the optical properties of CNM for correcting the results of the bioluminescent assay was also developed...
September 4, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28870540/con4ei-slug-mucosal-irritation-smi-test-method-for-hazard-identification-and-labelling-of-serious-eye-damaging-and-eye-irritating-chemicals
#14
E Adriaens, R Guest, J A Willoughby, P Fochtman, H Kandarova, S Verstraelen, A R Van Rompay
Assessment of ocular irritancy is an international regulatory requirement in the safety evaluation of industrial and consumer products. Although many in vitro ocular irritation assays exist, alone they are incapable of fully categorizing chemicals. The objective of CEFIC-LRI-AIMT6-VITO CON4EI (CONsortium for in vitro Eye Irritation testing strategy) project was to develop tiered testing strategies for eye irritation assessment that can lead to complete replacement of the in vivo Draize rabbit eye test (OECD TG 405)...
September 1, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28867505/dose-response-in-a-high-density-three-dimensional-liver-device-with-real-time-bioenergetic-and-metabolic-flux-quantification
#15
Andrey P Tikunov, Yoo-Sik Shim, Narayan Bhattarai, Scott Q Siler, Valerie Soldatow, Edward L LeCluyse, Jonathan E McDunn, Paul B Watkins, Jeffrey M Macdonald
Real-time dose-response curves for fructose have been non-invasively determined in primary rat hepatocyte alginate spheroids cultured in a NMR-compatible fluidized-bed bioreactor. Using (13)C-labeled glucose and glycine culture medium, fructose dose was compared to glucose uptake and glycogen synthesis rate using (13)C NMR spectroscopy, and to ATP and fructose-1-phosphate concentration using (31)P NMR spectroscopy. A highly efficient multicoaxial perfusion system maintains high density 3-D hepatocyte cultures, permitting (13)C and (31)P NMR spectral time courses with 1min time points...
September 1, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28866024/con4ei-consortium-for-in-vitro-eye-irritation-testing-strategy-epiocular%C3%A2-time-to-toxicity-epiocular-et-50-protocols-for-hazard-identification-and-labelling-of-eye-irritating-chemicals
#16
Helena Kandarova, Silvia Letasiova, Els Adriaens, Robert Guest, Jamin A Willoughby, Agnieszka Drzewiecka, Katarzyna Gruszka, Nathalie Alépée, Sandra Verstraelen, An R Van Rompay
Assessment of acute eye irritation potential is part of the international regulatory requirements for testing of chemicals. The objective of the CON4EI (CONsortium for in vitro Eye Irritation testing strategy) project was to develop tiered testing strategies for eye irritation assessment for all drivers of classification. A set of 80 reference chemicals (38 liquids and 42 solids) was tested with eight different alternative methods. Here, the results obtained with reconstructed human cornea-like epithelium (RhCE) EpiOcular™ in the EpiOcular time-to-toxicity Tests (Neat and Dilution ET-50 protocols) are presented...
August 30, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28855101/in-vitro-observations-and-in-silico-predictions-of-xenoestrogen-mixture-effects-in-t47d-based-receptor-transactivation-and-proliferation-assays
#17
Nina Schlotz, Gwang-Jin Kim, Stefan Jäger, Stefan Günther, Evelyn Lamy
Within endocrine disruptor research, evaluation and interpretation of mixture effects and the predictive value for downstream responses still warrant more in-depth investigations. We used an estrogen receptor (ER)-mediated reporter gene assay (ER-CALUX®) and a cell proliferation assay (WST-1 assay), both based on the T47D breast cancer cell line, to test mixtures of heterogeneous xenoestrogens. Observed concentration-response curves were compared to those predicted by the concepts of concentration addition (CA), generalized concentration addition (GCA), and a novel full logistic model (FLM)...
August 30, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28844819/in-vitro-studies-on-the-tumorigenic-potential-of-the-halonitromethanes-trichloronitromethane-and-bromonitromethane
#18
Alicia Marsà, Constanza Cortés, Elisabet Teixidó, Alba Hernández, Ricard Marcos
Epidemiological data indicate that chronic exposure to water disinfection by-products (DBPs) may result in increased risk of cancer. However, the real carcinogenic potential of individual DBPs is not well known. In this study, we assessed the in vitro carcinogenic potential of trichloronitromethane (TCNM) and bromonitromethane (BNM), two halonitromethanes (HNMs) commonly found in DBPs' mixtures at comparably high concentrations. Human lung BEAS-2B cells were exposed for 8weeks to TCNM and BNM, and the acquisition of different in vitro cancer-like features was evaluated...
August 24, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28843493/utilization-of-ipsc-derived-human-neurons-for-high-throughput-drug-induced-peripheral-neuropathy-screening
#19
Payal Rana, Gregory Luerman, Dietmar Hess, Elizabeth Rubitski, Karissa Adkins, Christopher Somps
As the number of cancer survivors continues to grow, awareness of long-term toxicities and impact on quality of life after chemotherapy treatment in cancer survivors has intensified. Chemotherapy-induced peripheral neuropathy (CIPN) is one of the most common side effects of modern chemotherapy. Animal models are used to study peripheral neuropathy and predict human risk; however, such models are labor-intensive and limited translatability between species has become a major challenge. Moreover, the mechanisms underlying CIPN have not been precisely determined and few human neuronal models to study CIPN exist...
August 24, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28843492/effects-of-gasoline-and-ethanol-gasoline-exhaust-exposure-on-human-bronchial-epithelial-and-natural-killer-cells-in-vitro
#20
Michèle Roth, Jakob Usemann, Christoph Bisig, Pierre Comte, Jan Czerwinski, Andreas C R Mayer, Konstantin Beier, Barbara Rothen-Rutishauser, Philipp Latzin, Loretta Müller
Air pollution exposure, including passenger car emissions, may cause substantial respiratory health effects and cancer death. In western countries, the majority of passenger cars are driven by gasoline fuel. Recently, new motor technologies and ethanol fuels have been introduced to the market, but potential health effects have not been thoroughly investigated. We developed and verified a coculture model composed of bronchial epithelial cells (ECs) and natural killer cells (NKs) mimicking the human airways to compare toxic effects between pure gasoline (E0) and ethanol-gasoline-blend (E85, 85% ethanol, 15% gasoline) exhaust emitted from a flexfuel gasoline car...
August 24, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
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