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Toxicology in Vitro: An International Journal Published in Association with BIBRA

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https://www.readbyqxmd.com/read/30243732/leveraging-proteomics-to-compare-submerged-versus-air-liquid-interface-carbon-nanotube-exposure-to-a-3d-lung-cell-model
#1
G Hilton, H Barosova, A Petri-Fink, B Rothen-Rutishauser, M Bereman
With the emerging concern over the potential toxicity associated with carbon nanotube inhalation exposure, several in vitro methods have been developed to evaluate cellular responses. Since the major concern for adverse effects by carbon nanotubes is inhalation, various lung cell culture models have been established for toxicity testing, thus creating a wide variation of methodology. Limited studies have conducted side-by-side comparisons of common methods used for carbon nanotube hazard testing. The aim of this work was to use proteomics to evaluate global cellular response, including pro-inflammatory and pro-fibrotic mediators, of a 3D lung model composed of macrophages, epithelial cells, and fibroblasts which mimics the human alveolar epithelial tissue barrier...
September 19, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30243731/butyl-benzyl-phthalate-promotes-prostate-cancer-cell-proliferation-through-mir-34a-downregulation
#2
Mingming Zhu, Jieshu Wu, Xiao Ma, Cong Huang, Rui Wu, Weiwei Zhu, Xiaoting Li, Zhaofeng Liang, Feifei Deng, Jianyun Zhu, Wei Xie, Xue Yang, Ye Jiang, Shijia Wang, Shanshan Geng, Chunfeng Xie, Caiyun Zhong
Prostate cancer is the most common malignancy in men. Phthalate esters are a class of environmental endocrine disruptors and were reported to be cancer promoting agents, however the potential role of phthalate esters in prostate cancer has been rarely reported. Mounting evidence has shown that miR-34a is a master tumor suppressor miRNA in cancer. The aim of this study was to investigate the role of butyl benzyl phthalate (BBP), one of the typical phthalate esters, in cell proliferation of prostate cancer cells...
September 19, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30243730/the-organophosphorus-pesticide-dimethoate-decreases-cell-viability-and-induces-changes-in-different-biochemical-parameters-of-rat-pancreatic-stellate-cells
#3
Salomé Martínez-Morcillo, Marcos Pérez-López, Francisco Soler-Rodríguez, Antonio González
In the present study we employed cultured pancreatic stellate cells to study the effect of the organophosphorus insecticide dimethoate on pancreatic cell physiology. Esterase activity, cell viability, reactive oxygen species generation and Ca2+ mobilization were examined. Our results show that dimethoate (0.1, 1 and 10 μM) induced a concentration-dependent inhibition of cholinesterase enzymatic activity at all concentrations tested. A drop in carboxylesterase activity was noted in the presence of 10 μM dimethoate...
September 19, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30240709/interactions-between-oxidative-stress-autophagy-and-apoptosis-in-a549-cells-treated-with-aged-black-carbon
#4
Jing An, Qian Zhou, Meiying Wu, Lu Wang, Yufang Zhong, Jialiang Feng, Yu Shang, Yingjun Chen
After emitted from incomplete combustion of fossil fuels and biomass, ambient black carbon (BC) was then undergone photochemical oxidization processes in the air to form aged BC particles, also called oxidized BC (OBC). This study aimed to investigate the interactions between oxidative stress, autophagy and apoptosis induced by OBC in A549 cells and to explore associated molecular mechanisms. First, OBC could stimulate oxidative stress, autophagy and apoptosis dose-dependently, as evidenced by increased intercellular reactive oxygen species (ROS) levels, up-regulated autophagosome markers (light chain 3, LC3), and elevated apoptosis rate...
September 18, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30240708/a-comparative-study-of-skin-irritation-caused-by-novel-bis-quaternary-ammonium-compounds-and-commonly-used-antiseptics-by-using-cell-culture-methods
#5
Masashi Yamamoto, Reiko Matsumura, Yoshihiko Hirata, Hideaki Nagamune
New bis-quaternary ammonium compounds (bis-QACs) 3-(3-hydroxy-2-(hydroxymethyl)-2-{[(1-dodecylpyridinium-3-yl)oxy]methyl}propoxy)-1-dodecylpyridinium dibromide (3HHDMP-12) and 3,3'-[1,4-phenylenebis(oxy)]bis(1-dodecylpyridinium) dibromide (3PHBO-12) were compared with commonly-used antiseptics such as benzalkonium chloride (BAC), octenidine dihydrochloride (OCT), chlorhexidine digluconate (CHG) and polyhexamethylene biguanide (PHMB), to evaluate their potential to cause skin irritation. The cytotoxicity of these compounds in various cultured cells, as well as their effect on the expression of inflammatory cytokine genes such as IL-1α were evaluated...
September 18, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30218698/assessing-bioactivity-exposure-profiles-of-fruit-and-vegetable-extracts-in-the-biomap-profiling-system
#6
Barbara A Wetmore, Rebecca A Clewell, Brian Cholewa, Bethany Parks, Salil N Pendse, Michael B Black, Kamel Mansouri, Saad Haider, Ellen L Berg, Richard S Judson, Keith A Houck, Matthew Martin, Harvey J Clewell, Melvin E Andersen, Russell S Thomas, Patrick D McMullen
The ToxCast program has generated in vitro screening data on over a thousand chemicals to assess potential disruption of important biological processes and assist in hazard identification and chemical testing prioritization. Few results have been reported for complex mixtures. To extend these ToxCast efforts to mixtures, we tested extracts from 30 organically grown fruits and vegetables in concentration-response in the BioMAP® assays. BioMAP systems use human primary cells primed with endogenous pathway activators to identify phenotypic perturbations related to proliferation, inflammation, immunomodulation, and tissue remodeling...
September 12, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30218697/di-n-butyl-phthalate-modifies-pma-induced-macrophage-differentiation-of-thp-1-monocytes-via-ppar%C3%AE
#7
Vegard Sæter Grytting, Bergitte Pearl Olderbø, Jørn A Holme, Jan Tore Samuelsen, Anita Solhaug, Rune Becher, Anette Kocbach Bølling
The present study examined effects of di-butyl phthalate (DBP) on phorbol myristate acetate (PMA)-induced macrophage differentiation of THP-1 monocytes, determined by morphological classification and flow cytometry. Focusing on expression of the surface marker CD36, the potential role of peroxisome proliferator-activated receptor gamma (PPARγ) was examined using various PPARγ agonists and antagonists. As the PPARγ ligand-binding domain contains multiple ligand-binding sites (LBS), agonist and antagonists targeting the different sites were used...
September 12, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30217652/mifepristone-potentiates-etoposide-toxicity-in-hep-g2-cells-by-modulating-drug-transport
#8
Z Dostál, P Kosina, P Mlejnek, K Kikalová, M Modrianský
Etoposide is a well-known and widely used anticancer drug that displays several side effects. In addition, tumors often acquire resistance to this drug. Our aim is to develop a combination therapy that would augment toxicity of etoposide in malignant cells. Based on literature and our experiments, we selected mifepristone (RU486) as a potential supporting molecule that is able to enhance etoposide toxicity against cancer cells. All experiments were performed with Hep G2 cells, a well-known and described human hepatocellular carcinoma cell line...
September 11, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30196099/integrated-analysis-of-in-vitro-data-and-the-adverse-outcome-pathway-framework-for-prioritization-and-regulatory-applications-an-exploratory-case-study-using-publicly-available-data-on-piperonyl-butoxide-and-liver-models
#9
Noffisat O Oki, Lucian Farcal, Ahmed Abdelaziz, Oana Florean, Tatyana Y Doktorova, Thomas Exner, Pekka Kohonen, Roland Grafström, Barry Hardy
The integration of existing knowledge to support the risk assessment of chemicals is an ongoing challenge for scientists, risk assessors and risk managers. In addition, European Union regulations limiting the use of new animal testing in cosmetics makes already existing information even more valuable. Applying a previous SEURAT-1 program framework to derive predictions of in vivo toxicity responses for a compound, we selected piperonyl butoxide (PBO) as a case study for identification of knowledge and methodology gaps in understanding a compound's effects on the human liver...
September 6, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30195042/antioxidant-and-pro-oxidant-mechanisms-of-catechin-in-microsomal-cyp2e1-dependent-oxidative-stress
#10
Andres A Caro, Alanna Davis, Sydney Fobare, Nicholas Horan, Cameron Ryan, Cara Schwab
The objectives of this work were to evaluate the effects of catechin on cytochrome P450 2E1 (CYP2E1)-dependent oxidative stress. Microsomes co-expressing human CYP2E1 with NADPH cytochrome P450 reductase and cytochrome b5 were incubated with NADPH and DTPA at pH 7.0. Superoxide anion generation was specifically detected by spin-trapping with DEPMPO. Generation of the DEPMPO-OOH adduct was not observed in the absence of CYP2E1 and in the presence of superoxide dismutase (SOD) or catechin, while catalase was ineffective...
September 6, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30195041/alpha-mangostin-an-active-compound-in-garcinia-mangostana-abrogates-anoikis-resistance-in-human-hepatocellular-carcinoma-cells
#11
Benjawan Wudtiwai, Pornsiri Pitchakarn, Ratana Banjerdpongchai
Anoikis-resistance is a critical step in cancer progression, especially during the process of metastasis. During this phase, the cancer phenotype that causes cell survival in detachment conditions, drug resistance, and epithelial-to-mesenchymal transition (EMT) is altered. Inhibition of anoikis-resistance can potentially be the molecular target in cancer therapy. Alpha-mangostin, an active compound in Garcinia mangostana, has been reported for its cell-death induction and its chemosensitizing and anti-metastatic properties in many cancer cell types, such as ovarian cancer, lung cancer, and hepatocellular carcinoma...
September 6, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30189235/inhibition-of-organic-cation-transporter-oct-activities-by-carcinogenic-heterocyclic-aromatic-amines
#12
Katia Sayyed, Christophe Camillerapp, Marc Le Vée, Arnaud Bruyère, Anne T Nies, Ziad Abdel-Razzak, Olivier Fardel
Carcinogenic heterocyclic aromatic amines (HAAs) interact with some drug transporters, like the efflux pump BCRP and the organic anion transporters OAT1 and OAT3. The present study was designed to determine whether they can also target activities of the organic cation transporters (OCTs), using mainly OCT1-, OCT2- and OCT3-overexpressing HEK293 cells. Fifteen HAAs were demonstrated to differently alter OCT activities; with a cut-off of at least 50% reduction of transporter activity by 100 μM HAAs, 5/15 HAAs, including Trp-P-1 and Trp-P-2, inhibited activities of OCT1, OCT2 and OCT3, whereas 7/15 HAAs, including PhIP and MeIQx, blocked those of OCT2 and OCT3, 1/15 HAAs reduced those of OCT1 and OCT2 and 2/15 HAAs, including AαC, only that of OCT2...
September 3, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30149078/human-skin-explants-an-in-vitro-approach-for-assessing-uvb-induced-damage
#13
Christian Khalil
Lifestyle changes involving frequent outdoor activities are contributing to higher exposure to harmful ultraviolet light (UVB). The acute effects of UVB irradiation on human skin was evaluated in this study using freshly excised human skin from elective surgery subjected to UVB doses (0-3.76 J/cm2 ). The assessment of UVB induced cellular and skin damages was undertaken at two time points immediately and 24 h post exposure using in vitro, and immunohistochemical staining techniques. The results indicated no significant loss of skin integrity or significant acute mitochondrial cellular damages in UVB exposed skin sections as measured by the MTS cytotoxicity assay...
August 24, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30149077/fumonisin-production-and-toxic-capacity-in-airborne-black-aspergilli
#14
Daniela Jakšić, Sándor Kocsubé, Ottó Bencsik, Anita Kecskeméti, András Szekeres, Dubravko Jelić, Nevenka Kopjar, Csaba Vágvölgyi, János Varga, Maja Šegvić Klarić
No abstract text is available yet for this article.
August 24, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30145358/n-acetylcysteine-prevents-cytotoxic-effects-induced-by-man-made-mineral-fibers-in-a-human-bronchial-epithelial-cell-line
#15
Min Zhang, HaiLing Xia, Min Yu, LiJin Zhu, Li Ju, JunQiang Chen, JiaJun Zhao, Yun Xiao, Kun Chen
Man-made mineral fibres (MMMFs) such as glass wool (GW), rock wool (RW) and refractory ceramic fibres (RCFs) are widely used as substitutes of asbestos. The present study aimed to investigate the cytotoxic effects on human bronchial epithelial cells (BEAS-2B) exposed to GW1, RW1 and RCF2, considering their properties similar to that of asbestos. We assessed cell viability; cell morphological changes; apoptotic rate; DNA damage; reactive oxygen species (ROS) generation; activities of caspase-3, caspase-8 and caspase-9; and expression levels of FasL, phosphorylated p38, and total p38 MAPK proteins...
August 23, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30144576/glucotoxicity-results-in-apoptosis-in-h9c2-cells-via-alteration-in-redox-homeostasis-linked-mitochondrial-dynamics-and-polyol-pathway-and-possible-reversal-with-cinnamic-acid
#16
Nair Anupama, M R Preetha Rani, G L Shyni, K G Raghu
Several mechanisms have been proposed for the heart dysfunction during hyperglycemia. The aim of the present in vitro study is to elucidate the role of alterations in redox homeostasis in the induction of apoptosis during hyperglycemia in H9c2 cells via dysfunction in mitochondria and polyol pathway and evaluation of the beneficial effect of cinnamic acid against the same. The H9c2 cells were incubated with 33 mM glucose for 48 h to simulate the diabetic condition. Cell injury was confirmed with a significant increase of atrial natriuretic peptide and lactate dehydrogenase release...
August 23, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30144575/involvement-of-pink1-parkin-mediated-mitophagy-in-paraquat-induced-apoptosis-in-human-lung-epithelial-like-a549-cells
#17
Da-Zhuang Sun, Chun-Qing Song, Yong-Min Xu, Rui Wang, Wei Liu, Zhi Liu, Xue-Song Dong
Paraquat (PQ) is one of the most popular herbicides and has been widely used all over the world over the past several decades. However, PQ exposure can cause multiple organ failure, especially acute lung injury in humans as well as in rodent animals. Mitochondrial dysfunction plays a crucial role in PQ-induced lung cell damage. Mitophagy, defined as the selective autophagic elimination process of mitochondria, is a significant mechanism controlling mitochondrial quality. In this study, we investigated PINK1/Parkin-mediated mitophagy activated in the process of the PQ-induced cell apoptosis by using human lung epithelial-like A549 cells...
August 23, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30144574/immunostimulatory-effect-of-zinc-phthalocyanine-derivatives-on-macrophages-based-on-the-pro-inflammatory-tnf%C3%AE-and-il1%C3%AE-cytokine-production-levels
#18
Furkan Ayaz, Abdulcelil Yuzer, Mine Ince
In this study, we examined the effect of halogen substitution on the ability of ZnPcs to stimulate or regulate the immune system cells. There have been studies focusing on the usage of Pcs as treatment option against different cancer types. More attention should be paid on their possible positive or negative effects on the immune system for better prognosis rates. We designed and synthesized unique ZnPcs with iodine substitution and further tested their effect on mammalian macrophage cell line. Macrophages are crucial cell types that can define the immune response by cytokine production as well as by antigen presentation to the other immune system cells after phagocytosis of the danger associated molecules...
August 23, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30138673/using-bisphenol-a-and-its-analogs-to-address-the-feasibility-and-usefulness-of-the-calux-ppar%C3%AE-assay-to-identify-chemicals-with-obesogenic-potential
#19
Camille Dusserre, Julie Mollergues, Elena Lo Piparo, Martin Smieško, Maricel Marin-Kuan, Benoit Schilter, Karma Fussell
Environmental chemical exposures have been implicated in the obesity epidemic as potential mis-regulators of a variety of metabolic pathways. As agonism of the peroxisome proliferator-activated nuclear hormone receptor γ (PPARγ) is one of the suspected mechanisms involved, a PPARγ screening assay may have relevance for the biodetection of such effects of environmental chemicals. To test this hypothesis, we established the PPARγ2 -CALUX® assay in-house and tested it against a number of known and suspected PPARγ modulators...
August 20, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/30134184/mim1-the-mcl-1-specific-bh3-mimetic-induces-apoptosis-in-human-u87mg-glioblastoma-cells
#20
Michalina Respondek, Artur Beberok, Jakub Rok, Zuzanna Rzepka, Dorota Wrześniok, Ewa Buszman
Glioblastoma multiforme (GBM) is the most common and high aggressive malignant brain tumor. Despite evolving oncology treatment and novel chemotherapeutic agents the median survival of patients diagnosed with GBM is only 12-15 months. This grim fact highlights necessity to identify new drugs that could improve the effectiveness of GBM patients treatment. MIM1 is a specific low molecular Mcl-1 protein inhibitor able to induce Mcl-1-dependent cancer cells death. The aim of this study was to examine the effect of MIM1 as well as MIM1 and temozolomide (TMZ) mixture on cell viability, apoptosis and cell cycle progression in human U87MG glioblastoma cells...
August 19, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
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