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Toxicology in Vitro: An International Journal Published in Association with BIBRA

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https://www.readbyqxmd.com/read/27888128/neutrophil-gelatinase-associated-lipocalin-production-negatively-correlates-with-hk-2-cell-impairment-evaluation-of-ngal-as-a-marker-of-toxicity-in-hk-2-cells
#1
Martina Hauschke, Erika Roušarová, Pavel Flídr, Jan Čapek, Antonín Libra, Tomáš Roušar
Neutrophil gelatinase-associated lipocalin is an extracellular protein produced mostly in kidney. Recently, it has become a promising biomarker of renal damage in vivo. On the other hand, the validation of NGAL as a biomarker for nephrotoxicity estimation in vitro has not been characterized in detail yet. Since the HK-2 cells are frequently used human kidney cell line, we aimed to characterize the production of NGAL in these cells and to evaluate NGAL as a possible marker of cell impairment. We used heavy metals (mercury, cadmium), peroxide, drugs (acetaminophen, gentamicin) and cisplatin to mimic nephrotoxicity...
November 22, 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27884723/ir-and-igf-1r-expression-affects-insulin-induced-proliferation-and-dna-damage
#2
Eman Maher Othman, Tahanee Altabaa, Henning Hintzsche, Helga Stopper
Diabetes mellitus type 2 is in its prediagnostic and early phase characterized by hyperinsulinemia. Previously, we pointed out hyperinsulinemia as a potential link between diabetes mellitus and the increased cancer risk that is associated with this disease through its induction of oxidative stress and DNA damage. In the present study, we address the relationship between the induction of proliferation and genomic damage in vitro in cell lines with different expression of the insulin and the IGF-1 receptors after treating the cells with insulin and the insulin analog glargine...
November 22, 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27876558/prevalidation-trial-for-a-novel-in-vitro-eye-irritation-test-using-the-reconstructed-human-cornea-like-epithelial-model-mctt-hce%C3%A2
#3
Hyeri Yang, Da-Eun Kim, Won-Hee Jang, Susun An, Sun-A Cho, Mi-Sook Jung, Ji Eun Lee, Kyung-Wook Yeo, Sang Bum Koh, Tae-Cheon Jeong, Mi-Jeong Kang, Young-Jin Chun, Su-Hyon Lee, Kyung-Min Lim, SeungJin Bae
Here, we report the results of a prevalidation trial for an in vitro eye irritation test (EIT) using the reconstructed human cornea-like epithelium, MCTT HCE™. The optimal cutoff to determine irritation in the prediction model was established at 35% with the receiver operation characteristics(ROC) curve for 126 substances. Within-lab(WL) and between-lab(BL) reproducibility was tested for 20 reference substances by 3 participating laboratories. Viability data described by mean±SD or ±1/2 difference between duplicate wells, and scatter plots, demonstrated the WL/BL consistency...
November 19, 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27865774/a-systems-toxicology-approach-for-comparative-assessment-biological-impact-of-an-aerosol-from-a-candidate-modified-risk-tobacco-product-and-cigarette-smoke-on-human-organotypic-bronchial-epithelial-cultures
#4
Anita R Iskandar, Carole Mathis, Walter K Schlage, Stefan Frentzel, Patrice Leroy, Yang Xiang, Alain Sewer, Shoaib Majeed, Laura Ortega-Torres, Stephanie Johne, Emmanuel Guedj, Keyur Trivedi, Gilles Kratzer, Celine Merg, Ashraf Elamin, Florian Martin, Nikolai V Ivanov, Manuel C Peitsch, Julia Hoeng
This study reports a comparative assessment of the biological impact of a heated tobacco aerosol from the tobacco heating system (THS) 2.2 and smoke from a combustible 3R4F cigarette. Human organotypic bronchial epithelial cultures were exposed to an aerosol from THS2.2 (a candidate modified-risk tobacco product) or 3R4F smoke at similar nicotine concentrations. A systems toxicology approach was applied to enable a comprehensive exposure impact assessment. Culture histology, cytotoxicity, secreted pro-inflammatory mediators, ciliary beating, and genome-wide mRNA/miRNA profiles were assessed at various time points post-exposure...
November 16, 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27845199/in-vitro-toxicity-of-pirimiphos-methyl-in-atlantic-salmon-hepatocytes
#5
Pål A Olsvik, Marc H G Berntssen, Liv Søfteland
Recent screening of Atlantic salmon feeds has uncovered residues of several pesticides, including pirimiphos-methyl. Pirimiphos-methyl is an organophosphate (OP) insecticide, causing inhibition of acetylcholinesterase in target organisms. The aim of this study was to examine pirimiphos-methyl non-targeted mode of action toxicity in Atlantic salmon using in vitro exposure. Hepatocyte cells were exposed to pirimiphos-methyl (control-0.1-1.0-10-100-1000μM) for 48h. Transcriptomics (RNA-seq) and non-targeted metabolomics were used to screen for effects of the pesticide...
November 12, 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27836570/metabolic-induced-cytotoxicity-of-diosbulbin-b-in-cyp3a4-expressing-cells
#6
Ji-Zong Jiang, Bao-Hua Yang, Li-Li Ji, Li Yang, Yu-Chang Mao, Zhuo-Han Hu, Zheng-Tao Wang, Chang-Hong Wang
As a candidate antitumor agent, diosbulbin B (DB) can induce serious liver toxicity and other adverse reactions. DB is mainly metabolized by CYP3A4 in vitro and in vivo, but the cytotoxicity and anti-tumor mechanisms of DB have yet to be clarified. This study aimed to determine whether the cytotoxicity and anti-tumor effects of DB are related to the metabolism-induced activation of CYP3A4 in various cell models, including CYP-free NIH3T3 cells, primary rat hepatocytes, HepG2 and L02 cells of high CYP3A4 expression and wild-type...
November 9, 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27836571/effect-of-acrylamide-induced-neurotoxicity-in-a-primary-astrocytes-microglial-co-culture-model
#7
Mengyao Zhao, Fu Sheng Lewis Wang, Xiao Song Hu, Fang Chen, Hing Man Chan
Acrylamide (AA),is acommon food contaminant generated by heat processing. Astrocytes and microglia are the two major glial cell types in the brain that play pivotal but different roles in maintaining optimal brain function. The objective of this study is to investigate the neurotoxicity of AA, using a primary astrocytes/microglia co-culture model. Co-cultural cells obtained from Balb/c mice were cultured and treated with 0-1.0mM AA for 24-96h. Cell viability, reactive oxygen species (ROS) generation, oxidative end produces formation and glutathione (GSH) levels were measured...
November 8, 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27829164/molecular-effects-of-1-naphthyl-methylcarbamate-and-solar-radiation-exposures-on-human-melanocytes
#8
Bianca Ferrucio, Manoela Tiago, Richard D Fannin, Liwen Liu, Kevin Gerrish, Silvya Stuchi Maria-Engler, Richard S Paules, Silvia Berlanga de Moraes Barros
Carbaryl (1-naphthyl-methylcarbamate), a broad-spectrum insecticide, has recently been associated with the development of cutaneous melanoma in an epidemiological cohort study with U.S. farm workers also exposed to ultraviolet radiation, the main etiologic factor for skin carcinogenesis. We hypothesized that carbaryl exposure may increase deleterious effects of UV solar radiation on skin melanocytes. This study aimed to characterize human melanocytes after individual or combined exposure to carbaryl (100μM) and solar radiation (375mJ/cm(2))...
November 6, 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27825932/evaluation-of-pfos-mediated-neurotoxicity-in-rat-primary-neurons-and-astrocytes-cultured-separately-or-in-co-culture
#9
Zhenwei Li, Qi Liu, Chang Liu, Chunna Li, Yachen Li, Shuangyue Li, Xiaohui Liu, Jing Shao
Perfluorooctane sulfonate (PFOS) is a potential neurotoxicant reported by epidemiological investigations and experimental studies, while the underlying mechanisms are still unclear. Astrocytes not only support for the construction of neurons, but also conduct neuronal functions through glutamate-glutamine cycle in astrocyte-neuron crosstalk. In the present study, the effect of PFOS exposure on rat primary hippocampal neurons or cortex astrocytes was evaluated. Then the role of the astrocytes in PFOS-induced toxic effect on neurons was explored with astrocyte-neuron co-culture system...
November 5, 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27825931/quantitative-analysis-of-gene-expression-changes-in-response-to-genotoxic-compounds
#10
Ceri A Morris, Gamal A El-Hiti, Ian Weeks, Stuart Woodhead, Keith Smith, Peter Kille
Techniques that quantify molecular endpoints sufficiently sensitive to identify and classify potentially toxic compounds have wide potential for high-throughput in vitro screening. Expression of three genes, RAD51C, TP53 and cystatin A (CSTA), in HEPG2 cells was measured by Q-PCR amplification. In parallel, we developed alternative assays for the same 3 gene signature based on an acridinium-ester chemiluminescent reporter molecule. HEPG2 cells were challenged with eighteen different compounds (n=18) chosen to represent compounds that are genotoxic (n=8), non-genotoxic non-carcinogenic (n=2) or have a less well defined mechanism of action with respect to genotoxicity (n=8)...
November 5, 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27825933/comparative-toxicity-oxidative-stress-and-endocrine-disruption-potential-of-plasticizers-in-jeg-3-human-placental-cells
#11
Elisabet Pérez-Albaladejo, Denise Fernandes, Silvia Lacorte, Cinta Porte
Plasticizers are suspected to be toxic and/or to modulate or disrupt the endocrine system of humans and to cross the placental barrier, being embryonic and fetal development a particularly vulnerable period. This work investigates the comparative toxicity and ability to interfere with the synthesis of steroids and to generate reactive oxygen species (ROS) of a selected number of plasticizers, including bisphenol A (BPA), nonyl- (NP) and octylphenol (OP), benzyl butyl phthalate (BBP), dibutyl phthalate (DBP), di(2-ethylhexyl)phthalate (DEHP) and dimethyl phthalate (DMP), in the human placenta JEG-3 cells...
November 4, 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27825930/novel-approach-to-study-the-cardiovascular-effects-and-mechanism-of-action-of-urban-particulate-matter-using-lung-epithelial-endothelial-tetra-culture-system
#12
Ha Ryong Kim, Han Soo Cho, Da Young Shin, Kyu Hyuck Chung
In vitro models have become increasingly sophisticated, and their usefulness in supporting toxicity testing is well established. The present study was designed to establish a novel in vitro model that mimics the cellular network surrounding airways and pulmonary blood vessels, to study the cardiovascular toxic effects of particulate matter (PM). Transwell culture method was used to develop a novel tetra-culture system consisting of tri-cultures (one lung epithelial and two immune cell lines) in the apical chamber and endothelial cells in the basolateral chamber...
November 4, 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27816504/nadph-oxidase-nox-1-mediates-cigarette-smoke-induced-superoxide-generation-in-rat-vascular-smooth-muscle-cells
#13
Kyung-Hwa Chang, Jung-Min Park, Chang Hoon Lee, Bumseok Kim, Kyung-Chul Choi, Seong-Jin Choi, Kyuhong Lee, Moo-Yeol Lee
Smoking is a well-established risk factor for cardiovascular diseases. Oxidative stress is one of the common etiological factors, and NADPH oxidase (NOX) has been suggested as a potential mediator of oxidative stress. In this study, cigarette smoke (CS)-induced superoxide production was characterized in vascular smooth muscle cells (VSMC). CS was prepared in forms of cigarette smoke extract (CSE) and total particulate matter (TPM). Several molecular probes for reactive oxygen species were trialed, and dihydroethidium (DHE) and WST-1 were chosen for superoxide detection considering the autofluorescence, light absorbance, and peroxidase inhibitory activity of CS...
November 3, 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27816503/silver-nanoparticles-significance-of-physicochemical-properties-and-assay-interference-on-the-interpretation-of-in-vitro-cytotoxicity-studies
#14
REVIEW
Kausar B Riaz Ahmed, Amber M Nagy, Ronald P Brown, Qin Zhang, Subhas G Malghan, Peter L Goering
Silver nanoparticles (AgNPs) have generated a great deal of interest in the research, consumer product, and medical product communities due to their antimicrobial and anti-biofouling properties. However, in addition to their antimicrobial action, concerns have been expressed about the potential adverse human health effects of AgNPs. In vitro cytotoxicity studies often are used to characterize the biological response to AgNPs and the results of these studies may be used to identify hazards associated with exposure to AgNPs...
November 3, 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27806920/casearin-d-inhibits-erk-phosphorylation-and-induces-downregulation-of-cyclin-d1-in-hepg2-cells
#15
Guilherme Álvaro Ferreira-Silva, Carla Carolina Lopes Lages, Patricia Sartorelli, Flávia Rie Hasegawa, Marisi Gomes Soares, Marisa Ionta
Cancer is a public health problem which represents the second cause of death in the world. In this framework, it is necessary to identify novel compounds with antineoplastic potential. Plants are an important source for discovering novel compounds with pharmacological potential. In this study, we aimed to investigate the antiproliferative potential of isolated compounds from Casearia sylvestris on tumor cell lines. Crude extract effectively reduced cell viability of 4 tumor cell lines (HepG2, A549, U251-MG, and HT-144) after 48h treatment...
October 30, 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27794450/3d-engineered-in-vitro-hepatospheroids-for-studying-drug-toxicity-and-metabolism
#16
Swati Chitrangi, Prabha Nair, Aparna Khanna
Drug toxicity is one of the reasons for late stage drug attrition, because of hepatotoxicity. Various in vitro liver models like primary human hepatocytes, immortalized human hepatic cell lines, liver slices and microsomes have been used; but limited by viability, hepatic gene expression and function. The 3D-engineered construct of hepatocyte-like-cells (HLCs) differentiated from stem cells, may provide a limitless source of hepatocytes with improved reproducibility. Towards this end, we used hepatospheroids (diameter=50-80μm) differentiated from human-umbilical-cord-mesenchymal stem cells (hUC-MSCs) on 3D scaffold GEVAC (Gelatin-vinyl-acetate-copolymer) as in vitro model for studying drug metabolism/toxicity...
October 26, 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27784537/corrigendum-to-identification-of-novel-reaction-products-of-methylene-bis-phenylisocyanate-mdi-with-oxidized-glutathione-in-aqueous-solution-and-also-during-incubation-of-mdi-with-a-murine-hepatic-s9-fraction-toxicol-in-vitro-2016-97-104
#17
A V Wisnewski, J Liu, A F Nassar
No abstract text is available yet for this article.
October 23, 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27751951/further-development-of-an-in-vitro-model-for-studying-the-penetration-of-chemicals-through-compromised-skin
#18
Diane J Davies, Jon R Heylings, Heather Gayes, Timothy J McCarthy, M Catherine Mack
A new in vitro model based on the electrical resistance properties of the skin barrier has been established in this laboratory. The model utilises a tape stripping procedure in dermatomed pig skin that removes a specific proportion of the stratum corneum, mimicking impaired barrier function observed in humans with damaged skin. The skin penetration and distribution of chemicals with differing physicochemical properties, namely; Benzoic acid, 3-Aminophenol, Caffeine and Sucrose has been assessed in this model...
October 14, 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27746372/practical-use-of-the-virtual-cell-based-assay-simulation-of-repeated-exposure-experiments-in-liver-cell-lines
#19
A Paini, M Mennecozzi, T Horvat, K Gerloff, T Palosaari, J V Sala Benito, A Worth
The Virtual Cell Based Assay (VCBA) was applied to simulate the long-term (repeat dose) toxic effects of chemicals, including substances in cosmetics and personal care products. The presented model is an extension of the original VCBA for simulation of single exposure and is implemented in a KNIME workflow. This work illustrates the steps taken to simulate the repeated dose effects of two reference compounds, caffeine and amiodarone. Using caffeine, in vitro experimental viability data in single exposure from two human liver cell lines, HepG2 and HepaRG, were measured and used to optimize the VCBA, subsequently repeated exposure simulations were run...
October 14, 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27746373/establishment-of-a-new-teleost-brain-cell-line-dlb-1-from-the-european-sea-bass-and-its-use-to-study-metal-toxicology
#20
Patricia Morcillo, Elena Chaves-Pozo, José Meseguer, M Ángeles Esteban, Alberto Cuesta
In teleost fish, there are no commercial cell lines for the European sea bass (Dicentrarchus labrax). Thus, we have established the sea bass brain (DLB-1) cell line, using a fish retrovirus for immortalization, which resemble epithelial cells and express glial cells markers. Exposure to metals [Cd, methylmercury (MeHg), Pb or As] produces cytotoxicity and induction of reactive oxygen species (ROS) production. Interestingly, cell cycle analysis of DLB-1 cells shows that exposure to metals alters it significantly...
October 13, 2016: Toxicology in Vitro: An International Journal Published in Association with BIBRA
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