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Toxicology in Vitro: An International Journal Published in Association with BIBRA

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https://www.readbyqxmd.com/read/29331636/cell-wall-damage-and-oxidative-stress-in-candida-albicans-atcc10231-and-aspergillus-niger-caused-by-palladium-nanoparticles
#1
Martha Samira Athie-García, Hilda Amelia Piñón-Castillo, Laila Nayzzel Muñoz-Castellanos, Ana Laura Ulloa-Ogaz, Perla Ivonne Martínez-Varela, Armando Quintero-Ramos, Robert Duran, José Guadalupe Murillo-Ramirez, Erasmo Orrantia-Borunda
In this work the toxic effect of Palladium nanoparticles (PdNPs) was investigated in two eukaryotic cell models, Candida albicans and Aspergillus niger. PdNPs were synthesized by chemical reduction method, obtaining spherical NPs with a primary size ranging from 3 to 15 nm. PdNPs showed a hydrodynamic size of 1548 nm in Lee's minimum media. Minimal inhibitory concentration was determined at 200 and 250 ppm for Candida albicans and Aspergillus niger respectively, revealing a significant cell growth inhibition (ANOVA and tukey analysis, α = 0...
January 10, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29326048/round-robin-study-to-evaluate-the-reconstructed-human-epidermis-rhe-model-as-an-in-vitro-skin-irritation-test-for-detection-of-irritant-activity-in-medical-device-extracts
#2
Wim H De Jong, Sebastian Hoffmann, Michelle Lee, Helena Kandárová, Christian Pellevoisin, Yuji Haishima, Beau Rollins, Austin Zdawzcyk, Jamin Willoughby, Michael Bachelor, Timothy Schatz, Shelby Skoog, Sherry Parker, Anita Sawyer, Paolo Pescio, Kristina Fant, Kwang-Mahn Kim, Jae Sung Kwon, Helge Gehrke, Hana Hofman-Hüther, Marisa Meloni, Conrad Julius, Damien Briotet, Sylvia Letasiova, Reiko Kato, Atsuko Miyajima, Liset De La Fonteyne, Christelle Videau, Carine Tornier, Audrey Turley, Nicholas Christiano, Thor Rollins, Kelly P Coleman
No abstract text is available yet for this article.
January 8, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29309803/effect-of-cyclosporine-tacrolimus-and-sirolimus-on-cellular-senescence-in-renal-epithelial-cells
#3
Christian Koppelstaetter, Georg Kern, Gisela Leierer, Sabine Mair, Gert Mayer, Johannes Leierer
INTRODUCTION: In transplantation medicine calcineurin inhibitors (CNI) still represent the backbone of immunosuppressive therapy. The nephrotoxic potential of the CNI Cyclosporine A (CsA) and Tacrolimus (FK506) is well recognized and CNI not only have been linked with toxicity, but also with cellular senescence which hinders parenchymal tissue regeneration and thus may prime kidneys for subsequent insults. To minimize pathological effects on kidney grafts, alternative immunosuppressive agents like mTOR inhibitors or the T-cell co-stimulation blocker Belatacept have been introduced...
January 5, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29307701/toxicological-effects-of-regulated-mycotoxins-and-persistent-organochloride-pesticides-in-vitro-cytotoxic-assessment-of-single-and-defined-mixtures-on-ma-10-murine-leydig-cell-line
#4
Ukpai A Eze, John Huntriss, Michael N Routledge, Yun Yun Gong
Epidemiological studies show that there is global decline in male fertility primarily as a result of poor sperm quality and this is attributed to exposure to endocrine disrupting chemicals (EDCs) in the environment, food and pharmaceutical products, including mycotoxins and pesticides. The Leydig cells in the male testes are responsible for producing androgens, hormones that play major roles in male development and reproductive function. Therefore, any toxin that affects the function and morphology of the Leydig cells may result in sub-fertility or infertility...
January 4, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29305980/predictive-performance-and-inter-laboratory-reproducibility-in-assessing-eye-irritation-potential-of-water-and-oil-soluble-mixtures-using-the-short-time-exposure-test-method
#5
Takayuki Abo, Allison Hilberer, Christine Behle-Wagner, Mika Watanabe, David Cameron, Annette Kirst, Yuko Nukada, Takuo Yuki, Daisuke Araki, Hitoshi Sakaguchi, Hiroshi Itagaki
The Short Time Exposure (STE) test method is an alternative method for assessing eye irritation potential using Statens Seruminstitut Rabbit Cornea cells and has been adopted as test guideline 491 by the Organisation for Economic Co-operation and Development. Its good predictive performance in identifying the Globally Harmonized System (GHS) No Category (NC) or Irritant Category has been demonstrated in evaluations of water-soluble substances, oil-soluble substances, and water-soluble mixtures. However, the predictive performance for oil-soluble mixtures was not evaluated...
January 3, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29288082/octyl-gallate-reduces-atp-levels-and-ki67-expression-leading-hepg2-cells-to-cell-cycle-arrest-and-mitochondria-mediated-apoptosis
#6
Kelly Goulart Lima, Gabriele Catyana Krause, Elisa Feller Gonçalves da Silva, Léder Leal Xavier, Leo Anderson Meira Martins, Laura Manzoli Alice, Luiza Bueno da Luz, Rodrigo Benedetti Gassen, Eduardo Cremonese Filippi-Chiela, Gabriela Viegas Haute, Maria Claudia Rosa Garcia, Giselle Afonso Funchal, Leonardo Pedrazza, Camille Kirinus Reghelin, Jarbas Rodrigues De Oliveira
Octyl gallate (OG) is an antioxidant that has shown anti-tumor, anti-diabetic and anti-amyloidogenic activities. Mitochondria play an important role in hepatocellular carcinoma, mainly by maintaining accelerated cellular proliferation through the production of ATP. Thus, the mitochondria may be a target for antitumor therapies. Here, we investigated the effects of OG in the hepatocarcinoma cell line (HepG2) and the mechanisms involved. We report, for the first time, that treatment with OG for 24h inhibited HepG2 cell growth by decreasing mitochondrial activity and mass, which led to the reduction of ATP levels...
December 26, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29278758/leucine-reduces-the-proliferation-of-mc3t3-e1-cells-through-dna-damage-and-cell-senescence
#7
Raquel da Luz Dias, Bruno Basso, Márcio Vinícius Fagundes Donadio, Francesc Ventura Pujol, Ramón Bartrons, Gabriela Viegas Haute, Rodrigo Benedetti Gassen, Henrique Dias Bregolin, Gabriele Krause, Cassiana Viau, Jenifer Saffi, Fernanda Bordignon Nunes, José Luis Rosa, Jarbas Rodrigues de Oliveira
Leucine (Leu) is an essential branched-chain amino acid, present in dairy products, which has been investigated for its important role in cell signaling. The effects of Leu on several kinds of cells have been studied, altough little is known on its action upon bone cells and cell proliferation. Thus, the aim of this study is to investigate the effects of Leu supplementation on the proliferation of pre-osteoblasts from MC3T3-E1 lineage. MC3T3-E1 cells were kept in Alpha medium supplemented with 10% fetal bovine serum and 1% antibiotic-antimitotic...
December 23, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29277654/alternative-approaches-for-acute-inhalation-toxicity-testing-to-address-global-regulatory-and-non-regulatory-data-requirements-an-international-workshop-report
#8
Amy J Clippinger, David Allen, Annie M Jarabek, Marco Corvaro, Marianna Gaça, Sean Gehen, Jon A Hotchkiss, Grace Patlewicz, Jodie Melbourne, Paul Hinderliter, Miyoung Yoon, Dan Huh, Anna Lowit, Barbara Buckley, Michael Bartels, Kelly BéruBé, Daniel M Wilson, Ian Indans, Mathieu Vinken
Inhalation toxicity testing, which provides the basis for hazard labeling and risk management of chemicals with potential exposure to the respiratory tract, has traditionally been conducted using animals. Significant research efforts have been directed at the development of mechanistically based, non-animal testing approaches that hold promise to provide human-relevant data and an enhanced understanding of toxicity mechanisms. A September 2016 workshop, "Alternative Approaches for Acute Inhalation Toxicity Testing to Address Global Regulatory and Non-Regulatory Data Requirements", explored current testing requirements and ongoing efforts to achieve global regulatory acceptance for non-animal testing approaches...
December 22, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29277653/microrna-191-regulated-by-hif-2%C3%AE-is-involved-in-emt-and-acquisition-of-a-stem-cell-like-phenotype-in-arsenite-transformed-human-liver-epithelial-cells
#9
Chao Chen, Qianlei Yang, Dapeng Wang, Fei Luo, Xinlu Liu, Junchao Xue, Ping Yang, Hui Xu, Jiachun Lu, Aihua Zhang, Qizhan Liu
Inorganic arsenic is widely distributed in the environment, and epidemiologic data show a strong association between arsenic exposure and risk of liver cancer. An understanding of the mechanisms underlying development of liver cancer and metastasis would be useful in reducing the incidence and mortality of liver cancer. MicroRNAs (miRs) act as regulators in liver cancer. Here, we show that acute or chronic exposure of human liver epithelial L-02 cells to arsenite increased expression of miR-191. There were decreased levels of BASP-1 and E-cadherin and increased levels of WT-1 and N-cadherin, indicating that arsenite induced epithelial-mesenchymal transition (EMT)...
December 22, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29275038/dermal-absorption-of-testosterone-in-human-and-pig-skin-in-vitro
#10
Jon R Heylings, Diane J Davies, Rebecca Burton
The OECD test guideline 428 for the assessment of dermal absorption in vitro has been in force for more than a decade. Various sectors of industry utilise the method for the registration of chemical products. These include the Agrochemical and Cosmetic sectors where the OECD test guideline and industry-specific guidance forms a key part of the human risk assessment process for new and existing products. This investigation has compared the dermal absorption characteristics of one of the OECD 428 reference chemicals, testosterone, in human and pig dermatomed skin...
December 21, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29273543/acute-effects-of-lead-on-porcine-neonatal-sertoli-cells-in-vitro
#11
Francesca Mancuso, Iva Arato, Cinzia Lilli, Catia Bellucci, Maria Bodo, Mario Calvitti, Maria Chiara Aglietti, Marco dell'Omo, Claudio Nastruzzi, Riccardo Calafiore, Giovanni Luca, Lorella Marinucci
Environmental pollution is one of the main factors responsible for reducing fertility in males. Lead is one of the major heavy metal contaminants that impairs several organs; it preferentially accumulates in male reproductive organs and alters sperm quality both in vivo and in vitro. However, the underlying mechanisms remain unclear. Sertoli cells (SCs) provide structural and physiological support to spermatogenic cells within seminiferous tubules. Therefore, changes in SCs affect the developing germ cells and alter spermatogenesis...
December 19, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29269118/characterization-of-3d-embryonic-c57bl-6-and-a-j-mouse-midbrain-micromass-in-vitro-culture-systems-for-developmental-neurotoxicity-testing
#12
Julie Juyoung Park, Brittany A Weldon, Sungwoo Hong, Tomomi Workman, William C Griffith, Julie H Park, Elaine M Faustman
In vitro micromass culture systems have been proposed as an alternative method for developmental toxicity assessment to reduce the need for resource-intensive in vivo toxicity testing. In this study, a three-dimensional in vitro embryonic mouse midbrain culture system is characterized in two mouse strains to facilitate gene x environment considerations. Gestational day (GD) 11 C57BL/6 or GD 12 A/J mouse midbrain cells were isolated and cultured in high-density micromass format for 22days in vitro (DIV). Hematoxylin intensity and protein content revealed that neuronal differentiation increases linearly over time in both C57BL/6 and A/J cultures...
December 18, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29262311/cytotoxicity-of-demalonyl-thyrsiflorin-a-a-semisynthetic-labdane-derived-diterpenoid-to-melanoma-cells
#13
V Cardile, R Avola, A C E Graziano, M Piovano, A Russo
Diterpenes are compounds with complex structure and due to their unique carbon skeleton and interesting biological activities, have been the focus of continuous studies for the development of new anticancer agents. The plants of the genus Calceolaria (Scrophulariaceae family), native of South America have also yielded several new diterpenes with the scopadulane skeleton, such as thyrsiflorin A. The present study was undertaken to investigate the effect of the semisynthetic compound, demalonyl thyrsiflorin A on human melanoma cells...
December 17, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29262310/binding-of-cholera-toxin-b-subunit-to-intestinal-epithelial-cells
#14
Elena V Navolotskaya, Vladimir B Sadovnikov, Valery M Lipkin, Vladimir P Zav'yalov
We have prepared 125I-labeled cholera toxin B subunit (125I-labeled CT-B, a specific activity of 98Ci/mmol) and found that it binds to rat IEC-6 and human Caco-2 intestinal epithelial cells with high affinity (Kd 3.6 and 3.7nM, respectively). The binding of labeled protein was completely inhibited by unlabeled thymosin-α1 (TM-α1), interferon-α2 (IFN-α2), and the synthetic peptide LKEKK that corresponds to residues 16-20 in TM-α1 and 131-135 in IFN-α2, but was not inhibited by the synthetic peptide KKEKL with inverted amino acid sequence (Ki>10μM)...
December 17, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29258884/drug-toxicity-profiling-of-a-saccharomyces-cerevisiae-deubiquitinase-deletion-panel-shows-that-acetaminophen-mimics-tyrosine
#15
Angelina Huseinovic, Marc van Dijk, Nico P E Vermeulen, Fred van Leeuwen, Jan M Kooter, J Chris Vos
Post-translational protein modification by addition or removal of the small polypeptide ubiquitin is involved in a range of critical cellular processes, like proteasomal protein degradation, DNA repair, gene expression, internalization of membrane proteins, and drug sensitivity. We recently identified genes important for acetaminophen (APAP) toxicity in a comprehensive screen and our findings suggested that a small set of yeast strains carrying deletions of ubiquitin-related genes can be informative for drug toxicity profiling...
December 16, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29248593/phototoxic-effect-of-oxytetracycline-on-normal-human-melanocytes
#16
Jakub Rok, Dorota Wrześniok, Artur Beberok, Michał Otręba, Marcin Delijewski, Ewa Buszman
Oxytetracycline is a broad-spectrum antibiotic, used in dermatology and veterinary medicine. Like other tetracyclines, it may evoke skin phototoxic reactions related to generation of reactive oxygen species (ROS). Melanins are biopolymers synthesised in melanocytes - highly specialised cells, localised in the basal layer of epidermis. Production of melanin is a defence mechanism against harmful effects of UV radiation, ROS and many chemical substances, including drugs. In the present study the influence of oxytetracycline and UVA radiation on darkly pigmented melanocytes viability, the melanogenesis process and the activity of antioxidant enzymes were analysed...
December 14, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29248592/fluorochloridone-induces-primary-cultured-sertoli-cells-apoptosis-involvement-of-ros-and-intracellular-calcium-ions-mediated-erk1-2-activation
#17
Luqing Liu, Xiuli Chang, Yubin Zhang, Chunhua Wu, Rui Li, Liming Tang, Zhijun Zhou
Fluorochloridone (FLC) is a widely used pyrrolidone selective herbicide and reported to induce testis injuries in male rats, but the underlying mechanism is largely unknown. In the present study, primary-cultured Sertoli cells were exposed to FLC at the concentration of 0-10.00μM to study the mechanism of FLC-induced apoptosis. The roles of ROS, intracellular calcium, endoplasmic reticulum (ER), and ERK1/2 were looked at with ROS scavenger N-acetyl-cysteine (NAC), intracellular calcium chelator BAPTA-AM, ER calcium depleting agent thapsigargin (TG), and ERK1/2 inhibitor U0126, respectively...
December 14, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29247761/genotoxicity-assessment-of-biphasic-calcium-phosphate-of-modified-porosity-on-human-dental-pulp-cells-using-ames-and-comet-assays
#18
Nur Fathin Alia Che Wahab, Thirumulu Ponnuraj Kannan, Zuliani Mahmood, Ismail Ab Rahman, Hanafi Ismail
Biphasic Calcium Phosphate (BCP) with a ratio of 20/80 Hydroxyapatite (HA)/Beta-tricalcium phosphate (β-TCP) promotes the differentiation of human dental pulp cells (HDPCs). In the current study, the genotoxicity of locally produced BCP of modified porosity (65%) with a mean pore size of 300micrometer (μm) was assessed using Comet and Ames assays. HDPCs were treated with BCP extract at three different inhibitory concentrations which were obtained based on cytotoxicity test conducted with concurrent negative and positive controls...
December 13, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29229420/ros-mediated-oligomerization-of-vdac2-is-associated-with-quinocetone-induced-apoptotic-cell-death
#19
Xiayun Yang, Shusheng Tang, Daowen Li, Bin Li, Xilong Xiao
Quinocetone (QCT) has been approved and widely used as an animal feed additive in China since 2003. However, investigations indicate that QCT shows potential toxicity both in vitro and in vivo. Although voltage dependent anion channel 1 (VDAC1) involved in regulating QCT-induced apoptotic cell death has been established, the role of voltage dependent anion channel 2 (VDAC2) in QCT-induced toxicity remains unclear. In this study, we showed that QCT-induced cell death was coupled to VDAC2 oligomerization. Moreover, VDAC inhibitor 4, 4'-diisothiocyano stilbene-2, 2'-disulfonic acid (DIDS) alleviated QCT-induced cell death and VDAC2 oligomerization...
December 8, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29223574/evaluation-of-the-effects-of-antiepileptic-drugs-on-folic-acid-uptake-by-human-placental-choriocarcinoma-cells
#20
Yuko Kurosawa, Ayako Furugen, Ayako Nishimura, Katsuya Narumi, Masaki Kobayashi, Ken Iseki
Folate status during pregnancy is important for fetal development and health. The placenta plays an important role in supplying the fetus with folate. Most women with epilepsy continue their medication during pregnancy. In the present study, we aimed to evaluate the effects of 16 antiepileptic drugs, clinically used for treatment of epilepsy, on folic acid uptake in two in vitro placental models, BeWo and JEG-3 cells. Short-term exposure to antiepileptic drugs had no effects on [3H]-folic acid uptake by BeWo cells...
December 6, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
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