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Journals Toxicology in Vitro : An Inter...

Toxicology in Vitro : An International Journal Published in Association with BIBRA

https://read.qxmd.com/read/38484921/transcriptional-landscape-of-human-keratinocyte-models-exposed-to-60-ghz-millimeter-waves
#1
JOURNAL ARTICLE
Catherine Martin, Bertrand Evrard, Frederic Percevault, Kate Ryder, Thomas Darde, Aurélie Lardenois, Maxim Zhadobov, Ronan Sauleau, Frédéric Chalmel, Yves Le Dréan, Denis Habauzit
The use of millimeter waves (MMW) will exponentially grow in the coming years due to their future utilization in 5G/6G networks. The question of possible biological effects at these frequencies has been raised. In this present study, we aimed to investigate gene expression changes under exposure to MMW using the Bulk RNA Barcoding and sequencing (BRB-seq) technology. To address this issue, three exposure scenarios were performed aiming at: i) comparing the cellular response of two primary culture of keratinocytes (HEK and NHEK) and one keratinocyte derivate cell line (HaCaT) exposed to MMW; ii) exploring the incident power density dose-effect on gene expression in HaCaT cell line; and, iii) studying the exposure duration at the new ICNIRP exposure limit for the general population...
March 12, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38479708/metformin-and-buparlisib-synergistically-induce-apoptosis-of-non-small-lung-cancer-nsclc-cells-via-akt-foxo3a-puma-axis
#2
JOURNAL ARTICLE
Wang Shanshan, Ma Hongying, Fang Jingjing, Yu Rui
Non-small cell lung cancer (NSCLC) is a global health issue lacking effective treatments. Buparlisib is a pan-PI3K inhibitor that shows promising clinical results in treating NSCLC. However, chemoresistance is inevitable and hampers the application of buparlisib. Studies show that a combination of phytochemicals and chemotherapeutics enhances its effectiveness. Here, we evaluated the role of metformin, an agent with multiple pharmacological properties, in enhancing the anti-tumour activities of buparlisib against NSCLC cells...
March 11, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38458500/simulated-gastric-leachate-of-3d-printer-metal-fill-filaments-induces-cytotoxic-effects-in-rat-and-human-intestinal-models
#3
JOURNAL ARTICLE
Michael F Hughes, Haley M Clapper, Getachew Tedla, Tyler D Sowers, Kim R Rogers
Metals are used in 3-dimensional (3D) printer filaments in the manufacture of 3D printed objects. Exposure to the filaments, printed objects and emissions from printing may pose health risks from release of toxic metals. This study investigated the cytotoxicity of extruded 3D printer filament leachates in rat and human intestinal cells. Copper-, bronze-, and steel-fill extruded filaments were incubated in acidic media for 2 h. Leachates were adjusted to pH 7 and cells exposed for 4 or 24 h...
March 6, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38458499/in-vitro-assessment-of-cytotoxicity-of-spent-fluid-catalytic-cracking-refinery-catalysts-on-cell-lines-and-identification-of-critical-toxic-metals
#4
JOURNAL ARTICLE
Yue-Jie Wang, Zhi-Guo Sheng, Jun Li, Zhen-Feng Zhao, Ling-Ling Li, Chen Shen
The Purpose of the present study was to quantify the responses of ten cell lines (HeLa, HepG2, HEK293, MDA-MB-231, A498, A549, A357, 3 T3, BALB-C3 T3, and NIH-3 T3) to spent fluid catalytic cracking catalysts (SFCCCs) from different petroleum refineries, and relate these responses to metal concentrations of SFCCC leachates (SFCCCLs). Cytotoxicity of SFCCCs were significantly different depending on cell lines. A357 and 3 T3 cell were the most sensitive, and A498 and HeLa cells were the least sensitive...
March 6, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38447685/transcriptomic-profile-of-human-ipsc-derived-podocyte-like-cells-exposed-to-a-panel-of-xenobiotics
#5
JOURNAL ARTICLE
Cormac Murphy, Paul Jennings, Anja Wilmes
Podocytes play a critical role in the formation and maintenance of the glomerular filtration barrier and injury to these cells can lead to a breakdown of the glomerular barrier causing permanent damage leading to progressive chronic kidney disease. Matured podocytes have little proliferative potential, which makes them critical cells from a health perspective, but also challenging cells to maintain in vitro. Differentiating podocyte-like cells from induced pluripotent stem cells (iPSC) cells provides a novel and continuous source of cells...
March 4, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38432573/lovastatin-impairs-cellular-proliferation-and-enhances-hyaluronic-acid-production-in-fibroblast-like-synoviocytes
#6
JOURNAL ARTICLE
Wen-Tien Wu, Chen-Chie Wang, Dai-Hua Lu, Kuan-Jung Lu, Yu-Chia Chang, Kai-Chiang Yang
INTRODUCTION: Statins have demonstrated chondroprotective effects by reducing inflammation and mitigating extracellular matrix degradation. However, statins are also reported to be cytotoxic to several types of cells. Early-onset osteoarthritis (OA) is characterized by synovial inflammation, which adversely affects hyaluronan (HA) production in fibroblast-like synoviocytes (FLSs). Nevertheless, the precise effects of statins on the synovium remain unclear. METHODS: This study investigated the impact of lovastatin on human FLSs, and HA secretion-related genes, signaling pathways, and production were evaluated...
March 1, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38431060/using-cell-culture-systems-from-the-persian-gulf-arabian-yellowfin-sea-bream-acanthopagrus-arabicus-to-assess-the-effects-of-dexamethasone-on-gonad-and-brain-aromatase-activity-and-steroid-production
#7
JOURNAL ARTICLE
Zahra Balasem, Negin Salamat, Hoda Mojiri-Forushani
Dexamethasone (DEX) is a synthetic glucocorticoid widely used as pharmaceutical and usually exists in effluents with varying degrees of concentrations. In this study, cultivated Brain, ovary and testis cells from Arabian Sea bream, Acanthopagrus arabicus, were treated by DEX at concentrations of 0, 0.3, 3.0, 30.0 and 300.0 μg/ml for 48 h. The aromatase activity and steroid (17-β-estradiol (E2), progesterone (P) and testosterone (T)) production by cells were measured at 12, 24 and 48 h of the experiment...
February 29, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38431059/characterization-of-palmitic-acid-toxicity-induced-insulin-resistance-in-hepg2-cells
#8
JOURNAL ARTICLE
Sajad Malik, Shrirang Inamdar, Jhankar Acharya, Pranay Goel, Saroj Ghaskadbi
BACKGROUND: An etiology of palmitic acid (PA) induced insulin resistance (IR) is complex for which two mechanisms are proposed namely ROS induced JNK activation and lipid induced protein kinase-C (PKCε) activation. However, whether these mechanisms act alone or in consortium is not clear. METHODS AND RESULTS: In this study, we have characterized PA induced IR in liver cells. These cells were treated with different concentrations of PA for either 8 or 16 h...
February 29, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38401745/in-vitro-test-methods-for-evaluating-high-molecular-weight-polyethylene-oxide-polymer-induced-hemolytic-and-thrombotic-potential
#9
JOURNAL ARTICLE
Dongjune Kim, Rucha Natu, Richard Malinauskas, Jin Hyen Baek, Paul Buehler, Xin Feng, Haiou Qu, Julia Pinto, Xiaoming Xu, Luke Herbertson
To combat opioid abuse, the U.S. Food and Drug Administration (FDA) released a comprehensive action plan to address opioid addiction, abuse, and overdose that included increasing the prevalence of abuse-deterrent formulations (ADFs) in opioid tablets. Polyethylene oxide (PEO) has been widely used as an excipient to deter abuse via nasal insufflation. However, changes in abuse patterns have led to unexpected shifts in abuse from the nasal route to intravenous injection. Case reports identify adverse effects similar to thrombotic thrombocytopenic purpura (TTP) syndrome following the intravenous (IV) abuse of opioids containing PEO excipient...
February 22, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38401744/hsa_circ_0003356-suppresses-gastric-cancer-progression-via-mir-556-5p-fkbp5-axis
#10
JOURNAL ARTICLE
Chuanhui Lu, Jing Wu, Xiaoguang Li, Wei Huang, Yongmu Fang, Ying Huang
BACKGROUND: CircRNAs are implicated in the tumorigenesis of various human cancers. This study aims to explore how circ_0003356 contributes to the development of gastric cancer (GC). METHODS: Circ_0003356 expression was analyzed in GSE184882 dataset and validated in our cohort of GC patients and human GC cell lines. The correlations between circ_0003356 levels and prognostic parameters were analyzed. The contribution of circ_0003356 in GC cell malignant behaviors such as cell survival, apoptosis and invasion were investigated by circ_0003356 overexpression in GC cell lines...
February 22, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38364873/a-comparison-of-dermal-toxicity-models-assessing-suitability-for-safe-r-by-design-decision-making-and-for-screening-nanomaterial-hazards
#11
JOURNAL ARTICLE
Polly McLean, Jessica Marshall, Amaia García, David Beal, Alberto Katsumiti, Marie Carrière, Matthew Boyles
The objective of Safe-by-Design (SbD) is to support the development of safer products and production processes, and enable safe use throughout a materials' life cycle; an intervention at an early stage of innovation can greatly benefit industry by reducing costs associated with the development of products later found to elicit harmful effects. Early hazard screening can support this process, and is needed for all of the expected nanomaterial exposure routes, including inhalation, ingestion and dermal. In this study, we compare in vitro and ex vivo cell models that represent dermal exposures (including HaCaT cells, primary keratinocytes, and reconstructed human epidermis (RhE)), and when possible consider these in the context of regulatory accepted OECD TG for in vitro dermal irritation...
February 14, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38355024/development-of-natural-cosmetic-emulsion-using-the-by-product-of-lecythis-pisonis-seed
#12
JOURNAL ARTICLE
Danielle Braga Portes, Lucas Wetler Abeldt, Cristiane Dos Santos Giubert, Denise Coutinho Endringer, Elisângela Flávia Pimentel
Natural products and their biological activities are currently a subject of great interest to the industrial and scientific sector, due to society's awareness of the proper use of biodiversity and economic and sustainability. To promote the sustainable use of biomass the extract of the by-product of the shell seed of Lecythis pisonis was applied to develop a natural cosmetic emulsion. To ensure safety for its topical use the cytotoxic activity of its crude extract was evaluated by the colorimetric method of 3- bromide (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium, MTT, in cell culture of fibroblasts L929, human keratinocytes HaCat, and human endothelium EA...
February 12, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38355023/gill-epithelial-cell-line-asg-10-from-atlantic-salmon-as-a-new-research-tool-for-solving-water-quality-challenges-in-aquaculture
#13
JOURNAL ARTICLE
Anita Solhaug, Pål A Olsvik, Prabhugouda Siriyappagouder, Randi Faller, Torstein Kristensen
Here we evaluated the gill epithelial cell line ASG-10 from Atlantic salmon, as an in vitro model for research on known water quality challenges in aquaculture. Ammonia/ammonium (NH3 /NH4 + ), a recognized challenge in water-intensive recirculating aquaculture systems (RAS), induced lysosomal vacuolization, reduced protein degradation and cell migration of the ASG-10 cells. Aluminium (Aln+ ), another challenge in freshwater aquaculture facilities had only minor effects. Next, we investigated the tolerance for direct water exposure of ASG-10...
February 12, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38341109/interaction-of-mycotoxins-zearalenone-%C3%AE-zearalenol-and-%C3%AE-zearalenol-with-cytochrome-p450-cyp1a2-2c9-2c19-2d6-and-3a4-enzymes-and-organic-anion-transporting-polypeptides-oatp1a2-oatp1b1-oatp1b3-and-oatp2b1
#14
JOURNAL ARTICLE
Hana Kaci, Ágnes Dombi, Patrik Gömbös, András Szabó, Éva Bakos, Csilla Özvegy-Laczka, Miklós Poór
Zearalenone (ZEN) is a mycoestrogen produced by Fusarium fungi. ZEN is a frequent contaminant in cereal-based products, representing significant health threat. The major reduced metabolites of ZEN are α-zearalenol (α-ZEL) and β-zearalenol (β-ZEL). Since the toxicokinetic interactions of ZEN/ZELs with cytochrome P450 enzymes (CYPs) and organic anion transporting polypeptides (OATPs) have been barely characterized, we examined these interactions applying in vitro models. ZEN and ZELs were relatively strong inhibitors of CYP3A4 and moderate inhibitors of CYP1A2 and CYP2C9...
February 8, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38320684/purinergic-ligands-induce-extracellular-acidification-and-increased-atp-turnover-in-hepg2-cells
#15
JOURNAL ARTICLE
Haotong Chen, Yong Han, Abby Hearne, Anna Monarchino, Jeffrey S Wiseman
Nucleosides and nucleotides at μM concentrations stimulated a 300% increase in acid secretion in HepG2 cells, which was quantitatively accounted for as increased export of lactate generated by glycogenolysis. Agonist selectivity encompassed nucleosides and nucleotides for all 5 natural nucleobases and, along with antagonist profiles, was inconsistent with a role for purinergic receptors in mediating this activity. Agonist catabolism did not contribute significantly to either low selectivity or lactate production...
February 4, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38301920/anti-migratory-and-cytotoxic-effect-of-indole-derivative-in-c6-glioma-cells
#16
JOURNAL ARTICLE
Ana Karolina de Souza Andrade, Mariana Nobre Farias de Franca, Jileno Ferreira Santos, Nathália Araújo Macêdo, Waldecy de Lucca Junior, Ricardo Scher, Socrates Cabral de Holanda Cavalcanti, Cristiane Bani Corrêa
Gliomas are among the most common primary malignant brain tumors. Despite advances in cancer treatment, survival is very low, so the discovery of new therapeutic agents is essential. In this context, indole is an important source for the development of new bioactive molecules. A pharmacological screening of ten indole derivatives was carried out to evaluate the cytotoxic capacity against three tumor cell lines. After pharmacological screening, three compounds were selected, based on their high capacity to reduce cell proliferation, and their IC50 values were determined...
January 30, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38278458/arsenic-trioxide-inhibits-the-response-of-primary-human-b-cells-to-influenza-virus-a-in-vitro
#17
JOURNAL ARTICLE
Luca M Kaiser, Robert A Freeborn, Allison P Boss, Yining Jin, Cheryl E Rockwell
Arsenic compounds are common environmental toxicants worldwide and particularly enriched in the Northeast and the Southwestern United States, the Alps, and Bangladesh. Exposure to arsenic is linked with various detrimental health outcomes, including cancer, cognitive decline, and kidney damage. Our group has previously shown that arsenic trioxides alter T cell cytokine production. In the current study, we demonstrate that exposure to arsenic compounds alters B cell function in an in vitro influenza model. Human peripheral blood mononuclear cells (PBMCs) were isolated from blood and cultured with arsenic trioxide (As3 O2 ) and subsequently challenged with Influenza A virus...
January 24, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38266663/biochanin-a-a-g6pd-inhibitor-in-silico-and-in-vitro-studies-in-non-small-cell-lung-cancer-cells-a549
#18
JOURNAL ARTICLE
Anjali B Thakkar, Ramalingam B Subramanian, Sampark S Thakkar, Vasudev R Thakkar, Parth Thakor
Secondary metabolites from medicinal plants have a well-established therapeutic potential, with many of these chemicals having specialized medical uses. Isoflavonoids, a type of secondary metabolite, have little cytotoxicity against healthy human cells, making them interesting candidates for cancer treatment. Extensive research has been conducted to investigate the chemo-preventive benefits of flavonoids in treating various cancers. Biochanin A (BA), an isoflavonoid abundant in plants such as red clover, soy, peanuts, and chickpeas, was the subject of our present study...
January 22, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38244730/udca-and-18%C3%AE-ga-alleviate-ethinylestradiol-induced-cholestasis-via-downregulating-ror%C3%AE-t-and-cxcr3-signaling-pathway-in-inkt-cells
#19
JOURNAL ARTICLE
Xinyu Li, Xiaojing Liang, Xiaoxia Gu, Mengzhi Zou, Weiping Cao, Chunhui Liu, Xinzhi Wang
Estrogen-induced intrahepatic cholestasis (IHC) is a mild but potentially serious risk and urges for new therapeutic targets and effective treatment. Our previous study demonstrated that RORγt and CXCR3 signaling pathway of invariant natural killer T (iNKT) 17 cells play pathogenic roles in 17α-ethinylestradiol (EE)-induced IHC. Ursodeoxycholic acid (UDCA) and 18β-glycyrrhetinic acid (GA) presents protective effect on IHC partially due to their immunomodulatory properties. Hence in present study, we aim to investigate the effectiveness of UDCA and 18β-GA in vitro and verify the accessibility of the above targets...
January 18, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://read.qxmd.com/read/38242296/pba-alleviates-cadmium-induced-mouse-spermatogonia-apoptosis-by-suppressing-endoplasmic-reticulum-stress
#20
JOURNAL ARTICLE
Rong Wang, Mengyuan Li, Zhen Wu, Wenjing Gong, Mingming Zhang, Yehao Liu, Yuyou Yao, Yanli Ji
OBJECTIVE: Endoplasmic reticulum (ER) stress mediates Cd-caused germ cell apoptosis in testis. The effects of 4-phenylbutyric acid (PBA), a classical chaperone, were investigated on Cd-induced apoptosis in mouse GC-1 spermatogonia cells. METHODS: The cells were pretreated with PBA before Cd exposure. TUNEL and flow cytometry assays were applied to determine apoptosis. Some key biomarkers of ER stress were analyzed using RT-PCR and western blot. RESULTS: as expected, the apoptotic cells exposed to Cd apparently increased...
January 17, 2024: Toxicology in Vitro: An International Journal Published in Association with BIBRA
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