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Toxicology in Vitro: An International Journal Published in Association with BIBRA

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https://www.readbyqxmd.com/read/28526448/the-catechol-o-methyltransferase-inhibitors-tolcapone-and-entacapone-uncouple-and-inhibit-the-mitochondrial-respiratory-chain-in-heparg-cells
#1
David Grünig, Andrea Felser, Jamal Bouitbir, Stephan Krähenbühl
The catechol-O-methyltransferase inhibitor tolcapone causes hepatotoxicity and mitochondrial damage in animal models. We studied the interaction of tolcapone with mitochondrial respiration in comparison to entacapone in different experimental models. In HepaRG cells (human cell-line), tolcapone decreased the ATP content (estimated IC50 100±15μM) and was cytotoxic (estimated IC50 333±45μM), whereas entacapone caused no cytotoxicity and no ATP depletion up to 200μM. Cytochrome P450 induction did not increase the toxicity of the compounds...
May 16, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28511990/corrigendum-to-inhibition-of-autophagy-enhances-hydroquinone-induced-tk6-cell-death-toxicol-in-vitro-41-2017-123-132
#2
Longmei Xu, Jiaxian Liu, Yuting Chen, Lin Yun, Shaoyun Chen, Kairu Zhou, Bei Lai, Li Song, Hui Yang, Hairong Liang, Huanwen Tang
No abstract text is available yet for this article.
May 13, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28511854/acot1-is-a-sensitive-indicator-for-ppar%C3%AE-activation-after-perfluorooctanoic-acid-exposure-in-primary-hepatocytes-of-sprague-dawley-rats
#3
Hui Liu, Jianshe Wang, Nan Sheng, Ruina Cui, Yitao Pan, Jiayin Dai
Perfluorooctanoic acid (PFOA) is one of the most commonly detected and persistent perfluoroalkyl substances (PFASs) found in the environment. We found that cell viability and intracellular oxidant stress increased in primary rat hepatocytes exposed to PFOA (100μM PFOA, 24h), and mitochondrial superoxide increased from 6.25μM PFOA treatment group. To screen for sensitive indicators in mRNA level, we investigated global transcriptome profile alteration after PFOA exposure using RNA-sequencing (RNA-seq) in primary rat hepatocytes, and identified 177 gene transcripts (158 upregulated, 19 downregulated) as significantly changed after exposure to 100μM of PFOA for 24h (fold change ≥2, FDR<0...
May 13, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28506819/prediction-of-delivery-of-organic-aerosols-onto-air-liquid-interface-cells-in-vitro-using-an-electrostatic-precipitator
#4
Zechen Yu, Myoseon Jang, Tara Sabo-Attwood, Sarah E Robinson, Huanhuan Jiang
To better characterize biological responses to atmospheric organic aerosols, the efficient delivery of aerosol to in vitro lung cells is necessary. In this study, chamber generated secondary organic aerosol (SOA) entered the commercialized exposure chamber (CULTEX® Radial Flow System Compact) where it interfaced with an electrostatic precipitator (ESP) (CULTEX® Electrical Deposition Device) and then deposited on a particle collection plate. This plate contained human lung cells (BEAS-2B) that were cultured on a membrane insert to produce an air-liquid interface (ALI)...
May 12, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28506818/measuring-inhibition-of-monoamine-reuptake-transporters-by-new-psychoactive-substances-nps-in-real-time-using-a-high-throughput-fluorescence-based-assay
#5
Anne Zwartsen, Anouk H A Verboven, Regina G D M van Kleef, Fiona M J Wijnolts, Remco H S Westerink, Laura Hondebrink
The prevalence and use of new psychoactive substances (NPS) is increasing and currently over 600 NPS exist. Many illicit drugs and NPS increase brain monoamine levels by inhibition and/or reversal of monoamine reuptake transporters (DAT, NET and SERT). This is often investigated using labor-intensive, radiometric endpoint measurements. We investigated the applicability of a novel and innovative assay that is based on a fluorescent monoamine mimicking substrate. DAT, NET or SERT-expressing human embryonic kidney (HEK293) cells were exposed to common drugs (cocaine, dl-amphetamine or MDMA), NPS (4-fluoroamphetamine, PMMA, α-PVP, 5-APB, 2C-B, 25B-NBOMe, 25I-NBOMe or methoxetamine) or the antidepressant fluoxetine...
May 12, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28506817/in-vitro-cyp-mediated-drug-metabolism-in-the-zebrafish-embryo-using-human-reference-compounds
#6
Moayad Saad, An Matheeussen, Sebastiaan Bijttebier, Evy Verbueken, Casper Pype, Christophe Casteleyn, Chris Van Ginneken, Sandra Apers, Louis Maes, Paul Cos, Steven Van Cruchten
The increasing use of zebrafish embryos as an alternative model for toxicological and pharmacological studies necessitates a better understanding of xenobiotic biotransformation in this species. As cytochrome P450 enzymes (CYPs) play an essential role in this process, in vitro drug metabolism of four human CYP-specific substrates, i.e. dextromethorphan (DXM), diclofenac (DIC), testosterone (TST) and midazolam (MDZ) was investigated in adult male and female zebrafish, and in zebrafish embryos and larvae up to 120hours post-fertilization...
May 12, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28502835/the-adma-ddah-no-pathway-in-human-vein-endothelial-cells-exposed-to-arsenite
#7
Citlalli Osorio-Yáñez, Miguel Chin-Chan, Luz C Sánchez-Peña, Octavio G Atzatzi-Aguilar, Jesus A Olivares-Reyes, José Segovia, Luz M Del Razo
Inorganic arsenic (iAs) exposure is related to cardiovascular disease, which is characterized by endothelial dysfunction and nitric oxide (NO) depletion. The mechanisms underlying NO depletion as related to iAs exposure are not fully understood. The endogenous inhibitor of nitric oxide synthase, asymmetric dimethylarginine (ADMA), might be a molecular target of iAs. ADMA concentrations are regulated by proteins involved in its synthesis (arginine methyl transferase 1 [PRMT-1]) and degradation (dimethylarginine dimethylaminohydrolase [DDAH])...
May 11, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28495400/rapid-breakthrough-of-131-i-in-an-in-vitro-human-epidermis-model
#8
U Nygren, A Hedman, T Nylén, L Thors
Skin contamination with radionuclides may cause local radiation damage, but also systemic distribution if the nuclides penetrate the epidermal membrane. Detailed information of the skin absorption of radionuclides is of importance for e.g. dose estimations and development of decontamination strategies. In the present study, penetration of (131)I through human epidermis was studied using an in vitro flow through diffusion chamber. Epidermis was exposed to a Na(131)I-solution, used in nuclear medicine, and the potential concentration-dependency of skin penetration was examined by including two concentrations of non-radioactive NaI...
May 8, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28495234/oxidative-stress-induced-akt-downregulation-mediates-green-tea-toxicity-towards-prostate-cancer-cells
#9
Anna Maria Posadino, Hoa Thi Phu, Annalisa Cossu, Roberta Giordo, Marco Fois, Duong Thi Bich Thuan, Antonio Piga, Salvatore Sotgia, Angelo Zinellu, Ciriaco Carru, Gianfranco Pintus
Green tea consumption has been shown to possess cancer chemopreventive activity. Polyphenol E (PE) is a widely used standardized green tea extract formulation. This study was designed to investigate the impact of PE on prostate cancer cells (PC3), analyze the potential signals involved and elucidate whether anti- or pro-oxidant effects may be implicated. Treatment of PC3 cells with 30 and 100μg/ml PE significantly decreased cell viability and proliferation. At the tested concentrations, PE did not exert any antioxidant activity, eliciting instead a pro-oxidant effect at concentrations 30 and 100μg/ml, which was consistent with the observed PE cytotoxicity...
May 7, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28483490/nano-sized-iron-particles-may-induce-multiple-pathways-of-cell-death-following-generation-of-mistranscripted-rna-in-human-corneal-epithelial-cells
#10
Eun-Jung Park, Je-Byung Che, Seuyoung Kang, Jungmook Lyu, Uiseok Jeong, Changjoo Yeom, Younghun Kim, Jaerak Chang
Iron is closely associated with an ambient particulate matters-induced inflammatory response, and the cornea that covers the front of the eye, is among tissues exposed directly to ambient particulate matters. Prior to this study, we confirmed that nano-sized iron particles (FeNPs) can penetrate the cornea. Thus, we identified the toxic mechanism of FeNPs using human corneal epithelial cells. At 24h after exposure, FeNPs located inside autophagosome-like vacuoles or freely within human corneal epithelial cells...
May 5, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28483489/multi-walled-carbon-nanotubes-a-cytotoxicity-study-in-relation-to-functionalization-dose-and-dispersion
#11
Lulu Zhou, Henry Jay Forman, Yi Ge, Joseph Lunec
Chemical functionalization broadens carbon nanotube (CNT) applications, conferring new functions, but at the same time potentially altering toxicity. Although considerable experimental data related to CNT toxicity, at the molecular and cellular levels, have been reported, there is very limited information available for the corresponding mechanism involved (e.g. cell apoptosis and genotoxicity). The threshold dose for safe medical application in relation to both pristine and functionalized carbon nanotubes remains ambiguous...
May 5, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28477956/oxidative-damage-and-disturbance-of-antioxidant-capacity-by-zearalenone-and-its-metabolites-in-human-cells
#12
Elena Tatay, Silvia Espín, Antonio-Juan García-Fernández, María-José Ruiz
Mycotoxin contamination of foods and feeds represent a serious problem worldwide. Zearalenone (ZEA) is a secondary metabolite produced by Fusarium species. This study explores oxidative cellular damage and intracellular defense mechanisms (enzymatic and non-enzymatic) in the hepatoma cell line HepG2 after exposure to ZEA and its metabolites (α-zearalenol, α-ZOL; β-zearalenol, β-ZOL). Our results demonstrated that HepG2 cells exposed to ZEA, α-ZOL or β-ZOL at different concentrations (0, 6.25, 12.5 and 25μM) showed: (i) elevated ROS levels (1...
May 3, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28476499/selective-stimulation-of-the-jak-stat-signaling-pathway-by-silica-nanoparticles-in-human-endothelial-cells
#13
S Siegrist, H Kettiger, E Fasler-Kan, J Huwyler
Silica nanoparticles (SiNP) are frequently used in pharmaceutical formulations. Intravenously administered, these particles are in close contact with the vascular endothelium. However, preliminary safety assessments of these novel excipients have indicated that SiNP are potentially cytotoxic and can trigger inflammation. In order to elucidate mechanisms of SiNP mediated inflammation, cerebral microvascular endothelial cells and primary umbilical endothelial cells were incubated with SiNP at doses between 10ng/ml and 250μg/ml...
May 3, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28476498/molecular-targets-of-chloropicrin-in-human-airway-epithelial-cells
#14
Maija Pesonen, Jaana Rysä, Markus Storvik, Kirsi Rilla, Markku Pasanen, Kirsi Vähäkangas
Chloropicrin is a vaporizing, irritating compound that causes complications in the respiratory system when inhaled. In this study, we examined the effects of exposure to chloropicrin for 24h on ultrastructure and global gene expression in primary human bronchial epithelial cells. The treatment increased the number of round and shrunken cells, which detached from culture plates more readily than the untreated control cells. Transmission electron microscopy revealed some swollen mitochondria and the appearance of autophagy/lysosome type of vacuoles in the treated cells...
May 3, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28476500/maintenance-of-high-quality-rat-precision-cut-liver-slices-during-culture-to-study-hepatotoxic-responses-acetaminophen-as-a-model-compound
#15
Anne Granitzny, Jan Knebel, Dirk Schaudien, Armin Braun, Pablo Steinberg, Clemens Dasenbrock, Tanja Hansen
Precision cut liver slices (PCLiS) represent a promising tool in reflecting hepatotoxic responses. However, the culture of PCLiS varies considerably between laboratories, which can affect the performance of the liver slices and thus the experimental outcome. In this study, we describe an easily accessible culture method, which ensures optimal slice viability and functionality, in order to set the basis for reproducible and comparable PCLiS studies. The quality of the incubated rat PCLiS was assessed during a 24h culture period using ten readouts, which covered viability (lactate dehydrogenase-, aspartate transaminase- and glutamate dehydrogenase-leakage, ATP content) and functionality parameters (urea, albumin production) as well as histomorphology and other descriptive characteristics (protein content, wet weight, slice thickness)...
May 2, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28476497/cytotoxic-mechanism-of-baccharis-milleflora-less-dc-essential-oil
#16
Camila Bugnotto Pereira, Carla Cristine Kanunfre, Paulo Vitor Farago, Débora Maria Borsato, Jane Manfron Budel, Beatriz Helena Lameiro de Noronha Sales Maia, Eliane Aparecida Campesatto, Adilson Sartoratto, Marilis Dallarmi Miguel, Obdulio Gomes Miguel
The aim of this study was to investigate the cytotoxic activity of the essential oil from cladodes of Bacharis milleflora in relation to Jurkat, Raji and HL-60 cells, as well as exploring the cell mechanisms in order to elucidate how the cytotoxic process occurs. The presence of the following volatile compounds was detected by GC-MS: bicyclogermacrene (12.16%), germacrene D (11.18%), (E)-caryophyllene (9.28%), and α-humulene (8.05%). In general, IC50 values lower than 50μg/mL were obtained for all the tumor cells at 24, 48 and 72h by MTT assay...
May 2, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28473196/in-vitro-evaluation-of-the-potential-toxic-effects-of-palladium-nanoparticles-on-fibroblasts-and-lung-epithelial-cells
#17
I Iavicoli, M Farina, L Fontana, D Lucchetti, V Leso, C Fanali, V Cufino, A Boninsegna, K Leopold, R Schindl, D Brucker, A Sgambato
Palladium nanoparticles have been increasingly used in catalytic processes, wastewater treatment, electronics, and biomedicine. However, recent evidence proved that these nanoparticles are able to induce adverse effects both in in vitro and in vivo models. Nevertheless, molecular mechanisms underlying the toxic effects are still poorly understood. Therefore, this study aimed to investigate the potential toxicological mechanisms of palladium nanoparticles assessing their effects on normal diploid rat fibroblast and lung carcinoma human epithelial cell lines...
May 1, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28461233/inhibition-of-reductase-systems-by-2-aapa-modulates-peroxiredoxin-oxidation-and-mitochondrial-function-in-a172-glioblastoma-cells
#18
Luiz Felipe de Souza, Ariana Ern Schmitz, Luana Caroline Schüler da Silva, Karen Andrinéia de Oliveira, Cláudia Beatriz Nedel, Carla Inês Tasca, Andreza Fabro de Bem, Marcelo Farina, Alcir Luiz Dafre
Thiol homeostasis has a critical role in the maintenance of proper cellular functions and survival, being coordinated by the action of several reductive enzymes, including glutathione (GSH)/glutathione reductase (GR) and thioredoxin (Trx)/thioredoxin reductase (TrxR) systems. Here, we investigated the effects of the GR inhibitor 2-acetylamino-3-[4-(2-acetylamino-2-carboxyethylsulfanylthiocarbonylamino)phenylthiocarbamoylsulfanyl]propionic acid (2-AAPA) on the activity of thiol reductases (GR and TrxR), redox balance and mitochondrial function of A172 glioblastoma cells...
April 29, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28461234/studies-on-the-contributions-of-smoke-constituents-individually-and-in-mixtures-in-a-range-of-in-vitro-bioactivity-assays
#19
Regina Stabbert, Ruth Dempsey, Joerg Diekmann, Christian Euchenhofer, Timo Hagemeister, Hans-Juergen Haussmann, Arno Knorr, Boris P Mueller, Pavel Pospisil, Wolf Reininghaus, Ewald Roemer, Franz J Tewes, Detlef J Veltel
Tobacco smoke is a complex mixture with over 8700 identified constituents. Smoking causes many diseases including lung cancer, cardiovascular disease, and chronic obstructive pulmonary disease. However, the mechanisms of how cigarette smoke impacts disease initiation or progression are not well understood and individual smoke constituents causing these effects are not generally agreed upon. The studies reported here were part of a series of investigations into the contributions of selected smoke constituents to the biological activity of cigarette smoke...
April 28, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28461232/a-comprehensive-statistical-classifier-of-foci-in-the-cell-transformation-assay-for-carcinogenicity-testing
#20
Giulia Callegaro, Kasja Malkoc, Raffaella Corvi, Chiara Urani, Federico M Stefanini
The identification of the carcinogenic risk of chemicals is currently mainly based on animal studies. The in vitro Cell Transformation Assays (CTAs) are a promising alternative to be considered in an integrated approach. CTAs measure the induction of foci of transformed cells. CTAs model key stages of the in vivo neoplastic process and are able to detect both genotoxic and some non-genotoxic compounds, being the only in vitro method able to deal with the latter. Despite their favorable features, CTAs can be further improved, especially reducing the possible subjectivity arising from the last phase of the protocol, namely visual scoring of foci using coded morphological features...
April 28, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
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