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Toxicology in Vitro: An International Journal Published in Association with BIBRA

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https://www.readbyqxmd.com/read/28232239/novel-na%C3%A3-ve-bayes-classification-models-for-predicting-the-chemical-ames-mutagenicity
#1
Hui Zhang, Yan-Li Kang, Yuan-Yuan Zhu, Kai-Xia Zhao, Jun-Yu Liang, Lan Ding, Teng-Guo Zhang, Ji Zhang
Prediction of drug candidates for mutagenicity is a regulatory requirement since mutagenic compounds could pose a toxic risk to humans. The aim of this investigation was to develop a novel prediction model of mutagenicity by using a naïve Bayes classifier. The established model was validated by the internal 5-fold cross validation and external test sets. For comparison, the recursive partitioning classifier prediction model was also established and other various reported prediction models of mutagenicity were collected...
February 20, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28223142/glucose-capped-silver-nanoparticles-induce-cell-cycle-arrest-in-hela-cells
#2
Elisa Panzarini, Stefania Mariano, Cristian Vergallo, Elisabetta Carata, Gian Maria Fimia, Francesco Mura, Marco Rossi, Viviana Vergaro, Giuseppe Ciccarella, Marco Corazzari, Luciana Dini
This study aims to determine the interaction (uptake and biological effects on cell viability and cell cycle progression) of glucose capped silver nanoparticles (AgNPs-G) on human epithelioid cervix carcinoma (HeLa) cells, in relation to amount, 2×10(3) or 2×10(4) NPs/cell, and exposure time, up to 48h. The spherical and well dispersed AgNPs (30±5nm) were obtained by using glucose as reducing agent in a green synthesis method that ensures to stabilize AgNPs avoiding cytotoxic soluble silver ions Ag(+) release...
February 18, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28223141/activation-of-melatonin-receptor-mt1-2-promotes-p-gp-transporter-in-methamphetamine-induced-toxicity-on-primary-rat-brain-microvascular-endothelial-cells
#3
Pichaya Jumnongprakhon, Sivanan Sivasinprasasn, Piyarat Govitrapong, Chainarong Tocharus, Jiraporn Tocharus
Melatonin has been known as a neuroprotective agent for the central nervous system (CNS) and the blood-brain barrier (BBB), which is the primary structure that comes into contact with several neurotoxins including methamphetamine (METH). Previous studies have reported that the activation of melatonin receptors (MT1/2) by melatonin could protect against METH-induced toxicity in brain endothelial cells via several mechanisms. However, its effects on the P-glycoprotein (P-gp) transporter, the active efflux pump involved in cell homeostasis, are still unclear...
February 18, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28223140/reduced-cell-viability-and-apoptosis-induction-in-human-thyroid-carcinoma-and-mesothelioma-cells-exposed-to-cidofovir
#4
Simona Catalani, Francesco Palma, Serafina Battistelli, Barbara Nuvoli, Rossella Galati, Serena Benedetti
Besides its well-recognized antiviral activity, Cidofovir (CDV) has been shown to exert anticancer properties both within in vitro and in vivo models. The aim of this study was to evaluate the effects of CDV on still unexplored cultured cancer cells from human mesothelioma as well as breast, colon, liver, lung, prostate, and thyroid carcinomas. Overall, a dose- and time-dependent inhibition of cell viability was observed after CDV exposure. To clarify the mechanisms underlying CDV action, apoptotic cell death was investigated in two infected cell lines [Ist-Mes1 and Ist-Mes2 mesothelioma cells (SV40+)] and in two uninfected cell lines (NCI-H2425 mesothelioma cells and FTC-133 thyroid cancer cells), which resulted the most sensitive to CDV treatment...
February 18, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28219724/in-vitro-skin-irritation-assessment-becomes-a-reality-in-china-using-a-reconstructed-human-epidermis-test-method
#5
Nan Li, Yanfeng Liu, Jie Qiu, Lingyan Zhong, Nathalie Alépée, José Cotovio, Zhenzi Cai
The in vitro EpiSkin™ test method was validated in 2007 by the European Union Reference Laboratory for alternatives to animal testing (EURL ECVAM) as a full replacement method for the Draize acute skin irritation test and adopted in the OECD Test Guideline 439 in 2009. Based on the EpiSkin™ technology, the production of a reconstructed epidermis model has been established and standardized in China. The evaluation of the in vitro skin irritation test method using this EpiSkin™ model produced in China was performed on a set of 45 chemicals...
February 17, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28219723/the-protective-effects-of-resveratrol-h2s-and-thermotherapy-on-the-cell-apoptosis-induced-by-cdte-quantum-dots
#6
Tianshu Wu, Qingling Zhan, Ting Zhang, Shengjun Ang, Jiali Ying, Keyu He, Shihan Zhang, Yuying Xue, Meng Tang
Quantum dots (QDs) could be used in the field of biology and medicine as excellent nano-scale fluorescent probes due to their unique optical properties, but the adverse effects of QDs are always the obstruction for its usage in living organisms. In this study, we observed that CdTe QDs exposure decreased the cell viability while increased the apoptosis rates in the L929 cells. Apart from QD-induced oxidative stress indicated by excessive ROS generation, three signal transductions, including Akt, p38 and JNK, played important roles on the regulation of cell apoptosis by CdTe QDs exposure as well...
February 17, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28216176/glutathione-reduces-cytotoxicity-of-polyethyleneimine-coated-magnetic-nanoparticles-in-cho-cells
#7
Klemen Strojan, Jasna Lojk, Vladimir B Bregar, Peter Veranič, Mojca Pavlin
Polyethyleneimine (PEI) is a polycationic compound frequently used as a transfection agent. However, cytotoxicity of PEI and PEI-coated nanoparticles (PEI NPs) is still a major obstacle in its use. In this study we report a method for reducing cytotoxicity of PEI NPs by addition of glutathione in NPs synthesis. Glutathione reduced cytotoxic effects for at least 30% and decreased observed oxidative stress response compared to standard formulation. Results showed that the effect was partially due to reduced zeta potential and partially due to protective antioxidant properties of glutathione...
February 16, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28214551/effect-of-benzalkonium-chloride-on-trabecular-meshwork-cells-in-a-new-in-vitro-3d-trabecular-meshwork-model-for-glaucoma
#8
Meryem Bouchemi, Christophe Roubeix, Karima Kessal, Luisa Riancho, Anne-Laure Raveu, Hayet Soualmia, Christophe Baudouin, Françoise Brignole-Baudouin
PURPOSE: To validate a new culture model of primary human trabecular meshwork cells (p-hTMCs) using Matrigel®, in order to mimic in vitro 3D-TM organization, and to investigate the proinflammatory effect of benzalkonium chloride (BAK) in 3D p-hTMC cultures. METHODS: p-hTMCs, seeded onto Matrigel®-coated inserts were stimulated with BAK (10(-4)%), dexamethasone (DEX) (10(-6)M) or transforming growth factor-beta 2 (TGF-β2) (5ng/ml) for 48h and observed with confocal microscopy...
February 15, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28188836/effect-of-bjcul-a-lectin-isolated-from-bothrops-jararacussu-on-human-peripheral-blood-mononuclear-cells
#9
Weverson Luciano Pires, Onassis Boeri de Castro, Anderson Makoto Kayano, Sulamita da Silva Setúbal, Adriana Silva Pontes, Neriane Monteiro Nery, Mauro Valentino Paloschi, Soraya Dos Santos Pereira, Rodrigo Guerino Stábeli, Carla Freire Celedônio Fernandes, Andreimar Martins Soares, Juliana Pavan Zuliani
BjcuL is a C-type lectin with specificity for the binding of β-d-galactose units isolated from Bothrops jararacussu venom. It triggers cellular infiltration in post capillary venules, increases edema and vascular permeability in murine models, contributes to in vitro neutrophil activation and modulates macrophage functional activation towards an M1 state. The purpose of this study was to investigate the effect of BjcuL on human peripheral blood mononuclear cells (PBMCs) activation with a focus on PBMCs proliferation and inflammatory mediators release...
February 8, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28185889/microrna-hsa-mir-29b-potentiates-etoposide-toxicity-in-hela-cells-via-down-regulation-of-mcl-1
#10
S Kollinerová, Z Dostál, M Modrianský
Etoposide is commonly used as a monotherapy or in combination with other drugs for cancer treatments. In order to increase the drug efficacy, ceaseless search for novel combinations of drugs and supporting molecules is under way. MiRNAs are natural candidates for facilitating drug effect in various cell types. We used several systems to evaluate the effect of miR-29 family on etoposide toxicity in HeLa cells. We show that miR-29b significantly increases etoposide toxicity in HeLa cells. Because Mcl-1 protein has been recognized as a miR-29 family target, we evaluated downregulation of Mcl-1 protein splicing variant expression induced by miR-29 precursors and confirmed a key role of Mcl-1 protein in enhancing etoposide toxicity...
February 6, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28179152/the-six-most-widely-used-selective-serotonin-reuptake-inhibitors-decrease-androgens-and-increase-estrogens-in-the-h295r-cell-line
#11
Cecilie Hurup Hansen, Lizette Weber Larsen, Amalie Møller Sørensen, Bent Halling-Sørensen, Bjarne Styrishave
Selective serotonin reuptake inhibitors (SSRIs) used as first line of treatment in major depressive disorder (MDD) are known to exert negative effects on the endocrine system and fertility. The aim of the present study was to investigate the possible endocrine disrupting effect of six SSRIs, fluoxetine, paroxetine, citalopram and its active enantiomer escitalopram, sertraline and fluvoxamine using the OECD standardized and validated human in vitro adrenocortical H295R cell assay. All the major steroids, including progestagens, corticoids, androgens and estrogens were analysed using a fully validated LC-MS/MS method...
February 5, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28167136/thyroid-hormone-disrupting-potentials-of-bisphenol-a-and-its-analogues-in-vitro-comparison-study-employing-rat-pituitary-gh3-and-thyroid-follicular-frtl-5-cells
#12
Sangwoo Lee, Cheolmin Kim, Hyewon Youn, Kyungho Choi
As adverse health effects of bisphenol A (BPA) become a growing public health concern, the chemicals substituting BPA have been increasingly used in everyday lives. BPA substitutes have been frequently detected in both environment and biota in increasing levels. However, very limited toxicological information is available for these chemicals. In the present study, thyroid disrupting effects of nine structural analogues of BPA were evaluated along with BPA, using rat pituitary (GH3) and thyroid follicular (FRTL-5) cells...
February 5, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28163247/measurement-of-cytotoxicity-and-irritancy-potential-of-sugar-based-surfactants-on-skin-related-3d-models
#13
Biao Lu, Yong Miao, Pascale Vigneron, Vincent Chagnault, Eric Grand, Anne Wadouachi, Denis Postel, Isabelle Pezron, Christophe Egles, Muriel Vayssade
Sugar-based surfactants present surface-active properties and relatively low cytotoxicity. They are often considered as safe alternatives to currently used surfactants in cosmetic industries. In this study, four sugar-based surfactants, each with an eight carbon alkyl chain bound to a glucose or a maltose headgroup through an amide linkage, were synthesized and compared to two standard surfactants. The cytotoxic and irritant effects of surfactants were evaluated using two biologically relevant models: 3D dermal model (mouse fibroblasts embedded in collagen gel) and reconstituted human epidermis (RHE, multi-layered human keratinocytes)...
February 3, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28163246/triphenyl-phosphate-enhances-adipogenic-differentiation-glucose-uptake-and-lipolysis-via-endocrine-and-noradrenergic-mechanisms
#14
German Cano-Sancho, Anna Smith, Michele A La Merrill
The use of triphenyl phosphate (TPhP) as a flame retardant or plasticizer has increased during the last decade, resulting in widespread human exposure without commensurate toxicity assessment. The main objectives of this study were to assess the in vitro effect of TPhP and its metabolite diphenyl phosphate (DPhP) on the adipogenic differentiation of 3T3-L1 cells, as well as glucose uptake and lipolysis in differentiated 3T3-L1 adipocytes. TPhP increased pre-adipocyte proliferation and subsequent adipogenic differentiation of 3T3-L1 cells, coinciding with increased transcription in the CEBP and PPARG pathway...
February 3, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28163245/anti-androgen-2-hydroxyflutamide-modulates-cadherin-catenin-and-androgen-receptor-phosphorylation-in-androgen-sensitive-lncap-and-androgen-independent-pc3-prostate-cancer-cell-lines-acting-via-pi3k-akt-and-mapk-erk1-2-pathways
#15
Ewelina Górowska-Wójtowicz, Anna Hejmej, Alicja Kamińska, Laura Pardyak, Małgorzata Kotula-Balak, Joanna Dulińska-Litewka, Piotr Laidler, Barbara Bilińska
This study aimed to investigate rapid effect of anti-androgen 2-hydroxyflutamide (HF) on cadherin/catenin complex and androgen receptor (AR) phosphorylation in prostate cancer cell lines. In addition, a role of PI3K/Akt and MAPK/ERK1/2 pathways in mediating these effects was explored. We have demonstrated that in androgen-sensitive LNCaP cells HF induced rapid increase of E-cadherin phosphorylation at Ser 838/840 (p<0.05) in MAPK/ERK1/2-dependent manner, whereas phosphorylation of β-catenin at Tyr 654 was unchanged...
February 2, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28161596/polygoni-multiflori-radix-derived-anthraquinones-alter-bile-acid-disposition-in-sandwich-cultured-rat-hepatocytes
#16
Li Kang, Luqin Si, Jing Rao, Dan Li, Ya Wu, Sanlan Wu, Minghui Wu, Sijie He, Wenwen Zhu, Yang Wu, Jiaqiang Xu, Gao Li, Jiangeng Huang
Hepatic adverse reaction associated with Polygoni Multiflori Radix (PMR) has been frequently reported in recent years. Highly-enriched anthraquinones (AQs) in PMR, such as emodin, chrysophanol and physcion, have been found to be hepatotoxic. In the present study, sandwich-cultured rat hepatocytes (SCRHs) were employed to investigate the effect of individual and combined AQs on the disposition of endogenous bile acids (BAs) and exogenous probe substrates including deuterium-labeled taurocholate (d5-TCA), glycochenodeoxycholic acid (d4-GCDCA) and 5 (and 6)-carboxy-2',7'-dichlorofluorescein (CDF)...
February 2, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28137434/copper-mediated-dna-damage-by-the-neurotransmitter-dopamine-and-l-dopa-a-pro-oxidant-mechanism
#17
Nida Rehmani, Atif Zafar, Hussain Arif, Sheikh Mumtaz Hadi, Altaf A Wani
Oxidative DNA damage has been implicated in the pathogenesis of neurological disorders, cancer and ageing. Owing to the established link between labile copper concentrations and neurological diseases, it is critical to explore the interactions of neurotransmitters and drug supplements with copper. Herein, we investigate the pro-oxidant DNA damage induced by the interaction of L-DOPA and dopamine (DA) with copper. The DNA binding affinity order of the compounds has been determined by in silico molecular docking...
January 28, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28131817/tumor-promoting-effects-of-cannabinoid-receptor-type-1-in-human-melanoma-cells
#18
Sara Carpi, Stefano Fogli, Beatrice Polini, Valentina Montagnani, Adriano Podestà, Maria Cristina Breschi, Antonella Romanini, Barbara Stecca, Paola Nieri
The role of endocannabinoid system in melanoma development and progression is actually not fully understood. This study was aimed at clarifying whether cannabinoid-type 1 (CB1) receptor may function as tumor-promoting or -suppressing signal in human cutaneous melanoma. CB1 receptor expression was measured in human melanoma cell lines by real-time PCR. A genetic deletion of CB1 receptors in selected melanoma cells was carried out by using three different short hairpin RNAs (shRNAs). Performance of target gene silencing was verified by real-time PCR and Western blot...
January 26, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28130145/effects-of-chitosan-coated-lipid-core-nanocapsules-on-bovine-sperm-cells
#19
Arthur de Castro Jorge Silva, Mariana Härter Remirão, Caroline Gomes Lucas, William Borges Domingues, Tony Silveira, Julia Dame Paschoal, Denise S Jornada, Carine Dahl Corcine, Antonio Sergio Varela Junior, Willian A Prado, Vinicius Farias Campos, Fabiana Kömmling Seixas, Silvia S Guterres, Adriana R Pohlmann, Tiago Collares
Toxicology studies have a pivotal role for selection of new nanosystems. As lipid-core nanocapsules (LNC) rise as a potential system not only for drug delivery but also for immunotherapy and gene therapy, the demand for models of toxic screening increases, and sperm arises as a promising model due to the easiness to evaluate its viability parameters. LNCs were coated with chitosan, chitosan-coated lipid-core nanocapsules (LNC-CS), in order to modify the nanocapsule surface. We evaluated the toxicity of LNC and LNC-CS after incubation with bovine sperm in different concentrations (2...
January 24, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28126644/evaluation-of-the-potential-of-mobile-phone-specific-electromagnetic-fields-umts-to-produce-micronuclei-in-human-glioblastoma-cell-lines
#20
Halh Al-Serori, Michael Kundi, Franziska Ferk, Miroslav Mišík, Armen Nersesyan, Manuel Murbach, Tamara T Lah, Siegfried Knasmüller
Some epidemiological studies indicate that mobile phones cause glioblastomas in humans. Since it is known that genomic instability plays a key role in the etiology of cancer, we investigated the effects of the universal mobile telecommunications system radiofrequency (UMTS-RF) signal, which is used in "smart" phones, on micronucleus (MN) formation and other anomalies such as nuclear buds (NBUDs) and nucleoplasmatic bridges (NPBs). MN are formed by structural and numerical aberrations, NBs reflect gene amplification and NPBs are formed from dicentric chromosomes...
January 24, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
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