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Toxicology in Vitro: An International Journal Published in Association with BIBRA

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https://www.readbyqxmd.com/read/29775650/formulants-of-glyphosate-based-herbicides-have-more-deleterious-impact-than-glyphosate-on-tm4-sertoli-cells
#1
Alison Vanlaeys, Florine Dubuisson, Gilles-Eric Seralini, Carine Travert
Roundup and Glyphogan are glyphosate-based herbicides containing the same concentration of glyphosate and confidential formulants. Formulants are declared as inert diluents but some are more toxic than glyphosate, such as the family of polyethoxylated alkylamines (POEA). We tested glyphosate alone, glyphosate-based herbicide formulations and POEA on the immature mouse Sertoli cell line (TM4), at concentrations ranging from environmental to agricultural-use levels. Our results show that formulations of glyphosate-based herbicides induce TM4 mitochondrial dysfunction (like glyphosate, but to a lesser extent), disruption of cell detoxification systems, lipid droplet accumulation and mortality at sub-agricultural doses...
May 15, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29772264/reactive-oxygen-species-impair-the-excitation-contraction-coupling-of-papillary-muscles-after-acute-exposure-to-a-high-copper-concentration
#2
Filipe Martinuzo Filetti, Dalton Valentim Vassallo, Mirian Fioresi, Maylla Ronacher Simões
Copper is an essential metal for homeostasis and the functioning of living organisms. We investigated the effects of a high copper concentration on the myocardial mechanics, investigating the reactive oxygen species (ROS) mediated effects. The developed force of papillary muscles was reduced after acute exposure to a high copper concentration and was prevented by co-incubation with tempol, DMSO and catalase. The reuptake of calcium by the sarcoplasmic reticulum was reduced by copper and restored by tempol. The contractile response to Ca2+ was reduced and reversed by antioxidants...
May 14, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29753051/variation-in-doses-and-duration-of-particulate-matter-exposure-in-bronchial-epithelial-cells-results-in-upregulation-of-different-genes-associated-with-airway-disorders
#3
Priya Tripathi, Furong Deng, Anne M Scruggs, Yahong Chen, Steven K Huang
Exposure to particulate matter < 2.5 μm (PM2.5 ) is associated with a variety of airway diseases. Although studies have demonstrated that high doses of PM2.5 cause cytotoxicity and changes to gene expression in bronchial epithelial cells, the effect of lower doses and repeated exposure to PM2.5 are less well studied. Here, we treated BEAS-2B cells with varying doses of PM2.5 for 1-7 days and examined the expression of a variety of genes implicated in airway disorders. At high doses, PM2.5 increased the expression of IL6, TNF, TSLP, CSF2, PTGS2, IL4R, and SPINK5...
May 9, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29751030/high-throughput-toxicity-testing-of-chemicals-and-mixtures-in-organotypic-multi-cellular-cultures-of-primary-human-hepatic-cells
#4
Sophia M Orbach, Marion F Ehrich, Padmavathy Rajagopalan
High-throughput screening (HTS) of liver toxicants can bridge the gap in understanding adverse effects of chemicals on humans. Toxicity testing of mixtures is time consuming and expensive, since the number of possible combinations increases exponentially with the number of chemicals. The combination of organotypic culture models and HTS assays can lead to the rapidly evaluation of chemical toxicity in a cost and time-effective manner while prioritizing chemicals that warrant additional investigation. We describe the design, assembly and toxicant response of multi-cellular hepatic organotypic culture models comprised of primary human or rat cells assembled in 96-well plates (denoted as μOCMs)...
May 8, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29747020/safety-evaluation-of-the-temporary-consolidant-based-on-a-zebrafish-embryo-model
#5
Zhang Lu, Zhang Xiao Yan, Hu Yu Lan, Zhang Bing Jian
Temporary consolidants, such as cyclododecane, menthol, coumarin, and ethyl maltol, are proved to be effective for urgent conservation in archaeological field. However, the safety of these temporary consolidants is still unknown although they are always heavily used in archaeological field. Thus reports on the toxicity of these temporary consolidants are limited. Here, a zebrafish model was used for safety evaluation of these four temporary consolidants. In the present study, four temporary consolidants, including cyclododecane, menthol, coumarin, and ethyl maltol, at different concentrations were incubated with zebrafish embryos, and their biological toxic effects were firstly evaluated...
May 7, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29738787/experimental-challenges-regarding-the-in-vitro-investigation-of-the-nanoparticle-biocorona-in-disease-states
#6
Sherleen Xue-Fu Adamson, Zhoumeng Lin, Ran Chen, Lisa Kobos, Jonathan Shannahan
Toxicological evaluation of nanoparticles (NPs) requires the utilization of in vitro techniques due to their number and diverse properties. Cell culture systems are often lacking in their ability to perform comparative toxicity assessment due to dosimetry issues and capacity to simulate in vivo environments. Upon encountering a physiological environment, NPs become coated with biomolecules forming a biocorona (BC), influencing function, biodistribution, and toxicity. Disease-induced alterations in the biological milieu can alter BC formation...
May 5, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29733892/suppression-of-t-lymphocyte-activation-by-3-chloro-1-2-propanediol-mono-and-di-palmitate-esters-in-vitro
#7
Guoren Huang, Jinli Xue, Xiangjun Sun, Jing Wang, Yilin Wang, Thomas T Y Wang, LucyYu Liangli
This study investigated whether and how 3-chloro-1,2-propanediol (3-MCPD) fatty acid esters, a group of food contaminants formed during processing, might inhibit the immune system through suppressing T lymphocyte activation for the first time. Three 3-MCPD esters including 1-palmitoyl-3-chloropropanediol (1-pal), 2-palmitoyl-3-chloropropanediol (2-pal), and1,2-dipalmitoyl-3-chloropropanediol (dipal) were selected as the probe compounds to test the possible effects of fatty acid structure on their potential immune inhibitory effect...
May 4, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29730415/anticancer-activity-of-flavonoids-isolated-from-achyrocline-satureioides-in-gliomas-cell-lines
#8
Priscila Oliveira de Souza, Sara Elis Bianchi, Fabrício Figueiró, Luana Heimfarth, Karla Suzana Moresco, Rosângela Mayer Gonçalves, Juliana Bender Hoppe, Caroline Peres Klein, Christianne Gazzana Salbego, Daniel Pens Gelain, Valquíria Linck Bassani, Alfeu Zanotto Filho, José Claudio Fonseca Moreira
Achyrocline satureioides, popularly known as "marcela", is a medicinal plant found in South America. This plant is rich in flavonoids, which have been reported to exert numerous biological activities. The aim of this study was to purify, identify and evaluate the mechanisms underlining anticancer activity of A. satureioides flavonoids in glioma cell lines (U87, U251 and C6) as well as their comparative toxicity in normal brain cells (primary astrocytes, neurons and organotypic hippocampal cultures)...
May 3, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29729358/doxorubicin-induced-mitophagy-and-mitochondrial-damage-is-associated-with-dysregulation-of-the-pink1-parkin-pathway
#9
Jian Yin, Jiabin Guo, Qiang Zhang, Lan Cui, Li Zhang, Tingfen Zhang, Jun Zhao, Jin Li, Alistair Middleton, Paul L Carmichael, Shuangqing Peng
The usefulness of doxorubicin (DOX), a potent anticancer agent, is limited by its cardiotoxicity. Mitochondria play a central role in DOX-induced cardiotoxicity though the precise mechanisms are still obscure. Increasing evidence indicates that excessive activation of mitophagy and mitochondrial dysfunction are key causal events leading to DOX-induced cardiac injury. The PINK1/parkin pathway has emerged as a critical pathway in regulation of mitophagy as well as mitochondrial function. The present study was aimed to investigate the role of PINK1/parkin pathway in DOX-induced mitochondrial damage and cardiotoxicity...
May 3, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29723632/phenotypic-markers-of-oral-keratinocytes-seeded-on-two-distinct-3d-oral-mucosa-models
#10
Fernanda G Basso, Taisa N Pansani, Cyntia L Marcelo, Carlos Alberto de Souza Costa, Josimeri Hebling, Stephen E Feinberg
This study validates the use of a full-thickness oral mucosa model for in vitro studies with a collagen type I matrix, by comparison of this model with two other 3D oral mucosa models: human-sourced and porcine acellular dermal matrices (AlloDerm®/Strattice®, respectively). For the collagen matrix model, gingival fibroblasts were seeded either onto the dermal side of the AlloDerm® and Strattice® matrices or within the collagen matrices in complete culture medium (DMEM). For all scaffolds, DMEM was replaced every 24 h up to 72 h...
April 30, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29723631/paraptosis-in-human-glioblastoma-cell-line-induced-by-curcumin
#11
Monika Garrido-Armas, Juan Carlos Corona, Maria Luisa Escobar, Leda Torres, Francisco Ordóñez-Romero, Abrahan Hernández-Hernández, Francisco Arenas-Huertero
Curcumin is a polyphenol compound extracted from Curcuma longa plant, is a molecule with pleiotropic effects that suppresses transformation, proliferation and metastasis of malignant tumors. Curcumin can cause different kinds of cell death depending of its concentration on the exposed cell type. Here we show that exposure of the glioblastoma cell line A172 to curcumin at 50 μM, the IC50, causes morphological changes characteristic of paraptosis cell-death. Vesicles derived from the ER and low membrane potential of the mitochondria were constantly found in the exposed cells...
April 30, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29715505/the-exceptional-sensitivity-of-brain-mitochondria-to-copper
#12
Sabine Borchard, Francesca Bork, Tamara Rieder, Carola Eberhagen, Bastian Popper, Josef Lichtmannegger, Sabine Schmitt, Jerzy Adamski, Martin Klingenspor, Karl-Heinz Weiss, Hans Zischka
Wilson disease (WD) is characterized by a disrupted copper homeostasis resulting in dramatically increased copper levels, mainly in liver and brain. While copper damage to mitochondria is an established feature in WD livers, much less is known about such detrimental copper effects in other organs. We therefore assessed the mitochondrial sensitivity to copper in a tissue specific manner, namely of isolated rat liver, kidney, heart, and brain mitochondria. Brain mitochondria presented with exceptional copper sensitivity, as evidenced by a comparatively early membrane potential loss, profound structural changes already at low copper dose, and a dose-dependent reduced capacity to produce ATP...
April 28, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29709566/organic-components-of-airborne-dust-influence-the-magnitude-and-kinetics-of-dendritic-cell-activation
#13
Magali Boucher, Pascale Blais-Lecours, Valérie Létourneau, Marc Veillette, Caroline Duchaine, David Marsolais
Bioaerosol exposure in highly contaminated occupational settings is associated with an increased risk of disease. Yet, few determinants allow for accurate prediction of the immunopathogenic potential of complex bioaerosols. Since dendritic cells are instrumental to the initiation of immunopathological reactions, we studied how dendritic cell activation was modified in response to individual agents, combined microbial agents, or air sample eluates from highly contaminated environmental settings. We found that combinations of agents accelerated and enhanced the activation of in vitro-generated murine bone marrow-derived dendritic cell cultures, when compared to individual agents...
April 27, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29698667/transepidermal-water-loss-and-skin-conductance-as-barrier-integrity-tests
#14
Qian Zhang, Michael Murawsky, Terri LaCount, Gerald B Kasting, S Kevin Li
In vitro skin permeation studies are commonly used in the risk assessment of toxic compound skin exposure. The present study examined the utility of transepidermal water loss (TEWL) and electrical conductance as barrier integrity tests before skin permeation studies in vitro using a large number of skin samples and fentanyl. TEWL and conductance of the skin samples were measured before the permeation experiments in Franz diffusion cells in vitro with a vapometer and low voltage application, respectively. The data were analyzed based on the in vitro permeation results and in vivo skin absorption information from the transdermal fentanyl product labels...
April 23, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29698666/ovatodiolide-suppresses-yes-associated-protein-1-modulated-cancer-stem-cell-phenotypes-in-highly-malignant-hepatocellular-carcinoma-and-sensitizes-cancer-cells-to-chemotherapy-in-vitro
#15
Hang-Lung Chang, Hsin-An Chen, Oluwaseun Adebayo Bamodu, Kwai-Fong Lee, Yew-Min Tzeng, Wei-Hwa Lee, Jo-Ting Tsai
The cancer stem cells (CSCs) theory recently became a focus of heightened attention in cancer biology, with the proposition that CSCs may constitute an important therapeutic target for effective anticancer therapy, because of their demonstrated role in tumor initiation, chemo-, and radio-resistance. Liver CSCs are a small subpopulation of poorly- or undifferentiated liver tumor cells, implicated in tumorigenesis, metastasis, resistance to therapy and disease relapse, enriched with and associated with the functional markers corresponding to the CSCs-enriched side population (SP), high aldehyde dehydrogenase (ALDH) activity, and enhanced formation of in vitro liver CSCs models, referred to herein as hepatospheres...
April 23, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29678786/fetal-bovine-serum-induces-sustained-but-reversible-epithelial-mesenchymal-transition-in-the-beas-2b-cell-line
#16
S W Malm, E A Amouzougan, W T Klimecki
BEAS-2B is a non-malignant, immortalized human cell line that has been used extensively as a model of lung epithelium. Despite ATCC recommendations to culture BEAS-2B in defined, serum-free media, many publications describe culturing BEAS-2B in fetal bovine serum (FBS)-containing media. The objective of this study was to define the effects of FBS on BEAS-2B cells. FBS exposure resulted in increased nuclear levels of transcription factors responsible for regulating epithelial-mesenchymal transition (EMT), increased cell invasiveness and increased anchorage-independent growth...
April 17, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29673971/changes-in-trophoblasts-gene-expression-in-response-to-perchlorate-exposition
#17
De la Peña Sol, Santiago-Roque Isela, Olivo-Vidal Zendy, Navarro-Meza Mónica, Xochihua-Rosas Irene, Arroyo-Helguera Omar
Contaminated water with chlorates is a public health problem associated with iodine deficiency. Epidemiological evidence shows that iodine deficiency is a risk factor for preeclampsia (PE). In this study we use human BeWo trophoblast cells exposed to perchlorate (KClO4 ) and changes in gene expression were analyzed by microarrays, quantitative RT-PCR (qRT-PCR) and immunoblot. The microarray analysis identified 48 transcripts up-regulated and 112 down-regulated in comparison with non-exposed trophoblast. The qRT-PCR analysis confirmed changes in GAS7, PKP2, Emilin, Dynatic 3, protocadherins 11, 15, gamma A12, EGFR, SAFB1, ACE2, ANXA2, Apoliprotein E, SREBF1, and C/EBP-β...
April 16, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29665408/neuroprotective-effect-of-angiotensin-1-7-against-rotenone-induced-oxidative-damage-in-cath-a-neurons
#18
Yong Zhou, Min Li, Dong-Lin Zhu, Teng Jiang, Qing Gao, Xiao-Hong Tang, Shu-Gang Zhang, Jie Lu, Ying-Dong Zhang
There is evidence to support that ROSs are increased in Parkinson's disease (PD). Our recent research showed that angiotensin II (Ang II) participated in the pathogenesis of PD by triggering oxidative stress. Angiotensin-(1-7)[Ang-(1-7)] has been shown to moderate the adverse effects of the Ang II in many diseases. The purpose of the present study was to determine whether the Ang-(1-7) could have similar effects in CATH.a neurons. We used rotenone-induced neuron injury models to evaluate changes in cultured CATH...
April 14, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29665407/3-4-dihydroxybenzaldehyde-quenches-ros-and-rns-and-protects-human-blood-cells-from-cr-vi-induced-cytotoxicity-and-genotoxicity
#19
Nazim Husain, Riaz Mahmood
Hexavalent chromium [Cr(VI)] is the most toxic and potent form of chromium and induces multiple organ damage in humans and experimental animals. Oxidative stress has been implicated in the toxicity of Cr(VI). We have examined the potential role of 3,4-dihydroxybenzaldehyde (DHB), a plant polyphenolic antioxidant, in protecting human erythrocytes and lymphocytes from Cr(VI)-induced cytotoxicity and genotoxicity. Erythrocytes were treated with potassium dichromate, a Cr(VI) compound, in presence and absence of DHB...
April 14, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29660445/development-of-a-new-tool-for-the-long-term-in-vitro-ecotoxicity-testing-of-nanomaterials-using-a-rainbow-trout-cell-line-rtl-w1
#20
L Galbis-Martínez, M L Fernández-Cruz, L Alte, A Valdehita, I Rucandio, J M Navas
The current wide use of manufactured nanomaterials (MNs) is leading to the release of nanoparticles (NPs) to water bodies. Aquatic organisms, including fish, are exposed to low concentrations of NPs for long periods of time being necessary to develop laboratory toxicity tests reflecting realistic conditions. Additionally, today there is a demand of in vitro assays respecting the 3Rs principle. Thus, the main aim of this work was to stablish an in vitro tool for the assessment of long-term NPs ecotoxicity. Considering the key role of liver in detoxification, a rainbow trout liver cell line, RTL-W1, was used...
April 13, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
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