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Molecular and Cellular Probes

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https://www.readbyqxmd.com/read/28627450/impact-of-fixation-artifacts-and-threshold-selection-on-high-resolution-melting-analysis-for-kras-mutation-screening
#1
Wendy Pérez-Báez, Ethel A Garcia-Latorre, Hector Aquiles Maldonado-Martinez, Iris Coronado-Martinez, Leonardo Flores-Garcia, Lucía Taja-Chayeb
INTRODUCTION: Treatment in the metastatic colorectal cancer has expanded with monoclonal antibodies targeting Epidermal Growth Factor Receptor, but is restricted to patients with a wild-type (WT) KRAS mutational status. The most sensitive assays for KRAS mutation detection in formalin-fixed paraffin embedded (FFPE) tissues are based on Real-time PCR. Among them, high resolution melting analysis (HRMA), is a simple, fast, highly sensitive, specific and cost-effective method, proposed as adjunct for KRAS mutation detection...
June 13, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28627449/microrna-214-regulates-immunity-related-genes-in-bovine-mammary-epithelial-cells-by-targeting-nfatc3-and-traf3
#2
Ning Song, Xingping Wang, Linsheng Gui, Sayed Haidar Abbas Raza, Zhuoma Luoreng, Linsen Zan
In human, microRNA-214 (miR-214) plays crucial roles in mechanisms of immunity. However, the potential importance of miR-214 in immune mechanisms in dairy cows has not been investigated. In this study, we assessed potential immunity-related functions of miR-214 in human 293A cells and in bovine mammary epithelial cells (BMECs). We found that NFATc3 and TRAF3 could be targeted by miR-214 in both 293A cells and BMECs. We also found that miR-214 indirectly inhibited the expression of MAP3K14, TBK1 and inflammatory cytokines IL-6 and IL-1β...
June 13, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28587995/duffy-blood-group-system-new-genotyping-method-and-distribution-in-a-brazilian-extra-amazonian-population
#3
Marina Lobato Martins, Adão Rogerio da Silva, Hadassa Campos Santos, Michelle Teodoro Alves, Luciana Cayres Schmidt, Stela Brener Vertchenko, Luci Maria SantAna Dusse, Maria Clara Fernandes da Silva Malta
Duffy blood group system is of interest in several fields of science including transfusion medicine, immunology and malariology. Although some methods have been developed for Duffy polymorphism genotyping, not all of them have been sufficiently described and validated, and all present limitations. At the same time, the frequency of Duffy alleles and antigens in some densely populated regions of the world are still missing. In this study we present new tests for genotyping the major alleles of the Duffy blood system and describe Duffy alleles and antigens in blood donors and transfusion-dependent patients in Minas Gerais, Brazil...
June 3, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28578011/homology-modeling-and-in-silico-prediction-of-ulcerative-colitis-associated-polymorphisms-of-nod1
#4
Ishani Majumdar, Isha Nagpal, Jaishree Paul
Cytosolic pattern recognition receptors play key roles in innate immune response. Nucleotide binding and oligomerisation domain containing protein 1 (NOD1) belonging to the Nod-like receptor C (NLRC) sub-family of Nod-like receptors (NLRs) is important for detection and clearance of intra-cellular Gram negative bacteria. NOD1 is involved in activation of pro-inflammatory pathways. Limited structural data is available for NOD1. Using different templates for each domain of NOD1, we determined the full-length homology model of NOD1...
May 31, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28558918/general-phytoplasma-detection-by-a-q-pcr-method-using-mycoplasma-primers
#5
Eleonora Satta, Irene M Nanni, Nicoletta Contaldo, Marina Collina, José B Poveda, Ana S Ramírez, Assunta Bertaccini
Phytoplasmas and mycoplasmas are bacteria belonging to the class Mollicutes. In this study, a fine tuning of quantitative polymerase chain reaction (qPCR) with a universal mycoplasma primer pair (GPO3F/MGSO) targeting the 16S rRNA gene was carried out on phytoplasmas. The dissociation curves of DNAs from Catharanthus roseus phytoplasma-infected micropropagated shoots and from phytoplasma field-infected plant samples showed a single peak at 82.5 °C (±0.5) specifically detecting phytoplasmas belonging to several ribosomal groups...
May 27, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28552829/identification-of-male-specific-aflp-and-scar-markers-in-the-dioecious-plant-humulus-scandens
#6
Shu-Fen Li, Lian-Jun Wang, Chuan-Liang Deng, Wu-Jun Gao
In this study, 17 male-specific amplified fragment length polymorphism (AFLP) markers were identified between male and female Humulus scandens plants. BLAST analysis revealed that 7 of the 17 sex-linked sequences were highly similar to retrotransposons. Two stable male-specific sequence-characterized amplified regions (SCAR) markers were developed. These AFLP and SCAR markers are novel molecular probes that can be used efficiently to identify the genetic gender of H. scandens and may provide a basis for further investigations on the evolution of sex chromosomes...
May 25, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28546132/identification-of-direct-target-genes-of-mir-7-mir-9-mir-96-and-mir-182-in-the-human-breast-cancer-cell-lines-mcf-7-and-mda-mb-231
#7
Hamidreza Moazzeni, Ali Najafi, Marzieh Khani
Some microRNAs have carcinogenic or tumor suppressive effects in breast cancer, which is the most common cancer in women worldwide. MiR-7 and miR-9 are tumor suppressor microRNAs, which induce apoptosis and inhibit proliferation in breast cancer cells. Moreover, miR-96 and miR-182 are onco-microRNAs that increase proliferation, migration, and tumorigenesis in breast cancer cells. This study aimed to identify the direct target genes of these four microRNAs in the human breast cancer cell lines MCF-7 and MDA-MB-231...
May 22, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28526589/a-rapid-salmonella-detection-method-involving-thermophilic-helicase-dependent-amplification-and-a-lateral-flow-assay
#8
Xin-Jun Du, Tian-Jiao Zhou, Ping Li, Shuo Wang
Salmonella is a major foodborne pathogen that is widespread in the environment and can cause serious human and animal disease. Since conventional culture methods to detect Salmonella are time-consuming and laborious, rapid and accurate techniques to detect this pathogen are critically important for food safety and diagnosing foodborne illness. In this study, we developed a rapid, simple and portable Salmonella detection strategy that combines thermophilic helicase-dependent amplification (tHDA) with a lateral flow assay to provide a detection result based on visual signals within 90 min...
May 16, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28502787/no-association-detected-between-seven-common-variants-in-the-cdkal1-gene-and-gestational-glycemic-traits
#9
Hongfang Ju, Rong Lin, Ziyu Yuan, Yuantian Sun, Liangliang Zeng, Zhenyu Su, Li Jin
Elevated plasma glucose levels in pregnancy increase adverse pregnancy outcomes. Cyclin-dependent kinase 5 regulatory subunit-associated protein 1-like 1 (CDKAL1) has been shown to be involved in insulin secretion in pancreatic β cells. In this study, we investigated the impact of genetic variants in CDKAL1 on plasma glucose, insulin values, β cell function and insulin resistance in the fasted state as well as plasma glucose 1 h after the consumption of a 50-g oral glucose load between 24 and 28 weeks of pregnancy among 929 unrelated pregnant Han Chinese women...
May 11, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28499542/development-of-a-taqman-based-real-time-pcr-assay-for-the-rapid-and-specific-detection-of-novel-duck-origin-goose-parvovirus
#10
Jianchang Wang, Jinfeng Wang, Yuan Cui, Huizhu Nan, Wanzhe Yuan
A real-time PCR assay was developed for specific detection of novel duck-origin goose parvovirus (N-GPV), the etiological agent of duck beak atrophy and dwarfism syndrome (BADS). The detection limit of the assay was 10(2) copies. The assay was useful in the prevention and control of BADS.
May 9, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28499541/sequence-analysis-of-haemagglutinin-and-neuraminidase-of-h1n1-strain-from-a-patient-coinfected-with-mycobacterium-tuberculosis
#11
Ahmed N Alghamdi, Mohammad E Mahfouz, Fahd A Hamdi, Daifullah Al Aboud, Tawfiq Z Al-Laylah, Mohammed I Alotaibi, Khalid W A Al-Thomali, Ahmed S Abdel-Moneim
The 2009 H1N1 pandemic (H1N1pdm09) was associated with a considerable influenza-related morbidity and mortality. Among the complications, Mycobacterial tuberculosis was recorded as a coinfection with influenza in rare cases. The full-length sequences of the viral haemagglutinin and neuraminidase of H1N1pdm09 influenza A virus were analyzed from a recently infected patient. The patient was chronically infected with Mycobacterium tuberculosis. Molecular modelling and in-silico docking of the virus, and other selected strains with the drug oseltamivir were conducted and compared...
May 9, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28461258/thiolated-aunp-probes-and-multiplex-pcr-for-molecular-detection-of-staphylococcus-epidermidis
#12
Mahsa Osmani Bojd, Hossein Kamaladini, Fatemeh Haddadi, Akbar Vaseghi
The emergence of nanotechnology in biology helps to apply the gold nanoparticle probes for fast and accurate identification of pathogens compared to the time-consuming and non-precise phenotypic methods. In this study, two molecular methods have been established for the accurate identification of staphylococcus epidermidis from other coagulase-negative staphylococci. Multiplex PCR was performed using designed primers for Gmk2 and pta housekeeping genes, and SESB specific gene of S. epidermidis. Colorimetric detection by gold nanoparticle probes was carried out using two 20-base thiolated probes designed based on the sequence of pta housekeeping gene of S...
April 28, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28457983/assessment-of-ns1-gene-specific-real-time-quantitative-taqman-pcr-for-the-detection-of-human-bocavirus-in-respiratory-samples
#13
Marco Ligozzi, Erica Diani, Federica Lissandrini, Rosanna Mainardi, Davide Gibellini
Human Bocaviruses (HBoV) were associated with respiratory diseases. Here, we assessed a TaqMan(®)-based PCR for the detection of all four HBoV subtype infections with a sensitivity up to 15 copies/reaction. To evaluate this assay on clinical samples, 178 nasopharyngeal aspirate specimens from pediatric cases were analyzed and HBoV genome was detected in 13 out of 178 patients with a viral load range between 1.6 × 10(3) and 9.4 × 10(7) copies/ml. These results indicated that this method could be used as an alternative technique for the diagnosis of HBoV infection...
April 27, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28455235/computational-prediction-and-analysis-of-deleterious-cancer-associated-missense-mutations-in-dync1h1
#14
Ceren Sucularli, Melda Arslantas
Dynein, cytoplasmic 1, heavy chain 1 (DYNC1H1) gene encodes a subunit of human cytoplasmic dynein complex, which has several crucial functions in the cell, such as intracellular transport of DNA damage proteins and mitotic spindle positioning. Recent studies reported the altered expression of DYNC1H1 in different cancers and DYNC1H1 was suggested to be potential biomarker in colorectal cancers. Previously, DYNC1H1 mutations have been associated with neurodegenerative diseases, however mutations of DYNC1H1 have not been fully investigated in cancers except for different types of pancreatic cancers...
April 25, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28433811/rapid-and-sensitive-detection-of-salmonid-alphavirus-using-taqman-real-time-pcr
#15
Wen Shi, Aochen Song, Shuai Gao, Yuting Wang, Lijie Tang, Yigang Xu, Tong Ren, Yijing Li, Min Liu
Salmonid alphavirus (SAV) infection has led to the spread of salmon pancreas disease (PD) and sleeping disease (SD) to salmonids in several countries in Europe, resulting in tremendous economic losses to the fish farming industry. Recently, with increases in the fish import trade, many countries in which SAV has been unreported, such as China, may be seriously threatened by these diseases. It is therefore necessary to develop efficient detection methods for the prevention and diagnosis of SAV infection. In this study, a rapid and sensitive TaqMan real-time PCR method was established and assessed for this purpose...
April 19, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28400333/umbilical-cord-mesenchymal-stem-cells-are-able-to-undergo-differentiation-into-functional-islet-like-cells-in-type-2-diabetic-tree-shrews
#16
Xing-Hua Pan, Xia Huang, Guang-Ping Ruan, Rong-Qing Pang, Qiang Chen, Jin-Xiang Wang, Jie He, Jing Zhao, Xue-Min Cai, Na Zhao, Yi Chen, Xiang-Qing Zhu
Islet transplantation is arguably one of the most promising strategies to treat patients suffering with diabetes mellitus. However, a combination of a lack of donors and chronic immune rejection limit clinical applications. Here, we evaluated the efficacy of cell therapy using islet-like cells differentiated from umbilical cord mesenchymal stem cells (UC-MSCs) of tree shrews for the treatment of type 2 diabetes. Enhanced green fluorescent protein (eGFP) labeled UC-MSCs were directly injected into type 2 diabetic tree shrews, where UC-MSC differentiated into functional islet-like cells and alleviated disease severity, as evidenced by improved biochemical features and reduced concentrations of inflammatory cytokines...
April 8, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28576288/editor-note
#17
EDITORIAL
(no author information available yet)
No abstract text is available yet for this article.
June 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28411089/evidence-for-polymicrobial-communities-in-explanted-vascular-filters-and-atheroma-debris
#18
Jeremy E Ellis, Richard Heuser, Dara S Missan, Delyn Martinez, Avory Heningburg, Matthew Shabilla, Renata Schwartz, Stephen Fry
RATIONALE: Microbial communities have been implicated in a variety of disease processes and have been intermittently observed in arterial disease; however, no comprehensive unbiased community analysis has been performed. We hypothesize that complex microbial communities may be involved in chronic vascular diseases as well and may be effectively characterized by molecular assays. OBJECTIVE: The main objective is to survey vascular debris, atheroma, and vascular filters for polymicrobial communities consisting of prokaryotic and eukaryotic microbes, specifically eukaryotic microbes...
June 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28342800/development-of-an-isothermal-recombinase-polymerase-amplification-assay-for-rapid-detection-of-pseudorabies-virus
#19
Yang Yang, Xiaodong Qin, Wei Zhang, Zhiyong Li, Shuaijun Zhang, Yanmin Li, Zhidong Zhang
Recombinase polymerase amplification assays using real-time fluorescent detection (real-time RPA assay) and lateral flow dipstick (RPA LFD assay) were developed targeting the gD gene of pseudorabies virus (PRV). Both assays were performed at 39 °C within 20 min. The sensitivity of the real-time RPA assay and the RPA LFD assay was 100 copies per reaction and 160 copies per reaction, respectively. Both assays did not detect DNAs from other virus or PRV negative samples. Therefore, the developed RPA assays provide a rapid, simple, sensitive and specific alternative tool for detection of PRV...
June 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28336361/a-novel-multiplex-pcr-assay-for-simultaneous-detection-of-nine-clinically-significant-bacterial-pathogens-associated-with-bovine-mastitis
#20
Aqeela Ashraf, Muhammad Imran, Tahir Yaqub, Muhammad Tayyab, Wasim Shehzad, Peter C Thomson
For rapid and simultaneous detection of nine bovine mastitic pathogens, a sensitive and specific multiplex PCR assay was developed. The assay was standardized using reference strains and validated on mastitic milk cultures which were identified to species level based on 16S rRNA sequencing. Multiplex PCR assay also efficiently detected the target bacterial strains directly from milk. The detection limit of the assay was up to 50 pg for DNA isolated from pure cultures and 10(4) CFU/ml for spiked milk samples...
June 2017: Molecular and Cellular Probes
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