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Molecular and Cellular Probes

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https://www.readbyqxmd.com/read/28013043/a-field-evaluation-of-an-isothermal-dna-amplification-assay-for-the-detection-of-theileria-annulata-infection-in-cattle
#1
Jacinto Gomes, Marcos Santos, Ana Amaro, Isabel Pereira da Fonseca, Gabriela Santos-Gomes, João Inácio
A loop-mediated isothermal amplification (LAMP) assay was evaluated for the detection of Theileria annulata infection in cattle. The results were compared with a real-time PCR used for the quantification of T. annulata parasitaemia. One hundred bovine blood samples from 16 cattle farms were tested with LAMP and real-time PCR, with T. annulata DNA being detected in 66% and 67% of the samples, respectively. The results showed that the LAMP assay detects a parasitaemia as low as 0.00025%, indicating a high analytical sensitivity of LAMP for clinical diagnosis of bovine theileriosis...
December 22, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28013042/production-of-stable-gfp-expressing-neural-cells-from-p19-embryonal-carcinoma-stem-cells
#2
Hedayatollah Shirzad, Fariba Esmaeili, Shabnam Bakhshalizadeh, Marzieh Ebrahimie, Esmaeil Ebrahimie
Murine P19 embryonal carcinoma (EC) cells are convenient to differentiate into all germ layer derivatives. One of the advantages of P19 cells is that the exogenous DNA can be easily inserted into them. Here, at the first part of this study, we generated stable GFP-expressing P19 cells (P19-GFP(+)). FACS and western-blot analysis confirmed stable expression of GFP in the cells. We previously demonstrated the efficient induction of neuronal differentiation from mouse ES and EC cells by application of a neuroprotective drug, selegiline In the second part of this study selegiline was used to induce differentiation of P19-GFP(+) into stable GFP-expressing neuron-like cells...
December 21, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28011345/genotyping-of-friesian-horses-to-detect-a-hydrocephalus-associated-c-1423c-t-mutation-in-b3galnt2-using-pcr-rflp-and-pcr-pira-methods-frequency-in-stallion-horses-in-m%C3%A3-xico
#3
Miguel Angel Ayala-Valdovinos, Jorge Galindo-García, David Sánchez-Chiprés, Theodor Duifhuis-Rivera
Hydrocephalus in Friesian horses is an autosomal recessive hereditary disease that can result in an abortion, a stillbirth, or euthanization of a newborn foal. Here, the hydrocephalus-associated c.1423C > T mutation in B3GALNT2 gene was detected with PCR-RFLP and PCR-PIRA methods for horse genotyping. A preliminary genotyping survey was performed on 83 randomly selected Friesian stallion horses to determine the current allele frequency in Mexico. The frequency of the mutant T allele was 9.6%.
December 21, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27989424/designing-and-computational-validation-of-microarray-probe-sets-for-the-diagnosis-of-viruses-of-non-human-vertebrates
#4
Gaurava K Rai, Rubina Lawrence
Making specific oligonucleotide probes for diagnosis and differentiation of viruses has always been a challenging task due to unavailability of defined viral sequence databases, probe designing softwares and higher degree of sequence homology among the types and subtypes of viruses. We have provided here, a streamlined procedure to design microarray probes involving various computational tools which is capable of yielding highly specific as well as conserved oligonucleotides to facilitate unbiased diagnosis of viruses...
December 15, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27986555/serological-approaches-for-the-diagnosis-of-schistosomiasis-a-review
#5
Rebecca Hinz, Norbert Georg Schwarz, Andreas Hahn, Hagen Frickmann
Schistosomiasis is a common disease in endemic areas of Sub-Saharan Africa, South America and Asia. It is rare in Europe, mainly imported from endemic countries due to travelling or immigration. Available methods for the diagnosis of schistosomiasis comprise microscopical, molecular and serological approaches, with the latter detecting antigens or antibodies associated with Schistosoma spp. infection. The serological approach is a valuable screening tool in low-endemicity settings and for travel medicine, though the interpretation of any diagnostic results requires knowledge of test characteristics and the patient's history...
December 13, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27979739/genetic-variants-in-sirt3-transcriptional-regulatory-region-affect-promoter-activity-and-fat-deposition-in-three-cattle-breeds
#6
Linsheng Gui, Jieyun Hong, Sayed Haidar Abbas Raza, Linsen Zan
Sirtuin 3 (SIRT3) is a mitochondrial nicotinamide adenine dinucleotide (NAD)-dependent deacetylase. It has crucial roles in regulating the respiratory chain, in adenosine triphosphate (ATP) production, and in both the citric acid and urea cycles. The aim of this study was to investigate whether SIRT3 could be used as a candidate gene in the breeding of cattle. Expression analysis by quantitative real-time polymerase chain reactions (qPCR) indicated that expression levels of SIRT3 were highest in the kidney, rumen, liver, omasum and muscle...
December 12, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27919838/a-cgh-array-procedure-to-detect-pax6-gene-structural-defects
#7
Alessandra Franzoni, Patrizia Dello Russo, Federica Baldan, Angela Valentina D'Elia, Cinzia Puppin, Silvana Penco, Giuseppe Damante
Aniridia is a rare congenital disease characterized by eye development defects, in which the more evident clinical manifestation is iris absence or malformation. In most of the patients, aniridia is associated to PAX6 gene point mutations or deletions. When these deletions are large and involve other genes, a more complex disease, named WAGR syndrome, arises. In order to develop a new tool to analyze aniridia and WAGR subjects, a CGH array (CGHa) of the PAX6 genomic region was set up. We generated a custom microarray kit using an oligonucleotide-based platform that allows high resolution molecular profiling of genomic aberrations in 20 Mb of the 11p13 chromosomal region, centered on the PAX6 gene...
December 2, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27894847/pcr-screening-of-tick-borne-agents-in-sensitive-conservation-areas-southeast-portugal
#8
Maria Margarida Santos-Silva, Pedro Melo, Nuno Santos, Sandra Antunes, Luís Raposo Duarte, Joana Ferrolho, Natacha Milhano, Patrícia Tavares Santos, Ana Domingos, Ana Sofia Santos
The Southeast region of Portugal, particularly the Guadiana valley, is currently the reintroduction territory of Lynx pardinus (Iberian lynx), one of the most endangered felids in the world that is only found in the Iberian Peninsula. Over the last century, populations have declined, placing L. pardinus at extremely high risk of extinction in the wild and relying on reintroduction projects. Among the aspects taken into account in the establishment of new populations is the sanitary status of the selected habitats, especially concerning infectious diseases, including tick-borne pathogens (TBPs)...
November 25, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27890610/a-novel-real-time-pcr-assay-for-highly-specific-detection-and-quantification-of-vaginal-lactobacilli
#9
Vladimir V Demkin, Stanislav I Koshechkin, Alexei Slesarev
PCR detection and quantification of vaginal lactobacilli remains problematic because of the high level of genetic heterogeneity and taxonomic complexity within the genus Lactobacillus. The aim of the present study was to identify conserved sequences among the genomes of major species of vaginal lactobacilli that could be used for the development of a PCR-based method for quantitative determination of vaginal microbiota-specific lactobacilli. Comparative analysis of the genomes of several species of vaginal lactobacilli allowed us to identify conserved regions in the rplK gene, which encodes ribosomal protein L11, and to design group-specific PCR primers and a probe for selected species from the L...
November 25, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27871797/direct-pcr-a-rapid-method-for-multiplexed-detection-of-different-serotypes-of-salmonella-in-enriched-pork-meat-samples
#10
Wai Hoe Chin, Yi Sun, Jonas Høgberg, Than Linh Quyen, Pia Engelsmann, Anders Wolff, Dang Duong Bang
Salmonellosis, an infectious disease caused by Salmonella spp., is one of the most common foodborne diseases. Isolation and identification of Salmonella by conventional bacterial culture method is time consuming. In response to the demand for rapid on line or at site detection of pathogens, in this study, we developed a multiplex Direct PCR method for rapid detection of different Salmonella serotypes directly from pork meat samples without any DNA purification steps. An inhibitor-resistant Phusion Pfu DNA polymerase was used to overcome PCR inhibition...
November 18, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27856333/novel-homozygous-missense-mutation-in-aldh7a1-causes-neonatal-pyridoxine-dependent-epilepsy
#11
Emanuele G Coci, Luca Codutti, Christian Fink, Sophie Bartsch, Gunnar Grüning, Thomas Lücke, Ingo Kurth, Joachim Riedel
Pyridoxine dependent epilepsy (PDE) (OMIM#266100) is a neonatal form of epilepsy, caused by dysfunction of the enzyme α-aminoadipic semialdehyde dehydrogenase (ALDH7A1 or Antiquitin). This enzyme converts α-aminoadipic semialdehyde (α-AASA) into α-aminoadipate (AAA), a critical step in the lysine metabolism of the brain. ALDH7A1 dysfunction causes an accumulation of α-AASA and δ(1)-piperideine-6-carboxylic acid (P6C), which are in equilibrium with each other. P6C binds and inactivates pyridoxal 5'-phosphate (PLP), the active form of pyridoxine...
November 14, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27888871/dissecting-the-genetic-background-of-multifactorial-diseases-and-traits-a-major-challenge-for-genetic-research
#12
EDITORIAL
Sabine Hoffjan
No abstract text is available yet for this article.
December 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27818248/genetics-of-parkinson-s-disease
#13
REVIEW
Christina M Lill
Almost two decades after the identification of SNCA as the first causative gene in Parkinson's disease (PD) and the subsequent understanding that genetic factors play a substantial role in PD development, our knowledge of the genetic architecture underlying this disease has vastly improved. Approximately 5-10% of patients suffer from a monogenic form of PD where autosomal dominant mutations in SNCA, LRRK2, and VPS35 and autosomal recessive mutations in PINK1, DJ-1, and Parkin cause the disease with high penetrance...
December 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27751841/if-genetic-variation-could-talk-what-genomic-data-may-teach-us-about-the-importance-of-gene-expression-regulation-in-the-genetics-of-autism
#14
REVIEW
Erika Yeh, Lauren A Weiss
Autism spectrum disorder (ASD) has been long known to have substantial genetic etiology. Much research has attempted to identify specific genes contributing to ASD risk with the goal of tying gene function to a molecular pathological explanation for ASD. A unifying molecular pathology would potentially increase understanding of what is going wrong during development, and could lead to diagnostic biomarkers or targeted preventative or therapeutic directions. We review past and current genetic mapping approaches and discuss major results, leading to the hypothesis that global dysregulation of gene or protein expression may be implicated in ASD rather than disturbance of brain-specific functions...
December 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27725295/trying-to-understand-the-genetics-of-atopic-dermatitis
#15
REVIEW
Susanne Stemmler, Sabine Hoffjan
Atopic dermatitis (AD) is a common and complex skin disease associated with both genetic and environmental factors. Loss-of-function mutations in the filaggrin gene, encoding a structural protein with an important role in epidermal barrier function, constitutes a well recognised susceptibility locus for AD. Further, genome-wide association studies (GWAS), including large meta-analyses, have discovered 38 additional susceptibility loci with genome-wide significance. However, the reported variations only explain a fraction of the overall heritability of AD...
December 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27618776/genomics-of-alzheimer-s-disease-value-of-high-throughput-genomic-technologies-to-dissect-its-etiology
#16
REVIEW
Giuseppe Tosto, Christiane Reitz
Late-onset Alzheimer's disease (AD), the most common neurodegenerative disorder in western countries, is clinically defined by progressive worsening in cognitive functions along with function and behavioral impairment. This ultimately results in complete incapacity and death. AD is a clinically and pathologically heterogeneous disease, and this is reflected by the numerous genetic findings that point to several diverse molecular mechanisms and pathways. Linkage, genome-wide association and next-generation sequencing studies have led to the identification of more than 20 novel susceptibility loci for AD...
December 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27559009/genetics-and-pathophysiology-of-granulomatosis-with-polyangiitis-gpa-and-its-main-autoantigen-proteinase-3
#17
REVIEW
Manfred Relle, Bernd Föhr, Federica Fasola, Andreas Schwarting
Granulomatosis with polyangiitis (GPA) is a severe autoimmune disease and one of the small vessel anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitides. Although its etiology and pathophysiology are still widely unknown, it is accepted that infections, environmental factors, epigenetic modifications, and a genetic predisposition provide the basis for this systemic disorder. GPA typically evolves into two phases: an initial phase characterized by ear, nose and throat (ENT) manifestations, such as chronic sinusitis and otitis, ulceration of the oral cavity and pharynx, as well as pulmonary nodules and a severe generalized phase, defined by the occurrence of rapidly progressive glomerulonephritis, pulmonary hemorrhage, and arthritis...
December 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27546889/a-genetic-basis-for-multiple-sclerosis-severity-red-herring-or-real
#18
REVIEW
Vilija G Jokubaitis, Helmut Butzkueven
Multiple Sclerosis (MS) is an autoimmune degenerative disease of the central nervous system, characterized by multifocal demyelination and neurodegeneration. The genetic architecture of MS is complex, where genetic risk has been attributed to over 100 polymorphic loci each with small odds ratios. MS is a highly heterogeneous disease with numerous clinical and paraclinical endophenotypes. To-date, no genetic variant has been associated with clinical outcome, however, evidence exists that MS outcomes, like risk, are to an extent also controlled by genetic variation...
December 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27417534/the-search-for-modifier-genes-in-huntington-disease-multifactorial-aspects-of-a-monogenic-disorder
#19
REVIEW
Larissa Arning
It is becoming increasingly evident that the underlying mutation of a single locus is often insufficient for the prediction of the comprehensive phenotype in human Mendelian disorders, implicating that there is no clear distinction between monogenic and complex traits. By definition, monogenic traits show a classic pattern of inheritance and are strongly influenced by variation within a single gene. However, many Mendelian traits that result in genetic disorders can have phenotypes that differ in subtle or profound ways such as severity, onset age and other associated phenotypic characteristics...
December 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27777104/west-nile-virus-circulation-in-vojvodina-serbia-mosquito-bird-horse-and-human-surveillance
#20
Dušan Petrić, Tamaš Petrović, Ivana Hrnjaković Cvjetković, Marija Zgomba, Vesna Milošević, Gospava Lazić, Aleksandra Ignjatović Ćupina, Diana Lupulović, Sava Lazić, Dragan Dondur, Slavica Vaselek, Aleksandar Živulj, Bratislav Kisin, Tibor Molnar, Djordje Janku, Dubravka Pudar, Jelena Radovanov, Mihaela Kavran, Gordana Kovačević, Budimir Plavšić, Aleksandra Jovanović Galović, Milan Vidić, Svetlana Ilić, Mina Petrić
Efforts to detect West Nile virus (WNV) in the Vojvodina province, northern Serbia, commenced with human and mosquito surveillance in 2005, followed by horse (2009) and wild bird (2012) surveillance. The knowledge obtained regarding WNV circulation, combined with the need for timely detection of virus activity and risk assessment resulted in the implementation of a national surveillance programme integrating mosquito, horse and bird surveillance in 2014. From 2013, the system showed highly satisfactory results in terms of area specificity (the capacity to indicate the spatial distribution of the risk for human cases of West Nile neuroinvasive disease - WNND) and sensitivity to detect virus circulation even at the enzootic level...
October 21, 2016: Molecular and Cellular Probes
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