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Molecular and Cellular Probes

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https://www.readbyqxmd.com/read/28192165/copy-number-variation-of-housekeeping-gene-rpl13a-in-rat-strains-selected-for-nervous-system-excitability
#1
Ruslan Kalendar, Alexander Belyayev, Tatiana Zachepilo, Alexander Vaido, Dmitry Maidanyuk, Alan H Schulman, Natalia Dyuzhikova
We evaluated copy number variation (CNV) for four genes in rat strains differing in nervous system excitability. rpl13a copy number is significantly reduced in hippocampus and bone marrow in rats with a high excitability threshold and stress. The observed phenomenon may be associated a role for rpl13a in lipid metabolism.
February 10, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28189797/rapid-detection-of-enterovirus-and-coxsackievirus-a10-by-a-taqman-based-duplex-one-step-real-time-rt-pcr-assay
#2
Jingfang Chen, Rusheng Zhang, Xinhua Ou, Dong Yao, Zheng Huang, Linzhi Li, Biancheng Sun
A TaqMan based duplex one-step real time RT-PCR (rRT-PCR) assay was developed for the rapid detection of Coxsackievirus A10 (CV-A10) and other enterovirus (EVs) in clinical samples. The assay was fully evaluated and found to be specific and sensitive. When applied in 115 clinical samples, a 100% diagnostic sensitivity in CV-A10 detection and 97.4% diagnostic sensitivity in other EVs were found.
February 8, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28188840/comparison-and-evaluation-of-conventional-rt-pcr-sybr-green-i-and-taqman-real-time-rt-pcr-assays-for-the-detection-of-porcine-epidemic-diarrhea-virus
#3
Xinrong Zhou, Tiansheng Zhang, Deping Song, Tao Huang, Qi Peng, Yanjun Chen, Anqi Li, Fanfan Zhang, Qiong Wu, Yu Ye, Yuxin Tang
Porcine epidemic diarrhea (PED) caused by porcine epidemic diarrhea virus (PEDV) is a highly contagious intestinal disease, resulting in substantial economic losses to the swine industry worldwide. In this study, three assays, namely a conventional reverse transcription-polymerase chain reaction (RT-PCR), a SYBR Green I real-time RT-PCR and a TaqMan real-time RT-PCR targeting the highly conserved M gene of PEDV, were developed and evaluated. Then, the analytical specificity, sensitivity and reproducibility of these assays were determined and compared...
February 7, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28179103/rapid-diagnosis-of-vibrio-owensii-responsible-for-shrimp-acute-hepatopancreatic-necrosis-disease-with-isothermal-recombinase-polymerase-amplification-assay
#4
Liyuan Liu, Luzhi Jiang, Yongxin Yu, Xiaoming Xia, Yingjie Pan, Shuling Yan, Yongjie Wang
A rapid and sensitive AHPND-RPA assay was developed for the specific detection of the AHPND-causing Vibrio owensii. The AHPND-RPA detected as few as 2 copies per reaction in 9.02 ± 0.66 min at 39 °C, and showed 100% positive predictive value and negative predictive value for AHPND diagnosis.
February 5, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28130122/bias-in-detection-of-mycobacterium-tuberculosis-polyclonal-infection-use-clinical-samples-or-cultures
#5
Ghazaleh Farmanfarmaei, Mansour Kargarpour Kamakoli, Hamid Reza Sadegh, Morteza Masoumi, Farid Abdolrahimi, Abolfazl Fateh, Nayereh Ebrahimzadeh, Gholamreza Javadi, Fatemeh Rahimi Jamnani, Farzam Vaziri, Seyed Davar Siadat
The application of MIRU-VNTR has unveiled that infection by Mycobacterium tuberculosis can be polyclonal. Our comparative study demonstrated that based on the studied samples (clinical specimen or culture) detection of polyclonal M. tuberculosis infection can be significantly different.
January 24, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28013043/a-field-evaluation-of-an-isothermal-dna-amplification-assay-for-the-detection-of-theileria-annulata-infection-in-cattle
#6
Jacinto Gomes, Marcos Santos, Ana Amaro, Isabel Pereira da Fonseca, Gabriela Santos-Gomes, João Inácio
A loop-mediated isothermal amplification (LAMP) assay was evaluated for the detection of Theileria annulata infection in cattle. The results were compared with a real-time PCR used for the quantification of T. annulata parasitaemia. One hundred bovine blood samples from 16 cattle farms were tested with LAMP and real-time PCR, with T. annulata DNA being detected in 66% and 67% of the samples, respectively. The results showed that the LAMP assay detects a parasitaemia as low as 0.00025%, indicating a high analytical sensitivity of LAMP for clinical diagnosis of bovine theileriosis...
December 22, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28013042/production-of-stable-gfp-expressing-neural-cells-from-p19-embryonal-carcinoma-stem-cells
#7
Hedayatollah Shirzad, Fariba Esmaeili, Shabnam Bakhshalizadeh, Marzieh Ebrahimie, Esmaeil Ebrahimie
Murine P19 embryonal carcinoma (EC) cells are convenient to differentiate into all germ layer derivatives. One of the advantages of P19 cells is that the exogenous DNA can be easily inserted into them. Here, at the first part of this study, we generated stable GFP-expressing P19 cells (P19-GFP(+)). FACS and western-blot analysis confirmed stable expression of GFP in the cells. We previously demonstrated the efficient induction of neuronal differentiation from mouse ES and EC cells by application of a neuroprotective drug, selegiline In the second part of this study selegiline was used to induce differentiation of P19-GFP(+) into stable GFP-expressing neuron-like cells...
December 21, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28011345/genotyping-of-friesian-horses-to-detect-a-hydrocephalus-associated-c-1423c-t-mutation-in-b3galnt2-using-pcr-rflp-and-pcr-pira-methods-frequency-in-stallion-horses-in-m%C3%A3-xico
#8
Miguel Angel Ayala-Valdovinos, Jorge Galindo-García, David Sánchez-Chiprés, Theodor Duifhuis-Rivera
Hydrocephalus in Friesian horses is an autosomal recessive hereditary disease that can result in an abortion, a stillbirth, or euthanization of a newborn foal. Here, the hydrocephalus-associated c.1423C > T mutation in B3GALNT2 gene was detected with PCR-RFLP and PCR-PIRA methods for horse genotyping. A preliminary genotyping survey was performed on 83 randomly selected Friesian stallion horses to determine the current allele frequency in Mexico. The frequency of the mutant T allele was 9.6%.
December 21, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27989424/designing-and-computational-validation-of-microarray-probe-sets-for-the-diagnosis-of-viruses-of-non-human-vertebrates
#9
Gaurava K Rai, Rubina Lawrence
Making specific oligonucleotide probes for diagnosis and differentiation of viruses has always been a challenging task due to unavailability of defined viral sequence databases, probe designing softwares and higher degree of sequence homology among the types and subtypes of viruses. We have provided here, a streamlined procedure to design microarray probes involving various computational tools which is capable of yielding highly specific as well as conserved oligonucleotides to facilitate unbiased diagnosis of viruses...
December 15, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27986555/serological-approaches-for-the-diagnosis-of-schistosomiasis-a-review
#10
Rebecca Hinz, Norbert G Schwarz, Andreas Hahn, Hagen Frickmann
Schistosomiasis is a common disease in endemic areas of Sub-Saharan Africa, South America and Asia. It is rare in Europe, mainly imported from endemic countries due to travelling or human migration. Available methods for the diagnosis of schistosomiasis comprise microscopic, molecular and serological approaches, with the latter detecting antigens or antibodies associated with Schistosoma spp. infection. The serological approach is a valuable screening tool in low-endemicity settings and for travel medicine, though the interpretation of any diagnostic results requires knowledge of test characteristics and a patient's history...
December 13, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27979739/genetic-variants-in-sirt3-transcriptional-regulatory-region-affect-promoter-activity-and-fat-deposition-in-three-cattle-breeds
#11
Linsheng Gui, Jieyun Hong, Sayed Haidar Abbas Raza, Linsen Zan
Sirtuin 3 (SIRT3) is a mitochondrial nicotinamide adenine dinucleotide (NAD)-dependent deacetylase. It has crucial roles in regulating the respiratory chain, in adenosine triphosphate (ATP) production, and in both the citric acid and urea cycles. The aim of this study was to investigate whether SIRT3 could be used as a candidate gene in the breeding of cattle. Expression analysis by quantitative real-time polymerase chain reactions (qPCR) indicated that expression levels of SIRT3 were highest in the kidney, rumen, liver, omasum and muscle...
December 12, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27919838/a-cgh-array-procedure-to-detect-pax6-gene-structural-defects
#12
Alessandra Franzoni, Patrizia Dello Russo, Federica Baldan, Angela Valentina D'Elia, Cinzia Puppin, Silvana Penco, Giuseppe Damante
Aniridia is a rare congenital disease characterized by eye development defects, in which the more evident clinical manifestation is iris absence or malformation. In most of the patients, aniridia is associated to PAX6 gene point mutations or deletions. When these deletions are large and involve other genes, a more complex disease, named WAGR syndrome, arises. In order to develop a new tool to analyze aniridia and WAGR subjects, a CGH array (CGHa) of the PAX6 genomic region was set up. We generated a custom microarray kit using an oligonucleotide-based platform that allows high resolution molecular profiling of genomic aberrations in 20 Mb of the 11p13 chromosomal region, centered on the PAX6 gene...
December 2, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27894847/pcr-screening-of-tick-borne-agents-in-sensitive-conservation-areas-southeast-portugal
#13
Maria Margarida Santos-Silva, Pedro Melo, Nuno Santos, Sandra Antunes, Luís Raposo Duarte, Joana Ferrolho, Natacha Milhano, Patrícia Tavares Santos, Ana Domingos, Ana Sofia Santos
The Southeast region of Portugal, particularly the Guadiana valley, is currently the reintroduction territory of Lynx pardinus (Iberian lynx), one of the most endangered felids in the world that is only found in the Iberian Peninsula. Over the last century, populations have declined, placing L. pardinus at extremely high risk of extinction in the wild and relying on reintroduction projects. Among the aspects taken into account in the establishment of new populations is the sanitary status of the selected habitats, especially concerning infectious diseases, including tick-borne pathogens (TBPs)...
November 25, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27890610/a-novel-real-time-pcr-assay-for-highly-specific-detection-and-quantification-of-vaginal-lactobacilli
#14
Vladimir V Demkin, Stanislav I Koshechkin, Alexei Slesarev
PCR detection and quantification of vaginal lactobacilli remains problematic because of the high level of genetic heterogeneity and taxonomic complexity within the genus Lactobacillus. The aim of the present study was to identify conserved sequences among the genomes of major species of vaginal lactobacilli that could be used for the development of a PCR-based method for quantitative determination of vaginal microbiota-specific lactobacilli. Comparative analysis of the genomes of several species of vaginal lactobacilli allowed us to identify conserved regions in the rplK gene, which encodes ribosomal protein L11, and to design group-specific PCR primers and a probe for selected species from the L...
November 25, 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28187875/editorial-vectors-and-vbds
#15
EDITORIAL
Hans-Peter Fuehrer, Sara Savić
No abstract text is available yet for this article.
February 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27888871/dissecting-the-genetic-background-of-multifactorial-diseases-and-traits-a-major-challenge-for-genetic-research
#16
EDITORIAL
Sabine Hoffjan
No abstract text is available yet for this article.
December 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27818248/genetics-of-parkinson-s-disease
#17
REVIEW
Christina M Lill
Almost two decades after the identification of SNCA as the first causative gene in Parkinson's disease (PD) and the subsequent understanding that genetic factors play a substantial role in PD development, our knowledge of the genetic architecture underlying this disease has vastly improved. Approximately 5-10% of patients suffer from a monogenic form of PD where autosomal dominant mutations in SNCA, LRRK2, and VPS35 and autosomal recessive mutations in PINK1, DJ-1, and Parkin cause the disease with high penetrance...
December 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27751841/if-genetic-variation-could-talk-what-genomic-data-may-teach-us-about-the-importance-of-gene-expression-regulation-in-the-genetics-of-autism
#18
REVIEW
Erika Yeh, Lauren A Weiss
Autism spectrum disorder (ASD) has been long known to have substantial genetic etiology. Much research has attempted to identify specific genes contributing to ASD risk with the goal of tying gene function to a molecular pathological explanation for ASD. A unifying molecular pathology would potentially increase understanding of what is going wrong during development, and could lead to diagnostic biomarkers or targeted preventative or therapeutic directions. We review past and current genetic mapping approaches and discuss major results, leading to the hypothesis that global dysregulation of gene or protein expression may be implicated in ASD rather than disturbance of brain-specific functions...
December 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27725295/trying-to-understand-the-genetics-of-atopic-dermatitis
#19
REVIEW
Susanne Stemmler, Sabine Hoffjan
Atopic dermatitis (AD) is a common and complex skin disease associated with both genetic and environmental factors. Loss-of-function mutations in the filaggrin gene, encoding a structural protein with an important role in epidermal barrier function, constitutes a well recognised susceptibility locus for AD. Further, genome-wide association studies (GWAS), including large meta-analyses, have discovered 38 additional susceptibility loci with genome-wide significance. However, the reported variations only explain a fraction of the overall heritability of AD...
December 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/27618776/genomics-of-alzheimer-s-disease-value-of-high-throughput-genomic-technologies-to-dissect-its-etiology
#20
REVIEW
Giuseppe Tosto, Christiane Reitz
Late-onset Alzheimer's disease (AD), the most common neurodegenerative disorder in western countries, is clinically defined by progressive worsening in cognitive functions along with function and behavioral impairment. This ultimately results in complete incapacity and death. AD is a clinically and pathologically heterogeneous disease, and this is reflected by the numerous genetic findings that point to several diverse molecular mechanisms and pathways. Linkage, genome-wide association and next-generation sequencing studies have led to the identification of more than 20 novel susceptibility loci for AD...
December 2016: Molecular and Cellular Probes
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