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Genome Génome / Conseil National de Recherches Canada

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https://www.readbyqxmd.com/read/28084809/mitochondrial-genome-of-the-sweet-potato-hornworm-agrius-convolvuli-lepidoptera-sphingidae-and-comparison-with-other-lepidoptera-species
#1
Li-Shang Dai, Sheng Li, Hui-Min Yu, Guo-Qing Wei, Lei Wang, Cen Qian, Cong-Fen Zhang, Jun Li, Yu Sun, Yue Zhao, Bao-Jian Zhu, Chao-Liang Liu
In the present study, we sequenced the complete mitochondrial genome (mitogenome) of Agrius convolvuli (Lepidoptera: Sphingidae) and compared it with previously sequenced mitogenomes of lepidopteran species. The mitogenome was a circular molecule, 15 349 base pairs (bp) long, containing 37 genes. The order and orientation of genes in the A. convolvuli mitogenome were similar to those in sequenced mitogenomes of other lepidopterans. All 13 protein-coding genes (PCGs) were initiated by ATN codons, except for the cytochrome c oxidase subunit 1 (cox1) gene, which seemed to be initiated by the codon CGA, as observed in other lepidopterans...
October 21, 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28044453/contrasting-morphological-and-dna-barcode-suggested-species-boundaries-among-shallow-water-amphipod-fauna-from-the-southern-european-atlantic-coast
#2
Jorge Lobo, Maria S Ferreira, Ilisa C Antunes, Marcos A L Teixeira, Luisa M S Borges, Ronaldo Sousa, Pedro A Gomes, Maria Helena Costa, Marina R Cunha, Filipe O Costa
In this study we compared DNA barcode-suggested species boundaries with morphology-based species identifications in the amphipod fauna of the southern European Atlantic coast. DNA sequences of the cytochrome c oxidase subunit I barcode region (COI-5P) were generated for 43 morphospecies (178 specimens) collected along the Portuguese coast which, together with publicly available COI-5P sequences, produced a final dataset comprising 68 morphospecies and 295 sequences. Seventy-five BINs (Barcode Index Numbers) were assigned to these morphospecies, of which 48 were concordant (i...
October 21, 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27996306/mapping-qtls-associated-with-agronomic-and-physiological-traits-under-terminal-drought-and-heat-stress-conditions-in-wheat-triticum-aestivum-l
#3
Sirous Tahmasebi, Bahram Heidari, Hassan Pakniyat, C Lynne McIntyre
Wheat crops frequently experience a combination of abiotic stresses in the field, but most quantitative trait loci (QTL) studies have focused on the identification of QTLs for traits under single stress field conditions. A recombinant inbred line (RIL) population derived from SeriM82 × Babax was used to map QTLs under well-irrigated, heat, drought, and a combination of heat and drought stress conditions in two years. A total of 477 DNA markers were used to construct linkage groups that covered 1619.6 cM of the genome, with an average distance of 3...
January 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27996299/dna-recovery-from-microhymenoptera-using-six-non-destructive-methodologies-with-considerations-for-subsequent-preparation-of-museum-slides
#4
Adriana J Guzmán-Larralde, Alba P Suaste-Dzul, Adrien Gallou, Kenzy I Peña-Carrillo
Because of the tiny size of microhymenoptera, successful morphological identification typically requires specific mounting protocols that require time, skills, and experience. Molecular taxonomic identification is an alternative, but many DNA extraction protocols call for maceration of the whole specimen, which is not compatible with preserving museum vouchers. Thus, non-destructive DNA isolation methods are attractive alternatives for obtaining DNA without damaging sample individuals. However, their performance needs to be assessed in microhymenopterans...
January 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27936933/review-of-genetic-diversification-of-bats-in-the-caribbean-and-biogeographic-relationships-to-neotropical-species-based-on-dna-barcodes
#5
Burton K Lim
DNA barcoding is helping in discovering high levels of cryptic species and an underestimation of biodiversity in many groups of organisms. Although mammals are arguably the most studied and one of the least speciose taxonomic classes, the rate of species discovery is increasing and biased for small mammals on islands. An earlier study found bats in the Caribbean as a taxonomic and geographic deficiency in the International Barcode of Life initiative to establish a genetic reference database to enable specimen identification to species...
January 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27936922/dissimilar-evolutionary-histories-of-two-resistance-gene-families-in-the-genus-solanum
#6
Diana María Segura, Ricardo Williams Masuelli, M Virginia Sanchez-Puerta
Genomic analyses have shown that most genes in eukaryotic lineages belong to families. Gene families vary in terms of number of members, nucleotide similarity, gene integrity, expression, and function. Often, the members of gene families are arranged in clusters, which contribute to maintaining similarity among gene copies and also to generate duplicates through replication errors. Gene families offer us an opportunity to examine the forces involved in the evolution of the genomes and to study recombination events and genomic rearrangements...
January 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27936908/the-fbxo7-homologue-nutcracker-and-binding-partner-pi31-in-drosophila-melanogaster-models-of-parkinson-s-disease
#7
Eric M Merzetti, Lindsay A Dolomount, Brian E Staveley
Parkinsonian-pyramidal syndrome (PPS) is an early onset form of Parkinson's disease (PD) that shows degeneration of the extrapyramidal region of the brain to result in a severe form of PD. The toxic protein build-up has been implicated in the onset of PPS. Protein removal is mediated by an intracellular proteasome complex: an E3 ubiquitin ligase, the targeting component, is essential for function. FBXO7 encodes the F-box component of the SCF E3 ubiquitin ligase linked to familial forms of PPS. The Drosophila melanogaster homologue nutcracker (ntc) and a binding partner, PI31, have been shown to be active in proteasome function...
January 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27922272/diversity-of-indoor-fungi-as-revealed-by-dna-metabarcoding
#8
Helena Korpelainen, Maria Pietiläinen
In the present study, we conducted DNA metabarcoding (the nuclear ITS2 region) for indoor fungal samples originating from two nursery schools with a suspected mould problem (sampling before and after renovation), from two university buildings, and from an old farmhouse. Good-quality sequences were obtained, and the results showed that DNA metabarcoding provides high resolution in fungal identification. The pooled proportions of sequences representing filamentous ascomycetes, filamentous basidiomycetes, yeasts, and other fungi equalled 62...
January 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27922269/genomic-study-of-the-type-ivc-secretion-system-in-clostridium-difficile-understanding-c-difficile-evolution-via-horizontal-gene-transfer
#9
Wen Zhang, Ying Cheng, Pengcheng Du, Yuanyuan Zhang, Hongbing Jia, Xianping Li, Jing Wang, Na Han, Yujun Qiang, Chen Chen, Jinxing Lu
Clostridium difficile, the etiological agent of Clostridium difficile infection (CDI), is a gram-positive, spore-forming bacillus that is responsible for ∼20% of antibiotic-related cases of diarrhea and nearly all cases of pseudomembranous colitis. Previous data have shown that a substantial proportion (11%) of the C. difficile genome consists of mobile genetic elements, including seven conjugative transposons. However, the mechanism underlying the formation of a mosaic genome in C. difficile is unknown. The type-IV secretion system (T4SS) is the only secretion system known to transfer DNA segments among bacteria...
January 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27918193/dna-barcodes-identify-medically-important-tick-species-in-canada
#10
Danielle A Ondrejicka, Kevin C Morey, Robert H Hanner
Medically important ticks (Acari: Ixodidae) are often difficult to identify morphologically. A standardized, molecular approach using a 658 base pair DNA barcode sequence (from the 5' region of the mitochondrial cytochrome c oxidase subunit I gene) was evaluated for its effectiveness in discriminating ticks in North America, with an emphasis on Canadian ticks. DNA barcodes were generated for 96 of 154 specimens representing 26 ixodid species. A genetic cluster analysis was performed on the barcode sequences, which separated specimens into haplogroups closely corresponding with morphologically identified species...
January 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27848260/the-htra2-drosophila-model-of-parkinson-s-disease-is-suppressed-by-the-pro-survival-bcl-2-buffy
#11
P Githure M'Angale, Brian E Staveley
Mutations in High temperature requirement A2 (HtrA2), also designated PARK13, which lead to the loss of its protease activity, have been associated with Parkinson's disease (PD). HtrA2 is a mitochondrial protease that translocates to the cytosol upon the initiation of apoptosis where it participates in the abrogation of inhibitors of apoptosis (IAP) inhibition of caspases. Here, we demonstrate that the loss of the HtrA2 function in the dopaminergic neurons of Drosophila melanogaster results in PD-like phenotypes, and we attempt to restore the age-dependent loss in locomotor ability by co-expressing the sole pro-survival Bcl-2 homologue Buffy...
January 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27897070/note-of-appreciation-note-de-reconnaissance
#12
(no author information available yet)
No abstract text is available yet for this article.
December 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27897069/announcement-communiqu%C3%A3
#13
(no author information available yet)
No abstract text is available yet for this article.
December 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27845571/the-importance-of-taxonomic-resolution-for-additive-beta-diversity-as-revealed-through-dna-barcoding
#14
Trevor T Bringloe, Karl Cottenie, Gillian K Martin, Sarah J Adamowicz
Additive diversity partitioning (α, β, and γ) is commonly used to study the distribution of species-level diversity across spatial scales. Here, we first investigate whether published studies of additive diversity partitioning show signs of difficulty attaining species-level resolution due to inherent limitations with morphological identifications. Second, we present a DNA barcoding approach to delineate specimens of stream caddisfly larvae (order Trichoptera) and consider the importance of taxonomic resolution on classical (additive) measures of beta (β) diversity...
December 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27831816/genome-wide-analysis-of-the-dna-binding-with-one-zinc-finger-dof-transcription-factor-family-in-bananas
#15
Chen Dong, Huigang Hu, Jianghui Xie
DNA-binding with one finger (Dof) domain proteins are a multigene family of plant-specific transcription factors involved in numerous aspects of plant growth and development. In this study, we report a genome-wide search for Musa acuminata Dof (MaDof) genes and their expression profiles at different developmental stages and in response to various abiotic stresses. In addition, a complete overview of the Dof gene family in bananas is presented, including the gene structures, chromosomal locations, cis-regulatory elements, conserved protein domains, and phylogenetic inferences...
December 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27824505/molecular-taxonomic-analysis-of-the-plant-associations-of-adult-pollen-beetles-nitidulidae-meligethinae-and-the-population-structure-of-brassicogethes-aeneus
#16
Pierre Ouvrard, Damien M Hicks, Molly Mouland, James A Nicholls, Katherine C R Baldock, Mark A Goddard, William E Kunin, Simon G Potts, Thomas Thieme, Eve Veromann, Graham N Stone
Pollen beetles (Nitidulidae: Meligethinae) are among the most abundant flower-visiting insects in Europe. While some species damage millions of hectares of crops annually, the biology of many species is little known. We assessed the utility of a 797 base pair fragment of the cytochrome oxidase 1 gene to resolve molecular operational taxonomic units (MOTUs) in 750 adult pollen beetles sampled from flowers of 63 plant species sampled across the UK and continental Europe. We used the same locus to analyse region-scale patterns in population structure and demography in an economically important pest, Brassicogethes aeneus...
December 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27819140/de-novo-balanced-complex-chromosome-rearrangements-involving-chromosomes-1b-and-3b-of-wheat-and-1r-of-rye
#17
Tianheng Ren, Zhi Li, Benju Yan, Feiquan Tan, Zongxiang Tang, Shulan Fu, Manyu Yang, Zhenglong Ren
Complex chromosome rearrangements (CCRs) are defined as structural abnormalities involving more than two chromosome breaks, coupled with exchanges of chromosomal segments. Information on CCRs in plants is limited. In the present study, a plant (26-4) harboring translocation chromosomes 1RS.1BL and 4RS.4DL was selected from a double monosomic (1R and 4R) addition line, which was derived from the hybrid between wheat cultivar MY11 and a Chinese local rye variety. The genome of the plant with double alien translocation chromosomes in the monosomic form showed more instability than that harboring a single translocation...
December 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27753524/multi-locus-dna-barcoding-identifies-matk-as-a-suitable-marker-for-species-identification-in-hibiscus-l
#18
Sundar Poovitha, Nithaniyal Stalin, Raju Balaji, Madasamy Parani
The genus Hibiscus L. includes several taxa of medicinal value and species used for the extraction of natural dyes. These applications require the use of authentic plant materials. DNA barcoding is a molecular method for species identification, which helps in reliable authentication by using one or more DNA barcode marker. In this study, we have collected 44 accessions, representing 16 species of Hibiscus, distributed in the southern peninsular India, to evaluate the discriminatory power of the two core barcodes rbcLa and matK together with the suggested additional regions trnH-psbA and ITS2...
December 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27753507/identifying-pelagic-fish-eggs-in-the-southeast-yucatan-peninsula-using-dna-barcodes
#19
E Leyva-Cruz, L Vásquez-Yeomans, L Carrillo, M Valdez-Moreno
In the waters surrounding Banco Chinchorro in the Mexican Caribbean are spawning and nursery areas for many types of fish. In this natural environment, as opposed to under controlled laboratory conditions, it is almost impossible to link an individual egg to the adult that laid it. This makes identifying the species of the eggs difficult. However, DNA barcodes have made this easier. In the present study, 300 eggs were processed for molecular analysis, from which 139 sequences were obtained. We identified 42 taxa (33 species with their binomial names), 35 genera, and 24 families...
December 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27753500/development-of-species-specific-primers-with-potential-for-amplifying-edna-from-imperilled-freshwater-unionid-mussels
#20
Anna Cho, Todd Morris, Chris Wilson, Joanna Freeland
Environmental DNA (eDNA) is emerging as a potentially powerful tool for inferring species' presence, and hence occupancy, from DNA that is shed into environmental samples such as water. Although eDNA screening has been used to detect DNA from a variety of taxonomic groups, it has not yet been used to identify DNA from species with numerous potentially sympatric confamilial species, a situation that may preclude the development of species-specific markers. There are 41 native freshwater mussel species (Unionidae) in Ontario, Canada...
December 2016: Genome Génome / Conseil National de Recherches Canada
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