journal
MENU ▼
Read by QxMD icon Read
search

Genome Génome / Conseil National de Recherches Canada

journal
https://www.readbyqxmd.com/read/28235186/differential-transferability-of-est-ssr-primers-developed-from-diploid-species-i-pseudoroegneria-spicata-i-i-thinopyrum-bessarabicum-i-and-i-th-elongatum-i
#1
Richard R-C Wang, Steven R Larson, Kevin B Jensen
Simple sequence repeat technology based on expressed sequence tag (EST-SSR) is a useful genomic tool for genome mapping, characterizing plant species relationships, elucidating genome evolution, and tracing genes on alien chromosome segments. EST-SSR primers developed from three perennial diploid Triticeae species, <i>Pseudoroegneria spicata</I> (Pursh) Á. Löve (having <b>St</b> genome), <i>Thinopyrum bessarabicum</I> (Savul. & Rayss) Á. Löve (<b>J<sup>b</sup></b> = <b>E<sup>b</sup></b> = <b>J</b>), and <i>Th...
February 24, 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28177825/enhancement-of-single-guide-rna-transcription-for-efficient-crispr-cas-based-genomic-engineering
#2
Kumiko Ui-Tei, Shohei Maruyama, Yuko Nakano
Genomic engineering using clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) protein is a promising approach for targeting the genomic DNA of virtually any organism in a sequence-specific manner. Recent remarkable advances in CRISPR/Cas technology have made it a feasible system for use in therapeutic applications and biotechnology. In the CRISPR/Cas system, a guide RNA (gRNA), interacting with the Cas protein, recognizes a genomic region with sequence complementarity, and the double-stranded DNA at the target site is cleaved by the Cas protein...
January 26, 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28177827/genomic-constitution-and-intergenomic-translocations-in-elymus-dahuricus-complex-revealed-by-multicolor-gish
#3
Cairong Yang, Zhang Haiqin, Weihuan Chen, Houyang Kang, Yi Wang, Sha Lina, Xing Fan, Jian Zeng, Yong-Hong Zhou
Multicolor genomic in situ hybridization was used to investigate genomic constitution and intergenomic translocations in Elymus dahuricus complex. The genomic constitution of species in E. dahuricus complex was confirmed as StYH. H/Y and H/St intergenomic translocations were identified in the present study with 7H and 1Y chromosomes involved in reciprocal translocations for all the accessions investigated in the complex. We propose that the translocations are species-specific in the E. dahuricus complex, are associated with allopolyploidy and may serve as important structural alterations for allopolyploid stability...
January 11, 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28177826/phylogenetic-analysis-of-two-single-copy-nuclear-genes-revealed-origin-and-complex-relationships-of-hordeum-polyploids-in-triticeae-poaceae
#4
Qianni Hu, Genlou Sun
Two single copy nuclear genes, the second largest subunit of RNA polymerase II (RPB2) and thioredoxin-like gene (HTL), were used to explore the phylogeny and origin of polyploid species in Hordeum. Our results were partly in accord with previous studies, but disclosed additional complexity. Both RPB2 and HTL trees confirmed the presence of Xa genome in H. capense and H. secalinum, and that H. depressum originated from H. californicum together with other American diploids, either H. intercedens or H. pusillum...
January 11, 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28177829/genomic-and-metabolic-characterisation-of-alkaloid-biosynthesis-by-asexual-epichlo%C3%A3-fungal-endophytes-of-tall-fescue-pasture-grasses
#5
Piyumi Ekanayake, Jatinder Kaur, Pei Tian, Simone Rochfort, Kathryn Guthridge, Tim Sawbridge, German Spangenberg, John Forster
Symbiotic associations between tall fescue grasses and asexual Epichloë fungal endophytes exhibit biosynthesis of alkaloid compounds causing both beneficial and detrimental effects. Candidate novel endophytes with favourable chemotypic profiles have been identified in germplasm collections by screening for genetic diversity, followed by metabolite profile analysis in endogenous genetic backgrounds. A sub-set of candidates was subjected to genome survey sequencing to detect the presence or absence and structural status of known genes for biosynthesis of the major alkaloid classes...
January 4, 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28177828/isolation-and-characterization-of-a-novel-pollen-specific-promoter-in-maize%C3%AF-i-zea-mays-l-i-%C3%AF
#6
He Wang, Mingxia Fan, Guohong Wang, Chunyu Zhang, Lei Shi, Zhengyi Wei, Wenjuan Ma, Jing Chang, Senxin Huang, Feng Lin
<i>ZmSTK2_USP</i>, located on the long arm of chromosome 4, belongs to the serine/threonine kinase gene in maize. The sequence analysis of 2,100 bp upstream from the start codon ATG has shown that it contains <i>cis</i>-element motifs and two types of anther/pollen-specific promoter elements (GTGA and AGAAA), suggesting that it is the pollen-specific promoter. To investigate the function of <i>ZmSTK2_USP</i> promoter, the GUS gene fusion system was employed. In pro<i>ZmSTK2_USP-GUS</i> genetically modified plants, GUS activity was detected in mature pollen grains and pollen tubes but not found in other floral and vegetable tissues...
January 4, 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28177830/analysis-of-codon-usage-patterns-in-morus-notabilis-based-on-genome-and-transcriptome-data
#7
Yan Wen, Ziliang Zou, Hongshun Li, Zhonghuai Xiang, Ningjia He
Codons play important roles in regulating gene expression levels and mRNA half-lives. However, codon usage and related studies in multicellular organisms still lag far behind those in unicellular organisms. In this study, we describe for the first time genome-wide patterns of codon bias in Morus notabilis (mulberry tree), and analyze genome-wide codon usage in 12 other Rosales species. The codon usage of M. notabilis was affected by nucleotide composition, mutation pressure, nature selection, and gene expression level...
January 3, 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28177831/rapid-identification-of-a-new-gene-influencing-low-amylose-content-in-rice-landraces-oryza-sativa-l-using-genome-wide-association-study-with-specific-locus-amplified-fragment-sequencing
#8
Xinghai Yang, Baoxuan Nong, Xiuzhong Xia, Zongqiong Zhang, Yu Zeng, Kaiqiang Liu, Guofu Deng, Danting Li
Starch is the major compoments of milled rice, and amylose content (AC) affects eating quality. In this study, genome-wide association study (GWAS) with specific-locus amplified fragment sequencing (SLAF-seq) data was performed for AC in a panel of 419 core collection for rice landraces. Using the compressed mixed linear method based on the Q+K model, we identified a new gene LAC6 (Chr6: 5.65 Mb ~ 6.04 Mb) associated with AC in the low amylose content panel. The candidate genes of LAC6 was detected through qRT-PCR in rice panicles...
December 23, 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28177833/the-importance-of-molecular-markers-and-primer-design-when-characterizing-biodiversity-from-environmental-dna-edna
#9
Joanna Freeland
Environmental DNA (eDNA) comprises DNA fragments that have been shed into the environment by organisms, and which can be extracted from environmental samples such as water or soil. Characterization of eDNA can allow researchers to infer the presence or absence of species from a particular site without the need to locate and identify individuals, and therefore may provide an extremely valuable tool for quantifying biodiversity. However, as is often the case with relatively new protocols, methodological challenges remain...
December 22, 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28177832/natural-variation-in-i-circadian-clock-associated-1-i-is-associated-with-flowering-time-in-i-brassica-rapa-i
#10
Hankuil Yi, Xiaonan Li, Seong Ho Lee, Ill-Sup Nou, Yong Pyo Lim, Yoonkang Hur
Flowering time is a very important agronomic trait and the development of molecular markers associated with this trait can facilitate crop breeding. <i>CIRCADIAN CLOCK ASSOCIATED 1</i> (<i>CCA1</i>), a core oscillator component of circadian rhythms that affect metabolic pathways in plants, has been implicated in flowering time control in <i>Brassica</i> species. <i>CCA1</i> gene sequences from three <i>Brassica rapa</i> inbred lines, showing either early flowering or late flowering phenotypes, were analyzed and a high level of ets were designed and tested using various inbred lines of <i>B...
December 22, 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28106473/overexpression-of-buffy-enhances-the-loss-of-parkin-and-suppresses-the-loss-of-pink1-phenotypes-in-drosophila
#11
P Githure M'Angale, Brian E Staveley
Mutations in parkin (PARK2) and Pink1 (PARK6) are responsible for autosomal recessive forms of early onset Parkinson's disease (PD). Attributed to the failure of neurons to clear dysfunctional mitochondria, loss of gene expression leads to loss of nigrostriatal neurons. The Pink1/parkin pathway plays a role in the quality control mechanism aimed at eliminating defective mitochondria, and the failure of this mechanism results in a reduced lifespan and impaired locomotor ability, among other phenotypes. Inhibition of parkin or Pink1 through the induction of stable RNAi transgene in the Ddc-Gal4-expressing neurons results in such phenotypes to model PD...
December 22, 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28121461/the-dynamic-evolutionary-history-of-genome-size-in-north-american-woodland-salamanders
#12
Catherine E Newman, T Ryan Gregory, Christopher C Austin
The genus Plethodon is the most species-rich salamander genus in North America, and nearly half of its species face an uncertain future. It is also one of the most diverse families in terms of genome sizes, which range from 1C = 18.2 to 69.3 pg, or 5-20 times larger than the human genome. Large genome size in salamanders results in part from accumulation of transposable elements and is associated with various developmental and physiological traits. However, genome sizes have been reported for only 25% of the species of Plethodon (14 of 55)...
December 11, 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28177836/microsatellite-markers-reveal-low-frequency-of-natural-hybridization-between-the-white-footed-mouse-peromyscus-leucopus-and-deer-mouse-peromyscus-maniculatus-in-southern-quebec-canada
#13
Sarah Sin Tong Leo, Virginie Millien
In some parts of southern Quebec, two closely related rodent species - the white-footed mouse (Peromyscus leucopus) and the deer mouse (P. maniculatus) have recently come in contact due to climate-driven changes in the distribution of the former. Both species share similar morphology, ecology, and life history traits, which suggests that natural hybridization may be possible. Hybridization among these two species can have important implications on the ecological roles these rodents play in disease transmission, yet few researchers have attempted to examine this phenomenon and results from previous hybridization experiments have remained inconclusive and conflicting...
December 10, 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28177835/analysis-of-the-histone-cluster-in-senegalese-sole-solea-senegalensis-evidence-for-a-divergent-evolution-of-two-canonical-histone-clusters
#14
Manuel Alejandro Merlo, Roger Iziga, Silvia Portela, Ismael Cross, Nadezda Kosyakova, Thomas Liehr, Manuel Manchado, Laureana Rebordinos
The Senegalese sole (Solea senegalensis) is commercially very important, and is a priority species for aquaculture product diversification. The main histone cluster was found in two BAC clones. However, two replacement histones (H1.0 and H3.3) were found in another BAC clone. Different types of canonical H2A and H2B have been found in one species for the first time. Phylogenetic analysis demonstrated that the different H1, H2A and H2B types were all more similar to each other than to canonical histones from other species...
December 10, 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28177834/cytogenetic-study-and-stripe-rust-response-of-the-derivatives-from-a-wheat-thinopyrum-intermedium-psathyrostachys-huashanica-trigeneric-hybrid
#15
Kang Houyang, Lin Tang, Daiyan Li, Chengdou Diao, Wei Zhu, Yao Tang, Yi Wang, Xing Fan, Lili Xu, Jian Zeng, Sha Lina, Xiaofang Yu, Zhang Haiqin, Yong-Hong Zhou
In this work, the meiotic behavior, chromosome constitution, and stripe rust resistance of the F5 derivatives from wheat-Th. intermedium-P. huashanica trigeneric hybrid were studied. Cytological analysis indicated that the chromosome number of F5 progenies ranged from 42 to 50. The mean meiotic configuration of F5 lines was 1.28 univalents, 21.74 bivalents, 0.04 trivalents, and 0.02 tetravalents per pollen mother cell (PMC). Among the lines with 2n = 42, the average pairing configuration was 0.05 I + 19.91 II (ring) + 1...
December 10, 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28094568/barcode-based-species-delimitation-in-the-marine-realm-a-test-using-hexanauplia-multicrustacea-thecostraca-and-copepoda
#16
Robert G Young, Cathryn L Abbott, Thomas W Therriault, Sarah J Adamowicz
DNA barcoding has been used successfully for identifying specimens belonging to marine planktonic groups. However, the ability to delineate species within taxonomically diverse and widely distributed marine groups, such as the Copepoda and Thecostraca, remains largely untested. We investigate whether a cytochrome c oxidase subunit I (COI-5P) global pairwise sequence divergence threshold exists between intraspecific and interspecific divergences in the copepods plus the thecostracans (barnacles and allies). Using publicly accessible sequence data, we applied a graphical method to determine an optimal threshold value...
February 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28092167/a-semi-nested-real-time-pcr-method-to-detect-low-chimerism-percentage-in-small-quantity-of-hematopoietic-stem-cell-transplant-dna-samples
#17
Michelangelo Aloisio, Barbara Bortot, Ilaria Gandin, Giovanni Maria Severini, Emmanouil Athanasakis
Chimerism status evaluation of post-allogeneic hematopoietic stem cell transplantation samples is essential to predict post-transplant relapse. The most commonly used technique capable of detecting small increments of chimerism is quantitative real-time PCR. Although this method is already used in several laboratories, previously described protocols often lack sensitivity and the amount of the DNA required for each chimerism analysis is too high. In the present study, we compared a novel semi-nested allele-specific real-time PCR (sNAS-qPCR) protocol with our in-house standard allele-specific real-time PCR (gAS-qPCR) protocol...
February 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28084809/mitochondrial-genome-of-the-sweet-potato-hornworm-agrius-convolvuli-lepidoptera-sphingidae-and-comparison-with-other-lepidoptera-species
#18
Li-Shang Dai, Sheng Li, Hui-Min Yu, Guo-Qing Wei, Lei Wang, Cen Qian, Cong-Fen Zhang, Jun Li, Yu Sun, Yue Zhao, Bao-Jian Zhu, Chao-Liang Liu
In the present study, we sequenced the complete mitochondrial genome (mitogenome) of Agrius convolvuli (Lepidoptera: Sphingidae) and compared it with previously sequenced mitogenomes of lepidopteran species. The mitogenome was a circular molecule, 15 349 base pairs (bp) long, containing 37 genes. The order and orientation of genes in the A. convolvuli mitogenome were similar to those in sequenced mitogenomes of other lepidopterans. All 13 protein-coding genes (PCGs) were initiated by ATN codons, except for the cytochrome c oxidase subunit 1 (cox1) gene, which seemed to be initiated by the codon CGA, as observed in other lepidopterans...
February 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28067539/identification-of-species-adulteration-in-traded-medicinal-plant-raw-drugs-using-dna-barcoding
#19
Stalin Nithaniyal, Sophie Lorraine Vassou, Sundar Poovitha, Balaji Raju, Madasamy Parani
Plants are the major source of therapeutic ingredients in complementary and alternative medicine (CAM). However, species adulteration in traded medicinal plant raw drugs threatens the reliability and safety of CAM. Since morphological features of medicinal plants are often not intact in the raw drugs, DNA barcoding was employed for species identification. Adulteration in 112 traded raw drugs was tested after creating a reference DNA barcode library consisting of 1452 rbcL and matK barcodes from 521 medicinal plant species...
February 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28045337/transcript-analysis-in-two-alfalfa-salt-tolerance-selected-breeding-populations-relative-to-a-non-tolerant-population
#20
M Y Gruber, J Xia, M Yu, H Steppuhn, K Wall, D Messer, A G Sharpe, S N Acharya, D S Wishart, D Johnson, D R Miller, A Taheri
With the growing limitations on arable land, alfalfa (a widely cultivated, low-input forage) is now being selected to extend cultivation into saline lands for low-cost biofeedstock purposes. Here, minerals and transcriptome profiles were compared between two new salinity-tolerant North American alfalfa breeding populations and a more salinity-sensitive western Canadian alfalfa population grown under hydroponic saline conditions. All three populations accumulated two-fold higher sodium in roots than shoots as a function of increased electrical conductivity...
February 2017: Genome Génome / Conseil National de Recherches Canada
journal
journal
29516
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"