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Genome Génome / Conseil National de Recherches Canada

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https://www.readbyqxmd.com/read/27792411/dna-barcoding-for-biosecurity-case-studies-from-the-uk-plant-protection-program
#1
Jennifer Hodgetts, Jozef C Ostojá-Starzewski, Thomas Prior, Rebecca Lawson, Jayne Hall, Neil Boonham
Since its conception, DNA barcoding has seen a rapid uptake within the research community. Nevertheless, as with many new scientific tools, progression towards the point of routine deployment within diagnostic laboratories has been slow. In this paper, we discuss the application of DNA barcoding in the Defra plant health diagnostic laboratories, where DNA barcoding is used primarily for the identification of invertebrate pests. We present a series of case studies that demonstrate the successful application of DNA barcoding but also reveal some potential limitations to expanded use...
October 28, 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27824508/the-multiple-applications-of-dna-barcodes-in-avian-evolutionary-studies
#2
Ana S Barreira, Darío A Lijtmaer, Pablo L Tubaro
DNA barcodes of birds are currently available for 41% of known species and for many different geographic areas; therefore, they are a rich data source to answer evolutionary questions. We review studies that have used DNA barcodes to investigate evolutionary processes in birds using diverse approaches. We also review studies that have investigated species in depth where taxonomy and DNA barcodes present inconsistencies. Species that showed low genetic interspecific divergence and lack of reciprocal monophyly either are the result of recent radiation and (or) hybridize, while species with large genetic splits in their COI sequences were determined to be more than one independent evolutionary unit...
October 6, 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27831816/genome-wide-analysis-of-the-dna-binding-with-one-zinc-finger-dof-transcription-factor-family-in-bananas
#3
Chen Dong, Huigang Hu, Jianghui Xie
DNA-binding with one finger (Dof) domain proteins are a multigene family of plant-specific transcription factors involved in numerous aspects of plant growth and development. In this study, we report a genome-wide search for Musa acuminata Dof (MaDof) genes and their expression profiles at different developmental stages and in response to various abiotic stresses. In addition, a complete overview of the Dof gene family in bananas is presented, including the gene structures, chromosomal locations, cis-regulatory elements, conserved protein domains, and phylogenetic inferences...
September 14, 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27753524/multi-locus-dna-barcoding-identifies-matk-as-a-suitable-marker-for-species-identification-in-hibiscus-l
#4
Sundar Poovitha, Nithaniyal Stalin, Raju Balaji, Madasamy Parani
The genus Hibiscus L. includes several taxa of medicinal value and species used for the extraction of natural dyes. These applications require the use of authentic plant materials. DNA barcoding is a molecular method for species identification, which helps in reliable authentication by using one or more DNA barcode marker. In this study, we have collected 44 accessions, representing 16 species of Hibiscus, distributed in the southern peninsular India, to evaluate the discriminatory power of the two core barcodes rbcLa and matK together with the suggested additional regions trnH-psbA and ITS2...
September 14, 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27897070/note-of-appreciation-note-de-reconnaissance
#5
(no author information available yet)
No abstract text is available yet for this article.
December 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27897069/announcement-communiqu%C3%A3
#6
(no author information available yet)
No abstract text is available yet for this article.
December 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27829307/from-barcodes-to-biomes-special-issues-from-the-6th-international-barcode-of-life-conference
#7
Sarah J Adamowicz, Frédéric J J Chain, Elizabeth L Clare, Kristy Deiner, Vlad Dincă, Manuel Elías-Gutiérrez, Axel Hausmann, Ian D Hogg, Mari Kekkonen, Darío A Lijtmaer, Amanda Naaum, Dirk Steinke, Martha Valdez-Moreno, Michelle Van der Bank, John-James Wilson, Jianping Xu
No abstract text is available yet for this article.
November 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27829306/fungal-dna-barcoding
#8
Jianping Xu
Fungi are ubiquitous in both natural and human-made environments. They play important roles in the health of plants, animals, and humans, and in broad ecosystem functions. Thus, having an efficient species-level identification system could significantly enhance our ability to treat fungal diseases and to monitor the spatial and temporal patterns of fungal distributions and migrations. DNA barcoding is a potent approach for rapid identification of fungal specimens, generating novel species hypothesis, and guiding biodiversity and ecological studies...
November 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27829305/barcodes-to-biomes-codes-barres-pour-biomes
#9
(no author information available yet)
No abstract text is available yet for this article.
November 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27673501/dna-barcoding-as-a-useful-tool-in-the-systematic-study-of-wild-bees-of-the-tribe-augochlorini-hymenoptera-halictidae
#10
Rocío Ana González-Vaquero, Arturo Roig-Alsina, Laurence Packer
Special care is needed in the delimitation and identification of halictid bee species, which are renowned for being morphologically monotonous. Corynura Spinola and Halictillus Moure (Halictidae: Augochlorini) contain species that are key elements in southern South American ecosystems. These bees are very difficult to identify due to close morphological similarity among species and high sexual dimorphism. We analyzed 170 barcode-compliant COI sequences from 19 species. DNA barcodes were useful to confirm gender associations and to detect two new cryptic species...
October 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27673405/dna-barcode-variability-and-host-plant-usage-of-fruit-flies-diptera-tephritidae-in-thailand
#11
Chonticha Kunprom, Pairot Pramual
The objectives of this study were to examine the genetic variation in fruit flies (Diptera: Tephritidae) in Thailand and to test the efficiency of the mitochondrial cytochrome c oxidase subunit I (COI) barcoding region for species-level identification. Twelve fruit fly species were collected from 24 host plant species of 13 families. The number of host plant species for each fruit fly species ranged between 1 and 11, with Bactrocera correcta found in the most diverse host plants. A total of 123 COI sequences were obtained from these fruit fly species...
October 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27653246/genome-wide-identification-and-expression-analysis-of-the-apple-asr-gene-family-in-response-to-alternaria-alternata-f-sp-mali
#12
Kaihui Huang, Yan Zhong, Yingjun Li, Dan Zheng, Zong-Ming Cheng
The ABA/water stress/ripening-induced (ASR) gene family exists universally in higher plants, and many ASR genes are up-regulated during periods of environmental stress and fruit ripening. Although a considerable amount of research has been performed investigating ASR gene response to abiotic stresses, relatively little is known about their roles in response to biotic stresses. In this report, we identified five ASR genes in apple (Malus × domestica) and explored their phylogenetic relationship, duplication events, and selective pressure...
October 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27643679/r-l-a-double-reporter-mouse-line-that-expresses-luciferase-gene-upon-cre-mediated-excision-followed-by-inactivation-of-mrfp-expression
#13
Junshuang Jia, Xiaolin Lin, Xia Lin, Taoyan Lin, Bangzhu Chen, Weichao Hao, Yushuang Cheng, Yu Liu, Meijuan Dian, Kaitai Yao, Dong Xiao, Weiwang Gu
The Cre/loxP system has become an important tool for the conditional gene knockout and conditional gene expression in genetically engineered mice. The applications of this system depend on transgenic reporter mouse lines that provide Cre recombinase activity with a defined cell type-, tissue-, or developmental stage-specificity. To develop a sensitive assay for monitoring Cre-mediated DNA excisions in mice, we generated Cre-mediated excision reporter mice, designated R/L mice (R/L: mRFP(monomeric red fluorescent protein)/luciferase), express mRFP throughout embryonic development and adult stages, while Cre-mediated excision deletes a loxP-flanked mRFP reporter gene and STOP sequence, thereby activating the expression of the second reporter gene luciferase, as assayed by in vivo and ex vivo bioluminescence imaging...
October 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27643577/cytological-and-molecular-analysis-of-wheat-agropyron-cristatum-translocation-lines-with-6p-chromosome-fragments-conferring-superior-agronomic-traits-in-common-wheat
#14
Liqiang Song, Yuqing Lu, Jinpeng Zhang, Cuili Pan, Xinming Yang, Xiuquan Li, Weihua Liu, Lihui Li
Agropyron cristatum (2n = 4x = 28, PPPP) is a wild relative of common wheat and confers several desirable agronomic traits to wheat, such as high grain number per spike and enhanced resistance to certain diseases. Development of wheat - A. cristatum 6P translocation lines facilitates its utilization in wheat improvement. In this study, 26 wheat - A. cristatum 6P translocation lines were characterized by in situ hybridization (ISH) and 6P-specific sequence-tagged-site (STS) markers. These translocation lines carried different 6P chromosomal segments, which covered the whole 6P chromosome...
October 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27608265/is-nematocharax-actinopterygii-characiformes-a-monotypic-fish-genus
#15
Silvia Britto Barreto, Lorena Andrade Nunes, André Teixeira da Silva, Ricardo Jucá-Chagas, Débora Diniz, Iracilda Sampaio, Horacio Schneider, Paulo Roberto Antunes de Mello Affonso
The combination of DNA barcodes and geometric morphometrics is useful to discriminate taxonomically controversial species, providing more precise estimates of biodiversity. Therefore, our goal was to assess the genetic and morphometric diversity in Nematocharax, a controversial monotypic and sexually dimorphic genus of Neotropical fish, based on sequencing of cytochrome c oxidase subunit I (COI) and morphometric analyses in seven populations of N. venustus from coastal rivers in Brazil. The average pairwise intrapopulation divergence in COI ranged from 0 to 2...
October 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27603265/bcgtree-automatized-phylogenetic-tree-building-from-bacterial-core-genomes
#16
Markus J Ankenbrand, Alexander Keller
The need for multi-gene analyses in scientific fields such as phylogenetics and DNA barcoding has increased in recent years. In particular, these approaches are increasingly important for differentiating bacterial species, where reliance on the standard 16S rDNA marker can result in poor resolution. Additionally, the assembly of bacterial genomes has become a standard task due to advances in next-generation sequencing technologies. We created a bioinformatic pipeline, bcgTree, which uses assembled bacterial genomes either from databases or own sequencing results from the user to reconstruct their phylogenetic history...
October 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27549861/genetics-and-molecular-mapping-of-resistance-to-plasmodiophora-brassicae-pathotypes-2-3-5-6-and-8-in-rutabaga-brassica-napus-var-napobrassica
#17
Muhammad Jakir Hasan, Habibur Rahman
Clubroot disease, caused by Plasmodiophora brassicae, is a threat to the production of Brassica crops including oilseed B. napus. In Canada, several pathotypes of this pathogen, such as pathotypes 2, 3, 5, 6, and 8, were identified, and resistance to these pathotypes was found in a rutabaga (B. napus var. napobrassica) genotype. In this paper, we report the genetic basis and molecular mapping of this resistance by use of F2, backcross (BC1), and doubled haploid (DH) populations generated from crossing of this rutabaga line to a susceptible spring B...
October 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27767337/barcoding-the-food-chain-from-sanger-to-high-throughput-sequencing
#18
Joanne E Littlefair, Elizabeth L Clare
Society faces the complex challenge of supporting biodiversity and ecosystem functioning, while ensuring food security by providing safe traceable food through an ever-more-complex global food chain. The increase in human mobility brings the added threat of pests, parasites, and invaders that further complicate our agro-industrial efforts. DNA barcoding technologies allow researchers to identify both individual species, and, when combined with universal primers and high-throughput sequencing techniques, the diversity within mixed samples (metabarcoding)...
September 2, 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27611699/from-barcodes-to-biomes-special-issues-from-the-6th-international-barcode-of-life-conference
#19
Sarah J Adamowicz, Frédéric J J Chain, Elizabeth L Clare, Kristy Deiner, Vlad Dincă, Manuel Elías-Gutiérrez, Axel Hausmann, Ian D Hogg, Mari Kekkonen, Darío A Lijtmaer, Amanda Naaum, Dirk Steinke, Martha Valdez-Moreno, Michelle Van der Bank, John-James Wilson, Jianping Xu
No abstract text is available yet for this article.
September 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/27611698/barcodes-to-biomes-codes-barres-pour-biomes
#20
(no author information available yet)
No abstract text is available yet for this article.
September 2016: Genome Génome / Conseil National de Recherches Canada
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