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Parasitology Research

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https://www.readbyqxmd.com/read/28101647/a-real-time-pcr-approach-to-identify-anthelmintic-resistant-nematodes-in-sheep-farms
#1
M Milhes, M Guillerm, M Robin, M Eichstadt, C Roy, C Grisez, F Prévot, E Liénard, E Bouhsira, M Franc, P Jacquiet
Resistance to fenbendazole, ivermectin, and moxidectin was explored by a fecal egg count reduction test in four meat sheep flocks in southwestern France where anthelmintic resistance was suspected. The FECR test results of the present study confirmed the presence of benzimidazole resistance in three out of the four farms and the presence of ivermectin resistance in one flock. In addition, a suspicion of moxidectin resistance was shown in this latter farm. Both conventional morphological and molecular identifications were performed on larval cultures before and after the treatment in the studied farms...
January 18, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28083657/peripheral-blood-cd4-cd25-regulatory-t-cells-in-alcoholic-patients-with-strongyloides-stercoralis-infection
#2
Steveen Rios Ribeiro, Luciana Polaco Covre, Lorenzzo Lyrio Stringari, Maria da Penha Zago-Gomes, Daniel Cláudio Oliveira Gomes, Fausto Edmundo Lima Pereira
An increased number of regulatory T (Treg) cells has been reported in patients with HTLV-1 and Strongyloides stercoralis co-infection, suggesting the contribution of these cells to worm survival. As Strongyloides infections have been found to be highly prevalent in chronic alcoholics, we investigated the effect of abusive ethanol ingestion on the induction of Treg cells in alcoholic patients with Strongyloides infection. Treg cells were assessed by flow cytometry in the peripheral blood of 12 healthy non-alcoholic (control) and 14 alcoholic patients (alcoholic) without Strongyloides infection and five non-alcoholics (controlSs) and five chronic alcoholics (alcoholSs) with Strongyloides infection...
January 12, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28074315/the-roundworm-strongyloides-stercoralis-in-children-dogs-and-soil-inside-and-outside-a-segregated-settlement-in-eastern-slovakia-frequent-but-hardly-detectable-parasite
#3
G Štrkolcová, M Goldová, E Bocková, J Mojžišová
A comparative study was carried out to evaluate the Strongyloides stercoralis infections in children and dogs inside and outside the segregated settlement in Medzev, Eastern Slovakia, and a survey of the soil within the settlement was included. Applying the Koga agar plate (KAP) culture method and microscopy examination of stool samples collected from 60 Roma and 21 nonRoma children, no larvae of S. stercoralis were detected but eggs of three nematodes (Ascaris lumbricoides, Trichuris trichiura, and Enterobius vermicularis) and cysts of two protozoan endoparasites (Giardia duodenalis and Cryptosporidium spp...
January 10, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28070635/incidence-of-three-kudoa-spp-k-neothunni-k-hexapunctata-and-k-thunni-myxosporea-multivalvulida-in-thunnus-tunas-distributed-in-the-western-pacific-ocean
#4
Akihiro Kasai, Hideaki Tsuduki, Lea Angsinco Jimenez, Ying-Chun Li, Shuhei Tanaka, Hiroshi Sato
A variety of tunas of the genus Thunnus are consumed daily in Japan as sliced raw fish (sashimi and sushi). The consumption of fresh sliced raw fish, i.e., unfrozen or uncooked, can sometimes cause food poisoning that is manifested by transient diarrhea and vomiting for a single day. One of the causes of this type of food poisoning has been identified as live Kudoa septempunctata (Myxosporea: Multivalvulida) in the olive flounder (Paralichthys olivaceus). Furthermore, raw slices of fresh tunas are highly suspected to be a possible causative fish of similar food poisoning in Japan...
January 9, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28066871/cysteine-protease-inhibitor-of-schistosoma-japonicum-a-parasite-derived-negative-immunoregulatory-factor
#5
Lin Chen, Baohua He, Wei Hou, Li He
Studies have shown that cysteine protease inhibitors from some parasites have immunosuppressive effects on the host. We previously have cloned a novel cysteine protease inhibitor from Schistosoma japonicum and purified its recombinant version (protein named rSj-C). Its possible inhibitory effect on the host immune response has not been described.This study shows that rSj-C inhibits lysosomal cysteine protease of murine dendritic cells (DCs). After DCs were incubated with rSj-C and then with soluble adult worm antigen (AWA) of S...
January 9, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28063008/association-study-of-demodex-bacteria-and-facial-dermatoses-based-on-dgge-technique
#6
YaE Zhao, Fan Yang, RuiLing Wang, DongLing Niu, Xin Mu, Rui Yang, Li Hu
The role of bacteria is unclear in the facial skin lesions caused by Demodex. To shed some light on this issue, we conducted a case-control study comparing cases with facial dermatoses with controls with healthy skin using denaturing gradient gel electrophoresis (DGGE) technique. The bacterial diversity, composition, and principal component were analyzed for Demodex bacteria and the matched facial skin bacteria. The result of mite examination showed that all 33 cases were infected with Demodex folliculorum (D...
January 6, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28058537/in-vivo-efficacy-of-a-biotherapic-and-eugenol-formulation-against-rhipicephalus-microplus
#7
Paula Pimentel Valente, Gustavo Henrique Ferreira Abreu Moreira, Matheus Ferreira Serafini, Elias Jorge Facury-Filho, Antônio Último Carvalho, André Augusto Gomes Faraco, Rachel Oliveira Castilho, Múcio Flávio Barbosa Ribeiro
The control of Rhipicephalus microplus is essential to prevent cattle discomfort and economic losses. However, increased resistance and acaricides inefficiency lead producers to adopt strategies that could result in the accumulation of chemical residues in meat and milk with possibilities of poisoning in animals and people. This scenario demonstrates the necessity of research into the identification of novel, effective and environmentally safe therapeutic options for cattle tick control. The objectives of this study were to develop and assess the efficacy of R...
January 5, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28058536/diversity-of-cryptosporidium-species-occurring-in-sheep-and-goat-breeds-reared-in-poland
#8
Agnieszka Kaupke, Mirosław M Michalski, Artur Rzeżutka
The aim of this study was molecular identification of Cryptosporidium species and assessment of their prevalence in different breeds of sheep and goat reared in Poland. In addition, the relationship between animal age, breed type, and the frequency of Cryptosporidium infections was determined. Fecal samples from 234 lambs and 105 goat kids aged up to 9 weeks, representing 24 breeds and their cross-breeds were collected from 71 small ruminant farms across Poland. The identification of Cryptosporidium species was performed at the 18 SSU ribosomal RNA (rRNA) and COWP loci followed by subtyping of C...
January 5, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28058535/molecular-and-structural-characteristics-of-multidrug-resistance-associated-protein-7-in-chinese-liver-fluke-clonorchis-sinensis
#9
Fuhong Dai, Won Gi Yoo, Ji-Yun Lee, Yanyan Lu, Jhang Ho Pak, Woon-Mok Sohn, Sung-Jong Hong
Multidrug resistance-associated protein 7 (MRP7, ABCC10) is a C subfamily member of the ATP-binding cassette (ABC) superfamily. MRP7 is a lipophilic anion transporter that pumps endogenous and xenobiotic substrates from the cytoplasm to the extracellular milieu. Here, we cloned and characterized CsMRP7 as a novel ABC transporter from the Chinese liver fluke, Clonorchis sinensis. Full-length cDNA of CsMRP7 was 5174 nt, encoded 1636 amino acids (aa), and harbored a 147-bp 5'-untranslated region (5'-UTR) and 116-bp 3'-UTR...
January 5, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28054180/status-of-babesiosis-among-domestic-herbivores-in-iran-a-systematic-review-and-meta-analysis
#10
REVIEW
Mousa Motavalli Haghi, Fariborz Etemadifar, Mahdi Fakhar, Saeed Hosseini Teshnizi, Masoud Soosaraei, Azar Shokri, Atta Hajihasani, Hamed Mashhadi
Babesiosis is a protozoal disease caused by Babesia spp. in mammals and humans worldwide. It is one of the most important tick-borne diseases, which affects livestock productions, reproductions, and accordingly failing economy. In this, systematic review and meta-analysis, study, the prevalence of babesiosis among domestic herbivores in Iran, between 1998 and 2015, was methodically reviewed. Nine databases including five English and four Persian databases were explored. A total of 49 articles, as regards the examination of 13,547 sheep, 1920 goats, 7167 cattle, and 940 horses, corresponding to prevalence of babesiosis from different regions of Iran were gathered for our qualifying criteria...
January 4, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28054179/activity-of-culicoides-spp-diptera-ceratopogonidae-inside-and-outside-of-livestock-stables-in-late-winter-and-spring
#11
Daniela Kameke, Helge Kampen, Doreen Walther
Culicoides Latreille, 1809 midge species are the putative vectors of Bluetongue virus (BTV) and Schmallenberg virus (SBV) in Europe. To gain a better understanding of the epidemiology of the diseases, basic knowledge about the overwintering of the vectors is needed. Therefore, we investigated culicoid activity in relation to air temperature at livestock stables during late winter and spring season. Ceratopogonids were captured weekly indoors and outdoors on three cattle farms, three horse farms and one sheep farm in the federal state of Brandenburg, Germany between January and May, 2015 by BG-Sentinel UV-light suction traps...
January 4, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28039498/the-p38-mapk-inhibitor-sb203580-inhibits-cell-invasion-by-neospora-caninum
#12
Xiaoxia Jin, Pengtao Gong, Guojiang Li, Xichen Zhang, Jianhua Li
The Apicomplexan parasite Neospora caninum is an obligate intracellular parasitic protozoan. It can cause severe diseases in a number of animals throughout the world. Infection with N. caninum leads to abortions in pregnant animals and neuromuscular disorders of newborns which cause great economic losses to animal husbandry. However, the mechanism of cell invasion by N. caninum is still unclear. This paper aims to investigate the impact of SB203580, a p38 MAPK inhibitor, on host cell invasion by N. caninum...
December 30, 2016: Parasitology Research
https://www.readbyqxmd.com/read/28039497/synergistic-effects-of-fenbendazole-and-metronidazole-against-giardia-muris-in-swiss-mice-naturally-infected
#13
Renata Coltro Bezagio, Cristiane Maria Colli, Liara Izabela Lopes Romera, Érika Cristina Ferreira, Ana Lúcia Falavigna-Guilherme, Mônica Lúcia Gomes
In this study were proposed different protocols for the treatment of mice naturally infected with Giardia muris. Male Swiss mice were divided into seven groups, with five animals each, in a blind, controlled, randomized by drawing lots and once-repeated experiment. Parasite detection and cure control were performed using the Faust method and search by trophozoites in the intestinal mucosa. Clinical parameters (weight, water and feed consumption, elimination of excreta, aspect of the fur and feces) were also evaluated...
December 30, 2016: Parasitology Research
https://www.readbyqxmd.com/read/28032300/genotypic-and-phenotypic-evaluation-for-benzimidazole-resistance-or-susceptibility-in-haemonchus-contortus-isolates
#14
Waleed M Arafa, Patricia J Holman, Thomas M Craig
Haemonchus contortus isolates were evaluated for benzimidazole (BZ) resistance or susceptibility by allele-specific PCR based on β-tubulin isotype 1 gene polymorphisms at the F167Y, E198A, and F200Y sites. Two isolates, one presumed susceptible from wild pronghorn antelope (PH) and one known to be resistant from goats (VM), were also assayed phenotypically for BZ resistance or susceptibility in the larval development assay (Drenchrite®). The BZ EC50 was 0.198 μM (intermediate between susceptible and weak resistant) for PH with critical well 5 (intermediate between susceptible and weak resistant) and 1...
December 28, 2016: Parasitology Research
https://www.readbyqxmd.com/read/28028631/molecular-detection-and-genetic-diversity-of-bovine-babesia-spp-theileria-orientalis-and-anaplasma-marginale-in-beef-cattle-in-thailand
#15
Charoonluk Jirapattharasate, Paul Franck Adjou Moumouni, Shinuo Cao, Aiko Iguchi, Mingming Liu, Guanbo Wang, Mo Zhou, Patrick Vudriko, Artemis Efstratiou, Tanasak Changbunjong, Sivapong Sungpradit, Parntep Ratanakorn, Walasinee Moonarmart, Poonyapat Sedwisai, Thekhawet Weluwanarak, Witsanu Wongsawang, Hiroshi Suzuki, Xuenan Xuan
Babesia spp., Theileria orientalis, and Anaplasma marginale are significant tick-borne pathogens that affect the health and productivity of cattle in tropical and subtropical areas. In this study, we used PCR to detect the presence of Babesia bovis, Babesia bigemina, and T. orientalis in 279 beef cattle from Western Thailand and A. marginale in 608 beef cattle from the north, northeastern, and western regions. The PCRs were performed using species-specific primers based on the B. bovis spherical body protein 2 (BboSBP2), B...
December 27, 2016: Parasitology Research
https://www.readbyqxmd.com/read/28028630/characterizations-and-bioactivities-of-abendazole-sulfoxide-loaded-thermo-sensitive-hydrogel
#16
Yi Feng, Fen Wang, Xue-Wei Zhang, Harshit Bhutani, Bin Ye
Albendazole (ABZ), a widely used anthelmintic, attributes its primary metabolite-albendazole sulfoxide (ABZSO)-as an effective agent against helminthes. For a purpose of long-lasting releasing ABZSO in a special lesion, the present study successfully manufactured ABZSO-loaded thermo-sensitive hydrogel, which was proved by FTIR and (1)H NMR, in the interim; in vitro and in vivo behaviors of the thermo-sensitive hydrogel containing ABZSO were studied too. The in vivo pharmacokinetics parameters indicated ABZSO-loaded hydrogel as a better choice for sustained release compared with simple ABZSO...
December 27, 2016: Parasitology Research
https://www.readbyqxmd.com/read/28028629/histologic-parameters-for-detecting-multiple-blood-meals-in-aedes-albopictus-skuse-diptera-culicidae-during-a-single-gonotrophic-cycle
#17
Abeer S Yamany, Fatma K Adham, Heinz Mehlhorn
The histologic technique was used for its usefulness in detecting multiple blood feedings in Aedes albopictus during a single gonotrophic cycle. In a laboratory study, in which 200 blood-engorged Ae. albopictus mosquito females during a 3-day period were examined, 69% (138/200) of these females imbibed two blood meals when the interval between meals was from 8 to 48 h and the time between the second blood meal and fixation ranged from 0 to 32 h. At the interval outside this range, only 23% of the multiple meals were detected when using histologic parameters...
December 27, 2016: Parasitology Research
https://www.readbyqxmd.com/read/28028628/risk-of-drug-resistance-in-plasmodium-falciparum-malaria-therapy-a-systematic-review-and-meta-analysis
#18
Li-Juan Zhou, Jing Xia, Hai-Xia Wei, Xiao-Jun Liu, Hong-Juan Peng
Plasmodium falciparum is responsible for the vast majority of the morbidity and mortality associated with malaria infection globally. Although a number of studies have reported the emergence of drug resistance in different therapies for P. falciparum infection, the degree of the drug resistance in different antimalarials is still unclear. This research investigated the risk of drug resistance in the therapies with different medications based on meta-analyses. Relevant original randomized control trials (RCTs) were searched in all available electronic databases...
December 27, 2016: Parasitology Research
https://www.readbyqxmd.com/read/28019000/avian-and-simian-malaria-do-they-have-a-cancer-connection
#19
REVIEW
Martin Ward, Giovanni Benelli
It has been claimed that infectious agents transmitted by mosquitoes (Diptera: Culicidae) may have a greater connection to cancer then hitherto supposed and that the immune system struggles to recognize and fight some of these infectious agents. One of the claims made is that there is a connection between human malaria and brain cancers in the USA. However, the USA declared itself free of human malaria in the last century, yet cancer incidences remain high, suggesting any overall cancer connection is slight...
December 26, 2016: Parasitology Research
https://www.readbyqxmd.com/read/28018999/new-approach-to-molecular-characterization-of-paramphistomum-cervi-and-carmyerius-gregarius-and-comparative-analyses-with-selected-trematodes
#20
Nasr M El-Bahy, Eman K Bazh, Amer R Abdel Azizn, Ahmed Elkhtam
The two ruminant parasites, Paramphistomum cervi and Carmyerius gregarius, were collected from fresh-slaughtered native cattle at local abattoirs in Sadat district, Menoufia province and identified morphologically, then molecularly by sequencing the nucleotides of 18S ribosomal RNA gene (18S rRNA). The nucleotide sequences of the two isolates were 456 (P. cervi) and 401 bases for (C. gregarius). The data were used along with those of several other helminth species from the GenBank to identify these two species genetically...
December 26, 2016: Parasitology Research
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