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Parasitology Research

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https://www.readbyqxmd.com/read/28821960/comparative-analysis-of-germ-cells-and-dna-of-the-genus-amblyomma-adding-new-data-on-amblyomma-maculatum-and-amblyomma-ovale-species-acari-ixodidae
#1
Fredy Arvey Rivera-Páez, Bruno Rodrigues Sampieri, Marcelo Bahia Labruna, Renata da Silva Matos, Thiago Fernandes Martins, Maria Izabel Camargo-Mathias
Among tick species, members of the subfamily Amblyomminae have received special attention, since they serve as vectors for pathogens such as Rickettsia spp. and display cryptic species complexes that make their taxonomical classification challenging. Amblyomma ovale, Amblyomma maculatum, and other species of the genus Amblyomma have shown a long history of taxonomic controversies. Spermiotaxonomy has proved to be a valuable tool in the solution of systematic conflicts in Metazoa that can aid molecular and external morphological analyses in ticks and, overall, provide more robust analyses and results...
August 18, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28815299/morphological-and-phylogenetic-analysis-of-a-microsporidium-nosema-sp-isolated-from-rice-stem-borer-chilo-suppressalis-walker-lepidoptera-pyralidae
#2
Dongxu Xing, Qiong Yang, Sentai Liao, Lanzhi Han, Qingrong Li, Chaoyi Zhao, Yang Xiao, Mingqiang Ye
A new microsporidium was isolated from Chilo suppressalis (Walker) (Lepidoptera: Pyralidae), one of the most important rice pests in China. The morphology and molecular systematics of this novel microsporidium were described in this study. The spores were long oval and measured 3.17 × 1.64 μm on fresh smears. Ultrastructure of the spores was characteristic for the genus Nosema: a diplokaryon, 10-12 polar filament coils of the same type, and posterior vacuole. Small subunit rRNA gene sequence data and phylogenetic analysis further confirmed that the microsporidian species from C...
August 16, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28803388/genetic-diversity-of-the-human-head-lice-pediculus-humanus-capitis-among-primary-school-girls-in-saudi-arabia-with-reference-to-their-prevalence
#3
Sarah A Al-Shahrani, Reem A Alajmi, Tahany H Ayaad, Mohammed A Al-Shahrani, El-Sayed H Shaurub
The present work aimed at investigating the genetic diversity of the head louse Pediculus humanus capitis (P. humanus capitis) among infested primary school girls at Bisha governorate, Saudi Arabia, based on the sequence of mitochondrial cytochrome b (mt cyt b) gene of 121 P. humanus capitis adults. Additionally, the prevalence of pediculosis capitis was surveyed. The results of sequencing were compared with the sequence of human head lice that are genotyped previously. Phylogenetic tree analysis showed the presence of 100% identity (n = 26) of louse specimens with clade A (prevalent worldwide) of the GenBank data base...
August 13, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28803361/toxoplasma-gondii-and-neospora-caninum-induce-different-host-cell-responses-at-proteome-wide-phosphorylation-events-a-step-forward-for-uncovering-the-biological-differences-between-these-closely-related-parasites
#4
Mariwan M M Al-Bajalan, Dong Xia, Stuart Armstrong, Nadine Randle, Jonathan M Wastling
Toxoplasma gondii and Neospora caninum are closely related intracellular protozoan parasites and tissue cyst-forming Coccidia of the phylum Apicomplexa. There are remarkable similarities between the morphology, genomes and transcriptomes of both parasites. Toxoplasma is zoonotic, with a wide host range and is mainly transmitted horizontally between its definitive host, the cat, and its intermediate hosts. Neospora causes disease within a narrow host range and with reduced virulence potential to the hosts. The dog is the definitive host of Neospora and its epidemiology in cattle mainly depends on vertical transmission...
August 12, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28803354/facultative-myiasis-of-domestic-cats-by-sarcophaga-argyrostoma-diptera-sarcophagidae-calliphora-vicina-and-lucilia-sericata-diptera-calliphoridae-in-northern-italy
#5
Marco Pezzi, Daniel Whitmore, Teresa Bonacci, Carlo Nicola Francesco Del Zingaro, Milvia Chicca, Massimo Lanfredi, Marilena Leis
We describe five cases of myiasis of domestic cats, Felis silvestris catus L. (Carnivora: Felidae), reported in 2016 in northern Italy and caused by three Diptera species: Sarcophaga argyrostoma (Robineau-Desvoidy) (Sarcophagidae), Calliphora vicina Robineau-Desvoidy and Lucilia sericata (Meigen) (Calliphoridae). Three were cases of traumatic myiasis, one by S. argyrostoma and two by L. sericata, one was a case of auricular myiasis by C. vicina and one was a case of ophthalmomyiasis caused by an association of L...
August 12, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28801696/clonorchis-sinensis-infection-and-co-infection-with-the-hepatitis-b-virus-are-important-factors-associated-with-cholangiocarcinoma-and-hepatocellular-carcinoma
#6
Yunliang Shi, Zhihua Jiang, Yichao Yang, Peiqiu Zheng, Haiyan Wei, Yuan Lin, Guoli Lv, Qingli Yang
To evaluate the contributions of Clonorchis sinensis and hepatitis B virus to the development of cholangiocarcinoma (ICC) and hepatocellular carcinoma (HCC), C. sinensis and hepatitis B virus infections in 20 clinical liver cancer cases from a C. sinensis- and hepatitis B virus-epidemic region were detected. Eight cases of ICC, 11 cases of HCC and one mixed ICC and HCC case were verified by CT, pathological section and (or) observations during surgery. The C. sinensis infection was detected by stool microscopy and ELISA, and the worms and eggs found during surgery and in pathological sections also allowed for diagnoses...
August 12, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28799088/metazoan-parasites-from-odontocetes-off-new-zealand-new-records
#7
Kristina Lehnert, Haseeb Randhawa, Robert Poulin
Information about the parasite fauna of spectacled porpoises and cetaceans from New Zealand waters in general is scarce. This study takes advantage of material archived in collections of the Otago Museum in Dunedin and Massey University in Auckland, sampled from cetacean species found stranded along the New Zealand coastline between 2007 and 2014. Parasites from seven species of cetaceans (spectacled porpoise, Phocoena dioptrica (n = 2 individuals examined); pygmy sperm whale (n = 1); long-finned pilot whale, Globicephala melas (n = 1); Risso's dolphin, Grampus griseus (n = 1); short-beaked common dolphin, Delphinus delphis (n = 7); striped dolphin, Stenella coeruleoalba (n = 3) and dusky dolphin, Lagenorhynchus obscurus (n = 2)) from the respiratory and gastro-intestinal tract, cranial sinus, liver, urogenital and mammary tract, fascia and blubber were investigated...
August 10, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28795223/high-resolution-melting-pcr-assay-applicable-for-diagnostics-and-screening-studies-allowing-detection-and-differentiation-of-several-babesia-spp-infecting-humans-and-animals
#8
Wioletta Rozej-Bielicka, Aleksander Masny, Elzbieta Golab
The goal of the study was to design a single tube PCR test for detection and differentiation of Babesia species in DNA samples obtained from diverse biological materials. A multiplex, single tube PCR test was designed for amplification of approximately 400 bp region of the Babesia 18S rRNA gene. Universal primers were designed to match DNA of multiple Babesia spp. and to have low levels of similarity to DNA sequences of other intracellular protozoa and Babesia hosts. The PCR products amplified from Babesia DNA isolated from human, dog, rodent, deer, and tick samples were subjected to high-resolution melting analysis for Babesia species identification...
August 10, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28795224/extrusion-of-contracaecum-osculatum-nematode-larvae-from-the-liver-of-cod-gadus-morhua
#9
S Zuo, L Barlaup, A Mohammadkarami, A Al-Jubury, D Chen, P W Kania, K Buchmann
Baltic cod livers have during recent years been found increasingly and heavily infected with third-stage larvae of Contracaecum osculatum. The infections are associated with an increasing population of grey seals which are final hosts for the parasite. Heavy worm burdens challenge utilization and safety of the fish liver products, and technological solutions for removal of worms are highly needed. We investigated the attachment of the worm larvae in liver tissue by use of histochemical techniques and found that the cod host encapsulates the worm larvae in layers of host cells (macrophages, fibroblasts) supported by enclosures of collagen and calcium...
August 9, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28785847/efficacy-and-tolerability-of-treatment-with-single-doses-of-diethylcarbamazine-dec-and-dec-plus-albendazole-abz-for-three-consecutive-years-in-lymphatic-filariasis-a-field-study-in-india
#10
Nilima A Kshirsagar, N J Gogtay, B S Garg, P R Deshmukh, D D Rajgor, V S Kadam, P A Thakur, A Gupta, N S Ingole, J K Lazdins-Helds
Lymphatic filariasis (LF) affects 73 countries, causes morbidity and impedes socioeconomic development. We had found no difference in safety and micro (Mf) and macro filarial action of single-dose diethylcarbamazine (DEC) and DEC + albendazole (ABZ) in an F01 study done in India (year 2000). There was a programmatic need to evaluate safety and efficacy of multiple annual treatments (F02). Subjects (155) from the F01 study, meeting inclusion-exclusion criteria, were enrolled in F02 and treated with further two annual doses of DEC or DEC + ABZ...
August 7, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28785846/re-description-of-a-genetically-typed-single-oocyst-line-of-the-turkey-coccidium-eimeria-dispersa-tyzzer-1929
#11
S El-Sherry, M E Ogedengbe, M A Hafeez, M Sayf-Al-Din, N Gad, J R Barta
The Briston strain of Eimeria dispersa Tyzzer, 1929 was isolated originally from a commercial turkey flock from Briston, Norfolk, UK. A single oocyst-derived line of E. dispersa was propagated and used to re-describe biological and morphological features of E. dispersa in the turkey. Oocysts of the Briston strain measured 26 ± 1.1 μm (24-28) by 21 ± 1 μm (19-23); these were larger than oocysts described originally by Tyzzer in 1929 (22.75 by 18.84 μm) but within dimensions (26.07 by 21.04 μm) reported by Hawkins (1952) in his description of E...
August 7, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28785845/the-unique-uterine-structure-of-the-basal-monogenean-chimaericola-leptogaster-monogenea-polyopisthocotylea-an-ectoparasite-of-the-relictual-holocephalan-fish-chimaera-monstrosa
#12
Larisa G Poddubnaya, Willy Hemmingsen, David I Gibson
Ultrastructural studies of the monogenean uterus are few in number and no non-polystomatid polyopisthocotyleans have been investigated. The uterus of Chimaericola leptogaster, a basal polyopisthocotylean monogenean, has several unusual features, including six reflexed loops comprising four ascending and three descending, longitudinally oriented, linear sections. At the ultrastructural level, three readily distinguishable uterine regions and other distinctive characteristics are apparent. One novel feature occurs in the proximal uterus, where the lining forms a so-called 'single-layered multi-rowed cellular epithelium', which includes two types of cells, tall (ca...
August 7, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28779214/myxobolus-sp-and-henneguya-sp-cnidaria-myxobolidae-natural-co-infection-in-the-kidney-of-piaractus-mesopotamicus-characiformes-serrasalmidae
#13
Wilson Gómez Manrique, Mayra Araguaia Pereira Figueiredo, Marco Antonio de Andrade Belo, Maurício Laterça Martins, Kálmán Molnár
This study evaluated the myxozoan infection and histopathology of the kidney of freshwater fish Piaractus mesopotamicus from intensive fish farming in Brazil. A total of 55 fish were examined for myxozoan infection. Infected organs were processed by usual histology and stained with hematoxylin-eosin (H&E) and Ziehl-Neelsen (ZN). From the total of 55 fish analyzed, 47 (85.45%) presented myxospores, being 9.09% (5/55) only with Myxobolus sp., 5.45% (3/55) only with Henneguya sp., and 70.91% (39/55) presenting both parasites...
August 5, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28776228/symptoms-and-risk-factors-of-cryptosporidium-hominis-infection-in-children-data-from-a-large-waterborne-outbreak-in-sweden
#14
Sara Adler, Micael Widerström, Johan Lindh, Mikael Lilja
Cryptosporidium is a major cause of diarrheal disease worldwide. In developing countries, this infection is endemic and in children, associated with growth faltering and cognitive function deficits, with the most severe impact on those aged <2 years. Little has been reported about symptoms and risk factors for children in industrialized countries, although the disease incidence is increasing in such regions. In November 2010, a large waterborne outbreak of C. hominis occurred in the city of Östersund in Sweden...
August 4, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28776227/seroprevalence-and-risk-factors-associated-with-ehrlichia-canis-anaplasma-spp-borrelia-burgdorferi-sensu-lato-and-d-immitis-in-hunting-dogs-from-southern-italy
#15
Diego Piantedosi, Benedetto Neola, Nicola D'Alessio, Francesca Di Prisco, Mario Santoro, Laura Pacifico, Giovanni Sgroi, Luigi Auletta, Jesse Buch, Ramaswamy Chandrashekar, Edward B Breitschwerdt, Vincenzo Veneziano
Canine vector-borne diseases (CVBDs) are caused by a range of pathogens transmitted to dogs by arthropods. The present study investigates Ehrlichia canis, Anaplasma spp., Borrelia burgdorferi sensu lato, and Dirofilaria immitis seroprevalences in hunting dogs from southern Italy. Dogs (no. 1335) were tested using a commercial in-clinic enzyme-linked immunosorbent assay kit. Odds ratios (ORs) were calculated by logistic regression analysis to identify risk factors. Overall, 138/1335 dogs (10.3%) were seroreactive to at least one CVBD pathogen...
August 4, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28776226/a-screen-of-pharmaceutical-drugs-for-their-ability-to-cause-short-term-morbidity-and-mortality-in-the-common-bed-bug-cimex-lectularius-l
#16
Johnathan M Sheele, Gale E Ridge, Wenjing Du, Nikhil Mallipeddi, Mayur Vallabhaneni
The common bed bug, Cimex lectularius L., is a hematophagous ectoparasite that preferentially feeds on humans. Pharmaceuticals present in a person's blood may adversely affect C. lectularius when it feeds. We fed >10,000 C. lectularius on blood samples containing more than 400 different drug doses and drug combinations using an in vitro feeding system to determine insect mortality. The majority of drug doses approximated the peak plasma concentration in humans taking those drugs. Twenty-one drugs were found to cause >17% 12-14-day mortality compared to 8...
August 3, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28766154/the-comparative-effect-of-chlorine-and-huwa-san-as-disinfecting-agents-on-biomphalaria-alexandrina-snails-and-free-larval-stages-of-schistosoma-mansoni
#17
Sara S M Sayed, Marwa T A Abdel-Wareth
Many chemicals are applied for disinfecting purposes. Although chlorination is the most common procedure, it has many drawbacks. So there becomes an urgent need for new chemicals which are both effective and less harmful to other water fauna. The present work aims at investigating the comparative effects of chlorine and Huwa-san as a new disinfectant on Biomphalaria alexandrina snails which act as bioindicators of toxicity, besides evaluating their miracicidal and cercaricidal activities. The results showed that Huwa-san was more toxic on tested snails than chlorine, where the LC50 values were 21 and 1368...
August 2, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28766153/molecular-cloing-and-bioinformatics-analysis-of-lactate-dehydrogenase-from-taenia-multiceps
#18
Cheng Guo, Yu Wang, Xing Huang, Ning Wang, Ming Yan, Ran He, Xiaobin Gu, Yue Xie, Weimin Lai, Bo Jing, Xuerong Peng, Guangyou Yang
Coenurus cerebralis, the larval stage (metacestode or coenurus) of Taenia multiceps, parasitizes sheep, goats, and other ruminants and causes coenurosis. In this study, we isolated and characterized complementary DNAs that encode lactate dehydrogenase A (Tm-LDHA) and B (Tm-LDHB) from the transcriptome of T. multiceps and expressed recombinant Tm-LDHB (rTm-LDHB) in Escherichia coli. Bioinformatic analysis showed that both Tm-LDH genes (LDHA and LDHB) contain a 996-bp open reading frame and encode a protein of 331 amino acids...
August 1, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28766152/haemosporidian-prevalence-and-parasitaemia-in-the-black-throated-sparrow-amphispiza-bilineata-in-central-mexican-dryland-habitats
#19
J G Ham-Dueñas, L Chapa-Vargas, C M Stracey, E Huber-Sannwald
To date it is not well-understood how seasonality and human-induced habitat change may affect haemosporidian prevalence and parasitaemia in bird hosts in dryland habitats. We compared haemosporidian prevalence and parasitaemia between habitat types, including Yucca-dominated scrublands (closed habitat) and creosotebush scrublands (open habitat), and between seasons, including non-breeding (dry) and breeding (wet) in the Black-throated sparrow (Amphispiza bilineata) at semi-arid scrublands of Central Mexico...
August 1, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28766151/anticoccidial-effects-of-artemisia-annua-ethanolic-extract-prevention-simultaneous-challenge-medication-and-treatment
#20
Ahmadreza Fatemi, Keramat Asasi, Seyed Mostafa Razavi
The effect of Artemisia annua ethanolic extract (AE) as a potential source of herbal anticoccidial activity was investigated on experimental coccidiosis in chicken. One hundred ninety-two one-day-old chicks were divided in to 8 groups (n = 24) including AE prevention group, AE-treated group, simultaneously challenged AE-medicated group, challenged-untreated group (positive control), unchallenged-untreated group (negative control), salinomycine prevention group, salinomycine-treated group, and simultaneously challenged salinomycine-medicated group, in a completely randomized design...
August 1, 2017: Parasitology Research
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