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Indian Journal of Medical Microbiology

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https://www.readbyqxmd.com/read/28303841/clinico-epidemiological-profile-of-dengue-cases-prevalent-in-lakhimpur-district-of-assam
#1
LETTER
Jitendra Sharma
No abstract text is available yet for this article.
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303840/prevalence-of-bi-component-pore-forming-toxin-genotypes-of-staphylococcus-aureus-causing-skin-and-soft-tissue-infections
#2
LETTER
Nagaraj Perumal, Betsy Sowndarya Dass, Stalin Mani, Padma Krishnan
No abstract text is available yet for this article.
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303839/emergence-of-st39-and-st656-extensively-drug-resistant-klebsiella-pneumoniae-isolates-in-wenzhou-china
#3
LETTER
Haiyang Liu, Jonathan Wilksch, Bin Li, Jia Du, Jianming Cao, Xiaoxiao Zhang, Tieli Zhou
No abstract text is available yet for this article.
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303838/introducing-rubella-vaccine-into-the-national-immunisation-schedule
#4
LETTER
Valsan Philip Verghese
No abstract text is available yet for this article.
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303837/prevalence-of-multiple-drug-resistant-plasmodium-falciparum-malaria-cases-in-northeast-india
#5
Jitendra Sharma, Siraj Ahmed Khan, Monika Soni, Prafulla Dutta
Two numbers of Plasmodium falciparum field isolates from Gossingpara, Runikhata area in Chirang district of Assam had shown multiple mutations in Pfcrt-dhfr-dhps gene (up to seven mutations: One mutation in Pfcrt gene, three mutations in Pfdhfr gene and three mutations in Pfdhps gene). Similarly, two cases in Bat camp, Miao area under Changlang district of Arunachal Pradesh had shown a total of eight mutations, of which one mutation in Pfcrt gene, three mutations in Pfdhfr gene, three mutations in Pfdhps gene and one mutation in PfATPase6 gene...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303836/carbapenem-nonsusceptibility-with-modified-oprd-in-clinical-isolates-of-pseudomonas-aeruginosa-from-india
#6
Debarati Choudhury, Anupam Das Talukdar, Manabendra Dutta Choudhury, Anand Prakash Maurya, Debadatta Dhar Chanda, Atanu Chakravorty, Amitabha Bhattacharjee
This study was undertaken to investigate OprD porin-mediated carbapenem nonsusceptibility in clinical isolates of Pseudomonas aeruginosa from a tertiary referral hospital of Northeast India. A total of 267 nonduplicate, consecutive clinical isolates of P. aeruginosa were obtained. Mutation and expression levels of OprD gene were determined in carbapenem-nonsusceptible carbapenemase-nonproducing isolates. Among 19 carbapenem-nonsusceptible carbapenemase-nonproducing isolates, 11 of them demonstrated variable band pattern while performing denaturing gradient gel electrophoresis with amplified products of OprD gene...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303835/trends-of-respiratory-syncytial-virus-sub-types-in-children-hospitalised-at-a-tertiary-care-centre-in-jaipur-during-2012-2014
#7
M Anjaneya Swamy, Bharti Malhotra, P V Janardhan Reddy, Jitendra Kumar Tiwari, Neeraj Kumar, Manohar Lal Gupta
Respiratory syncytial virus (RSV) causes high mortality and morbidity in infants. The study was planned to determine the trends of RSV sub-types in hospitalised children. Nasopharyngeal aspirate and throat swabs were collected from the hospitalised children up to 5 years of age. Viral nucleic acid was extracted using easyMAG automated extraction system, and real-time reverse transcription polymerase chain reaction was performed. Total positivity for RSV was found to be 25.40%, predominantly for RSV B (20.03%), followed by RSV A (2...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303834/trends-in-spoligotype-patterns-of-mycobacterium-tuberculosis-strains-in-central-india
#8
Prabha Desikan, Devendra S Chauhan, Nikita Panwalkar, Pragya Sharma, Priyanka Yadav
This study aims to understand trends in spoligotype patterns of Mycobacterium tuberculosis (MTB) in Central India. Elucidation of these trends may provide baseline information to understand the transmission dynamics of strains of MTB in the region. Spoligotyping was carried out on 340 MTB strains isolated from clinical samples received from 2007 to 2011. The prevalence of ST26/CAS1_Del, ST11/EAI3_IND, ST288/CAS2, ST25/CAS1_Del and Beijing lineages showed waxing and waning trends. ST26/CAS1_Del and ST11/EAI3_IND lineages were consistently present and were predominant...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303833/antimicrobial-susceptibility-of-leptospira-spp-isolated-from-environmental-human-and-animal-sources-in-malaysia
#9
Douadi Benacer, Siti Nursheena Mohd Zain, Peck Toung Ooi, Kwai Lin Thong
Leptospirosis is a zoonosis with worldwide distribution caused by pathogenic spirochetes of the genus Leptospira. The aim of this study was to evaluate the susceptibility of isolates obtained from different hosts. A total of 65 Leptospira isolates from humans (n = 1), zoonoses (rat, n = 60; dog, n = 1; swine, n = 1) and environment (n = 2) were tested against six antibiotics. All the isolates were resistant to trimethoprim and sulphamethoxazole and had high MIC toward chloramphenicol (MIC90: 6.25 μg/ml). All except one environment isolate were sensitive to ampicillin, doxycycline and penicillin G...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303832/coagulase-negative-staphylococci-emerging-pathogen-in-central-nervous-system-shunt-infection
#10
Preeti Lata Bhatia, Sunil Lilani, Ritesh Shirpurkar, Chhaya Chande, Shrikant Joshi, Abhay Chowdhary
Central nervous system (CNS) shunts are commonly used to treat patients with hydrocephalus. Its placement is associated with increased risk of infection. The study was intended to evaluate infection rate associated with CNS shunt surgeries and identify risk factors for shunt infection. The frequency and characterisation of aetiological agents along with their antibiotic resistance pattern were also studied. A prospective study of 86 patients who underwent 97 surgeries over a period of 18 months was conducted...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303831/strengths-and-limitations-of-various-screening-methods-for-carbapenem-resistant-enterobacteriaceae-including-new-method-recommended-by-clinical-and-laboratory-standards-institute-2017-a-tertiary-care-experience
#11
Agila Kumari Pragasam, Balaji Veeraraghavan, Yamuna Devi Bakthavatchalam, Radha Gopi, Raziya Fathima Aslam
Carbapenemase-mediated carbapenem resistance is a major concern across the world. Rapid detection of carbapenemase-producing organisms is of great importance in clinical settings. However, it is essential to have a test with good sensitivity and specificity. The aim of the study was to compare the performance of RAPIDEC® CARBA NP and modified carbapenem inactivation method (mCIM) recommended by Clinical and Laboratory Standards Institute guideline 2017. A total of ninety carbapenem resistant Escherichia coli and Klebsiella pneumoniae have been tested...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303830/multiplex-polymerase-chain-reaction-for-the-detection-of-high-risk-human-papillomavirus-types-in-formalin-fixed-paraffin-embedded-cervical-tissues
#12
Mini P Singh, Nalini Gupta, T Deepak, Archit Kumar, Radha Kanta Ratho
Detecting high-risk-human papillomavirus (HPV) types has become an integral part of the cervical cancer screening programmes. This study aimed to develop a multiplex polymerase chain reaction (PCR) for identification of HPV types 16 and 18 along with the beta globin gene in formalin-fixed and paraffin-embedded cervical biopsy specimens. A total of 59 samples from patients with cervical abnormalities were tested. HPV 16 positivity was 50% in cervical cancers and 52.9% in cervical intraepithelial neoplasia. Our multiplex PCR protocol can be used as a simple and cost-effective tool for high-risk-HPV detection in cervical cancer screening programmes...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303829/mutation-pattern-in-the-genome-of-neisseria-gonorrhoeae-and-its-association-with-multidrug-resistant-isolates-from-delhi-india
#13
Divya Sachdev, Indu Kumari, Manju Bala, Vineet Kumar, Daman Saluja
Mutations in PenA, PorB, MtrE and MtrR genes responsible for antimicrobial resistance were checked in 27 drug-resistant clinical isolates of Neisseria gonorrhoeae (NG). Phenotype PIB (88.88%) and mutation at G120 and A121 positions of porB were recurrent. N122K, a novel mutation, was observed in PorB in three resistant isolates. Substitution H105Y in MtrR was widespread (37% of clinical isolates). The presence of a novel mutation (L33V), along with G45D mutation in MtrR, was associated with less-resistant isolates, in contrast to isolates with G45D mutation alone...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303828/high-prevalence-of-cryptococcal-antigenaemia-amongst-asymptomatic-advanced-hiv-patients-in-pune-india
#14
Dileep Kadam, Ajay Chandanwale, Renu Bharadwaj, Neetal Nevrekar, Samir Joshi, Sandesh Patil, Nikhil Gupte, Shashi Sangle, Kavita Chopade, Vandana Kulkarni, Usha Balasubramanian, Nishi Suryavanshi, Divyashri Jain, Savita Kanade, Sujata Dharmashale, Anju Kagal, Amita Gupta, Vidya Mave
BACKGROUND: The World Health Organization recommends routine cryptococcal antigen (CrAg) screening in advanced AIDS patients initiating antiretroviral treatment (ART). India has yet to adopt this strategy as the burden of cryptococcal antigenaemia is unknown. METHODS: A prospective study was conducted in a large public sector ART centre and the inpatient wards of Sassoon Hospital, Pune, India. All consenting patients> 18 years of age with CD4 count <100 cells/mm3 were screened for CrAg by latex agglutination assay...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303827/characterisation-of-the-human-oral-microbiome-in-patients-with-coronary-artery-disease-using-next-generation-sequencing-of-16srrna-amplicons
#15
Thangam Menon, Sathya Narayanan Gopalakrishnan, Rayvathy Balasubramanian, Stalin Roy Justin
INTRODUCTION: Oral health is suspected to be linked to heart disease since species of bacteria that cause periodontitis and dental caries have been found in the atherosclerotic plaque in arteries in the heart. OBJECTIVES: The aim of this study was to characterize the oral microbiome in patients with coronary artery disease (CAD) and in a patient with dental caries (DC) without any clinical symptoms of CAD. METHODS: DNA was extracted from the oral swabs collected from the patients and sequencing was performed by next generation sequencing method using Illumina (MiSeq) platform...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303826/prevalence-of-pneumococcal-serotypes-in-adults-%C3%A2-50-years-of-age
#16
Chand Wattal, Neeraj Goel, S P Byotra
CONTEXT: Currently, majority of studies on antibiotic resistance and serotype prevalence in Streptococcus pneumoniae are in paediatric population and there is limited epidemiologic data pertaining to elderly Indian population. AIMS: This study was undertaken to assess antibiotic resistance pattern and the coverage of the polysaccharide vaccine 23 (PPV23) in the elderly population. SETTINGS AND DESIGN: This was a prospective 2-year pneumococcal surveillance study in patients with community-acquired pneumococcal infections of ≥50 years' age group on fifty isolates of S...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303825/evaluation-of-loop-mediated-isothermal-amplification-assay-for-rapid-diagnosis-of-acanthamoeba-keratitis
#17
Abhishek Mewara, Sumeeta Khurana, Shakila Yoonus, Kirti Megha, Parveen Tanwar, Amit Gupta, Rakesh Sehgal
BACKGROUND: The clinical features of Acanthamoeba keratitis (AK) are non-specific and closely resemble bacterial, viral and fungal keratitis. MATERIALS AND METHODS: We compared loop-mediated isothermal amplification (LAMP) with microscopy, non-nutrient agar (NNA) culture and polymerase chain reaction (PCR) in clinical suspects of AK. RESULTS: Of 52 clinical samples (42 AK suspects and 10 proven bacterial, viral or fungal keratitis), 3 were positive by direct microscopy (sensitivity 60%, confidence interval [CI]: 17%-92...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303824/matrix-assisted-laser-desorption-ionisation-time-of-flight-mass-spectrometry-rapid-detection-of-carbapenamase-activity-in-acinetobacter-baumannii-isolates
#18
Noha Abouseada, May Raouf, Eman El-Attar, Pacinte Moez
INTRODUCTION: Carbapenamase-producing Acinetobacter baumannii are an increasing threat in hospitals and Intensive Care Units. Accurate and rapid detection of carbapenamase producers has a great impact on patient improvement and aids in implementation of infection control measures. AIM: In this study, we describe the use of matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI TOF MS) to identify carbapenamase-producing A. baumannii isolates in up to 3 h...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303823/bacterial-and-fungal-biofilm-formation-on-contact-lenses-and-their-susceptibility-to-lens-care-solutions
#19
Siddharth Kackar, Ethel Suman, M Shashidhar Kotian
BACKGROUND: Microbial biofilm formation on contact lenses and lens storage cases may be a risk factor for contact lens-associated corneal infections. Various types of contact lens care solutions are used to reduce microbial growths on lenses. OBJECTIVES: The present study aimed at comparing the growths of biofilms on the different contact lenses and lens cases. The study also aimed at determining the effect of lens care solutions and bacteriophage on these biofilms...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303822/human-papillomavirus-and-chlamydia-trachomatis-infection-in-gyneco-obstetric-outpatients-from-a-mexican-hospital
#20
Laura Conde-Ferráez, Jorge Ramiro Carrillo Martíez, Guadalupe Ayora-Talavera, María Del Refugio González Losa
CONTEXT: Human papillomavirus (HPV) and Chlamydia trachomatis are the most frequent sexually transmitted infections, usually asymptomatic. Persistent infection with high-risk HPV types and other cofactors such as the concomitant infection with C. trachomatis can represent a higher risk to develop cervical lesions; therefore, screening with sensitive methods could aid to identify women at risk. AIMS: The aim is to determine the prevalence and concurrence of both infections, detected with in-house molecular methods, and to identify the risk factors associated to the infections in Mexican women...
January 2017: Indian Journal of Medical Microbiology
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