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Indian Journal of Medical Microbiology

Malini R Capoor, Kumar Tapash Bhowmik
No abstract text is available yet for this article.
October 2017: Indian Journal of Medical Microbiology
Ram Gopalakrishnan, Nandini Sethuraman, R Madhumitha, Kalpesh Sukhwani, Nitin Bansal, Indira Poojary, Arunaloke Chakrabarti
Cerebral phaeohyphomycosis is an infection caused by a number of dematiaceous fungi, characterised by the presence of melanised hyphae in the invaded tissue. Cladophialophora bantiana is the most common species affecting the humans, which has a predilection for causing the central nervous system infections resulting in high mortality. We hereby report a success story of two cases of brain abscess caused by C. bantiana who were treated with surgical source reduction and voriconazole therapy.
October 2017: Indian Journal of Medical Microbiology
Xiao-Hua Chi, Yao-Ming Xue, Quan-Shi Wang, Gui-Ping Li, Hong-Sheng Zhou, Yong-Shuai Qi
Penicillium marneffei infection in human immunodeficiency virus (HIV)-negative patients is addressed far less often. In this article, a small cohort of HIV-negative patients who disseminated P. marneffei infection was included. Sites of infection were found from blood culture, as subcutaneous nodules, or from lymph node biopsy. Fever, rash, swollen lymph nodes, anaemia and weight loss were common characteristics in most infected patients. The signs and symptoms are diverse and create challenges for accurate diagnosis...
October 2017: Indian Journal of Medical Microbiology
Harsimran Kaur, Balvinder Mohan, Vinaykumar Hallur, Atul Raj, Madhunarayana Basude, Ravimohan S Mavuduru, Neelam Taneja
Chryseobacterium species are rarely reported as aetiological agents of nosocomial urinary tract infection. Here, we evaluated the clinical significance of 19 isolates of Chryseobacterium species (15 Chryseobacterium indologenes and 4 Chryseobacterium gleum; identified by matrix-assisted laser desorption/ionisation-time of flight [MALDI-TOF]) obtained from urine or percutaneous nephrostomy drainage of 16 patients with urological complaints. The strains possessed drug resistance to multiple antibiotics. 14 isolates showed the presence of carbapenemases...
October 2017: Indian Journal of Medical Microbiology
Radhika Raman, Janani Sankar, Sulochana Putlibai, Vaidehi Raghavan
BACKGROUND: Pneumonia is a preventable cause of mortality in children. Streptococcus pneumoniae colonising the nasopharynx of healthy children can cause invasive diseases and the serotype distribution of colonisation isolates should be an indicator of invasive disease, antibiotic resistance profiles, and potential vaccine coverage. Identifying factors influencing nasopharyngeal colonisation, the serotypes and antimicrobial resistance pattern can improve rational preventive strategies...
October 2017: Indian Journal of Medical Microbiology
Vikas Khillan, Pratibha Kale, Viniyendra Pamecha, Neha Rathor, Shiv Kumar Sarin
Infections are the leading cause of morbidity and mortality in liver transplant (LT) recipients. We studied timeline, spectrum of infection, system involved, and antimicrobial resistance in 64 patients undergoing live donor LT with 6-month follow-up. Of 64 patients, 38 (59.5%) patients had 103 infectious episodes, 10 patients had single infectious episode and 28 patients had two or more infectious episodes. 96 (93.2%) were bacterial and Candida infections were in 7 (6.8%). Early phase had 30 (29.1%) episodes; intermediate phase 25 (24...
October 2017: Indian Journal of Medical Microbiology
Anisha Sarma, Lahari Saikia, Ratna Kanta Bhuyan, Ezaz Hussain
The present study aimed to detect the major virulence determinants of Helicobacter pylori, the gastric bacteria by polymerase chain reaction from genomic DNA of 314 gastric biopsies from dyspeptic patients in 2015-2016 through upper gastrointestinal endoscopy. In 153 cases out of 314, the high prevalence of oipA gene followed by cagA-vacA s1 m1 combined genotypes was found in mostly gastritis/duodenitis patients followed by the peptic ulcer and normal patients. Therefore, the clinical significance of the virulence markers of H...
October 2017: Indian Journal of Medical Microbiology
Kirti Megha, Amit Gupta, Rakesh Sehgal, Sumeeta Khurana
Acanthamoebae can be easily grown in bacterised cultures, but their growth in axenic media is tedious and many times unsuccessful. We thus experimented with some additives in the conventional axenic medium for growth of various isolates of Acanthamoeba. Addition of Torula yeast RNA was found to significantly enhance the growth of Acanthamoebae in the axenic culture medium.
October 2017: Indian Journal of Medical Microbiology
Isra Halim, Tushar Shaw, Chaitanya Tellapragada, K E Vandana, Chiranjay Mukhopadhyay
Melioidosis has recently gained importance as an emerging disease in India. Recurrent melioidosis has been reported from different parts of the world and can be due to relapse or reinfection. Distinction between relapse and reinfection is important for epidemiology, investigation and management. Here, we present the data regarding rate of recurrence and utility of multilocus sequence typing (MLST) in differentiating relapse form reinfection amongst melioidosis patients from a tertiary care hospital in South India...
October 2017: Indian Journal of Medical Microbiology
Reema Nath, Reeta Bora, Biswajyoti Borkakoty, Lahari Saikia, Pratap Parida
In this retrospective study from 2012 to 2015, 333 clinical isolates of yeasts were identified using Vitek 2 Compact System YST ID card (Biomerieux, France) and internal transcribed spacer (ITS) sequencing. Eighteen species were identified by ITS sequencing. Candida albicans was the most common species (46.5%), followed by Candida tropicalis (27%). The total species supported by Vitek System was 11 (61.11%). The sensitivity of the system in identifying these 11 species was 66.66%-100%; specificity 98.37%-100%; positive predictive value 70%-100%, negative predictive value 96...
October 2017: Indian Journal of Medical Microbiology
Balaji Veeraraghavan, Agila Kumari Pragasam, Abi Manesh, Priscilla Rupali, Ramya Iyadurai, Camilla Rodrigues, Sangeeta Joshi, Indranil Roy, Bhaskar Narayan Chaudhuri, D S Chitnis, Dhole Tapan
Aminoglycosides are important agents used for treating drug-resistant infections. The current dosing regimen of aminoglycosides does not achieve sufficient serum level concentration for the infected bacterial pathogen interpreted as susceptible based on laboratory testing. Minimum inhibitory concentration was determined for nearly 2000 isolates of Enterobacteriaceae and Pseudomonas aeruginosa by broth microdilution method. Results were interpreted based on CLSI and EUCAST interpretative criteria and the inconsistencies in the susceptibility profile were noted...
October 2017: Indian Journal of Medical Microbiology
B V Srinidhi, G John Fletcher, Jaiprasath Sachidanantham, Priscilla Rupali, Veena Vadhini Ramalingam, J P Demosthenes, O C Abraham, Susanne A Pulimood, Grace Rebekah, Rajesh Kannangai
PURPOSE: Type III interferon is well known to have diverse antiviral and immunomodulatory activities. Studies describing the association of interleukin (IL)-28 polymorphisms in treatment-experienced HIV participants are limited. This study was aimed to determine the association of IL-28B gene polymorphisms with immunological recovery in HIV patients on 6-9 months of antiretroviral therapy (ART). METHODS: Eighty treatment-naive HIV patients were recruited, of which 48 patients were followed up after 6-9 months of ART...
October 2017: Indian Journal of Medical Microbiology
Reena Rajkumari, John Melbin Jose, Kootallur Narayanan Brahmadathan
BACKGROUND: Reported literature on invasive group A streptococcal isolates in India is very scanty. This study was undertaken to determine the molecular heterogeneity of such isolates as seen in a tertiary care center. MATERIALS AND METHODS: Thirty two blood culture isolates and 18 from other sterile body fluids were characterized by emm gene sequencing and multilocus sequence typing. RESULTS: Forty two emm types were identified including 25 from 32 blood isolates and 17 from 18 other body fluid isolates...
October 2017: Indian Journal of Medical Microbiology
Shariq Ahmed, Indu Shukla, Nazish Fatima, Sumit K Varshney, Mohammad Shameem
BACKGROUND: Isoniazid (INH) and rifampicin (Rif) are the key first-line antituberculosis drugs, and resistance to these drugs i.e., multi-drug-resistant tuberculosis (MDR-TB), is likely to result in treatment failure and poor clinical outcomes. India has the highest burden of TB and MDR-TB in the world, disproportionately high even for India's population. The GenoType® MTBDRplus molecular method allows rapid detection of Rif and INH resistance. AIM: The present study was done to compare the performance of line probe assay test (GenoType® MTBDRplus) with solid culture method for an early diagnosis of MDR-TB...
October 2017: Indian Journal of Medical Microbiology
Satish Ramchandrra Patil, Kailash D Datkhile, M V Ghorpade, Supriya Satish Patil, Satish V Kakade
BACKGROUND AND OBJECTIVES: Hepatitis C is global health problem affecting a significant portion of the world's population. Available data in Western Maharashtra on seroprevalence, risk factors and genotype distribution are very limited. OBJECTIVES: The present study was carried out to estimate the seroprevalence, factors influencing transmission and distribution of genotype of hepatitis C virus (HCV) in a hospital-based population. MATERIALS AND METHODS: This was a cross-sectional, hospital-based study...
October 2017: Indian Journal of Medical Microbiology
Balvinder Mohan, Amber Prasad, Harsimran Kaur, Vinaykumar Hallur, Neha Gautam, Neelam Taneja
PURPOSE: Carbapenem-resistant Enterobacteriaceae (CRE) have emerged and disseminated widely causing a variety of infections. In India, the carriage of CRE in hospitalised patients has not been well-studied. Therefore, we conducted the present study to observe gut carriage rate of CRE in patients admitted to our tertiary care hospital. METHODS: A total of 232 faecal swabs collected from consecutive stool samples from admitted patients were inoculated on ChromID extended spectrum β-lactamase plates and members of Enterobacteriaceae family were subjected to antibiotic susceptibility as per the Clinical Laboratory Standards Institute guidelines...
October 2017: Indian Journal of Medical Microbiology
Kumar Saurabh, Shilpi Ranjan
PURPOSE: To describe the clinical and sociodemographic profile of fasciolopsiasis in children. MATERIALS AND METHODS: A chart review of 56 children presenting with the passage of adult Fasciolopsis buski per stool from February 2015 to January 2016 was done for their clinical profile and risk factors for acquiring fasciolopsiasis in the Paediatric Unit of a medical college of Northern India. RESULTS: The mean age of presentation was 8.2 years (2-14 years age group)...
October 2017: Indian Journal of Medical Microbiology
Swati Setty, Tanvee Wadikar, S S Suprith, Kishore Bhat, Srinath Thakur
BACKGROUND: A. actinomycetemcomitans is prevalent in periodontitis but is found in some periodontally healthy individuals as well. Certain serotypes of the organism have shown to determine severity of the disease. The distribution of serotype and genotype is affected by geographic and ethnic variation. Therefore, the present study was aimed to detect serotypes b & c of A. actinomycetemcomitans and the genotypes and find its correlation with periodontal status. MATERIALS AND METHODS: A total of 75 subjects (25 aggressive periodontitis, 25 chronic periodontitis and 25 periodontally healthy) in age range of 14-55 yrs were included...
October 2017: Indian Journal of Medical Microbiology
Suphi Bayraktar, Nizami Duran, Gülay Gülbol Duran, Naciye Eryilmaz, Hayat Aslan, Cansu Önlen, Burçin Özer
AIM: We aimed to identify the distribution of species in candidal strains isolated from clinical samples and restriction fragment length polymorphism (RFLP) method based on Msp I and Bln I restrictive enzyme cuts of polymerase chain reaction (PCR) products after the amplification of ITS1 and ITS2 regions of rDNA genotypically. MATERIALS AND METHODS: One hundred and fifty candidal strains isolated from various clinical samples were studies/ included. Phenotypic species assessment was performed using automated VITEK-2 system and kit used with the biochemical tests...
October 2017: Indian Journal of Medical Microbiology
Jitendra Kumar Tiwari, Bharti Malhotra, Aradhana Chauhan, Hemant Malhotra, Pratibha Sharma, Farah Deeba, Khushbu Trivedi, Anjenya M Swamy
CONTEXT: Acute encephalitis syndrome (AES) is a serious public health problem, caused mainly by viruses. However, the profile of viruses causing AES in Rajasthan is not well characterised. AIMS: The present study was undertaken to identify the viruses causing AES and develop diagnostic algorithm so as to help in improved diagnosis, treatment, prevention and control. SETTINGS AND DESIGN: The present study is a hospital-based descriptive, observational study...
October 2017: Indian Journal of Medical Microbiology
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