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Indian Journal of Medical Microbiology

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https://www.readbyqxmd.com/read/30084430/the-continuing-scourge-of-congenital-syphilis-in-the-face-of-declining-seropositivity-a-challenge-to-control-efforts
#1
LETTER
Srujana Mohanty, Tapas Som, Ashok Kumar Praharaj
No abstract text is available yet for this article.
April 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/30084429/high-prevalence-of-varicella-seronegativity-in-nurses-at-a-tertiary-care-private-hospital-in-mumbai-india
#2
LETTER
Sweta Shah, Tanu Singhal, Reshma Naik, Pooja Thakkar
No abstract text is available yet for this article.
April 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/30084428/oral-administration-of-klebsiella-pneumoniae-specific-bacteriophage-eradicates-the-bacteria-in-albino-mice
#3
LETTER
Ashutosh Chaturvedi, Gopal Nath
No abstract text is available yet for this article.
April 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/30084427/clinical-spectrum-susceptibility-profile-treatment-and-outcome-of-culture-confirmed-brucellosis-from-south-india
#4
Selvin Sundar Raj Mani, Karthik Gunasekaran, Ramya Iyyadurai, John Antony Jude Prakash, Balaji Veeraraghavan, Ajay Kumar Mishra, Kirti Sabnis, Punitha John Victor, Sherry Martin, Vignesh Kumar Chandiraseharan, Samuel George Hansdak, George M Varghese
Brucellosis, a common zoonosis, is under reported in India despite its endemicity and increased exposure to livestock among the population. This study was conducted to determine the clinical manifestations, antibiotic susceptibility pattern, treatment and outcome of culture confirmed brucellosis. Adult patients with culture confirmed brucellosis who presented to a large teaching hospital in South India between 2009 and 2015 were included. A diagnosis of brucellosis was confirmed on automated culture. Clinical profile, laboratory parameters, drug susceptibility, treatment and outcome were documented by reviewing the medical records...
April 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/30084426/alarming-resistance-of-neisseria-gonorrhoeae-in-a-tertiary-care-hospital-of-north-india
#5
Mohit Sharma, Meher Rizvi, Richa Gupta, Mohd Azam, Haris M Khan, Anjum Parvez, Indu Shukla
Background: This study attempted to elucidate the spectrum of sexually transmitted infections in a tertiary care centre in North India and to assess the antimicrobial resistance in Neisseria gonorrhoeae. Materials and Methods: Antimicrobial resistance pattern of N. gonorrhoeae was determined by the standard techniques. Genotypic detection of gyrA, parC and blaTEM genes was also carried out. The results of gyrA gene by polymerase chain reaction were confirmed by DNA sequencing...
April 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/30084425/twenty-one-episodes-of-peritonitis-in-a-continuous-ambulatory-peritoneal-dialysis-patient-what-is-the-root-cause
#6
Victorine B Nzana, Anusha Rohit, Deepu George, Madhusudan Vijayan, Milly Mathew, Sundar Sankaran, Palaniappan Nagarajan, Georgi Abraham
A 51-year-old female, with non-alcoholic liver cirrhosis, portal hypertension, type 2 diabetes mellitus, autosomal dominant polycystic kidney disease with a clipped cerebral aneurysm and chronic kidney disease stage 5 was on continuous ambulatory peritoneal dialysis (CAPD) for 6.5 years elsewhere. She came for opinion on continuation of CAPD as she had 21 episodes of peritonitis in 76 months. Her blood pressure was 80/50 mmHg. She was on haemodialysis with a temporary central access for 2 weeks. She had no abdominal tenderness, and exit site looked normal...
April 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/30084424/intraventricular-haemorrhage-as-the-first-manifestation-of-congenital-cytomegalovirus-infection
#7
Malgorzata Sobolewska-Pilarczyk, Katarzyna Pawlak-Osinska, Sylwia Drewa, Beata Smok, MaƂgorzata Pawlowska
Congenital Cytomegalovirus infection (CCMV) is the most common intrauterine infection. Early diagnosis of CCMV is hindered by three factors: There is no screening programme for CMV infection in pregnant women; a high percentage of infections in neonates are asymptomatic; the clinical signs of CCMV infection are uncharacteristic. The aim of this article is to analyse the clinical picture and course of CCMV treatment in a 3-week-old newborn, analyse adverse events in 14-week-long antiviral therapy and also assess intraventricular bleeding as an early indicator for the diagnosis of CCMV...
April 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/30084423/identification-of-prevalent-dengue-serotypes-by-reverse-transcriptase-polymerase-chain-reaction-and-correlation-with-severity-of-dengue-as-per-the-recent-world-health-organization-classification-2009
#8
Tanmay K Mehta, Parul D Shah
Context: A definite link between distinct dengue serotypes and severe clinical manifestations has not been established yet. The WHO classification (2009) of dengue is more competent in diagnosing severe cases compared to traditional (1997) classification. Aims: This study aimed to identify prevalent dengue serotypes and to correlate the severity of dengue with the dengue virus (DENV) serotypes in target population as per the recent WHO classification (2009). Settings and Design: A retrospective comparative observational study was conducted from 1st January 2015 to 31st December 2015...
April 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/30084422/antibiotic-resistance-in-helicobacter-pylori-a-mutational-analysis-from-a-tertiary-care-hospital-in-kashmir-india
#9
Fayaz Ahmad Wani, Gulnaz Bashir, Mushtaq Ahmad Khan, Showkat Ali Zargar, Zubaida Rasool, Qurteeba Qadri
Background: Helicobacter pylori infection is recognised as type 1 carcinogen by the International Agency of Research on Cancer. Previous studies in our hospital have revealed high prevalence of H. pylori in our population with a high recurrence rate after completion of treatment. This prompted us to undertake this study. Aim: This study aimed to determine common gene mutations leading to resistance to clarithromycin, metronidazole, tetracycline and quinolones in H...
April 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/30084421/molecular-investigation-of-staphylococcal-cassette-chromosome-mec-types-and-genotypic-relations-of-methicillin-resistant-staphylococci-isolated-from-before-and-after-hospital-exposed-students
#10
Bora Ekinci, Tuba Gokdogan Edgunlu, Gulcin Bayramoglu, Gulsen Ulucam Atay
Background: Reservoir of methicillin resistance genes called staphylococcal cassette chromosome mec (SCCmec), plasmids and genomic characterisations of isolates have been widely investigated in epidemiologic research. However, the extent to which these organisms are transported by patients or hospital staff is not entirely clear. Aim: This study aims to investigate the molecular relatedness and plasmid profiles of MR staphylococci isolated from nursing students before and after hospital training, to find out the possible source...
April 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/30084420/assessment-of-microbiological-status-after-successful-completion-of-intermittent-revised-national-tuberculosis-control-programme-directly-observed-treatment-short-course-regimen-for-microbiologically-confirmed-pulmonary-tuberculosis-cases-while-new-daily-regimen
#11
Roumi Ghosh, Sudipta Roy, Md Khalid Rashid
Purpose: The Revised National Tuberculosis Control Programme (RNTCP) is now introducing daily fixed-dose regimen instead of Directly Observed Treatment, Short Course (DOTS) regimen for treatment of drug-sensitive tuberculosis (TB) in India. It would be beneficial to understand the drawbacks, barriers and advantages of the existing system for better implementation of new policy. Our study was aimed to evaluate the current microbiological status of new microbiologically confirmed pulmonary TB patients who have successfully completed intermittent DOTS regimen within last 2 years and also to find the economic barriers faced by beneficiaries to avail DOTS treatment...
April 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/30084419/intestinal-cryptosporidiosis-in-renal-transplant-recipients-prevalence-species-detection-and-comparative-evaluation-of-ssu-rrna-and-cryptosporidium-oocyst-wall-protein-genes
#12
Ujjala Ghoshal, Prabhat Ranjan, Asmita Dey, Uday Chand Ghoshal
Context: Cryptosporidiosis is intestinal opportunistic infection commonly occurring in immunocompromised patients including renal transplant (RT) recipients receiving continuous immunosuppressive therapy. Knowledge about species of Cryptosporidium-infecting RT recipients is necessary to know about mode of its transmission (anthroponotic or zoonotic). Various genes such as small subunit rRNA (SSU rRNA) and Cryptosporidium oocyst wall protein (COWP) genes may help in species identification though their sensitivity and specificity are highly variable...
April 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/30084418/performance-of-an-in-house-real-time-pcr-assay-for-detecting-cytomegalovirus-infection-among-transplant-patients-from-a-tertiary-care-centre
#13
Santhosh Kumar Duraisamy, Shoba Mammen, Sasi Kumar Reddy Lakshminarayan, Susan Verghese, Mahesh Moorthy, Biju George, Rajesh Kannangai, Santosh Varghese, Alok Srivastava, Asha Mary Abraham
Background: Quantitative Cytomegalovirus (CMV) polymerase chain reactions are increasingly being used for monitoring CMV DNAemia in haematopoietic stem cell transplants and solid organ transplants. Objective: In this study, a commercial CMV viral load assay was compared with an in-house viral load assay. Materials and Methods: A total of 176 whole-blood samples were tested for CMV DNAemia using both assays. Results: Our evaluation showed a difference of 1 log10 copies/ml between the two assay systems in determining CMV viral loads in the clinical samples...
April 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/30084417/co-circulation-of-four-dengue-serotypes-at-south-eastern-andhra-pradesh-india-a-prospective-study
#14
Rishi Gowtham Racherla, Madhavi Latha Pamireddy, Alladi Mohan, Nagaraja Mudhigeti, Padmalatha Anjaneyulu Mahalakshmi, Umapathi Nallapireddy, Usha Kalawat
Background: Dengue is one of the most important mosquito-borne viral diseases in the world. The emergence and spread of four dengue viruses (DENVs) (serotypes) represent a global pandemic. The four distinct serotypes are, namely, DENV-1, DENV-2, DENV-3 and DENV-4. Very few dengue serotyping studies have been reported from Andhra Pradesh. In this context, the present study focuses on the circulating serotypes of dengue in South-Eastern Andhra Pradesh. Methodology: Study was done at Sri Venkateswara Institute of Medical Sciences, a teaching hospital in Tirupati, Andhra Pradesh...
April 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/30084416/detection-of-anti-hepatitis-c-virus-and-hepatitis-c-virus-rna-in-dried-blood-spot-specimens-using-whatman-no-1-filter-paper
#15
Ritapa Ghosh, Naba Kumar Hazarika
Purpose: Dried blood spot (DBS) specimen simplifies blood collection, processing, storage and shipment and may reduce the cost of testing for hepatitis C virus (HCV) infection. We wanted to see if DBS using a cheap filter paper is reliable alternative to serum for detection of anti-HCV and HCV RNA. Materials and Methods: At a tertiary care hospital in Northeast India, we collected 91 paired DBS and serum specimens from patients at risk of HCV infection from July 2014 to June 2015...
April 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/30084415/the-effectiveness-of-sofosbuvir-and-daclatasvir-in-the-treatment-of-hepatitis-c-in-thalassaemia-major-patients-and-their-effect-on-haematological-factors
#16
Farhad Zamani, Hossein Ajdarkosh, Fahimeh Safarnezhad-Tameshkel, Azita Azarkeivan, Hossein Keyvani, Farshad Naserifar, Jamshid Vafaeimanesh
Context: Patients with thalassaemia are at risk of infections such as hepatitis C virus (HCV) due to their repeated blood transfusions; meanwhile, the treatment of thalassaemia patients who had developed HCV infection is a controversial issue. Aims: Although the effectiveness of direct-acting antivirals on HCV infection has been confirmed, their side-effects as well as effects on haematological factors due to the resultant need for blood transfusion remain to be further understood...
April 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/30084414/lamivudine-plus-tenofovir-versus-lamivudine-plus-adefovir-for-the-treatment-of-hepatitis-b-virus-in-hiv-coinfected-patients-starting-antiretroviral-therapy
#17
Jayeeta Sarkar, Debraj Saha, Bhaswati Bandyopadhyay, Bibhuti Saha, Runu Chakravarty, Subhasish Kamal Guha
Background: Combination of tenofovir disoproxil fumarate (TDF), lamivudine (3TC) and efavirenz (EFV) is preferred in the treatment of HIV/hepatitis B virus (HBV) coinfection. We postulated that a HBV active nucleoside reverse transcriptase (RT) inhibitor/nucleotide RT inhibitor backbone of adefovir dipivoxil (ADV) +3TC would be as effective as TDF +3TC for the Indian population. Objective: ADV + 3TC could be an alternative option for these HIV/HBV coinfected individuals, preserving the dually active TDF + 3TC as second-line nucleoside backbone following failure of the first-line ART...
April 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/30084413/rapid-detection-of-ethambutol-resistant-mycobacterium-tuberculosis-in-clinical-specimens-by-real-time-polymerase-chain-reaction-hybridisation-probe-method
#18
D S Chauhan, R Sharma, D Parashar, R Das, P Sharma, A V Singh, P K Singh, K Katoch, V M Katoch
Background: Early diagnosis of drug resistance (DR) to ethambutol (EMB) in tuberculosis (TB) remains a challenge. Simple and reliable method (s) are needed for rapid detection of DR Mycobacterium tuberculosis (MTB) in clinical specimens. Objectives: The aim of this study was to design fluorescence resonance energy transfer hybridisation probe-based real-time polymerase chain reaction (PCR) method for the early detection of EMB-resistant MTB direct from clinical sputa...
April 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/30084412/polymorphisms-in-cytotoxic-t-lymphocyte-associated-antigen-4-gene-does-not-affect-scytotoxic-t-lymphocyte-associated-antigen-4-levels-in-human-papillomavirus-infected-women-with-or-without-cervical-cancer
#19
Priyanka Wagh, Priyanka Kulkarni, Shilpa Kerkar, Himangi Warke, Hemangi Chaudhari, Kedar Deodhar, Bharat Rekhi, Hemant Tongaonkar, Jayanti Mania-Pramanik
Background: Cervical cancer (CaCx) is the second most common cancer in Indian women. Cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) + 49 AA polymorphism is known to be associated with CaCx. Current attempt is to use immunotherapy for the treatment of metastatic melanoma and metastatic castration-resistant prostate cancer, i.e., blocking of CTLA-4 using a fully human monoclonal CTLA-4 antibody to disrupt its inhibitory signal. This allows the CTLs to destroy the cancer cells. There is no information available on the soluble level of CTLA-4 on which the immunotherapy is targeted...
April 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/30084411/prothrombotic-state-in-hiv-a-study-on-protein-c-protein-s-homocysteine-and-correlation-with-cd4-counts
#20
Swati Khare, Rashmi Kushwaha, Ashutosh Kumar, Vimala Venkatesh, Himanshu D Reddy, Mili Jain, Mohd Yusuf, Uma Shankar Singh
Introduction: Human immunodeficiency virus (HIV) may result in variable haematological manifestations. Thrombotic events are more common among HIV-infected persons than the general population, possibly due to the increased inflammatory/hypercoagulable state and presence of concurrent comorbidities. Aims and Objectives: (1) Screen for coagulation abnormalities in HIV-infected patients. (2) Detect certain prothrombotic factors such as deficiency of protein C and protein S and elevation of homocysteine as possible precursors of coagulation defects in HIV patients...
April 2018: Indian Journal of Medical Microbiology
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