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Indian Journal of Medical Microbiology

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https://www.readbyqxmd.com/read/27934850/erratum-endemic-indian-clones-of-klebsiella-pneumoniae-harbouring-new-delhi-metallo-beta-lactamase-1-on-a-hybrid-plasmid-replicon-type-a-case-of-changing-new-delhi-metallo-beta-lactamase-plasmid-landscapes-in-india
#1
(no author information available yet)
[This corrects the article DOI: 10.4103/0255-0857.188314.].
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27934849/polymyxin-nordmann-poirel-test-for-rapid-detection-of-polymyxin-resistance-in-enterobacteriaceae-indian-experience
#2
LETTER
Yamuna Devi Bakthavatchalam, Balaji Veeraraghavan, Purva Mathur, Swathi Purighalla, Vijay Samuel Richard
No abstract text is available yet for this article.
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27934848/medical-council-of-india-circular-on-research-publications-flaring-up-the-fire
#3
LETTER
D Juyal, V Thawani, S Thaledi, B Dhawan
No abstract text is available yet for this article.
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27934847/stamp-s-modified-ziehl-neelsen-staining-for-brucella-beware-of-the-first-impressions
#4
LETTER
K Tilak, V K Eshwara, C Tellapragada, C Mukhopadhyay
No abstract text is available yet for this article.
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27934846/gonococcal-opa-gene-as-a-diagnostic-target-for-nucleic-acid-amplification-tests-in-indian-population
#5
LETTER
R Verma, N Mahajan, S Sood
No abstract text is available yet for this article.
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27934845/in-vitro-susceptibility-of-carbapenem-resistant-enterobacteriaceae-to-colistin-a-hope-at-present
#6
LETTER
S Mohanty, R Gaind
No abstract text is available yet for this article.
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27934844/severe-unresolving-plasmodium-falciparum-malaria-following-artemisinin-combination-therapy-emergence-of-drug-resistance-in-saudi-arabia
#7
I A Al-Zaydani, A Al-Hakami, A Kumar, S A Abdalla, M Otaif, Rma Thiqa, H Ahmed, K Alnahili
A 5-year-old female child presented with fever of 1-week duration after visiting a malaria endemic zone without antimalarial prophylaxis. The patient presented with respiratory distress, decreased level of consciousness and high-grade fever. An elevated parasitaemia reaching 800,000/μl was observed. Antimalarial therapy was initiated with artesunate being administered intravenous (IV) along with IV clindamycin. Contrary to the expectations, there was no resolution of fever. Following a week of unresolved fever, the drug therapy was revised and altered to IV quinine dihydrochloride and IV clindamycin...
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27934843/successful-treatment-of-primary-cerebral-mucormycosis-role-of-microbiologist
#8
K K Benachinmardi, P Rajalakshmi, H B Veenakumari, R D Bharath, V Vikas, A Mahadevan, S Nagarathna
Fungal brain abscess is rare with a rapidly progressive disease with fulminant course and invariably fatal outcome, unless diagnosed early and treated rapidly. We report a 56-year-old woman diagnosed to have fungal abscess who recovered completely following amphotericin B treatment. She presented with weakness of the right hand, deviation of mouth to left and aphasia for 2 days. Computed tomography of the brain revealed a left frontal capsuloganglionic hypodense lesion. Stereotactic biopsy was performed, and microbiological confirmation of non-septate fungal hyphae from pus from aspirate within 2 h helped initiate timely and appropriate treatment leading to cure...
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27934842/infections-related-to-granulicatella-adiacens-report-of-two-cases-and-review-of-literature
#9
REVIEW
S Macin, A Ç İnkaya, Ö Tuncer, S Ünal, Y Akyön
Infections due to nutritionally variant streptococci are diagnosed rarely due to difficulties encountered during identification and isolation. Mortality rate in these infections is high therefore appropriate supplemented media and reliable detection systems should be implemented to isolate these fastidious organisms. Here, we describe two cases of Granulicatella adiacens infections. All microbiologic identifications were made with MALDI-TOF Vitek MS (BioMerieux, France), and the results confirmed by 16S ribotyping...
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27934841/aeromonas-hydrophila-meningitis-and-fulminant-sepsis-in-preterm-newborn-a-case-report-and-review-of-literature
#10
REVIEW
A Kali, R Kalaivani, Pmv Charles, K S Seetha
Neonatal meningitis is a lethal infection occurring in the 1st month of life. The risk of developing permanent neurological sequels is high among the neonates who survive. Bacterial pathogens are commonly associated with this condition. Aeromonas is a Gram-negative bacteria of aquatic habitat. Although isolation of Aeromonas species from neonates with blood stream infection is infrequently reported, neonatal meningitis caused by Aeromonas is exceedingly rare. We present a case of fulminant sepsis and meningitis caused by Aeromonas hydrophila in a preterm newborn male...
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27934840/application-of-real-time-quantitative-polymerase-chain-reaction-assay-to-detect-legionella-pneumophila-in-patients-of-community-acquired-pneumonia-in-a-tertiary-care-hospital
#11
A Angrup, R Chaudhry, S Sharma, A Valavane, K Passi, K Padmaja, S Javed, A B Dey, B Dhawan, S K Kabra
Legionella pneumophila is one of the important pathogen responsible for community -acquired pneumonia attributing for 1-5% of cases. Since early and accurate therapy reduces mortality, rapid and reliable diagnostic methods are needed. A total of 134 samples of blood, urine and respiratory tract fluids were collected. Blood was tested for IgG, IgM and IgA antibodies using commercially available kits. A total of 8 (6%) samples were found to be positive for L. pneumophila by quantitative reverse transcription polymerase chain reaction (qRT-PCR), compared to conventional PCR where 6 (4...
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27934839/internalisation-of-hepatitis-c-virus-core-protein-by-human-conjunctival-fibroblasts
#12
A R Rajalakshmy, J Malathi, H N Madhavan, S Bhaskar, G K Iyer
Recent studies indicate that hepatitis C virus (HCV) proteins can mediate innate immune response and inflammation in conjunctival fibroblasts which contributes to the pathology of dry eye condition associated with chronic HCV infection. The present study investigates the phagocytic potential of human conjunctival fibroblasts (HCFj) for HCV core protein. HCFj cells were incubated with HCV core antigen for different periods of time, and fluorescent micrographs were taken to observe protein internalisation. HCFj cells were capable of internalising HCV core antigen within 1 h; this gives an insight into another molecular mechanism which may contribute towards HCV-associated conjunctival inflammation...
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27934838/a-high-yield-dna-extraction-method-for-medically-important-candida-species-a-comparison-of-manual-versus-qiacube-based-automated-system
#13
COMPARATIVE STUDY
P Das, P Pandey, A Harishankar, M Chandy, S Bhattacharya
The prognosis of infected individuals with candidemia depends on rapid and precise diagnosis which enables optimising treatment. Three fungal DNA extraction protocols have been compared in this study for medically important Candida species. The quality and quantity of the DNA extracted by physical, chemical and automated protocols was compared using NanoDrop ND-2000 spectrophotometer. It was found that the yield and purity (260/230) ratio of extracted DNA was significantly high in the physical treatment-based protocol as compared to chemical based or automated protocol...
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27934837/high-fungal-spore-burden-with-predominance-of-aspergillus-in-hospital-air-of-a-tertiary-care-hospital-in-chandigarh
#14
S M Rudramurthy, G Singh, V Hallur, S Verma, A Chakrabarti
The prevalence of fungal spores in the hospital air is essential to understand the hospital-acquired fungal infections. Air conditioners (ACs) used in hospitals may either reduce spores in air or be colonised by fungi and aid in its dissemination. The present study was conducted to assess the fungal spore burden in AC and non-AC areas. We found a high fungal spore count in air irrespective of whether the area was AC or non-AC. The most predominant species isolated were Aspergillus flavus and Aspergillus fumigatus...
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27934836/improved-detection-of-shigella-using-escherichia-coli-medium-enrichment-polymerase-chain-reaction-from-stool-samples
#15
P K Gupta, S B Appannanavar, B Mohan, N Taneja
Laboratory diagnosis of shigellosis using conventional culture technique is limited by lower sensitivity and higher turnaround time. Here, we have evaluated the role of polymerase chain reaction from stool samples after enrichment in Escherichia coli medium for detection of Shigellae. The technique not only increased the sensitivity but also decreased the turnaround time.
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27934835/six-year-susceptibility-trends-and-effect-of-revised-clinical-laboratory-standards-institute-breakpoints-on-ciprofloxacin-susceptibility-reporting-in-typhoidal-salmonellae-in-a-tertiary-care-paediatric-hospital-in-northern-india
#16
R Saksena, C Nayyar, V Manchanda
The antimicrobial trends over 6 years were studied, and the effect of revised Clinical Laboratory Standards Institute (CLSI) breakpoints (2012) for ciprofloxacin susceptibility reporting in typhoidal Salmonellae was determined. A total of 874 (95.4%) isolates were nalidixic acid-resistant (NAR). Using the CLSI 2011 guidelines (M100-S21), 585 (66.9%) isolates were ciprofloxacin susceptible. The susceptibility reduced to 11 (1.25%) isolates when interpreted using 2012 guidelines (M100-S22). Among the forty nalidixic acid susceptible (NAS) Salmonellae, susceptibility to ciprofloxacin decreased from 37 isolates (M100-S21) to 12 isolates (M100-S22)...
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27934834/pulmonary-mycobacterium-kansasii-disease-in-immunocompetent-host-treatment-outcomes-with-short-course-chemotherapy
#17
C Padmapriyadarsini, D Nair, N S Gomathi, B Velayudham
Mycobacterium kansasii, most virulent of all atypical mycobacteria, causes pulmonary disease identical to the disease caused by Mycobacterium tuberculosis. Early identification of the species and prompt initiation of treatment for M. kansasii is necessary to prevent morbidity and mortality due to this disease. This case series highlights the similarity in the clinical presentation of both M. tuberculosis and M. kansasii and response to direct observation of short-course chemotherapy with rifampicin, in the management of pulmonary M...
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27934833/can-minocycline-be-a-carbapenem-sparing-antibiotic-current-evidence
#18
B Veeraraghavan, C Shankar, S Vijayakumar
With the increasing incidence of multidrug-resistant organisms, there is a need for newer antibiotics. However, due to the lack of new antimicrobial agents, it is necessary to re-evaluate the older agents like minocycline which is a second-line antimicrobial agent. In this study, minocycline susceptibility testing was performed for 693 Escherichia coli, 316 Klebsiella spp. and 89 Acinetobacter spp. Among extended spectrum beta-lactamase producing E. coli and Klebsiella spp. percentage susceptibility to minocycline were 76 and 85, respectively...
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27934832/in-vitro-sensitivity-pattern-of-chloroquine-and-artemisinin-in-plasmodium-falciparum
#19
Supriya Sharma, Kamlesh Kaitholia, Neelima Mishra, Bina Srivastava, C R Pillai, Neena Valecha, Anupkumar R Anvikar
Artemisinin (ART) and its derivatives form the mainstay of antimalarial therapy. Emergence of resistance to them poses a potential threat to future malaria control and elimination on a global level. It is important to know the mechanism of action of drug and development of drug resistance. We put forwards probable correlation between the mode of action of chloroquine (CQ) and ART. Modified trophozoite maturation inhibition assay, WHO Mark III assay and molecular marker study for CQ resistance at K76T codon in Plasmodium falciparum CQ-resistant transporter gene were carried out on cultured P...
October 2016: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27934831/synergism-between-fluconazole-and-methylene-blue-photodynamic-therapy-against-fluconazole-resistant-candida-strains
#20
J P Lyon, C R Carvalho, R R Rezende, C J Lima, F V Santos, L M Moreira
Photodynamic therapy (PDT) has been proved to be effective against fungi and it may be employed as a coadjutant to conventional antifungal agents, leading to a more effective microbial control minimising side effects. This work evaluates the combined effect of PDT and fluconazole against resistant Candida albicans, Candida glabrata and Candida krusei. The yeasts were submitted to methylene blue-PDT (MB-PDT) in sub-inhibitory concentrations. In the present work, MB-PDT combined with fluconazole was more efficient in the inhibition of the C...
October 2016: Indian Journal of Medical Microbiology
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