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Lasers in Medical Science

Kun-Tsung Denzel Lee, Min-Hsuan Chiang, Ping-Ho Chen, Mei-Ling Ho, Hong-Zin Lee, Huey-Er Lee, Yan-Hsiung Wang
Hyperglycemia-induced inflammation can greatly increase the risk of periodontal disease in people with diabetes. Low-level laser irradiation (LLLI) has been used for wound healing and anti-inflammation in many cases, and LLLI is known to inhibit the lipopolysaccharide (LPS)-stimulated inflammatory response. However, the therapeutic effect of LLLI in diabetes patients with periodontitis remains unknown. In this study, we cultured human gingival fibroblasts (HGFs) in high-glucose medium (35 mM) to mimic a hyperglycemic environment, and then measured the anti-inflammatory effect of LLLI by assessing the expression of pro-inflammatory genes including tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, and IL-8 by quantitative real-time polymerase chain reaction...
November 19, 2018: Lasers in Medical Science
Liying Wang, Fan Wu, Chen Liu, Yang Song, Jiawen Guo, Yanwei Yang, Yinong Qiu
The aim of this in vitro study was to evaluate the effects of low-level laser therapy (LLLT) at different energy intensities on proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) under healthy and inflammatory microenvironments. Human BMSCs and BMSCs from inflammatory conditions (i-BMSCs, BMSCs treated with tumor necrosis factor α; TNF-α) were subject to LLLT (Nd:YAG;1064 nm) at different intensities. We designed one control group (without irradiation) and four testing groups (irradiation at 2, 4, 8, and 16 J/cm2 ) for both BMSCs and i-BMSCs...
November 19, 2018: Lasers in Medical Science
Vanessa Batista da Costa Santos, Julio Cesar Molina Correa, Priscila Chierotti, Giovana Stipp Ballarin, Dari de Oliveira Toginho Filho, Fábio Yuzo Nakamura, Solange de Paula Ramos
Cryotherapy and phototherapy have been suggested as recovery methods due to their anti-inflammatory effects. They may also induce mitochondrial biogenesis, thus favoring endurance training adaptation. The aim of this study was to evaluate the anti-inflammatory and ergogenic effects of phototherapy or cold water immersion (CWI) applied daily after exercise in rats. Thirty-five rats were divided into five groups: control (CO), non-exercised (CE), passive recovery (PR), cold water immersion (CWI), and LED therapy (LED)...
November 19, 2018: Lasers in Medical Science
Shigeki Uchinuma, Yasushi Shimada, Khairul Matin, Keiichi Hosaka, Masahiro Yoshiyama, Yasunori Sumi, Junji Tagami
The aim of this study was to evaluate the bactericidal effect of ultraviolet (UV) light at 265-nm (UVC) and 310-nm (UVB) wavelengths from a newly developed UV light-emitting diode (LED) device against cariogenic bacteria in vitro. Suspensions of Streptococcus mutans (S. mutans) and Streptococcus sobrinus were directly irradiated by UVB or UVC for 2.5 min or 5 min. Numbers of colonies were counted and calculated as colony forming units (CFU) per milliliter. Fluorescence microscopy (FM) and optical density measurements at 490 nm (OD490 ) were also taken after irradiation...
November 17, 2018: Lasers in Medical Science
Zhi Yang, Huiyi Xiang, Xiaoxia Duan, Jianmeng Liu, Xiaolin He, Yunting He, Shaoyu Hu, Li He
The 1064-nm Q-switched neodymium-doped yttrium aluminum garnet (Nd:YAG) laser is widely used in clinical practice. However, the effects of 1064-nm Q-switched Nd:YAG laser on skin collagen generation have not been fully elucidated. The objectives of the present study were to investigate whether the 1064-nm Q-switched Nd:YAG laser can be used for non-ablative rejuvenation and to explore the possible mechanism underlying the effects. Six-week-old SKH-1 hairless mice were irradiated by the 1064-nm Nd:YAG laser at fluences of 0, 0...
November 17, 2018: Lasers in Medical Science
Pinar Serdar-Eymirli, M D Turgut, A Dolgun, A R Yazici
The aim of this in vitro study was to determine the effect of different remineralization agents and laser on caries resistance of primary enamel. In the study, 150 sound primary molars were used. The initial microhardness values were measured and the teeth were randomly assigned to ten treatment groups (n = 15): no treatment/negative control (C), NaF, APF, fluoride varnish (FV), CPP-ACP, laser (L), L + NaF, L + APF, L + FV, L + CPP-ACP. The microhardness values were measured after the treatments and the pH cycle...
November 16, 2018: Lasers in Medical Science
Yunmei Dong, Yuxin Chen, Yan Tao, Yilong Hao, Lu Jiang, Hongxia Dan, Xin Zeng, Qianming Chen, Yu Zhou
A series of studies are dedicated to research the clinical outcomes of oral leukoplakia (OLK) treated with carbon dioxide laser (CO2 laser); however, the results vary from studies especially related to recurrence and malignant transformation. Hence, we performed this meta-analysis to precisely evaluate the malignant transformation of OLK dealt with CO2 laser and investigate the association between its malignant transformation and kinds of related risk factors, such as gender, clinical classification, long duration of leukoplakia, and degree of epithelial dysplasia and lesion regions...
November 15, 2018: Lasers in Medical Science
M Venosa, E Romanini, R Padua, S Cerciello
The aim of the study was to compare the effect of high-intensity laser therapy (HILT) and a combination of ultrasound (US) treatment and transcutaneous nerve stimulation (TENS) on pain, range of motion (ROM), and functional activity in patients with cervical spondylosis (CS). A total of 84 patients with a mean age of 51.54 years (52 women and 32 men) affected by CS were enrolled in this study. Patients were randomly divided into two groups. In group A (42 subjects), patients received 12 sessions of HILT plus exercise, while in group B (42 subjects), they received a combination of US, TENS, and exercise...
November 15, 2018: Lasers in Medical Science
Marlon da-Palma-Cruz, Rodrigo Fernando da Silva, Dhuane Monteiro, Hassan Mohamed Mohamed Abdel Rehim, Caren Cristina Grabulosa, Ana Paula Ligeiro de Oliveira, Adriana Lino-Dos-Santos-Franco
Sepsis is a big health problem and one of the most common causes of acute lung injury (ALI) leading to high mortality. Pro-resolving mediators play an important role in abrogating the inflammation and promoting tissue homeostasis restoration. ALI treatment is still a clinical health problem, so new therapies are needed. Here, we evaluated the effect of photobiomodulation treatment on the resolution process of ALI induced by lipopolysaccharide (LPS). Male Balb/c mice were submitted to LPS (ip) or vehicle and irradiated or not with light emitting diode (LED) 2 and 6 h after LPS or vehicle injection, and the parameters were investigated 3 and 7 days after the injections...
November 15, 2018: Lasers in Medical Science
Felix Krause, Conny Köhler, Claudia Rüger, Kyung Jin Park, Dirk Ziebolz, Hartmut Schneider, Rainer Haak
The aim of this study was to evaluate the ability of spectral-domain optical coherence tomography (SD-OCT) to display the roof of the pulp chamber and to estimate the residual dentin thickness (RDT) of the pulp complex. The roots of 20 extracted human molars were embedded in epoxy resin, and crowns were longitudinally sectioned in the mesial-distal direction, exposing the pulp chamber. The coronal part of the crown was removed up to an RDT to the pulp chamber roof of 2 mm. Samples were imaged by SD-OCT from coronal view and by light microscopy (LM) in the sagittal plane...
November 13, 2018: Lasers in Medical Science
Akash Verma, Soon Keng Goh, Dessmon Y H Tai, Ai Ching Kor, John Abisheganaden, Zin Nge Nge Sein, Wee See Yap, Alan W K Ng
We compared the outcome of Nd:YAG laser therapy with stent placement for malignant central airway obstruction (CAO) at our center over a 10-year period. This is a retrospective review of patients undergoing Nd:YAG laser therapy or self-expanding metal stent (SEMS) placement for malignant CAO between November 2007 and October 2017. Seventy-two patients were recanalized for malignant CAO. The median (range) age was 63 (23-86) years, with 49 (68%) males. Patients underwent either laser therapy alone (N = 36), stent placement alone (N = 30), or both (N = 6)...
November 12, 2018: Lasers in Medical Science
Minh Nguyet Luong, Masayuki Otsuki, Yasushi Shimada, Thwe Zin Ei, Yasunori Sumi, Junji Tagami
The objective of this study was to evaluate the bleaching effect of light sources with various wavelengths using 30% hydrogen peroxide (HP) in vitro. The hematoporphyrin-stained paper was bleached with HP and irradiated for 10 min using LED light sources with 265, 310, 365, 405, or 450 nm respectively. In control group, HP was applied for 10 min without light irradiation. The bleaching procedure was repeated two times. The L*a*b* values of the samples before bleaching and after each bleaching step were measured using a colorimeter...
November 12, 2018: Lasers in Medical Science
Dimitrios Dionysopoulos, Kosmas Tolidis, Dimitrios Strakas, Thrasyvoulos Sfeikos
The purpose of this in vitro study was to evaluate the effect of a clinical preventive treatment using Er,Cr:YSGG laser irradiation on bovine enamel susceptibility after erosive challenge. Twelve sound bovine incisors were used and twenty-four enamel specimens were prepared in total. Two experimental groups (n = 12) were assigned as follows: Group 1 was the control group and in Group 2, the enamel specimens were irradiated with an Er,Cr:YSGG (2780 nm) laser system for 20 s, with average output power of 0...
November 10, 2018: Lasers in Medical Science
Suryani D Astuti, Putri S Puspita, Alfian P Putra, Andi H Zaidan, Mochamad Z Fahmi, Ardiyansyah Syahrom, Suhariningsih
Candida albicans is a normal flora caused fungal infections and has the ability to form biofilms. The aim of this study was to improve the antifungal effect of silver nanoparticles (AgNPs) and the light source for reducing the biofilm survival of C. albicans. AgNPs were prepared by silver nitrate (AgNO3 ) and trisodium citrate (Na3 C6 H5 O7 ). To determine the antifungal effect of treatments on C. albicans biofilm, samples were distributed into four groups; L + P+ was treatment with laser irradiation and AgNPs; L + P- was treatment with laser irradiation only; L - P+ was treatment with AgNPs only (control positive); L - P- was no treatment with laser irradiation or AgNPs (control negative)...
November 9, 2018: Lasers in Medical Science
Da-Li Jiang, Zheng Yang, Guo-Xiong Liu, Kaijie Wu, Jinhai Fan, Dapeng Wu, Lei Li, Xinyang Wang, Peng Guo, Liyue Mu, Dalin He
Low-power blue laser allows clean cutting with little bleeding and no undesired coagulations in adjacent tissues; however, studies on high-power blue laser soft tissue ablation properties, including vaporization and coagulation, have not been reported yet. The purpose of this study is to evaluate and analyze the ablation efficacy and coagulation properties of bladder epithelium tissues with a 30-W 450-nm wavelength blue laser. Well-designed ex vivo experiments compared blue laser and 532-nm LBO green laser, both with laser power up to 30 W, for porcine bladder tissue vaporization and coagulation at different experimental parameter settings...
November 8, 2018: Lasers in Medical Science
Maria Clara Pavie, Mariana Robatto, Milena Bastos, Sibele Tozetto, Andrea Vilas Boas, Salvatore Giovanni Vitale, Patrícia Lordelo
A healthy female genital mucosa has an ecosystem that remains in balance through interactions between endogenous and exogenous factors. The light-emitting diode (LED) is a device that emits light at different wavelengths, with varying color and effects. Blue light in humans is most commonly used for antimicrobial purposes and has been already applied to treat facial acne and gastric bacteria. Although blue LED therapy in humans has been reported, its properties against vaginal infections have not yet been investigated...
November 7, 2018: Lasers in Medical Science
Adenilson de Souza da Fonseca
Low power lasers have been used successfully for treatment of many diseases in soft and bone tissues. Basic and clinical researches have developed quickly being the scientific basis to therapeutic protocols based on these lasers. However, there are difficulties to compare experimental and clinical results obtained from different researchers because a complicated and intricate list of physical and biological parameters should be checked before the irradiation procedures as well as part of these parameters are omitted or inaccurately reported...
November 6, 2018: Lasers in Medical Science
Ali Moradi, Yashar Kheirollahkhani, Payam Fatahi, Mohammad-Amin Abdollahifar, Abdollah Amini, Parvaneh Naserzadeh, Khadijah Ashtari, Seyed Kamran Ghoreishi, Sufan Chien, Fatemehalsadat Rezaei, Mohammadjavad Fridoni, Mohammad Bagheri, Sudabeh Taheri, Mohammad Bayat
Here, we examined the combined effect of pulse wave photobiomodulation (PBM) with curcumin-loaded superparamagnetic iron oxide (Fe3 O4 ) nanoparticles (curcumin), in an experimental mouse model of acute skin wound. Thirty male adult mice were randomly allocated into 5 groups. Group 1 was served as the control group. Group 2 was a placebo and received distilled water, as a carrier of curcumin. Group 3 received laser (890 nm, 80 Hz, 0.2 J/cm2 ). Group 4 received curcumin by taking four injections around the wound...
November 5, 2018: Lasers in Medical Science
Anandbir Singh Bath, Vishal Gupta
Photobiomodulation (PB) is a utilization of low-level laser therapy (LLLT) in the far red (R) to near infrared (NIR) spectrum (600-1000 nm) to wield its therapeutic effects. To explore the therapeutic potential of biomodulation of different tissues, LLLT has been extensively researched, especially in the light of its very low side effect profile. We believe there is an opportunity to unearth its dynamic effects on the coronaries which can be promising for the patients with chronic stable angina. NIR treatment of the heart may be protective on patients after acute myocardial infarction or on ischemic heart conditions that are not accessible to current revascularization procedures...
October 30, 2018: Lasers in Medical Science
Brenda Ferreira Arantes, Laura de Oliveira Mendonça, Regina Guenka Palma-Dibb, Juliana Jendiroba Faraoni, Denise Tornavoi de Castro, Vinícius Rangel Geraldo-Martins, Cesar Penazzo Lepri
This in vitro study evaluated the influence of the Er,Cr:YSGG laser, associated or not to desensitizing agents, in the prevention of acid erosion in bovine root dentin. Eighty dentin specimens were selected and divided into eight groups (n = 10): G1: negative control; G2: positive control (5% fluoride varnish-FV); G3: Er,Cr:YSGG laser; G4: FV + laser; G5: 3% potassium oxalate; G6: 3% potassium oxalate + laser; G7: biphasic calcium silicate/phosphate gel (gel); G8: gel + laser. Laser parameters: 0...
October 29, 2018: Lasers in Medical Science
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