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Lasers in Medical Science

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https://www.readbyqxmd.com/read/30232646/photobiomodulation-is-associated-with-a-decrease-in-cell-viability-and-migration-in-oral-squamous-cell-carcinoma
#1
Tatiana Dias Schalch, Maria Helena Fernandes, Maria Fernanda Setúbal Destro Rodrigues, Douglas Magno Guimarães, Fabio Daumas Nunes, João Costa Rodrigues, Mônica Pereira Garcia, Raquel Agnelli Mesquita Ferrari, Sandra Kalil Bussadori, Kristianne Porta Santos Fernandes
The treatment of squamous cell carcinoma (SCC) involves surgery, chemotherapy, and/or radiotherapy, which can cause mucositis (inflammation of the oral mucosa that causes considerable pain and can compromise the continuity of oncological treatment). Photobiomodulation (PBM) has been successfully used in the treatment of mucositis, but doubts arise regarding the use of laser for areas in which tumor cells may remain. In this study, the effect of PBM on the viability, mitochondrial activity, proliferation, apoptosis, and migration of cells derived from oral SCC was evaluated...
September 19, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/30232645/effect-of-photobiomodulation-on-neural-differentiation-of-human-umbilical-cord-mesenchymal-stem-cells
#2
Hongli Chen, Hongjun Wu, Huijuan Yin, Jinhai Wang, Huajiang Dong, Qianqian Chen, Yingxin Li
Photobiomodulation therapy (PBMT) can enhance the mesenchymal stem cell (MSC) proliferation, differentiation, and tissue repair and can therefore be used in regenerative medicine. The objective of this study is to investigate the effects of photobiomodulation on the directional neural differentiation of human umbilical cord mesenchymal stem cells (hUC-MSCs) and provide a theoretical basis for neurogenesis. hUC-MSCs were divided into control, inducer, laser, and lasers combined with inducer groups. A 635-nm laser and an 808-nm laser delivering energy densities from 0 to 10 J/cm2 were used in the study...
September 19, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/30229346/effects-and-parameters-of-the-photobiomodulation-in-experimental-models-of-third-degree-burn-systematic-review
#3
Carlos Alberto Ocon, Solange Almeida Dos Santos, Jheniphe Rocha Caires, Marcelo Ferreira Duarte de Oliveira, Andrey Jorge Serra, Ernesto Cesar Leal-Junior, Paulo de Tarso Camillo de Carvalho
This systematic review was performed to identify the role of photobiomodulation therapy in experimental models of third-degree burns used to induce oxidative stress. EMBASE, PubMed, and CINAHL databases were searched for studies published between January 2003 and January 2018 on the topics of photobiomodulation therapy and third-degree burns. Any study that assessed the effects of photobiomodulation therapy in animal models of third-degree burns was included in the analysis. A total of 17 studies were selected from 1182 original articles targeted on photobiomodulation therapy and third-degree burns...
September 18, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/30218348/the-effects-of-photobiomodulation-therapy-on-mouse-pre-osteoblast-cell-line-mc3t3-e1-proliferation-and-apoptosis-via-mir-503-wnt3a-pathway
#4
Qiushi Li, Chen Li, Si Xi, Xianjing Li, Lina Ding, Meihua Li
Photobiomodulation therapy (PBMT) has been demonstrated as regulating osteoblast proliferation. MicroRNAs (miRNAs) are involved in various pathophysiologic processes in osteoblast, but the role of miRNAs in the PBMT-based promotion of osteoblast proliferation remains unclear. This study aimed to investigate the effects of PBMT treatment (3.75 J/cm2 ) on mouse pre-osteoblast cell line MC3T3-E1 proliferation and apoptosis via the miR-503/Wnt3a pathway; meanwhile, detect the expressions of miR-503 and Wnt3a after PBMT treatment and the role of miR-503 in regulating Wnt signaling molecules Wnt3a, β-catenin, Runx2, apoptotic proteins caspase-3, and Bcl-2 in vitro...
September 15, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/30215185/er-cr-ysgg-laser-associated-with-acidulated-phosphate-fluoride-gel-1-23-f-for-prevention-and-control-of-dentin-erosion-progression
#5
S J C Bezerra, L R Trevisan, I E L Viana, R M Lopes, D L Pereira, A C C Aranha, Taís Scaramucci
To evaluate the effect of Er,Cr:YSGG laser, associated with fluoride application, on the prevention/control of dentin erosion. Dentin slabs were embedded in acrylic resin, flattened, and polished. Half of the specimens were previously eroded (10 min immersion in 1% citric acid solution) and half were kept sound. The specimens (n = 10 each substrate) were randomly allocated into the experimental groups, according to the following treatments: control (no treatment); APF gel (1.23% F, 1 min); Er,Cr:YSGG laser irradiation (P1: 0...
September 13, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/30215184/evaluation-of-theranostic-perspective-of-gold-silica-nanoshell-for-cancer-nano-medicine-a-numerical-parametric-study
#6
Xiao Xu, Yildiz Bayazitoglu, Andrew Meade
Using gold-silica nanoshell as a reference nano-agent, this work has performed preliminary numerical parametric study to investigate the feasibility and if feasible the efficiency of using a single nano-agent to achieve theranostic goals. In total, seven generics of gold-silica nanoshells have been tested including the R[50,10] (radius of the silica core is 50 nm and thickness of the gold shell is 10 nm), R[40,15], R[55,25], R[40,40], R[75,40], R[104,23], and R[154,24] nanoshells. A planar tissue model has been constructed as the platform for parametric study...
September 13, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/30215183/temperature-and-depth-evaluation-of-the-in-vitro-effects-of-femtosecond-laser-on-oral-soft-tissue-with-or-without-air-cooling
#7
Wenjun Li, Jianqiao Zheng, Yaopeng Zhang, Fusong Yuan, Peijun Lyu
Femtosecond laser is an effective and safe tool in many surgeries, but the studies of its effect on oral soft tissue ablation are insufficient. This study aimed to investigate the effect of soft tissue ablation with a 1030-nm femtosecond laser on temperature and depth. Twenty Sprague-Dawley rat tongue specimens were obtained and flat-mounted. The 1030-nm femtosecond laser was controlled by a computer system, with a set distance of 4.7 mm between the laser aperture and soft tissue surfaces. Ten specimens were ablated for > 1 min with or without air-cooling for temperature measurement, while the other 10 specimens were ablated for depth measurements, using the following parameters: (i) 3 W, 2000 mm/s; (ii) 3 W, 4000 mm/s; (iii) 5 W, 2000 mm/s; (iv) 5 W, 4000 mm/s; (v) 8 W, 2000 mm/s; (vi) 8 W, 4000 mm/s...
September 13, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/30196440/visualize-and-quantify-the-structural-alteration-of-the-rat-spinal-cord-injury-based-on-multiphoton-microscopy
#8
Chenxi Liao, Xiaoqin Zhu, Linquan Zhou, Zhenyu Wang, Wenge Liu, Jianxin Chen
The development of imaging technique to visualize and quantify the structural alteration of the spinal cord injury (SCI) may lead to better understanding and treatments of the injuries. In this work, multiphoton microscopy (MPM) based on two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) was tentatively applied to quantitatively visualize the cellular microstructures of SCI to demonstrate the feasibility and superiority of MPM in SCI imaging. High-contrast MPM images of normal and injured spinal cord tissue were obtained for comparison...
September 9, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/30194553/clinical-effectiveness-of-multi-wavelength-photobiomodulation-therapy-as-an-adjunct-to-extracorporeal-shock-wave-therapy-in-the-management-of-plantar-fasciitis-a-randomized-controlled-trial
#9
Mary Kamal Nassif Takla, Soheir Shethata Rezk-Allah Rezk
The goal of our study was to investigate the cumulative effect of combining medium-energy extracorporeal shock wave therapy (ESWT) and photobiomodulation therapy (PBMT), as well as to compare between their relative effectiveness in the management of plantar fasciitis (PF). One hundred twenty participants with chronic PF, more than 6 months with failure to respond to conservative treatment, were randomly assigned into four equal groups. Participants received either ESWT with PBMT, ESWT (once a week), PBMT (three times a week), or sham-PBMT (three times a week) for three consecutive weeks...
September 7, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/30191343/blue-laser-imaging-with-acetic-acid-enhancement-improved-the-detection-rate-of-gastric-intestinal-metaplasia
#10
Honglei Chen, Xiaobin Wu, Yanan Liu, Qiuning Wu, Yi Lu, Chujun Li
Our aim was to evaluate the ability of blue laser imaging (BLI) combined with acetic acid (BLI-AA) to detect gastric intestinal metaplasia (GIM). Participants undergoing gastroscopy from July 2017 to February 2018 in our hospital were enrolled prospectively. The abilities of white light imaging endoscopy, BLI endoscopy, and BLI-AA to detect GIM were compared. One hundred six patients undergoing gastroscopy met the inclusion criteria. GIM was diagnosed in 41 patients. For BLI-AA, the sensitivity, specificity, positive predictive, and negative predictive values were 85...
September 6, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/30178432/efficacy-of-high-intensity-laser-therapy-in-comparison-with-conventional-physiotherapy-and-exercise-therapy-on-pain-and-function-of-patients-with-knee-osteoarthritis-a-randomized-controlled-trial-with-12-week-follow-up
#11
Ahmad Nazari, Azar Moezy, Parisa Nejati, Ali Mazaherinezhad
Knee osteoarthritis (KOA) is one of the most common musculoskeletal disorders causing pain and functional impairment. The purpose of the study is to compare the effects of high-intensity laser therapy (HILT), conventional physical therapy (CPT), and exercise therapy (ET) on pain and function in patients with KOA. The study was designed as an assessor-blind randomized controlled trial. Ninety-three patients (aged between 50 and 75 years) with proved KOA were included and randomly allocated into three groups, and received 12 sessions of HILT, CPT, or ET...
September 3, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/30171442/the-effect-of-photobiomodulation-on-total-amount-of-substance-p-in-gingival-crevicular-fluid-placebo-controlled-randomized-clinical-trial
#12
Ezgi Doğanay Yıldız, Hakan Arslan, Serhat Köseoğlu, Taner Arabacı, Dursun Anıl Yıldız, Levent Savran
To investigate the effect of photobiomodulation (PBM) and placebo on total amount of substance P in gingival crevicular fluid (GCF) pre- and postoperatively. Twenty-six patients having tooth with symptomatic apical periodontitis were enrolled in this study. GCF was collected preoperatively. The patients were assigned into two groups (n = 13), as follows: placebo and PBM. Sampling was repeated 7 days after root canal treatment. Two independent samples T test was used for analyzing of the differences between preoperative and postoperative substance P levels in GCF (p = ...
August 31, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/30171441/a-retrospective-comparison-of-thulium-laser-en-bloc-resection-of-bladder-tumor-and-plasmakinetic-transurethral-resection-of-bladder-tumor-in-primary-non-muscle-invasive-bladder-cancer
#13
Kewei Li, Yongzhi Xu, Mingyue Tan, Shujie Xia, Zhonghua Xu, Dongliang Xu
Bladder cancer is currently considered the most common malignancy of the urinary tract. Thulium laser en bloc resection of bladder tumor (TmLRBT) and plasmakinetic transurethral resection of bladder tumor (PK-TURBT) are two alternative common procedures used in our department to manage patients with primary non-muscle invasive bladder cancer (NMIBC) over the past decade. In this work, the safety and efficacy of TmLRBT were retrospectively compared to those of PK-TURBT in patients with primary NMIBC. From January 2013 to December 2015, 256 patients diagnosed with primary NMIBC were selected for this retrospective study...
August 31, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/30145725/photobiomodulation-with-808-nm-diode-laser-light-promotes-wound-healing-of-human-endothelial-cells-through-increased-reactive-oxygen-species-production-stimulating-mitochondrial-oxidative-phosphorylation
#14
Andrea Amaroli, Silvia Ravera, Francesca Baldini, Stefano Benedicenti, Isabella Panfoli, Laura Vergani
Photobiomodulation of cells using near-infrared (NIR) monochromatic light can affect cell functions such as proliferation, viability, and metabolism in a range of cell types. Evidence for the effects of near-infrared light on endothelial cells has been reported, but the studies were mainly performed using VIS light emitted by low-energy lasers, because NIR wavelengths seemed negatively stimulate these cells. Cell viability, free radical-induced oxidative stress, NF-κB activation, nitric oxide release, mitochondrial respiration, and wound healing repair were assessed in human endothelial cells (HECV) irradiated with 808-nm diode laser light (laser setup = 1 W/cm2 , 60 s, 60 J/cm2 , CW vs measured energy parameter = 0...
August 25, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/30145724/effects-of-nd-yag-laser-irradiation-on-the-growth-of-candida-albicans-and-streptococcus-mutans-in-vitro-study
#15
Kinga Grzech-Leśniak, Joanna Nowicka, Magdalena Pajączkowska, Jacek Matys, Maria Szymonowicz, Piotr Kuropka, Zbigniew Rybak, Maciej Dobrzyński, Marzena Dominiak
The purpose of this study was to evaluate the effects of Nd:YAG laser with flat-top handpiece on the in vitro growth of Candida albicans and Streptococcus mutans. The incidence of C. albicans (opportunistic commensal) and S. mutans (facultatively anaerobic) infections is increasing, despite available treatments. Cultures of Streptococcus mutans and Candida albicans were irradiated using Nd:YAG laser (LightWalker, Fotona) with flat-top handpiece (Genova, LightWalker, Fotona) at the following parameters: group G1: 0...
August 25, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/30143925/amph-1-is-a-tumor-suppressor-of-lung-cancer-by-inhibiting-ras-raf-mek-erk-signal-pathway
#16
Haifeng Yang, Zhenzhou Wan, Cheng Huang, Huabin Yin, Dianwen Song
Amphiphysin 1 (AMPH-1) is a nerve terminal-enriched protein and it is a 128-kD protein with three identified functional domains. Some studies found that AMPH-1 was a dominant autoantigen associated with breast cancer and melanoma. However, its function in lung cancer is unknown. Here, we showed that AMPH-1 knockdown dramatically increased cell proliferation, attenuated cell apoptosis, and promoted cell cycle progression in human lung cancer cells. In vivo xenograft studies confirmed that the AMPH-1-knockdown cells were more tumorigenic than the controls...
August 24, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/30143924/photo-modulation-of-zinc-phthalocyanine-treated-breast-cancer-cell-line-zr-75-1-inhibited-the-normal-tumor-activity-in-vitro
#17
Arezoo Rezaie Nezhad Zamani, Mohammad Reza Mashayekhi, Mahdieh Farhoudi Sefidan Jadid, Yousef Faridvand, Habib Tajalli, Reza Rahbarghazi
Regarding post-complication of convenient therapies against breast cancer, the emergence of effective approaches is essential. Photodynamic therapy is touted as a novel invasive therapeutic approach by the application of a photosensitizer promoted by laser irradiation. This study aimed to investigate the combined regime of low-level laser irradiation with zinc phthalocyanine in human breast cancer ZR-75-1 cell line. Cells were treated with 0.01 and 5 μg/ml of ZnPc for 24 h and exposed to radiation (70 mW) for 60 s...
August 24, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/30143923/effectiveness-of-photopneumatic-technology-a-descriptive-review-of-the-literature
#18
Ali Rajabi-Estarabadi, Siri Choragudi, Isabella Camacho, Kevin J Moore, Jonette E Keri, Keyvan Nouri
Usage of photopneumatic technology has recently increased for treatment of different skin conditions such as acne, keratosis pilaris (KP), and rosacea. Photopneumatic devices combine gentle negative pressure with broad band pulsed light simultaneously to attack multiple targets in the skin for better treatment outcomes. In this literature review, we evaluate the efficacy of photopneumatic therapy on treatment of acne, keratosis pilaris (KP), and rosacea.
August 24, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/30141135/monitoring-tumor-progression-by-mapping-skin-microcirculation-with-laser-doppler-flowmetry
#19
Tanvi Pedanekar, Rohit Kedare, Amit Sengupta
Laser Doppler flow meter is a safe, useful, and noninvasive tool to monitor microvascular blood flow. It measures the changes in flow either over time or differences in flow over an area of skin or other exposed tissue. The primary function of LDF is to produce blood perfusion output signal that is proportional to the red blood cell perfusion (or flux). A simple analog-based laser Doppler flow meter is used to record the biosignals arising from the microvascular network of a surface lesion in the face of a human being...
August 24, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/30136213/application-of-continuous-wave-photoacoustic-sensing-to-red-blood-cell-morphology
#20
Abhijeet Gorey, Deblina Biswas, Anshu Kumari, Sharad Gupta, Norman Sharma, George C K Chen, Srivathsan Vasudevan
The feasibility of continuous wave laser-based photoacoustic (CWPA) response technique in detecting the morphological changes in cells during the biological studies, through the features extracted from CWPA signal (i.e., amplitude) is demonstrated here. Various hematological disorders (e.g., sickle cell anemia, thalesemia) produce distinct changes at the cellular level morphologically. In order to explore the photoacoustic response technique to detect these morphological changes, we have applied CWPA technique onto the blood samples...
August 22, 2018: Lasers in Medical Science
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