journal
https://read.qxmd.com/read/7849542/the-effect-of-fluoride-therapy-on-blood-chemistry-parameters-in-osteoporotic-females
#21
COMPARATIVE STUDY
R Jackson, S Kelly, T Noblitt, W Zhang, A Dunipace, Y Li, G Stookey, B Katz, E Brizendine, S Farley
To determine the potential adverse effects, if any, of long-term fluoride ingestion in humans, samples were collected from 25 adult females taking daily doses of fluoride (mean, 23 mg elemental F) for the treatment of osteoporosis and from 38 osteoporotic female controls. Patients in the fluoride group had been receiving therapy for approximately 18 months with a mean duration of 4.2 years and had serum fluoride values of at least 10 mumol/l. Laboratory analyses for fluoride were conducted on plasma, urine and drinking water samples collected from each panelist...
October 1994: Bone and Mineral
https://read.qxmd.com/read/7849541/bone-mineralization-in-children-and-adolescents-with-a-milk-allergy
#22
JOURNAL ARTICLE
R C Henderson, P R Hayes
To evaluate the correlation between dietary calcium intake and mineralization of the immature skeleton 55 children and adolescents aged 5-14 years (mean, 9.5 years) with a positive radioallergosorbent test (RAST) for serum antibodies to cow's milk protein were evaluated. Bone mineral density (BMD) in the lumbar spine and proximal femurs were measured by dual energy X-ray absorptiometry. BMD at each site for each subject was converted to an age-adjusted Z score based on our own series of 95 normal pediatric controls...
October 1994: Bone and Mineral
https://read.qxmd.com/read/7819832/1-25-dihydroxyvitamin-d3-and-osteocalcin-in-maternal-and-fetal-guinea-pigs
#23
JOURNAL ARTICLE
J Verhaeghe, K Allewaert, E Van Herck, R Van Bree, F A Van Assche, R Bouillon
Maternal and fetal 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) and osteocalcin were measured in guinea pigs, to examine their potential use as animal models for fetal bone development and calcium homeostasis. Measurements were performed on days 42, 57 and 63 of gestation. Maternal serum total 1,25(OH)2D3 concentrations were increased only at the end of gestation (day 63). However, because the vitamin D binding protein (DBP) and albumin levels were decreased by 35-50% from day 42 onwards, the unbound 1,25(OH)2D3, calculated as the 1,25(OH)2D3/DBP molar ratio, was increased before day 63...
September 1994: Bone and Mineral
https://read.qxmd.com/read/7819831/the-receptor-metabolism-and-effects-of-androgen-in-osteoblastic-mc3t3-e1-cells
#24
JOURNAL ARTICLE
Y Nakano, I Morimoto, O Ishida, T Fujihira, A Mizokami, A Tanimoto, N Yanagihara, F Izumi, S Eto
We investigated the androgen receptor (AR), metabolism and effects of androgens in osteoblastic MC3T3-E1 cells. AR was proved as a transcript of a 10-kb mRNA and as a 110-kDa protein. An immunocytochemical study showed that AR was located mainly in the nuclei. Specific binding of [3H]DHT was observed in both the nuclear and cytosol fractions. MC3T3-E1 cells possessed approximately 1190 binding sites per cell and most of the sites (1150 sites) situated in the nucleus. The apparent Kd value in the nuclear fraction was 1...
September 1994: Bone and Mineral
https://read.qxmd.com/read/7819830/effect-of-cyclosporine-a-on-normal-human-osteoblasts-in-vitro
#25
JOURNAL ARTICLE
M Nàcher, J Aubia, S Serrano, M L Mariñoso, J Hernández, J Bosch, A Díez, J M Puig, J Lloveras
UNLABELLED: Cyclosporin-A (CsA) inhibits in vitro proliferation of non-human tumour-cloned osteoblasts. Our aims were to study the direct effect of CsA on proliferation of normal human osteoblast (NHOb) cultures and to ascertain whether CsA-treated patients' sera (CsATPS) may exert effects on the osteoblast which differ from the direct effects of CsA. We studied tritiated thymidine ([3H]thymidine) incorporation in NHOb cultures incubated with (a) increasing CsA concentrations (1.2 to 4800 ng/ml), (b) the same concentrations as in the previous experiment but with the addition of 20% fetal calf serum (FCS) or 20% normal human serum (NHS), (c) 40% NHS or 40% CsATPS...
September 1994: Bone and Mineral
https://read.qxmd.com/read/7819829/effect-of-1-hydroxyethylidene-1-1-bisphosphonate-on-membrane-mediated-calcium-phosphate-formation-in-model-liposomal-suspensions
#26
JOURNAL ARTICLE
D Skrtic, E D Eanes
The bisphosphonate, 1-hydroxyethylidene-1,1-bisphosphonate (HEBP), was examined for its effect on calcium phosphate precipitation in pH 7.4, 22 degrees C suspensions of 7:2:1 PC:phosphatidylcholine (PC):dicetylphosphate (DCP):cholesterol (Chol) and 7:1:1 PC:phosphatidylserine (PS):Chol liposomes. HEBP (0.5-50 mumol/l) in the suspending medium had little, if any, effect on precipitation that formed inside phosphate-rich (50 mmol/l) aqueous interiors of liposomes as a result of ionophore (X-537A) driven 2.25 mmol/l Ca2+ influxes from the medium...
September 1994: Bone and Mineral
https://read.qxmd.com/read/7819828/age-dependence-of-the-normal-abnormal-difference-of-bone-mineral-density-in-osteoporotic-women
#27
JOURNAL ARTICLE
A Bagur, E Vega, C Mautalen
Bone mineral density (BMD) is the major factor in bone strength and in the risk of suffering osteoporotic fractures. The aim of this study was to examine the normal/abnormal difference for antero-posterior (AP) spine, lateral spine, proximal femur and total body BMD to assess if age influences discrimination at three different decades between 50 and 80 years of age. The BMD was determined in 61 control women and 60 osteoporotic women (at least one vertebral wedge fracture readily visible in the lateral X-rays of the thoracic or lumbar spine)...
September 1994: Bone and Mineral
https://read.qxmd.com/read/7819827/urinary-excretion-of-pyridinolines-crosslinks-measured-by-immunoassay-and-hplc-techniques-in-normal-subjects-and-in-elderly-patients-with-vitamin-d-deficiency
#28
JOURNAL ARTICLE
S Kamel, M Brazier, C Picard, F Boitte, L Samson, G Desmet, J L Sebert
Hydroxylysylpyridinoline (HP) and lysylpyridinoline (LP) are specific constituents of mature skeletal collagens excreted in urine. Their measurement represents a sensitive index of bone resorption. In this study, we have measured urinary excretion of pyridinolines crosslinks by immunoassay (ELISA) and HPLC methods in 80 patients with different bone resorption rates. We chose a sample of 44 healthy adults (30 men and 14 women) and a sample of 36 elderly patients (7 men and 29 women) presenting a secondary hyperparathyroidism due to a vitamin D deficiency...
September 1994: Bone and Mineral
https://read.qxmd.com/read/7710500/histomorphometric-and-mineral-density-fractionation-studies-of-lumbar-vertebrae-of-intact-and-ovariectomized-ovx-monkeys
#29
LETTER
C P Jerome, C S Carlson, M J Jayo, T C Register, D S Weaver, C J Lees, M R Adams
No abstract text is available yet for this article.
September 1994: Bone and Mineral
https://read.qxmd.com/read/7994190/effects-of-two-novel-bisphosphonates-on-bone-cells-in-vitro
#30
COMPARATIVE STUDY
C E Evans, I P Braidman
Bisphosphonates are now widely used in the treatment of bone diseases, particularly where there is uncontrolled bone resorption, as they are known to be potent inhibitors of osteoclasis. It is still unclear whether the bisphosphonates act by inhibiting osteoclast maturation or by blocking the mechanism of bone resorption, and little is known of their effects on osteoblasts. Recent studies with 3-amino-1, hydroxypropylidene-1,1-bisphosphonic acid (APD) in the treatment of osteolytic metastases in breast cancer have suggested that APD may affect osteoblasts directly...
August 1994: Bone and Mineral
https://read.qxmd.com/read/7994189/in-vivo-effect-of-17-beta-estradiol-on-intestinal-calcium-absorption-in-rats
#31
COMPARATIVE STUDY
B H Arjmandi, B W Hollis, D N Kalu
Previously we reported that intestinal cells contain estrogen receptors, and that 17 beta-estradiol enhanced calcium uptake by these cells in vitro. The current study was undertaken to examine the in vivo effects of 17 beta-estradiol on intestinal absorption of calcium and phosphorus. Three groups of rats were studied. Group 1 received solvent vehicle. Groups 2 and 3 received 5 micrograms and 40 micrograms 17 beta-estradiol/kg body weight/day, respectively, for 21 days. Hormone and solvent vehicle injections were given subcutaneously...
August 1994: Bone and Mineral
https://read.qxmd.com/read/7994188/normal-ranges-for-bone-loss-rates
#32
COMPARATIVE STUDY
P D Ross, Y F He, J W Davis, R S Epstein, R D Wasnich
We reported previously that the variability in bone loss rates among postmenopausal women decreases dramatically during the first few years of followup. In this paper, we have examined the distributions of bone loss rates measured at the calcaneus, distal radius and proximal radius. The incidence of physical impairment was five times greater among women with bone loss rates faster than 2 S.D. below the mean. Because the rate of change in bone density was skewed at the lower end of the distribution (representing rapid bone loss), the influence of values at the extreme ends of the distribution were statistically removed in order to estimate the normal distribution of bone loss rates...
August 1994: Bone and Mineral
https://read.qxmd.com/read/7994187/evaluation-and-comparison-of-urinary-pyridinium-crosslinks-in-two-rat-models-of-bone-loss-ovariectomy-and-adjuvant-polyarthritis-using-a-new-automated-hplc-method
#33
COMPARATIVE STUDY
C Tordjman, A Lhumeau, P Pastoureau, F Meunier, B Serkiz, J P Volland, J Bonnet
A specific HPLC system was developed to assess urinary excretion of collagen crosslinks (pyridinoline (Pyr) and deoxypyridinoline (D.Pyr)) in two models of osteopenia in rats, ovariectomy and adjuvant polyarthritis. The sensitivity of this method was in the picomolar range. In ovariectomized rats, a specific model of bone resorption, Pyr and D.Pyr levels rose early, reaching a peak 2 weeks after surgery. Both levels remained raised during the whole observation period (6 weeks) with no change in the Pyr/D.Pyr ratio...
August 1994: Bone and Mineral
https://read.qxmd.com/read/7994186/regulation-of-protein-kinase-c-activity-by-phorbol-ester-thrombin-parathyroid-hormone-and-transforming-growth-factor-beta-2-in-different-types-of-osteoblastic-cells
#34
JOURNAL ARTICLE
M P Bos, J M van der Meer, M P Herrmann-Erlee
We investigated the role of protein kinase C (PKC) in osteoblast function using a set of putative PKC modulating factors and an in situ peptide substrate-based kinase assay in different types of osteoblastic cells. Primary calvarial rat osteoblastic cells (ROB) and ROS 17/2.8 osteosarcoma cells showed an equally high PKC activity when a maximal dose of PKC-activating phorbol ester was applied. The osteosarcoma cell line UMR 106-01 showed only 5-10% of this maximal PKC activity. All 3 cell types responded to 10 U/ml thrombin with a 2-fold stimulation of PKC activity...
August 1994: Bone and Mineral
https://read.qxmd.com/read/7994185/effects-of-endurance-exercise-on-bone-histomorphometric-parameters-in-intact-and-ovariectomized-rats
#35
COMPARATIVE STUDY
E I Barengolts, P V Lathon, D J Curry, S C Kukreja
Physical activity is important for maintenance of bone mass. The effects of exercise on bone histomorphometry were studied in 9-month-old intact (INT) and ovariectomized (OVX) rats. The rats were either kept sedentary (SED) or were exercised (EX) on a treadmill at 21 m/min for 1 h/day 5 days/week for 3 months. Bone resorption as well as formation parameters were significantly higher in OVX-SED than in INT-SED rats, indicating increased bone turnover in OVX rats. In OVX rats, lower osteoclast perimeter and number, lower labeled perimeter but higher mineral apposition rate (MAR) and bone formation rate (BFR) were associated with higher trabecular bone in OVX-EX compared with OVX-SED rats...
August 1994: Bone and Mineral
https://read.qxmd.com/read/7994184/time-related-increase-of-biochemical-markers-of-bone-turnover-in-androgen-deficient-male-rats
#36
COMPARATIVE STUDY
D Vanderschueren, I Jans, E van Herck, K Moermans, J Verhaeghe, R Bouillon
Bone loss during androgen deficiency has been associated with accelerated bone turnover and imbalance between bone formation and resorption but the relative increase of both phenomena is not well described. Serum osteocalcin as marker of bone formation and urinary excretion of pyridinoline (PYD) and deoxypyridinoline (DPD) as markers of bone resorption were measured in both orchidectomized (ORCH, n = 8) and sham-operated (SHAM, n = 8) aged (12-month-old) male rats from 2 days before until 66 days after surgery...
August 1994: Bone and Mineral
https://read.qxmd.com/read/7994183/alterations-in-the-expression-of-osteonectin-osteopontin-and-osteocalcin-mrnas-during-the-development-of-skeletal-tissues-in-vivo
#37
JOURNAL ARTICLE
T Nakase, K Takaoka, K Hirakawa, S Hirota, T Takemura, H Onoue, K Takebayashi, Y Kitamura, S Nomura
Heterogeneity in the expression of three members of non-collagenous matrix proteins in osteogenic and chondrogenic development in vivo were investigated by in situ hybridization. Sections of several skeletal tissues from mice at various stages of development were hybridized with digoxigenin-labeled complementary RNA probes encoding osteonectin (Osn), osteopontin (Osp) and osteocalcin (Osc). In calvariae and mandibulae, Osn messenger RNA (mRNA) was detected in cells in pre-osseous and osseous tissues before mineralization...
August 1994: Bone and Mineral
https://read.qxmd.com/read/7950508/bone-density-and-turnover-in-addison-s-disease-effect-of-glucocorticoid-treatment
#38
JOURNAL ARTICLE
M A Valero, M Leon, M P Ruiz Valdepeñas, L Larrodera, M B Lopez, K Papapietro, A Jara, F Hawkins
Osteoporosis is a well-known side-effect of chronic treatment with glucocorticoids. We have studied vertebral bone mineral density (BMD) and biochemical markers of bone metabolism in 30 patients diagnosed of Addison's disease (AD) to determine the effect of long-term replacement treatment with hydrocortisone (30 mg/day) or prednisone (7.5 mg/day). Lumbar bone mineral density was measured with dual energy X-ray absorptiometry in L-1-4 in two occasions, separated by 12 months. BMD in premenopausal women and men with AD was similar to healthy controls and postmenopausal women had slightly lower results...
July 1994: Bone and Mineral
https://read.qxmd.com/read/7950507/dietary-xylitol-prevents-ovariectomy-induced-changes-of-bone-inorganic-fraction-in-rats
#39
JOURNAL ARTICLE
M Svanberg, M Knuuttila
Thirty-six 3-month-old female Wistar rats were labelled with a single intraperitoneal tetracycline injection. Twenty-four animals were subsequently ovariectomized, while the control group of 12 animals underwent sham operations. All animals received the basal Ewos R3 diet and half of the ovariectomized animals (n = 12) were given an additional 5% dietary xylitol supplementation. Three months later, following the collection of blood and urine, the animals were killed by decapitation. The tibiae were detached and prepared for chemical and other studies...
July 1994: Bone and Mineral
https://read.qxmd.com/read/7950506/a-facilitative-role-for-carbonic-anhydrase-activity-in-matrix-vesicle-mineralization
#40
JOURNAL ARTICLE
G R Sauer, B R Genge, L N Wu, J E Donachy
Carbonic anhydrase (CA) which catalyzes the reversible hydrolysis of carbon dioxide is known to be important in osteoclastic bone resorption, however, suggested roles in calcium phosphate mineral formation have not been previously demonstrated. Biochemical evidence is provided for the presence of CA in growth plate matrix vesicles (MV) and the level of activity determined by enzyme assay. Inhibition of CA activity with the specific inhibitor acetazolamide resulted in reduced rates of MV mineralization. Other inhibitor studies showed that MV mineralization was also impaired by 4,4-diisothiocyanatostilbene-2, 2-disulfonic acid (DIDS), a blocker of membrane bicarbonate channels...
July 1994: Bone and Mineral
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