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Biotechnology and Applied Biochemistry

A P Tyunin, N N Nityagovsky, V P Grigorchuk, K V Kiselev
It has previously been shown that exogenous application of p-coumaric acid (CA), a precursor of phenolic compounds, improved stilbene production in cell cultures of Vitis amurensis. This study examines the effect of cinnamic (Cin) and caffeic (Caf) acids, which are also phenolic precursors, on stilbene biosynthesis in the cell cultures. Five stilbenes, t-resveratrol diglucoside, t-piceid (t-resveratrol glucoside), t-resveratrol, t-ε- and t-δ-viniferins, were found in the treated and untreated cells. Cin increased the total stilbene production in the grape cell cultures 2...
March 22, 2017: Biotechnology and Applied Biochemistry
Marzieh Jafari, Mohsen Rezaei, Heibatullah Kalantari, Maryam Tabarzad, Bahram Daraei
DNAzyme and aptamer conjugations have already been used for sensitive and accurate detection of several molecules. In this study, we tested the relationship between conjugation orientation of DNAzyme and Aflatoxin B1 aptamer and their subsequent peroxidase activity. Circular dichroism (CD) spectroscopy and biochemical analysis were used here to difference between these two conjugation patterns. Results showed that DNAzyme-aptamer has more catalytic activity and efficiency than aptamer-DNAzyme. Thereby, DNAzyme-aptamer with its superior efficiency can be used for design and development of more sensitive Aflatoxin B1 DNA based biosensors...
March 22, 2017: Biotechnology and Applied Biochemistry
Agata Wasak, Radosław Drozd, Łukasz Struk, Bartłomiej Grygorcewicz
The aim of this study was to investigate the optimal conditions for the immobilisation and stabilisation of DyP1B Dye-decolourising peroxidases type B (DyP1B) from Pseudomonas fluorescens Pf-5 immobilised in Ca-alginate ferromagnetic beads (Ca-alginate FeBs). The immobilised DyP1B was used in the degradation of the Reactive Blue 5 synthetic dye. The enzyme was successfully entrapped in a Ca-alginate matrix, and showed an encapsulation efficiency of 94%. The concentration of 0.8 mg mL(-1) DyP1B, 2% of alginate and 10 mg mL(-1) magnetite were optimal for immobilisation...
March 21, 2017: Biotechnology and Applied Biochemistry
Shalini Raik, Ajay Kumar, Shalmoli Bhattacharyya
Current advances in medicine have revolutionized the field of regenerative medicine dramatically with newly evolved therapies for repair or replacement of degenerating or injured tissues. Stem cells can be harvested from different sources for clinical therapeutics which include fetal tissues, umbilical cord blood, embryos and adult tissues. Stem cells can be isolated and differentiated into desired lineages for tissue regeneration and cell replacement therapy. However, several loopholes need to be addressed properly before this can be extended for large scale therapeutic application...
March 21, 2017: Biotechnology and Applied Biochemistry
Traian V Chirila, Shuko Suzuki, Chloé Papolla
Fibroin, the major proteinaceous component of the silk fiber produced by larvae of the domesticated silk moth (Bombyx mori), has been widely investigated as a biomaterial for potential applications in tissue engineering and regenerative medicine. Following sol-gel transition, silk fibroin solutions can generate hydrogels that present certain advantages when employed as biomaterials, especially if they are crosslinked. The subject of this study was the self-crosslinking of silk fibroin through a process induced by the enzyme horseradish peroxidase (HRP) in the presence of hydrogen peroxide, a method only recently proposed and scarcely reported...
February 21, 2017: Biotechnology and Applied Biochemistry
Huaiyan Sun, Xinyu Jin, Feng Jiang, Ruifeng Zhang
A Zinc Oxide (ZnO) nanowires/ macroporous Silicon dioxide (SiO2 ) composite was used as support to immobilize horseradish peroxidase (HRP) simply by in-situ cross-linking method. As cross-linker was adsorbed on the surface of ZnO nanowires, the cross-linked HRP was quite different from the traditional cross-linking enzyme aggregates (CLEAs) on both structure and catalytic performance. Among three epoxy compounds diethylene glycol diglycidyl ether (DDE) was the best cross-linker, with which the loading amount of HRP with pI of 5...
February 21, 2017: Biotechnology and Applied Biochemistry
Peipei Lv, Wanggang Tang, Peng Wang, Zhengyu Cao, Guoping Zhu
Isocitrate dehydrogenase (IDH) is a key enzyme at the critical junction between the tricarboxylic acid cycle and the glyoxylate cycle. Most bacteria have only one IDH, while a few of bacteria contain two IDH isozymes. The coexistence of two different type IDHs in one organism was little known. Xylella fastidiosa is a nutritionally fastidious plant pathogen that contains two structurally different IDHs, an NAD(+) -dependent homodimeric IDH (diXfIDH) and an NADP(+) -dependent monomeric IDH (monoXfIDH). Kinetic characterization showed that diXfIDH displayed 206-fold preferences for NAD(+) over NADP(+) , while monoXfIDH showed 13800-fold preferences for NADP(+) over NAD(+) ...
February 21, 2017: Biotechnology and Applied Biochemistry
Carlos A Duarte, Mickel Palomino
Virion infectivity factor (Vif) is a 23 KDa protein which protects HIV-1 from deamination of its proviral DNA by APOBEC3G. The active form of Vif is a multimer that interacts simultaneously with CBF-beta, the Elongin B and C subunits, Cullin 5 and APOBEC3G to form a ubiquitin ligase complex targeting the latter for degradation. Vif clearly represents an attractive target for developing novel antiviral drugs for the therapy of HIV/AIDS, and this goal requires a source of well folded, readily available protein...
February 8, 2017: Biotechnology and Applied Biochemistry
Chang Su, Zhiyang Chen, I-Son Ng
Proteus hauseri ZMd44, a biodecolorizing bacterium, has been known to produce electricity and multi-copper oxidase (Mco-laccase) under copper induction. However, optimization and regulation of production have not been explored. This study is the first attempt to evaluate several parameters on biomass and Mco-laccase production of P. hauseri ZMd44. Through orthogonal experiments with Taguchi's L9 found that P. hauseri ZMd44 was sensitive to pH value. The cells grew relatively quickly at pH 7, thus the biomass and Mco-laccase-production reached 1...
February 1, 2017: Biotechnology and Applied Biochemistry
Jaruwan Siritapetawee, Chutima Talabnin, Jarunee Vanichtanankul, Chomphunuch Songsiriritthigul, Kanjana Thumanu, Chun-Jung Chen, Nantarat Komanasin
In this study, the binding of a glycosylated serine protease (EuP-82) with human fibrinogen was investigated by the isothermal titration calorimetry (ITC). The ITC analysis indicated that the binding of EuP-82 to fibrinogen in the conditions with or without the activator (Ca(2+) ) was an exothermic reaction (dominant negative enthalpy), which tended to be driven by hydrogen bonding and van der Waals interactions. In contrast, the binding of fibrinogen-EuP-82 in the condition with the inhibitor (Zn(2+) ) was an unfavorable endothermic reaction...
February 1, 2017: Biotechnology and Applied Biochemistry
Bin Xia, Yang Zou, Zhiling Xu, Yonggang Lv
Low-intensity pulsed ultrasound (LIPUS) is a non-invasive technique that has been shown to affect the cell proliferation, migration, differentiation, and promote the regeneration of damaged peripheral nerve. Our previous studies had proved that LIPUS can significantly promote the neural differentiation of induced pluripotent stem cells-derived neural crest stem cells (iPSCs-NCSCs) and enhance the repair of rat transected sciatic nerve. To further explore the underlying mechanisms of LIPUS treatment on iPSCs-NCSCs, this study reported the gene expression profiling analysis of iPSCs-NCSCs before and after LIPUS treatment using RNA-sequencing (RNA-Seq) method...
January 26, 2017: Biotechnology and Applied Biochemistry
Meng-Jie Tan, Xue-Wei Cao, Peng-Fei Li, Yi-Zhou Zhai, Yu Zhou, Ye-Jun Liu, Jian Zhao, Fu-Jun Wang
Alpha-momorcharin (α-MMC), a type I ribosome inactivating protein, has attracted a great deal attention because of its antitumor activity. However, the cytotoxicity of α-MMC is limited due to insufficient cellular internalization in cancer cells. To enhance the cytotoxicity of α-MMC, a heparin-binding domain derived from HB-EGF (named HBP) was used as a cell-penetrating peptide and fused to the C-terminus of α-MMC. This novel α-MMC-HBP fusion protein was expressed and purified with a Ni(2+) -resin. The N-glycosidase activity and DNase activity assay indicated that the introduction of HBP did not interfere with the intrinsic bioactivities of α-MMC...
January 16, 2017: Biotechnology and Applied Biochemistry
Yangyun Zhou, Xun Zhou, Qing Li, Junfeng Chen, Ying Xiao, Lei Zhang, Wansheng Chen
Production of major effective metabolites, tanshinones and lithospermic acid B (LAB), was dramatically enhanced by exogenous jasmonate (JA) treatment in Salvia miltiorrhiza. However, the molecular mechanism of such metabolic activation in S. miltiorrhiza has not been elucidated yet. Here, we focused on jasmonate ZIM-domain (JAZ) proteins that act as repressors of JA signaling. Open reading frames of two novel genes, SmJAZ1 and SmJAZ2, from S. miltiorrhiza were amplified according to the annotation of S. miltiorrhiza transcriptome...
January 2017: Biotechnology and Applied Biochemistry
Safa Ben Azoun, Meriem Ben Zakour, Soufien Sghaier, Héla Kallel
Rabies is a fatal disease that can be prevented by vaccination. Different approaches were investigated to develop novel human rabies vaccines with improved features compared to the current available vaccines, among them is the use of heterologous gene expression technology. Here, we describe the expression of the surface rabies virus glycoprotein (RABV-G), which is the major antigen responsible for the induction of protective immunity, in Pichia pastoris. Six transformants were selected according to their gene copy number as determined by real time qPCR...
January 2017: Biotechnology and Applied Biochemistry
Songhe Wang, Zhibin Liu
To improve the hydroxylation efficiency of cytochrome P450 CYP102A3 to substrate n-octane, a previously reported triple-mutant F88V/S188Q/A330V with altered regioselectivity was selected and site-saturation mutagenesis was performed at its P331 site, which is adjacent to one of the active sites A330. Using whole-cell biotransformations to analyze the created mutants, four better mutants P331A, P331T, P331S, and P331V were achieved and exhibited significantly improved conversion rates toward n-octane, which are 33%, 33%, 22% and 28%, respectively, whereas the activity of P331R was greatly reduced and P331K gave almost zero conversion of n-octane...
January 2017: Biotechnology and Applied Biochemistry
Ronak Rafipour, Soheila Kashanian, Sadegh Hashemi, Kobra Omidfar, Jafar Ezzati Nazhad Dolatabadi
Manganese nanoparticles (MnNPs) were created within horse spleen apoferritin (HsAFr) cavity nanotemplates. Transmission electron microscopy revealed the particle size to be 6 nm. Intrinsic fluorescence data showed that the mineralization acted as a quencher of the HsAFr fluorescence, and extrinsic fluorescence data revealed that the hydrophobic binding site at the surface of HsAFr was not changed. Finally, the MnNP-HsAFr was immobilized onto multiwalled carbon nanotubes entrapped into chitosan (CS) matrices by through sequential 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide-N-hydroxysuccinimide and glutaraldehyde coupling...
January 2017: Biotechnology and Applied Biochemistry
Hamid Bashir, Nadeem Ahmed, Mohsin Ahmad Khan, Ahmad Usman Zafar, Saad Tahir, Hina Kanwal, Faidad Khan, Zia Ur Rahman, Tayyab Husnain
Recent advancement in fermentation technologies resulted in the increased yields of recombinant proteins of biopharmaceutical and medicinal importance. Consequently, there is an important task to develop simple and easily scalable methods that can facilitate the production of high-quality recombinant protein. Most of the recent reports described the expression of recombinant human IL-1 receptor antagonist (rhIL-1Ra) in Escherichia coli using isopropyl-β-d-thiogalacto pyranoside (IPTG), a nonmetabolizable and expensive compound, as an expression inducer...
January 2017: Biotechnology and Applied Biochemistry
Catia Algieri, Laura Donato, Lidietta Giorno
Polyvinylidene fluoride (PVDF) membrane surfaces were ad hoc functionalized chemically to make them suitable for enzymatic immobilization. The process was performed by grafting the membrane surface with 1,4-diaminobutane and subsequently by activating it with glutarhaldehyde. The chemico-physical properties of the original PVDF membrane and of the modified membranes were studied by infrared spectroscopy, scanning electron microscopy, and static contact angle measurements. The activated membranes were used as a support for covalent immobilization of tyrosinase...
January 2017: Biotechnology and Applied Biochemistry
Meidi Yan, Lina Zhang, Guoqing Li, Shengwen Xiao, Ji Dai, Xueying Cen
Breast cancer is the most commonly diagnosed cancer and the leading cause of cancer-related death in women globally. Its high morbidity and mortality, as well as its elevated tendency to metastasize to other organs, warrant the urgency to find new biomarkers for breast cancer diagnosis and treatment. The specific roles of long noncoding RNA linc-ITGB1 on cell proliferation and metastasis in breast cancer were explored in this study. The expression of linc-ITGB1 was significantly upregulated in both clinical breast cancer tissues and cultured breast cancer cell lines...
January 2017: Biotechnology and Applied Biochemistry
Xiao-Xin Chen, Wai Ling Lin, Wing Fai Yeung, Tian-He Song, Li-Xing Lao, Yan-Bo Zhang, Wei Meng
Reproducible efficacy assessments of Chinese herbal medicines are largely based on well-established quality control procedures. This study presents a comprehensive quality control procedure for tumor-shrinking decoction (TSD), a 15-herb preparation under study as a potential therapy for uterine fibroids. Morphological, microscopic, and physicochemical authentications were first carried out on individual herbal medicines composing TSD. Contaminant tests on TSD for the presence of heavy metals and pesticide residues were performed by atomic absorption spectrophotometry and gas chromatography-mass spectrometry analysis...
January 2017: Biotechnology and Applied Biochemistry
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