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Biotechnology and Applied Biochemistry

Edgar Edurman García-Silvera, Fernando Martínez-Morales, Brandt Bertrand, Daniel Morales-Guzman, Nashbly Sarela Rosas-Galván, Renato León-Rodríguez, María Del Refugio Trejo-Hernández
In this study, extracellular lipase was produced by Serratia marcescens wild type and three mutant strains. The maximum lipase activity (80 U/mL) was obtained with the SMRG4 mutant strain using soybean oil. Using a 2(2) factorial design the lipase production increased 1.55-fold (124 U/mL) with 4% and 0.05 % soybean oil and Triton X-100, respectively. The optimum conditions for maximum lipase activity were 50°C and pH 8. However, the enzyme was active in a broad range of pH (6-10) and temperatures (5-55°C)...
April 26, 2017: Biotechnology and Applied Biochemistry
Veeresh Juturu, Jin Chuan Wu
Thermophilic Bacillus coagulans JI12 was used to ferment hemicellulose hydrolysate obtained by acid hydrolysis of oil palm empty fruit bunch (EFB) at 50°C and pH6, producing 105.4 g/L of L-lactic acid with a productivity of 9.3 g/L/h by fed batch fermentation under unsterilized conditions. Simultaneous saccharification and fermentation (SSF) was performed at pH5.5 and 50°C to convert both hemicellulose hydrolysate and cellulose-lignin complex in the presence of Cellic Ctec2 cellulases using yeast extract (20 g/L) as the nitrogen source, giving 114...
April 24, 2017: Biotechnology and Applied Biochemistry
A P Tyunin, N N Nityagovsky, V P Grigorchuk, K V Kiselev
It has previously been shown that exogenous application of p-coumaric acid (CA), a precursor of phenolic compounds, improved stilbene production in cell cultures of Vitis amurensis. This study examines the effect of cinnamic (Cin) and caffeic (Caf) acids, which are also phenolic precursors, on stilbene biosynthesis in the cell cultures. Five stilbenes, t-resveratrol diglucoside, t-piceid (t-resveratrol glucoside), t-resveratrol, t-ε- and t-δ-viniferins, were found in the treated and untreated cells. Cin increased the total stilbene production in the grape cell cultures 2...
March 22, 2017: Biotechnology and Applied Biochemistry
Marzieh Jafari, Mohsen Rezaei, Heibatullah Kalantari, Maryam Tabarzad, Bahram Daraei
DNAzyme and aptamer conjugations have already been used for sensitive and accurate detection of several molecules. In this study, we tested the relationship between conjugation orientation of DNAzyme and Aflatoxin B1 aptamer and their subsequent peroxidase activity. Circular dichroism (CD) spectroscopy and biochemical analysis were used here to difference between these two conjugation patterns. Results showed that DNAzyme-aptamer has more catalytic activity and efficiency than aptamer-DNAzyme. Thereby, DNAzyme-aptamer with its superior efficiency can be used for design and development of more sensitive Aflatoxin B1 DNA based biosensors...
March 22, 2017: Biotechnology and Applied Biochemistry
Agata Wasak, Radosław Drozd, Łukasz Struk, Bartłomiej Grygorcewicz
The aim of this study was to investigate the optimal conditions for the immobilisation and stabilisation of DyP1B Dye-decolourising peroxidases type B (DyP1B) from Pseudomonas fluorescens Pf-5 immobilised in Ca-alginate ferromagnetic beads (Ca-alginate FeBs). The immobilised DyP1B was used in the degradation of the Reactive Blue 5 synthetic dye. The enzyme was successfully entrapped in a Ca-alginate matrix, and showed an encapsulation efficiency of 94%. The concentration of 0.8 mg mL(-1) DyP1B, 2% of alginate and 10 mg mL(-1) magnetite were optimal for immobilisation...
March 21, 2017: Biotechnology and Applied Biochemistry
Shalini Raik, Ajay Kumar, Shalmoli Bhattacharyya
Current advances in medicine have revolutionized the field of regenerative medicine dramatically with newly evolved therapies for repair or replacement of degenerating or injured tissues. Stem cells can be harvested from different sources for clinical therapeutics which include fetal tissues, umbilical cord blood, embryos and adult tissues. Stem cells can be isolated and differentiated into desired lineages for tissue regeneration and cell replacement therapy. However, several loopholes need to be addressed properly before this can be extended for large scale therapeutic application...
March 21, 2017: Biotechnology and Applied Biochemistry
Wei Jiang, Beibei Lv, Xiao Wu, Jinbin Wang, Guogan Wu, Chunhui Shi, Xueming Tang
In this study, trends in synonymous codons usage of Volvariella volvecea have been first examined by analysis of complete coding sequences and gene chip data. The results showed that GC content at three codon positions are obviously different and there were several factors shaping the codon usage of V. volvacea genes, including base composition. The comparison of codon usage among four edible fungi such as V. volvacea, Agaricus bisporus, Coprinopsis cinerea, and Pleurotus ostreatus indicated that the similar codon usage pattern was used among V...
March 2017: Biotechnology and Applied Biochemistry
Juan Wang, Jing Li, Xiaolei Wu, Shujie Liu, Hongfa Li, Wenyuan Gao
Glycyrrhiza uralensis has acquired significant importance due to its medicinal properties and health function. In this study, the quality of G. uralensis adventitious roots was evaluated in terms of genetic stability, active compounds, and anti-inflammatory activity. Monomorphic banding pattern obtained from the mother plant and tissue cultures of G. uralensis with randomly amplified polymorphic DNA markers confirmed the genetic stability of adventitious roots. Neoliquiritin (neoisoliquiritin), ononin, liquiritin, and glycyrrhizic acid were identified from G...
March 2017: Biotechnology and Applied Biochemistry
Hong-De Yan, Zhao Wang, Jun-Qing Qian
A mycelium-bound lipase from Aspergillus oryzae (AOL) exhibited excellent enantioselectivity for kinetic resolution of (RS)-1-phenylethanol ((RS)-1-PE) in organic solvent. The various reaction parameters affecting the conversion and enantioselectivity were studied, including type of acyl donor, solvent, molar ratio, temperature, enzyme amount, and substrate concentration. The optimum reaction conditions were found to be transesterification with vinyl acetate at 30 °C in methyl tert-butyl ether with a vinyl acetate: (RS)-1-PE molar ratio of 1:1 and an enzyme concentration of 60 g/L...
March 2017: Biotechnology and Applied Biochemistry
Mohd Rafais Mohd Razaif-Mazinah, Siti Nor Syairah Anis, Hazwani Izzati Harun, Khairunnisa Abdul Rashid, Mohamad Suffian Mohamad Annuar
Pseudomonas putida Bet001 and Delftia tsuruhatensis Bet002, isolated from palm oil mill effluent, accumulated poly(3-hydroxyalkanoates) (PHAs) when grown on aliphatic fatty acids, sugars, and glycerol. The substrates were supplied at 20:1 C/N mole ratio. Among C-even n-alkanoic acids, myristic acid gave the highest PHA content 26 and 28 wt% in P. putida and D. tsuruhatensis, respectively. Among C-odd n-alkanoic acids, undecanoic gave the highest PHA content at 40 wt% in P. putida and 46 wt% in D. tsuruhatensis on pentadecanoic acid...
March 2017: Biotechnology and Applied Biochemistry
Neyssene Aissaoui, Ferid Abidi, Julie Hardouin, Zaineb Abdelkafi, Naziha Marrakchi, Thierry Jouenne, M Nejib Marzouki
Fish protein hydrolysate was prepared from muscle of small red scorpionfish (Scorpaena notata) by treatment with a protease from the fungus Penicillium digitatum. Protein hydrolysate was found to strongly inhibit the angiotensin I converting enzyme and exhibited high antioxidative activity through 1,1-diphenyl-2-picrylhydrazyl free radical scavenging assay. After ultrafiltration, peptides were isolated by a two-step procedure: size exclusion chromatography on a Toyopearl HW-40 followed by reversed-phase high-performance liquid chromatography with a high purification yield of 2...
March 2017: Biotechnology and Applied Biochemistry
Konanani Rashamuse, Walter Sanyika Tendai, Kgama Mathiba, Thobile Ngcobo, Sibongile Mtimka, Dean Brady
In recent years, there have been particular emphases worldwide on the development and optimization of bioprocesses for the utilization of biomass. An essential component of the biomass processing conduit has been the need for robust biocatalysts as high-performance tools for both the depolymerization of lignocellulosic biomass and synthesis of new high-value bio-based chemical entities. Through functional screening of the metagenome of the hindgut bacterial symbionts of a termite, Trinervitermes trinervoides, we discovered open reading frames for 25 cellulases and hemicellulases...
March 2017: Biotechnology and Applied Biochemistry
Huiqun Wang, Chenchen Guo, Bo-Zhen Chen, Mingjuan Ji
NS3 protease plays a vital role in the replication of the hepatitis C virus (HCV). BMS-605339 is a novel linear tetra-peptide α-ketoamide inhibitor of NS3 protease and shows specificity for HCV NS3 protease genotype 1a and genotype 1b. Mutation at the key site 168 of the HCV NS3 protease can induce resistance to BMS-605339, which greatly affects the antiviral therapy efficacy to hepatitis C. In the present study, we employed molecular dynamics simulations, free energy calculations, and free energy decomposition to explore the drug resistance mechanism of BMS-605339 due to the three representative mutations D168C/Y/V...
March 2017: Biotechnology and Applied Biochemistry
Jinxin Li, Juan Wang, Xiaolei Wu, Dahui Liu, Jing Li, Jianli Li, Shujie Liu, Wenyuan Gao
Panax notoginseng, an important herbal medicine, has wide uses for its bioactive compounds and health function. In this work, we compared the content of saponin in cultivation and adventitious root. The total content of saponins in adventitious root (8.48 mg⋅g(-1) ) was found lower than in the native one (3-year-old) (34.34 mg⋅g(-1) ). To enhance the content of bioactive compounds, we applied elicitors jasmonic acid (JA) and methyl dihydrojasmonate (MDJ) to the adventitious root culture. It was observed that the highest total content of saponins (71...
March 2017: Biotechnology and Applied Biochemistry
Xinyu Zhang, Jiang Yu, Aiwu Zeng
In this paper, cotton seed oil deodorizer distillate (CSODD), was recovered to obtain fatty acid sterol ester (FASE), which is one of the biological activated substances added as human therapeutic to lower cholesterol. Esterification reactions were carried out using Candida rugosa lipase as a catalyst, and the conversion of phytosterol was optimized using response surface methodology. The highest conversion (90.8 ± 0.4%) was reached at 0.84 wt% enzyme load, 1:25 solvent/CSODD mass ratio, and 44.2 °C after 12 H reaction...
March 2017: Biotechnology and Applied Biochemistry
Dong Hu, Hong Yang, Jiangtao Liu, Zhongli Lei
A novel type of pH-responsive star polymer based on β-cyclodextrin (β-CD) was synthesized and further covalently conjugated with enzyme. The impact of its self-assembly behavior on enzyme activity was investigated. In our design, azide containing the polymer (N3 )7 -β-CD-(PtBA)14 was synthesized via atom transfer radical polymerization of tert-butyl acrylate using (N3 )7 -β-CD-(Br)14 as the multifunctional initiator. The final product (N3 )7 -β-CD-(PAA)14 was obtained via hydrolysis and covalently conjugating pectinase onto pH-responsive polyacrylic acid (PAA) arms...
March 2017: Biotechnology and Applied Biochemistry
Zhen-Tao Song, Ming-Jun Zhu
Fermentation of herb Polygonum hydropiper L. (PHL) and cassava pulp (CP) for feed additive production with simultaneous flavonoid dissolution was investigated, and a two-stage response surface methodology (RSM) based on Plackett-Burman factorial design (PB design) was used to optimize the flavonoid dissolution and protein content. Using the screening function of PB design, four different significant factors for the two response variables were acquired: factors A (CP) and B (PHL) for the flavonoid dissolution versus factors G (inoculum size) and H (fermentation time) for protein content...
March 2017: Biotechnology and Applied Biochemistry
Xuedong Wang, Hui Long, Danhong Shen, Long Liu
The sialidase gene from Brevibacterium casei was cloned in pET28a and overexpressed as a histidine-tagged protein in Escherichia coli BL21(DE3). The histidine-tagged sialidase protein was purified and characterized from the crude cell extracts of isopropyl-β-d-thiogalactopyranoside-induced cells using Ni-NTA agarose chromatography. SDS-PAGE using the purified sialidase indicated a single band at 116 kDa. This sialidase showed maximum activity at a pH of 5.5 and temperature of 37 °C. The kinetic parameters Km and Vmax for the artificial substrate 2'-(4-methylumbelliferyl)-α-d-N-acetyl-neuraminic acid sodium salt hydrate were 1...
March 2017: Biotechnology and Applied Biochemistry
Subbi Rami Reddy Tadi, Arun E V R, Anil Mukund Limaye, Senthilkumar Sivaprakasam
The aim of this study is to optimize the production of optically pure d (-) lactic acid (DLA) employing a cost-effective production medium. Based on the designed biomass approach, Sporolactobacillus inulinus NBRC 13595 was found to exhibit high DLA titer (19.0 g L(-1) ) and optical purity (99.6%). A cost-effective medium was constituted using Palmyra palm jaggery (PJ) from Borassus flabellifer and whey protein hydrolysate (WPH) as carbon and nitrogen sources, respectively. Plackett-Burman design indicated that PJ, WPH, and MnSO4 as significant variables influence DLA production...
March 2017: Biotechnology and Applied Biochemistry
Keke Shao, Xinhui Shi, Xiangjun Zhu, Leilei Cui, Qixiang Shao, Da Ma
Construction of a random ssDNA sublibrary is an important step of the aptamer screening process. The available construction methods include asymmetric PCR, biotin-streptavidin separation, and lambda exonuclease digestions, in which PCR amplification is a key step. The main drawback of PCR amplification is overamplification increasing nonspecific hybridization among different products and by-products, which may cause the loss of potential high-quality aptamers, inefficient screening, and even screening failure...
March 2017: Biotechnology and Applied Biochemistry
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