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Histology and Histopathology

Saibin Wang, Qian Ye, Junwei Tu
Stroke-associated ocular disorders are vision-threatening. This study was designed to evaluate in vivo retinal injury induced by transient global cerebral ischemia/reperfusion (I/R). A stroke-induced retinal injury model in Wistar rats was established by electrocoagulation of bilateral vertebral arteries, combined with transient ligation of the bilateral common carotid arteries. Rats were randomly divided into groups based on the time post cerebral perfusion (3 h, 24 h, 48 h, 72 h, and 7 days). Retinal injury was evaluated by histological analysis, examination of eye fundus, and TUNEL staining...
November 9, 2018: Histology and Histopathology
Adriana Palmeira Dias Rodrigues, Anelize Sartori Santos Bortolozzo, Fernanda Magalhães Arantes-Costa, Beatriz Mangueira Saraiva-Romanholo, Flávia Castro Ribas de Souza, Thayse Regina Brüggemann, Fernanda Paula Roncon Santana, Marlon Vilela de Brito, Camila Ramalho Bonturi, Natalia Neto Dos Santos Nunes, Carla Máximo Prado, Edna Aparecida Leick, Maria Luiza Vilela Oliva, Milton de Arruda Martins, Renato Fraga Righetti, Iolanda de Fátima Lopes Calvo Tibério
INTRODUCTION: Proteinase inhibitors have been associated with anti-inflammatory and antioxidant activities and may represent a potential therapeutic treatment for asthma. PURPOSE: The aim of the present study was to evaluate the effects of Enterolobium contortisiliquum trypsin inhibitor (EcTI) on pulmonary mechanical function, eosinophilic recruitment, inflammatory cytokines, remodeling and oxidative stress in an experimental model of chronic allergic pulmonary inflammation...
November 8, 2018: Histology and Histopathology
Carolin Matschurat, Kristina Rode, Julia Hollenbach, Karola Wolf, Carola Urhausen, Andreas Beineke, Anne-Rose Günzel-Apel, Ralph Brehm
Impairment of blood-testis barrier integrity can be observed during inflammation, infection, trauma and experimental autoimmune orchitis, which is inducible in rodents. In the present study, an initially fertile two-year-old Beagle dog was presented with a decline in total sperm number resulting in azoospermia within five months, verified by twice-monthly semen analyses. The dog was clinically healthy with bilateral small testes and showed normal thyroid function. Bacterial cultures of semen were negative and serum biochemical analyses showed no abnormal findings...
November 7, 2018: Histology and Histopathology
Irina Arutyunyan, Timur Fatkhudinov, Andrey Elchaninov, Olesya Vasyukova, Andrey Makarov, Natalia Usman, Evgeniya Kananykhina, Anastasiya Lokhonina, Dmitry Goldshtein, Galina Bolshakova, Gennady Sukhikh
This study delves into possible mechanisms underlying the stimulating influence of UC-MSCs transplantation on functional and structural recovery of ischemic skeletal muscles. Limb ischemia was created in Sprague-Dawley rats by excision of femoral and popliteal arteries. Allogeneic rat PKH26-labeled UC-MSCs were administered by direct intramuscular injection. Animals of experimental group responded to the transplantation by improvement in their locomotor function as assessed by the rotarod performance test on day 9 and 29 after transplantation...
November 6, 2018: Histology and Histopathology
Tülin Firat, Gizem Söyler, Fatma Töre, Mustafa Şit, Aysu Kiyan, Ufuk Özgen, Aysel Kükner
Following an injury or resection, the mammalian liver has the capacity to regain its former volume and functioning by restoring itself. Studies have demonstrated that antioxidants play a role in hepatic regeneration. This study investigated the effect of 4-(3,4-dihydroxybenzoyloxymethyl) phenyl-O-β-D-glucopyranoside (PG) obtained from Origanum micranthum on liver regeneration. Sixty Wistar Albino rats were used. In the sham-operated group, a midline abdominal laparotomy was performed without hepatectomy. In the partial hepatectomy (PHx) group, the median and left lateral lobes were removed...
November 5, 2018: Histology and Histopathology
Zhonghou Wang, Yuta Yoshida, Naomi E Kramer, Fuminori Kawabata, Shoji Tabata, Woo K Kim, Hong-Xiang Liu
Like other epithelial cells, taste bud cells have a short life span and undergo continuous turnover. An active stem or progenitor cell niche is essential for taste bud formation and maintenance. Early taste bud cells have a life span of ~4 days on average in chicken hatchlings when taste buds grow rapidly and undergo maturation. The average life span is shorter than that of mature taste bud cells of rodents (~10-12 days on average). To better understand the mechanism underlying taste bud growth and homeostasis in chickens, we analyzed the distribution of proliferating cells in different tissue compartments, including taste buds, the surrounding epithelium and the underlying connective tissue in P1-3 hatchlings and P45 chickens...
October 31, 2018: Histology and Histopathology
Friederike Gaertner, Sandra Krüger, Christian Röder, Anna Trauzold, Christoph Röcken, Holger Kalthoff
The expression of five members of the TNF receptor superfamily and two of their ligands in human pancreatic ductal adenocarcinoma were investigated in parallel by immunohistochemistry. 41 patients with histologically confirmed ductal carcinoma of the pancreas were enrolled in this study in order (i) to compare the individual TNFR-SF expression and their ligands in PDAC-cells and (ii) to investigate their correlation with survival data. All patients had undergone pancreaticoduodenectomy and were staged as pT3N1M0...
October 24, 2018: Histology and Histopathology
Andreia Albuquerque, Elisabete Rios, Rui Medeiros
Histological grading of squamous intraepithelial lesions or intraepithelial neoplasia is fundamental for clinical management and for assessment of the risk of progression. Biomarkers are important for assisting correct grading of these lesions, reducing inter and intraobserver variability and most promising, for prognosis. Although p16 is the most studied biomarker in this setting, there are several other biomarkers that have been studied, reflecting also the need to find a better single or association option that can be more suitable, especially for classification purposes...
October 19, 2018: Histology and Histopathology
Siripa Rodniem, Vilailak Tiyao, Cheng Nilbu-Nga, Raksawan Poonkhum, Sirinun Pongmayteegul, Wisuit Pradidarcheep
Liver fibrosis is an excessive accumulation of scar tissue resulting from inflammation and cell death. Thioacetamide (TAA) is a well-known hepatotoxin that induces liver fibrosis. A marker of injured hepatocytes is transforming growth factor-beta 1 (TGF-β1), while alpha-smooth muscle actin (α-SMA) and tissue inhibitor of metalloproteinase 1 (TIMP-1) are markers of activated hepatic stellate cells. Alpha-mangostin, a major xanthone derivative from the mangosteen pericarp, has been shown to have anti-oxidant and anti-inflammatory activities...
October 11, 2018: Histology and Histopathology
Rong Yin, Shawn J Rice, Jinwei Wang, Lin Gao, Joseph Tsai, Radean T Anvari, Fang Zhou, Xin Liu, Gang Wang, Yuxin Tang, Martin C Mihm, Chandra P Belani, Dong-Bao Chen, J Stuart Nelson, Wenbin Tan
INTRODUCTION: Port wine stain (PWS) is characterized as a progressive dilatation of immature venule-like vasculatures which result from differentiation-impaired endothelial cells. In this study, we aimed to identify the major biological pathways accounting for the pathogenesis of PWS. METHODS: Sequential windowed acquisition of all theoretical fragment ion mass spectra (SWATH-MS) was used to identify differentially expressed proteins in PWS lesions, followed by confirmative studies with immunohistochemistry, immunoblot and transmission electron microscopy (TEM)...
October 10, 2018: Histology and Histopathology
Ulrike Rüder, Carsten Denkert, Catarina Alisa Kunze, Paul Jank, Judith Lindner, Korinna Jöhrens, Hagen Kulbe, Jalid Sehouli, Manfred Dietel, Elena Braicu, Silvia Darb-Esfahani
APOBEC3 enzymes are part of the innate immune system and they are important in retroviral defense. The number of mutations in ovarian cancer increases with rising levels of APOBEC3B mRNA. We could confirm that APOBEC3B mRNA is upregulated in ovarian cancer cell lines and in ovarian cancer tissue. We evaluated APOBEC3B expression in histologically defined subtypes of ovarian cancer to identify its influence on overall survival (OS) and progression-free survival (PFS). Tissue microarrays from 219 patients with high-grade serous (HGSC), 61 with low-grade serous (LGSC), 62 with endometrioid (EC) and 55 with clear cell (CCC) ovarian carcinoma were stained using an antibody against APOBEC3B...
October 5, 2018: Histology and Histopathology
Veronika Šimaiová, Viera Almášiová, Katarína Holovská, Terézia Kisková, Františka Horváthová, Zuzana Ševčíková, Štefan Tóth, Adam Raček, Enikő Račeková, Katarína Beňová, Petr Dvořák, Viera Cigánková
BACKGROUND: Nowadays, mobile devices that emit non-ionizing electromagnetic radiation (EMR) are predominantly used by juveniles and pubescents. The aim of the present study was to evaluate the effect of whole body pulsed EMR on the juvenile Wistar albino rat testis at a frequency of 2.45 GHz and mean power density of 2.8 mW/cm². METHODS: The investigated animals (n=24) were divided into two control and two EMR groups (5 and 6 week old rats; 6 rats per group). Both EMR groups were irradiated continually for 3 weeks (2h/day) from postnatal days 14 and 21, respectively...
September 27, 2018: Histology and Histopathology
Wei Qian, Zilong Wang, Tongda Xu, Dongye Li
Prompt myocardial reperfusion during acute myocardial infarction by fibrinolytic therapy, percutaneous coronary intervention, or coronary artery bypass grafting limits the affected area and improves prognosis. However, reperfusion itself can cause cardiomyocyte damage and decrease treatment efficacy. No treatments that effectively prevent myocardial ischemia/reperfusion (I/R) injury are currently available, and are therefore the focus of ongoing research. Salvianolic acid A (SAA), the active ingredient of the traditional Chinese herbal remedy Salvia miltiorrhiza, has anti-thrombotic activity, anti-inflammatory, and anti-cancer activity; regulates blood lipids and provides hepatic and neural protection...
September 26, 2018: Histology and Histopathology
Caroline Simão Sarti, Renata Schlesner de Oliveira, Priscila Hernández de Campos, Tatiane Fernandes Novaes, Michele Baffi Diniz, Jonas de Almeida Rodrigues
The aims of this study were to evaluate the influence (1) of the examiner experience and (2) three histological classification criteria on the reproducibility of two gold standard techniques (non-dye or dye) for validation of the diagnosis of occlusal caries. This study comprised a sample of 210 digital images of 105 permanent teeth (105 rhodamine B dyed and 105 dye-free hemisections) and six examiners. Images were evaluated on a laptop computer and categorised according to three different histological classification criteria (proposed by Ekstrand, Lussi or Downer) and repeated in order to allow reproducibility calculation...
September 25, 2018: Histology and Histopathology
Pallavi A Patil, Jesse K McKenney, Jordan P Reynolds, Christopher G Przybycin, Cristina Magi-Galluzzi
BACKGROUND: Although ERG and SPINK1 molecular alterations have been studied in acinar and ductal adenocarcinoma of the prostate, EZH2 expression has not been previously evaluated in ductal adenocarcinoma. METHODS: We collected cases of pure and mixed ductal adenocarcinoma of the prostate and evaluated clinical significance and EZH2, ERG, and SPINK1 protein expression. RESULTS: We investigated 61 ductal adenocarcinomas, 22 pure and 39 mixed ductal/acinar...
September 24, 2018: Histology and Histopathology
Hanrui Chen, Man Shu, Sile Chen, Ling Xue, Yuan Lin
AIMS: Mixed adenoneuroendocrine carcinoma (MANEC), also known as high- grade mixed neuroendocrine-non-neuroendocrine neoplasm (MiNEN) in the World Health Organization (WHO) classification of tumors of the endocrine organs (2017), is a rare gastric malignancy. Here, we present 10 cases of gastric MANEC and analyse their clinicopathological features and lymph node metastatic patterns. METHODS AND RESULTS: Six patients were male, and four were female. The mean age of the patients was 67...
September 21, 2018: Histology and Histopathology
Yu Feng, Zhe Cai, Wing Ki Cheung, Kedi Yang, Lei Xu, William Weijia Lu, Haibo Yang, Kwong-Yuen Chiu
Various histological staining methods have been explored to detect the joint lesions in osteoarthritis (OA), but these histological stains cannot comprehensively present the comparatively complex structures of articular cartilage in knee OA. In addition, no integrated histological staining method can be used to evaluate efficiently both the subzone region and matrix composition in cartilage containing tissues. Therefore, in this study, a novel multichromatic staining method termed TTF staining, using Toluidine Blue (T), Tartrazine (T) and Fast Green (F) sequential combined staining for histological analysis, has been exploited to characterize the changes of matrix components and contents in cartilage during OA and in the bone development...
September 20, 2018: Histology and Histopathology
Yiqi Rui, Mingchao Hu, Peng Wang, Chuanqiang Zhang, Hua Xu, Yuanzhong Li, Yu Zhang, Jianchun Gu, Qiang Wang
Recent studies highlight long non-coding RNAs (lncRNAs) as key regulators of cancer biology that contribute to carcinogenesis. The lncRNA HOXA transcript at the distal tip (HOTTIP) is involved in the development of several cancers. Previous studies demonstrated that HOTTIP could promote colorectal cancer (CRC) cell proliferation via silencing of p21 expression. However, the potential role of HOTTIP in CRC metastasis has not yet been discussed. Here, we found that HOTTIP level was significantly higher in CRC than in corresponding adjacent normal tissues, and patients with a larger tumor size, advanced pathological stage, or distant metastasis had higher HOTTIP expression...
September 19, 2018: Histology and Histopathology
L P Bialy, U Kuckelkorn, P Henklein, J Fayet, G M Wilczyński, A Kamiński, I Mlynarczuk-Bialy
Extralysosomal proteolysis is a multistep process involving the Ubiquitin- Proteasome System (UPS) and supplementary peptidases. Tripeptidyl peptidase II (TPPII) is the most extensively characterized enzyme, supplementing and sometimes substituting for proteasomal functions. In response to proteasome inhibition, polyubiquitinated proteins acting as proteasome substrates aggregate with proteasomes and form aggresomes. Several proteasome inhibitors are used as anti-cancer drugs. Thus, in our study, we used a novel fluorescent-tagged proteasome inhibitor BSc2118 to induce aggresome formation in C26 murine colon adenocarcinoma cells...
September 18, 2018: Histology and Histopathology
Long-Lei Cao, Jie Yu, Zhang-Ling Yang, Xu Qiao, Hui Ye, Chang-Lei Xi, Qi-Chang Zhou, Cheng-Cheng Hu, Chao-Jun Zhao, Zhi-Lin Gong
OBJECTIVE: To explore the MMP-1/TIMP-1 expressions in rectal submucosa of females with obstructed defecation syndrome (ODS) associated with internal rectal prolapse (IRP). METHODS: Fifty-six female patients with ODS associated with IRP were enrolled as Case group, and 43 female hemorrhoids of stages III-IV without constipation and IRP were enrolled as Control group. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and immunohistochemistry were performed to test the expressions of MMP-1/TIMP-1 in the rectal submucosa...
September 17, 2018: Histology and Histopathology
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