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Histology and Histopathology

Hanrui Chen, Man Shu, Sile Chen, Ling Xue, Yuan Lin
AIMS: Mixed adenoneuroendocrine carcinoma (MANEC), also known as high- grade mixed neuroendocrine-non-neuroendocrine neoplasm (MiNEN) in the World Health Organization (WHO) classification of tumors of the endocrine organs (2017), is a rare gastric malignancy. Here, we present 10 cases of gastric MANEC and analyse their clinicopathological features and lymph node metastatic patterns. METHODS AND RESULTS: Six patients were male, and four were female. The mean age of the patients was 67...
September 21, 2018: Histology and Histopathology
Yu Feng, Zhe Cai, Wing Ki Cheung, Kedi Yang, Lei Xu, William Weijia Lu, Haibo Yang, Kwong-Yuen Chiu
Various histological staining methods have been explored to detect the joint lesions in osteoarthritis (OA), but these histological stains cannot comprehensively present the comparatively complex structures of articular cartilage in knee OA. In addition, no integrated histological staining method can be used to evaluate efficiently both the subzone region and matrix composition in cartilage containing tissues. Therefore, in this study, a novel multichromatic staining method termed TTF staining, using Toluidine Blue (T), Tartrazine (T) and Fast Green (F) sequential combined staining for histological analysis, has been exploited to characterize the changes of matrix components and contents in cartilage during OA and in the bone development...
September 20, 2018: Histology and Histopathology
Yiqi Rui, Mingchao Hu, Peng Wang, Chuanqiang Zhang, Hua Xu, Yuanzhong Li, Yu Zhang, Jianchun Gu, Qiang Wang
Recent studies highlight long non-coding RNAs (lncRNAs) as key regulators of cancer biology that contribute to carcinogenesis. The lncRNA HOXA transcript at the distal tip (HOTTIP) is involved in the development of several cancers. Previous studies demonstrated that HOTTIP could promote colorectal cancer (CRC) cell proliferation via silencing of p21 expression. However, the potential role of HOTTIP in CRC metastasis has not yet been discussed. Here, we found that HOTTIP level was significantly higher in CRC than in corresponding adjacent normal tissues, and patients with a larger tumor size, advanced pathological stage, or distant metastasis had higher HOTTIP expression...
September 19, 2018: Histology and Histopathology
L P Bialy, U Kuckelkorn, P Henklein, J Fayet, G M Wilczyński, A Kamiński, I Mlynarczuk-Bialy
Extralysosomal proteolysis is a multistep process involving the Ubiquitin- Proteasome System (UPS) and supplementary peptidases. Tripeptidyl peptidase II (TPPII) is the most extensively characterized enzyme, supplementing and sometimes substituting for proteasomal functions. In response to proteasome inhibition, polyubiquitinated proteins acting as proteasome substrates aggregate with proteasomes and form aggresomes. Several proteasome inhibitors are used as anti-cancer drugs. Thus, in our study, we used a novel fluorescent-tagged proteasome inhibitor BSc2118 to induce aggresome formation in C26 murine colon adenocarcinoma cells...
September 18, 2018: Histology and Histopathology
Long-Lei Cao, Jie Yu, Zhang-Ling Yang, Xu Qiao, Hui Ye, Chang-Lei Xi, Qi-Chang Zhou, Cheng-Cheng Hu, Chao-Jun Zhao, Zhi-Lin Gong
OBJECTIVE: To explore the MMP-1/TIMP-1 expressions in rectal submucosa of females with obstructed defecation syndrome (ODS) associated with internal rectal prolapse (IRP). METHODS: Fifty-six female patients with ODS associated with IRP were enrolled as Case group, and 43 female hemorrhoids of stages III-IV without constipation and IRP were enrolled as Control group. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and immunohistochemistry were performed to test the expressions of MMP-1/TIMP-1 in the rectal submucosa...
September 17, 2018: Histology and Histopathology
Shuwei Wang, Yutong Yin, Shuang Liu
Glioma is the most common and aggressive type of brain tumor. It has a poor prognosis and a high recurrence rate. Despite continued advances in surgery, chemotherapy and radiotherapy, the clinical outcomes remain dismal. MicroRNAs (miRNAs) play important roles in the initiation and progression of a multitude of tumors. Until now, the molecular mechanism that is responsible for glioma tumorigenesis and progression remains unclear. Increasing evidence has shown that miRNAs play an important role in glioma. In this review, we focus on the current advances in determining the role of miRNAs in regulating tumorigenesis and the progression of glioma...
September 14, 2018: Histology and Histopathology
Tingyi Sun, Yaxi Wang, Shilong Hu, Haimei Sun, Shu Yang, Bo Wu, Fengqing Ji, Deshan Zhou
The alteration of intestinal mucosal barrier is considered to be the central pathophysiological process in response to gastrointestinal infections, and mucosal microstructural damage is a major factor for enhancing epithelial permeability in persistent bacterial infections. However, the mechanism involved in hyperpermeability in the early stage of acute bacterial infections is not fully understood. In the present study, fluorescein isothiocyanate-dextran across and transepithelial resistance measured in Ussing chambers were used to assess the intestinal paracellular permeability...
September 13, 2018: Histology and Histopathology
Camila Couto Figueiredo, Núbia Braga Pereira, Luciana Xavier Pereira, Laser Antônio Machado Oliveira, Paula Peixoto Campos, Silvia Passos Andrade, Luciana Moro
A crucial component of the integration between foreign implants and the host is angiogenesis. However, to date, none of the available techniques and/or endothelial markers employed to assess angiogenesis in the implant/host interface seems to be able to highlight vascular structures convincingly. In the present study we investigated and compared the expression of two endothelial cell markers: platelet endothelial cell adhesion molecule (PECAM-1) (CD31) and endoglin (CD105) using immunohistochemistry (IHC) and immunofluorescence (IF) to identify and quantify newly formed blood vessels in subcutaneous implants of polyether-polyurethane sponge of formalin-fixed paraffin-embedded tissue...
September 12, 2018: Histology and Histopathology
Yoon Jin Cha, Haerin Jang, Ja Seung Koo
PURPOSE: We evaluated the expression of glutaminolysis-related proteins in Hurthle cell neoplasms (HCN) and follicular neoplasms (FN) of the thyroid, and investigated its clinical implication. METHODS: Tissue microarrays were constructed from 264 FNs (112 follicular carcinomas [FCs] and 152 follicular adenomas [FAs]) and 108 HCNs (27 Hurthle cell carcinomas [HCCs] and 81 Hurthle cell adenomas [HCAs]. The immunohistochemical staining result of 3 glutaminolysis-related proteins (Glutaminase 1 [GLS1], glutaminate dehydrogenase [GDH] and alanine- serine, cysteine-preferring transporter 2 [ASCT2]) was analyzed...
September 7, 2018: Histology and Histopathology
Saeid Shahrabi, Elham Homaei Hadad, Ali Amin Asnafi, Masumeh Maleki Behzad, Ali Ehsanpour, Najmaldin Saki
Human leukocyte antigens (HLA), which are a group of antigen-presenting proteins, are classified into two main groups: classic (including HLA-I, HLA-II, HLA-III) and non-classic. These molecules are expressed on the surface of several immune cells, which contribute to the defense of body against foreign antigens. Changing expressions of these molecules on tumor cells can be related to reduced ability of the immune system in killing tumor cells, as well as metastasis induction of many solid tumors. The purpose of this review article is to assess the possible relationship between changing expressions of HLA molecules with cancer metastasis and relapse...
September 6, 2018: Histology and Histopathology
Adolfo Soto-Domínguez, Humberto Rodríguez-Rocha, Aracely García-García, Odila Saucedo-Cárdenas, Luis E Rodríguez-Tovar, Uziel Castillo-Velázquez
The biological behavior of teratomas depends on several interdependent clinical and epidemiological variables such as age at diagnosis, sex, tumor microenvironment, and tumor morphology, among others. All these variables are correlated to different cytogenetic and molecular aberrations (Harms et al, 2006). There are null reports of teratomas in pigs. The aim of this study was to characterize the tissues present in a mature congenital intraneural teratoma in the cerebellum area of a Landrace female pig of 6-7 weeks old...
September 5, 2018: Histology and Histopathology
Lukáš Lacina, Jan Brábek, Vladimír Král, Ondřej Kodet, Karel Smetana
Interleukin-6 is a multifaceted cytokine, usually reported as a pro-inflammatory molecule. However, certain anti-inflammatory activities were also attributed to IL-6. The levels of IL-6 in serum as well as in other biological fluids are elevated in an age-dependent manner. Notably, it is consistently reported also as a key feature of the senescence-associated secretory phenotype. In the elderly, this cytokine participates in the initiation of catabolism resulting in, e.g. sarcopenia. It can cross the blood-brain barrier, and so it is in causal association with, e...
September 4, 2018: Histology and Histopathology
Guang-Da Yang, Zhi-Chao Wang, Qian-Ya Chen, Hai-Liang Zhang, Xian-Gan Lin, Tie-Jun Huang, Chao-Nan Qian, Bi-Jun Huang
The controversy of (p53) in nasopharyngeal carcinoma persists, despite the fact that many studies have been conducted on its correlation with Latent membrane protein 1 (LMP1), bcl-2, and prognosis. To better understand this postulated relationship, a meta-analysis was performed based on existing relevant studies. A total of 19 individual studies with a total of 1189 patients were included in the meta-analysis. Overall, the results revealed a significant association of p53-positive status with a poor 5-year survival of nasopharyngeal carcinoma (NPC) patients as the risk difference (RD) was -0...
July 31, 2018: Histology and Histopathology
Robert Terry, JayaKrishna Chintanaboina, Deep Patel, Brittany Lippert, Matthew Haner, Kimberly Price, Amanda Tracy, Alexander Lalos, Michelle Wakeley, Linda S Gutierrez
The WNT/β-catenin cellular network has been extensively studied in numerous diseases including inflammatory bowel disease (IBD). IBD is a condition that increases the risk of developing colorectal cancer. WIF-1 is an inhibitory protein that acts by blocking the interactions of WNT with its receptor complex, thus leading to downregulation of end products of this pathway. While WIF-1 has been characterized in several cancers, its relationship with IBD has yet to be elucidated. In this study, the expression of WIF-1 in patients with IBD was analyzed in order to provide insights into the pathophysiology and rationale for alternative therapies...
July 27, 2018: Histology and Histopathology
Yufeng Huang, Yan Cui, Sijiu Yu, Junfeng He, Yanyu He, Qian Zhang, Penggang Liu, Yanhuan Pu, Juan Sun, Xinhua Kang
This experiment compared the histological characteristics and distribution of CD3 and CD79a among yak lymph nodes, hemal nodes and spleen. The three organs from ten clinically healthy newborn and adult yaks were studied using histology and immunohistochemistry. The yak hemal nodes, which consisted of blood sinuses, lymphoid follicles, diffuse lymphoid tissue and lymphoid cords, appeared to share the histological characteristics of the spleen and lymph nodes: the lymphoid follicles of the hemal nodes were much like those of the lymph nodes, which were not surrounded by the central artery and periarteriolar lymphoid sheath...
July 19, 2018: Histology and Histopathology
S Akhtar, G Petrovski, R Albert, A Alkanaan, O Kirat, A D Khan, T Almubrad
BACKGROUND: The transparency of the cornea is regulated by the unique organization of collagen fibrils (CFs) which is maintained by proteoglycans (PGs). The interlacing of CF lamellae in the anterior stroma provides the biomechanical properties of the cornea. OBJECTIVE: To investigate the alterations of CFs and PGs in the swollen cornea, with special reference to the anterior stroma by using electron microscopy and 3D ultrastructural tomography. METHOD: Nine healthy normal scleral corneal rings (age from 40 to 65 years) were hydrated individually in deionised water to induce swelling in the cornea...
July 18, 2018: Histology and Histopathology
Yoshiyuki Akiyama, Yukio Homma, Daichi Maeda
Interstitial cystitis/bladder pain syndrome (IC/BPS) is an umbrella term of chronic debilitating conditions of unknown etiology characterized by symptoms of lower urinary tract hypersensitivity such as bladder pain/discomfort, urgency, and urinary frequency. The pathological features of IC/BPS have been generally reported as non-specific chronic inflammatory changes, with mast cell infiltration as a potential key finding. However, growing evidence reveals a histological distinction between IC/BPS with Hunner lesions and IC/BPS without Hunner lesions, and also sheds doubt on the diagnostic value of the mast cell count...
July 17, 2018: Histology and Histopathology
Nicoletta F Maounis, Eduarda Dráberová, Nikos Trakas, Maria Chorti, Dimitra Riga, Kimon Tzannis, Meletis Kanakis, Kirtanaa Voralu, Eleni Ellina, Eleni Mahera, Maria Demonakou, Achilleas Lioulias, Pavel Dráber, Christos D Katsetos
INTRODUCTION: It has been reported that overexpression and altered compartmentalization of γ-tubulin may contribute to tumorigenesis and tumor aggressiveness in a variety of human malignancies. We have shown that γ-tubulin expression and cellular distribution pattern is also altered in non-small cell lung cancer (NSCLC) (Histol Histopathol. 2012; 27: 1183-1194). In the present study we examined the relationship between γ-tubulin expression and patient overall survival (OS). MATERIAL AND METHODS: Immunohistochemistry was performed, with well-characterized anti-γ-tubulin antibodies, on 109 formalin-fixed, paraffin-embedded NSCLC specimens (p-TNM stage I-III)...
July 16, 2018: Histology and Histopathology
Xuguang Liu, Huanwen Wu, Lianghong Teng, Hui Zhang, Junliang Lu, Zhiyong Liang
Encapsulated papillary carcinoma (EPC) of the breast is typically of low-to-intermediate grade (LTIG) with favorable prognosis. Rarely, high-grade (HG) EPC cases have been documented in recent years. Herein we compared the morphological, immunohistochemical, and clinical features of LTIG EPC to those of HG EPC. Of the 30 EPC patients, 25 were diagnosed as LTIG and five as HG (median age: 60 and 36 years, respectively); 80% of the HG EPCs exhibited predominantly solid architecture with prominent lymphoplasmacytic infiltrates, more crowded and thicker papillae, and greater stratification and irregular arrangement of malignant epithelial cells...
July 13, 2018: Histology and Histopathology
Paula R Giaretta, Jan S Suchodolski, Anna K Blick, Joerg M Steiner, Jonathan A Lidbury, Raquel R Rech
Takeda-G-protein-receptor-5 (TGR5) is a receptor for bile acids and its expression has been described in a variety of tissues and species. Characterization of TGR5 distribution and function has been investigated in drug discovery for the treatment of metabolic diseases in humans. Because dogs are one of the species used in biomedical research and share some similarities with human gastrointestinal diseases, the objective of this study was to characterize the distribution of TGR5 receptor in the canine species...
July 12, 2018: Histology and Histopathology
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