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Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire

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https://www.readbyqxmd.com/read/28197017/characterization-and-therapeutic-application-of-canine-adipose-mesenchymal-stem-cells-to-treat-elbow-osteoarthritis
#1
Éva Kriston-Pál, Ágnes Czibula, Zoltán Gyuris, Gyula Balka, Antal Seregi, Farkas Sükösd, Miklós Süth, Endre Kiss-Tóth, Lajos Haracska, Ferenc Uher, Éva Monostori
Visceral adipose tissue (AT) obtained from surgical waste during routine ovariectomies was used as a source for isolating canine mesenchymal stem cells (MSCs). As determined by cytofluorimetry, passage 2 cells expressed MSC markers CD44 and CD90 and were negative for lineage-specific markers CD34 and CD45. The cells differentiated toward osteogenic, adipogenic, and chondrogenic directions. With therapeutic aims, 30 dogs (39 joints) suffering from elbow dysplasia (ED) and osteoarthritis (OA) were intra-articularly transplanted with allogeneic MSCs suspended in 0...
January 2017: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/28154467/cleaning-with-a-wet-sterile-gauze-significantly-reduces-contamination-of-sutures-instruments-and-surgical-gloves-in-an-ex-vivo-pelvic-flexure-enterotomy-model-in-horses
#2
Gessica Giusto, Clara Tramuta, Vittorio Caramello, Francesco Comino, Patrizia Nebbia, Patrizia Robino, Ellen Singer, Elena Grego, Marco Gandini
The objective of this study was to investigate whether cleaning surgical materials used to close pelvic flexure enterotomies with a wet sterile gauze will reduce contamination and whether the use of a full thickness appositional suture pattern (F) or a partial thickness inverting (or Cushing) suture pattern (C) would make a difference in the level of contamination. Large colon specimens were assigned to group F or C and divided into subgroups N and G. In group G, a wet sterile gauze was passed over the suture material, another over the instruments, and another over the gloves...
January 2017: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/28154466/nasopharyngeal-temperature-measurement-in-sheep-during-general-anesthesia
#3
Tabita Tan, Jonathon Tuke, Gabrielle C Musk
The aim of this study was to compare nasopharyngeal and esophageal temperature measurements in anesthetized sheep with a range of fresh gas flows (1 to 6 L/min) through the breathing system. Data were compared using a Bland-Altman plot and correlation coefficients, and error measures were calculated. One hundred and ninety-five sets of data were collected from 20 sheep weighing 41 kg (31 to 51.5 kg). The bias (95% limit of agreement), correlation coefficient, and absolute error for nasopharyngeal compared to esophageal temperature were 0...
January 2017: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/28154465/escherichia-coli-isolated-from-feces-of-brown-bears-ursus-arctos-have-a-lower-prevalence-of-human-extraintestinal-pathogenic-e-coli-virulence-associated-genes
#4
Maruša Vadnov, Damjana Barbič, Darja Žgur-Bertok, Marjanca Starčič Erjavec
Eighty-six Escherichia coli strains from feces of either wild brown bears or those living in a zoo were screened for phylogenetic groups using the revisited Clermont phylotyping method and the prevalence of 24 virulence-associated genes (VAGs) of extraintestinal pathogenic E. coli (ExPEC). Our results showed that most strains of E. coli in bears belonged to phylogenetic groups III/IV/V (29%) and B1 (26%). Only half of the tested VAGs were found in the E. coli bear strains, with fimH present in 72%, ompT in 63%, and kpsMT in 43% of the strains...
January 2017: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/28154464/computed-tomographic-assessment-of-a-new-nonsurgical-sinus-trephination-technique-using-a-medical-bone-marrow-drill
#5
Victor Caudal, Elisabeth C Snead, Gregory S Starrak, Suresh Sathya, Cindy X Feng
The objective of this study was to determine the feasibility of trephination of the frontal sinus and injection of antifungal cream using a medical bone marrow drill in dogs. Results were compared with frontal sinus trephination using a standard surgical technique. Bilateral trephination of the frontal sinuses was carried out in the heads of 11 cadavers using a medical bone marrow drill and a surgical bone chuck. The time taken to carry out the procedure using both techniques was compared. Before and after injection of antifungal cream into the frontal sinuses, computed-tomography (CT) scanning was done to assess for iatrogenic trauma and to determine the degree to which the sinuses were filled with each technique and evaluate the diffusion of the cream into the nasal cavity of each dog...
January 2017: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/28154463/association-of-gingivitis-with-dental-calculus-thickness-or-dental-calculus-coverage-and-subgingival-bacteria-in-feline-leukemia-virus-and-feline-immunodeficiency-virus-negative-cats
#6
Naris Thengchaisri, Jörg M Steiner, Jan S Suchodolski, Panpicha Sattasathuchana
Periodontal disease is the most common oral disease in cats. The objectives of this study were to determine the relationships between gingivitis and dental calculus thickness (DCT), or dental calculus coverage (DCC); determine the association of gingivitis scores and types of oral bacteria; and to evaluate bacterial co-infection in cats with periodontal disease. Twelve cats that were not infected with feline leukemia or feline immunodeficiency viruses were enrolled in the study. Gingivitis, DCT, and DCC were scored and recorded...
January 2017: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/28154462/the-effects-of-oral-administration-of-yunnan-baiyao-on-blood-coagulation-in-beagle-dogs-as-measured-by-kaolin-activated-thromboelastography-and-buccal-mucosal-bleeding-times
#7
Jami Frederick, Søren Boysen, Catherine Wagg, Serge Chalhoub
We examined the effects of oral administration of Yunnan Baiyao (YB) on hemostasis by measuring buccal mucosal bleeding times (BMBTs) and doing citrated kaolin-activated whole-blood thromboelastography (TEG). In a randomized controlled crossover trial 8 beagle dogs were given either placebo or 1000 mg of YB orally every 12 h for 5 consecutive treatments. Blood was drawn 24 h before treatment and 2 and 24 h after the last treatment, and the BMBT was measured in each sample in duplicate. The TEG analysis was done in duplicate 60 ± 5 min after sample collection...
January 2017: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/28154461/evaluation-of-serum-myeloperoxidase-concentration-in-dogs-with-heart-failure-due-to-chronic-mitral-valvular-insufficiency
#8
Jong-In Park, Sang-Il Suh, Changbaig Hyun
Myeloperoxidase (MPO) is a leukocyte-derived enzyme involved in the process of heart failure and is found to have good diagnostic and prognostic values in humans with chronic heart failure. This study evaluated the relationship between serum MPO levels and the severity of heart failure (HF) due to chronic mitral valvular insufficiency (CMVI) in dogs. Eighty-two client-owned dogs consisting of 69 dogs with different stages of HF due to CMVI and 13 age-matched healthy dogs were enrolled in this study. Serum MPO concentrations in the healthy and CMVI groups were determined by enzyme-linked immunosorbent assay (ELISA) using a canine-specific monoclonal anti-MPO antibody...
January 2017: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/28154460/antiviral-activity-of-a-novel-composition-of-peracetic-acid-disinfectant-on-parvoviruses
#9
Fadi Dagher, Jun Jiang, Peter Tijssen, Jean-François Laliberté
Porcine parvoviruses (PPV) are known to be particularly resistant to many disinfectants used to control other non-enveloped viruses. However, effective disinfectants used against PPV are harsh and corrosive to animal health facilities and the environment. We propose a noncorrosive "green" disinfectant that generates peracetic acid in-situ and is capable of inactivating PPV completely at a 1% concentration for a 10-minute contact time.
January 2017: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/28154459/phylogenetic-analysis-of-escherichia-coli-isolated-from-broilers-with-colibacillosis-based-on-gyra-gene-sequences
#10
Hamid Shamsi, Karim Mardani, Abdolghaffar Ownagh
Escherichia coli isolates from chickens with colibacillosis were assigned to phylogenetic groups based on multiplex polymerase chain reaction (PCR) and antibacterial resistance of E. coli belonging to these groups was examined. Furthermore, the gyrA gene of isolates was sequenced and a phylogenetic tree was generated. A total of 84 E. coli isolates were grouped using multiplex PCR of TSPE4.C2, chuA, yjaA, and gadA molecular markers. Four phylogenetic groups were identified with strains divided as follows: 16 in group A (19...
January 2017: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/28154458/analysis-of-efficacy-obtained-with-a-trivalent-inactivated-haemophilus-parasuis-serovars-4-5-and-12-vaccine-and-commercial-vaccines-against-gl%C3%A3-sser-s-disease-in-piglets
#11
Zhanqin Zhao, Huisheng Liu, Yun Xue, Kunpeng Chen, Zhijun Liu, Qiao Xue, Chen Wang
The objective of this study was to assess the efficacy of a trivalent inactivated Haemophilus parasuis serovars 4, 5, and 12 vaccine with polymeric adjuvant gel (GEL) and commercial vaccines against Glässer's disease in piglets. Commercial vaccines containing inactivated H. parasuis serovars 4 and 5 (China), inactivated H. parasuis serovars 1 and 6 (Spain), and inactivated H. parasuis serovar 5 (USA) were also evaluated. Our results demonstrated that the trivalent inactivated H. parasuis serovars 4, 5, and 12 vaccine with GEL adjuvant can provide better protection against the 3 most common pathogenic serovars circulating in China than other commercial vaccines tested...
January 2017: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/28154457/evaluation-of-the-new-commercial-recombinant-chimeric-subunit-vaccine-prrsfree-in-challenge-with-heterologous-types-1-and-2-porcine-reproductive-and-respiratory-syndrome-virus
#12
Jiwoon Jeong, Changhoon Park, Kyuhyung Choi, Chanhee Chae
The objective of this study was to evaluate a new recombinant chimeric vaccine against porcine reproductive and respiratory syndrome virus (PRRSV). The subunit vaccine, PRRSFREE, from Reber Genetics, Taiwan, Republic of China, is based on a plasmid containing a detoxified Pseudomonas exotoxin carrying open reading frame (ORF) 7, 1b, and 5 and 6 chimeric subunits of types 1 and 2 PRRSV. Pigs were injected intramuscularly with 2.0 mL of the vaccine at 21 and 42 d of age, according to the manufacturer's recommendation...
January 2017: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/28154456/induction-of-humoral-immune-response-in-piglets-after-perinatal-or-post-weaning-immunization-against-porcine-circovirus-type-2-or-keyhole-limpet-hemocyanin
#13
Jessica Law, Robert McCorkell, Greg Muench, Katherine Wynne-Edwards, Hermann M Schaetzl, Cristina Solis, Narges Nourozieh, Regula Waeckerlin, Michael Eschbaumer, Shawn Horsman, Markus Czub
The objective of this study was to test the hypothesis that porcine circovirus type-2 (PCV2) vaccination is efficacious when administered in the first week of life. Three groups of pigs were vaccinated with Circumvent either early (at the end of week 1), late (at the end of week 4), or not at all. All 3 groups were later challenged intranasally with PCV2 (at the end of week 5). Two other groups were immunized with keyhole limpet hemocyanin (KLH) as a novel antigen at the end of either week 1 or week 4. Weight, PCV2 genome copy number in serum and saliva, anti-KLH antibody titer, and serum PCV2-neutralizing antibodies were measured weekly...
January 2017: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/28154455/erratum-cjvr-erratum
#14
(no author information available yet)
[This corrects the article on p. 309 in vol. 80, PMID: 27733786.].
January 2017: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/28154454/volume-80-2016
#15
(no author information available yet)
No abstract text is available yet for this article.
January 2017: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/27733789/degree-of-corneal-anesthesia-after-topical-application-of-0-4-oxybuprocaine-ophthalmic-solution-in-normal-equids
#16
RANDOMIZED CONTROLLED TRIAL
Erika Little, Kathy Yvorchuk-St Jean, William Little, Fortune Sithole, Guy St Jean
Oxybuprocaine hydrochloride ophthalmic solution has been widely used off-label in horses and donkeys, despite lack of data demonstrating efficacy and safety in these species. The objective of this study was to assess anesthetic efficacy of 0.4% oxybuprocaine hydrochloride ophthalmic solution in horses (n = 5) and donkeys (n = 24) and compare the effects with 0.5% proparacaine hydrochloride ophthalmic solution. The baseline corneal touch threshold (CTT) was measured with a Cochet-Bonnet esthesiometer. Donkeys (n = 12) and horses (n = 5) in group A received sterile ophthalmic solutions 0...
October 2016: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/27733788/tramadol-does-not-enhance-sedation-induced-by-acepromazine-in-dogs
#17
RANDOMIZED CONTROLLED TRIAL
Eduardo R Monteiro, Renan B Lobo, Juarez S Nunes, Julia P P Rangel, Flavia S Bitti
The sedative effect of acepromazine combined with 2 doses of tramadol [3 and 5 mg/kg body weight (BW)] was compared with the sedative effect of acepromazine alone in dogs and the effects of each sedative protocol on cardiorespiratory variables were examined. This was a prospective, randomized, blinded, crossover study. Each of 6 dogs received 3 treatments at 1-week intervals. During all anesthetic episodes, dogs received 0.05 mg/kg BW acepromazine. Approximately 25 min later, dogs were given physiological saline (control) or tramadol [3 mg/kg BW (TR3) or 5 mg/kg BW (TR5)]...
October 2016: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/27733787/clinical-staging-in-bitches-with-mammary-tumors-influence-of-type-and-histological-grade
#18
Lígia F Gundim, Camila P de Araújo, William T Blanca, Ednaldo C Guimarães, Alessandra A Medeiros
Breast tumors are the most common tumors in dogs and the study of disease prognostic factors is important for establishing the appropriate treatment protocols. The purpose of this study was to clinically stage mammary tumors of bitches and correlate the stages with histological type and grade. The tumors of 63 dogs were clinically staged based on the findings of tumor sizing, lymph node evaluation, and radiographic examination. After surgical excision, the tumors were classified histologically and graded. The relationship between the tumor grade, stage, and histological type was evaluated using a binomial test...
October 2016: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/27733786/identification-and-characterization-of-pig-adipose-derived-progenitor-cells
#19
Shuang Zhang, Chunyu Bai, Dong Zheng, Yuhua Gao, Yanan Fan, Lu Li, Weijun Guan, Yuehui Ma
Adipose-derived stem cells (ADSCs) are multipotent, and can be differentiated into many cell types in vitro. In this study, tissues from pigs were chosen to identify and characterize ADSCs. Primary ADSCs were sub-cultured to passage 28. The surface markers of ADSCs: CD29, CD71, CD73, CD90, and CD166 were detected by reverse-transcription polymerase chain reaction assays and the markers CD29, CD44, CD105, and vimentin were detected by immunofluorescence. Growth curves and the capacity of clone-forming were performed to test the proliferation of ADSCs...
October 2016: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/27733785/evaluation-of-hyaluronic-acid-procollagen-type-iii-n-terminal-peptide-and-tissue-inhibitor-of-matrix-metalloproteinase-1-as-serum-markers-of-canine-hepatic-fibrosis
#20
Jonathan A Lidbury, Aline Rodrigues Hoffmann, Joanna K Fry, Jan S Suchodolski, Jörg M Steiner
The only way to diagnose hepatic fibrosis in dogs is by histological assessment of a liver biopsy specimen. As this technique is invasive and susceptible to sampling variation, serum biomarkers are used to detect hepatic fibrosis in humans. The objective of this study was to assess the utility of hyaluronic acid (HA), procollagen type III N-terminal peptide (PIIINP), and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) as serum markers of canine hepatic fibrosis. Serum samples were collected from 47 dogs with histologically confirmed hepatobiliary disease and 24 healthy dogs in order to measure concentrations of HA, PIIINP, and TIMP-1...
October 2016: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
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