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https://www.readbyqxmd.com/read/28072905/saccharomyces-cerevisiae-shuttle-vectors
#1
REVIEW
Robert Gnügge, Fabian Rudolf
Yeast shuttle vectors are indispensable tools in yeast research. They enable cloning of defined DNA sequences in Escherichia coli and their direct transfer into Saccharomyces cerevisiae cells. There are three types of commonly used yeast shuttle vectors: centromeric plasmids, episomal plasmids and integrating plasmids. In this review, we discuss the different plasmid systems and their characteristic features. We focus on their segregational stability and copy number and indicate how to modify these properties...
January 10, 2017: Yeast
https://www.readbyqxmd.com/read/27943405/n-ice-plasmids-for-generating-n-terminal-3%C3%A2-%C3%A3-%C3%A2-flag-tagged-genes-that-allow-inducible-constitutive-or-endogenous-expression-in-saccharomyces-cerevisiae
#2
Yueping Zhang, Nina D Serratore, Scott D Briggs
PCR-mediated homologous recombination is a powerful approach to introduce epitope tags into the chromosomal loci at the N-terminus or the C-terminus of targeted genes. Although strategies of C-terminal epitope tagging of target genes at their loci are simple and widely used in yeast, C-terminal epitope tagging is not practical for all proteins. For example, a C-terminal tag may affect protein function or a protein may get cleaved or processed resulting in the loss of the epitope tag. Therefore, N-terminal epitope tagging may be necessary to resolve these problems...
December 12, 2016: Yeast
https://www.readbyqxmd.com/read/27930825/modulation-of-meiotic-homologous-recombination-by-dna-helicases
#3
Alexander Lorenz
DNA helicases are ATP-driven motor proteins which translocate along DNA capable of dismantling DNA-DNA interactions and/or removing proteins bound to DNA. These biochemical capabilities make DNA helicases main regulators of crucial DNA metabolic processes, including DNA replication, DNA repair, and genetic recombination. This budding topic will focus on reviewing the function of DNA helicases important for homologous recombination during meiosis, and discuss recent advances in how these modulators of meiotic recombination are themselves regulated...
December 8, 2016: Yeast
https://www.readbyqxmd.com/read/27886402/analysis-of-paracoccidioides-lutzii-mitochondria-a-proteomic-approach
#4
L Casaletti, P S Lima, L N Lima, C L Borges, S N Báo, A M Bailão, C M A Bailão
The genus Paracoccidioides is composed of thermal dimorphic fungi, causative agents of paracoccidioidomycosis, one of the most frequent systemic mycoses in Latin America. Mitochondria have sophisticated machinery for ATP production, which involves metabolic pathways such as citric acid and glyoxylate cycles, electron transport chain and oxidative phosphorylation. In addition, this organelle performs a variety of functions in the cell, working as an exceptional metabolic signaling center that contributes to cellular stress responses, as autophagy and apoptosis in eukaryotic organisms...
November 25, 2016: Yeast
https://www.readbyqxmd.com/read/27862252/specific-populations-of-the-yeast-geotrichum-candidum-revealed-by-molecular-typing
#5
Noémie Jacques, Sandrine Mallet, Fatima Laaghouiti, Colin R Tinsley, Serge Casaregola
Geotrichum candidum is a ubiquitous yeast and an essential component in the production of many soft cheeses. We developed a multilocus sequence typing (MLST) scheme with five retained loci (NUP116, URA1, URA3, SAPT4 and PLB3) which were sufficiently divergent to distinguish 40 sequence types (STs) among the 67 G. candidum strains tested. Phylogenetic analyses defined five main clades; one clade was restricted to environmental isolates, three other clades included distinct environmental isolates and dairy strains, while the fifth clade comprised 34 strains (13 STs), among which all but two were isolated from milk, cheese or the dairy environment...
November 18, 2016: Yeast
https://www.readbyqxmd.com/read/27862271/it-s-not-all-about-us-evolution-and-maintenance-of-cryptococcus-virulence-requires-selection-outside-the-human-host
#6
Aleeza C Gerstein, Kirsten Nielsen
Cryptococcus is predominantly an AIDS-related pathogen that causes significant morbidity and mortality in immunocompromised patients. Research studies have historically focused on understanding how the organism causes human disease through the use of in vivo and in vitro model systems to identify virulence factors. Cryptococcus is not an obligate pathogen, however, as human-human transmission is either absent or rare. Selection in the environment must thus be invoked to shape the evolution of this taxa, and directly influences genotypic and trait diversity...
November 11, 2016: Yeast
https://www.readbyqxmd.com/read/27862269/arp2-3-complex-and-mps3-are-required-for-regulation-of-ribosome-biosynthesis-in-the-secretory-stress-response
#7
Yukari Yabuki, Masako Katayama, Yushi Kodama, Akiko Sakamoto, Ayumi Yatsuhashi, Kouichi Funato, Keiko Mizuta
Secretory defects cause transcriptional repression of ribosome biogenesis in Saccharomyces cerevisiae. However, the molecular mechanism underlying secretory defect-induced transcriptional repression of ribosome biogenesis remains to be fully elucidated. In this study, we demonstrated that the Arp2/3 complex was required for reduction of ribosome protein gene expression in response to defective secretion by addition of tunicamycin. Two cmd1 mutants, cmd1-228 and cmd1-239 that cause mislocalization of calmodulin and defective mitotic spindle formation, respectively, fail to interact with Arc35, a component of the Arp2/3 complex...
November 10, 2016: Yeast
https://www.readbyqxmd.com/read/27862261/cell-surface-hydrophobicity-and-colony-morphology-of-trichosporon-asahii-clinical-isolates
#8
Tomoe Ichikawa, Chihiro Hirata, Mizuki Takei, Naoyuki Tagami, Hiromi Murasawa, Reiko Ikeda
Trichosporon asahii is a pathogenic basidiomycetous yeast. Individual strains of T. asahii have different colony morphologies. However, it is not clear whether cell surface phenotypes differ among the colony morphologies. Here we characterized the cell surface hydrophobicity and analysed the carbohydrate contents of the cell surface polysaccharides in T. asahii clinical isolates with various colony morphologies. Among the three distinctive colony morphologies obtained from one clinical isolate, the white-type morphology exhibited higher hydrophobicity...
November 8, 2016: Yeast
https://www.readbyqxmd.com/read/27813152/yeasts-found-in-vineyards-and-wineries
#9
Cristian Varela, Anthony R Borneman
Wine is a complex beverage, comprising thousands of metabolites that are produced through the action of a plethora of yeasts and bacteria during fermentation of grape must. These microbial communities originate in the vineyard and the winery and reflect the influence of several factors including grape variety, geographical location, climate, vineyard spraying, technological practices, processing stage and season (pre-harvest, harvest, post-harvest). Vineyard and winery microbial communities have the potential to participate during fermentation and influence wine flavour and aroma...
November 3, 2016: Yeast
https://www.readbyqxmd.com/read/27794167/naumovozyma-castellii-an-alternative-model-for-budding-yeast-molecular-biology
#10
Ahu Karademir Andersson, Marita Cohn
Naumovozyma castellii (Saccharomyces castellii) is a member of the budding yeast family Saccharomycetaceae. It has been extensively used as a model organism for telomere biology research and has gained increasing interest as a budding yeast model for functional analyses owing to its amenability to genetic modifications. Owing to the suitable phylogenetic distance to S. cerevisiae, the whole genome sequence of N. castellii has provided unique data for comparative genomic studies, and it played a key role in the establishment of the timing of the whole genome duplication and the evolutionary events that took place in the subsequent genomic evolution of the Saccharomyces lineage...
October 28, 2016: Yeast
https://www.readbyqxmd.com/read/28084030/use-of-a-fluoride-channel-as-a-new-selection-marker-for-fission-yeast-plasmids-and-application-to-fast-genome-editing-with-crispr-cas9
#11
(no author information available yet)
No abstract text is available yet for this article.
January 2017: Yeast
https://www.readbyqxmd.com/read/27669110/development-of-stable-haploid-strains-and-molecular-genetic-tools-for-naumovozyma-castellii-saccharomyces-castellii
#12
Ahu Karademir Andersson, Stina Oredsson, Marita Cohn
The budding yeast Naumovozyma castellii (syn. Saccharomyces castellii) has been included in comparative genomics studies and functional analyses of centromere DNA elements, and has been shown to possess beneficial traits for telomere biology research. To provide useful tools for molecular genetic approaches, we produced stable haploid heterothallic strains from an early ancestral strain derived from the N. castellii collection strain CBS 4310. To this end, we deleted the gene encoding the Ho endonuclease, which is essential for the mating type switching...
December 2016: Yeast
https://www.readbyqxmd.com/read/27668979/erg6-gene-deletion-modifies-kluyveromyces-lactis-susceptibility-to-various-growth-inhibitors
#13
Alexandra Konecna, Nora Toth Hervay, Martin Valachovic, Yvetta Gbelska
The ERG6 gene encodes an S-adenosylmethionine dependent sterol C-24 methyltransferase in the ergosterol biosynthetic pathway. In this work we report the results of functional analysis of the Kluyveromyces lactis ERG6 gene. We cloned the KlERG6 gene, which was able to complement the erg6Δ mutation in both K. lactis and Saccharomyces cerevisiae. The lack of ergosterol in the Klerg6 deletion mutant was accompanied by increased expression of genes encoding the last steps of the ergosterol biosynthesis pathway as well as the KlPDR5 gene encoding an ABC transporter...
December 2016: Yeast
https://www.readbyqxmd.com/read/27654890/use-of-ade1-and-ade2-mutations-for-development-of-a-versatile-red-white-colour-assay-of-amyloid-induced-oxidative-stress-in-saccharomyces-cerevisiae
#14
Vidhya Bharathi, Amandeep Girdhar, Archana Prasad, Meenkshi Verma, Vibha Taneja, Basant K Patel
Mutations in adenine biosynthesis pathway genes ADE1 and ADE2 have been conventionally used to score for prion [PSI(+) ] in yeast. If ade1-14 mutant allele is present, which contains a premature stop codon, [psi(-) ] yeast appear red on YPD medium owing to accumulation of a red intermediate compound in vacuoles. In [PSI(+) ] yeast, partial inactivation of the translation termination factor, Sup35 protein, owing to its amyloid aggregation allows for read-through of the ade1-14 stop codon and the yeast appears white as the red intermediate pigment is not accumulated...
December 2016: Yeast
https://www.readbyqxmd.com/read/27766670/spatial-organization-of-the-schizosaccharomyces-pombe-genome-within-the-nucleus
#15
Atsushi Matsuda, Haruhiko Asakawa, Tokuko Haraguchi, Yasushi Hiraoka
The fission yeast Schizosaccharomyces pombe is a useful experimental system for studying the organization of chromosomes within the cell nucleus. S. pombe has a small genome that is organized into three chromosomes. The small size of the genome and the small number of chromosomes are advantageous for cytological and genome-wide studies of chromosomes; however, the small size of the nucleus impedes microscopic observations owing to limits in spatial resolution during imaging. Recent advances in microscopy, such as super-resolution microscopy, have greatly expanded the use of S...
October 21, 2016: Yeast
https://www.readbyqxmd.com/read/27743491/characterization-of-phosphatidic-acid-phosphatase-activity-in-the-oleaginous-yeast-yarrowia-lipolytica-and-its-role-in-lipid-biosynthesis
#16
Derell Hardman, Daniel McFalls, Stylianos Fakas
Phosphatidic acid phosphatase (PAP) catalyses the committed step of triacylglycerol (TAG) biosynthesis and thus regulates the amounts of TAG produced by the cell. TAG is the target of biotechnological processes developed for the production of food lipids or biofuels. These processes are using oleaginous microorganisms like the yeast Yarrowia lipolytica as the TAG producers. Thus manipulating key enzymatic activities like PAP in Y. lipolytica could drive lipid biosynthesis towards TAG production and increase TAG yields...
October 15, 2016: Yeast
https://www.readbyqxmd.com/read/27743455/opi1-mediates-repression-of-phospholipid-biosynthesis-by-phosphate-limitation-in-the-yeast-saccharomyces-cerevisiae
#17
Felix Kliewe, Jacqueline Kumme, Mathias Grigat, Stefan Hintze, Hans-Joachim Schüller
Structural genes of phospholipid biosynthesis in the yeast S. cerevisiae are transcribed when precursor molecules inositol and choline (IC) are limiting. Gene expression is stimulated by the heterodimeric activator Ino2/Ino4 which binds to ICRE (inositol/choline-responsive element) promoter sequences. Activation is prevented by repressor Opi1, counteracting Ino2 when high concentrations of IC are available. Here we show that ICRE-dependent gene activation is not only repressed by an excess of IC but also under conditions of phosphate starvation...
October 15, 2016: Yeast
https://www.readbyqxmd.com/read/27714848/the-regulatable-mal32-promoter-in-saccharomyces-cerevisiae-characteristics-and-tools-to-facilitate-its-use
#18
Matthias Meurer, Veronika Chevyreva, Bram Cerulus, Michael Knop
Here we describe a set of tools to facilitate the use of maltose and the MAL32 promoter for regulated gene expression in yeast, alone or in combination with the GAL1 promoter. Using fluorescent protein reporters we find that under non-inducing conditions the MAL32 promoter exhibits a low basal level of expression, similar to the GAL1 promoter, and that both promoters can be induced independently of each other using the respective sugars, maltose and galactose. While their repression upon glucose addition is immediate and complete, we found that the MAL32 and GAL1 promoters each exhibit distinct induction kinetics...
October 6, 2016: Yeast
https://www.readbyqxmd.com/read/27327046/use-of-a-fluoride-channel-as-a-new-selection-marker-for-fission-yeast-plasmids-and-application-to-fast-genome-editing-with-crispr-cas9
#19
Ronan Fernandez, Julien Berro
Fission yeast is a powerful model organism that has provided insights into important cellular processes thanks to the ease of its genome editing by homologous recombination. However, creation of strains with a large number of targeted mutations or containing plasmids has been challenging because only a very small number of selection markers is available in Schizosaccharomyces pombe. In this paper, we identify two fission yeast fluoride exporter channels (Fex1p and Fex2p) and describe the development of a new strategy using Fex1p as a selection marker for transformants in rich media supplemented with fluoride...
October 2016: Yeast
https://www.readbyqxmd.com/read/27668839/proteomic-and-genomic-characterization-of-a-yeast-model-for-ogden-syndrome
#20
Max J Dörfel, Han Fang, Jonathan Crain, Michael Klingener, Jake Weiser, Gholson J Lyon
Naa10 is an N(α) -terminal acetyltransferase that, in a complex with its auxiliary subunit Naa15, co-translationally acetylates the α-amino group of newly synthetized proteins as they emerge from the ribosome. Roughly 40-50% of the human proteome is acetylated by Naa10, rendering this an enzyme one of the most broad substrate ranges known. Recently, we reported an X-linked disorder of infancy, Ogden syndrome, in two families harbouring a c.109 T > C (p.Ser37Pro) variant in NAA10. In the present study we performed in-depth characterization of a yeast model of Ogden syndrome...
September 26, 2016: Yeast
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