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https://www.readbyqxmd.com/read/28732129/%C3%A3-1-6-glucan-synthesis-associated-genes-are-required-for-proper-spore-wall-formation-in-saccharomyces-cerevisiae
#1
Hua-Ping Pan, Ning Wang, Hiroyuki Tachikawa, Hideki Nakanishi, Xiao-Dong Gao
The yeast spore wall is an excellent model to study the assembly of an extracellular macromolecule structure. In the present study, mutants defective in ß-1,6-glucan synthesis, including kre1∆, kre6∆, kre9∆, and big1∆, were sporulated to analyze the effect of ß-1,6-glucan defects on the spore wall. Except for kre6∆, these mutant spores were sensitive to treatment with ether, suggesting that the mutations perturb the integrity of the spore wall. Morphologically, the mutant spores were indistinguishable from wild-type spores...
July 21, 2017: Yeast
https://www.readbyqxmd.com/read/28732117/identification-by-phenotypic-and-genetic-approaches-of-an-indigenous-saccharomyces-cerevisiae-wine-strain-with-high-desiccation-tolerance
#2
Marianna Zambuto, Rossana Romaniello, Nicoletta Guaragnella, Patrizia Romano, Sonia Votta, Angela Capece
During active dry yeast (ADY) production process, cells are exposed to multiple stresses, such as thermal, oxidative and hyperosmotic shock. Previously, by analyzing cells in exponential growth phase, we selected an indigenous Saccharomyces cerevisiae wine strain, namely CD-6Sc, for its higher tolerance to desiccation and higher expression of specific desiccation stress-related genes in comparison to other yeast strains. In this study, we performed a desiccation treatment on stationary phase cells by comparing the efficacy of two different methods: a "laboratory dry test" in a small-scale (mild stress) and a treatment by spray-drying (severe stress), one of the most appropriate preservation method for yeasts and other micro-organisms...
July 21, 2017: Yeast
https://www.readbyqxmd.com/read/28681487/division-of-labour-in-the-yeast-saccharomyces-cerevisiae
#3
Dominika M Wloch-Salamon, Roberta M Fisher, Birgitte Regenberg
Division of labour between different specialized cell types is a central part of how we describe complexity in multicellular organisms. However, it is increasingly being recognised that division of labour also plays an important role in the lives of predominantly unicellular organisms. Saccharomyces cerevisiae displays several phenotypes that could be considered a division of labour, including quiescence, apoptosis and biofilm formation, but they have not been explicitly treated as such. We discuss each of these examples, using a definition of division of labour that involves phenotypic variation between cells within a population, cooperation between cells performing different tasks, and maximisation of the inclusive fitness of all cells involved...
July 5, 2017: Yeast
https://www.readbyqxmd.com/read/28681409/hybridization-and-emergence-of-virulence-in-opportunistic-human-yeast-pathogens
#4
Verónica Mixão, Toni Gabaldón
Hybridization between different species can result in the emergence of new lineages and adaptive phenotypes. Occasionally, hybridization in fungal organisms can drive the appearance of opportunistic lifestyles or shifts to new hosts, resulting in the emergence of novel pathogens. In recent years, an increasing number of studies have documented the existence of hybrids in diverse yeast clades, including some comprising human pathogens. Comparative and population genomics studies performed on these clades are enabling us to understand what roles hybridization may play in the evolution and emergence of a virulence potential towards humans...
July 5, 2017: Yeast
https://www.readbyqxmd.com/read/28581036/opi1p-translocation-to-the-nucleus-is-regulated-by-hydrogen-peroxide-in-saccharomyces-cerevisiae
#5
Carolina Camelo, Filipe Vilas-Boas, Andreia Pereira Cepeda, Carla Real, Joana Barros-Martins, Francisco Pinto, Helena Soares, H Susana Marinho, Luisa Cyrne
During exposure of yeast cells to low levels of hydrogen peroxide (H2 O2 ), the expression of several genes is regulated for cells to adapt to the surrounding oxidative environment. Such adaptation involves modification of plasma membrane lipid composition, reorganization of ergosterol-rich microdomains and altered gene expression of proteins involved in lipid and vesicle traffic, to decrease permeability to exogenous H2 O2 . Opi1p is a transcriptional repressor that is inactive when present at the nuclear membrane/endoplasmic reticulum, but represseses transcription of inositol upstream activating sequence (UASINO )-containing genes, many of which are involved in the synthesis of phospholipids and fatty acids, when it is translocated to the nucleus...
June 5, 2017: Yeast
https://www.readbyqxmd.com/read/28568773/cost-effective-and-rapid-lysis-of-saccharomyces-cerevisiae-cells-for-quantitative-western-blot-analysis-of-proteins-including-phosphorylated-eif2%C3%AE
#6
Su Jung Lee, Rashmi Ramesh, Valerie de Boor, Jan M Gebler, Richard C Silva, Evelyn Sattlegger
The common method for liberating proteins from Saccharomyces cerevisiae cells involves mechanical cell disruption using glass beads and buffer containing inhibitors (protease, phosphatase, and/or kinase inhibitors), followed by centrifugation to remove cell debris. This procedure requires the use of costly inhibitors, and is laborious, in particular when many samples need to be processed. Also, enzymatic reactions can still occur during harvesting and cell breakage. As a result low abundant and labile proteins may be degraded, and enzymes such as kinases and phosphatases may still modify proteins during and after cell lysis...
June 1, 2017: Yeast
https://www.readbyqxmd.com/read/28556381/the-spoilage-yeast-zygosaccharomyces-bailii-foe-or-friend
#7
Nurzhan Kuanyshev, Giusy M Adamo, Danilo Porro, Paola Branduardi
Zygosaccharomyces bailii is a non-Saccharomyces budding yeast known as one of the most aggressive food spoilage microorganism, often isolated as contaminant during wine fermentation, as well as from many acidic, high sugar and canned foods. The spoilage ability relies on the yeast's unique feature to tolerate the most common preservatives such as sulfite, dimethyl dicarbonate, acetic acid, and sorbic acid. Therefore, many studies focused on the description of this peculiar tolerance with the aim of developing preventative measures against Z...
May 27, 2017: Yeast
https://www.readbyqxmd.com/read/28207166/a-set-of-isomeric-episomal-plasmids-for-systematic-examination-of-mitotic-stability-in-saccharomyces-cerevisiae
#8
Ruben Hohnholz, Kim Julia Pohlmann, Tilman Achstetter
Yeast episomal shuttle vectors (YEp type) are commonly used in fundamental research and biotechnology whenever elevated product levels are desired. Their instability, however, poses an impediment not only in industrial scale fermentation. In order to analyse instability which might be linked to plasmid structure, a series of YEp type plasmids that are identical in size has been assembled, differing only in the overall arrangement of the fragments used. The performance of the eight plasmid isoforms was studied with respect to mitotic stability...
June 2017: Yeast
https://www.readbyqxmd.com/read/28160314/saccharomyces-cerevisiae-atf1p-is-an-alcohol-acetyltransferase-and-a-thioesterase-in-vitro
#9
Bethany Nancolas, Ian D Bull, Richard Stenner, Virginie Dufour, Paul Curnow
The alcohol-O-acyltransferases are bisubstrate enzymes that catalyse the transfer of acyl chains from an acyl-coenzyme A (CoA) donor to an acceptor alcohol. In the industrial yeast Saccharomyces cerevisiae this reaction produces acyl esters that are an important influence on the flavour of fermented beverages and foods. There is also a growing interest in using acyltransferases to produce bulk quantities of acyl esters in engineered microbial cell factories. However, the structure and function of the alcohol-O-acyltransferases remain only partly understood...
June 2017: Yeast
https://www.readbyqxmd.com/read/28072905/saccharomyces-cerevisiae-shuttle-vectors
#10
REVIEW
Robert Gnügge, Fabian Rudolf
Yeast shuttle vectors are indispensable tools in yeast research. They enable cloning of defined DNA sequences in Escherichia coli and their direct transfer into Saccharomyces cerevisiae cells. There are three types of commonly used yeast shuttle vectors: centromeric plasmids, episomal plasmids and integrating plasmids. In this review, we discuss the different plasmid systems and their characteristic features. We focus on their segregational stability and copy number and indicate how to modify these properties...
May 2017: Yeast
https://www.readbyqxmd.com/read/27943405/n-ice-plasmids-for-generating-n-terminal-3%C3%A2-%C3%A3-%C3%A2-flag-tagged-genes-that-allow-inducible-constitutive-or-endogenous-expression-in-saccharomyces-cerevisiae
#11
Yueping Zhang, Nina D Serratore, Scott D Briggs
PCR-mediated homologous recombination is a powerful approach to introduce epitope tags into the chromosomal loci at the N-terminus or the C-terminus of targeted genes. Although strategies of C-terminal epitope tagging of target genes at their loci are simple and widely used in yeast, C-terminal epitope tagging is not practical for all proteins. For example, a C-terminal tag may affect protein function or a protein may get cleaved or processed, resulting in the loss of the epitope tag. Therefore, N-terminal epitope tagging may be necessary to resolve these problems...
May 2017: Yeast
https://www.readbyqxmd.com/read/27930825/modulation-of-meiotic-homologous-recombination-by-dna-helicases
#12
Alexander Lorenz
DNA helicases are ATP-driven motor proteins which translocate along DNA capable of dismantling DNA-DNA interactions and/or removing proteins bound to DNA. These biochemical capabilities make DNA helicases main regulators of crucial DNA metabolic processes, including DNA replication, DNA repair, and genetic recombination. This budding topic will focus on reviewing the function of DNA helicases important for homologous recombination during meiosis, and discuss recent advances in how these modulators of meiotic recombination are themselves regulated...
May 2017: Yeast
https://www.readbyqxmd.com/read/28426167/characterization-the-carotenoid-productions-and-profiles-of-three-rhodosporidium-toruloides-mutants-from-agrobacterium-tumefaciens-mediated-transformation
#13
Xinping Lin, Ning Gao, Sasa Liu, Sufang Zhang, Shuang Song, Chaofan Ji, Xiuping Dong, Yichen Su, Zongbao Kent Zhao, Beiwei Zhu
The red yeast Rhodosporidium toruloides is a known lipid producer capable of accumulating large amounts of triacylglycerols and carotenoids. However, it remains challenging to study its carotenoid production profiles due to limited biochemical information and inefficient genetic tools. Here we used an Agrobacterium tumefaciens-mediated transformation (ATMT) to change its carotenoid production and profiles. We constructed R. toruloides NP11 mutant libraries with ATMT, selected 3 mutants with different colors, characterized their carotenoid products by high pressure liquid chromatography-mass spectrometry (HPLC-MS) analysis, and assured differences among those strains in terms of carotenoid production and its composition profiles...
April 20, 2017: Yeast
https://www.readbyqxmd.com/read/28426144/a-multiplex-culture-system-for-the-long-term-growth-of-fission-yeast-cells
#14
Céline Callens, Nelson C Coelho, Aaron W Miller, Maria Rosa Domingo Sananes, Maitreya J Dunham, Matthieu Denoual, Damien Coudreuse
Maintenance of long-term cultures of yeast cells is central to a broad range of investigations, from metabolic studies to laboratory evolution assays. However, repeated dilutions of batch cultures lead to variations in medium composition, with implications for cell physiology. In Saccharomyces cerevisiae, powerful miniaturized chemostat setups, or ministat arrays, have been shown to allow for constant dilution of multiple independent cultures. Here we set out to adapt these arrays for continuous culture of a morphologically and physiologically distinct yeast, the fission yeast Schizosaccharomyces pombe, with the goal of maintaining constant population density over time...
April 20, 2017: Yeast
https://www.readbyqxmd.com/read/28423198/an-estradiol-inducible-promoter-enables-fast-graduated-control-of-gene-expression-in-fission-yeast
#15
Makoto J Ohira, David G Hendrickson, R Scott McIsaac, Nicholas Rhind
The fission yeast Schizosaccharomyces pombe lacks a diverse toolkit of inducible promoters for experimental manipulation. Available inducible promoters suffer from slow induction kinetics, limited control of expression levels and/or a requirement for defined growth medium. In particular, no S. pombe inducible promoter systems exhibit a linear dose-response, which would allow expression to be tuned to specific levels. We have adapted a fast, orthogonal promoter system with a large dynamic range and a linear dose response, based on β-estradiol-regulated function of the human oestrogen receptor, for use in S...
April 19, 2017: Yeast
https://www.readbyqxmd.com/read/28387035/the-frenemies-within-viruses-retrotransposons-and-plasmids-that-naturally-infect-saccharomyces-yeasts
#16
Paul A Rowley
Viruses are a major focus of current research efforts because of their detrimental impact on humanity and their ubiquity within the environment. Bacteriophages have long been used to study host-virus interactions within microbes, but it is often forgotten that the single-celled eukaryote Saccharomyces cerevisiae and related species are infected with double-stranded RNA viruses, single-stranded RNA viruses, LTR-retrotransposons and double-stranded DNA plasmids. These intracellular nucleic acid elements have some similarities to higher eukaryotic viruses, i...
April 7, 2017: Yeast
https://www.readbyqxmd.com/read/28384382/the-neutral-n-linked-glycans-of-the-ustilaginomycete-yeast-sympodiomycopsis-paphiopedili
#17
Ronilo Jose D Flores, Takao Ohashi, Hiroko Kawasaki, Kazuhito Fujiyama
Sympodiomycopsis paphiopedili is a basidiomycetous yeast under the subphylum Ustilaginomycotina and is a commensal organism originally isolated from the nectar of a plant species in Japan. In this study, the neutral N-linked glycans of S. paphiopedili were prepared and structurally analysed using high-performance liquid chromatography (HPLC) and mass spectrometry (MS). Glycosidase digestion analyses were also performed to verify certain glycan linkages. HPLC and MS analyses revealed the presence of neutral N-linked glycans ranging from Man3 GlcNAc2 -PA to Man9 GlcNAc2 -PA in length...
April 6, 2017: Yeast
https://www.readbyqxmd.com/read/28303649/transforming-sugars-into-fat-lipid-biosynthesis-using-different-sugars-in-yarrowia-lipolytica
#18
Piotr Hapeta, Magdalena Rakicka, Remi Dulermo, Heber Gamboa-Meléndez, Anne-Marie Cruz-Le Coq, Jean-Marc Nicaud, Zbigniew Lazar
In an era of ever-increasing energy demands, a promising technology is being developed: the use of oleaginous microorganisms such as Yarrowia lipolytica to convert waste materials into biofuels. Here, we constructed two Y. lipolytica strains that displayed both increased lipid accumulation and more efficient use of biomass-derived sugars, including glucose, fructose, galactose and inulin. The first strain, Y. lipolytica YLZ150, was derived from the French wild-type strain W29. It had inhibited triacylglycerol mobilization (∆tgl4) and β-oxidation (∆pox1-6), and it overexpressed GPD1, DGA2, HXK1, the native Leloir pathway, SUC2 from Saccharomyces cerevisiae and INU1 from Kluyveromyces marxianus...
March 17, 2017: Yeast
https://www.readbyqxmd.com/read/28181315/emodin-a-natural-inhibitor-of-protein-kinase-ck2-suppresses-growth-hyphal-development-and-biofilm-formation-of-candida-albicans
#19
Monika Janeczko, Maciej Masłyk, Konrad Kubiński, Hieronim Golczyk
Emodin (1,3,8-trihydroxy-6-methyl-anthraquinone) is a natural secondary plant product, originally isolated from the rhizomes of Rheum palmatum. Many reports show its diuretic, vasorelaxant, antibacterial, antiviral, anti-ulcerogenic, immunosuppressive, hepatoprotective, anti-inflammatory and anticancer potential. Emodin is a pleiotropic molecule capable of interacting with several major molecular targets, e.g. NF-κB, AKT/mTOR and STAT3. The compound can also act as an inhibitor of some protein kinases, with special affinity to protein kinase CK2...
February 8, 2017: Yeast
https://www.readbyqxmd.com/read/28084030/use-of-a-fluoride-channel-as-a-new-selection-marker-for-fission-yeast-plasmids-and-application-to-fast-genome-editing-with-crispr-cas9
#20
(no author information available yet)
No abstract text is available yet for this article.
January 2017: Yeast
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