journal
MENU ▼
Read by QxMD icon Read
search

Yeast

journal
https://www.readbyqxmd.com/read/27886402/analysis-of-paracoccidioides-lutzii-mitochondria-a-proteomic-approach
#1
L Casaletti, P S Lima, L N Lima, C L Borges, S N Báo, A M Bailão, C M A Bailão
The genus Paracoccidioides is composed of thermal dimorphic fungi, causative agents of paracoccidioidomycosis, one of the most frequent systemic mycoses in Latin America. Mitochondria have sophisticated machinery for ATP production, which involves metabolic pathways such as citric acid and glyoxylate cycles, electron transport chain and oxidative phosphorylation. In addition, this organelle performs a variety of functions in the cell, working as an exceptional metabolic signaling center that contributes to cellular stress responses, as autophagy and apoptosis in eukaryotic organisms...
November 25, 2016: Yeast
https://www.readbyqxmd.com/read/27862252/specific-populations-of-the-yeast-geotrichum-candidum-revealed-by-molecular-typing
#2
Noémie Jacques, Sandrine Mallet, Fatima Laaghouiti, Colin R Tinsley, Serge Casaregola
Geotrichum candidum is an ubiquitous yeast, and an essential component in the production of many soft cheeses. We developed a Multi-Locus Sequence Typing (MLST) scheme with five retained loci (NUP116, URA1, URA3, SAPT4, PLB3) which were sufficiently divergent to distinguish 40 Sequence Types (STs) among the 67 G. candidum strains tested. Phylogenetic analyses defined five main clades; one clade was restricted to environmental isolates, three other clades included distinct environmental isolates and dairy strains, while the fifth clade comprised 34 strains (13 STs), among which all but two were isolated from milk, cheese or dairy environment...
November 18, 2016: Yeast
https://www.readbyqxmd.com/read/27862271/it-s-not-all-about-us-evolution-and-maintenance-of-cryptococcus-virulence-requires-selection-outside-the-human-host
#3
Aleeza C Gerstein, Kirsten Nielsen
Cryptococcus is predominantly an AIDS-related pathogen that causes significant morbidity and mortality in immunocompromised patients. Research studies have historically focused on understanding how the organism causes human disease through the use of in vivo and in vitro model systems to identify virulence factors. Cryptococcus is not an obligate pathogen, however, as human-human transmission is either absent or rare. Selection in the environment must thus be invoked to shape the evolution of this taxa, and directly influences genotypic and trait diversity...
November 11, 2016: Yeast
https://www.readbyqxmd.com/read/27862269/arp2-3-complex-and-mps3-are-required-for-regulation-of-ribosome-biosynthesis-in-the-secretory-stress-response
#4
Yukari Yabuki, Masako Katayama, Yushi Kodama, Akiko Sakamoto, Ayumi Yatsuhashi, Kouichi Funato, Keiko Mizuta
Secretory defects cause transcriptional repression of ribosome biogenesis in Saccharomyces cerevisiae. However, the molecular mechanism underlying secretory defect-induced transcriptional repression of ribosome biogenesis remains to be fully elucidated. In this study, we demonstrated that the Arp2/3 complex was required for reduction of ribosome protein gene expression in response to defective secretion by addition of tunicamycin. Two cmd1 mutants, cmd1-228 and cmd1-239 that cause mislocalization of calmodulin and defective mitotic spindle formation, respectively, fail to interact with Arc35, a component of the Arp2/3 complex...
November 10, 2016: Yeast
https://www.readbyqxmd.com/read/27862261/cell-surface-hydrophobicity-and-colony-morphology-of-trichosporon-asahii-clinical-isolates
#5
Tomoe Ichikawa, Chihiro Hirata, Mizuki Takei, Naoyuki Tagami, Hiromi Murasawa, Reiko Ikeda
Trichosporon asahii is a pathogenic basidiomycetous yeast. Individual strains of T. asahii have different colony morphologies. However, it is not clear whether cell surface phenotypes differ among the colony morphologies. Here we characterized the cell surface hydrophobicity and analysed the carbohydrate contents of the cell surface polysaccharides in T. asahii clinical isolates with various colony morphologies. Among the three distinctive colony morphologies obtained from one clinical isolate, the white-type morphology exhibited higher hydrophobicity...
November 8, 2016: Yeast
https://www.readbyqxmd.com/read/27813152/yeasts-found-in-vineyards-and-wineries
#6
Cristian Varela, Anthony R Borneman
Wine is a complex beverage, comprising thousands of metabolites that are produced through the action of a plethora of yeasts and bacteria during fermentation of grape must. These microbial communities originate in the vineyard and the winery and reflect the influence of several factors including grape variety, geographical location, climate, vineyard spraying, technological practices, processing stage and season (pre-harvest, harvest, post-harvest). Vineyard and winery microbial communities have the potential to participate during fermentation and influence wine flavour and aroma...
November 3, 2016: Yeast
https://www.readbyqxmd.com/read/27794167/naumovozyma-castellii-an-alternative-model-for-budding-yeast-molecular-biology
#7
Ahu Karademir Andersson, Marita Cohn
Naumovozyma castellii (Saccharomyces castellii) is a member of the budding yeast family Saccharomycetaceae. It has been extensively used as a model organism for telomere biology research and has gained increasing interest as a budding yeast model for functional analyses owing to its amenability to genetic modifications. Owing to the suitable phylogenetic distance to S. cerevisiae, the whole genome sequence of N. castellii has provided unique data for comparative genomic studies, and it played a key role in the establishment of the timing of the whole genome duplication and the evolutionary events that took place in the subsequent genomic evolution of the Saccharomyces lineage...
October 28, 2016: Yeast
https://www.readbyqxmd.com/read/27766670/spatial-organization-of-the-schizosaccharomyces-pombe-genome-within-the-nucleus
#8
Atsushi Matsuda, Haruhiko Asakawa, Tokuko Haraguchi, Yasushi Hiraoka
The fission yeast Schizosaccharomyces pombe is a useful experimental system for studying the organization of chromosomes within the cell nucleus. S. pombe has a small genome that is organized into three chromosomes. The small size of the genome and the small number of chromosomes are advantageous for cytological and genome-wide studies of chromosomes; however, the small size of the nucleus impedes microscopic observations owing to limits in spatial resolution during imaging. Recent advances in microscopy, such as super-resolution microscopy, have greatly expanded the use of S...
October 21, 2016: Yeast
https://www.readbyqxmd.com/read/27743491/characterization-of-phosphatidic-acid-phosphatase-activity-in-the-oleaginous-yeast-yarrowia-lipolytica-and-its-role-in-lipid-biosynthesis
#9
Derell Hardman, Daniel McFalls, Stylianos Fakas
Phosphatidic acid phosphatase (PAP) catalyzes the committed step of triacylglycerol (TAG) biosynthesis and thus regulates the amounts of TAG produced by the cell. TAG is the target of biotechnological processes developed for the production of food lipids or biofuels. These processes are using oleaginous microorganisms like the yeast Yarrowia lipolytica as the TAG producers. Thus manipulating key enzymatic activities like PAP in Y. lipolytica could drive lipid biosynthesis towards TAG production and increase TAG yields...
October 15, 2016: Yeast
https://www.readbyqxmd.com/read/27743455/opi1-mediates-repression-of-phospholipid-biosynthesis-by-phosphate-limitation-in-the-yeast-saccharomyces-cerevisiae
#10
Felix Kliewe, Jacqueline Kumme, Mathias Grigat, Stefan Hintze, Hans-Joachim Schüller
Structural genes of phospholipid biosynthesis in the yeast S. cerevisiae are transcribed when precursor molecules inositol and choline (IC) are limiting. Gene expression is stimulated by the heterodimeric activator Ino2/Ino4 which binds to ICRE (inositol/choline-responsive element) promoter sequences. Activation is prevented by repressor Opi1, counteracting Ino2 when high concentrations of IC are available. Here we show that ICRE-dependent gene activation is not only repressed by an excess of IC but also under conditions of phosphate starvation...
October 15, 2016: Yeast
https://www.readbyqxmd.com/read/27714848/the-regulatable-mal32-promoter-in-s-cerevisiae-characteristics-and-tools-to-facilitate-its-use
#11
Matthias Meurer, Veronika Chevyreva, Bram Cerulus, Michael Knop
Here we describe a set of tools to facilitate the use of maltose and the MAL32 promoter for regulated gene expression in yeast, alone or in combination with the GAL1 promoter. Using fluorescent protein reporters we find that under non-inducing conditions the MAL32 promoter exhibits a low basal level of expression, similar to the GAL1 promoter, and that both promoters can be induced independently of each other using the respective sugars, maltose and galactose. While their repression upon glucose addition is immediate and complete, we found that the MAL32 and GAL1 promoter each exhibit distinct induction kinetics...
October 6, 2016: Yeast
https://www.readbyqxmd.com/read/27669110/development-of-stable-haploid-strains-and-molecular-genetic-tools-for-naumovozyma-castellii-saccharomyces-castellii
#12
Ahu Karademir Andersson, Stina Oredsson, Marita Cohn
The budding yeast Naumovozyma castellii (syn. Saccharomyces castellii) has been included in comparative genomics studies, functional analyses of centromere DNA elements, and was shown to possess beneficial traits for telomere biology research. To provide useful tools for molecular genetic approaches, we produced stable haploid heterothallic strains from an early ancestral strain derived from the N. castellii collection strain CBS 4310. To this end, we deleted the gene encoding the Ho endonuclease, which is essential for the mating type switching...
September 26, 2016: Yeast
https://www.readbyqxmd.com/read/27668979/erg6-gene-deletion-modifies-kluyveromyces-lactis-susceptibility-to-various-growth-inhibitors
#13
Alexandra Konecna, Nora Toth Hervay, Martin Valachovic, Yvetta Gbelska
The ERG6 gene encodes an S-adenosylmethionine dependent sterol C-24 methyltransferase in the ergosterol biosynthetic pathway. In this work we report the results of functional analysis of the Kluyveromyces lactis ERG6 gene. We cloned the KlERG6 gene, which was able to complement the erg6Δ mutation in both K. lactis and Saccharomyces cerevisiae. The lack of ergosterol in the Klerg6 deletion mutant was accompanied by increased expression of genes encoding the last steps of the ergosterol biosynthesis pathway as well as the KlPDR5 gene encoding an ABC transporter...
September 26, 2016: Yeast
https://www.readbyqxmd.com/read/27668839/proteomic-and-genomic-characterization-of-a-yeast-model-for-ogden-syndrome
#14
Max J Dörfel, Han Fang, Jonathan Crain, Michael Klingener, Jake Weiser, Gholson J Lyon
Naa10 is a N(α) -terminal acetyltransferase that, in a complex with its auxiliary subunit Naa15, co-translationally acetylates the α-amino group of newly synthetized proteins as they emerge from the ribosome. Roughly 40-50% of the human proteome is acetylated by Naa10, rendering this an enzyme with one of the most broad substrate ranges known. Recently, we reported an X-linked disorder of infancy, Ogden syndrome, in two families harboring a c.109 T > C (p.Ser37Pro) variant in NAA10. In the present study we performed in-depth characterization of a yeast model of Ogden syndrome...
September 26, 2016: Yeast
https://www.readbyqxmd.com/read/27668700/natural-gene-expression-variation-studies-in-yeast
#15
Dawn A Thompson, Francisco A Cubillos
The rise of sequence information across different yeast species and strains is driving an increasing number of studies in the emerging field of genomics to associate polymorphic variants - mRNA abundance and phenotypic differences between individuals. Here, we gathered evidence from recent studies covering several layers that define the genotype-phenotype gap such as: mRNA abundance, allele-specific expression and translation efficiency to demonstrate how genetic variants co-evolve and define an individual's genome...
September 26, 2016: Yeast
https://www.readbyqxmd.com/read/27654890/use-of-ade1-and-ade2-mutations-for-development-of-a-versatile-red-white-colour-assay-of-amyloid-induced-oxidative-stress-in-saccharomyces-cerevisiae
#16
Vidhya Bharathi, Amandeep Girdhar, Archana Prasad, Meenkshi Verma, Vibha Taneja, Basant K Patel
Mutations in adenine biosynthesis pathway genes ADE1 and ADE2 have been conventionally used to score for prion [PSI(+) ] in yeast. If ade1-14 mutant allele is present, which contains a premature stop codon, [psi(-) ] yeast appear red on YPD medium owing to accumulation of a red intermediate compound in vacuoles. In [PSI(+) ] yeast, partial inactivation of the translation termination factor, Sup35 protein, owing to its amyloid aggregation allows for read-through of the ade1-14 stop codon and the yeast appears white as the red intermediate pigment is not accumulated...
September 22, 2016: Yeast
https://www.readbyqxmd.com/read/27599462/metschnikowia-half-tetrads-a-regicide-and-the-fountain-of-youth
#17
Marc-André Lachance
The purpose of this review is to introduce Metschnikowia to the yeast researcher community and to convince readers that the genus is a worthwhile object of study in developmental biology, genetics, ecology and biotechnology. Metschnikowia sits at the foundation of modern immunology, having been instrumental in the discovery of animal phagocytosis. Some 81 species form a monophyletic group within the Metschnikowiaceae, which also include the smaller genus Clavispora and a few clades of Candida species. The family stands out by the habit of forming, by meiosis, only two ascospores, which in Metschnikowia are needle shaped...
September 7, 2016: Yeast
https://www.readbyqxmd.com/read/27168121/discovery-of-genes-involved-in-mitosis-cell-division-cell-wall-integrity-and-chromosome-segregation-through-construction-of-schizosaccharomyces-pombe-deletion-strains
#18
Jun-Song Chen, Janel R Beckley, Liping Ren, Anna Feoktistova, Michael A Jensen, Nicholas Rhind, Kathleen L Gould
The fission yeast model system Schizosaccharomyces pombe is used to study fundamental biological processes. To continue to fill gaps in the Sz. pombe gene deletion collection, we constructed a set of 90 haploid gene deletion strains covering many previously uncharacterized genes. To begin to understand the function of these genes, we exposed this collection of strains to a battery of stress conditions. Using this information in combination with microscopy, proteomics and mini-chromosome loss assays, we identified genes involved in cell wall integrity, cytokinesis, chromosome segregation and DNA metabolism...
September 2016: Yeast
https://www.readbyqxmd.com/read/27145443/insights-from-synthetic-yeasts
#19
Damien Coudreuse
Synthetic biology is one of the most exciting strategies for the investigation of living organisms and lies at the intersection of biology and engineering. Originally developed in prokaryotes, the idea of deciphering biological phenomena through building artificial genetic circuits and studying their behaviours has rapidly demonstrated its potential in a broad range of fields in the life sciences. From the assembly of synthetic genomes to the generation of novel biological functions, yeast cells have imposed themselves as the most powerful eukaryotic model for this approach...
September 2016: Yeast
https://www.readbyqxmd.com/read/27121326/role-of-aif1-in-regulation-of-cell-death-under-environmental-stress-in-candida-albicans
#20
Feiyang Ma, Yueqi Zhang, Yuzhou Wang, Yajuan Wan, Yunheng Miao, Tianyu Ma, Qilin Yu, Mingchun Li
Apoptosis-inducing factor (AIF) is a conserved flavoprotein localized in the mitochondria, inducing apoptosis after translocation into the nucleus. However, its role in the important fungal pathogen, Candida albicans, remains to be investigated. In this study, we find that the C. albicans AIF protein Aif1, similar to its homologues in other organisms, is localized at the mitochondria and translocated into the nucleus under apoptosis-inducing conditions. Moreover, deletion of AIF1 causes attenuated apoptosis in this pathogen under apoptosis-inducing conditions, such as the treatment of 2 mm H2 O2 , 10 mm acetic acid or 0...
September 2016: Yeast
journal
journal
29300
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"