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Microbial Pathogenesis

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https://www.readbyqxmd.com/read/28428134/use-of-physiological-biomarkers-in-diagnosis-along-with-field-trials-of-different-trypanisidal-drugs-in-camels-of-cholistan-desert
#1
Aneela Zameer Durrani, Zubair Bashir, Khalid Mehmood, Muhammad Avais, Haroon Akbar, Waqas Ahmad, Muhammad Azeem
The point prevalence of trypanosomiasis with different physiological biomarkers along with evaluation of the most responsive trypanosidal drug against trypanosomiasis under field conditions was studied. For this purpose a total of 300 free range camels were selected at different grazing and watering point in Cholistan desert. The study population of camels included 150 clinically suspected camels for trypanosomiasis and 150 healthy camels with normal temperature, pulse and respiration. For therapeutic trials 36 positively diagnosed animals were randomly divided into three experimental groups for therapeutic trials...
April 17, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28428133/epidemiological-study-of-common-diseases-and-their-risk-factors-in-camels-in-south-punjab-pakistan
#2
Aneela Zameer Durrani, Zubair Bashir, Imran Rasheed, Noor-Ul-Ain Sarwar
Bacteriological study of mastitis along with common blood protozoan diseases were studied in dromedary camels in Cholistan, Dera Ismail Khan and Rahim Yar Khan districts in South Punjab, Pakistan. For this purpose 300 camels were sampled randomly at different common grazing and watering point. For study of blood parasites clinically suspected and apparently healthy camels, 150 each, were sampled. An overall prevalence of 15%and 5% was recorded for trypanosomiasis and Anaplasmosis respectively. Trypanosoma evansi was identified with 280 bp product on polymerase chain reaction test...
April 17, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28428132/novel-properties-of-hippophae-rhamnoides-l-twig-and-leaf-extracts-anti-virulence-action-and-synergy-with-antifungals-studied-in-vitro-on-candida-spp-model
#3
Beata Sadowska, Aleksandra Budzyńska, Anna Stochmal, Jerzy Żuchowski, Barbara Różalska
Original, chemically characterized Sea buckthorn (SBT) twig and leaf extracts were in vitro studied in terms of anti-Candida activity. Minimum inhibitory concentrations (MICs) of the extracts against C. albicans ATCC 10231 ranged: 250 μg/ml (twig), 31.5 μg/ml (leaf), and against C. glabrata G1 (clinical isolate) - 15.6 μg/ml (twig), 3.9 μg/ml (leaf). Next the extracts have been used at their subMIC. Both extracts significantly enhanced activity of fluconazole (FLC) and caspofungin (CAS) against C. albicans and increased their efficacy against C...
April 17, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28416383/comparative-characterization-analysis-of-synonymous-codon-usage-bias-in-classical-swine-fever-virus
#4
Xin Xu, Dongliang Fei, Huansheng Han, Honggui Liu, Jiayong Zhang, Yulong Zhou, Chuang Xu, Hongbin Wang, Hongwei Cao, Hua Zhang
Classical swine fever virus (CSFV) is responsible for the highly contagious viral disease of swine, and causes great economic loss in the swine-raising industry. Considering the significance of CSFV, a systemic analysis was performed to study its codon usage patterns. In this study, using the complete genome sequences of 76 CSFV representing three genotypes, we firstly analyzed the relative nucleotide composition, effective number of codon (ENC) and synonymous codon usage in CSFV genomes. The results showed that CSFV is GC-moderate genome and the third-ended codons are not preferentially used...
April 14, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28416382/isolation-pathogenicity-and-characterization-of-a-novel-bacterial-pathogen-streptococcus-uberis-from-diseased-mandarin-fish-siniperca-chuatsi
#5
Xia Luo, Xiaozhe Fu, Guoli Liao, Ouqin Chang, Zhibin Huang, Ningqiu Li
In recent years, mandarin fish had a high mortality rate associated with abnormal swimming, exophthalmia, corneal opacity and eye hemorrhage on a fish farm located at Foshan city, Guangdong province, China. Three isolates of Gram-positive, chain-forming cocci were recovered from moribund fish, and designated as SS131025-1, SS131025-2, and SS131025-3. These isolates were identified as Streptococcus uberis according to their morphologic and physio-biochemical characteristics as well as phylogenetic analysis based on their 16S rRNA and GapC gene sequences...
April 14, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28416381/potent-antibacterial-activity-of-biogenic-silver-nanoparticles-synthesized-from-cassia-fistula-fruit
#6
Muhammad Imtiaz Rashid, Liyakat Hamid Mujawar, Mohammad Ibrahim Mujallid, Muhammad Shahid, Zulfiqar Ahmad Rehan, Muhammad Kashif Iqbal Khan, Iqbal M I Ismail
We demonstrated one-step synthesis of silver nanoparticles (AgNPs) from Cassia fistula fruit extract and their antibacterial activity against E. coli and K. pneumoniae. Biogenic AgNPs were characterized by scanning electron microscopy; X-Ray diffraction and fourier transform infrared spectroscopy. Results confirmed spherical shaped AgNPs with an average crystallite size of ∼69 nm. Dose-dependent (0, 10, 20, 40 and 80 μg mL(-1)) growth kinetic studies showed 100% potency against E. coli (20 μg mL(-1)) and K...
April 14, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28414167/inhibition-of-the-mitochondrial-respiratory-chain-in-gills-of-rhamdia-quelen-experimentally-infected-by-pseudomonas-aeruginosa-interplay-with-reactive-oxygen-species
#7
Matheus D Baldissera, Carine F Souza, Mateus Grings, Belisa S Parmeggiani, Guilhian Leipnitz, Karen L S Moreira, Maria Izabel U M da Rocha, Marcelo L da Veiga, Roberto C V Santos, Lenita M Stefani, Bernardo Baldisserotto
It has long been recognized that there are several infectious diseases linked to the impairment of enzymatic complexes of the mitochondrial respiratory chain, with consequent production of reactive oxygen species (ROS), that contribute to disease pathogenesis. In this study, we determined whether the inhibition on mitochondrial respiratory chain might be considered a pathway involved in the production of ROS in gills of Rhamdia quelen experimentally infected by P. aeruginosa. The animals were divided into two groups with six fish each: uninfected (the negative control group) and infected (the positive control group)...
April 13, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28414166/evaluation-and-application-of-molecular-genotyping-on-nosocomial-pathogen-methicillin-resistant-staphylococcus-aureus-isolates-in-guangzhou-representative-of-southern-china
#8
Jian Miao, Lequn Chen, Jingwen Wang, Wenxin Wang, Dingqiang Chen, Lin Li, Bing Li, Yang Deng, Zhenbo Xu
Staphylococcus aureus is recognized as a typical food-born pathogen in food safety, and its rapid detection and typing methods are in desperate need. To study their evolution characteristics and drug resistance condition, Staphylococci isolates were subject to the investigation. Strain identification were subjected to standard procedures (colony morphology, Gram staining, Vitek 2 automated system and the API commercial kit), and fingerprinting was then performed with RAPD-PCR amplification. In this study, 179 isolated staphylococci were identified as MRSA...
April 13, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28412202/the-immunosuppressive-effects-of-a-novel-recombinant-lipq-rv2485c-protein-of-mycobacterium-tuberculosis-on-human-macrophage-cell-lines
#9
Anjani Kumar, Manisha, Gurkamaljit Kaur Sangha, Anju Shrivastava, Jagdeep Kaur
Mycobacterium tuberculosis (MTB), an intracellular pathogen, still represents a major global health challenge. A number of mycobacterial macromolecules have been shown to target biological processes within host macrophages; however, the exact mechanism for the majority of these host pathogen interactions is still poorly understood. Moreover, the lipid metabolic pathway is one of the most important physiologic pathways that plays a vital role in the survival and infection of Mycobacterium tuberculosis. In present study, we investigated the effect of rLipQ from Mycobacterium tuberculosis H37Rv on macrophage functions in vitro...
April 12, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28412201/nitric-oxide-enhanced-the-growth-of-an-obligated-intracellular-bacterium-orientia-tsutsugamushi-in-murine-macrophages
#10
Motohiko Ogawa, Masaaki Satoh, Michiyo Kataoka, Shuji Ando, Masayuki Saijo
Orientia tsutsugamushi is the causative agent of scrub typhus. It is an obligate intracellular bacterium that grows only in eukaryotic cells. Macrophages play an important role in innate immunity by surveilling the human body for pathogens. In present study, it was demonstrated that O. tsutsugamushi propagated well in LPS-activated RAW 264.7 macrophages, but not in non-activated macrophages. In LPS-activated macrophages, the expression of Nos2, which encodes the inducible nitric oxide (NO) synthase (iNOS), was highly upregulated compared to those in non-activated macrophages...
April 12, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28411060/in%C3%A2-vitro-anti-candida-activity-of-selective-serotonin-reuptake-inhibitors-against-fluconazole-resistant-strains-and-their-activity-against-biofilm-forming-isolates
#11
Rose Anny Costa Silva, Cecília Rocha da Silva, João Batista de Andrade Neto, Anderson Ramos da Silva, Rosana Sousa Campos, Letícia Serpa Sampaio, Francisca Bruna Stefany Aires do Nascimento, Brenda da Silva Gaspar, Said Gonçalves da Cruz Fonseca, Maria Aparecida Alexandre Josino, Thalles Barbosa Grangeiro, Danielle Macedo Gaspar, David Freitas de Lucena, Manoel Odorico de Moraes, Bruno Coêlho Cavalcanti, Hélio Vitoriano Nobre Júnior
Recent research has shown broad antifungal activity of the classic antidepressants selective serotonin reuptake inhibitors (SSRIs). This fact, combined with the increased cross-resistance frequency of the genre Candida regarding the main treatment today, fluconazole, requires the development of novel therapeutic strategies. In that context, this study aimed to assess the antifungal potential of fluoxetine, sertraline, and paroxetine against fluconazole-resistant Candida spp. planktonic cells, as well as to assess the mechanism of action and the viability of biofilms treated with fluoxetine...
April 12, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28411059/antibacterial-efficacy-of-silver-nanoparticles-against-multi-drug-resistant-clinical-isolates-from-post-surgical-wound-infections
#12
Muthupandi Kasithevar, Prakash Periakaruppan, Saravanan Muthupandian, Mahalakshmi Mohan
In order to investigate new effective and inexpensive nano-therapeutic approach for P. aeruginosa, staphylococcus aureus and coagulase negative staphylococci (CoNS), the present study reports an eco-friendly process for rapid synthesis of silver nanoparticles (Ag-NPs) using aqueous leaf extract of Corchorus Capsularis (CRCP). Formation of stable Ag-NPs at different time intervals gives mostly spherical particles with diameters ranging from 5 to 45 nm. The resulting Ag-NPs were characterized using Ultraviolet visible (UV-Vis) spectroscopy, Fourier Transform Infrared (FT-IR) spectroscopy, X-Ray Diffraction (XRD) analysis, Transmission Electron Microscopy (TEM) and Energy Dispersive X-ray analysis (EDX)...
April 11, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28400130/salmonella-typhimurium-induces-cloacitis-like-symptomsin-zebrafish-larvae
#13
Macarena Varas, Javiera Ortíz-Severín, Andrés E Marcoleta, Francisco Díaz-Pascual, Miguel L Allende, Carlos A Santiviago, Francisco P Chávez
Pathogenic Salmonella strains have a set of virulence factors allowing them to generate systemic infections and damage in a variety of hosts. Among these factors, bacterial proteins secreted by specialized systems are used to penetrate the host's intestinal mucosa, through the invasion and destruction of specialized epithelial M cells in the intestine. On the other hand, numerous studies have demonstrated that humans, as well as experimental animal hosts, respond to Salmonella infection by activating both innate and adaptive immune responses...
April 8, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28396240/identification-of-proteins-involved-in-the-adhesionof-candida-species-to-different-medical-devices
#14
Arianna Núñez-Beltrán, Everardo López-Romero, Mayra Cuéllar-Cruz
Adhesion is the first step for Candida species to form biofilms on medical devices implanted in the human host. Both the physicochemical nature of the biomaterial and cell wall proteins (CWP) of the pathogen play a determinant role in the process. While it is true that some CWP have been identified in vitro, little is known about the CWP of pathogenic species of Candida involved in adhesion. On this background, we considered it important to investigate the potential role of CWP of C. albicans, C. glabrata, C...
April 7, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28392413/isolation-and-identification-of-multiple-drug-resistant-nontuberculous-mycobacteria-from-organs-of-cattle-produced-typical-granuloma-lesions
#15
Guangyu Cheng, Donglei Xu, Jinling Wang, Chunfa Liu, Yang Zhou, Yongyong Cui, Hancan Liu, Kanglin Wan, Xiangmei Zhou
Nontuberculosis mycobacteria are widespread in the environment and some are zoonotic. 320 tissue samples with visible lesions were obtained from dairy cows and examined by histopathology. Eleven samples showed typical granulomatous lesions and a total of 8 strains were cultured. Three genes (16S rRNA, hsp65 and rpoB) were sequenced for species identification. All mycobacterial isolates were tested for rifampicin, isoniazid, ethambutol, streptomycin, capreomycin, kanamycin, para-aminosalicylic acid susceptibility...
April 6, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28392412/detection-on-integron-carried-gene-cassettes-from-pathogens-by-loop-mediated-isothermal-amplification-assays
#16
Guangchao Yu, Congxiu Ye, Qiang Fu, Juzhen Liu, Xiaoyi Fan, Yunzu Huang, Shishui Zhou
In this study, a number of frequently detected gene cassettes from bacterial integrons have been detected and characterized by rapid and simple loop-mediated isothermal amplification (LAMP) assays. Six gene cassettes commonly found in class 1 integrons were studied, including dfrA12, dfrA17, aadA2, aadA5, orfF, and blaVIM2. Primers design, sensitivity, specificity, optimization of each LAMP assay, as well as application of the developed 6 individual LAMP assays on a large scale of bacteria, had been conducted...
April 6, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28390979/urinary-tract-infections-among-kidney-transplant-patients-due-to-extended-spectrum-beta-lactamase-producing-bacteria
#17
Masoomeh Kashef Nejad, Nima Hosseini Jazani, Yaeghob Sharifi
No abstract text is available yet for this article.
April 5, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28390978/study-on-the-excision-and-integration-mediated-by-class-1-integron-in-enterococcus-faecalis
#18
Guangchao Yu, Congxiu Ye, Qiang Fu, Juzhen Liu, Xiaoyi Fan, Yunzu Huang, Shishui Zhou
Recognized as a mobile genetic element, integron is correlated to the excision and integration of exogenous genes, especially bacterial resistance genes. However, most of the investigations focused on Gram-positive bacteria with few exceptions. In this study, Enterococcus faecalis was selected to investigate the excision and integration of class 1 integron. A total of eight plasmids were subjected to establish the transformants for excision and integration test. As results showed, positive excision assay was observed, which had been confirmed by the further integration assays and PCR amplification...
April 5, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28390977/helicobacter-pylori-genotypes-determine-risk-of-non-cardia-gastric-cancer-and-intestinal-or-diffuse-type-gc-in-ardabil-a-very-high-risk-area-in-northwestern-iran
#19
Esmat Abdi, Saeid Latifi-Navid, Saber Zahri, Abbas Yazdanbod, Reza Safaralizadeh
Frequency of the Helicobacter pylori vacA gene polymorphism and its association with gastric cancer (GC) was assessed in Ardabil, a very high-risk area in Northwestern Iran. We determined the presence of the H. pylori 16S rDNA gene and the vacA s-, m-, i-, and d-region genotypes in DNA from fresh gastric biopsies. Patients with GC were classified based on both the anatomic site and the histopathologic type of tumor Of 135 patients, including 57 with non-atrophic gastritis (NAG) and 78 with GC, 103 were infected by H...
April 5, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28390976/rough-brucella-strain-rm57-is-attenuated-and-confers-protection-against-brucella-melitensis
#20
Yu Feng, Xiaowei Peng, Hui Jiang, Yong Peng, Liangquan Zhu, Jiabo Ding
Brucella melitensis (B. melitensis) is a facultative intracellular pathogen, which is the main epidemic strain in China. To overcome disadvantages of traditional live attenuated vaccines, in this study a rough mutant RM57 was induced from a B. melitensis isolate M1981. In order to uncover the reason of changes in the LPS of RM57, the nucleotide sequences and transcription levels of all known genes related to LPS synthesis were detected. As LPS plays an important role in outer membrane integrity, the sensitivities of RM57 to SDS and polymyxin B were examined...
April 5, 2017: Microbial Pathogenesis
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