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Microbial Pathogenesis

U Jinu, M Gomathi, I Saiqa, N Geetha, G Benelli, P Venkatachalam
This research focused on green engineering and characterization of silver (PcAgNPs) and copper nanoparticles (PcCuNPs) using Prosopis cineraria (Pc) leaf extract prepared by using microwave irradiation. We studied their enhanced antimicrobial activity on human pathogens as well as cytotoxicity on breast cancer cells (MCF-7). Biofabricated silver and copper nanoparticles exhibited UV-Visible absorbance peaks at 420 nm and 575 nm, confirming the bioreduction and stabilization of nanoparticles. Nanoparticles were characterized by FTIR, XRD, FESEM, and EDX analysis...
February 15, 2017: Microbial Pathogenesis
Rima Tapader, Dipro Bose, Amit Pal
YghJ, also known as SsLE (Secreted and surface associated lipoprotein) is a cell surface associated and secreted lipoprotein harbouring M60 metalloprotease domain. Though the gene is known to be conserved among both pathogenic and commensal E. coli isolates, the expression and secretion of YghJ was found to be higher among diverse Escherichia coli pathotypes. YghJ, secreted from intestinal pathogens such as enterotoxigenic E. coli (ETEC) and enteropathogenic E. coli (EPEC) has been demonstrated to possess mucinase activity and hence facilitates colonization of these enteric pathogens to intestinal epithelial cells...
February 14, 2017: Microbial Pathogenesis
Fatima Ayoub, Najwa Ben Oujji, Bouchra Chebli, Mohamed Ayoub, Athman Hafidi, Rachid Salghi, Shehdeh Jodeh
In the attempt to reduce the negative impacts of chemical pesticides on environment and consumer's health, a new plant treatment practice minimizing the amount of pesticides needed during pests and diseases treatments has been developed. Our approach is based on combining the biocide effects of fungicide with the peroxyacetic acid (PAA) one. In this paper, we focused on the in vitro study of the antifungal activity of this combination against Botrytis cinerea, the most redoubtable threat of tomatoes plants in Morocco...
February 10, 2017: Microbial Pathogenesis
Kirubakaran Rangasamy, Murugan Athiappan, Natarajan Devarajan, Javid A Parray
Impacts of pesticide exposure on the soil microbial flora and cross resistance to antibiotics have not been well documented. Development of antibiotic resistance is a common issue among soil bacteria which are exposing to pesticides continuously at sub-lethal concentration. The present study was focused to evaluate the correlation between pesticide exposures and evolution of multi drug resistance among isolates collected from soil applied with insecticides. Twenty five insecticide (Monochrotophos) degrading bacteria were isolated from contaminated agricultural soil...
February 9, 2017: Microbial Pathogenesis
K K Liyanage, Sehroon Khan, Siraprapa Brooks, Peter E Mortimer, Samantha C Karunarathna, Jianchu Xu, Kevin D Hyde
Powdery mildew is a fungal disease that infects a wide range of plants, including rubber trees, which results in a reduction of latex yields of up to 45%. The causal agent of powdery mildew of rubber was first described as Oidium heveae, but later morpho-molecular research suggested that in the past, O. heveae has been confused with Erysiphe quercicola. However, it is still under debate whether the causal agent should be classified as a species of the genus Erysiphe emend. or Golovinomyces and Podosphaera, respectively...
February 9, 2017: Microbial Pathogenesis
Mohmmad Younus Wani, Aijaz Ahmad, Santosh Kumar, Abilio J F N Sobral
Invasive fungal infection is a problem that continues to challenge the healthcare sector. New antifungals and new therapeutic strategies are needed to address this challenge. We previously reported that the combination of a synthetic compound with a drug with known mechanism of action is a good strategy to treat aggressive and resistant fungi. Here we revisited our approach and synthesized structural analogues of flucytosine, which is a synthetic antifungal and is being studied for its use in combination therapy with other antifungal drugs...
February 9, 2017: Microbial Pathogenesis
Zhi-Qiang Zou, Shuai Zhang, Qing Lin, Ren-Liang Qu, Yan-Fang Li, Fu-Hua Zhang, Ai-Ling Xu
BACKGROUND: Chronic hepatitis B (CHB) is a complicated and dynamic course, and is associated with advanced liver disease. Host immune response against viral infection plays a pivotal role in the progression of CHB. However, it is still uncharted that how the hepatic transcriptomes in patients with CHB are correlated with the clinical phases. OBJECTIVE: This study aimed to identify the specific sub-networks across various phases of CHB and infer potential pathways for phenotypic outcome prediction...
February 9, 2017: Microbial Pathogenesis
Zhong-Rui Bian, Juan Yin, Wen Sun, Dian-Jie Lin
Diagnose of active tuberculosis (TB) is challenging and treatment response is also difficult to efficiently monitor. The aim of this study was to use an integrated analysis of microarray and network-based method to the samples from publically available datasets to obtain a diagnostic module set and pathways in active TB. Towards this goal, background protein-protein interactions (PPI) network was generated based on global PPI information and gene expression data, following by identification of differential expression network (DEN) from the background PPI network...
February 8, 2017: Microbial Pathogenesis
Behrooz Alizadeh Behbahani, Farideh Tabatabaei Yazdi, Fakhri Shahidi, Seyed Ali Mortazavi, Mohebbat Mohebbi
Principle component analysis (PCA) was employed to examine the effect of the exerted treatments on the beef shelf life as well as discovering the correlations between the studied responses. Considering the variability of the dimensions of the responses, correlation coefficients were applied to form the matrix and extract the eigenvalue. Antimicrobial effect was evaluated on 10 pathogenic microorganisms through the methods of hole-plate diffusion method, disk diffusion method, pour plate method, minimum inhibitory concentration and minimum bactericidal/fungicidal concentration...
February 8, 2017: Microbial Pathogenesis
Michel Drancourt, Gérard Aboudharam, Olivier Croce, Fabrice Armougom, Catherine Robert, Didier Raoult
The in-laboratory contamination of the ancient samples hinders the result interpretation of the investigations in the field of paleomicrobiology. We had promoted the dental pulp as a sample that limits the risks of in-laboratory contamination of the ancient material. In this work, we measured the contamination of the dental pulp manipulated according to paleomicrobiology protocol, used as a source of a total DNA for metagenomics. First, total DNA extracted from two dog canines was sequenced using next generation sequencing...
February 8, 2017: Microbial Pathogenesis
Dexian Zhang, Jingcui Zhao, Qiuxia Wang, Yaochuan Liu, Chunlian Tian, Yujun Zhao, Lihui Yu, Mingchun Liu
Trueperella pyogenes plays a crucial role in endometritis pathogenesis and is also associated with many infections, including metritis, mastitis, arthritis and liver abscessation, in many domestic animals. In this study, we investigated the prevalence of tetracycline resistance in T. pyogenes isolated from dairy cows with endometritis in Inner Mongolia, China, and we assessed tetracycline-resistance gene distribution among the isolates. Our results indicated that 68.7% and 62.5% of the isolates were resistant to tetracycline and doxycycline, respectively, and the rate of resistance to metacycline was 18...
February 8, 2017: Microbial Pathogenesis
Amin Tashakor, Mahshid Hosseinzadehdehkordi, Zeynab Emruzi, Dariush Gholami
In the present study, we isolated Lactobacillus sakei strain DGH5 from raw beef meat. This bacterium plays an inhibitory effect against food-spoiling bacteria and food-borne pathogens, including Listeria monocytogenes, a gram-positive and pathogenic bacterium. Lactobacillus sakei strain DGH5 was identified through both phenotypical and biochemical tests accompanied with 16S rRNA sequence analysis. Among all the sources of carbon, nitrogen and phosphorous forms, we selected the most potent compounds to optimize the condition for the highest antagonistic activity...
February 8, 2017: Microbial Pathogenesis
Reghunathan S Preethanath, Nadia W AlNahas, Sahar M Bin Huraib, Hana O Al-Balbeesi, Naif Khalid Almalik, M H N Dalati, Darshan Devang Divakar
Although dental implants are most common prosthetic treatment used to replace missing tooth, it gained considerable importance over a decade owing to the availability of advanced imagery techniques that can help in achieving a greater success rate and much better osseointegration. However, the chances that the implanted tooth can be rejected due to inflammation caused by oral microflora still persist. This review gives the viewers an overall idea of the dental implants, role of advanced imaging in implantation and instances of peri-implantitis that occur after implantation process...
February 7, 2017: Microbial Pathogenesis
Carine F Souza, Matheus D Baldissera, Luciana F Cossetin, Daiane F Dalla Lana, Silvia G Monteiro
Oxidative stress has been considered as a pathological mechanism that contributes to initiation and progression of cardiac injury during the Trypanosoma evansi infection. In this sense, the natural compounds with antioxidant and free radical scavenger abilities, such the Achyrocline satureioides essential oil loaded in nanocapsules (AS-NC), may be considered important approach to minimize the cardiac damage. Thus, the aim of this study was to investigate whether AS-NC treatment is able to prevents or reduce the cardiac oxidative damage in infected rats with T...
February 6, 2017: Microbial Pathogenesis
Mehdi Goudarzi, Mahnaz Bahramian, Mahboobeh Satarzadeh Tabrizi, Edet E Udo, Agnes Marie Sá Figueiredo, Maryam Fazeli, Hossein Goudarzi
Methicillin-resistant Staphylococcus aureus (MRSA) as a major cause of infection in health care, hospital and community settings is a global health concern. The purpose of this study was to determine the antibiotic susceptibility pattern and distribution of circulating molecular types of MRSA in a burn hospital in Tehran, the capital of Iran. During a 10-month study period, 106 Staphylococcus aureus isolates were assessed. Isolates were subjected to susceptibility testing using the disk diffusion method and Polymerase Chain Reaction (PCR) for detection of mecA, fem and nuc genes...
February 5, 2017: Microbial Pathogenesis
Chandrika Murugaiah, Nik Zuraina Nik Mohd Noor, Shyamoli Mustafa, Ravichandran Manickam, Lalitha Pattabhiraman
Cholera, a severe form of gastroenteritis, is one of the most widespread diseases in developing countries. The mechanism of intestinal infection caused by V. cholerae O139 remains unclear. In order to explore some morphological aspects of its infection in the intestine including Peyer's patches, we investigated the V. cholerae O139 infection at intestinal site of the rabbit gut-loop model. The electron microscopic analysis revealed denuded mucosal surface with loss of microvilli and integrity of the surface epithelium...
February 4, 2017: Microbial Pathogenesis
Baifen Song, Xijing Yang, Hunan Sun, Liquan Yu, Jinzhu Ma, Zhijun Wu, Yudong Cui
Streptococcus is one of the main pathogens that cause bovine mastitis. They includes into S.agalactiae, S.dysgalactiae, and S.uberis. The GapC protein is a virulence factor that is expressed on the surface of Streptococcus species. GapC is highly antigenic and immunization with GapC confers cross-protection against all three species. Our previous data showed that amino acids 1-150 of GapC (GapC1-150) of S. dysgalactiae conferred similar immunoprotection compared to full-length GapC. Thus, the present study aimed to construct a recombinant Escherichia coli XL1-Blue strain that displayed GapC1-150 on its surface, and to investigate the immunogenicity of the surface-localized GapC1-150...
February 4, 2017: Microbial Pathogenesis
Muxia Yan, Ling Xu, Hua Jiang, Zhenwen Zhou, Shishui Zhou, Li Zhang
In exposure to outer pressure, microorganisms are capable of entry into the Viable But Non-Culturable (VBNC) state, and thus survive under various elimination processing. The survival microorganisms may yield negative results on culturing, and cause false negative for this golden standard methodology. In this study, a novel PMA-LAMP assay on the detection of Enterohemorrhage E. coli and shiga toxins has been developed and evaluated, with further application on a number of food borne E. coli strains. LAMP primers were designed on the target of rfbe for Enterohemorrhage E...
February 3, 2017: Microbial Pathogenesis
P A Desingu, S D Singh, K Dhama, O R Vinodh Kumar, Y S Malik, R Singh
Newcastle disease (ND) is an economically important viral disease distressing poultry industry across the globe. Herein, we report the clinicopathology of sub-genotype VIIi Newcastle disease virus (NDV) isolated from peafowl in chickens. The virus isolate produced systemic infection with prominent tropism in visceral organs in chicken, confirmed on the basis of gross and microscopic lesions, and immunohistochemistry findings. The experimentally infected chickens exhibited 100% mortality with severe hemorrhagic lesions in the proventriculus and intestine, especially marked lymphocytolysis in spleen and bursa...
February 3, 2017: Microbial Pathogenesis
Ruqin Lin, Bo Zhu, Yiduo Zhang, Yang Bai, Fachao Zhi, Beiguo Long, Yawen Li, Yuhua Wu, Xianbo Wu, Hongying Fan
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 causes hemorrhagic colitis and hemolytic uremic syndrome in humans. Due to the risks associated with antibiotic treatment against EHEC O157:H7 infection, vaccines represent a promising method for prevention of EHEC O157:H7 infection. Therefore, we constructed the novel bivalent antigen EspA-Tir-M as a candidate EHEC O157:H7 subunit vaccine. We then evaluated the immunogenicity of this novel EHEC O157:H7 subunit vaccine. Immune responses to the fusion protein administered by intranasal and subcutaneous routes were compared in mice...
February 2, 2017: Microbial Pathogenesis
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