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Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire

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https://www.readbyqxmd.com/read/28746817/human-bone-marrow-mesenchymal-stem-cells-bmscs-suppress-hepatic-stellate-cells-hscs-proliferation-by-inhibiting-the-ubiquitylation-of-p27
#1
Liang Wang, Guang Bai, Fei Chen
Bone marrow mesenchymal stem cells (BMSCs) have considerable therapeutic potential for the treatment of end-stage liver disease. Previous studies have demonstrated that BMSCs secreted growth factors and cytokines to inactivate hepatic stellate cells (HSCs), which inhibited the progression of hepatic fibrosis. The aim of this study was to determine the mechanism by which BMSCs suppress the function of HSCs in fibrosis. Our results showed that co-culture of BMSCs and HSCs induced cell cycle arrest at the G10/G1 phase and cell apoptosis of HSCs, which finally inhibited the cell proliferation of HSCs...
July 26, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28746809/fetal-alcohol-spectrum-disorder-diagnosis-current-practices-and-future-considerations
#2
Albert Chudley
This paper discusses the current state of knowledge and practice of Fetal Alcohol Spectrum Disorder (FASD) diagnosis. The strengths and challenges of different models of diagnosis are compared. Some models require a team approach for evaluation, while other approaches assume that a clinician in his or her office provide a diagnosis based on a review of the patient's medical and social history, behaviour and physical examination. The author reviews the emergence of new information from recent advances in genetics, imaging and electrophysiology that have potential to lead to changes in practice and improved reliability of an FASD diagnosis...
July 26, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28708947/kv10-1-potassium-channel-from-the-brain-to-the-tumors
#3
Verna Cázares-Ordoñez, Luis A Pardo
<i>KCNH1</i> gene encodes the Kv10.1 (Eag1) ion channel, a member of the EAG (ether-à-go-go) family of voltage-gated potassium channels. Recent studies have demonstrated that <i>KCHN1</i> mutations are implicated in Temple-Baraitser and Zimmermann-Laband syndromes and other forms of developmental deficits that all present with mental retardation and epilepsy, suggesting that Kv10.1 might be important for cognitive development in human. Although Kv10.1 channel is mainly expressed in the mammalian brain, its ectopic expression occurs in 70% of human cancers...
July 14, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28708941/regulation-of-the-insulin-akt-signaling-pathway-and-glycolysis-during-dehydration-stress-in-the-african-clawed-frog-xenopus-laevis
#4
Cheng-Wei Wu, Shannon N Tessier, Kenneth B Storey
Estivation is an adaptive stress response utilized by some amphibians during periods of drought in the summer season. In this study, we examine the regulation of the insulin signaling cascade and glycolysis pathway in the African clawed frog Xenopus laevis during dehydration stress induced state of estivation. We show that in the brain and heart of X. laevis, dehydration reduces the phosphorylation of the insulin growth factor-1 receptor (IGF-1R), and this is followed by similar reductions in the phosphorylation of the Akt and mechanistic target of rapamycin (mTOR) kinase...
July 14, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28700834/petroleum-ether-extract-of-njavara-rice-bran-upregulates-jak2-stat3-mediated-anti-inflammatory-profile-in-macrophages-and-aortic-endothelial-cells-promoting-regression-of-atherosclerosis
#5
Chithra K Pushpan, Jayalekshmy Ananthasankaran, Helen Antony
"Njavara"(Oryza sativa Linn) is a unique rice variety grown in Kerala that is reported to have significantly higher antioxidant, anti-inflammatory, chemical indices, and bioactive components compared with staple rice varieties. However, the role of NBE in reversing the atherosclerosis development remains unclear. The present study aimed to elucidate the role of Njavara rice bran petroleum ether extract (NBE) in promoting atherosclerotic regression. Male New Zealand white breed rabbits were divided into three groups...
July 12, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28686845/metabolomics-and-fetal-alcohol-spectrum-disorder-a-mini-review
#6
Erin Goldberg, Michel Aliani
Fetal alcohol spectrum disorder (FASD) is a major public health issue that encompasses an array of physical, neurological and behavioral effects due to alcohol consumption during pregnancy. Classical biomarkers of FASD that are currently used lack sensitivity and specificity, and as such there is an opportunity through the use of novel metabolomics analysis to identify novel biomarkers to identify those at risk for FASD, which could more effectively aid in early intervention. The focus of this mini-review is to identify current work that has been done in the field of metabolomics in FASD in utero, and to highlight promising metabolites that could act as biomarkers in the future...
July 7, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28662337/danshen-attenuates-osteoarthritis-related-cartilage-degeneration-through-inhibition-of-nf-%C3%AE%C2%BAb-signaling-pathway-in-vivo-and-in-vitro
#7
Xilin Xu, Hang Lv, Xiaodong Li, Hui Su, Xiaofeng Zhang, Jun Yang
Danshen is a traditional Chinese medicine herb that can alleviate osteoarthritis (Soder et al.) symptoms in animals. However, the underlying mechanisms remain poorly understood and require further investigation. In this study, rabbits with experimental OA were given intra-articular injection of Danshen (0.7 mL/day) for five weeks. In addition to attenuating the cartilage degeneration of OA rabbits, Danshen decreased the expression and activity of matrix metalloproteinase 9 (MMP-9) and MMP-13, whereas increased that of their natural inhibitors, tissue inhibitor of matrix metalloproteinase 1 (TIMP-1) and TIMP-2...
June 29, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28658581/modulation-of-procaspase-7-self-activation-by-pest-amino-acid-residues-of-the-n-terminal-prodomain-and-intersubunit-linker
#8
Juliano Alves, Humberto Miguel Garay-Malpartida, João Marcelo Occhiucci, Jose Belizario
Procaspase-7 zymogen polypeptide is formed by a short prodomain, a large subunit (p20) and a small subunit (p10) connected to an intersubunit linker. Caspase-7 is activated by an initiator caspase -8 and -9 or by autocatalysis after specific cleavage at IQAD198↓S located at intersubunit linker. Previously, we identified that PEST regions made of amino acid residues Pro (P), Glu (E), Asp (D), Ser (S), Thr (T), Asn (N) and Gln (Q) are conserved flanking amino acid residues in the cleavage sites within at prodomain and intersubunit linker of all caspase family members...
June 28, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28654762/jatropha-6-17-11e-diene-class-derivatives-induce-apoptosis-effects-in-ovcar-3-and-caov-4-ovarian-cancer-cell-lines-via-mitochondrial-pathway
#9
Behzad Bahmani, Mohammad Keyvanloo Shahrestanaki, Mustafa Ghanadian, Sima Hajiahmadi, Mahmoud Aghaei
We investigated the molecular mechanism of apoptosis induced by novel jatropha-6(17),11E-diene class derivatives, compounds A, B, and C extracted from Euphorbia osyridea in Caov-4 and OVCAR-3 ovarian cancer cell lines. OVCAR-3 and Caov-4 cell lines were treated with different concentrations of these compounds. Cytotoxicity was evaluated by MTT, clonogenic survival assay and Flow cytometry assays. ROS production, mitochondrial membrane potential (ΔΨm), Caspase 3 and 9 activities were evaluated. Compound A, B, and C reduced cell viability in a dose-dependent manner (P<0...
June 27, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28595020/tamoxifen-inhibits-mitochondrial-membrane-damage-caused-by-disulfiram
#10
Natalia Pavon, Mabel Buelna-Chontal, Francisco Correa, Belem Yoval-Sánchez, Javier Belmont, Luz Hernández-Esquivel, Jose S Rodríguez-Zavala, Edmundo Chavez
In this work, we studied the protective effect of tamoxifen on disulfiram-induced mitochondrial membrane insult. The results indicate that tamoxifen circumvents inner membrane leakiness manifested by Ca2+ release, mitochondrial swelling, and collapse of the transmembrane electric gradient. Furthermore, it was found that tamoxifen prevents the inactivation of the mitochondrial enzyme aconitase and detachment of cytochrome c from the inner membrane. Interestingly, tamoxifen also inhibited disulfiram-promoted generation of hydrogen peroxide...
June 8, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28544853/microrna-34a-protect-myocardial-cells-against-ischemia-reperfusion-injury-through-inhibiting-autophagy-via-regulating-tnf%C3%AE-expression
#11
Haifeng Shao, Lili Yang, Li Wang, Bozan Tang, Jian Wang, Qiang Li
BACKGROUND: Ischemia/reperfusion (I/R) accompanying with the blood supply restored after myocardial infarction. Myocardial I/R injury relieves when autophagy decreased. MicroRNA-34a(miR-34a) regulate autophagy in renal I/R injury model. But whether it can affect cardiac I/R injury remains studying. This study investigated how miR-34a in protecting myocardial cells against I/R injury through inhibit autophagy via regulating tumor necrosis factor α (TNFα) . METHODS: Constructed the I/R model in vivo with Langendorff; treated Neonatal Rat cardiomyocyte to make H/R injury model in vitro with hypoxia/reoxygenation (H/R) method...
May 25, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28538104/wnt5a-regulates-the-expression-of-ror2-tyrosine-kinase-receptor-in-ovarian-cancer-cells
#12
Ghamartaj Hossein, Somayeh Arabzadeh, Zahra Salehi-Doulabi, Zeynab Dehghani-Ghobadi, Yasaman Heidarian, Maryam Talebi-Juybari
Wnt5A and Receptor tyrosine kinase-like orphan receptor 2 (ROR2) proteins both regulate developmental processes, cell movement, and cell polarity. The purpose of the present study was to evaluate a possible regulatory role of Wnt5A on ROR2 expression in human ovarian cancer cell lines. Moreover, the expression of Wnt5A and ROR2 mRNA and protein levels were assessed in human epithelial serous ovarian cancer (HSOC) specimens. ROR2 was strongly decreased in cells treated with siRNA against Wnt5A compared to scramble-treated or lipofectamine-treated cells (P<0...
May 24, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28525726/characterization-of-an-ionic-liquid-tolerant-%C3%AE-xylosidase-from-a-marine-derived-fungal-endophyte
#13
Anindita Sengupta, Angela Zabala, Si Yu Tan, Arthur Broadstock, T S Suryanarayanan, Venkat Gopalan
Ionic liquids (ILs) are used in lignocellulosic biomass (LCB) pretreatment due to their ability to disrupt the extensive hydrogen-bonding network in cellulose and hemicellulose, and thereby decrease LCB recalcitrance to subsequent enzymatic degradation. However, this approach necessitates development of a cellulases and hemicellulases that can tolerate ~20% (w/v) IL, an amount that either co-precipitates with the sugar polymers after the initial pretreatment or is typically used in single-pot biomass deconstructions...
May 19, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28525724/interaction-between-mir-572-and-ppp2r2c-and-their-effects-on-proliferation-migration-and-invasion-of-nasopharyngeal-carcinoma-npc-cells
#14
Lei Yan, Kerui Cai, Jun Liang, Haifeng Liu, Yang Liu, Jinqiu Gui
We investigated the regulation of miR-572 on PPP2R2C and studied their effects on proliferation, migration as well as invasion of nasopharyngeal carcinoma (NPC) cells. NPC tissues and normal tissues were collected, and the expressions of miR-572 and PPP2R2C were detected by RT-PCR. And Western blot was applied to detect the expression of PPP2R2C protein. The target relationship between miR-572 and PPP2R2C was confirmed by dual luciferase reporter gene assay. MTT assay and flow cytometry was applied to investigate the viability and apoptosis of NPC cells...
May 19, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28521112/prevalence-of-externalizing-disorders-and-autism-spectrum-disorder-among-children-with-fetal-alcohol-spectrum-disorder-systematic-review-and-meta-analysis
#15
Shannon Lange, Jürgen Rehm, Evdokia Anagnostou, Svetlana Popova
Due to their central nervous system impairments, children with Fetal Alcohol Spectrum Disorder (FASD) commonly exhibit externalizing behaviours such as hyperactivity, impulsivity, and/or delinquency. The purpose of the current study was to estimate the prevalence of neurodevelopmental disorders with prominent externalizing behaviours, namely Attention-Deficit Hyperactivity Disorder (ADHD), Conduct Disorder (CD), Oppositional Defiant Disorder (ODD), as well as Autism Spectrum Disorder (ASD) among children with FASD...
May 18, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28521108/mir-301b-promotes-the-proliferation-mobility-and-epithelial-to-mesenchymal-transition-of-bladder-cancer-cells-by-targeting-egr1
#16
Lei Yan, Yan Wang, Jun Liang, Zhixin Liu, Xiaodong Sun, Kerui Cai
We aimed to investigate the role of miR-301b in the modulation of the proliferation, migration and invasion in bladder cancer (BLCA) cells. The expression of miR-301b and EGR1 (early growth response gene 1) mRNA were analyzed by qRT-PCR (quantitative real-time polymerase chain reaction). Dual-luciferase reporter gene system was used to identify the target relationship between miR-301b and EGR1. Cell proliferation, cell cycle and apoptosis were analyzed by MTT assay, colony-forming assay and flow cytometry, respectively...
May 18, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28482162/mir-129-5p-suppresses-gastric-cancer-cell-invasion-and-proliferation-by-inhibiting-col1a1
#17
Quan Wang, Jinhai Yu
In this study, we aimed to explore the role of miR-129-5p, a newly identified miR-129 member, in GC cells as well as the potential mechanism. The results of RT-qPCR and Western Blot showed that miR-129 was downregulated in gastric cancer (GC) cells compared with normal ones. Using MTT, colony formation, wound healing assay and Transwell assay, we evaluated the proliferation, migration and invasion abilities of transfected cells and confirmed miR-129-5p as a tumor suppressor in GC. Measured by RT-qPCR and Western Blot, COL1A1 turned out to be upregulated in GC tissues and cells...
May 8, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28467858/anti-angiogenic-effect-of-chebulagic-acid-involves-inhibition-of-vegfr2-and-gsk3%C3%AE-dependent-signaling-pathways
#18
A P Athira, C S Abhinand, K Saja, A Helen, P Reddanna, Perumana R Sudhakaran
Inhibition of angiogenesis is a useful strategy to prevent cancer growth, which targets new vessels that grow to nourish actively proliferating tumor cells. Endothelial cells can use a number of different pathways to cause angiogenesis and each step in these pathways can be targeted. The use of multi targeted drugs is gaining much importance in this scenario. Our previous results have shown the anti angiogenic effect of Chebulagic acid - a benzopyran tannin present in the fruits of Terminalia chebula. The present study was designed to examine the molecular mechanism of the anti angiogenic effect of chebulagic acid...
May 3, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28460187/tlr2-affects-cd86-expression-and-inflammatory-response-in-burn-injury-mice-through-regulation-of-p38
#19
Li Li, Gang Xu, Chenwang Duan
The aim of this study was to assess the effects of TLR2/p38/CD86 signaling pathways on inflammatory response in a mouse model of burn injury. Wild-type (TLR2+/+) and mutant-type (TLR2-/-) mice were obtained, and a mouse burn injury model was constructed. Tissue samples were detected using HE staining and Transferase Mediated Nick End Labeling (TUNEL) method. Macrophages were treated with TLR2 agonist and p38 inhibitor. The expression levels of TLR2, p38, CD86, IL-1β and TNF-α were identified by RT-qPCR, Western blot and ELISA...
May 1, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28314105/interactions-of-u24-from-roseolovirus-with-ww-domains-canonical-vs-noncanonical
#20
Yurou Sang, Rui Zhang, A Louise Creagh, Charles A Haynes, Suzana K Straus
U24 is a C-terminal membrane-anchored protein found in both human herpes virus type 6 and 7 (HHV-6 and HHV-7), with an N-terminal segment that is rich in prolines (PPxY motif in both HHV-6A and 7; PxxP motif in HHV-6A). Previous work has shown that U24 interacts strongly with Nedd4 WW domains, in particular, hNedd4L-WW3*. It was also shown that this interaction depends strongly on the nature of the amino acids that are upstream from the PY motif in U24. In this contribution, data was obtained from pull-downs, isothermal titration calorimetry, and NMR to further determine what modulates U24:WW domain interactions...
June 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
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