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Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire

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https://www.readbyqxmd.com/read/28178421/lactoferrin-interacts-with-splunc1-to-attenuate-lipopolysaccharide-induced-inflammation-of-human-nasal-epithelial-cells-via-down-regulated-mek1-2-mapk-signaling
#1
Yung-An Tsou, Yu-Tong Tung, Tsu-Fang Wu, Gary Ro-Lin Chang, Han-Chien Chen, Chia-Der Lin, Chih-Ho Lai, Hsiao-Ling Chen, Chuan-Mu Chen
The short palate, lung, and nasal epithelium clone 1 (SPLUNC1) protein is an important innate material in the upper airway, and lactoferrin (LF) aids the innate functions in humans. In this study, an nasal epithelial model was used to investigate how LF modulates SPLUNC1 to reduce the inflammatory process mediated by lipopolysaccharide (LPS). The inflammation of human RPMI-2650 cells was induced by LPS to evaluate SPLUNC1 expression after treating the cells with bovine LF (bLF). The interaction pathway between LF and SPLUNC1 in LPS-induced inflammation was further investigated...
February 8, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177755/modulation-of-amyloid-assembly-by-glycosaminoglycans-from-mechanism-to-biological-significance
#2
Noe Quittot, Mathew Sebastiao, Steve Bourgault
Glycosaminoglycans (GAGs) are long and unbranched polysaccharides that are abundant in the extracellular matrix and basement membrane of multicellular organisms. These linear polyanionic macromolecules are involved in many physiological functions, from cell adhesion to cellular signaling. Interestingly, amyloid fibrils extracted from patients afflicted with protein misfolding diseases are virtually always associated with GAGs. Amyloid fibrils are highly organized nanostructures that have been historically associated with pathological states, such as Alzheimer's disease and systemic amyloidoses...
February 1, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177754/comparison-of-the-effects-of-nobiletin-and-letrozole-on-the-activity-and-expression-of-aromatase-in-mcf-7-breast-cancer-cell-line
#3
Seyedeh Tayebeh Rahideh, Mohammad Keramatipour, Mitra Nourbakhsh, Fariba Koohdani, Mostafa Hoseini, Saeed Talebi, Farzad Shidfar
Nobiletin (NOB) is one of the polymethoxyflavones mainly found in citrus fruits. Aromatase or cytochrome P450 (CYP19) enzyme catalyzes the last and rate-limiting step in estrogen biosynthesis. This study was carried out to investigate the effect of NOB on the activity and expression of aromatase and compare this property with letrozole (LET) as aromatase inhibitor in MCF-7 breast cancer cell line. The cell viability was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assays...
February 1, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177753/histone-h3-3-regulates-mitotic-progression-in-mouse-embryonic-fibroblasts
#4
Aysegul Ors, Christophe Papin, Bertrand Favier, Yohan Roulland, Defne Dalkara, Mehmet Ozturk, ALi Hamiche, Stefan Dimitrov, Kiran Padmanabhan
H3.3 is a histone variant, which marks transcription start sites as well as telomeres and heterochromatic sites on the genome. H3.3 presence is thought to positively correlate with transcriptional status of its target genes. Using a conditional genetic strategy against H3.3B combined with short hairpin RNAs against H3.3A, we essentially depleted all H3.3 gene expression in mouse embryonic fibroblasts. Following nearly complete loss of H3.3 in cells, our transcriptomic analyses show very little impact on global gene expression as well as on histone variant H2A...
February 1, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177756/method-comparison-for-analyzing-wound-healing-rates
#5
Prabhpreet K Dhillon, Xinyin Li, Jurgen T Sanes, Oluwafemi S Akintola, Bingyun Sun
Wound healing scratch assay is a frequently used method to characterize cell migration, which is an important biological process in the course of development, tissue repair, and immune response for example. The measurement of wound healing rate, however, varies among different studies. Here we summarized these measurements into three types: I) Direct Rate Average; II) Regression Rate Average; and III) Average Distance Regression Rate. Using Chinese Hamster Ovary (CHO) cells as a model, we compared the three types of analyses on quantifying the wound closing rate, and discovered that type I & III measurements are more resistant to outliers and type II analysis is more sensitive to outliers...
January 30, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177757/microrna-181-inhibits-proliferation-and-promotes-apoptosis-of-chondrocytes-in-osteoarthritis-by-targeting-pten
#6
Xiao-Feng Wu, Zi-Hui Zhou, Jian Zou
OBJECTIVE: To investigate the effects of microRNA-181 (miR-181) on proliferation and apoptosis of chondrocytes in osteoarthritis (OA) by targeting PTEN. METHODS: The chondrocytes in logarithmic growth phase were selected and divided into 6 groups: the normal, blank, negative control, miR-181 mimics, miR-181 inhibitors and miR-181 inhibitors + PTEN-siRNA groups. RT-qPCR was used to detect the expressions of miR-181 and PTEN mRNA. MTT assay and flow cytometry were performed to detect cell proliferation and apoptosis...
January 25, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177758/differential-effect-of-hypoxia-and-acidity-on-lung-cancer-cell-and-fibroblast-metabolism
#7
Alexandra Giatromanolaki, Maria Liousia, Stella Arelaki, Dimitra Kalamida, Stamatia Pouliliou, Axilleas Mitrakas, Avgi Tsolou, Efthimios Sivridis, Michael I Koukourakis
This study examined the metabolic response of lung cancer cells and normal lung fibroblasts to hypoxia and acidity. GLUT1 and HXKII mRNA/protein expression was up-regulated under hypoxia in the MRC5 fibroblasts and in the A549 and H1299 lung cancer cell lines, indicating intensified glucose absorption and glycolysis. Under hypoxia, the LDHA mRNA and LDH5 protein levels increased in the cancer cells but not in the fibroblasts. Acidity suppressed the above mentioned hypoxia effect. PDH-kinase-1 (PDK1 mRNA and protein) and inactive phosphorylated-PDH protein levels were induced under hypoxia in the cancer cells, whilst these were reduced in the MRC5 lung fibroblasts...
January 24, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177760/ceruloplasmin-derived-peptide-is-the-strongest-regulator-of-oxidative-stress-and-leukotriene-synthesis-in-neutrophils
#8
Ekaterina Golenkina, Alexey Livenskyi, Galina Viryasova, Yulia Romanova, Galina Sud'ina, Alexey Sokolov
Ceruloplasmin, an acute-phase protein, can affect the activity of leukocytes through its various enzymatic activities and protein-protein interactions (with lactoferrin, myeloperoxidase, eosinophil peroxidase, serprocidins, and 5-lipoxygenase (5-LOX), among others). However, the molecular mechanisms of ceruloplasmin activity are not clearly understood. In this study, we tested the ability of two synthetic peptides, RPYLKVFNPR (883-892) (P1) and RRPYLKVFNPRR (882-893) (P2), corresponding to the indicated fragments of the ceruloplasmin sequence, to affect neutrophil activation...
January 10, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177759/use-of-substitute-nonidet-p-40-nonionic-detergents-in-intracellular-tubulin-polymerization-assays-for-screening-of-microtubule-targeting-agents
#9
Saptarshi Sinha, Jessica J Field, John H Miller
Shell Chemical Company Nonidet P-40 has been used for decades in many biochemical assays as a nonionic, nondenaturing detergent; however, Shell no longer produces this product. Four commercially available substitutes were investigated and their activities titrated in an intracellular tubulin polymerization assay. Although claimed by the supply companies to be identical to the Shell Nonidet P-40, all four substitutes were about 10-fold more potent and needed to be diluted accordingly. As microtubule targeting drugs are a major class of anticancer agent, and many researchers use the intracellular tubulin polymerization assay, this information is important to help troubleshoot assay development with the new substitutes...
January 10, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177761/temporo-spacial-micoranatomical-distribution-of-the-murine-sodium-dependent-ascorbic-acid-transporters-slc23a1-and-slc23a2-in-the-kidney-throughout-development
#10
Peter Karl Eck, Christopher Corpe, Mark Alan Levine
The two membrane transporters Slc23a1 and Slc23a2 mediate ascorbic acid uptake into cells. We recently determined the key role of Slc23a1 in renal re-absorption of ascorbic acid in a knockout mouse model. However, the renal spatial and temporal expression patterns of murine Slc23a1 and Slc23a2 are not defined. This study utilizes database evidence combined with experimental confirmation via in situ hybridization to define spatial and temporal expression of Slc23a1 in the murine kidney. Slc23a1 is expressed in the early proximal tubule, but not in its precursors during embryonic development, and exclusive proximal tubular expression persists throughout the animal's lifetime...
December 20, 2016: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177762/chronic-intermittent-hypoxia-disturbs-insulin-secretion-and-causes-pancreatic-injury-via-mapk-signaling-pathway
#11
Yeying Wang, Bing Hai, Xiaoqun Niu, Li Ai, Yu Cao, Ran Li, Yongxia Li
Obstructive sleep apnea (OSA) is a breath disorder during sleep with a most prominent character of chronic intermittent hypoxia (CIH), inducing the generation of reactive oxygen species (ROS) to damage multiple tissues and cause metabolism disorders. In this study, we established rat varying OSA model by graded concentration of CIH (12.5% O2, 10% O2, 7.5%O2 and 5% O2) for 12 weeks, and found that CIH stimulated insulin secretion, reduced the insulin/proinsulin ratio in the pancreatic tissue, and caused pancreatic tissue lesions and cells apoptosis in a dose-dependent manner...
November 28, 2016: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28186858/lactoferrin-researchers-descend-on-nagoya-castle
#12
David B Alexander, Hans J Vogel, Hiroyuki Tsuda
No abstract text is available yet for this article.
February 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177763/lactoferrin-potentially-facilitates-glucose-regulation-and-enhances-the-incretin-effect
#13
Yuta Maekawa, Akihiko Sugiyama, Takashi Takeuchi
Lactoferrin (Lf) is known for its physiologically pleiotropic properties. In this study, we investigated whether Lf affects glycemic regulation, including glucose absorption from the small intestine. Bovine Lf (bLf, 100 mg/kg body mass) was administered to rats by intraperitoneal injection before intravenous (intravenous glucose tolerance test, IVGTT) or oral glucose administration (oral glucose tolerance test, OGTT). With IVGTT, bLf pretreatment had no significant effect on plasma levels of glucose or insulin...
February 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28169560/bovine-lactoferrin-and-lactoferricin-exert-antitumor-activities-on-human-colorectal-cancer-cells-ht-29-by-activating-various-signaling-pathways
#14
Rulan Jiang, Bo Lönnerdal
Lactoferrin (Lf) is an iron-binding glycoprotein that is present at high concentrations in milk. Bovine lactoferricin (LfcinB) is a peptide fragment generated by pepsin proteolysis of bovine lactoferrin (bLf). LfcinB consists of amino acid residues 17-41 proximal to the N-terminus of bLf and a disulfide bond between residues 19 and 36, forming a loop. Both bLf and LfcinB have been demonstrated to have antitumor activities. Colorectal cancer is the second most common cause of cancer death in developed countries...
February 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28169552/lactoferrin-and-the-lactoferrin-sophorolipids-assembly-can-be-internalized-by-dermal-fibroblasts-and-regulate-gene-expression
#15
Rulan Jiang, Yasushi A Suzuki, Xiaogu Du, Bo Lönnerdal
Lactoferrin (Lf) is an iron-binding multifunctional protein, mainly present in external secretions. Lf is known to penetrate skin and may thus exert its multiple functions in skin. Sophorolipids (SLs) are glycolipid biosurfactants, which have been shown to enhance absorption of commercial bovine Lf (CbLf) in model skin via forming an assembly with CbLf. In this study, uptake and post-internalization localization of bovine Lf (bLf), CbLf, and human Lf (hLf) with or without forming assemblies with SLs in human dermal fibroblasts (HDFn) were determined using (125)I-labeled Lfs and confocal microscopy, respectively...
February 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28169551/advances-in-lactoferrin-research-concerning-bovine-mastitis
#16
Kei-Ichi Shimazaki, Kazuhiro Kawai
Lactoferrin is a multifunctional, iron-binding glycoprotein found in milk and other exocrine secretions. Lactoferrin in milk plays vital roles in the healthy development of newborn mammals, and is also an innate resistance factor involved in the prevention of mammary gland infection by microorganisms. Inflammation of the udder because of bacterial infection is referred to as mastitis. There have been many investigations into the relationships between lactoferrin and mastitis, which fall into several categories...
February 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28165297/protein-surfactant-interactions-between-bovine-lactoferrin-and-sophorolipids-under-neutral-and-acidic-conditions
#17
Kentaro Matsumiya, Yasushi A Suzuki, Yoshihiko Hirata, Yuko Nambu, Yasuki Matsumura
To understand the protein-surfactant interactions between naturally derived sophorolipids (SLs) and bovine lactoferrin (bLf), we carried out spectroscopic, microscopic, and biochemical experiments under weakly acidic and neutral pH conditions. Particle size analysis, microscopy, and enzymatic digestion indicated that bLf and SLs interact with each other to form sheet-like and small aggregated structures reflecting the original self-organization of SLs at pH 5.0 and 7.0, respectively. Circular dichroism (CD) showed that SLs did not significantly affect the secondary structure of bLf...
February 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28165294/bovine-lactoferrin-and-crohn-s-disease-a-case-study
#18
David B Alexander, Masaaki Iigo, Mohamed Abdelgied, Keiji Ozeki, Satoshi Tanida, Takashi Joh, Satoru Takahashi, Hiroyuki Tsuda
A 22-year-old male suffering from abdominal pain, repeated diarrhea, and weight loss visited the Digestive Disease Department of Nagoya City University Hospital on 19 December 2011. He was hospitalized and diagnosed with Crohn's colitis. His Crohn's Disease Activity Index (CDAI) was 415. Treatment by granulocyte apheresis, mesalazine, and adalimumab was started. His CDAI was 314 on 30 December and 215 on 5 January. A colonoscopic examination on 19 January showed almost complete remission in the transverse colon and marked remission in the rectum...
February 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28165293/anticancer-activities-of-bovine-and-human-lactoferricin-derived-peptides
#19
Mauricio Arias, Ashley L Hilchie, Evan F Haney, Jan G M Bolscher, M Eric Hyndman, Robert E W Hancock, Hans J Vogel
Lactoferrin (LF) is a mammalian host defense glycoprotein with diverse biological activities. Peptides derived from the cationic region of LF possess cytotoxic activity against cancer cells in vitro and in vivo. Bovine lactoferricin (LFcinB), a peptide derived from bovine LF (bLF), exhibits broad-spectrum anticancer activity, while a similar peptide derived from human LF (hLF) is not as active. In this work, several peptides derived from the N-terminal regions of bLF and hLF were studied for their anticancer activities against leukemia and breast-cancer cells, as well as normal peripheral blood mononuclear cells...
February 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28165292/promoting-effect-of-lactoferrin-on-barrier-function-and-epithelial-differentiation-of-human-keratinocytes
#20
Ryo Uchida, Reiji Aoki, Ayako Aoki-Yoshida, Atsushi Tajima, Yoshiharu Takayama
The purpose of this study was to elucidate the effects of bovine lactoferrin on keratinocyte differentiation and barrier function. Addition of bovine lactoferrin to differentiating HaCaT human keratinocytes led to increased transepithelial electrical resistance (TER), a marker of epithelial barrier function. This elevation was followed by upregulation of two differentiation markers, involucrin and filaggrin. The expression level of sterol regulatory element-binding protein-1 was also enhanced by bovine lactoferrin...
February 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
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