journal
MENU ▼
Read by QxMD icon Read
search

Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire

journal
https://www.readbyqxmd.com/read/28544853/microrna-34a-protect-myocardial-cells-against-ischemia-reperfusion-injury-through-inhibiting-autophagy-via-regulating-tnf%C3%AE-expression
#1
Haifeng Shao, Lili Yang, Li Wang, Bozan Tang, Jian Wang, Qiang Li
BACKGROUND: Ischemia/reperfusion (I/R) accompanying with the blood supply restored after myocardial infarction. Myocardial I/R injury relieves when autophagy decreased. MicroRNA-34a(miR-34a) regulate autophagy in renal I/R injury model. But whether it can affect cardiac I/R injury remains studying. This study investigated how miR-34a in protecting myocardial cells against I/R injury through inhibit autophagy via regulating tumor necrosis factor α (TNFα) . METHODS: Constructed the I/R model in vivo with Langendorff; treated Neonatal Rat cardiomyocyte to make H/R injury model in vitro with hypoxia/reoxygenation (H/R) method...
May 25, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28538104/wnt5a-regulates-the-expression-of-ror2-tyrosine-kinase-receptor-in-ovarian-cancer-cells
#2
Ghamartaj Hossein, Somayeh Arabzadeh, Zahra Salehi-Doulabi, Zeynab Dehghani-Ghobadi, Yasaman Heidarian, Maryam Talebi-Juybari
Wnt5A and Receptor tyrosine kinase-like orphan receptor 2 (ROR2) proteins both regulate developmental processes, cell movement, and cell polarity. The purpose of the present study was to evaluate a possible regulatory role of Wnt5A on ROR2 expression in human ovarian cancer cell lines. Moreover, the expression of Wnt5A and ROR2 mRNA and protein levels were assessed in human epithelial serous ovarian cancer (HSOC) specimens. ROR2 was strongly decreased in cells treated with siRNA against Wnt5A compared to scramble-treated or lipofectamine-treated cells (P<0...
May 24, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28525726/characterization-of-an-ionic-liquid-tolerant-%C3%AE-xylosidase-from-a-marine-derived-fungal-endophyte
#3
Anindita Sengupta, Angela Zabala, Si Yu Tan, Arthur Broadstock, T S Suryanarayanan, Venkat Gopalan
Ionic liquids (ILs) are used in lignocellulosic biomass (LCB) pretreatment due to their ability to disrupt the extensive hydrogen-bonding network in cellulose and hemicellulose, and thereby decrease LCB recalcitrance to subsequent enzymatic degradation. However, this approach necessitates development of a cellulases and hemicellulases that can tolerate ~20% (w/v) IL, an amount that either co-precipitates with the sugar polymers after the initial pretreatment or is typically used in single-pot biomass deconstructions...
May 19, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28525724/interaction-between-mir-572-and-ppp2r2c-and-their-effects-on-proliferation-migration-and-invasion-of-nasopharyngeal-carcinoma-npc-cells
#4
Lei Yan, Kerui Cai, Jun Liang, Haifeng Liu, Yang Liu, Jinqiu Gui
We investigated the regulation of miR-572 on PPP2R2C and studied their effects on proliferation, migration as well as invasion of nasopharyngeal carcinoma (NPC) cells. NPC tissues and normal tissues were collected, and the expressions of miR-572 and PPP2R2C were detected by RT-PCR. And Western blot was applied to detect the expression of PPP2R2C protein. The target relationship between miR-572 and PPP2R2C was confirmed by dual luciferase reporter gene assay. MTT assay and flow cytometry was applied to investigate the viability and apoptosis of NPC cells...
May 19, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28521112/prevalence-of-externalizing-disorders-and-autism-spectrum-disorder-among-children-with-fetal-alcohol-spectrum-disorder-systematic-review-and-meta-analysis
#5
Shannon Lange, Jürgen Rehm, Evdokia Anagnostou, Svetlana Popova
Due to their central nervous system impairments, children with Fetal Alcohol Spectrum Disorder (FASD) commonly exhibit externalizing behaviours such as hyperactivity, impulsivity, and/or delinquency. The purpose of the current study was to estimate the prevalence of neurodevelopmental disorders with prominent externalizing behaviours, namely Attention-Deficit Hyperactivity Disorder (ADHD), Conduct Disorder (CD), Oppositional Defiant Disorder (ODD), as well as Autism Spectrum Disorder (ASD) among children with FASD...
May 18, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28521108/mir-301b-promotes-the-proliferation-mobility-and-epithelial-to-mesenchymal-transition-of-bladder-cancer-cells-by-targeting-egr1
#6
Lei Yan, Yan Wang, Jun Liang, Zhixin Liu, Xiaodong Sun, Kerui Cai
We aimed to investigate the role of miR-301b in the modulation of the proliferation, migration and invasion in bladder cancer (BLCA) cells. The expression of miR-301b and EGR1 (early growth response gene 1) mRNA were analyzed by qRT-PCR (quantitative real-time polymerase chain reaction). Dual-luciferase reporter gene system was used to identify the target relationship between miR-301b and EGR1. Cell proliferation, cell cycle and apoptosis were analyzed by MTT assay, colony-forming assay and flow cytometry, respectively...
May 18, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28482162/mir-129-5p-suppresses-gastric-cancer-cell-invasion-and-proliferation-by-inhibiting-col1a1
#7
Quan Wang, Jinhai Yu
In this study, we aimed to explore the role of miR-129-5p, a newly identified miR-129 member, in GC cells as well as the potential mechanism. The results of RT-qPCR and Western Blot showed that miR-129 was downregulated in gastric cancer (GC) cells compared with normal ones. Using MTT, colony formation, wound healing assay and Transwell assay, we evaluated the proliferation, migration and invasion abilities of transfected cells and confirmed miR-129-5p as a tumor suppressor in GC. Measured by RT-qPCR and Western Blot, COL1A1 turned out to be upregulated in GC tissues and cells...
May 8, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28467858/anti-angiogenic-effect-of-chebulagic-acid-involves-inhibition-of-vegfr2-and-gsk3%C3%AE-dependent-signaling-pathways
#8
A P Athira, C S Abhinand, K Saja, A Helen, P Reddanna, Perumana R Sudhakaran
Inhibition of angiogenesis is a useful strategy to prevent cancer growth, which targets new vessels that grow to nourish actively proliferating tumor cells. Endothelial cells can use a number of different pathways to cause angiogenesis and each step in these pathways can be targeted. The use of multi targeted drugs is gaining much importance in this scenario. Our previous results have shown the anti angiogenic effect of Chebulagic acid - a benzopyran tannin present in the fruits of Terminalia chebula. The present study was designed to examine the molecular mechanism of the anti angiogenic effect of chebulagic acid...
May 3, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28460187/tlr2-affects-cd86-expression-and-inflammatory-response-in-burn-injury-mice-through-regulation-of-p38
#9
Li Li, Gang Xu, Chenwang Duan
The aim of this study was to assess the effects of TLR2/p38/CD86 signaling pathways on inflammatory response in a mouse model of burn injury. Wild-type (TLR2+/+) and mutant-type (TLR2-/-) mice were obtained, and a mouse burn injury model was constructed. Tissue samples were detected using HE staining and Transferase Mediated Nick End Labeling (TUNEL) method. Macrophages were treated with TLR2 agonist and p38 inhibitor. The expression levels of TLR2, p38, CD86, IL-1β and TNF-α were identified by RT-qPCR, Western blot and ELISA...
May 1, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28423281/moringa-olifera-leaves-aqueous-extract-ameliorates-hepatotoxicity-in-alloxan-induced-diabetic-rats
#10
Mabrouk Attia Abd Eldaim, Ahmed Shaban, Samy Abd Elaziz
This study was carried out to evaluate the possible mechanisms through which the Moringa oleifera leaves aqueous extract reveals its hepatoprotective effects in alloxan induced diabetic rats. Eighty albino rats were assigned into four groups. The control group was given sterile saline. The second group was injected with alloxan (150 mg/kg BW) i.p. The third group was given Moringa oleifera leaves aqueous extract (250 mg/kg BW) orally daily. The fourth group was injected with alloxan as the second group and administrated Moringa oleifera leaves aqueous extract as in the third group...
April 19, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28380310/gng11-g-protein-%C3%AE-subunit-11-suppresses-cell-growth-with-induction-of-reactive-oxygen-species-and-abnormal-nuclear-morphology-in-human-susm-1-cells
#11
Yuki Takauji, Ikuru Kudo, Atsuki En, Ryo Matsuo, Mohammad Hossain, Kazuhiko Nakabayashi, Kensuke Miki, Michihiko Fujii, Dai Ayusawa
Enforced expression of GNG11, G-protein γ subunit 11, induces cellular senescence in normal human diploid fibroblasts. We here examined the effect of the expression of GNG11 on the growth of immortalized human cell lines, and found that it suppressed the growth of SUSM-1 cells, but not of HeLa cells. We then compared these two cell lines to understand the molecular basis for the action of GNG11. We found that expression of GNG11 induced the generation of reactive oxygen species (ROS) and abnormal nuclear morphology in SUSM-1 cells but not in HeLa cells...
April 5, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28376312/vitellogenin-receptor-selectively-endocytoses-female-specific-and-highly-expressed-hemolymph-proteins-in-the-silkworm-bombyx-mori
#12
Chaoshan Han, Enxiang Chen, Guanwang Shen, Peng Zhixin, Yinying Xu, Haiyan Zhang, Hongling Liu, Yandi Zhang, Jinxin Wu, Ying Lin, Qingyou Xia
Vitellogenin receptor (VgR), a member of the low-density lipoprotein receptor (LDLR) family, functions to transport vitellogenin into the ovaries to protome ovarian growth and embryonic development. In insects, the only widely accepted ligand of VgR is Vg. Recently, Bombyx mori VgR (BmVgR) has been shown to interact with Bombyx mori storage protein (BmSP1) in vitro. Therefore, in this study, we evaluated whether BmVgR could transport BmSP1 into certain cells. Although BmVgR could combine with Bombyx mori Vg (BmVg) and BmSP1, BmVgR did not affect the amount of BmSP1 taken up by Sf9 cells...
April 4, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28376311/contribution-of-ppar-gamma-in-modulation-of-acrolein-induced-inflammatory-signaling-in-gp91phox-knock-out-mice
#13
Zivar Yousefipour, Kasturi Ranganna, Mathew Joseph, Katarzyna Marek, Neha Chung, Alicia Nesbary, Mohammad A Newaz
Oxidative stress and inflammation are major contributors in acrolein toxicity. Peroxisome Proliferator Activated Receptor gamma (PPARγ) has antioxidant/anti-inflammatory effects. We investigated contribution of PPARγ ligand, GW1929, in attenuation of oxidative stress in acrolein-induced insult. Male gp91phox knockout (KO) mice were treated with acrolein (0.5 mg/kg; i.p.7 days) with/without GW1929 (GW, 0.5 mg/kg/day; orally-10 days). Liver was processed for further analysis. Acrolein significantly increased 8-Isoprostane and reduced PPARγ activity (P<0...
April 4, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28314112/can-mesenchymal-stem-cells-pretreated-with-platelet-rich-plasma-modulate-tissue-remodeling-in-a-rat-burn
#14
Hanan Hosni, Laila A Rashed, Soheir Mahfouz, Rania Elsayed Hussein, Marwa Alkaffas, Shaimaa Mostafa, Azza Abusree
AIM: To study the effect of PRP on BM-MSCs proliferation and to investigate their role on healing of experimental burn injury and possible mechanism of action. METHOD: Our work divided into in vitro and in vivo studies. In vitro study included,: MSCs and MSCs treated with PRP. TGF-β level and cell proliferation were assesed. In vivo study, 72 rats divided equally into 6 groups: control, burn, burn with MSCs, burn with PRP, burn with both MSCs and PRP and burn with MSCs pretreated with PRP...
March 17, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28314111/indication-for-differential-sorting-of-the-rat-v-snare-splice-isoforms-vamp-1a-and-1b
#15
Fiona R Rodepeter, Susanne Wiegand, Hans-Georg Lüers, Gabriel A Bonaterra, Anson W Lowe, Michael Bette, Ralf Jacob, Robert Mandic
SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins are essential constituents of the intracellular trafficking machinery. The variable C-terminus in the two rat VAMP-1 splice isoforms VAMP-1a and -1b potentially acts as a sorting signal since similar changes at the C-terminal end of a human VAMP-1 splice isoform resulted in its sorting to mitochondria. To evaluate differences in the subcellular localization of these two v-SNARE proteins, GFP and RFP tagged VAMP-1a and -1b proteins were expressed in HeLa, COS-7 and MDCK cells and evaluated by conventional confocal as well as total internal reflection fluorescence (TIRF) microscopy...
March 17, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28314105/u24-from-roseolovirus-interactions-with-ww-domains-canonical-vs-non-canonical
#16
Yurou Sang, Rui Zhang, A Louise Creagh, Charles A Haynes, Suzana Katarina Straus
U24 is a C-terminal membrane-anchored protein found in both Human Herpes Virus type 6 and 7 (HHV-6 and HHV-7), with an N-terminal segment that is rich in prolines (PPxY motif in both HHV-6A and 7; PxxP motif in HHV-6A). Previous work has shown that U24 interacts strongly with Nedd4 WW domains, in particular hNedd4L-WW3*. It was also shown that this interaction depends strongly on the nature of the amino acids that are upstream from the PY motif in U24. In this contribution, data was obtained from pull-downs, isothermal titration calorimetry, and NMR to further determine what modulates U24:WW domain interactions...
March 17, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28278386/structural-evaluations-of-tau-protein-conformation-methodologies-and-approaches
#17
Nicole Lauren Zabik, Matthew Martin Imhof, Sanela Martic
Protein-misfolding diseases are based on a common principle of aggregation initiated by intra- and intermolecular contacts. The structural and conformational changes, induced by biochemical transformations, such as post-translational modifications (PTMs), often lead to protein unfolding and misfolding. Thus, these order-to-disorder or disorder-to-order transitions may regulate cellular function. Tau, a neuronal protein, regulates microtubule (MT) structure and overall cellular integrity. However, misfolded tau modified by PTMs results in MT destabilization, toxic tau aggregate formation, and ultimately cell death, leading to neurodegeneration...
March 9, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28273427/genetic-variants-of-inf-%C3%AE-and-its-mrna-expression-and-inflammatory-parameters-in-vitiligo-pathogenesis
#18
Rehab Karam, Haidy Essam, Mohammed Khater
Although genetics plays an essential role in the pathogenesis of vitiligo, however, vitiligo pathogenesis is still unclear. Our aim was to investigate the role of IFN-γ expression and polymorphism in vitiligo susceptibility, and whether ICAM-1, TNF-α, and TNF-β play a role in vitiligo pathogenesis as important inflammatory parameters. Eighty-five vitiligo patients and ninety controls were investigated for IFN-γ gene expression by quantitative RT-PCR, genotyped for IFN-γ +874 T/A (rs2430561) and IFN-γ +2109A/G (rs1861494) gene polymorphisms by PCR-SSP and PCR-RFLP respectively...
March 8, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28257582/osthole-prevents-cerebral-ischemia-reperfusion-injury-via-notch-signaling-pathway
#19
Junhong Guan, Xiangtai Wei, Shengtao Qu, Tao Lv, Qiang Fu, Ye Yuan
Stroke is a common cerebrovascular disease in aged population with the second leading mortality and the leading cause of permanent disability, and the ischemic stroke is the primary form. Osthole is a coumarin derivative extracted from the fruits of Cnidium monnieri (Linn.) Cuss. In this study, we established the rat middle cerebral artery occlusion/reperfusion (MCAO/R) model in vivo, and found that MCAO/R caused cerebral infarction, hippocampus neuronal injury and apoptosis, and also activated the Notch 1 signaling pathway...
March 3, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28257581/a-modified-method-for-isolating-mouse-islets-of-an-adequate-quality-quantity-and-purity
#20
Jiejie Xu, Baogang Peng, Caiyun Zhang, Jiwei Xu, Yi Ma, Xinjun Lu
Mouse islets are widely used in diabetes research. Thus an adequate quality, quantity and purity of islets are needed for high-quality investigations. We performed a combination of filtration and density gradient separation and optimized many steps in the islet isolation procedure, including perfusion, digestion and purification. Our results show that an increased quality, quantity and purity of isolated islets can be achieved using these modifications. Moreover, this method can guarantee maximal recovery and purity of the isolated islets and is easy to perform with practice...
March 3, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
journal
journal
29271
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"