Read by QxMD icon Read

Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire

Anne Könnel, Wassilina Bugaeva, Irene Luise Gügel, Katrin Philippar
By binding to and inserting into the lipid bilayer, amphiphilic α-helices of proteins are involved in curvature of biological membranes in all organisms. In particular, they are involved in establishing the complex membrane architecture of intracellular organelles like endoplasmatic reticulum, Golgi apparatus, mitochondria and chloroplasts. Thus, amphiphilic α-helices are essential for maintenance of cellular metabolism and fitness of organisms. Here we focus on the structure and function of membrane-intrinsic proteins, which are involved in membrane curvature by amphiphilic α-helices, in mitochondria and chloroplasts of the eukaryotic model organisms yeast and <i>Arabidopsis thaliana</i>...
September 12, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Rawad Lashhab, Akm Shahid Ullah, Emmanuelle C E Cordat
In the kidney, the collecting duct (CD) is composed of at least four cell types: principal, type-A intercalated cells (IC), type-B IC and non-A, non-B IC. Although this heterogeneous composition has been recognized since the end of the 19th century, the physiological role of the various cell types in the CD continues to be deciphered as of today. Principal and IC are essential in ion / water balance and acid-base homeostasis, respectively. However, recent research has revealed a striking interplay and overlap between the specific functions of these cell types...
September 12, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Navneet Sidhu, John F Dawson
A purified F-actin derived actin trimer that interacts with end-binding proteins did not activate or bind the side-binding protein myosin under rigor conditions. Remodeling of the actin trimer by the binding of gelsolin did not rescue myosin binding, nor did the use of different means of inhibiting the polymerization of the trimer. Our results demonstrate that ADP-ribosylation on all actin subunits of an F-actin derived trimer inhibits myosin binding and that the binding of DNase-I to the pointed end subunits of a crosslinked trimer also remodels the myosin binding site...
September 7, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Dongyan Huang, Shiming Yang, Zhirao Dai, Weimin Li, Rongguan Wang
OBJECTIVE: This study aimed to explore the underlying mechanism of protocadherin 17 (PCDH17) downregulated in nasopharyngeal cancer (NPC). METHODS: NPC tumor and adjacent tissue samples were collected from NPC patients who were received therapy in Chinese PLA General Hospital. Meanwhile, a normal epithelial cell lines NP96 and 6 NPC cell lines C666-1, CEN1, CEN2, HNE1, HEN2, and HONE1 were prepared. Then, the expression level of PCDH17 and the methylation level of PCDH17 promoter in both tissues samples and cell lines were determined using PCR method...
August 30, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Mianzhi Chen, Dan Du, Wen Zheng, Mingheng Liao, Ge Liang, Lu Zhang, Meng Gong
Liver co-infection by hepatitis B virus (HBV) and hepatitis D virus (HDV) can result in a severe form of hepatocellular carcinoma with poor prognosis. Co-infection with HDV and HBV causes more deleterious effects than infection with HBV alone. Clinical research has shown that glutathione S-transferase P1 (GSTP1), a tumor suppressor gene, is typically down-regulated in liver samples from hepatitis-infected patients. In the present study, our data indicated that small HDV antigen (s-HDAg) could specifically bind to GSTP1 mRNA and significantly down-regulate GSTP1 protein expression...
August 28, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Charline Mary, Christian Baron
Agrobacterium tumefaciens est un phytopathogène étudié au vu de ses nombreuses applications pour des études d'interactions hôte/pathogène, la communication bactérienne et la capacité à transférer un fragment d'ADN dans la cellule hôte avec un système de protéines membranaires, le système de sécrétion de type IV (T4SS). Le mécanisme du T4SS est similaire à celui de la transmission des gènes de résistances aux antibiotiques et les connaissances obtenues pourront ensuite être appliquées à d'autres organismes utilisant ce genre de mécanisme...
August 24, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Daniel Schill, Joshua Nord, Lisa Ann Cirillo
We have previously shown that cooperative, interdependent binding by the pioneer factors FoxO1 and FoxA1/2 is required for recruitment of RNA polymerase II and H3K27 acetylation to the promoters of insulin regulated genes. However, the underlying mechanisms are unknown. In this study, we demonstrate that, in HepG2 cells, FoxO1 and FoxA2 form a complex on DNA that is disrupted by insulin treatment. Insulin mediated phosphorylation of FoxO1 and FoxA2 does not impair their cooperative binding to mononucleosome particles assembled from the IGFBP1 promoter, indicating that direct disruption of complex formation by phosphorylation is not responsible for the loss of interdependent FoxO1:FoxA1/2 binding following insulin treatment...
August 24, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Hua Li, Jibo Wang, Xiaoru Liu, Qiang Cheng
During the pathogenetic process of varied kidney diseases, renal tubules are the major sites in response to detrimental insults, including pro-inflammatory stimuli. MicroRNA-204-5p (miR-204-5p) can be detected in the renal tubular epithelial cells in the normal kidney, its expression however is downregulated in the kidney with pathological changes. This study aimed to investigate the role of miR-204-5p in interleukin 6 (IL6)-mediated inflammatory response and chemokine production in HK-2 renal tubular cells...
August 15, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Sandra Paschkowsky, Jacqueline Melissa Hsiao, Jason C Young, Lisa Marie Munter
Proteases carry out a wide variety of physiological functions. This review presents a brief history of protease research, starting with the original discovery of pepsin in 1836. Following the path of time, we revisit how proteases were originally classified based on their catalytic mechanism and how chemical and crystallographic studies unravelled the mechanism of serine proteases. Ongoing research on proteases addresses their biological roles, small molecule inhibitors for therapeutic uses, and protein engineering to modify their activities...
August 13, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
R Todd Alexander, Howard S Young
This is a synopsis of the 61st Annual Meeting of the CSMB, Membrane Proteins in Health and Disease.
August 13, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Manal S Fawzy, Alia Ellawindy, Mohammad Hosny Hussein, Moataz S Khashana, Marwa K Darwish, Mohamed Mohamed Abdel-Daim, Eman Ali Toraih
Glioblastoma (GB) represents the most common malignant brain tumor, which, despite extensive research, remains of poor prognosis. The focus of recent studies of GB pathogenesis has shifted to the study of the role of noncoding RNAs (ncRNAs). In this study, we examined the expression levels of the microRNA miR-326 and the long ncRNA H19 (on which a miR-326 putative binding site was found by in silico analysis) in brain tumor tissue from GB patients as compared to cancer-free brain tissue. Relative expression levels of miR-326 were not found to be significantly altered in GB patients...
August 10, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Qiuxian Xie, Shanna Lin, Manjia Zheng, Qiutao Cai, Ya Tu
Accumulating evidence has demonstrated that the long non-coding RNAs (lncRNAs) participate in the initiation and progression of cancers. In this study, we found that the lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) was significantly increased in both cervical cancer tissues and cell lines. Overexpression of NEAT1 promoted the proliferation and migration of cervical cancer cells. Molecular study uncovered that NEAT1 functioned as competitive endogenous RNA (ceRNA) to bind miR-9-5p and suppress the expression of miR-9-5p...
August 10, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Muzaddid Sarker, Matt Speckert, Jan K Rainey
Apelin peptides are cognate ligands for the apelin receptor, a G-protein coupled receptor (GPCR). The apelinergic system plays critical roles in wide-ranging physiological activities including function and development of the central nervous and cardiovascular systems. Apelin is found in 13-55 residue isoforms in vivo, all of which share the C-terminal portion of the preproapelin precursor. Characterization of high-resolution structures and detergent micelle interactions of apelin-17 led to a two-step membrane-catalyzed binding and GPCR activation mechanism hypothesis recapitulated in longer isoforms...
August 9, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Dongfeng Wang, Jian Li, Fengling Cai, Zhi Xu, Li Li, Huanfeng Zhu, Wei Liu, Qingyu Xu, Jian Cao, Jingfeng Sun, Jinhai Tang
Breast cancer is the most frequent malignant disease in women worldwide. It is a heterogeneous and complex genetic disease with different molecular characteristics. MAPT-AS1, a long non-coding RNA(lncRNA) existing at the anti-sense strand of MAPT (microtubule associated protein tau) promoter region, was believed to regulate MAPT which was associated with disease state in Parkinson's disease. But the role of MAPT-AS1 in breast cancer has never been reported. In our study we found that MAPT-AS1 is overexpressed in breast cancer but not in triple negative breast cancer(TNBC), and high expression of MAPT-AS1 was correlated with better patient survival...
August 3, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Heba M Eltahir
Hepatoma derived growth factor related protein-3 (HRP-3) is a HDGF growth factor family member that is expressed mainly in nervous tissues. It shares structural similarities with HDGF but differs in its expression and scope of action. It has recently attracted more attention due to its variable roles. HRP-3 was originally reported as a mitogenic factor. However, since then, additional functions for this growth factor have been uncovered over the last decade. In addition to its mitogenic activity, other physiological functions were discovered including those related to proliferation, differentiation and maintenance of neurons, presenting it as a neurotrophic and neuroprotective growth factor...
August 3, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Debajyoti Dutta, Larry Fliegel
Na+/H+ exchanger isoform one (NHE1) is a mammalian plasma membrane protein that removes intracellular protons, thereby elevating intracellular pH (pHi). NHE1 uses the energy of allowing an extracellular sodium down its gradient into cells, to remove 1 intracellular proton. The ubiquitous protein has several important physiological and pathological influences on mammalian cells as a result of its activity. The three dimensional structure of human NHE1 (hNHE1) is not known. Here, we modeled NHE1 based on the structure of MjNhaP1 of Methanocaldoccocus jannaschii in combination with biochemical surface accessibility data...
July 30, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Mohd Afiq Hazlami Habib, Chee Yuen Gan, Aishah Abdul Latiff, Mohd Nazri Ismail
The natural rubber latex extracted from the bark of Hevea brasiliensis plays various important roles in today's modern society. Post-translational modifications (PTMs) of the latex proteins are important for the stability and functionality of the proteins. In this study, latex proteins were acquired from the C-serum, lutoids and rubber particle layers of latex and without using prior enrichment steps, they were fragmented using collision-induced dissociation (CID), higher-energy collisional dissociation (HCD) and electron-transfer dissociation (ETD) activation methods...
July 30, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Lyudmila Ounpuu, Laura Truu, Igor Shevchuk, Vladimir Chekulayev, Aleksandr Klepinin, Andre Koit, Kersti Tepp, Marju Puurand, Egle Rebane-Klemm, Tuuli Käämbre
The aim of this work was to explore the key bioenergetic properties attributed to the mitochondrial respiration in widely used Caco-2 cell line and human colorectal cancer (HCC) postoperational tissue samples. Oxygraphy and Metabolic Control Analysis (MCA) were applied to estimate the function of oxidative phosphorylation in cultured Caco-2 cells and HCC tissue samples. The mitochondria of Caco-2 cells and HCC tissues displayed larger functional activity of respiratory complex (C)II compared to CI, whereas in normal colon tissue an inverse pattern in the ratio of CI to CII activity was observed...
July 30, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Bala Meenakshi Xavier, William J Jennings, Aiman A Zein, Junmei Wang, Jyh-Yeuan Lee
The ATP-binding cassette (ABC) proteins play critical roles in maintaining lipid and sterol homeostasis in higher eukaryotes. In human, several subfamily-A and -G members function as cholesterol transporters across the cellular membranes. Deficiencies of these ABC proteins can cause dyslipidemia that is associated with health conditions such as atherosclerosis, diabetes, fatty liver disease, and neurodegeneration. The physiological roles of ABC cholesterol transporters have been implicated in mediating cholesterol efflux for reverse cholesterol transport and in maintaining membrane integrity for cell survival...
July 30, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
M Mourin, A Wai, J O'Neil, C B Schubiger, C C Häse, G Hausner, P Dibrov
The Vc-NhaP2 antiporter from Vibrio cholerae exchanges H+ for K+ or Na+ but not for the smaller Li+ . The molecular basis of this unusual selectivity remains unknown. Phyre2 and Rosetta software were used to generate a structural model of the Vc-NhaP2. The obtained model suggested that a cluster of residues from different transmembrane segments (TMSs) forms a putative cation-binding pocket in the middle of the membrane: D133 and T132 from TMS V together with D162 and E157 of TMS VI. The model also suggested that L257, G258, and N259 from TMS IX together with T276, D273, Q280, and Y251 from TMS X as well as L289 and L342 from TMS XII form a transmembrane pathway for translocated ions with a built-in filter determining cation selectivity...
July 16, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"