journal
MENU ▼
Read by QxMD icon Read
search

Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire

journal
https://www.readbyqxmd.com/read/28423281/moringa-olifera-leaves-aqueous-extract-ameliorates-hepatotoxicity-in-alloxan-induced-diabetic-rats
#1
Mabrouk Attia Abd Eldaim, Ahmed Shaban, Samy Abd Elaziz
This study was carried out to evaluate the possible mechanisms through which the Moringa oleifera leaves aqueous extract reveals its hepatoprotective effects in alloxan induced diabetic rats. Eighty albino rats were assigned into four groups. The control group was given sterile saline. The second group was injected with alloxan (150 mg/kg BW) i.p. The third group was given Moringa oleifera leaves aqueous extract (250 mg/kg BW) orally daily. The fourth group was injected with alloxan as the second group and administrated Moringa oleifera leaves aqueous extract as in the third group...
April 19, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28380310/gng11-g-protein-%C3%AE-subunit-11-suppresses-cell-growth-with-induction-of-reactive-oxygen-species-and-abnormal-nuclear-morphology-in-human-susm-1-cells
#2
Yuki Takauji, Ikuru Kudo, Atsuki En, Ryo Matsuo, Mohammad Hossain, Kazuhiko Nakabayashi, Kensuke Miki, Michihiko Fujii, Dai Ayusawa
Enforced expression of GNG11, G-protein γ subunit 11, induces cellular senescence in normal human diploid fibroblasts. We here examined the effect of the expression of GNG11 on the growth of immortalized human cell lines, and found that it suppressed the growth of SUSM-1 cells, but not of HeLa cells. We then compared these two cell lines to understand the molecular basis for the action of GNG11. We found that expression of GNG11 induced the generation of reactive oxygen species (ROS) and abnormal nuclear morphology in SUSM-1 cells but not in HeLa cells...
April 5, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28376312/vitellogenin-receptor-selectively-endocytoses-female-specific-and-highly-expressed-hemolymph-proteins-in-the-silkworm-bombyx-mori
#3
Chaoshan Han, Enxiang Chen, Guanwang Shen, Peng Zhixin, Yinying Xu, Haiyan Zhang, Hongling Liu, Yandi Zhang, Jinxin Wu, Ying Lin, Qingyou Xia
Vitellogenin receptor (VgR), a member of the low-density lipoprotein receptor (LDLR) family, functions to transport vitellogenin into the ovaries to protome ovarian growth and embryonic development. In insects, the only widely accepted ligand of VgR is Vg. Recently, Bombyx mori VgR (BmVgR) has been shown to interact with Bombyx mori storage protein (BmSP1) in vitro. Therefore, in this study, we evaluated whether BmVgR could transport BmSP1 into certain cells. Although BmVgR could combine with Bombyx mori Vg (BmVg) and BmSP1, BmVgR did not affect the amount of BmSP1 taken up by Sf9 cells...
April 4, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28376311/contribution-of-ppar-gamma-in-modulation-of-acrolein-induced-inflammatory-signaling-in-gp91phox-knock-out-mice
#4
Zivar Yousefipour, Kasturi Ranganna, Mathew Joseph, Katarzyna Marek, Neha Chung, Alicia Nesbary, Mohammad A Newaz
Oxidative stress and inflammation are major contributors in acrolein toxicity. Peroxisome Proliferator Activated Receptor gamma (PPARγ) has antioxidant/anti-inflammatory effects. We investigated contribution of PPARγ ligand, GW1929, in attenuation of oxidative stress in acrolein-induced insult. Male gp91phox knockout (KO) mice were treated with acrolein (0.5 mg/kg; i.p.7 days) with/without GW1929 (GW, 0.5 mg/kg/day; orally-10 days). Liver was processed for further analysis. Acrolein significantly increased 8-Isoprostane and reduced PPARγ activity (P<0...
April 4, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28314112/can-mesenchymal-stem-cells-pretreated-with-platelet-rich-plasma-modulate-tissue-remodeling-in-a-rat-burn
#5
Hanan Hosni, Laila A Rashed, Soheir Mahfouz, Rania Elsayed Hussein, Marwa Alkaffas, Shaimaa Mostafa, Azza Abusree
AIM: To study the effect of PRP on BM-MSCs proliferation and to investigate their role on healing of experimental burn injury and possible mechanism of action. METHOD: Our work divided into in vitro and in vivo studies. In vitro study included,: MSCs and MSCs treated with PRP. TGF-β level and cell proliferation were assesed. In vivo study, 72 rats divided equally into 6 groups: control, burn, burn with MSCs, burn with PRP, burn with both MSCs and PRP and burn with MSCs pretreated with PRP...
March 17, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28314111/indication-for-differential-sorting-of-the-rat-v-snare-splice-isoforms-vamp-1a-and-1b
#6
Fiona R Rodepeter, Susanne Wiegand, Hans-Georg Lüers, Gabriel A Bonaterra, Anson W Lowe, Michael Bette, Ralf Jacob, Robert Mandic
SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins are essential constituents of the intracellular trafficking machinery. The variable C-terminus in the two rat VAMP-1 splice isoforms VAMP-1a and -1b potentially acts as a sorting signal since similar changes at the C-terminal end of a human VAMP-1 splice isoform resulted in its sorting to mitochondria. To evaluate differences in the subcellular localization of these two v-SNARE proteins, GFP and RFP tagged VAMP-1a and -1b proteins were expressed in HeLa, COS-7 and MDCK cells and evaluated by conventional confocal as well as total internal reflection fluorescence (TIRF) microscopy...
March 17, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28314105/u24-from-roseolovirus-interactions-with-ww-domains-canonical-vs-non-canonical
#7
Yurou Sang, Rui Zhang, A Louise Creagh, Charles A Haynes, Suzana Katarina Straus
U24 is a C-terminal membrane-anchored protein found in both Human Herpes Virus type 6 and 7 (HHV-6 and HHV-7), with an N-terminal segment that is rich in prolines (PPxY motif in both HHV-6A and 7; PxxP motif in HHV-6A). Previous work has shown that U24 interacts strongly with Nedd4 WW domains, in particular hNedd4L-WW3*. It was also shown that this interaction depends strongly on the nature of the amino acids that are upstream from the PY motif in U24. In this contribution, data was obtained from pull-downs, isothermal titration calorimetry, and NMR to further determine what modulates U24:WW domain interactions...
March 17, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28278386/structural-evaluations-of-tau-protein-conformation-methodologies-and-approaches
#8
Nicole Lauren Zabik, Matthew Martin Imhof, Sanela Martic
Protein-misfolding diseases are based on a common principle of aggregation initiated by intra- and intermolecular contacts. The structural and conformational changes, induced by biochemical transformations, such as post-translational modifications (PTMs), often lead to protein unfolding and misfolding. Thus, these order-to-disorder or disorder-to-order transitions may regulate cellular function. Tau, a neuronal protein, regulates microtubule (MT) structure and overall cellular integrity. However, misfolded tau modified by PTMs results in MT destabilization, toxic tau aggregate formation, and ultimately cell death, leading to neurodegeneration...
March 9, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28273427/genetic-variants-of-inf-%C3%AE-and-its-mrna-expression-and-inflammatory-parameters-in-vitiligo-pathogenesis
#9
Rehab Karam, Haidy Essam, Mohammed Khater
Although genetics plays an essential role in the pathogenesis of vitiligo, however, vitiligo pathogenesis is still unclear. Our aim was to investigate the role of IFN-γ expression and polymorphism in vitiligo susceptibility, and whether ICAM-1, TNF-α, and TNF-β play a role in vitiligo pathogenesis as important inflammatory parameters. Eighty-five vitiligo patients and ninety controls were investigated for IFN-γ gene expression by quantitative RT-PCR, genotyped for IFN-γ +874 T/A (rs2430561) and IFN-γ +2109A/G (rs1861494) gene polymorphisms by PCR-SSP and PCR-RFLP respectively...
March 8, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28257582/osthole-prevents-cerebral-ischemia-reperfusion-injury-via-notch-signaling-pathway
#10
Junhong Guan, Xiangtai Wei, Shengtao Qu, Tao Lv, Qiang Fu, Ye Yuan
Stroke is a common cerebrovascular disease in aged population with the second leading mortality and the leading cause of permanent disability, and the ischemic stroke is the primary form. Osthole is a coumarin derivative extracted from the fruits of Cnidium monnieri (Linn.) Cuss. In this study, we established the rat middle cerebral artery occlusion/reperfusion (MCAO/R) model in vivo, and found that MCAO/R caused cerebral infarction, hippocampus neuronal injury and apoptosis, and also activated the Notch 1 signaling pathway...
March 3, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28257581/a-modified-method-for-isolating-mouse-islets-of-an-adequate-quality-quantity-and-purity
#11
Jiejie Xu, Baogang Peng, Caiyun Zhang, Jiwei Xu, Yi Ma, Xinjun Lu
Mouse islets are widely used in diabetes research. Thus an adequate quality, quantity and purity of islets are needed for high-quality investigations. We performed a combination of filtration and density gradient separation and optimized many steps in the islet isolation procedure, including perfusion, digestion and purification. Our results show that an increased quality, quantity and purity of isolated islets can be achieved using these modifications. Moreover, this method can guarantee maximal recovery and purity of the isolated islets and is easy to perform with practice...
March 3, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28178421/lactoferrin-interacts-with-splunc1-to-attenuate-lipopolysaccharide-induced-inflammation-of-human-nasal-epithelial-cells-via-down-regulated-mek1-2-mapk-signaling
#12
Yung-An Tsou, Yu-Tong Tung, Tsu-Fang Wu, Gary Ro-Lin Chang, Han-Chien Chen, Chia-Der Lin, Chih-Ho Lai, Hsiao-Ling Chen, Chuan-Mu Chen
The short palate, lung, and nasal epithelium clone 1 (SPLUNC1) protein is an important innate material in the upper airway, and lactoferrin (LF) aids the innate functions in humans. In this study, an nasal epithelial model was used to investigate how LF modulates SPLUNC1 to reduce the inflammatory process mediated by lipopolysaccharide (LPS). The inflammation of human RPMI-2650 cells was induced by LPS to evaluate SPLUNC1 expression after treating the cells with bovine LF (bLF). The interaction pathway between LF and SPLUNC1 in LPS-induced inflammation was further investigated...
February 8, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177755/modulation-of-amyloid-assembly-by-glycosaminoglycans-from-mechanism-to-biological-significance
#13
Noe Quittot, Mathew Sebastiao, Steve Bourgault
Glycosaminoglycans (GAGs) are long and unbranched polysaccharides that are abundant in the extracellular matrix and basement membrane of multicellular organisms. These linear polyanionic macromolecules are involved in many physiological functions, from cell adhesion to cellular signaling. Interestingly, amyloid fibrils extracted from patients afflicted with protein misfolding diseases are virtually always associated with GAGs. Amyloid fibrils are highly organized nanostructures that have been historically associated with pathological states, such as Alzheimer's disease and systemic amyloidoses...
February 1, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177754/comparison-of-the-effects-of-nobiletin-and-letrozole-on-the-activity-and-expression-of-aromatase-in-mcf-7-breast-cancer-cell-line
#14
Seyedeh Tayebeh Rahideh, Mohammad Keramatipour, Mitra Nourbakhsh, Fariba Koohdani, Mostafa Hoseini, Saeed Talebi, Farzad Shidfar
Nobiletin (NOB) is one of the polymethoxyflavones mainly found in citrus fruits. Aromatase or cytochrome P450 (CYP19) enzyme catalyzes the last and rate-limiting step in estrogen biosynthesis. This study was carried out to investigate the effect of NOB on the activity and expression of aromatase and compare this property with letrozole (LET) as aromatase inhibitor in MCF-7 breast cancer cell line. The cell viability was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assays...
February 1, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177753/histone-h3-3-regulates-mitotic-progression-in-mouse-embryonic-fibroblasts
#15
Aysegul Ors, Christophe Papin, Bertrand Favier, Yohan Roulland, Defne Dalkara, Mehmet Ozturk, ALi Hamiche, Stefan Dimitrov, Kiran Padmanabhan
H3.3 is a histone variant, which marks transcription start sites as well as telomeres and heterochromatic sites on the genome. H3.3 presence is thought to positively correlate with transcriptional status of its target genes. Using a conditional genetic strategy against H3.3B combined with short hairpin RNAs against H3.3A, we essentially depleted all H3.3 gene expression in mouse embryonic fibroblasts. Following nearly complete loss of H3.3 in cells, our transcriptomic analyses show very little impact on global gene expression as well as on histone variant H2A...
February 1, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28388217/special-issue-highlights-of-the-2015-asilomar-chromatin-chromosomes-and-epigenetics-conference-num%C3%A3-ro-sp%C3%A3-cial-points-saillants-de-la-conf%C3%A3-rence-2015-d-asilomar-sur-la-chromatine-les-chromosomes-et-l-%C3%A3-pig%C3%A3-n%C3%A3-tique
#16
(no author information available yet)
No abstract text is available yet for this article.
April 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28388216/special-issue-discovery-and-development-of-the-crispr-cas9-system-num%C3%A3-ro-sp%C3%A3-cial-d%C3%A3-couverte-et-mise-au-point-du-syst%C3%A3-me-crispr-cas9
#17
(no author information available yet)
No abstract text is available yet for this article.
April 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28388215/regular-papers-articles-ordinaires
#18
(no author information available yet)
No abstract text is available yet for this article.
April 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28374617/abstracts-from-the-2015-asilomar-chromatin-chromosomes-and-epigenetics-conference
#19
(no author information available yet)
No abstract text is available yet for this article.
April 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28318299/lithocholic-acid-a-new-emergent-protector-of-intestinal-calcium-absorption-under-oxidant-conditions
#20
Ana M Marchionatti, Adriana Pérez, María A Rivoira, Valeria A Rodríguez, Nori G Tolosa de Talamoni
LCA and 1,25(OH)2D3 are vitamin D receptor ligands with different binding affinity. The secosteroid stimulates intestinal Ca(2+) absorption. Whether LCA alters this process remains unknown. The aim of our work was to determine the effect of LCA on intestinal Ca(2+) absorption in the absence or presence of NaDOC, bile acid that inhibits the cation transport. The data show that LCA by itself did not alter intestinal Ca(2+) absorption, but prevented the inhibitory effect of NaDOC. The concomitant administration of LCA avoided the reduction of intestinal alkaline phosphatase activity caused by NaDOC...
April 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
journal
journal
29271
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"