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Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire

Li Jiang, Jinghui Zhang, Naifeng Hu, Aichun Liu, Hailong Zhu, Lianqiao Li, Yuyang Tian, Xue Chen, Lina Quan
CK2α is highly expressed in many malignant tumor tissues, including lymphomas and leukemia. To investigate the role of CK2α in proliferation and apoptosis of malignant lymphomas and leukemia, two lymphomas cell lines and one leukemia cell line were infected with CK2α shRNA lentivirus or negative control shRNA lentivirus, and stably infected cell lines were established. Real-time PCR and western blot results showed that the mRNA and protein levels of CK2α were significantly reduced in CK2α knockdown cells...
May 17, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Guoliang Fan, Lin Wang, Jia Xu, Ping Jiang, Wei Wang, Ying Huang, Minggang Lv, Shaoting Liu
Increasing evidence indicates that peptidylprolyl cis/trans isomerase, NIMA-interacting 1 (Pin1) plays a decisive role in a variety of cancers. Nevertheless, its function in laryngeal squamous cell carcinoma (LSCC) has not been elaborated. The aim of this study is to determine the role of Pin1 in LSCC. Here, we established stably transfected Hep-2 cells with low expression of Pin1. Intriguingly, ability of cell proliferation, migration and invasion was significantly inhibited in Pin1-silenced Hep-2 cells. Similarly, knockdown of Pin1 induced apoptosis of Hep-2 cells, as evidenced by increased expression of cleaved-caspase-3, cleaved-PARP, and bax, and decreased expression of bcl2...
May 16, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Yassine El Hiani, Emmanuel E Egom, Xian-Ping Dong
The mechanistic target of rapamycin (mTOR) is an evolutionarily conserved serine/threonine kinase that senses and integrates environmental information into cellular regulation and homeostasis. Accumulating evidence has suggested a master role of mTOR signaling in many fundamental aspects of cell biology and organismal development. mTOR deregulation is implicated in a broad range of pathological conditions, including diabetes, cancer, neurodegenerative diseases, myopathies, inflammatory, infectious and autoimmune conditions...
May 16, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Shirley Qiu, Marceline Côté
Signalling through phosphoinositide lipids is essential for regulating many cellular processes, including endosomal trafficking. A number of intracellular pathogens have found ways to subvert host trafficking pathways via exploitation of endosomal phosphoinositides. This review will discuss how pathogens such as bacteria, viruses, and eukaryotic parasites depend on endosomal phosphoinositides for infection, as well as the mechanisms through which some are able to actively manipulate these signalling lipids to facilitate invasion, survival, replication, and immune evasion...
May 10, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Yannick Andéol, Jessica Bonneau, Laurence M Gagné, Kevin Jacquet, Véronique Rivest, Marc-Étienne Huot, Carl Séguin
Metal-responsive transcription factor-1 (MTF-1) is a metal-regulatory transcription factor essential for induction of the genes encoding metallothioneins (MTs) in response to transition metal ions. Activation of MTF-1 is dependent on the interaction of zinc with the zinc fingers of the protein. In addition, phosphorylation is essential for MTF-1 transactivation. We previously showed that inhibition of phosphoinositide 3-kinase (PI3K) abrogated <i></i>Mt<i></i> expression and metal-induced MTF-1 activation in human hepatocellular carcinoma (HCC) HepG2 and mouse L cells, thus showing that the PI3K signaling pathway positively regulates MTF-1 activity and <i></i>Mt<i></i> gene expression...
April 30, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Chao Du, Danyu Ying, Yahui Guo, Yuliang Cheng, Mei Han, Weiguo Zhang, He Qian
The study aims to elucidate the intervention effect of Sporidiobolus pararoseus (S. p.) extract on the lipid metabolism in Kunming mice with high-fat diet-induced obesity with Max EPA as a control. Ten mice were randomly selected from 60 mice as control group and the remaining 50 mice were fed with a high-fat diet to establish a dyslipidemia model. After 4 weeks, these 50 mice were randomly divided into 5 groups: high-fat model group, Max EPA group, and the 3 groups of different doses of S. p. extract (LD, MD and HD)...
April 25, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Mikko Taipale
Mutations in protein coding regions underlie almost all Mendelian disorders, drive tumorigenesis, and contribute to susceptibility to common diseases. Despite the great diversity of diseases that are caused by coding mutations, the cellular processes that affect - and are affected by - pathogenic variants at the molecular level are fundamentally conserved. Experimental and computational approaches have revealed that a substantial fraction of disease mutations are not simple loss-of-function alleles. Rather, these pathogenic variants disrupt protein function in more subtle ways by tuning protein folding pathways, altering subcellular trafficking, interrupting signaling cascades, and rewiring highly connected interaction networks...
April 25, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Shu-Yin Xu, Juan Jiang, Aihua Pan, Yan Cai, Xiao-Xin Yan
Age-related dementias are now a major mortality factor among most human populations in the world, with Alzheimer's disease (AD) being the leading dementia-causing neurodegenerative disease. The pathogenic mechanism underlying dementia disorders, and AD in specific, remained largely unclear. Efforts to develop drugs targeting the major disease hallmark lesions, such as amyloid and tangle pathologies, have been unsuccessful so far. The vacuolar protein sorting 10p (Vps10p) family plays a critical role in membrane signal transduction and protein sorting and trafficking between intracellular compartments...
April 24, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Vajiheh Neshati, Samaneh Mollazadeh, Bibi Sedigheh Fazly Bazzaz, Mehrdad Iranshahi, Majid Mojarrad, Hojjat Naderi-Meshkin, Mohammad Amin Kerachian
Stem cell therapy is considered as a promising treatment for cardiovascular diseases. Adipose-derived mesenchymal stem cells (ADMSCs) have the ability to undergo cardiomyogenesis. Medicinal plants are effective and safe candidates for cell differentiation. Therefore, the aim of our study was to investigate cardiogenic effects of characterized (HPLC-UV) G. urbanum extracts on ADMSCs of adipose tissue. The methanolic extracts of the root and aerial parts of G. urbanum were obtained and MTT assay was used for studying their cytotoxic effects...
April 24, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Mabrouk Attia Abd Eldaim, Fatma Mohamed Ibrahim, Sahar H Orabi, Azza Hassan Morsi, Hesham Saad ElSabbagh
In this study the possible mechanisms through which L-carnitine ameliorates the adverse effects of high fat diet (HFD) induced obesity on rats was evaluated. 56 Wister albino rats were randomly assigned into seven groups. Control rats fed basal diet. 2nd group fed basal diet and injected with L-carnitine 200 mg/kg i.p. 3rd group fed HFD. 4th group fed HFD and injected with L-Carnitine 200 mg/kg i.p for 8 weeks. 5th group fed HFD for 10 weeks. 6th group fed HFD for 10 weeks and injected with L-Carnitine 200 mg/kg i...
April 20, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Maryam S Abunnaja, Fatemah A Alherz, Amal A El Daibani, Ahsan F Bairam, Mohammed Ibrahim Rasool, Saud A Gohal, Katsuhisa Kurogi, Yoichi Sakakibara, Masahito Suiko, Ming-Cheh Liu
The cytosolic sulfotransferase (SULT) SULT2A1 is known to mediate the sulfation of dehydroepiandrosterone (DHEA) as well as some other hydroxysteroids such as pregnenolone. The present study was designed to investigate how genetic polymorphisms of the human SULT2A1 gene may affect the sulfation of DHEA and pregnenolone. Online databases were systematically searched to identify human SULT2A1 single nucleotide polymorphisms (SNPs). Of the 98 SULT2A1 non-synonymous coding SNPs identified, seven were selected for further investigation...
April 19, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Wenyan Gao, Luding Zhang, Xiaoqian Wang, Li Yu, Changhong Wang, Yang Gong
Indirubin and isatin have been used in the treatment of inflammatory diseases due to their anti-inflammatory properties. The present study aimed to evaluate the combined effect of indirubin and isatin on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC). UC was induced by administration of 3% (w/v) DSS solution and then the model mice were administered indirubin (10 mg/kg) or/and isatin (10 mg/kg) by gavage once daily for a week. The results showed that indirubin or/and isatin significantly inhibited weight loss, lowered disease activity index (DAI), ameliorated pathological changes, decreased the levels of pro-inflammatory mediators and myeloperoxidase (MPO) activity, increased anti-inflammatory cytokine and Foxp3 expression, suppressed CD4+ T cell infiltration, and inhibited oxidative stress and epithelial cell apoptosis...
April 19, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Yunsong Zhang, Jun Fang, Huiwen Ma
Myocardial infarction (MI), a type of ischemic heart disease, is generally accompanied by cardiomyocytes apoptosis. MiRNAs play the vital roles in the development and physiology of MI. Here, we established a downregulation model of miR-182-5p in H9c2 cells under hypoxia condition for investigating the potential molecular mechanism of miR-182-5p in hypoxia-induced cardiomyocytes apoptosis (HICA). RT-qPCR indicated that miR-182-5p level exhibited a time-dependent manner increase in hypoxia-induced cells. The results of MTT and LDH assays indicated that cardiomyocytes injury was markedly amplified after hypoxia treatment...
April 19, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Emma Bondy-Chorney, Alix Denoncourt, Yuka Sai, Michael Downey
Lysine acetylation is a critical post-translation modification that can impact a protein's localization, stability and function. Originally thought to only occur on histones, we now know thousands of non-histone proteins are also acetylated. In conjunction with many other proteins, lysine acetyltransferases (KATs) are incorporated into large protein complexes that carry out these modifications. In this review we focus on the contribution of two KATs, KAT2A and KAT2B, and their potential roles in the development and progression of cancer...
April 19, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Jiazhe Song, Fang Rong Ding, Song Li, Wenzhe Li, Ning Li, Kai Xue
Stem cell biology offers promise for understanding the origins of mammary gland. However, the distribution of mammary stem cell (MaSC) activities at earlier embryonic stage has not been fully identified. As the markers for sorting adult MaSC, CD24, CD29 and CD49f have been applied to analyze fetal MaSCs. Here we explored mammary anlagen MaSCs by investigating CD24/CD49f expressions. According to the comparative analysis between adult mammary gland and fetal mammary anlagen, we found that fetal mouse mammary anlagen might possess high percentage of potential MaSCs...
April 5, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Yongjie Feng, Lin Wang, Mingyong Wang
Lung cancer is the most common cause of cancer-related death. Non-small cell lung cancer (NSCLC) accounts for 80-85% of total lung cancer cases. Dachshund homolog 1, or DACH1, is a protein encoded by the DACH1 gene in humans. DACH1 inhibits lung adenocarcinoma invasion and tumor growth, but has a lower expression in NSCLC. To investigate the mechanisms of decreased DACH1 expression, its DNA methylation patterns were investigated. The results showed a higher methylation rate in NSCLC compared to in adjacent normal lung tissues...
March 28, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Irina V Gorudko, Daria V Grigorieva, Alexey V Sokolov, Ekaterina V Shamova, Valeria A Kostevich, Igor V Kudryavtsev, Elena D Syromiatnikova, Vadim B Vasilyev, Sergey N Cherenkevich, Oleg M Panasenko
Myeloperoxidase (MPO) is an oxidant-producing enzyme that can also regulate cellular functions via its nonenzymatic effects. Mature active MPO isolated from normal human neutrophils is a 145 kDa homodimer, which consists of two identical protomers, connected by a single disulfide bond. By binding to CD11b/CD18 integrin, dimeric MPO induces neutrophil activation and adhesion augmenting leukocyte accumulation at sites of inflammation. This study was performed to compare the potency of dimeric and monomeric MPO to elicit selected neutrophil responses...
March 27, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Tanes Lima, Leonardo Silveira
The main goal of this study was to develop a straightforward and rapid microplate assay for measuring propidium iodide (PI) in C2C12 cells. The PI method proves to be an efficient quantitative assay for analyzing cell viability through PI fluorescence analysis. Importantly, the protocol takes less than 30 minutes, and the results are reproducible. C2C12 cells were exposed to an increasing concentration of palmitate for a period of 24 hours to induce cell death, and the PI fluorescence increased in a concentration-dependent manner...
March 22, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Teng Lv, Kejuan Song, Lili Zhang, Weihua Li, Yulong Chen, Yuchao Diao, Qin Yao, Peishu Liu
This study was to explore the roles of miRNA-34a (miR-34a) in ovarian cancer (OC) cells and uncover possible mechanisms. The proliferation of OC cells was measured with MTT assay and soft agar colony formation assay. Targetscan analysis, real-time PCR and luciferase reporter assay were used to demonstrate the downstream target of miR-34a in OC cells. HDAC1 expression level was detected by immunoblot analysis. MiR-34a inhibited the proliferation of SKOV3 and OVCA433 cells and enhanced cisplatin sensitivity of cisplatin resistant SKOV3cp cells...
March 21, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
Chenggui Miao, Jun Chang, Guoxue Zhang, Yanxi Fang
MicroRNAs (miRNAs) are a class of noncoding single-stranded RNA molecules encoded by endogenous genes of about 22 nucleotides, which are involved in post-transcriptional gene expression regulation in animals and plants. Type 1 diabetes (T1D) is an autoimmune disease that is clinically silent until the majority of β cells are destroyed, and a large number of studies have shown that miRNAs are involved in the pathological mechanism of T1D. In this review, we searched the related research in recent years and summarized the important roles of miRNAs in T1D diagnosis and treatment...
March 19, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
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