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Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire

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https://www.readbyqxmd.com/read/28318299/lithocholic-acid-a-new-emergent-protector-of-intestinal-calcium-absorption-under-oxidant-conditions
#1
Ana M Marchionatti, Adriana Pérez, María A Rivoira, Valeria A Rodríguez, Nori G Tolosa de Talamoni
LCA and 1,25(OH)2D3 are vitamin D receptor ligands with different binding affinity. The secosteroid stimulates intestinal Ca(2+) absorption. Whether LCA alters this process remains unknown. The aim of our work was to determine the effect of LCA on intestinal Ca(2+) absorption in the absence or presence of NaDOC, bile acid that inhibits the cation transport. The data show that LCA by itself did not alter intestinal Ca(2+) absorption, but prevented the inhibitory effect of NaDOC. The concomitant administration of LCA avoided the reduction of intestinal alkaline phosphatase activity caused by NaDOC...
March 20, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28314112/can-mesenchymal-stem-cells-pretreated-with-platelet-rich-plasma-modulate-tissue-remodeling-in-a-rat-burn
#2
Hanan Hosni, Laila A Rashed, Soheir Mahfouz, Rania Elsayed Hussein, Marwa Alkaffas, Shaimaa Mostafa, Azza Abusree
AIM: To study the effect of PRP on BM-MSCs proliferation and to investigate their role on healing of experimental burn injury and possible mechanism of action. METHOD: Our work divided into in vitro and in vivo studies. In vitro study included,: MSCs and MSCs treated with PRP. TGF-β level and cell proliferation were assesed. In vivo study, 72 rats divided equally into 6 groups: control, burn, burn with MSCs, burn with PRP, burn with both MSCs and PRP and burn with MSCs pretreated with PRP...
March 17, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28314111/indication-for-differential-sorting-of-the-rat-v-snare-splice-isoforms-vamp-1a-and-1b
#3
Fiona R Rodepeter, Susanne Wiegand, Hans-Georg Lüers, Gabriel A Bonaterra, Anson W Lowe, Michael Bette, Ralf Jacob, Robert Mandic
SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins are essential constituents of the intracellular trafficking machinery. The variable C-terminus in the two rat VAMP-1 splice isoforms VAMP-1a and -1b potentially acts as a sorting signal since similar changes at the C-terminal end of a human VAMP-1 splice isoform resulted in its sorting to mitochondria. To evaluate differences in the subcellular localization of these two v-SNARE proteins, GFP and RFP tagged VAMP-1a and -1b proteins were expressed in HeLa, COS-7 and MDCK cells and evaluated by conventional confocal as well as total internal reflection fluorescence (TIRF) microscopy...
March 17, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28314105/u24-from-roseolovirus-interactions-with-ww-domains-canonical-vs-non-canonical
#4
Yurou Sang, Rui Zhang, A Louise Creagh, Charles A Haynes, Suzana Katarina Straus
U24 is a C-terminal membrane-anchored protein found in both Human Herpes Virus type 6 and 7 (HHV-6 and HHV-7), with an N-terminal segment that is rich in prolines (PPxY motif in both HHV-6A and 7; PxxP motif in HHV-6A). Previous work has shown that U24 interacts strongly with Nedd4 WW domains, in particular hNedd4L-WW3*. It was also shown that this interaction depends strongly on the nature of the amino acids that are upstream from the PY motif in U24. In this contribution, data was obtained from pull-downs, isothermal titration calorimetry, and NMR to further determine what modulates U24:WW domain interactions...
March 17, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28278386/structural-evaluations-of-tau-protein-conformation-methodologies-and-approaches
#5
Nicole Lauren Zabik, Matthew Martin Imhof, Sanela Martic
Protein-misfolding diseases are based on a common principle of aggregation initiated by intra- and intermolecular contacts. The structural and conformational changes, induced by biochemical transformations, such as post-translational modifications (PTMs), often lead to protein unfolding and misfolding. Thus, these order-to-disorder or disorder-to-order transitions may regulate cellular function. Tau, a neuronal protein, regulates microtubule (MT) structure and overall cellular integrity. However, misfolded tau modified by PTMs results in MT destabilization, toxic tau aggregate formation, and ultimately cell death, leading to neurodegeneration...
March 9, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28273427/genetic-variants-of-inf-%C3%AE-and-its-mrna-expression-and-inflammatory-parameters-in-vitiligo-pathogenesis
#6
Rehab Karam, Haidy Essam, Mohammed Khater
Although genetics plays an essential role in the pathogenesis of vitiligo, however, vitiligo pathogenesis is still unclear. Our aim was to investigate the role of IFN-γ expression and polymorphism in vitiligo susceptibility, and whether ICAM-1, TNF-α, and TNF-β play a role in vitiligo pathogenesis as important inflammatory parameters. Eighty-five vitiligo patients and ninety controls were investigated for IFN-γ gene expression by quantitative RT-PCR, genotyped for IFN-γ +874 T/A (rs2430561) and IFN-γ +2109A/G (rs1861494) gene polymorphisms by PCR-SSP and PCR-RFLP respectively...
March 8, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28257582/osthole-prevents-cerebral-ischemia-reperfusion-injury-via-notch-signaling-pathway
#7
Junhong Guan, Xiangtai Wei, Shengtao Qu, Tao Lv, Qiang Fu, Ye Yuan
Stroke is a common cerebrovascular disease in aged population with the second leading mortality and the leading cause of permanent disability, and the ischemic stroke is the primary form. Osthole is a coumarin derivative extracted from the fruits of Cnidium monnieri (Linn.) Cuss. In this study, we established the rat middle cerebral artery occlusion/reperfusion (MCAO/R) model in vivo, and found that MCAO/R caused cerebral infarction, hippocampus neuronal injury and apoptosis, and also activated the Notch 1 signaling pathway...
March 3, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28257581/a-modified-method-for-isolating-mouse-islets-of-an-adequate-quality-quantity-and-purity
#8
Jiejie Xu, Baogang Peng, Caiyun Zhang, Jiwei Xu, Yi Ma, Xinjun Lu
Mouse islets are widely used in diabetes research. Thus an adequate quality, quantity and purity of islets are needed for high-quality investigations. We performed a combination of filtration and density gradient separation and optimized many steps in the islet isolation procedure, including perfusion, digestion and purification. Our results show that an increased quality, quantity and purity of isolated islets can be achieved using these modifications. Moreover, this method can guarantee maximal recovery and purity of the isolated islets and is easy to perform with practice...
March 3, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28178421/lactoferrin-interacts-with-splunc1-to-attenuate-lipopolysaccharide-induced-inflammation-of-human-nasal-epithelial-cells-via-down-regulated-mek1-2-mapk-signaling
#9
Yung-An Tsou, Yu-Tong Tung, Tsu-Fang Wu, Gary Ro-Lin Chang, Han-Chien Chen, Chia-Der Lin, Chih-Ho Lai, Hsiao-Ling Chen, Chuan-Mu Chen
The short palate, lung, and nasal epithelium clone 1 (SPLUNC1) protein is an important innate material in the upper airway, and lactoferrin (LF) aids the innate functions in humans. In this study, an nasal epithelial model was used to investigate how LF modulates SPLUNC1 to reduce the inflammatory process mediated by lipopolysaccharide (LPS). The inflammation of human RPMI-2650 cells was induced by LPS to evaluate SPLUNC1 expression after treating the cells with bovine LF (bLF). The interaction pathway between LF and SPLUNC1 in LPS-induced inflammation was further investigated...
February 8, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177755/modulation-of-amyloid-assembly-by-glycosaminoglycans-from-mechanism-to-biological-significance
#10
Noe Quittot, Mathew Sebastiao, Steve Bourgault
Glycosaminoglycans (GAGs) are long and unbranched polysaccharides that are abundant in the extracellular matrix and basement membrane of multicellular organisms. These linear polyanionic macromolecules are involved in many physiological functions, from cell adhesion to cellular signaling. Interestingly, amyloid fibrils extracted from patients afflicted with protein misfolding diseases are virtually always associated with GAGs. Amyloid fibrils are highly organized nanostructures that have been historically associated with pathological states, such as Alzheimer's disease and systemic amyloidoses...
February 1, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177754/comparison-of-the-effects-of-nobiletin-and-letrozole-on-the-activity-and-expression-of-aromatase-in-mcf-7-breast-cancer-cell-line
#11
Seyedeh Tayebeh Rahideh, Mohammad Keramatipour, Mitra Nourbakhsh, Fariba Koohdani, Mostafa Hoseini, Saeed Talebi, Farzad Shidfar
Nobiletin (NOB) is one of the polymethoxyflavones mainly found in citrus fruits. Aromatase or cytochrome P450 (CYP19) enzyme catalyzes the last and rate-limiting step in estrogen biosynthesis. This study was carried out to investigate the effect of NOB on the activity and expression of aromatase and compare this property with letrozole (LET) as aromatase inhibitor in MCF-7 breast cancer cell line. The cell viability was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assays...
February 1, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177753/histone-h3-3-regulates-mitotic-progression-in-mouse-embryonic-fibroblasts
#12
Aysegul Ors, Christophe Papin, Bertrand Favier, Yohan Roulland, Defne Dalkara, Mehmet Ozturk, ALi Hamiche, Stefan Dimitrov, Kiran Padmanabhan
H3.3 is a histone variant, which marks transcription start sites as well as telomeres and heterochromatic sites on the genome. H3.3 presence is thought to positively correlate with transcriptional status of its target genes. Using a conditional genetic strategy against H3.3B combined with short hairpin RNAs against H3.3A, we essentially depleted all H3.3 gene expression in mouse embryonic fibroblasts. Following nearly complete loss of H3.3 in cells, our transcriptomic analyses show very little impact on global gene expression as well as on histone variant H2A...
February 1, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177756/method-comparison-for-analyzing-wound-healing-rates
#13
Prabhpreet K Dhillon, Xinyin Li, Jurgen T Sanes, Oluwafemi S Akintola, Bingyun Sun
Wound healing scratch assay is a frequently used method to characterize cell migration, which is an important biological process in the course of development, tissue repair, and immune response for example. The measurement of wound healing rate, however, varies among different studies. Here we summarized these measurements into three types: I) Direct Rate Average; II) Regression Rate Average; and III) Average Distance Regression Rate. Using Chinese Hamster Ovary (CHO) cells as a model, we compared the three types of analyses on quantifying the wound closing rate, and discovered that type I & III measurements are more resistant to outliers and type II analysis is more sensitive to outliers...
January 30, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177757/microrna-181-inhibits-proliferation-and-promotes-apoptosis-of-chondrocytes-in-osteoarthritis-by-targeting-pten
#14
Xiao-Feng Wu, Zi-Hui Zhou, Jian Zou
OBJECTIVE: To investigate the effects of microRNA-181 (miR-181) on proliferation and apoptosis of chondrocytes in osteoarthritis (OA) by targeting PTEN. METHODS: The chondrocytes in logarithmic growth phase were selected and divided into 6 groups: the normal, blank, negative control, miR-181 mimics, miR-181 inhibitors and miR-181 inhibitors + PTEN-siRNA groups. RT-qPCR was used to detect the expressions of miR-181 and PTEN mRNA. MTT assay and flow cytometry were performed to detect cell proliferation and apoptosis...
January 25, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177758/differential-effect-of-hypoxia-and-acidity-on-lung-cancer-cell-and-fibroblast-metabolism
#15
Alexandra Giatromanolaki, Maria Liousia, Stella Arelaki, Dimitra Kalamida, Stamatia Pouliliou, Axilleas Mitrakas, Avgi Tsolou, Efthimios Sivridis, Michael I Koukourakis
This study examined the metabolic response of lung cancer cells and normal lung fibroblasts to hypoxia and acidity. GLUT1 and HXKII mRNA/protein expression was up-regulated under hypoxia in the MRC5 fibroblasts and in the A549 and H1299 lung cancer cell lines, indicating intensified glucose absorption and glycolysis. Under hypoxia, the LDHA mRNA and LDH5 protein levels increased in the cancer cells but not in the fibroblasts. Acidity suppressed the above mentioned hypoxia effect. PDH-kinase-1 (PDK1 mRNA and protein) and inactive phosphorylated-PDH protein levels were induced under hypoxia in the cancer cells, whilst these were reduced in the MRC5 lung fibroblasts...
January 24, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177760/ceruloplasmin-derived-peptide-is-the-strongest-regulator-of-oxidative-stress-and-leukotriene-synthesis-in-neutrophils
#16
Ekaterina Golenkina, Alexey Livenskyi, Galina Viryasova, Yulia Romanova, Galina Sud'ina, Alexey Sokolov
Ceruloplasmin, an acute-phase protein, can affect the activity of leukocytes through its various enzymatic activities and protein-protein interactions (with lactoferrin, myeloperoxidase, eosinophil peroxidase, serprocidins, and 5-lipoxygenase (5-LOX), among others). However, the molecular mechanisms of ceruloplasmin activity are not clearly understood. In this study, we tested the ability of two synthetic peptides, RPYLKVFNPR (883-892) (P1) and RRPYLKVFNPRR (882-893) (P2), corresponding to the indicated fragments of the ceruloplasmin sequence, to affect neutrophil activation...
January 10, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177759/use-of-substitute-nonidet-p-40-nonionic-detergents-in-intracellular-tubulin-polymerization-assays-for-screening-of-microtubule-targeting-agents
#17
Saptarshi Sinha, Jessica J Field, John H Miller
Shell Chemical Company Nonidet P-40 has been used for decades in many biochemical assays as a nonionic, nondenaturing detergent; however, Shell no longer produces this product. Four commercially available substitutes were investigated and their activities titrated in an intracellular tubulin polymerization assay. Although claimed by the supply companies to be identical to the Shell Nonidet P-40, all four substitutes were about 10-fold more potent and needed to be diluted accordingly. As microtubule targeting drugs are a major class of anticancer agent, and many researchers use the intracellular tubulin polymerization assay, this information is important to help troubleshoot assay development with the new substitutes...
January 10, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28186858/lactoferrin-researchers-descend-on-nagoya-castle
#18
David B Alexander, Hans J Vogel, Hiroyuki Tsuda
No abstract text is available yet for this article.
February 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177763/lactoferrin-potentially-facilitates-glucose-regulation-and-enhances-the-incretin-effect
#19
Yuta Maekawa, Akihiko Sugiyama, Takashi Takeuchi
Lactoferrin (Lf) is known for its physiologically pleiotropic properties. In this study, we investigated whether Lf affects glycemic regulation, including glucose absorption from the small intestine. Bovine Lf (bLf, 100 mg/kg body mass) was administered to rats by intraperitoneal injection before intravenous (intravenous glucose tolerance test, IVGTT) or oral glucose administration (oral glucose tolerance test, OGTT). With IVGTT, bLf pretreatment had no significant effect on plasma levels of glucose or insulin...
February 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28169560/bovine-lactoferrin-and-lactoferricin-exert-antitumor-activities-on-human-colorectal-cancer-cells-ht-29-by-activating-various-signaling-pathways
#20
Rulan Jiang, Bo Lönnerdal
Lactoferrin (Lf) is an iron-binding glycoprotein that is present at high concentrations in milk. Bovine lactoferricin (LfcinB) is a peptide fragment generated by pepsin proteolysis of bovine lactoferrin (bLf). LfcinB consists of amino acid residues 17-41 proximal to the N-terminus of bLf and a disulfide bond between residues 19 and 36, forming a loop. Both bLf and LfcinB have been demonstrated to have antitumor activities. Colorectal cancer is the second most common cause of cancer death in developed countries...
February 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
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