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Glycoconjugate Journal

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https://www.readbyqxmd.com/read/28523604/chemo-enzymatic-synthesis-of-a-glycosylated-peptide-containing-a-complex-n-glycan-based-on-unprotected-oligosaccharides-by-using-dmt-mm-and-endo-m
#1
Yusuke Tomabechi, Toshihiko Katoh, Munetaka Kunishima, Toshiyuki Inazu, Kenji Yamamoto
For chemo-enzymatic synthesis of a glycosylated peptide, 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) was used for the synthesis of a N-acetylglucosaminyl peptide and a pseudoglycopeptide by solid-phase peptide synthesis without the requirement of protecting groups on the carbohydrate. We also performed transglycosylation of an N-glycan to the N-acetylglucosaminyl peptide using endo-β-N-acetylglucosaminidase from Mucor hiemalis (Endo-M) to synthesize a glycopeptide containing a complex N-glycan...
May 18, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28493026/structural-and-functional-characterization-of-a-novel-immunomodulatory-glycoprotein-isolated-from-ajowan-trachyspermum-ammi-l
#2
R R Shruthi, Y P Venkatesh, G Muralikrishna
Ajowan (Trachyspermum ammi L.) spice has been used in food preparations and also as a traditional medicine in Ayurveda. Although a number of pharmacological activities have been attributed to ajowan, its role in immunomodulation is not known. The main objective of the present study is to examine the macromolecular immunomodulatory components. Macrophage activation was studied by nitric oxide (NO) release, phagocytosis and secretion of pro-inflammatory cytokines as the markers. Ethanol precipitate (fractional) of ajowan aqueous extract was subjected to conventional chromatography (Q Sepharose followed by Bio-Gel P-100)...
May 10, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28455724/reevaluation-of-pholiota-squarrosa-lectin-reactive-haptoglobin-as-a-pancreatic-cancer-biomarker-using-an-improved-elisa-system
#3
Ken Kusama, Yuki Okamoto, Keiko Saito, Tsukasa Kasahara, Teizo Murata, Yasushi Ueno, Yuka Kobayashi, Yoshihiro Kamada, Eiji Miyoshi
An increase in Lewis- and core-type fucosylation of haptoglobin has been reported in patients with pancreatic cancer (PC), suggesting that fucosylated haptoglobin is a candidate PC biomarker. Previously, we developed a Pholiota squarrosa lectin antibody enzyme-linked immunosorbent assay (PhoSL-ELISA) system for the detection of core-fucosylated haptoglobin. However, with this methodology, positive results were only obtained for some patients with PC, demonstrating the need for a more sensitive detection system...
April 28, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28401373/sulfated-glycosaminoglycans-in-protein-aggregation-diseases
#4
REVIEW
Kazuchika Nishitsuji, Kenji Uchimura
Protein aggregation diseases are characterized by intracellular or extracellular deposition of misfolded and aggregated proteins. These aggregated deposits contain multiple proteinaceous and non-protein components that are thought to play critical roles in the etiology and pathogenesis of protein aggregation diseases in vivo. One of these components, the sulfated glycosaminoglycans (GAGs), includes heparan sulfate, chondroitin sulfate, and keratan sulfate. The sulfated GAGs are negatively charged heteropolysaccharides expressed in all mammalian tissues...
April 11, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28389847/a-pilot-study-of-salivary-n-glycome-in-hbv-induced-chronic-hepatitis-cirrhosis-and-hepatocellular-carcinoma
#5
Yannan Qin, Yaogang Zhong, Tianran Ma, Jiaxu Zhang, Ganglong Yang, Feng Guan, Zheng Li, Baozhen Li
Hepatitis B is a potentially life-threatening liver infection caused by the hepatitis B virus (HBV), which can lead to chronic liver disease and put people at high risk of death from cirrhosis of the liver and liver cancer. However, little is known about the correlation of salivary N-linked glycans related to HBV-infected liver diseases. Here we investigated N-linked glycome in saliva from 200 subjects (50 healthy volunteers (HV), 40 HBV-infected patients (HB), 50 cirrhosis patients (HC), and 60 hepatocellular carcinoma patients (HCC) using MALDI-TOF/TOF-MS...
April 8, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28349379/ulva-pertusa-lectin-1-delivery-through-adenovirus-vector-affects-multiple-signaling-pathways-in-cancer-cells
#6
Gongchu Li, Zhenzhen Zhao, Bingbing Wu, Qunshu Su, Liqin Wu, Xinyan Yang, Jing Chen
Ulva pertusa lectin 1 (UPL1) is a N-acetyl-D-glucosamine (GlcNAc) binding lectin in marine green alga Ulva pertusa. Exogenous UPL1 colocalized with protein arginine methyltransferase 5 (PRMT5), methylosome protein 50 (MEP50), β-actin and β-tubulin, indicating the interaction of UPL1 with the methylosome and cytoskeleton. UPL1 delivery through adenovirus vector (Ad-UPL1) dramatically induced extracellularly regulated protein kinases 1/2 (ERK1/2) phosphorylation in liver cancer cell lines BEL-7404 and Huh7...
March 27, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28299519/insights-into-the-effects-of-glycosylation-and-the-monosaccharide-binding-activity-of-the-plant-lectin-cratabl
#7
Laercio Pol-Fachin
CrataBL is a glycoprotein isolated from Crataeva tapia bark, containing two N-glycosylation sites. It has been identified to present lectin activity with some specificity for binding glucose over galactose. However, to date, no information on the effects of glycosylation or CrataBL monosaccharide-binding sites and monosaccharide specificity has been obtained. Thus, molecular docking and molecular dynamics simulations were employed to characterize the glycosylated CrataBL conformation and dynamics in aqueous solutions, as well as the molecular basis for its binding specificity...
March 15, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28293867/binding-specificity-of-r-10g-and-tra-1-60-81-and-substrate-specificity-of-keratanase-ii-studied-with-chemically-synthesized-oligosaccharides
#8
Hiromi Nakao, Yuko Nagai, Aya Kojima, Hidenao Toyoda, Nobuko Kawasaki, Toshisuke Kawasaki
Recently, we established a mouse monoclonal antibody specific to hiPS/ hES cells, R-10G, which recognizes a type of keratan sulfate. Keratan sulfates (KS) comprise a family of glycosaminoglycans consisting of the repeating unit of [Gal-GlcNAc(6S)]. However, there is a diversity in the degree of sulfation at Gal and GlcNAc residues, and also in the mode of linkage, Galβ1 - 3GlcNAc (type 1) or Galβ1 - 4GlcNAc (type 2). To gain more insight into the binding specificity of R-10G, we carried out an ELISA test on avidin-coated plates using polyethylene glycol (PEG)3-biotinylated derivatives of a series of N-acetyllactosamine tetrasaccharides (keratan sulfates (KSs))...
March 14, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28265838/glycosaminoglycans-major-biological-players
#9
EDITORIAL
Sylvie Ricard-Blum
No abstract text is available yet for this article.
March 6, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28194603/systemic-activation-of-nf-%C3%AE%C2%BAb-driven-luciferase-activity-in-transgenic-mice-fed-advanced-glycation-end-products-modified-albumin
#10
Norbert Nass, Kristina Bayreuther, Andreas Simm
Advanced glycation end products (AGEs) are stable end products of the Maillard reaction and accumulate with progressing ageing and degenerative diseases. Significant amounts of AGE-modified peptides are also consumed with processed food. AGEs bind to specific receptors, especially the receptor of AGEs (RAGE). Activation of RAGE then evokes intracellular signalling, finally resulting in the activation of the NF-κB transcription factor and therefore a proinflammatory state. We here analysed, whether NF-κB is activated in short term upon feeding an AGE-modified protein in-vivo...
April 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28205070/clinicopathological-significance-of-ganglioside-dsgb5-expression-in-renal-cell-carcinoma
#11
Jun Itoh, Akihiro Ito, Shuichi Shimada, Yoshihide Kawasaki, Narihiko Kakoi, Hideo Saito, Koji Mitsuzuka, Mika Watanabe, Makoto Satoh, Seiichi Saito, Yoichi Arai
Disialosyl globopentaosylceramide (DSGb5) is a ganglioside originally isolated from tissue extracts of renal cell carcinoma (RCC) with metastasis. Previous in vitro experiments have suggested that DSGb5 promotes metastasis by enhancing the migration of RCC cells and downregulating NK cell cytotoxicity against RCC cells. In this study, we investigated the clinicopathological significance of DSGb5 expression in RCC and outcomes of RCC patients. A total of 156 RCC patients who underwent surgical treatments at our hospital from January 2007 through December 2012 were analyzed in this study...
February 15, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/27796613/catfish-rhamnose-binding-lectin-induces-g0-1-cell-cycle-arrest-in-burkitt-s-lymphoma-cells-via-membrane-surface-gb3
#12
Shigeki Sugawara, Changhun Im, Tasuku Kawano, Takeo Tatsuta, Yasuhiro Koide, Daiki Yamamoto, Yasuhiro Ozeki, Kazuo Nitta, Masahiro Hosono
Silurus asotus egg lectin (SAL), an α-galactoside-binding protein isolated from the eggs of catfish, is a member of the rhamnose-binding lectin family that binds to Gb3 glycan (Galα1-4Galβ1-4Glc). We have previously demonstrated that SAL reduces the proliferation of Gb3-expressing Burkitt's lymphoma Raji cells and confirm here that it does not reduce their viability, indicating that unlike other lectins, it is not cytotoxic. The aim of this study was to determine the signal transduction mechanism(s) underlying this novel SAL/Gb3 binding-mediated effect profile...
February 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/27771794/an-integrated-approach-using-orthogonal-analytical-techniques-to-characterize-heparan-sulfate-structure
#13
Daniela Beccati, Miroslaw Lech, Jennifer Ozug, Nur Sibel Gunay, Jing Wang, Elaine Y Sun, Joël R Pradines, Victor Farutin, Zachary Shriver, Ganesh V Kaundinya, Ishan Capila
Heparan sulfate (HS), a glycosaminoglycan present on the surface of cells, has been postulated to have important roles in driving both normal and pathological physiologies. The chemical structure and sulfation pattern (domain structure) of HS is believed to determine its biological function, to vary across tissue types, and to be modified in the context of disease. Characterization of HS requires isolation and purification of cell surface HS as a complex mixture. This process may introduce additional chemical modification of the native residues...
February 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/27714477/targeted-site-specific-quantitation-of-n-and-o-glycopeptides-using-18-o-labeling-and-product-ion-based-mass-spectrometry
#14
Jandhyam Srikanth, Rathinasamy Agalyadevi, Ponnusamy Babu
The site-specific quantitation of N- and O-glycosylation is vital to understanding the function(s) of different glycans expressed at a given site of a protein under physiological and disease conditions. Most commonly used precursor ion intensity based quantification method is less accurate and other labeled methods are expensive and require enrichment of glycopeptides. Here, we used glycopeptide product (y and Y0) ions and (18)O-labeling of C-terminal carboxyl group as a strategy to obtain quantitative information about fold-change and relative abundance of most of the glycoforms attached to the glycopeptides...
February 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28101734/chondroitin-sulfates-and-their-binding-molecules-in-the-central-nervous-system
#15
REVIEW
L Djerbal, H Lortat-Jacob, Jcf Kwok
Chondroitin sulfate (CS) is the most abundant glycosaminoglycan (GAG) in the central nervous system (CNS) matrix. Its sulfation and epimerization patterns give rise to different forms of CS, which enables it to interact specifically and with a significant affinity with various signalling molecules in the matrix including growth factors, receptors and guidance molecules. These interactions control numerous biological and pathological processes, during development and in adulthood. In this review, we describe the specific interactions of different families of proteins involved in various physiological and cognitive mechanisms with CSs in CNS matrix...
January 18, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28091942/all-trans-retinoic-acid-supplementation-prevents-cardiac-fibrosis-and-cytokines-induced-by-methylglyoxal
#16
Umadevi Subramanian, Devipriya Nagarajan
Methylglyoxal (MG), a metabolic intermediate of glycolysis is a precursor for endogeneous production of advanced glycation end-products. The increased production of MG have negative influence over the structure and function of different biomolecules and thus plays an important role in the pathogenesis of diabetic cardiac complications. Retinoic acid (RA), an active metabolite of vitamin A, has a major role in preventing cardiac remodeling and ventricular fibrosis. Hence, the objective of the present study was to determine whether rats administered with all-trans retinoic acid (RA) could attenuate MG induced pathological effects...
January 14, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28091941/occurrence-of-complex-type-free-n-glycans-with-a-single-glcnac-residue-at-the-reducing-termini-in-the-fresh-water-plant-egeria-densa
#17
Megumi Maeda, Natsuki Ebara, Misato Tani, Christopher J Vavricka, Yoshinobu Kimura
In our previous study, we found unique free N-glycans (FNGs), which carry a single GlcNAc residue (GN1) at the reducing-end side and the Lewis-a epitope at the non-reducing-end side, in the culture broth of rice cells. Based on the FNG structural features and the substrate specificity of plant ENGase, we hypothesized that there might be a novel biosynthetic mechanism responsible for the production of these unique GN1-FNGs, in which high-mannose type (HMT)-GN1-FNGs produced in the cytosol from misfolded glycoproteins by ENGase are transported back into the endoplasmic reticulum and processed to plant complex type (PCT)-GN1-FNGs in the Golgi apparatus...
January 14, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28091940/purification-and-sequence-characterization-of-chondroitin-sulfate-and-dermatan-sulfate-from-fishes
#18
Na Lin, Xiaoli Mo, Yang Yang, Hong Zhang
Chondroitin sulfate (CS) and dermatan sulfate (DS) were extracted and purified from skins or bones of salmon (Salmo salar), snakehead (Channa argus), monkfish (Lophius litulon) and skipjack tuna (Katsuwonus pelamis). Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be characterized and identified using high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry. CS and DS chain structure varies depending on origin, but motif structure appears consistent...
January 14, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28091939/immuno-pcr-technology-for-detection-of-natural-human-antibodies-against-le-c-disaccharide
#19
Artem V Maerle, Daria V Voronina, Kira L Dobrochaeva, Oxana E Galanina, Leonid P Alekseev, Nicolai V Bovin, Sergei K Zavriev, Dmitriy Yu Ryazantsev
The development of an immuno-PCR assay for quantitation of low amounts of anti-glycan human antibodies is described. The sensitivity of the assay for determination of low-affinity anti-Le(C) IgM has been found to be 4 ng/ml (~100 pg per sample), thus being two orders of magnitude higher compared to the conventional ELISA with the same antigen.
January 14, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28078490/podocalyxin-as-a-major-pluripotent-marker-and-novel-keratan-sulfate-proteoglycan-in-human-embryonic-and-induced-pluripotent-stem-cells
#20
REVIEW
Hidenao Toyoda, Yuko Nagai, Aya Kojima, Akiko Kinoshita-Toyoda
Podocalyxin (PC) was first identified as a heavily sialylated transmembrane protein of glomerular podocytes. Recent studies suggest that PC is a remarkable glycoconjugate that acts as a universal glyco-carrier. The glycoforms of PC are responsible for multiple functions in normal tissue, human cancer cells, human embryonic stem cells (hESCs), and human induced pluripotent stem cells (hiPSCs). PC is employed as a major pluripotent marker of hESCs and hiPSCs. Among the general antibodies for human PC, TRA-1-60 and TRA-1-81 recognize the keratan sulfate (KS)-related structures...
January 11, 2017: Glycoconjugate Journal
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