journal
MENU ▼
Read by QxMD icon Read
search

Glycoconjugate Journal

journal
https://www.readbyqxmd.com/read/28624993/cancer-stem-cell-marker-glycosylation-nature-function-and-significance
#1
REVIEW
Brody W Mallard, Joe Tiralongo
Glycans are essential for the maintenance of normal biological function, with alterations in glycan expression being a hallmark of cancer. Cancer stem cells (CSCs) are a subset of cells within a tumour capable of self-renewal, cellular differentiation and resistances to conventional therapies. As is the case with stem cells, marker proteins present on the cell surface are frequently used to identify and enrich CSCs, with the expression of these markers statistical correlating with the likelihood of cancer recurrence and overall patient survival...
June 17, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28616799/dolichol-phosphate-mannose-synthase-a-glycosyltransferase-with-unity-in-molecular-diversities
#2
REVIEW
Dipak K Banerjee, Zhenbo Zhang, Krishna Baksi, Jesús E Serrano-Negrón
N-glycans provide structural and functional stability to asparagine-linked (N-linked) glycoproteins, and add flexibility. Glycan biosynthesis is elaborative, multi-compartmental and involves many glycosyltransferases. Failure to assemble N-glycans leads to phenotypic changes developing infection, cancer, congenital disorders of glycosylation (CDGs) among others. Biosynthesis of N-glycans begins at the endoplasmic reticulum (ER) with the assembly of dolichol-linked tetra-decasaccharide (Glc3Man9GlcNAc2-PP-Dol) where dolichol phosphate mannose synthase (DPMS) plays a central role...
June 14, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28597244/seasonal-and-flight-related-variation-of-galectin-expression-in-heart-liver-and-flight-muscles-of-yellow-rumped-warblers-setophaga-coronata
#3
Stefanie S Bradley, Morag F Dick, Christopher G Guglielmo, Alexander V Timoshenko
Galectins, a family of multifunctional glycan-binding proteins, are proposed as biomarkers of cellular stress responses. Avian migration is an energetically challenging physical stress, which represents a physiological model of muscular endurance exercises. This study assesses change in galectin gene expression profiles associated with seasonal variation in migratory state and endurance flight in yellow-rumped warblers (Setophaga coronata). Bioinformatics analysis and real-time qPCR were used to analyse the expression of galectins in flight muscle, heart and liver tissues of 15 warblers separated into three groups of winter unflown, and fall migratory flown/unflown birds...
June 8, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28597243/probing-the-catalytic-site-of-rabbit-muscle-glycogen-phosphorylase-using-a-series-of-specifically-modified-maltohexaose-derivatives
#4
Makoto Nakamura, Yasushi Makino, Chika Takagi, Tohru Yamagaki, Masaaki Sato
Glycogen phosphorylase (GP) is an allosteric enzyme whose catalytic site comprises six subsites (SG1, SG-1, SG-2, SG-3, SG-4, and SP) that are complementary to tandem five glucose residues and one inorganic phosphate molecule, respectively. In the catalysis of GP, the nonreducing-end glucose (Glc) of the maltooligosaccharide substrate binds to SG1 and is then phosphorolyzed to yield glucose 1-phosphate. In this study, we probed the catalytic site of rabbit muscle GP using pyridylaminated-maltohexaose (Glcα1-4Glcα1-4Glcα1-4Glcα1-4Glcα1-4GlcPA, where GlcPA = 1-deoxy-1-[(2-pyridyl)amino]-D-glucitol]; abbreviated as PA-0) and a series of specifically modified PA-0 derivatives (Glc m -AltNAc-Glc n -GlcPA, where m + n = 4 and AltNAc is 3-acetoamido-3-deoxy-D-altrose)...
June 8, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28577071/isolation-of-a-methylated-mannose-binding-protein-from-terrestrial-worm-enchytraeus-japonensis
#5
Shigeru Ogawa, Mamoru Mizuno, Mayuko Suzuki, Kohtaro Goto, Yuriko Hirose, Akio Matsuda, Takuya Saito, Suguru Oguri, Kiyoshi Furukawa
To elucidate a biological role of the methylated mannose residues found in N-glycans of terrestrial worm Enchytraeus japonensis, we first synthesized 3-O-methyl mannose- and 4-O-methyl mannose-derivatives and immobilized them to Sepharose 4B beads in order to isolate the sugar-binding protein. When whole protein extracts from the worms was applied to a series of the columns immobilized with the modified and unmodified mannose-derivatives, respectively, a protein with a molecular weight of 25,000 was isolated by 4-O-methyl mannose-immobilized column chromatography, and termed as a methylated mannose-binding protein (mMBP)...
June 2, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28573337/immunological-characterization-of-a-rigid-%C3%AE-tn-mimetic-on-murine-inkt-and-human-nk-cells
#6
Silvia Fallarini, Alvaro Brittoli, Michele Fiore, Grazia Lombardi, Olivier Renaudet, Barbara Richichi, Cristina Nativi
The ability of a rigid α-Tn mimetic (compound 1) to activate murine invariant natural killer T (iNKT) and human natural killer (NK) cells, two subsets of lymphocytes involved in cancer immunesurveillance, was investigated. For this purpose, the mimetic 1 was properly conjugated to a stearic acid containing glycerol-based phospholipid (compound 5) to be presented, in the context of the conserved non polymorphic major histocompatibility complex class I-like molecules (CD1d), to iNKT cells. On the contrary, the mimetic 1 was conjugated to a multivalent peptide-based scaffold (compound 6) to induce NK cell activation...
June 1, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28555433/structural-and-activity-variability-of-fractions-with-different-charge-density-and-chain-length-from-pharmaceutical-heparins
#7
Yanlei Yu, Makoto Hirakane, Daisuke Mori, Lei Lin, Fuming Zhang, Hong Zhang, Robert J Linhardt
Heparin is a structurally complex polysaccharide used as a clinical anticoagulant. It is comprised of a heterogeneous mixture of polysaccharide chains having a variety of sequences and lengths. The production methods and regulatory controls of pharmaceutical heparins have changed over the years. This study assesses the structural and activity uniformity of the polysaccharide chains comprising two contemporary heparin products. The heparin fractions with different sizes and charges were separated with size exclusion and ion exchange chromatography...
May 29, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28523604/chemo-enzymatic-synthesis-of-a-glycosylated-peptide-containing-a-complex-n-glycan-based-on-unprotected-oligosaccharides-by-using-dmt-mm-and-endo-m
#8
Yusuke Tomabechi, Toshihiko Katoh, Munetaka Kunishima, Toshiyuki Inazu, Kenji Yamamoto
For chemo-enzymatic synthesis of a glycosylated peptide, 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) was used for the synthesis of a N-acetylglucosaminyl peptide and a pseudoglycopeptide by solid-phase peptide synthesis without the requirement of protecting groups on the carbohydrate. We also performed transglycosylation of an N-glycan to the N-acetylglucosaminyl peptide using endo-β-N-acetylglucosaminidase from Mucor hiemalis (Endo-M) to synthesize a glycopeptide containing a complex N-glycan...
May 18, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28493026/structural-and-functional-characterization-of-a-novel-immunomodulatory-glycoprotein-isolated-from-ajowan-trachyspermum-ammi-l
#9
R R Shruthi, Y P Venkatesh, G Muralikrishna
Ajowan (Trachyspermum ammi L.) spice has been used in food preparations and also as a traditional medicine in Ayurveda. Although a number of pharmacological activities have been attributed to ajowan, its role in immunomodulation is not known. The main objective of the present study is to examine the macromolecular immunomodulatory components. Macrophage activation was studied by nitric oxide (NO) release, phagocytosis and secretion of pro-inflammatory cytokines as the markers. Ethanol precipitate (fractional) of ajowan aqueous extract was subjected to conventional chromatography (Q Sepharose followed by Bio-Gel P-100)...
May 10, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28455724/reevaluation-of-pholiota-squarrosa-lectin-reactive-haptoglobin-as-a-pancreatic-cancer-biomarker-using-an-improved-elisa-system
#10
Ken Kusama, Yuki Okamoto, Keiko Saito, Tsukasa Kasahara, Teizo Murata, Yasushi Ueno, Yuka Kobayashi, Yoshihiro Kamada, Eiji Miyoshi
An increase in Lewis- and core-type fucosylation of haptoglobin has been reported in patients with pancreatic cancer (PC), suggesting that fucosylated haptoglobin is a candidate PC biomarker. Previously, we developed a Pholiota squarrosa lectin antibody enzyme-linked immunosorbent assay (PhoSL-ELISA) system for the detection of core-fucosylated haptoglobin. However, with this methodology, positive results were only obtained for some patients with PC, demonstrating the need for a more sensitive detection system...
April 28, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28401373/sulfated-glycosaminoglycans-in-protein-aggregation-diseases
#11
REVIEW
Kazuchika Nishitsuji, Kenji Uchimura
Protein aggregation diseases are characterized by intracellular or extracellular deposition of misfolded and aggregated proteins. These aggregated deposits contain multiple proteinaceous and non-protein components that are thought to play critical roles in the etiology and pathogenesis of protein aggregation diseases in vivo. One of these components, the sulfated glycosaminoglycans (GAGs), includes heparan sulfate, chondroitin sulfate, and keratan sulfate. The sulfated GAGs are negatively charged heteropolysaccharides expressed in all mammalian tissues...
April 11, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28389847/a-pilot-study-of-salivary-n-glycome-in-hbv-induced-chronic-hepatitis-cirrhosis-and-hepatocellular-carcinoma
#12
Yannan Qin, Yaogang Zhong, Tianran Ma, Jiaxu Zhang, Ganglong Yang, Feng Guan, Zheng Li, Baozhen Li
Hepatitis B is a potentially life-threatening liver infection caused by the hepatitis B virus (HBV), which can lead to chronic liver disease and put people at high risk of death from cirrhosis of the liver and liver cancer. However, little is known about the correlation of salivary N-linked glycans related to HBV-infected liver diseases. Here we investigated N-linked glycome in saliva from 200 subjects (50 healthy volunteers (HV), 40 HBV-infected patients (HB), 50 cirrhosis patients (HC), and 60 hepatocellular carcinoma patients (HCC) using MALDI-TOF/TOF-MS...
April 8, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28577070/heparan-sulfate-proteoglycans-as-key-regulators-of-the-mesenchymal-niche-of-hematopoietic-stem-cells
#13
Dulce Papy-Garcia, Patricia Albanese
The complex microenvironment that surrounds hematopoietic stem cells (HSCs) in the bone marrow niche involves different coordinated signaling pathways. The stem cells establish permanent interactions with distinct cell types such as mesenchymal stromal cells, osteoblasts, osteoclasts or endothelial cells and with secreted regulators such as growth factors, cytokines, chemokines and their receptors. These interactions are mediated through adhesion to extracellular matrix compounds also. All these signaling pathways are important for stem cell fates such as self-renewal, proliferation or differentiation, homing and mobilization, as well as for remodeling of the niche...
June 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28265838/glycosaminoglycans-major-biological-players
#14
EDITORIAL
Sylvie Ricard-Blum
No abstract text is available yet for this article.
June 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28101734/chondroitin-sulfates-and-their-binding-molecules-in-the-central-nervous-system
#15
REVIEW
L Djerbal, H Lortat-Jacob, Jcf Kwok
Chondroitin sulfate (CS) is the most abundant glycosaminoglycan (GAG) in the central nervous system (CNS) matrix. Its sulfation and epimerization patterns give rise to different forms of CS, which enables it to interact specifically and with a significant affinity with various signalling molecules in the matrix including growth factors, receptors and guidance molecules. These interactions control numerous biological and pathological processes, during development and in adulthood. In this review, we describe the specific interactions of different families of proteins involved in various physiological and cognitive mechanisms with CSs in CNS matrix...
June 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/27928742/implementation-of-infrared-and-raman-modalities-for-glycosaminoglycan-characterization-in-complex-systems
#16
Hossam Taha Mohamed, Valérie Untereiner, Ganesh D Sockalingum, Stéphane Brézillon
Glycosaminoglycans (GAGs) are natural, linear and negatively charged heteropolysaccharides which are incident in every mammalian tissue. They consist of repeating disaccharide units, which are composed of either sulfated or non-sulfated monosaccharides. Depending on tissue types, GAGs exhibit structural heterogeneity such as the position and degree of sulfation or within their disaccharide units composition being heparin, heparan sulfate, chondroitine sulfate, dermatan sulfate, keratan sulfate, and hyaluronic acid...
June 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/27924424/rgta-%C3%A2-or-regenerating-agents-mimic-heparan-sulfate-in-regenerative-medicine-from-concept-to-curing-patients
#17
REVIEW
Denis Barritault, Marie Gilbert-Sirieix, Kim Lee Rice, Fernando Siñeriz, Dulce Papy-Garcia, Christophe Baudouin, Pascal Desgranges, Gilbert Zakine, Jean-Louis Saffar, Johan van Neck
The importance of extracellular matrix (ECM) integrity in maintaining normal tissue function is highlighted by numerous pathologies and situations of acute and chronic injury associated with dysregulation or destruction of ECM components. Heparan sulfate (HS) is a key component of the ECM, where it fulfils important functions associated with tissue homeostasis. Its degradation following tissue injury disrupts this delicate equilibrium and may impair the wound healing process. ReGeneraTing Agents (RGTA(®)s) are polysaccharides specifically designed to replace degraded HS in injured tissues...
June 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/27924423/ms-ir-a-new-ms-based-hyphenated-method-for-analysis-of-hexuronic-acid-epimers-in-glycosaminoglycans
#18
Baptiste Schindler, Gina Renois-Predelus, Nassiba Bagdadi, Sihem Melizi, Loïc Barnes, Stéphane Chambert, Abdul-Rahman Allouche, Isabelle Compagnon
We report an original MS-based hyphenated method for the elucidation of the epimerization in GAG fragments. It consists of measuring simultaneously the MS/MS spectrum and the gas phase IR spectrum to gain direct structural information. This is possible using a customized MS instrument, modified to allow injection of a tunable IR laser inside of the instrument for in situ spectroscopy of trapped ions. The proof of principle of this approach is performed in the case of a hyaluronic acid tetrasaccharide standard...
June 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/27900575/glycosaminoglycanomics-where-we-are
#19
Sylvie Ricard-Blum, Frédérique Lisacek
Glycosaminoglycans regulate numerous physiopathological processes such as development, angiogenesis, innate immunity, cancer and neurodegenerative diseases. Cell surface GAGs are involved in cell-cell and cell-matrix interactions, cell adhesion and signaling, and host-pathogen interactions. GAGs contribute to the assembly of the extracellular matrix and heparan sulfate chains are able to sequester growth factors in the ECM. Their biological activities are regulated by their interactions with proteins. The structural heterogeneity of GAGs, mostly due to chemical modifications occurring during and after their synthesis, makes the development of analytical techniques for their profiling in cells, tissues, and biological fluids, and of computational tools for mining GAG-protein interaction data very challenging...
June 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/27900574/mast-cell-glycosaminoglycans
#20
REVIEW
B Mulloy, R Lever, C P Page
Mast cells contain granules packed with a mixture of proteins that are released on degranulation. The proteoglycan serglycin carries an array of glycosaminoglycan (GAG) side chains, sometimes heparin, sometimes chondroitin or dermatan sulphate. Tight packing of granule proteins is dependent on the presence of serglycin carrying these GAGs. The GAGs of mast cells were most intensively studied in the 1970s and 1980s, and though something is known about the fine structure of chondroitin sulphate and dermatan sulphate in mast cells, little is understood about the composition of the heparin/heparan sulphate chains...
June 2017: Glycoconjugate Journal
journal
journal
29226
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"