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Glycoconjugate Journal

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https://www.readbyqxmd.com/read/29335800/enrichment-and-characterization-of-a-bacterial-mixture-capable-of-utilizing-c-mannosyl-tryptophan-as-a-carbon-source
#1
Tanim J Hossain, Shino Manabe, Yukishige Ito, Toshiya Iida, Saori Kosono, Kenji Ueda, Akira Hosomi, Daishi Inoue, Tadashi Suzuki
C-Mannosylation, a protein-modification found in various eukaryotes, involves the attachment of a single mannose molecule to selected tryptophan residues of proteins. Since C-mannosyl tryptophan (CMW) was detected in human urine, it is generally thought that CMW is not catabolized inside our body and instead is excreted via the urine. This paper reports enrichment of a bacterial consortium from soil that degrades CMW. The bacteria grew in minimal medium supplemented with CMW as the carbon source. Interestingly, even after successive clonal picks of individual colonies, several species were still present in each colony as revealed by 16S rRNA gene sequence analysis, indicating that a single species may not be responsible for this activity...
January 15, 2018: Glycoconjugate Journal
https://www.readbyqxmd.com/read/29305779/evidence-of-glucuronidation%C3%A2-of-the-glycation-product-lw-1-tentative-structure-and-implications-for-the-long-term-complications-of-diabetes
#2
David R Sell, Ina Nemet, Zhili Liang, Vincent M Monnier
LW-1 is a collagen-linked blue fluorophore whose skin levels increase with age, diabetes and end-stage renal disease (ESRD), and correlate with the long-term progression of microvascular disease and indices of subclinical cardiovascular disease in type 1 diabetes. The chemical structure of LW-1 is still elusive, but earlier NMR analyses showed it has a lysine residue in an aromatic ring coupled to a sugar molecule reminiscent of advanced glycation end-products (AGEs). We hypothesized and demonstrate here that the unknown sugar is a N-linked glucuronic acid...
January 5, 2018: Glycoconjugate Journal
https://www.readbyqxmd.com/read/29305778/advanced-glycation-end-products-as-a-source-of-artifacts-in-immunoenzymatic-methods
#3
Aleksandra Kuzan, A Chwiłkowska, K Maksymowicz, A Szydełko-Bronowicka, K Stach, C Pezowicz, A Gamian
The most abundant proteins in the arteries are those of extracellular matrix, ie. collagen and elastin. Due to their long half-lifes these proteins have an increased chance to undergo glycation. The aim of this study was to determine relationship between the content of the main extracellular matrix proteins and the advanced glycation end products (AGEs) in arteries. In this study 103 fragments of aorta were analyzed by ELISA and immunobloting for the content of collagens type I, III and IV and elastin and the content of advanced glycation end-products (AGE)...
January 5, 2018: Glycoconjugate Journal
https://www.readbyqxmd.com/read/29305777/glycosaminoglycans-from-bovine-eye-vitreous-humour-and-interaction-with-collagen-type-ii
#4
Yanfei Peng, Yanlei Yu, Lei Lin, Xinyue Liu, Xing Zhang, Peipei Wang, Pauline Hoffman, So Young Kim, Fuming Zhang, Robert J Linhardt
Glycosaminoglycans (GAGs) play an important role in stabilizing the gel state of eye vitreous humour. In this study, the composition of GAGs present in bovine eye vitreous was characterized through disaccharide analysis by liquid chromatography-mass spectrometry. The interaction of GAGs with collagen type II was assessed using surface plasmon resonance (SPR). The percentage of hyaluronic acid (HA), chondroitin sulfate (CS) and heparan sulfate (HS), of total GAG, were 96.2%, 3.5% and 0.3%, respectively. The disaccharide composition of CS consisted of 4S (49%), 0S (38%) 6S (12%), 2S6S (1...
January 5, 2018: Glycoconjugate Journal
https://www.readbyqxmd.com/read/29209879/one-pot-analysis-of-sulfated-glycosaminoglycans
#5
C B Shrikanth, J Sanjana, Nandini D Chilkunda
Routine isolation, estimation, and characterization of glycosaminoglycans (GAGs) is quite challenging. This is compounded by the fact that the analysis is technique-intensive and more often there will be a limitation on the quantity of GAGs available for various structural, functional and biological studies. In such a scenario, the sample which can be made available for estimation and elucidation of disaccharide composition and species composition as well remains a challenge. In the present study, we have determined the feasibility where isolated sulfated GAGs (sGAG) that is estimated by metachromasia is recovered for further analysis...
December 5, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/29196839/purification-and-activity-characterization-of-polysaccharides-in-the-medicinal-lichen-umbilicaria-tornata-from-taibai-mountain-china
#6
Jiao Shang, Minfeng Fang, Li Zhang, Hui Wang, Guiping Gong, Zhongfu Wang, Ajing Zhao, Huihui Yi
Water-soluble polysaccharides from Umbilicaria tornata (UTP) were purified and preliminarily characterized. The antioxidant and antitumor activities of crude UTP and two purified fractions (UTP-1 and UTP-2) were evaluated using in vitro experiments. The results showed that the molecular weights of UTP-1 and UTP-2 were 84.86 and 28.66 kDa, respectively. Both UTP-1 and UTP-2 were composed of glucose and xylose, with their molar ratios being 1.3:0.9 and 0.9:4.6, respectively. In addition, crude UTP, UTP-1 and UTP-2 showed dose-dependent DPPH and hydroxyl radical scavenging and reducing activities...
December 2, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/29124565/glycosaminoglycans-from-fish-swim-bladder-isolation-structural-characterization-and-bioactive-potential
#7
Yongxi Pan, Peipei Wang, Fuming Zhang, Yanlei Yu, Xing Zhang, Lei Lin, Robert J Linhardt
The swim bladder of fish is an internal gas-filled organ that allows fish to control their buoyancy and swimming depth. Fish maws (the dried swim bladders of fish) have been used over many centuries as traditional medicines, tonics and a luxurious gourmet food in China and Southeast Asia. Little is known about the structural information of polysaccharides comprising this important functional material of fish tissue. In the present study, the total glycosaminoglycan (GAG) from fish maw was characterized. Two GAGs were identified, chondroitin sulfate (CS, having a molecular weight of 18-40 kDa) and heparan sulfate (HS), corresponding to 95% and 5% of the total GAG, respectively...
November 10, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/29105004/special-issue-glycobiology-on-stem-cells-editorial
#8
EDITORIAL
Toshisuke Kawasaki, Robert K Yu
No abstract text is available yet for this article.
November 6, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/29063990/identification-and-biochemical-characterization-of-wbwb-a-novel-udp-gal-neu5ac-r-%C3%AE-1-4-galactosyltransferase-from-the-intestinal-pathogen-escherichia-coli-serotype-o104
#9
Diana Czuchry, Walter A Szarek, Inka Brockhausen
The intestinal pathogen Escherichia coli serotype O104:H4 (ECO104) can cause bloody diarrhea and haemolytic uremic syndrome. The ECO104 O antigen has the unique repeating unit structure [4Galα1-4Neu5,7,9Ac3α2-3Galβ1-3GalNAcβ1-], which includes the mammalian sialyl-T antigen as an internal structure. Previously, we identified WbwC from ECO104 as the β3Gal-transferase that synthesizes the T antigen, and showed that α3-sialyl-transferase WbwA transfers sialic acid to the T antigen. Here we identify the wbwB gene product as a unique α1,4-Gal-transferase WbwB that transfers Gal from UDP-Gal to the terminal sialic acid residue of Neu5Acα2-3Galβ1-3GalNAcα-diphosphate-lipid acceptor...
October 24, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/29150735/glycomics-of-human-embryonic-stem-cells-and-human-induced-pluripotent-stem-cells
#10
REVIEW
Jun-Ichi Furukawa, Kazue Okada, Yasuro Shinohara
Most cells are coated by a dense glycocalyx composed of glycoconjugates such as glycosphingolipids, glycoproteins, and proteoglycans. The overall glycomic profile is believed to be crucial for the diverse roles of glycans, which are mediated by specific interactions that regulate cell-cell adhesion, the immune response, microbial pathogenesis, and other cellular events. Many cell surface markers were discovered and identified as glycoconjugates such as stage-specific embryonic antigen, Tra-1-60/81 and various other cell surface molecules (e...
December 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/29039121/degradation-of-glycosphingolipids-in-oyster-ceramide-glycanase-and-ceramidase-in-the-hepatopancreas-of-oyster-crassostrea-virginica
#11
Nadejda V Pavlova, Su-Chen Li, Yu-Teh Li
The hepatopancreas of oyster, Crassostrea virginica, was found to contain two unique glycosphingolipid (GSL) cleaving enzymes, ceramide glycanase (CGase) and ceramidase. These two enzymes were found to be tightly associated together through the consecutive purification steps including gel filtration, hydrophobic interaction and cation-exchange chromatographies. They were separated only by preparatory SDS-PAGE. The purified CGase was found to have a molecular mass of 52 kDa and pH optimum of 3.2-3.3. This enzyme prefers to hydrolyze the acidic GSLs, II(3)SO3LacCer and gangliosides over the neutral GSLs...
October 16, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28980094/podocalyxin-as-a-major-pluripotent-marker-and-novel-keratan-sulfate-proteoglycan-in-human-embryonic-and-induced-pluripotent-stem-cells
#12
REVIEW
Hidenao Toyoda, Yuko Nagai, Aya Kojima, Akiko Kinoshita-Toyoda
Podocalyxin (PC) was first identified as a heavily sialylated transmembrane protein of glomerular podocytes. Recent studies suggest that PC is a remarkable glycoconjugate that acts as a universal glyco-carrier. The glycoforms of PC are responsible for multiple functions in normal tissue, human cancer cells, human embryonic stem cells (hESCs), and human induced pluripotent stem cells (hiPSCs). PC is employed as a major pluripotent marker of hESCs and hiPSCs. Among the general antibodies for human PC, TRA-1-60 and TRA-1-81 recognize the keratan sulfate (KS)-related structures...
October 5, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28971282/investigating-the-candidacy-of-the-serotype-specific-rhamnan-polysaccharide-based-glycoconjugates-to-prevent-disease-caused-by-the-dental-pathogen-streptococcus-mutans
#13
Frank St Michael, Qingling Yang, Chantelle Cairns, Evgeny Vinogradov, Perry Fleming, Alexander C Hayes, Annie Aubry, Andrew D Cox
Dental caries remains a major health issue and the Gram-positive bacterium Streptococcus mutans is considered as the major pathogen causing caries. More recently, S. mutans has been recognised as a cause of endocarditis, ulcerative colitis and fatty acid liver disease along with the likelihood of increased cerebral hemorrhage following a stroke if S. mutans is present systemically. We initiated this study to examine the vaccine candidacy of the serotype specific polysaccharides elaborated by S. mutans. We have confirmed the carbohydrate structures for the serotype specific rhamnan containing polysaccharides from serotypes c, f and k...
October 2, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28823097/epitope-mapping-of-histo-blood-group-antigens-bound-to-norovirus-vlps-using-std-nmr-experiments-reveals-fine-details-of-molecular-recognition
#14
Brigitte Fiege, Mila Leuthold, Francisco Parra, Kevin P Dalton, Peter J Meloncelli, Todd L Lowary, Thomas Peters
Attachment of human noroviruses to histo blood group antigens (HBGAs) is thought to be critical for the infection process. Therefore, we have determined binding epitopes of synthetic type 1 to 6 blood group A- and B-tetrasaccharides binding to GII.4 human Norovirus virus like particles (VLPs) using STD NMR experiments. So far, little information is available from crystal structure analysis studies on the interactions of the reducing-end sugars with the protruding domain (P-domain) of the viral coat protein VP1...
October 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28823068/stress-affects-surface-glycoconjugates-of-the-rat-endometrium-at-the-time-of-implantation
#15
Elham Aliabadi, Zohreh Makoolati, Tahereh Talaei-Khozani, Fakhreddin Mesbah Ardekani
One of the treatments to infertility is In Vitro Fertilization (IVF). In the course of IVF, fertilization rate can be improved through stress reduction. Probably one of the causes of low outcome of IVF is changes in uterine glycoconjugates that are first site of contact between blastocyst and uterus, due to stress, e.g., stress of injection. Thus, the study of the injectional stress effects on implantation period is very important to improve the outcome of IVF. Sixteen mature female rats were divided to experimental and control groups...
October 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28822024/glycosaminoglycans-and-glycolipids-as-potential-biomarkers-in-lung-cancer
#16
Guoyun Li, Lingyun Li, Eun Ji Joo, Ji Woong Son, Young Jin Kim, Jae Ku Kang, Kyung Bok Lee, Fuming Zhang, Robert J Linhardt
In this report, we used liquid chromatography-mass spectrometry and Western blotting to analyze the content and structure of glycosaminoglycans, glycolipids and selected proteins to compare differences between patient-matched normal and cancerous lung tissues obtained from lung cancer patients. The cancer tissue samples contained over twice as much chondroitin sulfate (CS)/dermatan sulfate (DS) as did the normal tissue samples, while the amount of heparan sulfate (HS) and hyaluronan (HA) in normal and cancer tissues were not significantly different...
October 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28812216/an-overview-of-in-vitro-and-in-vivo-glycation-of-albumin-a-potential-disease-marker-in-diabetes-mellitus
#17
REVIEW
Km Neelofar, Jamal Ahmad
Non-enzymatic glycation of macromolecules, especially proteins leading to their oxidation is increased in diabetes mellitus due to hyperglycaemia and play an important role in associated complications of the disease. Protein glycation mostly occurs in intra chain lysine residues resulting in the formation of early stage Amadori products which are finally converted to advance glycation end products (AGEs). This review deals with the structural studies of in vitro and in vivo glycated human serum albumin (HSA)...
October 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28808804/identification-of-a-new-liver-specific-c-type-mrna-transcriptional-variant-for-mouse-st3gal5-gm3-gm4-synthase
#18
Fumi Shishido, Satoshi Uemura, Takahiro Nitta, Jin-Ichi Inokuchi
GM3, a major lipid component of the plasma membrane outer leaflet in mammalian cells, is synthesized in the luminal side of Golgi by ST3GAL5 protein (ST3G5), a type II membrane protein. Two strains of St3Gal5 knockout mice have been established for studies of GM3 physiological function: St3Gal5-Ex5-KO (lacking exon 5, which contains the catalytic domain of ST3G5), and St3Gal5-Ex3-KO (lacking exon 3, which contains the initiation codons). Results of the present study demonstrate that GM3 synthesis is still present, at a low level, in liver of St3Gal5-Ex3-KO mice...
October 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28752383/construction-and-functional-characterization-of-truncated-versions-of-recombinant-keratanase-ii-from-bacillus-circulans
#19
Haisheng Wang, Wenqin He, Peixia Jiang, Yanlei Yu, Lei Lin, Xiaojun Sun, Mattheos Koffas, Fuming Zhang, Robert J Linhardt
There is a need for degradative enzymes in the study of glycosaminoglycans. Many of these enzymes are currently available either in their natural or recombinant forms. Unfortunately, progress in structure-activity studies of keratan sulfate (KS) have been impeded by the lack of a commercially available endo-β-N-acetylglucosaminidase, keratantase II. The current study uses a recently published sequence of a highly thermostable keratanase II identified in Bacillus circulans to clone and express a series of truncation mutants in Escherichia coli BL21...
October 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28752382/glyco-24-xxiv-international-symposium-on-glycoconjugates
#20
(no author information available yet)
No abstract text is available yet for this article.
October 2017: Glycoconjugate Journal
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