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Glycoconjugate Journal

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https://www.readbyqxmd.com/read/28725972/the-versatility-of-n-alkyl-methoxyamine-bi-functional-linkers-for-the-preparation-of-glycoconjugates
#1
Stefan Munneke, Emma M Dangerfield, Bridget L Stocker, Mattie S M Timmer
The application of N-glycosyl-N-alkyl-methoxyamine bi-functional linkers for the synthesis of a variety of glycoconjugates is described. The linker contains a specific functional group, such as an amine, azide, thiol, or carboxylic acid, which can be used for conjugation methodologies that include amide ligation, sulfonylation, copper-mediated Huisgen cycloaddition or thiol-maleimide coupling. In this way, glycoconjugates equipped with biotin, a fluorescent reporter, or a protein were efficiently synthesised, thus demonstrating the versatility of this type of oxyamine linker for the construction of glycoconjugate probes...
July 19, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28676909/unexpected-features-of-exponentially-growing-tobacco-bright-yellow-2-cell-suspension-culture-in-relation-to-excreted-extracellular-polysaccharides-and-cell-wall-composition
#2
Mohammad Issawi, Mohammad Muhieddine, Celine Girard, Vincent Sol, Catherine Riou
This article presents a new insight about TBY-2 cells; from extracellular polysaccharides secretion to cell wall composition during cell suspension culture. In the medium of cells taken 2 days after dilution (end of lag phase), a two unit pH decrease from 5.38 to 3.45 was observed and linked to a high uronic acid (UA) amount secretion (47.8%) while, in 4 and 7 day-old spent media, pH increased and UA amounts decreased 35.6 and 42.3% UA, respectively. To attain deeper knowledge of the putative link between extracellular polysaccharide excretion and cell wall composition, we determined cell wall UA and neutral sugar composition of cells from D2 to D12 cultures...
July 4, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28674831/quantification-and-comparison-of-acidic-polysaccharides-in-edible-fish-intestines-and-livers-using-hplc-ms-ms
#3
Shuang Song, Qi Yu, Bao Zhang, Chunqing Ai, Yujiao Sun, Yinghuan Fu, Meiyu Zhao, Chengrong Wen
Fish intestines and livers are usually considered as delicious and nutritious food in China. Acidic polysaccharides are important nutrients in these food of animal origin, but there is currently little information regarding their quantitative distributions. The present study demonstrated a method to quantify acidic polysaccharides simultaneously by analyzing their disaccharides produced from the acid hydrolysis using high-performance liquid chromatography (HPLC) coupled with triple quadrupole mass spectrometry...
July 3, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28656506/comparing-naturally-occurring-glycosylated-forms-of-proline-rich-antibacterial-peptide-drosocin
#4
Deepti S Lele, Gagandeep Kaur, Menithalaxmi Thiruvikraman, Kanwal J Kaur
Antimicrobial peptides (AMPs) are key players of innate immunity. Amongst various classes of AMPs, proline rich AMPs from insects enjoy special attention with few members of this class bearing O-glycosylation as post-translational modification. Drosocin, a 19 amino acid glycosylated AMP is a member of proline rich class, synthesized in the haemolymph of Drosophila melanogaster upon bacterial challenge. We report herein the chemical synthesis of drosocin carrying disaccharide (β-Gal(1 → 3)α-GalNAc) and comparison of its structural and functional properties with another naturally occurring monoglycosylated form of drosocin i...
June 27, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28597244/seasonal-and-flight-related-variation-of-galectin-expression-in-heart-liver-and-flight-muscles-of-yellow-rumped-warblers-setophaga-coronata
#5
Stefanie S Bradley, Morag F Dick, Christopher G Guglielmo, Alexander V Timoshenko
Galectins, a family of multifunctional glycan-binding proteins, are proposed as biomarkers of cellular stress responses. Avian migration is an energetically challenging physical stress, which represents a physiological model of muscular endurance exercises. This study assesses change in galectin gene expression profiles associated with seasonal variation in migratory state and endurance flight in yellow-rumped warblers (Setophaga coronata). Bioinformatics analysis and real-time qPCR were used to analyse the expression of galectins in flight muscle, heart and liver tissues of 15 warblers separated into three groups of winter unflown, and fall migratory flown/unflown birds...
June 8, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28577071/isolation-of-a-methylated-mannose-binding-protein-from-terrestrial-worm-enchytraeus-japonensis
#6
Shigeru Ogawa, Mamoru Mizuno, Mayuko Suzuki, Kohtaro Goto, Yuriko Hirose, Akio Matsuda, Takuya Saito, Suguru Oguri, Kiyoshi Furukawa
To elucidate a biological role of the methylated mannose residues found in N-glycans of terrestrial worm Enchytraeus japonensis, we first synthesized 3-O-methyl mannose- and 4-O-methyl mannose-derivatives and immobilized them to Sepharose 4B beads in order to isolate the sugar-binding protein. When whole protein extracts from the worms was applied to a series of the columns immobilized with the modified and unmodified mannose-derivatives, respectively, a protein with a molecular weight of 25,000 was isolated by 4-O-methyl mannose-immobilized column chromatography, and termed as a methylated mannose-binding protein (mMBP)...
June 2, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28624993/cancer-stem-cell-marker-glycosylation-nature-function-and-significance
#7
REVIEW
Brody W Mallard, Joe Tiralongo
Glycans are essential for the maintenance of normal biological function, with alterations in glycan expression being a hallmark of cancer. Cancer stem cells (CSCs) are a subset of cells within a tumour capable of self-renewal, cellular differentiation and resistances to conventional therapies. As is the case with stem cells, marker proteins present on the cell surface are frequently used to identify and enrich CSCs, with the expression of these markers statistical correlating with the likelihood of cancer recurrence and overall patient survival...
August 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28616799/dolichol-phosphate-mannose-synthase-a-glycosyltransferase-with-unity-in-molecular-diversities
#8
REVIEW
Dipak K Banerjee, Zhenbo Zhang, Krishna Baksi, Jesús E Serrano-Negrón
N-glycans provide structural and functional stability to asparagine-linked (N-linked) glycoproteins, and add flexibility. Glycan biosynthesis is elaborative, multi-compartmental and involves many glycosyltransferases. Failure to assemble N-glycans leads to phenotypic changes developing infection, cancer, congenital disorders of glycosylation (CDGs) among others. Biosynthesis of N-glycans begins at the endoplasmic reticulum (ER) with the assembly of dolichol-linked tetra-decasaccharide (Glc3Man9GlcNAc2-PP-Dol) where dolichol phosphate mannose synthase (DPMS) plays a central role...
August 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28597243/probing-the-catalytic-site-of-rabbit-muscle-glycogen-phosphorylase-using-a-series-of-specifically-modified-maltohexaose-derivatives
#9
Makoto Nakamura, Yasushi Makino, Chika Takagi, Tohru Yamagaki, Masaaki Sato
Glycogen phosphorylase (GP) is an allosteric enzyme whose catalytic site comprises six subsites (SG1, SG-1, SG-2, SG-3, SG-4, and SP) that are complementary to tandem five glucose residues and one inorganic phosphate molecule, respectively. In the catalysis of GP, the nonreducing-end glucose (Glc) of the maltooligosaccharide substrate binds to SG1 and is then phosphorolyzed to yield glucose 1-phosphate. In this study, we probed the catalytic site of rabbit muscle GP using pyridylaminated-maltohexaose (Glcα1-4Glcα1-4Glcα1-4Glcα1-4Glcα1-4GlcPA, where GlcPA = 1-deoxy-1-[(2-pyridyl)amino]-D-glucitol]; abbreviated as PA-0) and a series of specifically modified PA-0 derivatives (Glc m -AltNAc-Glc n -GlcPA, where m + n = 4 and AltNAc is 3-acetoamido-3-deoxy-D-altrose)...
August 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28573337/immunological-characterization-of-a-rigid-%C3%AE-tn-mimetic-on-murine-inkt-and-human-nk-cells
#10
Silvia Fallarini, Alvaro Brittoli, Michele Fiore, Grazia Lombardi, Olivier Renaudet, Barbara Richichi, Cristina Nativi
The ability of a rigid α-Tn mimetic (compound 1) to activate murine invariant natural killer T (iNKT) and human natural killer (NK) cells, two subsets of lymphocytes involved in cancer immunesurveillance, was investigated. For this purpose, the mimetic 1 was properly conjugated to a stearic acid containing glycerol-based phospholipid (compound 5) to be presented, in the context of the conserved non polymorphic major histocompatibility complex class I-like molecules (CD1d), to iNKT cells. On the contrary, the mimetic 1 was conjugated to a multivalent peptide-based scaffold (compound 6) to induce NK cell activation...
August 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28555433/structural-and-activity-variability-of-fractions-with-different-charge-density-and-chain-length-from-pharmaceutical-heparins
#11
Yanlei Yu, Makoto Hirakane, Daisuke Mori, Lei Lin, Fuming Zhang, Hong Zhang, Robert J Linhardt
Heparin is a structurally complex polysaccharide used as a clinical anticoagulant. It is comprised of a heterogeneous mixture of polysaccharide chains having a variety of sequences and lengths. The production methods and regulatory controls of pharmaceutical heparins have changed over the years. This study assesses the structural and activity uniformity of the polysaccharide chains comprising two contemporary heparin products. The heparin fractions with different sizes and charges were separated with size exclusion and ion exchange chromatography...
August 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28523604/chemo-enzymatic-synthesis-of-a-glycosylated-peptide-containing-a-complex-n-glycan-based-on-unprotected-oligosaccharides-by-using-dmt-mm-and-endo-m
#12
Yusuke Tomabechi, Toshihiko Katoh, Munetaka Kunishima, Toshiyuki Inazu, Kenji Yamamoto
For chemo-enzymatic synthesis of a glycosylated peptide, 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) was used for the synthesis of a N-acetylglucosaminyl peptide and a pseudoglycopeptide by solid-phase peptide synthesis without the requirement of protecting groups on the carbohydrate. We also performed transglycosylation of an N-glycan to the N-acetylglucosaminyl peptide using endo-β-N-acetylglucosaminidase from Mucor hiemalis (Endo-M) to synthesize a glycopeptide containing a complex N-glycan...
August 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28493026/structural-and-functional-characterization-of-a-novel-immunomodulatory-glycoprotein-isolated-from-ajowan-trachyspermum-ammi-l
#13
R R Shruthi, Y P Venkatesh, G Muralikrishna
Ajowan (Trachyspermum ammi L.) spice has been used in food preparations and also as a traditional medicine in Ayurveda. Although a number of pharmacological activities have been attributed to ajowan, its role in immunomodulation is not known. The main objective of the present study is to examine the macromolecular immunomodulatory components. Macrophage activation was studied by nitric oxide (NO) release, phagocytosis and secretion of pro-inflammatory cytokines as the markers. Ethanol precipitate (fractional) of ajowan aqueous extract was subjected to conventional chromatography (Q Sepharose followed by Bio-Gel P-100)...
August 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28455724/reevaluation-of-pholiota-squarrosa-lectin-reactive-haptoglobin-as-a-pancreatic-cancer-biomarker-using-an-improved-elisa-system
#14
Ken Kusama, Yuki Okamoto, Keiko Saito, Tsukasa Kasahara, Teizo Murata, Yasushi Ueno, Yuka Kobayashi, Yoshihiro Kamada, Eiji Miyoshi
An increase in Lewis- and core-type fucosylation of haptoglobin has been reported in patients with pancreatic cancer (PC), suggesting that fucosylated haptoglobin is a candidate PC biomarker. Previously, we developed a Pholiota squarrosa lectin antibody enzyme-linked immunosorbent assay (PhoSL-ELISA) system for the detection of core-fucosylated haptoglobin. However, with this methodology, positive results were only obtained for some patients with PC, demonstrating the need for a more sensitive detection system...
August 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28401373/sulfated-glycosaminoglycans-in-protein-aggregation-diseases
#15
REVIEW
Kazuchika Nishitsuji, Kenji Uchimura
Protein aggregation diseases are characterized by intracellular or extracellular deposition of misfolded and aggregated proteins. These aggregated deposits contain multiple proteinaceous and non-protein components that are thought to play critical roles in the etiology and pathogenesis of protein aggregation diseases in vivo. One of these components, the sulfated glycosaminoglycans (GAGs), includes heparan sulfate, chondroitin sulfate, and keratan sulfate. The sulfated GAGs are negatively charged heteropolysaccharides expressed in all mammalian tissues...
August 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28389847/a-pilot-study-of-salivary-n-glycome-in-hbv-induced-chronic-hepatitis-cirrhosis-and-hepatocellular-carcinoma
#16
Yannan Qin, Yaogang Zhong, Tianran Ma, Jiaxu Zhang, Ganglong Yang, Feng Guan, Zheng Li, Baozhen Li
Hepatitis B is a potentially life-threatening liver infection caused by the hepatitis B virus (HBV), which can lead to chronic liver disease and put people at high risk of death from cirrhosis of the liver and liver cancer. However, little is known about the correlation of salivary N-linked glycans related to HBV-infected liver diseases. Here we investigated N-linked glycome in saliva from 200 subjects (50 healthy volunteers (HV), 40 HBV-infected patients (HB), 50 cirrhosis patients (HC), and 60 hepatocellular carcinoma patients (HCC) using MALDI-TOF/TOF-MS...
August 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28349379/ulva-pertusa-lectin-1-delivery-through-adenovirus-vector-affects-multiple-signaling-pathways-in-cancer-cells
#17
Gongchu Li, Zhenzhen Zhao, Bingbing Wu, Qunshu Su, Liqin Wu, Xinyan Yang, Jing Chen
Ulva pertusa lectin 1 (UPL1) is a N-acetyl-D-glucosamine (GlcNAc) binding lectin in marine green alga Ulva pertusa. Exogenous UPL1 colocalized with protein arginine methyltransferase 5 (PRMT5), methylosome protein 50 (MEP50), β-actin and β-tubulin, indicating the interaction of UPL1 with the methylosome and cytoskeleton. UPL1 delivery through adenovirus vector (Ad-UPL1) dramatically induced extracellularly regulated protein kinases 1/2 (ERK1/2) phosphorylation in liver cancer cell lines BEL-7404 and Huh7...
August 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28299519/insights-into-the-effects-of-glycosylation-and-the-monosaccharide-binding-activity-of-the-plant-lectin-cratabl
#18
Laercio Pol-Fachin
CrataBL is a glycoprotein isolated from Crataeva tapia bark, containing two N-glycosylation sites. It has been identified to present lectin activity with some specificity for binding glucose over galactose. However, to date, no information on the effects of glycosylation or CrataBL monosaccharide-binding sites and monosaccharide specificity has been obtained. Thus, molecular docking and molecular dynamics simulations were employed to characterize the glycosylated CrataBL conformation and dynamics in aqueous solutions, as well as the molecular basis for its binding specificity...
August 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28577070/heparan-sulfate-proteoglycans-as-key-regulators-of-the-mesenchymal-niche-of-hematopoietic-stem-cells
#19
Dulce Papy-Garcia, Patricia Albanese
The complex microenvironment that surrounds hematopoietic stem cells (HSCs) in the bone marrow niche involves different coordinated signaling pathways. The stem cells establish permanent interactions with distinct cell types such as mesenchymal stromal cells, osteoblasts, osteoclasts or endothelial cells and with secreted regulators such as growth factors, cytokines, chemokines and their receptors. These interactions are mediated through adhesion to extracellular matrix compounds also. All these signaling pathways are important for stem cell fates such as self-renewal, proliferation or differentiation, homing and mobilization, as well as for remodeling of the niche...
June 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28265838/glycosaminoglycans-major-biological-players
#20
EDITORIAL
Sylvie Ricard-Blum
No abstract text is available yet for this article.
June 2017: Glycoconjugate Journal
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