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Glycoconjugate Journal

Bridget L Stocker, Kristel Kodar, Kanu Wahi, Amy J Foster, Jacquie L Harper, Daiki Mori, Sho Yamasaki, Mattie S M Timmer
Trehalose glycolipids (TGLs) are promising vaccine adjuvants, however effects of glycolipid presentation in the in vitro evaluation, and ultimate selection, of lead vaccine adjuvants are often overlooked. To this end, we synthesised a variety of TGLs and determined how the physicochemical presentation of these lipids influenced the cytokine response by bone marrow derived macrophages (BMMs). The TGLs were presented to wild-type and Mincle-/- BMMs as micellar solutions, coated on plates, coated on beads or surfactant solubilised...
January 8, 2019: Glycoconjugate Journal
Sumeyye Cavdarli, Justine H Dewald, Nao Yamakawa, Yann Guérardel, Mickaël Terme, Jean-Marc Le Doussal, Philippe Delannoy, Sophie Groux-Degroote
Mainly restricted to the nervous system in healthy adults, complex gangliosides such as GD3 and GD2 have been shown to be involved in aggressiveness and metastasis of neuro-ectoderm derived tumors such as melanoma and neuroblastoma. Interestingly, O-acetylated forms of GD2, not expressed in human peripheral nerve fibers, are highly expressed in GD2+ tumor cells. Very little information is known regarding the expression of O-acetylated disialogangliosides in breast cancer (BC) cell lines. Here, we analyzed the expression of GD2, GD3 and their O-acetylated forms O-acetyl-GD2 (OAcGD2) and O-acetyl-GD3 (OAcGD3) in BC cells...
January 5, 2019: Glycoconjugate Journal
Genny Degani, Alberto Barbiroli, Paula Magnelli, Stefania Digiovanni, Alessandra Altomare, Giancarlo Aldini, Laura Popolo
Advanced glycation end products (AGEs) and advanced lipoxidation end products (ALEs), resulting from non-enzymatic modifications of proteins, are potentially harmful to human health. They directly act on proteins, affecting structure and function, or through receptor-mediated mechanisms. RAGE, a type I transmembrane glycoprotein, was identified as a receptor for AGEs. RAGE is involved in chronic inflammation, oxidative stress-based diseases and ageing. The majority of RAGE ligands bind to the VC1 domain. This domain was successfully expressed and secreted by Pichia pastoris...
January 5, 2019: Glycoconjugate Journal
Melissa Baerenfaenger, Manuela Moritz, Bernd Meyer
Significant changes of glycan structures are observed in humans if diseases like cancer, arthritis or inflammation are present. Thus, interest in biomarkers based on glycan structures has rapidly emerged in recent years and monitoring disease specific changes of glycosylation and their quantification is of great interest. Mass spectrometry is most commonly used to characterize and quantify glycopeptides and glycans liberated from the glycoprotein of interest. However, ionization properties of glycopeptides can strongly depend on their composition and can therefore lead to intensities that do not reflect the actual proportions present in the intact glycoprotein...
January 5, 2019: Glycoconjugate Journal
Tania M Cortázar, Iain B H Wilson, Alba Hykollari, Edgar A Reyes, Nohora A Vega
We regret to inform of an incomplete affiliation in this article.
January 4, 2019: Glycoconjugate Journal
Yanxia Jin, Yajun Yang, Yanting Su, Xiangdong Ye, Wei Liu, Qing Yang, Jie Wang, Xiangning Fu, Yongsheng Gong, Hui Sun
Non-small cell lung cancer (NSCLC) is a malignant tumor with high morbidity and mortality. The clinical biomarkers currently used for the early diagnosis of lung cancer have poor sensitivity and specificity. Therefore, it is urgent to identify sensitive biomarkers for the early detection of NSCLC to improve the patient survival of patients. In our previously study, we identified glycoprotein alpha-1-antichymotrypsin (AACT) as an early biomarker of NSCLC. In this study, serum glycopeptides were enriched using the high-GlcNAc-specific binding lectin, AANL/AAL2, for further quantitative proteomics analysis using LC-MS/MS...
January 3, 2019: Glycoconjugate Journal
Yosuke Yamada, Makoto Harada, Koji Hashimoto, Ran Guo, Takero Nakajima, Toshihide Kashihara, Mitsuhiko Yamada, Toshifumi Aoyama, Yuji Kamijo
Serum sulfatides are critical glycosphingolipids that are present in lipoproteins and exert anticoagulant effects. A previous study reported decreased levels of serum sulfatides in hemodialysis patients and suggested an association with cardiovascular disease. However, the mechanism of changes in serum sulfatides in chronic kidney dysfunction has not been well investigated. The current study examined whether a chronic kidney disease (CKD) state could decrease serum sulfatide levels using 5/6 nephrectomy (5/6NCKD) mice, an established CKD murine model, and studied the mechanisms contributing to diminished sulfatides...
December 10, 2018: Glycoconjugate Journal
Terry Nguyen-Khuong, Alexander Pralow, Udo Reichl, Erdmann Rapp
Analysis of glycans via a porous graphitized carbon liquid chromatography (PGC-LC) coupled with electrospray ionization (tandem) mass spectrometry (ESI-MS(/MS)) is a powerful analytical method in the field of glycomics. Isobaric glycan structures can be identified reliably with the help of PGC-LC separation and subsequent identification by ESI-MS(/MS) in negative ion mode. In an effort to adapt PGC-LC-ESI-MS(/MS) to the nano-scale operation, spray instability along the nano-PGC-LC gradient was repeatedly observed on an LTQ Orbitrap Elite mass spectrometer equipped with a standard nano-electrospray ionization source...
December 2018: Glycoconjugate Journal
Yuri Mineguchi, Midori Miyoshi, Epi Taufik, Ayumi Kawamura, Takuya Asakawa, Isao Suzuki, Kousaku Souma, Michiko Okubo, Tadao Saito, Kenji Fukuda, Sadaki Asakuma, Tadasu Urashima
Mammalian milk/colostrum usually contains oligosaccharides along with the predominant disaccharide lactose. It has been found that the number and identity of these milk oligosaccharides varies among mammalian species. Oligosaccharides predominate over lactose in the milk/colostrum of Arctoidea species (Carnivora), whereas lactose predominates over milk oligosaccharides in Artiodactyla including cow, sheep, goat, camel, reindeer and pig. To clarify whether heterogeneity of a variety of milk oligosaccharides is found within other species of Artiodactyla, they were studied in the milk of giraffe, sitatunga, deer and water buffalo...
December 2018: Glycoconjugate Journal
Wenhong Zhao, Hui Chen, Ligen Wu, Weibin Ma, Yanli Xie
In this study, four main components of feruloylated oligosaccharides (FOs),FOs-1, FOs-2, FOs-3 and FOs-4,were isolated from wheat bran by use of Amberlite XAD-2 and Sephadex LH-20. Structural characterization of FOs was determined by use of high performance liquid chromatography (HPLC), gas chromatography (GC) and fourier transform-infrared spectroscopy (FT-IR). Antioxidant properties were investigated in vitro. Average degrees of polymerization (DP) of the four components (FOs-1, FOs-2, FOs-3 and FOs-4) were approximately 10...
December 2018: Glycoconjugate Journal
Chunyang Cao, Shuang Song, Bin Liu, Chunqing Ai, Zhenjun Zhu, Lilong Wang, Sufeng Wu
Acidic polysaccharides are attractive functional ingredients in shellfish which are consumed as delicious and nutritious foods world widely. In the present study, acidic polysaccharides from 21 species of edible shellfish were characterized and quantified by analyzing their repeated disaccharides using the multiple reaction monitoring (MRM) mode of triple quadrupole mass spectrometer upon acid degradation and 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization. A total of 6 glycosaminoglycans (GAGs) and 8 non-GAGs with repeated disaccharide units of a hexuronic acid linked to a hexosamine or a hexose were detected...
December 2018: Glycoconjugate Journal
Mamta Sharma, Prajna Hegde, Kavita Hiremath, Vishwanath Reddy H, A S Kamalanathan, Bale M Swamy, Shashikala R Inamdar
Plant lectins are gaining interest because of their interesting biological properties. Several Adenia species, that are being used in traditional medicine to treat many health ailments have shown presence of lectins or carbohydrate binding proteins. Here, we report the purification, characterization and biological significance of N-Acetyl galactosamine specific lectin from Adenia hondala (AHL) from Passifloraceae family. AHL was purified in a single step by affinity chromatography on asialofetuin Sepharose 4B column, characterized and its fine sugar specificity determined by glycan array analysis...
December 2018: Glycoconjugate Journal
Sulav Sharma, Yan Ding, Ken F Jarrell, Inka Brockhausen
Archaea are ubiquitous single-cell microorganisms that have often adapted to harsh conditions and play important roles in biogeochemical cycles with potential applications in biotechnology. Methanococcus maripaludis, a methane-producing archaeon, is motile through multiple archaella on its cell surface. The major structural proteins (archaellins) of the archaellum are glycoproteins, modified with N-linked tetrasaccharides that are essential for the proper assembly and function of archaella. The aglW gene, encoding the putative 4-epimerase AglW, plays a key role in the synthesis of the tetrasaccharide...
December 2018: Glycoconjugate Journal
Ayaka Noda, Miki Kato, Shota Miyazaki, Mamoru Kyogashima
We introduce the principle of a new technique to isolate glycosphingolipids (GSLs) from phospholipids. Neutral and acidic GSLs in organic solvent bind to titanium dioxide under neutral pH and can be eluted with 5 mg/ml of 2,5-dihydroxybenzoic acid in methanol. This special property is applicable for eliminating phospholipids, including sphingomyelin, which cannot be eliminated by a typical mild alkaline treatment. By using this technique, we demonstrated the rapid separation of minor components of GSLs, namely sulfatide and gangliosides from rabbit serum and liver, respectively...
December 2018: Glycoconjugate Journal
Tripti Raghavendra, Saniya Patil, Raju Mukherjee
Peptidoglycan, a major glycoconjugate in the mycobacterial cell envelope provides strength to resist osmotic stress and plays a pivotal role in maintaining the cellular morphology. Several unique growth stage specific structural alterations occur in its constituent monosaccharides and peptides that allow Mycobacterium to survive nutrient starvation and environmental stress. Here, we discuss the enzymes involved in its intricate biosynthesis that are novel targets for therapeutic intervention and provide an opportunity for potential antibiotic adjuvants...
October 2018: Glycoconjugate Journal
Yasunobu Yoshida, Jun-Ichi Furukawa, Shoichi Naito, Kenichi Higashino, Yoshito Numata, Yasuro Shinohara
Hepatocellular carcinoma (HCC) is the major subtype of primary liver cancer, and is typically diagnosed late in its course. Considering the limitations and the reluctance of patients to undergo a liver biopsy, a reliable, noninvasive diagnostic marker that predicts and assesses the treatment and prognosis of HCC is needed. With recent technological advances of mass spectrometry, glycomics is gathering momentum and holds substantial potential to discover new glycan markers in cancer research. Here, to discover specific glycan markers for the early diagnosis of HCC, we analyzed the glycan profiles of gel-separated serum proteins of progressive liver disease model mice...
October 2018: Glycoconjugate Journal
Seanghai Hor, Takumi Kodama, Nobuo Sugiura, Hikaru Kondou, Mio Yanagida, Keiya Yanagisawa, Aoki Shibasawa, Bunta Tsuzuki, Naoto Fukatsu, Kazuya Nagao, Kenji Yamana, Kazuya I P J Hidari, Hideto Watanabe, Osami Habuchi, Hirofumi Nakano
Chondroitin sulfate E (CS-E) plays a crucial role in diverse processes ranging from viral infection to neuroregeneration. Its regiospecific sulfation pattern, generated by N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase (GalNAc4S-6ST), is the main structural determinant of its biological activity. Inhibitors of GalNAc4S-6ST can serve as powerful tools for understanding physiological functions of CS-E and its potential therapeutic leads for human diseases. A family of new 4-acylamino-β-GalNAc derivatives and 4-azido-β-GalNAc derivatives were synthesized for their potential application as inhibitors of GalNAc4S-6ST...
October 2018: Glycoconjugate Journal
Shyamasree Ghosh
Sialic acids are negatively charged nine carbon monosaccharides located terminally on glycoproteins and glycolipids that control cellular physiological processes. Sialylation is a post translational modification (ptm) regulated by enzymes and has been studied in prokaryotes including bacteria, dueterostomes including vertebrates, Cephalochordates, Ascidians, Echinoderms and protostomes including Molluscs and Arthropods and Plant. Although diverse structures of sialylated molecules have been reported in different organisms, unravelling sialylation in insect biology is a completely new domain...
October 2018: Glycoconjugate Journal
Guiqiang Zhang, Peiyuan Jia, Hongtao Liu, Tao Hu, Yuguang Du
Porcine circovirus type 2 (PCV2)-associated diseases have led to huge economic losses in pig industry. Our laboratory previously found that conjugation of chitosan oligosaccharides (COS) enhanced the immunogenicity of PCV2 vaccine against infectious pathogens. In this study, an effective adjuvant system was developed by covalent conjugation of COS via a carrier protein (Ovalbumin, OVA) to further increase the immunogenicity of vaccine. Its effect on dendritic cells maturation was assessed in vitro and its immunogenicity was investigated in mice...
October 2018: Glycoconjugate Journal
Monika Franczyk, Jacek Wawrzykowski, Marta Kankofer
Decorin is a small leucine-rich proteoglycan which is involved in multiple biological functions mainly as a structural and signaling molecule. Due to its biological properties in connective tissue, decorin may participate in remodeling of ECM during attachment and detachment of placenta within the course of pregnancy and at parturition in cows. The aim of the study was to detect the presence of decorin protein in bovine placental tissues and to evaluate its profile during pregnancy and at parturition. Placental tissues from healthy pregnant cows (2-5 month) were collected in abattoir (n = 10), while parturient tissues were obtained during caesarian section at physiological term (n = 6)...
October 2018: Glycoconjugate Journal
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