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Food Microbiology

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https://www.readbyqxmd.com/read/28800831/characterization-of-enterococcus-durans-152-bacteriocins-and-their-inhibition-of-listeria-monocytogenes-in-ham
#1
Lihui Du, Fang Liu, Ping Zhao, Tong Zhao, Michael P Doyle
Listeria monocytogenes is a nonfastidious, widely occurring foodborne pathogen that is a major challenge to the food industry. Enterococcus durans 152, a confirmed L. monocytogenes-control microorganism, was isolated from floor drain samples from a food processing facility. In this study, the two bacteriocins produced by E. durans 152 were characterized and identified as Dur 152A (an enterocin L50A derivative with two amino acid substitutions of I→M) and enterocin L50B. The bacteriocins were then partially purified and evaluated for inhibitory activity to L...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28800830/modeling-carbon-dioxide-effect-in-a-controlled-atmosphere-and-its-interactions-with-temperature-and-ph-on-the-growth-of-l-%C3%A2-monocytogenes-and-p-%C3%A2-fluorescens
#2
Olivier Couvert, Stéphanie Guégan, Bernard Hézard, Véronique Huchet, Adrienne Lintz, Dominique Thuault, Valérie Stahl
The effect of carbon dioxide, temperature, and pH on growth of Listeria monocytogenes and Pseudomonas fluorescens was studied, following a protocol to monitor microbial growth under a constant gas composition. In this way, the CO2 dissolution didn't modify the partial pressures in the gas phase. Growth curves were acquired at different temperatures (8, 12, 22 and 37 °C), pH (5.5 and 7) and CO2 concentration in the gas phase (0, 20, 40, 60, 80, 100% of the atmospheric pressure, and over 1 bar). These three factors greatly influenced the growth rate of L...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28800829/combination-of-endolysins-and-high-pressure-to-inactivate-listeria-monocytogenes
#3
Tomas J van Nassau, Christian A Lenz, Anna S Scherzinger, Rudi F Vogel
Outbreaks of listeriosis are often related to the consumption of low-processed ready-to-eat food products (e.g. soft cheeses or smoked fish) contaminated with Listeria monocytogenes. Traditional preservation techniques, such as heat treatment, cannot eliminate Listeria from these products without strongly affecting the quality of the foods. We therefore investigated the use of endolysin (PlyP40, Ply511, or PlyP825) in combination with high hydrostatic pressure processing to kill L. monocytogenes in buffer...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28800828/culture-dependent-and-culture-independent-assessment-of-spoilage-community-growth-on-vp-lamb-meat-from-packaging-to-past-end-of-shelf-life
#4
Mandeep Kaur, Hongshan Shang, Mark Tamplin, Tom Ross, John P Bowman
Packaging and storage temperature are important factors that influence the shelf-life of vacuum packed (VP) meat. In this study the shelf-life of VP bone-in lamb hind shanks stored at 8 °C and -1.2 °C was determined in parallel to analyses of starting and eventual spoilage bacterial communities via Illumina MiSeq based 16S rRNA amplicon sequencing. The mean total viable counts (TVC) and lactic acid bacterial viable counts (LAB) were observed to increase to log 7.5 CFU/cm(2) and 7 CFU/cm(2) after 6 and 42 days at 8 °C and -1...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28800827/modelling-the-effect-of-water-activity-reduction-by-sodium-chloride-or-glycerol-on-conidial-germination-and-radial-growth-of-filamentous-fungi-encountered-in-dairy-foods
#5
Nicolas Nguyen Van Long, Karim Rigalma, Louis Coroller, Robin Dadure, Stella Debaets, Jérôme Mounier, Valérie Vasseur
Water activity (aw) is one of the most influential abiotic factors affecting fungal development in foods. The effects of aw reduction on conidial germination and radial growth are generally studied by supplementing culture medium with the non-ionic solute glycerol despite food aw can also depend on the concentration of ionic solutes such as sodium chloride (NaCl). The present study aimed at modelling and comparing the effects of aw, either modified using NaCl or glycerol, on radial growth and/or conidial germination parameters for five fungal species occurring in the dairy environment...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28800826/effect-of-thyme-essential-oil-and-lactococcus-lactis-cbm21-on-the-microbiota-composition-and-quality-of-minimally-processed-lamb-s-lettuce
#6
Lorenzo Siroli, Francesca Patrignani, Diana I Serrazanetti, Pamela Vernocchi, Federica Del Chierico, Alessandra Russo, Sandra Torriani, Lorenza Putignani, Fausto Gardini, Rosalba Lanciotti
The main aim of this work was to evaluate, at pilot scale in an industrial environment, the effects of the biocontrol agent Lactococcus lactis CBM21 and thyme essential oil compared to chlorine, used in the washing step of fresh-cut lamb's lettuce, on the microbiota and its changes in relation to the time of storage. The modification of the microbial population was studied through pyrosequencing in addition to the traditional plate counts. In addition, the volatile molecule and sensory profiles were evaluated during the storage...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28800825/efficacy-evaluation-of-a-new-water-sanitizer-for-increasing-the-shelf-life-of-southern-australian-king-george-whiting-and-tasmanian-atlantic-salmon-fillets
#7
Manouchehr Khazandi, Permal Deo, Sergio Ferro, Henrietta Venter, Hongfei Pi, Simon Crabb, Tony Amorico, Abiodun D Ogunniyi, Darren J Trott
The bacterial species and specific spoilage organisms associated with the Southern Australian King George Whiting (KGW) and Tasmanian Atlantic Salmon (TAS), and the efficacy of a HOCl-containing water-based sanitization product (Electro-Chemically Activated Solution, by ECAS4) in extending the shelf life of KGW and TAS fillets were evaluated. Fillets were washed with an ECAS4 solution containing either 45 ppm or 150 ppm of free chlorine and bacterial species enumerated on selective and non-selective media, followed by identification of pure isolates by 16 S rRNA gene sequencing...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28800824/assessing-the-antimicrobial-potential-of-aerosolised-electrochemically-activated-solutions-ecas-for-reducing-the-microbial-bio-burden-on-fresh-food-produce-held-under-cooled-or-cold-storage-conditions
#8
R M S Thorn, J Pendred, D M Reynolds
The main aim of this study was to assess the antimicrobial efficacy of electrochemically activated fog (ECAF) for reducing the microbial bio-burden on artificially inoculated fresh produce held under cooled (cucumber and vine tomatoes) or cold (rocket and broccoli) storage conditions. The ECAF treatment (1100 ± 5 mV ORP; 50 ± 5 mg L(-1) free chlorine; 2.7 ± 0.1 pH) resulted in a significant log reduction in the potential pathogen E. coli recovered from rocket (2.644 Log10 CFU g(-1)), broccoli (4...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28800823/detection-and-quantification-of-alicyclobacillus-acidoterrestris-by-electrical-impedance-in-apple-juice
#9
Pilar Fernández, José Antonio Gabaldón, Mª Jesús Periago
Alicyclobacillus acidoterrestris is a thermotolerant bacterium able to grow in fruit juices and drinks, as the spoilage by Alicyclobacillus in the final product does not product any gas, but leads to a "medicine flavor" due to the formation of guaicol. Also, its detection is a challenge for the quality control departments, because it takes several days to get the results of traditional microbiology methods. This study aimed at developing a more accurate electrical impedance technique for the detection of A...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28800822/escherichia-coli-transfer-from-simulated-wildlife-feces-to-lettuce-during-foliar-irrigation-a-field-study-in-the-northeastern-united-states
#10
Daniel L Weller, Jasna Kovac, David J Kent, Sherry Roof, Jeffrey I Tokman, Erika Mudrak, Barbara Kowalcyk, David Oryang, Anna Aceituno, Martin Wiedmann
Wildlife intrusion has been associated with pathogen contamination of produce. However, few studies have examined pathogen transfer from wildlife feces to pre-harvest produce. This study was performed to calculate transfer coefficients for Escherichia coli from simulated wildlife feces to field-grown lettuce during irrigation. Rabbit feces inoculated with a 3-strain cocktail of non-pathogenic E. coli were placed in a lettuce field 2.5-72 h before irrigation. Following irrigation, the E. coli concentration on the lettuce was determined...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28800821/listeria-monocytogenes-incidence-changes-and-diversity-in-some-brazilian-dairy-industries-and-retail-products
#11
Virginie Oxaran, Sarah Hwa In Lee, Luíza Toubas Chaul, Carlos Humberto Corassin, Giovana Verginia Barancelli, Virgínia Farias Alves, Carlos Augusto Fernandes de Oliveira, Lone Gram, Elaine Cristina Pereira De Martinis
Listeria monocytogenes can cause listeriosis, a severe foodborne disease. In Brazil, despite very few reported cases of listeriosis, the pathogen has been repeatedly isolated from dairies. This has led the government to implement specific legislation to reduce the hazard. Here, we determined the incidence of L. monocytogenes in five dairies and retail products in the Southeast and Midwest regions of Brazil over eight months. Of 437 samples, three samples (0.7%) from retail and only one sample (0.2%) from the dairies were positive for L...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28800820/bacterial-diversity-of-the-colombian-fermented-milk-suero-coste%C3%A3-o-assessed-by-culturing-and-high-throughput-sequencing-and-dgge-analysis-of-16s-rrna-gene-amplicons
#12
Karina Edith Motato, Christian Milani, Marco Ventura, Francia Elena Valencia, Patricia Ruas-Madiedo, Susana Delgado
"Suero Costeño" (SC) is a traditional soured cream elaborated from raw milk in the Northern-Caribbean coast of Colombia. The natural microbiota that characterizes this popular Colombian fermented milk is unknown, although several culturing studies have previously been attempted. In this work, the microbiota associated with SC from three manufacturers in two regions, "Planeta Rica" (Córdoba) and "Caucasia" (Antioquia), was analysed by means of culturing methods in combination with high-throughput sequencing and DGGE analysis of 16S rRNA gene amplicons...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28800819/estimation-of-the-probability-of-bacterial-population-survival-development-of-a-probability-model-to-describe-the-variability-in-time-to-inactivation-of-salmonella-enterica
#13
Kento Koyama, Hidekazu Hokunan, Mayumi Hasegawa, Shuso Kawamura, Shigenobu Koseki
Despite the development of numerous predictive microbial inactivation models, a model focusing on the variability in time to inactivation for a bacterial population has not been developed. Additionally, an appropriate estimation of the risk of there being any remaining bacterial survivors in foods after the application of an inactivation treatment has not yet been established. Here, Gamma distribution, as a representative probability distribution, was used to estimate the variability in time to inactivation for a bacterial population...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28800818/characterization-of-a-novel-endolysin-lyssa11-and-its-utility-as-a-potent-biocontrol-agent-against-staphylococcus-aureus-on-food-and-utensils
#14
Yoonjee Chang, Minsik Kim, Sangryeol Ryu
Here we show that the LysSA11 endolysin, derived from the virulent Staphylococcus aureus phage SA11, has lytic activity against staphylococcal strains. Bioinformatics analysis revealed an enzymatically active CHAP (cysteine, histidine-dependent amidohydrolases/peptidases) domain at the N-terminus of LysSA11 that showed amidase activity. A novel cell wall binding domain (CBD) in the C-terminus could bind to a broad spectrum of staphylococcal cells. The bactericidal activity of LysSA11 was determined in food and utensils artificially contaminated with methicillin-resistant S...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28800817/selection-of-reference-genes-to-quantify-relative-expression-of-ochratoxin-a-related-genes-by-penicillium-nordicum-in-dry-cured-ham
#15
Victoria Bernáldez, Juan J Córdoba, María J Andrade, Alberto Alía, Alicia Rodríguez
Penicillium nordicum is an important and consistent producer of ochratoxin A (OTA) in NaCl-rich foods such as dry-cured ham. OTA is a toxic secondary metabolite which provokes negative effects on consumer health. Once OTA is produced in ham, this mycotoxin is difficult to remove. Since gene expression always precedes OTA production, analysis of expression of OTA-related genes by reverse transcription real-time PCR (RT-qPCR) could be a valuable tool to predict OTA contamination in ham. However scarce RT-qPCR protocols are properly validated leading to inconsistent data analyses...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28800816/an-investigation-of-vtx2-bacteriophage-transduction-to-different-escherichia-coli-patho-groups-in-food-matrices-and-nutrient-broth
#16
Sepo Nyambe, Catherine Burgess, Paul Whyte, Declan Bolton
This study investigated bacteriophage (phage) mediated transfer of the vtx2 gene from a donor Escherichia coli (C600φ3538(Δvtx2::cat)) to enteropathogenic (EPEC), enterotoxigenic (ETEC), enteroaggregative (EAEC), enteroinvasive (EIEC) and diffusely adherent (DAEC) E. coli strains in LB broth, milk, ground beef and lettuce. Two bacterial concentrations for both the E. coli donor and recipient strains, 3 and 5 log10 CFU/ml (LB broth and milk)/g (beef) or/cm(2) (lettuce), were used. When transductants were obtained, the location of insertion of the phage (insertion sites wrbA, yehA, sbcB, yecE and/or Z2577) in the E...
December 2017: Food Microbiology
https://www.readbyqxmd.com/read/28648298/slightly-acidic-electrolyzed-water-combined-with-chemical-and-physical-treatments-to-decontaminate-bacteria-on-fresh-fruits
#17
Charles Nkufi Tango, Imran Khan, Paul-François Ngnitcho Kounkeu, Rubab Momna, Mohammad Shakhawat Hussain, Deog-Hwan Oh
Effect of sequential combination of slightly acidic electrolyzed water (SAEW) with chemical and physical treatments on bacterial decontamination on fruits was investigated in this study. Effect of treatments on microbial and sensory quality was also analyzed after subsequent storage at 4 °C and room temperature (RT, 23 ± 0.15 °C). Whole apple and tomato fruits were inoculated with cocktail strains of Escherichia coli O157:H7 and Listeria monocytogenes. Uninoculated and inoculated fruits were washed first with distilled water (DW), calcium oxide (CaO), fumaric acid (FA), and SAEW at RT for 3 min...
October 2017: Food Microbiology
https://www.readbyqxmd.com/read/28648297/modeling-the-risk-of-salmonellosis-from-consumption-of-pistachios-produced-and-consumed-in-the-united-states
#18
Elisabetta Lambertini, Javad Barouei, Donald W Schaffner, Michelle D Danyluk, Linda J Harris
The risk of salmonellosis from consumption of pistachios produced and consumed in the U.S. was assessed through quantitative microbial risk assessment. Data on Salmonella prevalence and concentration on pistachios, nut crop volume, storage times and temperatures during processing and handling, and reductions during storage or from roasting were derived from laboratory experiments, published literature, and industry expert opinion. Uncertainty was analyzed via what-if scenarios for Salmonella prevalence, concentration, storage reduction, treatment variability, portion of crop treated, and increased consumption...
October 2017: Food Microbiology
https://www.readbyqxmd.com/read/28648296/effect-of-storage-temperature-on-the-lag-time-of-geobacillus-stearothermophilus-individual-spores
#19
Myrsini Kakagianni, Juan S Aguirre, Alexandra Lianou, Konstantinos P Koutsoumanis
The lag times (λ) of Geobacillus stearothermophilus single spores were studied at different storage temperatures ranging from 45 to 59 °C using the Bioscreen C method. A significant variability of λ was observed among individual spores at all temperatures tested. The storage temperature affected both the position and the spread of the λ distributions. The minimum mean value of λ (i.e. 10.87 h) was observed at 55 °C, while moving away from this temperature resulted in an increase for both the mean and standard deviation of λ...
October 2017: Food Microbiology
https://www.readbyqxmd.com/read/28648295/detection-and-prevalence-of-protozoan-parasites-in-ready-to-eat-packaged-salads-on-sale-in-italy
#20
Tiziana Caradonna, Marianna Marangi, Federica Del Chierico, Nicola Ferrari, Sofia Reddel, Giorgia Bracaglia, Giovanni Normanno, Lorenza Putignani, Annunziata Giangaspero
To investigate the prevalence of protozoan contamination by Giardia duodenalis, Cryptosporidium spp., Toxoplasma gondii and Cyclospora cayetanensis, in 'ready to eat' (RTE) salads on sale in Italy, 648 packages were purchased from industrial and local brands. Nine individual packages from each brand were collected per month, pooled and subjected to microscopy and molecular analyses. Microscopic examination of 864 slides detected Cryptosporidium spp. but also Blastocystis hominis and Dientamoeba fragilis. Molecular tools identified G...
October 2017: Food Microbiology
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