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Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry

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https://www.readbyqxmd.com/read/27829637/erratum-analytical-sciences-2016-vol-32-no-9-p-969-chromatographic-fingerprint-bioactivity-relationships-of-the-antitumor-properties-of-bufo-bufo-gargarizans-skin-extracts
#1
Lulu Sun, Jing Zhang, Surong Zhao, Qiong Pan, Hongmin Zhang, Hao Liu, Zhitao Zhang
On page 969, the author line,    Lulu SUN,* Jing ZHANG,** Surong ZHAO,* Qiong PAN,* Hongmin ZHANG,* Hao LIU,* and Zhitao ZHANG*should read     Lulu SUN,* Jing ZHANG,** Surong ZHAO,* Qiong PAN,* Hongmin ZHANG,* Hao LIU,*(†) and Zhitao ZHANG*(†).On page 969, the sentences,     (†) To whom correspondence should be addressed.     E-mail: liuhao6886@foxmail.com; 13956336300@126.comshould be added on the footnote.
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/27829636/announcements
#2
(no author information available yet)
No abstract text is available yet for this article.
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/27829635/quantitative-determination-of-nta-and-other-chelating-agents-in-detergents-by-potentiometric-titration-with-copper-ion-selective-electrode
#3
Sana Ito, Masaki Morita
Quantitative analysis of nitrilotriacetate (NTA) in detergents by titration with Cu(2+) solution using a copper ion selective electrode was achieved. This method tolerates a wide range of pH and ingredients in detergents. In addition to NTA, other chelating agents, having relatively lower stability constants toward Cu(2+), were also qualified with sufficient accuracy by this analytical method for model detergent formulations. The titration process was automated by automatic titrating systems available commercially...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/27829634/estimation-of-the-compatibility-of-blend-composites-of-resins-by-measuring-the-fluorescent-spectra
#4
Hideki Hayashi, Masanobu Maeda, Junpei Kuno, Taiki Naruo, Hiroki Hirota, Masaki Harada, Kouji Iida, Yukio Onouchi, Koji Hirano
The effects of adding desipramine-containing fluorescence polymer (poly(10,11-dihydro-5-[3-(N-methylamino)propyl]dibenz[b,f]azepine-2,8-diyl-alt-9,9-didodecylfluorene-2,7-diyl, PAzep-Fl) to resins were investigated. When composites were prepared by a reactive blend of poly(lactic acid) (PLA), PAzep-Fl, and diisocyanate, the molecular weight of the obtained composite was larger than that of the composite, which was blended without PAzep-Fl; this suggested that chemical bonding occured between PLA and PAzep-Fl via diisocyanate...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/27829633/a-label-free-time-resolved-luminescent-platform-for-sensitive-endonuclease-v-detection-based-on-exonuclease-iii-regulated-dna-tb-3-luminescence
#5
Huaijun Nie, Huifen Huang, Wang Li, Tao Yang
Endonuclease V (EndoV) plays the important role of nucleotide excision repair (NER) in the maintenance of genomic stability. Highly sensitive detection of EndoV was achieved through an oligonucleotides sensitizing Tb(3+) luminescent technique. We found that although both guanine-rich (G-rich) single-stranded DNA and dGMP could enhance the time-resolved luminescence of Tb(3+), their efficiencies of enhancement were considerably different. Employing such interesting phenomenon, a label-free and time-resolved luminescent strategy for the sensitive detection of EndoV activity was developed based on DNA-enhanced time-resolved luminescence (TRL) of Tb(3+)...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/27829632/quantitative-analysis-of-trace-elements-in-silicate-glass-sample-by-la-icp-qms-qms-with-an-orc-silicon-as-the-matrix-of-calibrating-solutions-and-the-internal-standard-for-measurement
#6
Yanbei Zhu
A strategy was presented for quantitative analysis of trace elements in a glass sample by laser ablation inductively coupled plasma tandem quadrupole mass spectrometry (LA-ICP-QMS/QMS) with an octapole reaction cell (ORC). Silicon in the glass was used as the internal standard as well as the matrix for making the calibrating solutions. Sample aerosols generated by the laser ablation system were introduced into the dual pass spray chamber through the make-up gas port. Calibrating solutions were nebulized into the spray chamber using a microflow nebulizer...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/27829631/a-highly-sensitive-and-selective-method-for-the-determination-of-an-iodate-in-table-salt-samples-using-malachite-green-based-spectrophotometry
#7
Mongkol Konkayan, Nunticha Limchoowong, Phitchan Sricharoen, Saksit Chanthai
A simple, rapid, and sensitive malachite green-based spectrophotometric method for the selective trace determination of an iodate has been developed and presented for the first time. The reaction mixture was specifically involved in the liberation of iodine in the presence of an excess of iodide in an acidic condition following an instantaneous reaction between the liberated iodine and malachite green dye. The optimum condition was obtained with a buffer solution pH of 5.2 in the presence of 40 mg L(-1) potassium iodide and 1...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/27829630/a-rhodamine-based-dual-chemosensor-for-the-simultaneous-detection-of-fe-3-and-cu-2
#8
Tao Lan, Feng-He Wang, Xing-Jun Xi, Chun-Wen Cheng, Wu Lei, Ming-Zhu Xia, Feng-Yun Wang
A Rhodamine-based dual chemosensor L1 for simultaneously detecting Fe(3+) and Cu(2+) was designed and synthesized. The spectroscopic properties of L1 were analyzed, and its recognition mechanism was speculated. We found that the addition of Fe(3+) induced a great fluorescence enhancement, while Cu(2+) induced a strong UV-Vis absorption enhancement. The results revealed that L1 was highly selective for recognizing Fe(3+) and Cu(2+) in UV-Vis spectroscopy in CH3OH-H2O (1/1, v/v, pH 7.2) with the interference of other metal ions...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/27829629/fluidic-culture-and-analysis-of-pulmonary-artery-smooth-muscle-cells-for-the-study-of-pulmonary-hypertension
#9
Kae Sato, Minori Nakajima, Sana Tokuda, Aiko Ogawa
There is an urgent need to develop novel in-vitro models to mimic the disease conditions in pulmonary hypertension (PH). We developed a microfluidic cell culture device for PH studies that withstood high shear stress. Techniques were also developed for cell recovery from the microchannel and mRNA isolation from the collected cells. Using this device, we found that shear stress caused a 7.5-fold increase in the transcription levels of a PH-related molecule, Cyclin D1.
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/27829628/alternation-of-gene-expression-levels-in-mesenchymal-stem-cells-by-applying-positive-dielectrophoresis
#10
Junya Yoshioka, Toru Yoshitomi, Tomoyuki Yasukawa, Keitaro Yoshimoto
In this study, we investigated the effect of positive dielectrophoresis (DEP) on gene expression in mesenchymal stem cells. When applying an alternating current voltage, human bone marrow derived mesenchymal stem cells (UE7T-13) exhibited a positive DEP, and were compressed onto the electrode surface. The constructed device can easily control the DEP force to the cells by changing the frequency. Interestingly, gene expressions of the cell differentiation marker in UE7T-13 cells and the mechanical stimulation-susceptible one were changed by applying a positive DEP...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/27829627/a-perturbation-analysis-to-understand-the-mechanism-how-migrating-cells-sense-and-respond-to-a-topography-in-the-extracellular-environment
#11
Hiromi Miyoshi, Kensuke Suzuki, Jungmyoung Ju, Jong Soo Ko, Taiji Adachi, Yutaka Yamagata
Migrating cells in vivo monitor the physiological state of an organism by integrating the physical as well as chemical cues in the extracellular microenvironment, and alter the migration mode, in order to achieve their unique function. The clarification of the mechanism focusing on the topographical cues is important for basic biological research, and for biomedical engineering specifically to establish the design concept of tissue engineering scaffolds. The aim of this study is to understand how cells sense and respond to the complex topographical cues in vivo by exploring in vitro analyses to complex in vivo situations in order to simplify the issue...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/27829626/design-of-tetra-arm-peg-crosslinked-thermoresponsive-hydrogel-for-3d-cell-culture
#12
Aya Mizutani Akimoto, Erika Hasuike, Hiroto Tada, Kenichi Nagase, Teruo Okano, Hideko Kanazawa, Ryo Yoshida
We synthesized poly(N-isopropylacrylamide) gel containing cell-adhesive peptides, RGDS pendants, and crosslinked by a hydrophilic polymer. Since gelation occurs by simply mixing the polymers, cells can be simultaneously encapsulated inside the gel during gelation. This gel has roughly a 15% volume change between 25 and 37°C, and is transparent at both temperatures. Moreover, adhesion of encapsulated C2C12 cells to the gel could be observed. This thermoresponsive gel would be potentially useful as a cell culture material that can control cell fate by changing mechanical properties of the cell's external environment...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/27829625/combinational-effects-of-polymer-viscoelasticity-and-immobilized-peptides-on-cell-adhesion-to-cell-selective-scaffolds
#13
Rio Kurimoto, Kei Kanie, Koichiro Uto, Shun Kawai, Mitsuo Hara, Shusaku Nagano, Yuji Narita, Hiroyuki Honda, Masanobu Naito, Mitsuhiro Ebara, Ryuji Kato
Immobilization of functional peptides on polymer material is necessary to produce cell-selective scaffolds. However, the expected effects of peptide immobilization differ considerably according to the properties of selected polymers. To understand such combinational effects of peptides and polymers, varieties of scaffolds including a combination of six types of poly(ε-caprolactone-co-D,L-lactide) and four types of cell-selective adhesion peptides were fabricated and compared. On each scaffold, the scaffold properties (i...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/27829624/finite-element-analysis-on-nanomechanical-sensing-of-cellular-forces
#14
Gaku Imamura, Kota Shiba, Genki Yoshikawa
Detection of cellular forces plays an important role in investigating the mechanical basis of cells. As nanomechanical sensors can directly detect surface stress, they can be utilized to detect cellular forces. In the present study, we perform quantitative simulations of nanomechanical sensors for the detection of cellular forces using finite element analyses (FEA). We focus on two types of nanomechanical sensors: a cantilever-type sensor and a membrane-type surface stress sensor (MSS). It is found that sensing signals can be obtained when cells on the nanomechanical sensors synchronize their motions...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/27829623/facile-preparation-of-photoactivatable-surfaces-with-tuned-substrate-adhesiveness
#15
Yoshihisa Shimizu, Masao Kamimura, Shota Yamamoto, Shimaa A Abdellatef, Kazuo Yamaguchi, Jun Nakanishi
This paper describes a facile method for the preparation of photoactivatable substrates with tuned surface density of an extracellular matrix peptide to resolve the impacts of biochemical and mechanical cues on collective cell migration. The controllability of surface ligand density was validated by cell adhesion and migration tests, complemented with fluorescence observation of an alternative ligand. Depending on the surface ligand density, HeLa cells either kept or lost collective characteristics. The present materials will be useful to address mechanobiology of collective cell migration...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/27829622/morphological-and-mechanical-properties-of-osteosarcoma-microenvironment-cells-explored-by-atomic-force-microscopy
#16
Xinlong Wang, Yingjun Yang, Xiaohong Hu, Naoki Kawazoe, Yingnan Yang, Guoping Chen
Cell mechanical properties that depend on cytoskeleton architecture are critical to the mechanotransduction process, and have great potential for cancer diagnosis and therapy. In this study, the morphological and mechanical properties of typical osteosarcoma microenvironment cells, including mesenchymal stem cells (MSC), normal human osteoblast cells (NHOst) and osteosarcoma cells (MG-63), were compared using atomic force microscopy (AFM). The MG-63 cells were smaller and thicker than the MSC and NHOst cells...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/27829621/development-of-microfluidic-systems-for-fabricating-cellular-multilayers
#17
Koji Matsuura, Ikuyo Sugimoto, Yuka Kuroda, Koji Kadowaki, Michiya Matsusaki, Mitsuru Akashi
We designed a microfluidic system comprising microfluidic channels, pumps, and valves to enable the fabrication of cellular multilayers in order to reduce labor inputs for coating extracellular matrices onto adhesive cells (e.g., centrifugation). Our system was used to fabricate nanometer-sized, layer-by-layer films of the extracellular matrices on a monolayer of C2C12 myoblasts. The use of this microfluidic system allowed the fabrication of cellular multilayers in designed microfluidic channels and on commercial culture dishes...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/27829620/a-nanodiamond-peptide-bioconjugate-for-fluorescence-and-odmr-microscopy-of-a-single-actin-filament
#18
Takuya Genjo, Shingo Sotoma, Ryotaro Tanabe, Ryuji Igarashi, Masahiro Shirakawa
Recently, the importance of conformational changes in actin filaments induced by mechanical stimulation of a cell has been increasingly recognized, especially in terms of mechanobiology. Despite its fundamental importance, however, long-term observation of a single actin filament by fluorescent microscopy has been difficult because of the low photostability of traditional fluorescent molecules. This paper reports a novel molecular labeling system for actin filaments using fluorescent nanodiamond (ND) particles harboring nitrogen-vacancy centers; ND has flexible chemical modifiability, extremely high photostability and biocompatibility, and provides a variety of physical information quantitatively via optically detected magnetic resonance (ODMR) measurements...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/27829619/nanoscale-mechanical-stimulation-method-for-quantifying-c-elegans-mechanosensory-behavior-and-memory
#19
Takuma Sugi, Etsuko Okumura, Kaori Kiso, Ryuji Igarashi
Withdrawal escape response of C. elegans to nonlocalized vibration is a useful behavioral paradigm to examine mechanisms underlying mechanosensory behavior and its memory-dependent change. However, there are very few methods for investigating the degree of vibration frequency, amplitude and duration needed to induce behavior and memory. Here, we establish a new system to quantify C. elegans mechanosensory behavior and memory using a piezoelectric sheet speaker. In the system, we can flexibly change the vibration properties at a nanoscale displacement level and quantify behavioral responses under each vibration property...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/27829618/integrin-independent-cell-adhesion-substrates-possibility-of-applications-for-mechanobiology-research
#20
Takashi Hoshiba, Masaru Tanaka
Cells can mainly sense mechanical cues from the extracellular matrix via integrins. Because mechanical cues can strongly influence cellular functions, understanding the roles of integrins in the sensing of mechanical cues is a key for the achievement of tissue engineering. The analyses to determine the roles of integrins in the sensing of mechanical cues have been performed by many methods based on molecular- and cell-biological techniques, atomic force microscopy, and optical tweezers. Integrin-dependent cell adhesion substrates have been also used for this purpose...
2016: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
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