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Letters in Applied Microbiology

F Diánez, M Santos, C Parra, M J Navarro, R Blanco, F J Gea
The antifungal properties of 12 Eos, i.e., Syzygium aromaticum, Pelargonium graveolens, Lavandula angustifolia, Cupresus sempervirens, Mentha piperita, Santolina chamaecyparissus, Citrus sinensis, Pogostemon patchouli, Thymus mastichina, Thymus vulgaris, Eucalyptus globulus and Rosmarinus officinalis. The influence of five doses of each EOs was tested against Botrytis cinerea, Sclerotinia sclerotiorum, Fusarium oxysporum, Phytophthora parasitica, Pythium aphanidermatum, Alternaria brassicae, Cladobotryum mycophilum and Trichoderma aggressivum f...
July 19, 2018: Letters in Applied Microbiology
Soleiman Khedri, Andre Luis Souza Dos Santos, Maryam Roudbary, Ramtin Hadighi, Mehraban Falahati, Shirin Farahyar, Majid Khoshmirsafa, Saeed Kalantari
Oropharyngeal candidiasis is a commonest mucocutaneous infection in HIV-positive individuals. Herein, Samples were taken from oral cavities of 150 HIV-infected patients and cultured on Sabouraud-dextrose agar; 89 (59.3%) of 150 patients had positive culture for Candida and presented clinical sign of classical oral candidiasis. Totally, 102 morphologically distinct colonies were isolated from Candida-positive cultures and subsequently identified by polymerase chain reaction and sequencing assay, presenting the following frequency: 54 C...
July 17, 2018: Letters in Applied Microbiology
Omid Ghafari, Aram Sharifi, Ali Ahmadi, Bahar Nayeri Fasaei
Streptococcus pneumoniae (pneumococcus) is recognized as one of the major cause of infections in communities and hospitals. In this study, anti-pneumococcal and anti-efflux pump activity of two medicinal plants (Thymus daenensis and Origanum vulgare) essential oils were evaluated. Checkerboard assay test was performed for investigation of the effects of selected EOs on ciprofloxacin and ethidium bromide uptake in pmrA overexpressed fluoroquinolone resistant pneumococcus. Using quantitative real-time RT-PCR the PmrA efflux pump gene (pmrA) expression was evaluated following treatment with selected EOs...
July 14, 2018: Letters in Applied Microbiology
Jia Xue, Kevin Caton, Samendra Sherchan
Naegleria fowleri in recreational waters is a serious health threat. A rapid and accurate method to determine this pathogen in water is vital to develop effective control strategies. In this study, we compared two molecular methods: droplet digital polymerase chain reaction (ddPCR) and quantitative PCR (qPCR) assays in identifying N. fowleri from clinical and environmental samples. Strong agreement between ddPCR and qPCR methods over clinical DNA samples was observed. The limit of detection (LOD) for ddPCR and qPCR assays were 2...
July 14, 2018: Letters in Applied Microbiology
Changi Wong, Lee Tung Tan, Aazani Mujahid, Samuel Lihan, Julia Lih Suan Wee, Lik Fong Ting, Moritz Müller
Copper (Cu) tolerance was observed by endophytic fungi isolated from the carnivorous plant Nepenthes ampullaria (collected at an anthropogenically affected site, Kuching city; and a pristine site; Heart of Borneo). The fungal isolates, capable of tolerating copper up to 1,000 ppm (11 isolates in total), were identified through molecular method (ITS4+5; ITS1+NL4; β-tubulin region using Bt2a +Bt2b), and all of them grouped with Diaporthe, Nigrospora, and Xylaria. A Cu-biosorption study was then carried out using live and dead biomass of the 11 fungal isolates...
July 12, 2018: Letters in Applied Microbiology
S L Alves, J M Thevelein, B U Stambuk
In brewing, maltotriose is the least preferred sugar for uptake by Saccharomyces cerevisiae cells. Although the AGT1 permease is required for efficient maltotriose fermentation, we have described a new phenotype in some agt1Δ strains of which the cells do not grow on maltotriose during the first 3-4 days of incubation, but after that, they start to grow on the sugar aerobically. Aiming to characterize this new phenotype, we performed microarray gene expression analysis which indicated upregulation of high-affinity glucose transporters (HXT4, HXT6 and HXT7) and α-glucosidases (MAL12 and IMA5) during this delayed cellular growth...
July 11, 2018: Letters in Applied Microbiology
Z Zhang, X Y Liang, T R Zhang, Q Y Xia, J H Zhang, J L Qu, J N Tang, S D Liu
In the present study, a glutathione S-transferase gene (gst) from sensitive Physarum polycephalum was selected for its ability to detect nanosized TiO2 (nTiO2 ) exposure under dark conditions. The concentration of nTiO2 (25, 40, and 60 nm) for subsequent assays was first determined (5-18 mg mL-1 ) and total GST enzyme activity of P. polycephalum was confirmed to be increased 6-44 fold in groups treated with nTiO2 . Second, an RNA-seq study was performed to indetify candidate gst genes before isolation of an optimum gst gene of P...
July 10, 2018: Letters in Applied Microbiology
Zenat Zebin Hossain, Pimlapas Leekitcharoenphon, Anders Dalsgaard, Rokaia Sultana, Anowara Begum, Peter Kjaer Mackie Jensen, Rene S Hendriksen
Whole Genome Sequencing (WGS) was utilized to investigate the genomic profile of Vibrio cholerae O1 strains, isolated from symptomatic patients in a low-income urban area of Dhaka, Bangladesh. Comparative genomics using bioinformatics tools were applied to identify major virulence factors, biotype and antimicrobial resistance genes in three V. cholerae O1 strains (VC-1, 2 and 3) isolated from two case patients. A phylogenetic SNP (Single Nucleotide Polymorphism) based analysis was conducted to infer the relatedness to V...
July 7, 2018: Letters in Applied Microbiology
Fábio Gonçalves Macêdo de Medeiros, Roberta Targino Pinto Correia, Sébastien Dupont, Laurent Beney, Márcia Regina da Silva Pedrini
Cell osmoporation is a simple and straightforward procedure of creating food-grade bio-capsules. This study proposes a new protocol of sequential cell osmoporation stages and evaluates its impact on the efficiency of curcumin and fisetin internalization into Saccharomyces cerevisiae cells. To the best of our knowledge, this is the first report in the literature regarding the subject. In order to assess how multiple osmoporation stages influence the encapsulation efficiency (% EE), encapsulated amount of curcumin (IC) and fisetin (IF) into S...
July 6, 2018: Letters in Applied Microbiology
Thi Thu Tra Vu, Thomas Alter, Peggy G Braun, Anna J Dittrich, Stephan Huehn
The objective of this study was to determine the effect of HHP on the inactivation of Vibrio spp. in pure cultures and mussel homogenates. Four Vibrio strains including V. alginolyticus, V. cholerae, V. parahaemolyticus and V. vulnificus were used. HHP treatments were performed with both pure Vibrio spp. cultures in alkaline peptone water (2% NaCl) and artificially inoculated mussel homogenates at pressure levels of 250, 350 and 450 MPa for 1 and 3 min at 25°C. Counts of Vibrio were determined before and after treatment using drop plating method...
July 1, 2018: Letters in Applied Microbiology
Ranjit Kour, Sheetal Ambardar, Jyoti Vakhlu
Corm, being a unit of reproduction and storage, plays an indispensable role in the life cycle of Crocus sativus (saffron). Present study deals with the screening of bacteria isolated from cormosphere (area surrounding the corm sheath) of Crocus sativus for biofertilizer and biocontrol property during three growth stages. Out of 1000 producing bacteria isolated from the cormosphere (soil adhering corm sheath in analogy to rhizosphere), 3 PGP Bacilli namely B. thuringiensis DC1, B. megaterium VC3 and B. amyloliquefaciens DC8 were selected based on multiple and maximum PGP traits along with corm rot pathogen antagonism...
June 29, 2018: Letters in Applied Microbiology
J H Liu, L Zhang, D C Zha, L Q Chen, X X Chen, Z M Qi
Microalgae have emerged as promising biosorbents for the treatment of malachite green in wastewater. However, the underlying mechanism for the biosorption of malachite green onto microalgae is still unclear and needs further intensive study. In this work, synchrotron Fourier-transform infrared (synchrotron-FTIR) microspectroscoy in combination with biochemical assay is employed to evaluate malachite green removal efficiency (95.2%, 75.6% and 66.5%) by three stages of Haematococcus pluvialis. Meanwhile, the various vital changes of algal cells including lipids, proteins, polysaccharides and carotenoids, is distinguished and quantified in situ...
June 28, 2018: Letters in Applied Microbiology
Oluwatoyin M Ademola, Taiwo E Adeyemi, Obinna T Ezeokoli, Kolawole I Ayeni, Adewale O Obadina, Yinka M Somorin, Adebukola M Omemu, Rasheed A Adeleke, Cyril C Nwangburuka, Flora Oluwafemi, Olusola B Oyewole, Chibundu N Ezekiel
Analysis of the bacterial community dynamics during the production of traditional fermented condiments is important for food safety assessment, quality control and development of starter culture technology. In this study, bacteria isolated during the processing of iru and ogiri, two commonly consumed condiments in Nigeria, were characterised based on phylogenetic analyses of the bacterial 16S rRNA gene. A total of 227 isolates were obtained and clustered into 12 operational taxonomic units (OTUs) based on 97% 16S rRNA gene similarity...
June 27, 2018: Letters in Applied Microbiology
Supamate Tarasuntisuk, Tanutcha Patipong, Takashi Hibino, Rungaroon Waditee-Sirisattha, Hakuto Kageyama
Mycosporine-2-glycine (M2G), isolated from the halotolerant cyanobacterium Aphanothece halophytica, was purified and characterized in order to determine its utility as a cosmetic and pharmaceutical ingredient. M2G efficiently inhibited protein crosslinking. The inhibitory activity of M2G was significantly greater than that of the well-known Maillard reaction inhibitor Aminogluanide (AG). In addition, M2G, as well as other known mycosporine-like amino acids, inhibited bacterial collagenase activity. To the best of our knowledge, this is the first report describing that M2G specifically inhibits the formation of advanced glycation end-products (AGEs), which play a critical role in aging process and age-related diseases...
June 27, 2018: Letters in Applied Microbiology
Yun Zhang, Yang Zhang, Jie Gao, Qiuxuan Shen, Zhihui Bai, Xuliang Zhuang, Guoqiang Zhuang
Although Nitrobacter winogradskyi is an important chemoorganotrophic organism for the study of nitrite-oxidizing bacteria (NOB) physiology as well as nitrification, until now, the mixotrophic medium for this organism growth has not been optimized, comprehensively. In this study, we aimed to improve the growth medium of N. winogradskyi using the one-factor-at-a-time (NaNO2 , glycerol, pH) method. In addition, a further experimental design was carried out based on Central Composite Design (CCD) with response surface methodology (RSM)...
June 21, 2018: Letters in Applied Microbiology
David Möller, Susanna Kushnir, Marius Grote, Ahmed Ismail-Ali, Kyra R M Koopmans, Fabio Calo, Sascha Heinrich, Bernd Diehl, Frank Schulz
Streptomyces cinnamonensis A495 is a variant of the monensin producer which instead of the native polyether antibiotic gives rise to antibiotic and anti-tumor shunt-product premonensin. Through the supplementation of the fermentation medium with suitable precursors, premonensin can be derivatized via the incorporation of new-to-nature extender units into the biosynthetic machinery. Polyketide extender units require activation, typically in form of coenzyme A thioesters. These are membrane impermeable and thus in the past an artificial mimic was employed...
June 21, 2018: Letters in Applied Microbiology
Y Amagai, Y Makita, M Takai, R Muko, H Matsuda, A Tanaka
Excessive expansion of Staphylococcus aureus is associated with several skin diseases, including atopic dermatitis (AD). Recently, we have demonstrated that washing skins with ultra-pure soft water containing little bivalent metal ions improved skin conditions of atopic subjects. In this study, we investigated the roles of calcium or magnesium on the proliferation of S. aureus both in vitro and in vivo. Depletion of calcium and magnesium in the culture medium significantly suppressed the expansion of S. aureus growth...
June 20, 2018: Letters in Applied Microbiology
C A R Matias, I A Pereira, D P Rodrigues, S Siciliano
This work aimed to investigate the prevalence of Staphylococcus in wild birds seized in illegal trade and their antimicrobial resistance patterns. Cloacal samples were obtained from 109 wild birds apprehended in the street markets in Rio de Janeiro, Brazil. Staphylococcus spp. were pheno and genotypically identified and resistance profile were evaluated by CLSI guidelines and by Polymerase Chain Reaction of mecA and blaZ genes. Staphylococcus was detected in 45,9% (50/109) of the cloacal swab samples and thirty-nine (78,0%) isolates were resistant to one or more of the nine antimicrobials tested, and were also positive to mecA (12/39) or blaZ genes (14/39)...
June 20, 2018: Letters in Applied Microbiology
Misa Otoguro, Shunji Suzuki
Grapevine is one of the most widely grown fruit crops in the world. At present, however, there is much concern regarding chemical pollution in viticulture due to the application of chemical fungicides and fertilizers. One viticultural practice to resolve this issue is the application of microorganisms to grapevine as a substitute for chemicals. Some microorganisms act as an enhancer of grape berry quality as well as a suppresser of disease in grapevine through their antagonistic ability and/or systemic resistance inducing ability...
June 16, 2018: Letters in Applied Microbiology
Laíse Diana Dos Santos Brandão, Douglas Boniek, Maria Aparecida de Resende Stoianoff, Flávia Maria Rezende da Mata, Paulo Roberto Medeiros de Azevedo, José Veríssimo Fernandes, Vânia Sousa Andrade
Vulvovaginal candidiasis (VVC) is an inflammatory disease of the vulva and vagina caused by different yeasts of the genus Candida which is responsible for infection in pregnant patients who attended Maternidade Escola Januário Cicco, Rio Grande do Norte, Brazil. From 41 samples, 19 yeasts were identified phenotypically as Candida albicans, and one as C. glabrata which is reported as the non-albicans species most frequently isolated from vulvovaginitis. The susceptibility to selected antifungal agents (fluocytosine, fluconazole, voriconazole, amphotericin B, caspofungin and micafungin) was determined, and the association between patient related signs and symptoms aided the construction of an epidemiological profile...
June 16, 2018: Letters in Applied Microbiology
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