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Letters in Applied Microbiology

N G Inostroza, P J Barra, L Y Wick, M L Mora, M A Jorquera
Plant-growth promoting rhizobacteria (PGPR) are studied as complements/alternatives to chemical fertilization in agriculture. Poor information however, exists on the potential of PGPR from undisturbed ecosystems. Here, we evaluated the plant growth-promoting (PGP) effect of rhizobacterial consortia from undisturbed Chilean arid-ecosystems (Consortium C1) and agro-ecosystems (Consortium C2) on plant biomass production. The PGP effects of C1 and C2 were assayed in wheat seedlings (Triticum aestivum L.) grown in pots under growth chamber conditions and in pots placed in an open greenhouse under natural conditions, using two different Chilean Andisols (Piedras Negras and Freire series) kept either at 30% or 60% of their maximum water holding capacity (MWHC)...
December 3, 2016: Letters in Applied Microbiology
He Fu, Jun-Jie Zhang, Ying Xu, Hong-Jun Chao, Ning-Yi Zhou
The ortho-nitrophenol (ONP) utilizer Alcaligenes sp. strain NyZ215, meta-nitrophenol (MNP) utilizer Cupriavidus necator JMP134 and para-nitrophenol (PNP) utilizer Pseudomonas sp. strain WBC-3 were assembled as a consortium to degrade three nitrophenol isomers in sequential batch reactors. Pilot test were conducted in flasks to demonstrate that a mixture of three mononitrophenols at 0.5 mM each could be mineralized by this microbial consortium within 84 hours. Interestingly, neither ONP nor MNP was degraded until PNP was almost consumed by strain WBC-3...
November 19, 2016: Letters in Applied Microbiology
Hyoung Jin Kim, Hong-Jin Kim
The development of various types of virus-like particles (VLPs) has accelerated over the past two decades as the importance of VLPs for generating next-generation vaccines has been appreciated. Yeast has advantages such as scalable fermentation, low risk of contamination by adventitious agents, low production costs and the ability to produce VLPs with reliable qualities. It is generally recognized that yeast is suitable for producing VLPs that have simple structures and are produced intracellularly. However, recently there has been much effort to extend its applicability, and there is now evidence that it can be used as an expression platform for the productions of VLPs not only of non-enveloped viruses but also of enveloped viruses...
November 8, 2016: Letters in Applied Microbiology
Norimasa Takasaka, Issara Kaweewan, Mayumi Ohnishi-Kameyama, Shinya Kodani
Based on genome mining, a new antibacterial peptide named actinokineosin was isolated from a rare actinomycete Actinokineospora spheciospongiae. The amino acid sequence of C-terminus of actinokineosin was established by TOF-MS/MS experiment. The amino acid sequence in the macrolactam ring was determined by TOF-MS/MS analyses after cleavage with BNPS-skatole and successive trypsin treatment. As a result of antibacterial assay using paper disk, actinokineosin showed antibacterial activity against Micrococcus luteus at the dosage of 50 μg per disk...
November 4, 2016: Letters in Applied Microbiology
Hiroshi Kinoshita, Sarach Wongsuntornpoj, Fumio Ihara, Takuya Nihira
Rhodotorula species are opportunistic pathogens, which cause not only systemic fungemia but other localized infections. Despite the serious side effects such as nephrotoxicity and hypokalemia, amphotericin B (a polyene antifungal) has been commonly prescribed for Rhodotorula infection because Rhodotorula species are resistant against a candin family of antifungal agents. In this study, novel active compounds against Rhodotorula species were screened from the extracts of entomopathogenic fungi based on the synergistic effect of polyene nystatin (NYS), which causes efficient targeting of compounds due to the increased permeability through the fungal cell membrane...
November 1, 2016: Letters in Applied Microbiology
Dhiviya Prabaa Muthuirulandi Sethuvel, Naveen Kumar Devanga Ragupathi, Shalini Anandan, Balaji Veeraraghavan
Shigellosis represents a major burden of disease in developing countries. A low infectious dose allows the disease to be spread effectively. Although shigellosis is mostly a self-limiting disease, antibiotics are recommended to reduce deaths, disease symptoms, and organism-shedding time. However, In India, antimicrobial resistance among the genus Shigella is more common than among any other enteric bacteria. Notably, new serotypes or subserotypes in Shigella are reported from various parts of the world. Identification of new subserotypes of Shigella spp is becoming a major issue as these strains are non-typeable by conventional serotyping...
October 26, 2016: Letters in Applied Microbiology
V Shapaval, T Møretrø, A Wold Åsli, H P Suso, J Schmitt, D Lillehaug, A Kohler
Microbiological source tracking (MST) for food industry is a rapid growing area of research and technology development. In the paper at hand, a new library-independent approach for microbial source tracking is presented. It is based on a high-throughput liquid micro-cultivation and FTIR spectroscopy. In the approach, FTIR spectra obtained from microorganisms isolated along the production line and a product are compared to each other. We tested and evaluated the new source tracking approach by simulating a source tracking situation...
October 26, 2016: Letters in Applied Microbiology
C Browne, A Loeffler, H R Holt, Y M Chang, D H Lloyd, A Nevel
: Porcine enzootic pneumonia (EP) caused by Mycoplasma hyopneumoniae adversely affects pig welfare and is associated with major economic losses in the pig industry worldwide. Transmission is predominantly by direct contact, but the role of indirect transmission remains poorly understood. This study examined survival of six M. hyopneumoniae isolates dried onto five different surfaces encountered in pig units and exposed to temperatures of 4, 25 and 37°C for up to 12 days. Survival of the organisms was determined by recovering the organism from the surface material and culturing in Friis broth...
October 19, 2016: Letters in Applied Microbiology
Yangyang Ge, Junli Zhu, Xiaofeng Ye
Ten strains were isolated from a spoiled large yellow croaker (Pseudosciaena crocea). All of them were able to grow aerobically from 4 °C to 30 °C, and reduce trimethylamine N-oxide (TMAO) to trimethylamine (TMA) and produce H2 S except the SB01, PF05 and PF07. Biochemical characterization and phylogenetic analysis of 16S rRNA gene showed that eight H2 S producing isolates were closely related to Shewanella baltica, and two isolates PF05 and PF07 were identified as P. fluorescens and P. fragi, respectively...
October 16, 2016: Letters in Applied Microbiology
H W Kim, Y J Hong, J I Jo, S D Ha, S H Kim, H J Lee, M S Rhee
Microbiological quality of 206 raw ready-to-eat seafood samples were investigated according to species (gizzard shad, halibut, rockfish, tuna, oyster, and squid) and distribution channels (fishery, hyper, and online market). Enumeration of Aerobic Plate Count (APC) and total coliforms (TC) and pathogenic bacteria (Bacillus cereus, Staphylococcus aureus, and Vibrio parahaemolyticus) were performed, and level of microbiological quality were classified into four groups: satisfactory, acceptable, unsatisfactory, and unacceptable...
October 16, 2016: Letters in Applied Microbiology
Jian Cheng, Wei Zhuang, Nana Li, Chenglun Tang, Hanjie Ying
Low D-ribose production by B. subtilis was resulted from plenty of acid formation as responsible for carbon overflow. An approach of co-feeding glucose and sodium citrate is developed here and proved to be useful for D-ribose production. This strategy is critical because it affects the cell concentration, the productivity of D-ribose and, especially, the formation of by-products such as acetoin, lactate, and acetate. D-ribose production was increased by 59.6% from 71.06 to 113.41 g l(-1) without acid formation via co-feeding of 2...
October 14, 2016: Letters in Applied Microbiology
B K Pathak, S Mondal, C Barat
: The ability of the ribosome to assist in folding of proteins both in vitro and in vivo is well documented and is a nontranslational function of the ribosome. The interaction of the unfolded protein with the peptidyl transferase centre (PTC) of the bacterial large ribosomal subunit is followed by release of the protein in the folding competent state and rapid dissociation of ribosomal subunits. Our study demonstrates that the PTC-specific antibiotics, chloramphenicol and blasticidin S inhibit unfolded protein-mediated subunit dissociation...
October 14, 2016: Letters in Applied Microbiology
Ja Hyun Lee, Hah Young Yoo, Xiaoguang Yang, Dong Sup Kim, Ju Hun Lee, Soo Kweon Lee, Sung Ok Han, Seung Wook Kim
In our previous study, glycerol was utilized as an additional carbon source for the production of cephalosporin C (CPC) by Acremonium chrysogenum M35. In this study, algal sugars extracted from the third generation biomass, were utilized in the CPC production for the first time. The CPC production improved about two-fold when using the algal sugars as the carbon source. The complex medium including algal sugars and glycerol was utilized, and 7.3 g l(-1) CPC production was achieved in a 250 mL shaking flask...
October 13, 2016: Letters in Applied Microbiology
Jenni Inkinen, Riika Mäkinen, Minna M Keinänen-Toivola, Katrina Nordström, Merja Ahonen
The present study was performed in real life settings in different facilities (hospital, kindergarten, retirement home, office building) with copper and copper alloy touch surface products (floor drain lids, toilet flush buttons, door handles, light switches, closet touch surfaces, corridor hand rails, front door handles, and toilet support rails) parallel to reference products. Pure copper surfaces supported lower total bacterial counts (16 ± 45 vs 105 ± 430 CFU cm(-2) , N = 214, P < 0.001) and lower occurrence of Staphylococcus aureus (2...
October 8, 2016: Letters in Applied Microbiology
Yi Li, Lei Liu, Yanting Xu, Li Ping, Kun Zhang, Xiaobing Jiang, Tianling Zheng, Hailei Wang
Microcystis aeruginosa is a cyanobacterial bloom-causing species and has been considered a serious human health and biological safety threat. In this study, algicidal bacterium h10 showed high algicidal effects on M. aeruginosa 7820, and strain h10 was confirmed to belong to genus Exiguobacterium, for which name Exiguobacterium sp. h10 is proposed. Algicidal activity and mode analysis revealed that supernatant rather than bacterial cells was responsible for showing algicidal activity, which indicated that the algicidal mode of strain h10 is an indirect attack through producing algicidal substances...
October 7, 2016: Letters in Applied Microbiology
Emilia Matallana, Agustín Aranda
Wine yeast deals with many stress conditions during its biotechnological use. Biomass production and its dehydration produce major oxidative stress, while hyperosmotic shock, ethanol toxicity and starvation are relevant during grape juice fermentation. Most stress response mechanisms described in laboratory strains of Saccharomyces cerevisiae are useful for understanding the molecular machinery devoted to deal with harsh conditions during industrial wine yeast uses. However, the particularities of these strains themselves, and the media and conditions employed, need to be specifically looked at when studying protection mechanisms...
October 7, 2016: Letters in Applied Microbiology
Ming-Yi Wang, Jian-Li Geng, Ying-Jian Chen, Yu Song, Mei Sun, Hai-Zhu Liu, Cheng-Jin Hu
Methicillin-resistant staphylococci and ESBL-producing bacteria are the most important resistant pathogens in sepsis. In the study, a new multiplex-touchdown PCR method (MT-PCR) was developed to detect rapidly and simultaneously the presence of mecA, blaSHV , blaCTX-M , blaTEM and blaOXA genes from positive blood culture bottles. The technique showed a sensitivity of 10(3) CFU ml(-1) for mecA detection and of 10(2) CFU ml(-1) for other genes, and 100% specificity in the detection of all genes. All genes were detected in the spiked blood culture bottles artificially contaminated with reference strains...
October 4, 2016: Letters in Applied Microbiology
I Choopara, N Arunrut, W Kiatpathomchai, D Dean, N Somboonna
: We developed an assay comprising crude DNA lysis by simple heat treatment coupled loop-mediated isothermal amplification with hydroxynaphthol blue for Chlamydia trachomatis detection (petty patent pending), and evaluated the developed assay for its feasibility as a one-step point-of-care detection on 284 endocervical swab specimens from clinically symptomatic C. trachomatis and healthy subjects. This assay is sensitive to 0·04 pg of ompA, specific with six primers targeting C. trachomatis ompA region, rapid (45 min total assay time), inexpensive (approx...
September 30, 2016: Letters in Applied Microbiology
Shahram Shafaei, Jörg Dörrstein, Josef Peter Guggenbichler, Cordt Zollfrank
The objective of this research is to develop novel cellulose acetate (biopolymer) composite materials with an excellent antimicrobial activity by embedding molybdenum trioxide particles with unique high specific surface area. High surface area molybdenum trioxide particles were prepared from freshly precipitated molybdenum trioxide dihydrate (MoO3 x2H2 O) and subsequent calcination at 340 °C under H2 /N2 gas. Microbiological evaluation against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were performed applying a roll-on test and excellent antimicrobial activities were determined for composites with embedded anhydrous molybdenum trioxide with a high specific surface area...
September 20, 2016: Letters in Applied Microbiology
Sahreena S Lakhundi, Kwabena O Duedu, Natasha Cain, Reka Nagy, Jakub Krakowiak, Christopher E French
Cellulosic biomass represents a huge reservoir of renewable carbon, but converting it to useful products is challenging. Attempts to transfer cellulose degradation capability to industrially useful microorganisms have met with limited success, possibly due to poorly understood synergy between multiple cellulases. This is best studied by co-expression of many combinations of cellulases and associated proteins. Here we describe the development of a test platform based on Citrobacter freundii, a cellobiose-assimilating organism closely related to Escherichia coli...
September 12, 2016: Letters in Applied Microbiology
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