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Letters in Applied Microbiology

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https://www.readbyqxmd.com/read/29791974/isolation-and-identification-of-vibrio-species-in-the-rio-bravo-grande-and-water-bodies-from-reynosa-tamaulipas
#1
I Guardiola-Avila, V Martínez-Vázquez, R Requena-Castro, K Juárez-Rendón, M G Aguilera-Arreola, G Rivera, V Bocanegra-García
The Rio Bravo (Rio Grande) adjoins various states in the Mexican region and has a great importance in water distribution in the northeast Tamaulipas (Mexico). In this work 161 strains were isolated, identified and characterized from the water samples taken from the flow of the Rio Bravo and the two inner canals that cover Reynosa city. The strains were identified as V. cholerae (74.5%), Vibrio spp. (1.2%) and V. mimicus (0.6%). Furthermore, the detected virulence genes in the V. cholerae strains, were the hlyA, ompU, tcpA, toxR genes in 78...
May 23, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29777636/vntr-analysis-demonstrates-new-patterns-and-high-genetic-diversity-of-leptospira-sp-of-animal-origin-in-brazil
#2
Lauren Hubert Jaeger, Ana Paula Loureiro, Walter Lilenbaum
Leptospirosis is a worldwide zoonosis with a broad host range, including humans and domestic and wild animals. The taxonomic classification of Leptospira species is complex. More recently several molecular tools have been employed in the attempt to group the different strains of leptospires. The objective of the present study was the genetic characterization of Brazilian Leptospira sp. isolates obtained from wild and domestic animals. The genotyping of the strains was performed by the VNTR technique, using the L...
May 19, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29763981/antibacterial-activity-of-cladanthus-arabicus-and-bubonium-imbricatum-essential-oils-alone-and-in-combination-with-conventional-antibiotics-against-enterobacteriaceae-isolates
#3
Abdellah Aghraz, Qada Benameur, Teresa Gervasi, Loubna Ait Dra, Meriem Hind Ben-Mahdi, Mustapha Larhsini, Mohamed Markouk, Nicola Cicero
Multidrug-resistant bacteria have become common all over the world, necessitating the development of new therapeutic strategies. Synergistic interactions between conventional antibiotics and natural bioactive may have therapeutic benefits in a clinical setting. There are plenty of medicinal plants that have proven efficacy against broad spectrum of microorganisms. The aim of the work was to assess the antibacterial activity of Cladanthus arabicus, a Moroccan medicinal plant, and Bubonium imbricatum, a Moroccan endemic plant...
May 15, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29747223/sex-related-differences-in-the-thanatomicrobiome-in-postmortem-heart-samples-using-bacterial-gene-regions-v1-2-and-v4
#4
Courtnee R Bell, Jeremy E Wilkinson, Boakai K Robertson, Gulnaz T Javan
Recent studies have revealed distinct thanatomicrobiome (microbiome of death) signatures in human body sites after death. Thanatomicrobiome studies suggest that microbial succession after death may have the potential to reveal important postmortem biomarkers for the identification of time of death. We surveyed the postmortem microbiomes of cardiac tissues from ten corpses with varying times of death (6-58 h) using amplicon-based sequencing of the 16S rRNA gene' V1-2 and V4 hypervariable regions. The results demonstrated that amplicons had statistically significant (p <0...
May 10, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29740840/antimicrobial-activity-of-an-aspartic-protease-from-salpichroa-origanifolia-fruits
#5
María Eugenia Díaz, Gabriela F Rocha, Francisco Kise, Adriana M Rosso, María Gabriela Guevara, Mónica G Parisi
Plant proteases play a fundamental rol in several processes like growth, development and in response to biotic and abiotic stress. In particular, aspartic proteases are expressed in different plant organs and have antimicrobial activity. Previously, we purified an aspartic protease from Salpichroa origanifolia fruits called salpichroin. The aim of this work was to determine the cytotoxic activity of this enzyme on selected plant and human pathogens. For this purpose, the growth of the selected pathogens was analysed after exposure to different concentrations of salpichroin...
May 8, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29736984/estimation-of-the-bacteriocin-cole7-conjugation-based-kill-anti-kill-antimicrobial-system-by-real-time-pcr-fluorescence-staining-and-bioluminescence-assays
#6
I L Maslennikova, M V Kuznetsova, N Toplak, I V Nekrasova, D Žgur Bertok, M Starčič Erjavec
The efficiency of the bacteriocin, colicin ColE7, bacterial conjugation-based "kill" - "anti-kill" antimicrobial system, was assessed using real-time PCR, flow cytometry and bioluminescence. The ColE7 antimicrobial system consists of the genetically modified Escherichia coli strain Nissle 1917 harbouring a conjugative plasmid (derivative of the F-plasmid) encoding the "kill" gene (ColE7 activity gene) and a chromosomally encoded "anti-kill" gene (ColE7 immunity gene)...
May 7, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29729032/solid-state-fermentation-of-trichoderma-viride-for-enhancement-phenolic-content-antioxidant-and-antimicrobial-activities-in-ginger
#7
Rashad M Saleh, Saleh A Kabli, Saleh M Al-Garni, Maryam A Al-Ghamdi, Azza M Abdel-Aty, Saleh A Mohamed
The phenolic content of methanolic and water extracts of ginger fermented by Trichoderma spp. during solid state fermentation (SSF) was detected as compared with unfermented ginger. The total phenolic content of fermented ginger increased several times. The highest phenolic content of ginger was detected after SSF by T. viride. The optimal physiological conditions for the maximum production of the phenolic content and β-glucosidase activity of fermented ginger by T. viride were detected at day 7 incubation, pH 6...
May 4, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29727485/itraq-based-quantitative-proteomic-analysis-of-differentially-expressed-proteins-in-litopenaeus-vannamei-in-response-to-infection-with-wssv-strains-varying-in-virulence
#8
Xin-Ying Sun, Qing-Hui Liu, Jie Huang
The isobaric tags for relative and absolute quantitation (iTRAQ) was applied to identify differentially expressed proteins (DEPs) in Litopenaeus vannamei in response to different virulence white spot syndrome virus infection. A total of 2,780 unique peptides corresponding to 754 proteins were identified. The number of significant differentially expressed proteins was 161, including 38 up-regulated ones and 123 down-regulated ones in low-virulence infection library compared with normal-virulence infection library...
May 4, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29719930/effect-of-relative-humidity-on-chlorine-dioxide-gas-inactivation-of-foodborne-pathogens-and-its-residues-on-tomatoes
#9
Park Sang-Hyun, Woo-Ju Kim, Dong-Hyun Kang
The effect of relative humidity (RH) on the antimicrobial efficacy of chlorine dioxide (ClO2 ) gas against foodborne pathogens on tomatoes was evaluated. Also, levels of ClO2 residues on tomatoes after exposure to ClO2 gas under different RH conditions were measured to determine the quantity of solubilized ClO2 gas on tomato surfaces. Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes were inoculated onto tomatoes and exposed to ClO2 gas (5, 10, 20, and 30 ppmv) under different RH conditions (50, 70, and 90%)...
May 2, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29709063/evaluation-of-behavior-of-lachancea-thermotolerans-biocontrol-agents-on-grape-fermentations
#10
María Cristina Nally, Lorena María Ponsone, Virginia Mercedes Pesce, María Eugenia Toro, Fabio Vazquez, Sofía Chulze
In previous researches, L. thermotolerans RCKT4 and RCKT5 were showed that inhibited Aspergillus growth. However, currently there are no data about their nutritional preferences, as a possible substrate competitor against S. cerevisiae, and their effects on fermentative process. In the present work we observed that the biocontrol yeasts and S. cerevisiae BSc203, based in the utilization of 16 carbonate sources, reveled significantly differences in the nutritional profile (biocontrol yeasts NS:0.25, BSc203 NS:0...
April 30, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29698572/attenuated-salmonella-vnp20009-mutant-%C3%AE-htra-is-a-promising-candidate-for-bacteria-mediated-tumor-therapy-in-hosts-with-tnfr1-deficiency
#11
Wenling Xu, Taoran Zhou, Junjie Zhou, Zeping Qiang, Jing Zhang, Zi-Chun Hua
VNP20009 is a genetically modified strain of Salmonella typhimurium and has a good anti-cancer effect wildly used in tumor therapy on animal models. For its clinical application, an accurate bio-safety assessment on sensitive models is necessary. In this study, we use TNFR1 KO mice as a susceptive model to assess the virulence of bacterial VNP20009 and its derivative ΔhtrA. By intraperitoneal administration of Salmonella, the increased lethality was observed in TNFR1 KO mice infected with VNP20009, but not with ΔhtrA...
April 26, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29696668/comparative-symbiotic-plasmid-analysis-indicates-that-symbiosis-gene-ancestor-type-affects-plasmid-genetic-evolution
#12
Xinye Wang, Liang Zhao, Lu Zhang, Yuanyuan Wu, Minxia Chou, Gehong Wei
Rhizobial symbiotic plasmids play vital roles in mutualistic symbiosis with legume plants by executing the functions of nodulation and nitrogen fixation. To explore the gene composition and genetic constitution of rhizobial symbiotic plasmids, comparison analyses of 24 rhizobial symbiotic plasmids derived from four rhizobial genera was carried out. Results illustrated that rhizobial symbiotic plasmids had higher proportion of functional genes participating in amino acid transport and metabolism, replication; recombination and repair; carbohydrate transport and metabolism; energy production and conversion and transcription...
April 26, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29679390/clinically-applicable-irreversible-electroporation-for-eradication-of-microorganisms
#13
Maya Korem, Nahum S Goldberg, Amos Cahan, Matan J Cohen, Isaac Nissenbaum, Allon E Moses
Irreversible electroporation (IRE) damages cell membranes and is used in medicine for non-thermal ablation of malignant tumors. Our aim was to evaluate the antimicrobial effect of IRE. The pathogenic microorganisms, S. aureus, S. pyogenes, E. coli, P. aeruginosa and C. albicans were subjected to IRE. Survival was measured as a function of voltage and the number of pulses applied. Combined use of IRE and oxacillin for eradication of S. aureus was also tested. Log10 reduction of microorganisms positively correlated with the number of applied pulses...
April 21, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29679385/genus-level-identification-of-dermatophytes-by-maldi-tof-ms-after-two-days-of-colony-growth
#14
Jari Intra, Cecilia Sarto, Natalia Tiberti, Silvia Besana, Cinzia Savarino, Paolo Brambilla
Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) is becoming a popular technology in clinical microbiology. It is a fast and highly specific method for the routine identification of microorganisms. In this study, we evaluated the suitability of dermatophyte identification after only two days of colony growth using MALDI-TOF MS. Two protein extraction protocols were also evaluated consisting of either formic acid alone or of ethanol-formic acid-acetonitrile to achieve a complete protein extraction...
April 21, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29675838/t4-like-escherichia-coli-phages-from-the-environment-carry-bla-ctx-m
#15
Juliet Roshini Mohan Raj, Rajeshwari Vittal, Prashant Huilgol, Udaya Bhat, Indrani Karunasagar
The resistance determinant blaCTX -M has many variants and has been the most commonly reported gene in clinical isolates of extended spectrum beta-lactamase (ESBL) producing Escherichia coli. Phages have been speculated as potential reservoirs of resistance genes and efficient vehicles for horizontal gene transfer. The objective of the study was to determine the prevalence and characterize bacteriophages that harbour the resistance determinant blaCTX -M . E.coli specific bacteriophages were isolated from 15 samples including soil and water across Mangaluru, India using bacterial hosts that were sensitive to β-lactams...
April 20, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29675942/occurrence-of-plasmid-mediated-quinolone-resistance-genes-in-escherichia-coli-and-klebsiella-spp-recovered-from-corvus-brachyrhynchos-and-corvus-corax-roosting-in-canada
#16
Nicol Janecko, Dana Halova, Ivana Jamborova, Ivo Papousek, Martina Masarikova, Monika Dolejska, Ivan Literak
The spread of antimicrobial resistance from human activity derived sources to natural habitats implicates wildlife as potential vectors of antimicrobial resistance transfer. Wild birds, including corvid species can disseminate mobile genetic resistance determinants through feces. This study aimed to determine the occurrence of plasmid-mediated quinolone resistance (PMQR) genes in Escherichia coli and Klebsiella spp. isolates obtained from winter roosting sites of American crows (Corvus brachyrhynchos) and common ravens (Corvus corax) in Canada...
April 19, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29665083/fda-2014-survey-of-eye-area-cosmetics-for-microbiological-safety
#17
Goran Periz, John Misock, Mei-Chiung Jo Huang, Kapal Dewan, Nakissa Sadrieh
In an attempt to assess cosmetics containing "nontraditional" preservatives, ninety-three eye area cosmetic products were selected based on labeled or marketed claims that these products were "green", "natural", "paraben-free", "preservative-free", or contained nontraditional preservatives (e.g., botanical extracts). Products were analyzed for water activity, pH, and microbiological content, which included enumeration of aerobic microorganisms, detection of microbial growth after a 7-day enrichment, and identification of microbial isolates...
April 17, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29665023/propidium-monoazide-real-time-loop-mediated-isothermal-amplification-for-specific-visualization-of-viable-salmonella-in-food
#18
Jiehong Fang, Yingying Wu, Daofeng Qu, Biao Ma, Xiaoping Yu, Mingzhou Zhang, Jianzhong Han
Traditional DNA-based molecular detection assays, including loop-mediated isothermal amplification (LAMP), are unable to differentiate between viable and dead cells. We developed a rapid and accurate method for detection of viable Salmonella cells in food using calcein dyed visual real-time LAMP combined with propidium monoazide (PMA) treatment. Amplification of DNA from up to 6.3 × 105 CFU ml-1 dead Salmonella cells was inhibited by 5 μmol l-1 PMA dye, whereas the DNA derived from viable cells were detected according the invA gene...
April 17, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29604215/qualitative-and-quantitative-screening-of-the-%C3%AE-glucosidase-activity-in-saccharomyces-cerevisiae-and-saccharomyces-uvarum-strains-isolated-from-refrigerated-must
#19
T Bonciani, L De Vero, E Giannuzzi, A Verspohl, P Giudici
The aim of the present work was to screen a pool of 75 yeasts belonging to the species Saccharomyces cerevisiae and Saccharomyces uvarum in order to select the strains endowed with β-glucosidase activity. The first screening was a qualitative assay based on chromogenic substrates (arbutin and esculin). The second screening was the quantitative evaluation of the β-glucosidase activity via a p-nitrophenyl-β-D-glucopyranoside (p-NPG) assay. The measurement was performed on three different cell preparations, including the extracellular compartment, the cell lysates and the whole cells...
March 31, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29604211/terrestrial-and-marine-antarctic-fungi-extracts-active-against-xanthomonas-citri-subsp-citri
#20
Gabrielle Vieira, Jelena Purić, Luana Galvão Morão, Juliana Aparecida Dos Santos, Fábio José Inforsato, Lara Durães Sette, Henrique Ferreira, Daiane Cristina Sass
This study aims to obtain secondary metabolites extracts from filamentous fungi isolated from soil and marine sediments from Antarctica and assess its potential antibacterial activity on Xanthomonas citri subsp. citri, the agent of citrus canker. Metabolites production was conducted in Malt 2% broth at 15 °C for 20 d after which intracellular and extracellular extracts were obtained. The extracts were evaluated by cell viability assays through Resazurin Microtiter Assay. From 158 fungal extracts, 33 hampered bacterial growth in vitro...
March 31, 2018: Letters in Applied Microbiology
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