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Letters in Applied Microbiology

S Amat, D Baines, T W Alexander
The objectives of this study were to develop a new assay for the evaluation of the antimicrobial activities of essential oils (EOs) in vapor-phase and to demonstrate the antimicrobial activities of commercial EOs against BRPs. To achieve the first objective, a microtube cap containing 100 μl of EO was embedded in an agar plate. An agar plug (diameter 13 mm) inoculated with a bacterial suspension containing10(8) CFU ml(-1) was then placed over the cap and incubated at 37 °C for 24 h. Subsequently, bacteria were recovered from the agar plug by immersion in 5 ml of broth for 10 min, followed by vortexing for 30 s, and the broths were then plated for enumeration...
September 15, 2017: Letters in Applied Microbiology
Ľubomír Valík, Pavel Ačai, Denisa Liptáková
The growth of the oxidatively imperfect yeast Candida maltosa Komagata, Nakase et Katsuya was studied experimentally and modelled mathematically in relation to sodium benzoate and lactic acid concentrations at different temperatures. Application of gamma models for the growth rate resulted in determination of cardinal temperature parameters for the growth environment containing lactic acid or sodium benzoate (Tmin = 0.7/1.3 °C, Tmax = 45.3/45.0 °C, Topt = 36.1/37.0 °C, μopt = 0.88/0.96 h(-1) ) as well as the maximal lactic acid concentration for growth (1...
September 15, 2017: Letters in Applied Microbiology
H Zeitoun, M Bahey-El-Din, M A Kassem, H M Aboushleib
Mycobacterium tuberculosis infection constitutes a global threat that results in significant morbidity and mortality worldwide. Efficient and early diagnosis of tuberculosis (TB) is of paramount importance for successful treatment. The aim of the current study is to investigate the mycobacterial mycothiol acetyltransferase Rv0819 as a potential novel biomarker for diagnosis of active TB infection. The gene encoding Rv0819 was cloned and successfully expressed in Escherichia coli. The recombinant Rv0819 was purified using metal affinity chromatography and was used to raise murine polyclonal antibodies against Rv0819...
September 14, 2017: Letters in Applied Microbiology
P Rameshkumar, A K A Nazar, M A Pradeep, C Kalidas, R Jayakumar, G Tamilmani, M Sakthivel, A K Samal, S Sirajudeen, V Venkatesan, B M Nazeera
Mass mortalities of cobia Rachycentron canadum sub-adults occurred during August 2013 in cage culture at the Gulf of Mannar, Mandapam Tamil Nadu, India. The epizootic of disease was started with typical classical clinical signs followed by the acute mortality. Grossly, severe haemorrhage and congestion was observed in the gastric mucosa. Abdomen was distended with peritoneal fluid. Heart revealed haemopericardium and fibrinous pericardium. Histologically, the gastric mucosa showed severe erosion and necrosis...
September 12, 2017: Letters in Applied Microbiology
Natalie Naidoo, Meenu Ghai, Kiresen Moodley, Lwamkilekele Mkhize, Lauren Martin, Sharon McFarlane, Stuart Rutherford
Ratoon stunt caused by bacterium Leifsonia xyli subsp. xyli (Lxx) results in substantial yield losses in sugarcane (Saccharum spp. L. hybrid). Since RS does not produce reliable symptoms in the field, laboratory-based techniques are necessary for detection. Loop-Mediated Isothermal Amplification (LAMP) assay overcomes the limitations of laboratory-based techniques which are costly, time consuming and cannot be used for near-field detection. A sensitive LAMP assay was developed to detect Lxx at 65°C in 30 min...
September 6, 2017: Letters in Applied Microbiology
Wilson José Fernandes Lemos Junior, Alessia Viel, Barbara Bovo, Milena Carlot, Alessio Giacomini, Viviana Corich
AIMS: In this work fermentation performances of seven vineyard strains, together with the industrial strain EC1118, have been investigated at three differing yeast assimilable nitrogen concentrations (300 mg N l(-1) , 150 mg N l(-1) and 70 mg N l(-1) ) in synthetic musts. METHODS AND RESULTS: Most of the strains showed a dramatic decrease of the fermentation at 70 mg N l(-1) but no significant differences in CO2 production were found when fermentations at 300 mg N l(-1) and 150 mg N l(-1) were compared...
September 1, 2017: Letters in Applied Microbiology
K Maeda, M Izawa, Y Nakajima, Q Jin, T Hirose, T Nakamura, H Koshino, K Kanamaru, S Ohsato, T Kamakura, T Kobayashi, M Yoshida, M Kimura
Histone deacetylases (HDACs) play an important role in the regulation of chromatin structure and gene expression. We found that dark pigmentation of Magnaporthe oryzae (anamorph Pyricularia oryzae) ΔMohda1, a mutant strain in which an ortholog of the yeast HDA1 was disrupted by double cross-over homologous recombination, was significantly stimulated in liquid culture. Analysis of metabolites in a ΔMohda1 mutant culture revealed that the accumulation of shunt products of the 1,8-dihydroxynaphthalene melanin and ergosterol pathways were significantly enhanced compared to the wild-type strain...
September 1, 2017: Letters in Applied Microbiology
Mehrdad Gholami, Alireza Salimi Chirani, Shabnam Razavi, Reza Falak, Gholamreza Irajian
Interference with bacterial adhesion is a new means to prevent or treat bacterial infections. In this experimental study we evaluated the immunogenic properties of a chimeric protein composed of PilQ and disulfide turn region of PilA from Pseudomonas aeruginosa in mice as an anti-adhesion based vaccine. First of all, a chimeric bivalent protein composed of PilQ and PilA was constructed and following subcutaneous immunization with merely the purified protein or in its admixed form with alum, the immunogenicity of the chimeric antigen was assessed in BALB/c mice...
August 31, 2017: Letters in Applied Microbiology
Caili Zhang, Suqin Zhu, Abdul-Nabi Jatt, Yurong Pan, Mingyong Zeng
Shewanella spp. are the common spoilage organisms found in aquatic food products stored at low temperature and their spoilage mechanism has been reported to be mediated by quorum sensing (QS). However, the specifically expressed proteins responding to N-acyl homoserine-lactone (AHLs) were seldom reported. The present study aims to evaluate the effects of different AHL signal molecules on Shewanella putrefaciens Z4 isolated from refrigerated turbot (Scophthalmus maximus) at the proteome level. The results revealed that exogenous AHLs were utilized as QS signal molecules by S...
August 21, 2017: Letters in Applied Microbiology
Douglas Boniek, Isolda de Castro Mendes, Christiane Abreu Oliveira Paiva, Ubiraci Gomes de Paula Lana, Antônio Fernando Batista Dos Santos, Maria Aparecida de Resende Stoianoff
The present study aimed to evaluate the action of organic acids produced by the fungal population associated with the biodeterioration process of the Twelve Prophets of Aleijadinho, a set of soapstone sculptures in Congonhas, state of Minas Gerais, Brazil. For this, samples of fungi were obtained from the surface of each of the twelve outdoor stone sculptures that comprise the set of prophets. Identification of the colonizing filamentous fungi was performed by classical microbiology and molecular methods. Some species of filamentous fungi-dependent cultivation were detected, and the presence of species Aspergillus versicolor, Curvularia lunata, Epicoccum nigrum, Penicillium citrinum, and Pseudocercospora norchiensis indicated a connection with the excretion of organic acids...
August 20, 2017: Letters in Applied Microbiology
Anwesa Bag, Rabi Ranjan Chattopadhyay
The aim of the study was to evaluate possible antibacterial and antibiofilm efficacy of a bacteriocin, nisin with two essential oil components linalool and p-coumaric acid in combination against foodborne bacteria Bacillus cereus and Salmonella typhimurium. Their inhibition effects on planktonic cells and pre-formed biofilms were evaluated using microbroth dilution and checkerboard titration methods. Nisin/ p-coumaric acid combination showed synergistic effects against planktonic cells of both the studied bacteria whereas nisin/ linalool combination showed synergistic activity against B...
August 16, 2017: Letters in Applied Microbiology
Louis D Saravolatz, Joan Pawlak, Hayley Martin, Stephanie Saravolatz, Leonard Johnson, Hedda Wold, Mette Husbyn, Wenche Marie Olsen
The development of new synthetic antimicrobial peptides like LTX-109 provides a new class of drugs for the treatment of Staphylococcus aureus infections. We evaluated LTX-109 and mupirocin for pharmacodynamic parameters against 10 methicillin-resistant S. aureus isolates. The post antibiotic effect (PAE) is defined as the length of time that bacterial growth is suppressed following a brief exposure to an antibiotic. We also determined the sub-MIC effect (SME) which measures the direct effect of sub-inhibitory levels on strains that have not previously been exposed to antibiotics...
August 12, 2017: Letters in Applied Microbiology
Tareq M Osaili, Anas A Al-Nabulsi, Dima S Nazzal, Reyad R Shaker
The presence of Salmonella spp. in halva has been associated with foodborne illnesses and product recalls from the markets. This study investigated the effect of environmental stresses on the survival of Salmonella spp. in halva during storage for 12 months at 10 and 25°C. Halva samples were inoculated with a cocktail of four strains of unstressed, desiccation stressed or heat stressed Salmonella (10(6) -10(7) CFU g(-1) ). In general, survival of Salmonella spp. in halva decreased significantly (P ˂ 0.05) as storage time and temperature increased...
August 12, 2017: Letters in Applied Microbiology
T Pimchan, D Maensiri, G Eumkeb
To address the resistance of Acinetobacter baumannii to β-lactam antibiotics, combination therapy between different antibiotic classes is increasingly used. The antibacterial activity of α-mangostin (AMT) alone or in combination with ceftazidime (CTZ) was investigated against ceftazidime-resistant A. baumannii DMST 45378 (CRAB). Initial screening showed that A. baumannii strains possessed AmpC β-lactamase (AmpC), extended-spectrum beta-lactamase (ESBL) and metallo-β-lactamases (MBL). Minimum inhibitory concentrations (MICs) of all test agents were >800 μg ml(-1) against CRAB...
August 11, 2017: Letters in Applied Microbiology
Yang Wang, Honghai Zhang, Chen Zhao, Yitao Han, Yuchun Liu, Xiaolin Zhang
A strain DDB001 having an ability to remove DON was successfully isolated from wheat field soil. On the basis of phenotypic, genotypic, and phylogenetic data, the strain DDB001 was identified as Paradevosia shaoguanensis. Strain DDB001 could grow well and thoroughly eliminate 200 mg l(-1) of DON in complete growth medium, but it could not utilise DON as the sole carbon source for growth in mineral salt medium. Analysing DON transformation products by HPLC-MS assumed that the strain could transform DON into a less toxic stereoisomer, 3-epi-deoxynivalenol (3-epi-DON)...
August 11, 2017: Letters in Applied Microbiology
Leticia da Silva Portes, Erika Seki Kioshima, Zoilo Pires de Camargo, Wagner Luiz Batista, Patricia Xander
Paracoccidioidomycosis (PCM) is a systemic granulomatous disease endemic in Latin America whose aetiologic agents are the thermodimorphic fungi Paracoccidioides brasiliensis and Paracoccidioides lutzii. Despite technological advances, some problems have been reported for the fungal antigens used for serological diagnosis, and inconsistencies among laboratories have been reported. The use of synthetic peptides in the serological diagnosis of infectious diseases has proved to be a valuable strategy because in some cases, the reactions are more specific and sensitive...
August 10, 2017: Letters in Applied Microbiology
Sunil Sethi, Rakesh Yadav, Shreya Singh, Rajiv Khaneja, Ashutosh Aggarwal, Priyanka Agarwal, Digambar Behera
Multidrug resistant tuberculosis (MDR-TB) is rising and World health organization has recommended the line probe assay (LPA) for screening. In this study we assess LPA at a tertiary care center from North India in 1758 samples from suspected MDR-TB cases. All smear positive and/or M.tuberculosis culture confirmed cases (n=1170) were subjected to GenoType-MTBDR assay. Amongst these the majority were retreatment cases, smear positive at diagnosis (n=637). MDR prevalence of 7.8% was observed with the highest cases reported amongst MDR contacts (33...
August 9, 2017: Letters in Applied Microbiology
H Cai, T Zhang, M Zhao, J Mao, C Cai, F Feng
To enhance the overall expression level of lipase isozymes which catalyse the same reaction in Pichia pastoris through co-expression of isozymes from different sources; several types of co-expression ways were constructed to determine the co-expression efficiencies of lipase isozymes in P. pastoris. The results showed that the Kex2-mediated co-expression of lipase isozymes could express Rhizomucor miehei lipase (RML) and Thermomyces lanuginosus lipase (TLL) simultaneously, and GS-RMk-kTL displayed an average lipase activity of 306·91 U ml(-1) , higher than GS-RML and GS-kTL (2·89 and 300·59 U ml(-1) ) expressed independently in P...
August 1, 2017: Letters in Applied Microbiology
A Tarafdar, A Sinha, R E Masto
The current study is aimed to evaluate the mechanism of anthracene degradation by a bacterial strain isolated from fly ash deposition site near Jamadoba Coal Preparation Plant, Jharkhand, India. The Bushnell-Haas media cultured (containing anthracene as sole carbon source) bacterial isolate was identified by 16S rRNA gene sequence coding as the Bacillus thuringiensis strain, which showed the efficiency to degrade anthracene. The degradation efficiency of the strain has been estimated to be around 91% (for 40 mg l(-1) of anthracene concentration) after 2 weeks of incubation at 33-36°C and initial pH of 6·8-7...
August 1, 2017: Letters in Applied Microbiology
Yuhan Liu, Yinbin Xu, Shuang Li, Xuechuan Xu, Qi Gao, Meijun Yuan, Wei Gu, Wen Wang, Qingguo Meng
Spiroplasma eriocheiris, which causes tremor disease of Chinese mitten crab Eriocheir sinensis, has led to catastrophic economic loses in aquaculture. Immunoproteomics, a new scientific technique combining proteomics and immunological analytical methods, provided the direction of our research on S. eriocheiris. The aim of our study was to identify the proteome, antigen proteins and antigen membrane proteins of S. eriocheiris. A total of 780 S. eriocheiris proteins were identified by LC-MS/MS technique. Based on immunoproteomics, 51 proteins and 7 proteins in S...
August 1, 2017: Letters in Applied Microbiology
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