Read by QxMD icon Read

Letters in Applied Microbiology

James Redfern, Joanna Verran
Listeria monocytogenes is a pathogenic bacterium, with human disease and infection linked to dairy products, seafood, ready-to-eat meat and raw & undercooked meats. Stainless steel is the most common food preparation surface and therefore, it is important to understand how food storage conditions such as surface materials, temperature and relative humidity can affect survival of L. monocytogenes. In this study, survival of L. monocytogenes on stainless steel was investigated at three temperatures (4, 10 and 21°C), each approx...
January 19, 2017: Letters in Applied Microbiology
Yi Yang, Song Huang, Juan Wang, Gwénaël Jan, Romain Jeantet, Xiao Dong Chen
Food related carbohydrates and proteins are often used as thermoprotectant for probiotic lactobacilli during industrial production and processing. However, the effect of inorganic salts is rarely reported. Magnesium is the second-most abundant cation in bacteria, and commonly found in various foods. Mg(2+) homeostasis is important in Salmonella and has been reported to play a critical role in their thermotolerance. However, Mg(2+) role in thermotolerance of other bacteria, in particular probiotic bacteria, still remains a hypothesis...
January 18, 2017: Letters in Applied Microbiology
Mansoor Kodori, Masoumeh Douraghi, Mehdi Yaseri, Mohammad Rahbar
The Acinetobacter baumannii virulence protein Bap is encoded by a large gene and contains both variable sequence and repetitive modules. To date, four primer sets targeting different regions of bap have been designed, but no study has evaluated all these primers simultaneously for detection of bap. Here, we assessed the effect of primer sets Bap I to IV, on detection of bap both in silico and in vitro. Using the primer set Bap II, all 143 tested strains yielded an amplicon corresponding to bap gene. This primer set showed the highest sensitivity (100%, 95% CI: 97...
January 18, 2017: Letters in Applied Microbiology
Chenfei Zhang, Zhangong Yang, Wen Jin, Xiang Wang, Yingkun Zhang, Shijun Zhu, Xing Yu, Gang Hu, Qing Hong
Methomyl [S-methyl N-(methylcarbamoyloxy) thioacetimidate] is a kind of oxime carbamate insecticide. It is considered to be extremely toxic to non-target organism. Up to date, no pure culture or consortium has been reported to have the ability to degrade methomyl completely. In the present study, a methomyl-degrading enrichment E1 was obtained by using the sludge from the wastewater-treating system of a pesticide manufacturer as the original inoculant. Two bacterial strains named MDW-2 and MDW-3 were isolated from this enrichment, and they were preliminarily identified as Aminobacter sp...
January 13, 2017: Letters in Applied Microbiology
Amoolya Narayanan, Muhammed Shafeekh Muyyarikkandy, Shankumar Mooyottu, Kumar Venkitanarayanan, Mary Anne Roshni Amalaradjou
Urinary tract infections (UTIs) in the United States result in more than 7 million hospital visits per year. Uropathogenic E. coli (UPEC) is responsible for more than 80% of UTIs. Although antibiotics are the drug of choice to control UTIs, their repeated use has resulted in the emergence of antibiotic resistant UPEC. Thus, there is a need for effective alternate strategies to control UPEC infections. This study investigated the efficacy of trans-cinnamaldehye (TC), a food-grade molecule present in cinnamon, in reducing UPEC colonization and pathogenesis in the lower UTI...
January 7, 2017: Letters in Applied Microbiology
Lina Mendez-Castillo, Erlide Prieto-Correa, Carlos Jimenez-Junca
Filamentous fungi are an unexplored source for the production of biosurfactants, but over a decade it was discovered one of the most surface active molecules called hydrophobins. There are few techniques to determine the surface activity of fungi without some kind of manipulation that can affect the final results. In this work, we identified 33 strains of filamentous fungi isolated from banana rachis which may have potential in producing biosurfactants. Further the production of surface active compounds by the strains was measured by two techniques...
January 6, 2017: Letters in Applied Microbiology
Xiaowen He, Yinzheng Ma, Guohui Yi, Jinyan Wu, Limin Zhou, Hong Guo
In recent years, the incidence of clinical yeast infections has increased dramatically. Due to the extensive use of broad-spectrum antifungal agents, there has been a notable increase in drug resistance among infections yeast species. As one of the most popular natural antimicrobial agents, essential oils (EOs) have attracted a lot of attention from the scientific community. The aim of this study was to analyze the chemical composition and examine the antifungal activity of the EO extracted from the seeds of Carica papaya Linn...
January 4, 2017: Letters in Applied Microbiology
Andrew Fox, Bruno Pichon, Hayley Wilkinson, Michel Doumith, Robert L R Hill, Jim McLauchlin, Angela M Kearns
Limited data are available on the prevalence of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) in the UK. We tested 124 raw meat samples for MRSA including pork (n=63), chicken (n=50) and turkey (n=11) collected from retail outlets in North West England between March and July 2015. MRSA was recovered from 9 (7.3%) samples (4 chicken, 3 pork, 2 turkey) from different butchers and supermarkets. Four were labelled of UK origin, 3 were from continental Europe; the origin was not specified for 2...
December 30, 2016: Letters in Applied Microbiology
P D Rekha, H S Vasavi, C Vipin, K Saptami, A B Arun
Quorum sensing (QS) has been shown to play a crucial role in the pathogenesis in many bacteria and attenuation of QS is one of the targets of antimicrobial therapy with particular interest in combating drug resistance. This study reports the QS inhibitory activity of metabolites from Cassia alata L. (Ca. alata), an important medicinal herb widely used in the treatment of microbial infections. For investigating the QS inhibition (QSI) potential of Ca. alata L., initially, metabolites of the leaves extracted using ethanol were tested against biosensor strain Chromobacterium violaceum CV026 and C...
December 30, 2016: Letters in Applied Microbiology
Syed Imteyaz Alam, Annu Uppal, Pallavi Gupta, Dev Vrat Kamboj
Clostridium perfringens epsilon toxin (ETX), staphylococcal enterotoxin B (SEB), and shiga toxin (STX) are implicated in a number of diseases and food-borne intoxications and are considered as potential agents for bioterrorism and warfare. Artificially generated aerosol is the likely mode of delivery of these toxins for nefarious uses; potentially capable of causing mass destruction to human and animal health by inhalation of toxic bioaerosol. Multiplex and unambiguous detection of these agents is of paramount importance for emergency response in a biothreat scenario and for food safety...
December 26, 2016: Letters in Applied Microbiology
K L Hillitt, R E Jenkins, O B Spiller, M L Beeton
The susceptibility of the cell-wall free bacterial pathogens Ureaplasma spp. to Manuka honey was examined. The minimum inhibitory concentration (MIC) of Manuka honey for four Ureaplasma urealyticum and four Ureaplasma parvum isolates was determined. Sensitivity to honey was also compared to clinical isolates with resistance to tetracycline, macrolide and fluoroquinolone antibiotics. Finally step-wise resistance training was utilised in an attempt to induce increased tolerance to honey. The MIC was dependent on the initial bacterial load with 7...
December 19, 2016: Letters in Applied Microbiology
Kien-Cuong Pham, Hoa Thi Tuyet Tran, Cuong Van Doan, Phuoc Hong Le, Anh Thi Van Nguyen, Hoa Anh Nguyen, Huynh Anh Hong, Simon M Cutting, Tuan-Nghia Phan
In this study, Bacillus subtilis spores expressing a chimeric protein, CotB-VP28, was used as a probiotic vaccine to protect black tiger shrimps (Penaeus monodon) against White Spot Syndrome virus (WSSV) infection. Oral administration of pellets coated with CotB-VP28 spores (at >1 x 10(9) cfu g(-1) pellet) to shrimps induced immune-relating phenoloxydase activity (PO) in shrimps after 14 days of feeding (prior challenge) and at day 3 post challenge (1.26 and 1.70 fold increase, respectively). A 75% protection rate was obtained by continuous feeding of the spore coated pellets at >1 x 10(9) cfu g(-1) for 14 days prior to WSSV challenge and during all the post-challenge period...
December 19, 2016: Letters in Applied Microbiology
M M Suyemoto, Hjb Barnes, L B Borst
Pathogenic strains of Enterococcus cecorum (EC) expressing multidrug resistance have emerged. In National Antimicrobial Resistance Monitoring System (NARMS) data, EC is rarely recovered from chickens. Two NARMS methodologies (FDA, USDA) were compared with standard culture (SC) techniques for recovery of EC. NARMS methods failed to detect EC in 58 cecal samples, 20 chicken breast or 6 whole broiler samples. EC was recovered from 1 of 38 (2.6%) and 2 of 38 (5.2%) pre-harvest spinal lesions (USDA and FDA method respectively)...
December 17, 2016: Letters in Applied Microbiology
Rachael Reid, Séamus Fanning, Paul Whyte, Joe Kerry, Declan Bolton
The aim of this study was to investigate if rapid slurry chilling would retard or prevent blown pack spoilage (BPS) of vacuum packaged beef primals. Beef primals were inoculated with Clostridium estertheticum subspp. estertheticum (DSMZ 8809), C. estertheticum subspp. laramenise (DSMZ 14864) and C. gasigenes (DSMZ 12272) and vacuum packaged with and without heat shrinkage (90°C for 3 seconds). These packs were then subject to immediate chilling in an ice slurry or using conventional blast chilling systems and stored at 2°C for up to 100 days...
December 16, 2016: Letters in Applied Microbiology
Sandra Wegner, Julia I Bauer, Richard Dietrich, Erwin Märtlbauer, Ewald Usleber, Christoph Gottschalk, Madeleine Gross
A simplified method to produce specific polyclonal rabbit antibodies against sterigmatocystin (STC) was established, using a STC-glycolic acid-ether derivative (STC-GE) conjugated to keyhole limpet hemocyanin (immunogen). The competitive direct enzyme immunoassay (EIA) established for STC had a detection limit (20% binding inhibition) of 130 pg ml(-1) . The test was highly specific for STC, with minor cross-reactivity with O-methylsterigmatocystin (OMSTC, 0.87%) and negligible reactivity with the aflatoxins (<0...
December 16, 2016: Letters in Applied Microbiology
A Shirai, T Watanabe, H Matsuki
The low energy of UV-A (315-400 nm) is insufficient for disinfection. To improve UV-A disinfection technology, the effect of ferulic acid (FA) addition on inactivation by UV-A light-emitting diode (LED) light (350-385 nm) was evaluated in the eliminating of suspensions of various food spoilers and pathogens (seven bacteria and four fungi). Photoantimicrobial assays were performed at FA concentrations below the MIC. The MIC of the isomerized FA, consisting of 93% cis-form and 7% trans-form, was very similar to that of the commercially available FA (trans-form)...
December 16, 2016: Letters in Applied Microbiology
J L Lin, Y Peng, Q T Ou, D L Lin, Y Li, X H Ye, J L Zhou, Z J Yao
Methicillin-resistant Staphylococcus aureus (MRSA) has caused a series of public health problems since it was first found in 1961. However, there are few research studies on the MRSA environmental contamination in railway stations and coach stations. Therefore, the aim of this study was to determine MRSA environmental contamination in public transport stations. Between December 2013 and January 2014, 380 surface samples from three railway stations (180) and four coach stations (200) in Guangzhou were collected to isolate and determine the prevalence and characteristics of Staphylococci strains...
December 8, 2016: Letters in Applied Microbiology
Werner Rossouw, Lise Korsten
Microbial dynamics on commercially grown white button mushrooms is of importance in terms of food safety assurance and quality control. The purpose of this study was to establish the microbial profile of fresh white button mushrooms. The total microbial load was determined through standard viable counts. Presence and isolation of gram-negative bacteria including coagulase positive Staphylococci were performed using a selective enrichment approach. Dominant and presumptive organisms were confirmed using molecular methods...
December 8, 2016: Letters in Applied Microbiology
Yenizey M Alvarez-Cisneros, Francisco J Fernández, Teresita Del Rosario Sáinz Espuñes, Edith Ponce-Alquicira
Enterococcus faecium MXVK29 has the ability to produce an antimicrobial compound that belongs to Class IIa of the Klaenhammer classification, and could be used as part of a biopreservation technology through direct inoculation of the strain as a starter or protective culture. However, Enterococcus is considered an opportunistic pathogen, so the purpose of this work was to study the food safety determinants of Enterococcus faecium MXVK29. The strain was sensitive to all of the antibiotics tested (penicillin, tetracycline, vancomycin, erythromycin, chloramphenicol, gentamicin, neomycin, kanamycin and netilmicin) and did not demonstrate histamine, cadaverine, or putrescine formation...
December 8, 2016: Letters in Applied Microbiology
N G Inostroza, P J Barra, L Y Wick, M L Mora, M A Jorquera
Plant-growth promoting rhizobacteria (PGPR) are studied as complements/alternatives to chemical fertilization in agriculture. Poor information however, exists on the potential of PGPR from undisturbed ecosystems. Here, we evaluated the plant growth-promoting (PGP) effect of rhizobacterial consortia from undisturbed Chilean arid-ecosystems (Consortium C1) and agro-ecosystems (Consortium C2) on plant biomass production. The PGP effects of C1 and C2 were assayed in wheat seedlings (Triticum aestivum L.) grown in pots under growth chamber conditions and in pots placed in an open greenhouse under natural conditions, using two different Chilean Andisols (Piedras Negras and Freire series) kept either at 30% or 60% of their maximum water holding capacity (MWHC)...
December 3, 2016: Letters in Applied Microbiology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"