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Letters in Applied Microbiology

Sabrina Hossain, B C J De Silva, P S Dahanayake, Gang-Joon Heo
Aeromonas spp. are opportunistic pathogenic bacteria associated with a multitude of diseases in ornamental fish. In this study, virulence properties and antibiotic resistance patterns of 43 Aeromonas strains isolated from 46 zebrafish were investigated. The isolates were identified as A. veronii biovar veronii (n=26), A. veronii biovar sobria (n=3), A. hydrophila (n=8), A. caviae (n=3), A. enteropelogenes (n=2) and A. dhakensis (n=1) by gyrB gene sequencing. The sequence divergence within and between the species ranged from 0-5...
September 19, 2018: Letters in Applied Microbiology
Analía Montemarani, Melina Sartori, Andrea Nesci, Miriam Etcheverry, Germán Barros
Northern corn leaf blight (NCLB) caused by Exserohilum turcicum is an endemic foliar disease in the Argentinean maize production area. Before applying a control method, it is essential to focus on factors that lead to the survival and conservation of E. turcicum inoculum. However, there is little information about the life cycle of this emerging pathogen in our country. The objective of the present work was to analyze the growth ability of three E. turcicum isolates under different type and conditions of maize, wheat and soybean residues, matric potential, temperature and their interactions...
September 19, 2018: Letters in Applied Microbiology
V S Cheptsov, E S Churbanova, V I Yusupov, M V Gorlenko, L V Lysak, N V Minaev, V N Bagratashvili, B N Chichkov
Recently it was shown that laser-induced forward transfer (LIFT) technology and the laser engineering of microbial systems (LEMS) technique (based on LIFT method) are effective for isolation of microorganisms from different complex substrates. These techniques frequently utilize Au as an absorbing layer material. The purpose of present study was to investigate the influence of absorbing film materials (Au, Ti, and Cr) on the effectiveness of laser printing of microorganisms to improve LEMS and LIFT techniques...
September 17, 2018: Letters in Applied Microbiology
M F P Domingos-Lopes, P Lamosa, C Stanton, R P Ross, C C G Silva
High molar mass exopolysaccharides (EPS) produced from sucrose by lactic acid bacteria (LAB) are of great interest as natural additives to use in foods, medical and pharmaceutical industry. This study aimed to identify the EPS produced by Leuconostoc citreum L3C1E7 isolated from Pico cheese and characterize the strain for technological and probiotic potential. Purified EPS was isolated from the culture of Leuconostoc citreum L3C1E7 by ethanol precipitation, with a yield of 520 mg ml-1 . The EPS producing-strain had a mucoid phenotype and average molecular weight of 5...
September 15, 2018: Letters in Applied Microbiology
Kristie Keeney, Aljosa Trmcic, Zheqing Zhu, Pascal Delaquis, Siyun Wang
Listeria monocytogenes has a significant impact on the food industry by forming biofilms on food-processing equipment. Tandem analysis of whole-genome sequencing data with biofilm data from 166 environmental and food-related L. monocytogenes isolates has revealed serotypic and genetic factors that strongly correlate with adherence and biofilm formation, such as lineage, plasmid harborage, a three codon-deletion in inlA and the presence of the Stress Survival Islet 1 (SSI-1). Strains from serotype 1/2b, the majority of which contained SSI-1, formed the strongest biofilms, while serotype 4b strains, the majority of which did not contain SSI-1, formed the weakest biofilms...
September 15, 2018: Letters in Applied Microbiology
Govindan Krishnamoorthy, Suganya Kannan, Murugan Marudhamuthu
The present study focused on the evaluation of fungal compound for their anti-pathogenic potential against respiratory pathogens. Soil samples were collected from various geographical regions in Madurai, fungal strain was isolated and identified as Aspergillus terreus DMTMGK004 (MGK004). Secondary metabolites were extracted and evaluated for antioxidant potential. It exhibited significantly high anti-proliferative property against gastric adeno carcinoma (AGS) cell lines. Antimicrobial activity against Gram positive (Streptococcus pneumoniae) and Gram negative (Klebsiella pneumoniae and Haemophilus influenzae) respiratory pathogens were analyzed and the minimum inhibitory concentration (MIC) values were determined...
September 11, 2018: Letters in Applied Microbiology
Qiaopeng Tian, Yuanyuan Feng, Hao Huang, Junxuan Zhang, Yihang Yu, Zhengbing Guan, Yujie Cai, Xiangru Liao
The aim of this study was to produce lactobionic acid from lactose by a new Pycnoporus sp. SYBC-L10 strain. Recently studies on enzymatic production of lactobionic acid mostly focus on cellobiose dehydrogenase from S. rolfsii CBS 191.62 and laccase from T. pubescens MB 89 oxidize lactose to lactobionic acid with redox mediators. In this study, we converted lactose to lactobionic acid by shaking flask fermentation without exogenous mediator in the reaction mixture. In this bioconversion process, lactose is efficiently converted into lactobionic acid with a specific productivity of up to 3...
September 8, 2018: Letters in Applied Microbiology
Adina Catinca Grădinaru, Adriana Trifan, Adrian Şpac, Mihai Brebu, Anca Miron, Ana Clara Aprotosoaie
The aim of this study was to investigate the effects of ajowan essential oil/thymol and antibiotics combinations against three standard strains and six resistant clinical isolates of major respiratory bacteria (Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus pneumoniae). The broth microdilution method was conducted to determine the minimum inhibitory concentrations (MIC) of essential oil/thymol and antibiotics. The checkerboard method was used to investigate the interactions between the essential oil/thymol and antibiotics by means of the fractional inhibitory concentration index (FICI)...
September 6, 2018: Letters in Applied Microbiology
E Blázquez, C Rodríguez, J Ródenas, A Pérez de Rozas, J M Campbell, J Segalés, J Pujols, J Polo
The objectives of this study were to assess the effectiveness of an ultraviolet (UV-C, 254 nm) irradiation system and the spray-drying method as two independent safety steps on inactivation of Escherichia coli K88 and K99 spiked in porcine plasma at 6·46 ± 0·04 log10  ml-1 and 6·78 ± 0·67 log10  ml-1 respectively for UV-C method, and at 7·31 ± 0·39 log10  ml-1 and 7·66 ± 0·11 log10  ml-1 , respectively for the spray-drying method. The UV-C method was performed at different UV light doses (from 750 to 9000 J l-1 ) using a pilot plant UV-C device working under turbulent flow...
August 28, 2018: Letters in Applied Microbiology
Tao Yan, Fei Zhang, Yucheng He, Xiliang Wang, Xiue Jin, Panwang Zhang, Dingren Bi
Previously, we isolated a novel probiotic strain, designated HDRsEf1. In this study, we investigated the effects of this probiotic strain on intestinal barrier function and how it regulates the tight junction protein occludin in vitro. We used an ETEC-infected mouse model for the in vivo experiment. Briefly, 40 ICR mice were randomly divided into four groups: control group, assigned to saline gavage; prevention group, given HDRsEf1 before and saline after infection with ETEC; infection group, given saline both before and after infection with ETEC; treatment group, given saline before and HDRsEf1 after infection with ETEC...
August 28, 2018: Letters in Applied Microbiology
Hoa Quang Le, Elisabetta Suffredini, Dung Tien Pham, Anh Kim To, Dario De Medici
The detection of foodborne viruses in bivalve mollusks is a challenging procedure in relation to low virus concentration and to the presence of significant RT-PCR inhibitors. The aim of this study was the development of an efficient direct extraction method for foodborne viral RNA from bivalve mollusks. Using Mengovirus as a surrogate for foodborne viruses, five extraction methods based on RNA release by Trizol were compared on clams and oysters. A procedure consisting of Trizol, PureLink® RNA Mini Kit, followed by CTAB treatment and LiCl precipitation was found to provide RNA with highest extraction efficiency and negligible inhibitory effect on real-time RT-PCR...
August 24, 2018: Letters in Applied Microbiology
S A Burt, K Meijer, P Burggraaff, W S Kamerich, C Harmanus
Mice in buildings are a hygiene hazard because they harbour several zoonoses and animal diseases. The aim of this study was to gather information on specific bacteria in house mice caught in the urban environment. Mice caught in snap traps during pest control activities were collected in and around the city of Utrecht, the Netherlands, during May-June 2014, October-November 2015 and September-November 2016. The gut contents were analysed for ESBL/AmpC-producing Enterobacteriaceae, Salmonella spp., and Clostridium difficile and the buccal cavities were swabbed for methicillin-resistant S...
August 24, 2018: Letters in Applied Microbiology
Paulo S F Lichtemberg, Yong Luo, Herman Doussoulin, Themis J Michailides
Alternaria late blight caused by Alternaria alternata is a major disease affecting pistachios grown in California and to some degree those grown in Arizona. A. alternata is prone to quick fungicide resistance selection when single-mode of action fungicides are used. For the specific detection of five possible amino acid alterations associated with Alternaria alternata resistance to succinate dehydrogenase inhibitors used in California and Arizona pistachio orchards, we have designed five primer sets to be used as an allele-specific PCR assay (AS-PCR)...
August 24, 2018: Letters in Applied Microbiology
Piotr Cieślik, Józef P Knap, Lidia Piechowicz, Agata Bielawska-Drózd
Molecular epidemiology is one of the most rapidly developing research area. In the light of past and modern technologies it has gained number of typing methods based on molecular biology techniques. In this report, the subspecies differentiation of Francisella tularensis and genotyping of strains isolated in Poland and other geographic locations were investigated using real-time PCR and multispacer typing (MST) methods, respectively. In total, 49 strains of Francisella tularensis included 15 strains from Poland, for subspecies differentiation the real-time PCR method was applied...
August 14, 2018: Letters in Applied Microbiology
A K S Silva, T R N Silva, J R Nicoli, L M C Vasquez-Pinto, F S Martins
A healthy skin provides a protective barrier against pathogenic microorganisms. Recent studies have shown that probiotics, as those of Bifidobacterium genus, could act beneficially in dermatology, both when ingested and by topical use. In the present study, we evaluated by in vitro antagonism assays and using two skin cell lines the potential of four strains of Bifidobacterium spp. Among the four bifidobacteria, Bifidobacterium longum 51A was the only one able to inhibit the growth of the eight pathogenic indicators tested...
August 12, 2018: Letters in Applied Microbiology
S-Q Wang, T Wang, J-F Liu, L Deng, F Wang
Ergosterol biosynthesis in Saccharomyces cerevisiae is complex and the underlying mechanism of regulation remains unclear. To clarify the influence of transcriptional regulation on the ergosterol content, transcription factor Ecm22 was overexpressed in S. cerevisiae. Results showed that overexpression of ECM22 led to an increased invasive growth. Fluconazole susceptibility testing indicated that strains overexpressing ECM22 could grow at 20 μg(fluconazole) ml-1 . By contrast, the control failed to grow at 16 μg(fluconazole) ml-1 ...
August 11, 2018: Letters in Applied Microbiology
J Yang, M Y Gao, M Li, Z Z Li, H Li, H Y Li
The cotA gene from Bacillus amyloliquefaciens MN-13 was cloned and expressed in Escherichia coli Transetta. Nucleotide sequence analysis showed an open reading frame of 1542 bp encoding a polypeptide comprised of 513 amino acids. The degradation of lignin model compounds by recombinant CotA was investigated by HPLC-MS with guaiacylglycerol-β-guaiacyl ether as the substrate. The compounds including guaiacol, 3-(4-hydro-3-methoxyphenyl)-3-oxo-propanol and 4-hydro-3-methoxy acetophenone detected by HPLC-MS verified the rupture of β-O-4 bond and oxidation Cα bond of guaiacylglycerol-β-guaiacyl ether by CotA...
August 9, 2018: Letters in Applied Microbiology
Jun Yang, Changbo Tang, Jie Xiao, Wenfang Du, Rui Li
Citric acid and EGCG at their minimum inhibitory concentrations (MICs) were tested in this study. Logarithmic phase cells of E. coli O157:H7 (ATCC 43895) were exposed to EGCG and citric acid, respectively. The results of RT-real time PCR showed that both EGCG and citric acid increased stx2 and oxyR expression and decreased stx1, recA and Q expression. The result of western blotting for RecA protein further indicated that both EGCG and citric acid decreased RecA production. Both EGCG and citric acid increased the level of intracellular ROS and H2 O2 production and decreased SOD activity...
August 1, 2018: Letters in Applied Microbiology
C C Lazado, D Zilberg
Despite the significant development on their diagnoses and control, aeromonad infection is still a problem in aquaculture. This study described the key bacteriological and pathogenic features of a presumptive Aeromonas sp. isolated from the liver of a diseased guppy (Poecilia reticulata). Molecular identification revealed that the isolate was an Aeromonas veronii (A. veronii PR). It was able to grow in a wide range of temperatures and salt concentrations, and was capable of auto-aggregation and biofilm formation, with temperature as an influencing factor...
July 24, 2018: Letters in Applied Microbiology
M Marwa Abdel-Kareem, A A Zohri
Synthesis of gold nanoparticles (AuNPs) has become a needed domain of applied science. Biological method for synthesis of AuNPs by Trichoderma hamatum SU136 aqueous mycelial extract was achieved. The culture filtrate of the fungus was exposed to three different concentrations of gold chloride. The culture filtrate of the fungus was exposed to three different concentrations of gold chloride (0·25, 0·5 and 1·0 mmol l-1 ). In all cases, the gold ions (Au3+ ) were reduced to Au0 , leading to the formation of stable AuNPs...
July 20, 2018: Letters in Applied Microbiology
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