Read by QxMD icon Read

Letters in Applied Microbiology

Christopher Browne, Anette Loeffler, Hannah Holt, Yu-Mei Chang, David H Lloyd, Amanda Nevel
Porcine enzootic pneumonia (EP) caused by Mycoplasma hyopneumoniae adversely affects pig welfare and is associated with major economic losses in the pig industry worldwide. Transmission is predominantly by direct contact but the role of indirect transmission remains poorly understood. This study examined survival of six M. hyopneumoniae isolates dried onto five different surfaces encountered in pig units, and exposed to temperatures of 4°C, 25°C and 37°C for up to 12 days. Survival of the organisms was determined by recovering the organism from the surface material and culturing in Friis broth...
October 19, 2016: Letters in Applied Microbiology
Yangyang Ge, Junli Zhu, Xiaofeng Ye
Ten strains were isolated from a spoiled large yellow croaker (Pseudosciaena crocea). All of them were able to grow aerobically from 4 °C to 30 °C, and reduce trimethylamine N-oxide (TMAO) to trimethylamine (TMA) and produce H2 S except the SB01, PF05 and PF07. Biochemical characterization and phylogenetic analysis of 16S rRNA gene showed that eight H2 S producing isolates were closely related to Shewanella baltica, and two isolates PF05 and PF07 were identified as P. fluorescens and P. fragi, respectively...
October 16, 2016: Letters in Applied Microbiology
H W Kim, Y J Hong, J I Jo, S D Ha, S H Kim, H J Lee, M S Rhee
Microbiological quality of 206 raw ready-to-eat seafood samples were investigated according to species (gizzard shad, halibut, rockfish, tuna, oyster, and squid) and distribution channels (fishery, hyper, and online market). Enumeration of Aerobic Plate Count (APC) and total coliforms (TC) and pathogenic bacteria (Bacillus cereus, Staphylococcus aureus, and Vibrio parahaemolyticus) were performed, and level of microbiological quality were classified into four groups: satisfactory, acceptable, unsatisfactory, and unacceptable...
October 16, 2016: Letters in Applied Microbiology
Jian Cheng, Wei Zhuang, Nana Li, Chenglun Tang, Hanjie Ying
Low D-ribose production by B. subtilis was resulted from plenty of acid formation as responsible for carbon overflow. An approach of co-feeding glucose and sodium citrate is developed here and proved to be useful for D-ribose production. This strategy is critical because it affects the cell concentration, the productivity of D-ribose and, especially, the formation of by-products such as acetoin, lactate, and acetate. D-ribose production was increased by 59.6% from 71.06 to 113.41 g l(-1) without acid formation via co-feeding of 2...
October 14, 2016: Letters in Applied Microbiology
Bani K Pathak, Surojit Mondal, Chandana Barat
The ability of the ribosome to assist in folding of proteins both in vitro and in vivo is well documented and is a non-translational function of the ribosome. The interaction of the unfolded protein with the peptidyltransferase centre (PTC) of the bacterial large ribosomal subunit is followed by release of the protein in the folding competent state and rapid dissociation of ribosomal subunits. Our studies demonstrate that the PTC specific antibiotics, chloramphenicol and blasticidin S inhibit unfolded protein mediated subunit dissociation...
October 14, 2016: Letters in Applied Microbiology
Ja Hyun Lee, Hah Young Yoo, Xiaoguang Yang, Dong Sup Kim, Ju Hun Lee, Soo Kweon Lee, Sung Ok Han, Seung Wook Kim
In our previous study, glycerol was utilized as an additional carbon source for the production of cephalosporin C (CPC) by Acremonium chrysogenum M35. In this study, algal sugars extracted from the third generation biomass, were utilized in the CPC production for the first time. The CPC production improved about two-fold when using the algal sugars as the carbon source. The complex medium including algal sugars and glycerol was utilized, and 7.3 g l(-1) CPC production was achieved in a 250 mL shaking flask...
October 13, 2016: Letters in Applied Microbiology
Suresh Sadhasivam, Sekar Palanivel, Shamik Ghosh
Antimicrobials from natural sources have gained immense importance in recent times to combat the global challenge of antibiotic resistance. Essential oils are implicated in antimicrobial action against several species. Here we have screened nine commercially available essential oils for their antimicrobial activity against organisms associated with skin, scalp and nail infections mainly Propionibacterium acnes, Malassezia spp, Candida albicans and Trichophyton spp. Among nine essential oils, B. serrata essential oil demonstrated superior antimicrobial activity against all the microorganisms and surprisingly it showed maximum activity against Trichophyton spp...
October 12, 2016: Letters in Applied Microbiology
Jari Intra, Roberta Maria Sala, Rosanna Falbo, Fabrizio Cappellini, Paolo Brambilla
Rapid and early identification of microorganisms in blood has a key role in the diagnosis of a febrile patient, in particular, in guiding the clinician to define the correct antibiotic therapy. This study presents a simple and very fast method with high performances for identifying bacteria by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) after only 4 hours of incubation. We used early bacterial growth on PolyViteX chocolate agar plates inoculated with 5 drops of blood-broth medium deposited in the same point and spread with a sterile loop, followed by a direct transfer procedure on MALDI-TOF MS target slides without additional modification...
October 12, 2016: Letters in Applied Microbiology
Walter P Pfliegler, Matthias Sipiczki
Simple and efficient genotyping methods are widely used to assess the diversity of a large number of microbial strains, e.g. wine yeasts isolated from a specific geographic area or a vintage, etc. Such methods are often also the first to be applied, to decrease the number of strains deemed interesting for a more time-consuming physiological characterization. Here, we aimed to use a physiologically characterized strain collection of 69 S. cerevisiae strains from Hungarian wine regions to determine whether geographic origin or physiological similarity can be recovered by clustering the strains with one or two simultaneously used variations of interdelta genotyping...
October 8, 2016: Letters in Applied Microbiology
Jenni Inkinen, Riika Mäkinen, Minna M Keinänen-Toivola, Katrina Nordström, Merja Ahonen
The present study was performed in real life settings in different facilities (hospital, kindergarten, retirement home, office building) with copper and copper alloy touch surface products (floor drain lids, toilet flush buttons, door handles, light switches, closet touch surfaces, corridor hand rails, front door handles, and toilet support rails) parallel to reference products. Pure copper surfaces supported lower total bacterial counts (16 ± 45 vs 105 ± 430 CFU cm(-2) , N = 214, P < 0.001) and lower occurrence of Staphylococcus aureus (2...
October 8, 2016: Letters in Applied Microbiology
Yi Li, Lei Liu, Yanting Xu, Li Ping, Kun Zhang, Xiaobing Jiang, Tianling Zheng, Hailei Wang
Microcystis aeruginosa is a cyanobacterial bloom-causing species and has been considered a serious human health and biological safety threat. In this study, algicidal bacterium h10 showed high algicidal effects on M. aeruginosa 7820, and strain h10 was confirmed to belong to genus Exiguobacterium, for which name Exiguobacterium sp. h10 is proposed. Algicidal activity and mode analysis revealed that supernatant rather than bacterial cells was responsible for showing algicidal activity, which indicated that the algicidal mode of strain h10 is an indirect attack through producing algicidal substances...
October 7, 2016: Letters in Applied Microbiology
Emilia Matallana, Agustín Aranda
Wine yeast deals with many stress conditions during its biotechnological use. Biomass production and its dehydration produce major oxidative stress, while hyperosmotic shock, ethanol toxicity and starvation are relevant during grape juice fermentation. Most stress response mechanisms described in laboratory strains of Saccharomyces cerevisiae are useful for understanding the molecular machinery devoted to deal with harsh conditions during industrial wine yeast uses. However, the particularities of these strains themselves, and the media and conditions employed, need to be specifically looked at when studying protection mechanisms...
October 7, 2016: Letters in Applied Microbiology
Ming-Yi Wang, Jian-Li Geng, Ying-Jian Chen, Yu Song, Mei Sun, Hai-Zhu Liu, Cheng-Jin Hu
Methicillin-resistant staphylococci and ESBL-producing bacteria are the most important resistant pathogens in sepsis. In the study, a new multiplex-touchdown PCR method (MT-PCR) was developed to detect rapidly and simultaneously the presence of mecA, blaSHV , blaCTX-M , blaTEM and blaOXA genes from positive blood culture bottles. The technique showed a sensitivity of 10(3) CFU ml(-1) for mecA detection and of 10(2) CFU ml(-1) for other genes, and 100% specificity in the detection of all genes. All genes were detected in the spiked blood culture bottles artificially contaminated with reference strains...
October 4, 2016: Letters in Applied Microbiology
Ilada Choopara, Narong Arunrut, Wansika Kiatpathomchai, Deborah Dean, Naraporn Somboonna
We developed an assay comprising crude DNA lysis by simple heat treatment coupled loop-mediated isothermal amplification with hydroxynaphthol blue (LAMP-HNB) for C. trachomatis detection, and evaluated the developed assay for its feasibility as one-step point-of-care detection on 284 endocervical swab specimens from clinically symptomatic C. trachomatis and healthy subjects. This assay is sensitive to 0.04 pg of ompA, specific with 6 primers targeting C. trachomatis ompA region, rapid (45 min total assay time), inexpensive (~3 USD/reaction), does not require sophisticated instrumentation, and has comparable assay effectiveness (95% specificity, 90-100% sensitivity) to the bacterial DNA isolation by commercial kit coupled with PCR and gel electrophoresis (98-100% specificity, 87-100% sensitivity) based on the clinical samples test...
September 30, 2016: Letters in Applied Microbiology
Y Yang, M Kitajima, T P T Pham, L Yu, R Ling, K Y H Gin, M Reinhard
Hydrogen peroxide (H2 O2 ) is widely used in water treatment for biofouling control and, in conjunction with catalysts, as a powerful oxidant for contaminant destruction. H2 O2 could potentially serve as an anti-fouling agent in reverse osmosis systems in lieu of chlorine-based disinfectants. The dependence of the biocidal efficiency of H2 O2 on cell density, temperature and H2 O2 concentration by determining the growth, attachment and viability of the model bacterium P. aeruginosa PAO1 was studied. For controlling growth of planktonic PAO1 cells, the minimally required H2 O2 concentration depends on the cell density and temperature...
September 28, 2016: Letters in Applied Microbiology
Zhi-Jie Li, Xiao-Ye Shen, Cheng-Lin Hou
A total of 374 fungal endophyte strains were isolated from of Vaccinium dunalianum var. urophyllum (Ericaceae), a well-known cultivated blueberry in southern China. These fungal endophytes could be categorized into 25 morphotypes according to culture characteristics and molecular identification based on the internal transcribed spacer (ITS) region. All of these isolates belonged to Ascomycota. Jaccard's (Jc) and Sorenson's similarity indices indicated that the species communities from the fruits and branches were closer to each other than to those from leaves...
September 23, 2016: Letters in Applied Microbiology
M Ben Said, Abbassi M S, V Bianchini, S Sghaier, P Cremonesi, A Romanò, V Gualdi, Hassen A, M Luini
Staphylococcus aureus is a major agent of bovine mastitis in dairy herds, causing economic losses in dairy industry worldwide. In addition, milk and milk-products contaminated by Staph. aureus can cause harmful human diseases. The aim of this study was to characterize Staph. aureus strains isolated from dairy farms in Tunisia. Bulk tank milk (n = 32) and individual cow's milk (n = 130) samples were collected during the period of 2013-2014. Forty-three S. aureus isolates were recovered and typed by spa typing, 16S-23S rRNA intergenic spacer (RS-PCR) and multiplex PCRs for 22 virulence genes...
September 22, 2016: Letters in Applied Microbiology
Jonathan S McQuillan, David J Hopper, Iordanis Magiopoulos, Martin Arundell, Robin Brown, Steven Shorter, Matthew C Mowlem, Robin W Pascal, Douglas Connelly
Bio-fouling is a process of ecological succession which begins with the attachment and colonisation of microorganisms to a submerged surface. For marine sensors and their housings, bio-fouling can be one of the principle limitations to long-term deployment and reliability. Conventional anti-bio-fouling strategies using biocides can be hazardous to the environment, and therefore alternative chemical-free methods are preferred. In this study, custom made testing assemblies were used to evaluate ultrasonic vibration as an anti-bio-fouling process for marine sensor housing materials over a 28-day time course...
September 22, 2016: Letters in Applied Microbiology
Shahram Shafaei, Jörg Dörrstein, Josef Peter Guggenbichler, Cordt Zollfrank
The objective of this research is to develop novel cellulose acetate (biopolymer) composite materials with an excellent antimicrobial activity by embedding molybdenum trioxide particles with unique high specific surface area. High surface area molybdenum trioxide particles were prepared from freshly precipitated molybdenum trioxide dihydrate (MoO3 x2H2 O) and subsequent calcination at 340 °C under H2 /N2 gas. Microbiological evaluation against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were performed applying a roll-on test and excellent antimicrobial activities were determined for composites with embedded anhydrous molybdenum trioxide with a high specific surface area...
September 20, 2016: Letters in Applied Microbiology
Seyedesomaye Jasemi, Masoumeh Douraghi, Habibeh Adibhesami, Hojjat Zeraati, Mohammad Rahbar, Mohammad Ali Boroumand, Amir Aliramezani, Sedighe Ghourchian, Mona Mohammadzadeh
Comprehensive data on drug resistant patterns of Acinetobacter baumannii isolates in developing countries is limited. We conducted a multi-hospital study to assess the rate and trend of drug-resistant phenotypes in A. baumannii using standardized definitions and to determine the remaining therapeutic options against resistant phenotypes. The 401 non-duplicate isolates were collected from 6 hospitals which are geographically distributed across Tehran, Iran over a three year period. Following PCR of blaOXA-51-like gene, susceptibility testing was performed against 9 antimicrobial agent categories...
September 14, 2016: Letters in Applied Microbiology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"