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Letters in Applied Microbiology

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https://www.readbyqxmd.com/read/28517029/carbon-driven-enrichment-of-the-crucial-nitrate-reducing-bacteria-in-limed-peat-soil-microcosms
#1
Ying Zhu, Xiaoyuan Zhang, Xiaogang Wu, Guanzhou Chen, Lars R Bakken, Liping Zhao, Åsa Frostegård, Xiaojun Zhang
Bacteria of Dechloromonas were recognized as potential functional important denitrifiers in a long term shell sand amended peat soil. Different microcosms in a solid matrix and slurry systems with the addition of carbon and nitrogen sources, e.g., clover leaves, glutamate, and nitrate, were established. The bacterial community structures were analyzed by pyrosequencing of the 16S rRNA gene to select the conditions for enriching bacteria of Dechloromonas. The results showed that a relatively even bacterial community in the initial soil shifted to communities dominated by a few types of nitrate reducing bacteria after the incubation, which strongly responded to the carbon substrates addition and consumption...
May 18, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28504828/improvement-in-solvent-tolerance-by-exogenous-glycerol-in-pseudomonas-sp-bcnu-106
#2
Hye Jung Choi, Bo Ra Lim, Yeo Jin Park, Woo Hong Joo
Solvent hyper-tolerant Pseudomonas sp. BCNU 106 still has some underlying growth limitation in solvents. Therefore, efficient mass cultivation methods are needed to pursue its applications in biotechnology. Pseudomonas sp. BCNU 106 was cultured in a medium supplemented with 0.05 mol l(-1) glycerol and cell survival was monitored during its cultivation in the presence of 1% (v/v) toluene. Exogenously-supplemented glycerol provided more protection against damage caused by toluene stress and conferred higher solvent tolerance of Pseudomonas sp...
May 15, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28504825/one-step-simultaneous-detection-of-ureaplasma-parvum-and-genotypes-sv1-sv3-and-sv6-from-clinical-samples-using-plexpcr-technology
#3
Matthew S Payne, Lucy L Furfaro, Rachel Tucker, Lit Y Tan, Elisa Mokany
Ureaplasma spp. are associated with preterm birth. In recent times, it has become apparent that U. parvum, but not U. urealyticum, is of most relevance. We recently demonstrated this in Australian pregnant women and using high-resolution melt (HRM) PCR, further showed that U. parvum genotype SV6 was of particular significance. However, our assay was unable to identify multiple genotypes in the same sample, required a separate species-level qPCR for low titre samples and was not ideal for diagnostic laboratories due to the nature of HRM PCR result interpretation...
May 15, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28493279/specific-detection-of-gii-1-lineage-of-infectious-bronchitis-virus
#4
Katarzyna Domanska-Blicharz, Anna Lisowska, Anna Pikuła, Joanna Sajewicz-Krukowska
Infectious bronchitis virus (IBV) is a worldwide prevalent RNA virus that cause highly contagious and economically devastating disease in chicken. The virus exists in many different genetic forms which made the disease control very difficult. The present study describes the development and validation of TaqMan-probe-based real-time reverse transcription-polymerase chain reaction (real time RT-PCR) targeting the S1 coding region of S gene characteristic for the GII-1 lineage (formerly the D1466-like variant) of IBV...
May 10, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28474344/differential-expression-of-duplicated-binary-toxin-genes-bina-binb-in-lysinibacillus-sphaericus-c3-41
#5
Pan Fu, Xingchen Xiang, Yong Ge, Zhiming Yuan, Xiaomin Hu
Due to the self-produced binary toxins BinA/BinB, some Lysinibacillus sphaericus isolates exhibit high toxicity against mosquito larvae and are utilized in mosquito control programs. Previous whole genome sequencing revealed the presence of a large plasmid pBsph in L. sphaericus C3-41, which contains a 30.5-kb duplication of the genome including the binary toxin genes binA/binB. This was confirmed by Southern blot and qPCR experiments in this study. Mutants of L. sphaericus C3-41, bearing disruptions of the chromosomal-bin (C3-41ΔCab ) or the plasmid-bin (C3-41ΔPab ) and a plasmid cured strain G725, were generated...
May 5, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28444877/identification-and-measurement-of-staphylococcal-enterotoxin-m-sem-from-staphylococcus-aureus-isolate-associated-with-staphylococcal-food-poisoning
#6
Yanying Zhao, Anni Zhu, Junni Tang, Cheng Tang, Juan Chen
Staphylococcus aureus (S. aureus) produces a wide variety of staphylococcal enterotoxins (SEs, SEA to SEX), which are responsible for staphylococcal food poisoning. This study is aimed to establish a system to detect SEM protein in food matrixes. In the present study, sem gene was characterized in a S. aureus isolate H4 associated with food poisoning. The amino acid sequence of the deduced SEM protein was same as that of previously identified SEM from S. aureus 04-02981. Subsequently, mature SEM protein was expressed in E...
April 26, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28434202/distribution-of-norovirus-genotypes-and-subtypes-in-river-water-by-ultra-deep-sequencing-based-analysis
#7
M Boonchan, K Motomura, K Inoue, H Ode, P Y Chu, M Lin, Y Iwatani, K Ruchusatsawat, R Guntapong, R Tacharoenmuang, S Chantaroj, M Tatsumi, N Takeda, S Sangkitporn
To determine the distribution of NoV genotypes in natural river water in Thailand, we conducted a genome analysis using a next-generation sequencer. Twenty-five river water samples were collected at five different sites of the Khlong Klon River in the suburbs of Bangkok between August 2013 and December 2014. The partial genome of NoV was detected in 15 of the 25 samples (60.0%). Seven of these 15 samples (46.7%) contained multiple NoV GII genotypes: GII.4, GII.6, and GII.17. Our data showed that GII.17 had already emerged in August 2013 as a minor population, and it became a major genotype in December 2014...
April 23, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28426165/hydrogen-peroxide-dependent-antibacterial-action-of-melilotus-albus-honey
#8
Patrycja Sowa, Dorota Grabek-Lejko, Monika Wesołowska, Sylwia Swacha, Małgorzata Dżugan
Honey originating from different floral sources exhibits the broad-spectrum of antibacterial activity as a result of the presence of hydrogen peroxide as well as non peroxide bioactive compounds. The mechanisms of antibacterial activity of Polish melilot honey were investigated for the first time. Polish melilot honey samples (Melilotus albus biennal -3 and annual-5, Melilotus officinalis -1) were collected directly from beekeepers and analyzed for pollen profile, basic physicochemical parameters, antioxidant capacity, radical scavenging activity, total phenolics contents as well as antibacterial properties against pathogenic bacteria Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Salmonella spp...
April 20, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28421612/prospective-bacterial-quorum-sensing-inhibitors-from-indian-medicinal-plant-extracts
#9
Bipransh Kumar Tiwary, Runu Ghosh, Saurav Moktan, Vivek Kumar Ranjan, Priyankar Dey, Dibakar Choudhury, Somit Dutta, Dipanwita Deb, Abhaya Prasad Das, Ranadhir Chakraborty
As virulence of many pathogenic bacteria is regulated by the phenomenon of quorum sensing (QS), the present study aimed to find the QS-inhibiting (QS-I) property (if any) in sixty-one Indian medicinal plants. The presence of QS-I compound in the leaf-extract was evaluated by its ability to inhibit production of pigment in Chromobacterium violaceum MTCC 2656 (violacein) and Pseudomonas aeruginosa MTCC 2297 (pyocyanin) or swarming of P. aeruginosa MTCC 2297. Extracts of three plants, Astilbe rivularis, Fragaria nubicola and Osbeckia nepalensis have shown a dose- dependent inhibition of violacein production with no negative effect on bacterial growth...
April 19, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28418627/prokaryote-species-richness-is-positively-correlated-with-eukaryote-abundance-in-wastewater-treatment-biofilms
#10
Inga Leena Angell, Jon Fredrik Hanssen, Knut Rudi
Biological treatment represents a key step in nutrient removal from wastewater. Until now these process has mainly been considered prokaryotic, with the interactions between prokaryotes and eukaryotes not being properly explored. We therefore investigated the co-occurrence of eukaryotes and prokaryotes in biological nitrogen removal biofilms. We found that biofilms in the nitrifying reactor contained the highest diversity and abundance of both prokaryotes and eukaryotes, with nearly three times higher prokaryote species richness than for the denitrifying reactor...
April 18, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28418590/control-of-biofouling-by-xanthine-oxidase-on-seawater-reverse-osmosis-membranes-from-a-desalination-plant-enzyme-production-and-screening-of-bacterial-isolates-from-the-full-scale-plant
#11
Veena Nagaraj, Lucy Skillman, Dan Li, Zhangwang Xie, Goen Ho
Control of biofouling on seawater reverse osmosis (SWRO) membranes is a major challenge as treatments can be expensive, damage the membrane material and often biocides do not remove the polymers in which bacteria are embedded. Biological control has been largely ignored for biofouling control. The objective of this study was to demonstrate the effectiveness of xanthine oxidase enzyme against complex fouling communities and then identify naturally occurring bacterial strains that produce the free radical generating enzyme...
April 18, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28419596/effects-of-culture-conditions-on-the-size-morphology-and-wet-density-of-spores-of-bacillus-cereus-569-and-bacillus-megaterium-qm-b1551
#12
Ke Xu Zhou, Florencia Wisnivesky, D Ian Wilson, Graham Christie
The influence of variable culture conditions on the size and wet density of spores of Bacillus cereus and Bacillus megaterium were examined in this work. Culture temperature and initial pH was shown to have a significant impact on the size of both species, with increasingly alkaline culture media and elevated culture temperatures resulting in spores that were, on average, up to 25% reduced in volume. Increasing concentrations of inorganic salts in sporulation media exerted differing effects on each species; whereas a five-fold increase in the concentration of all salts resulted in only minor differences to the dimensions of B...
April 17, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28419503/comparison-of-the-quantitative-dry-culture-methods-with-both-conventional-media-and-most-probable-number-method-for-the-enumeration-of-coliforms-and-escherichia-coli-coliforms-in-food
#13
Hajime Teramura, Kojiro Sota, Mihoko Iwasaki, Hirokazu Ogihara
Sanita-kun(™) CC (coliform count) and EC (Escherichia coli/coliform count), sheet quantitative culture systems which can avoid chromogenic interference by lactase in food, were evaluated in comparison with conventional methods for these bacteria. Based on the results of inclusivity and exclusivity studies using 77 microorganisms, sensitivity and specificity of both Sanita-kun(™) met the criteria for ISO 16140. Both media were compared with deoxycholate agar, violet red bile agar, Merck Chromocult(™) coliform agar (CCA), 3M Petrifilm(™) CC and EC (PEC), and 3-tube MPN, as reference methods, in 100 naturally contaminated food samples...
April 17, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28397273/internalisation-of-escherichia-coli-o157-h7-gfp-in-rocket-and-swiss-chard-baby-leaves-as-affected-by-abiotic-and-biotic-damage
#14
Rahel Hartmann, Andreas Fricke, Hartmut Stützel, Suzan Mansourian, Teun Dekker, Walter Wohanka, Beatrix Alsanius
Internalisation of human pathogens in edible parts of vegetables eaten raw is a major concern, since once internalised they are protected from sanitising treatments. In this study, we examined invasion of gfp-labelled E. coli O157:H7 into intact and biotically (infection with Xanthomonas campestris/Pseudomonas syringae) and abiotically (grating with silicon carbide) damaged leaves of wild rocket (Diplotaxis tenuifolia) and Swiss chard (Beta vulgaris subsp. cicla) using laser scanning confocal microscopy. Bacterial cells were found in internal locations of the tissue, irrespective of tissue health status...
April 11, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28394467/comparative-isolation-and-genetic-diversity-of-arcobacter-spp-from-fish-and-the-coastal-environment
#15
Srinu Rathlavath, Sanath Kumar, Binaya Bhusan Nayak
Arcobacter species are emerging food-borne and water-borne human pathogens associated mostly with food animals and their environment. The present study was aimed to isolate Arcobacter species from fish, shellfish and coastal water samples using two methods and to determine their genetic diversity. Of 201 samples of fish, shellfish and water samples analysed, 66 (32.8%) samples showed the presence of Arcobacter DNA from both Arcobacter enrichment broth (AEB) and Bolton broth (BB). Arcobacters were isolated from 58 (87...
April 10, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28378421/fermented-cereal-beverages-from-probiotic-prebiotic-and-synbiotic-towards-nanoscience-designed-healthy-drinks
#16
REVIEW
Ivan Salmerón
The consumption of fermented food by human kind goes a long way back in history and there are as many types of fermented food as civilizations. Food science and technology has progressed from designing nutritional food towards food with health improvement characteristics such as functional foods. In this sense, the area of food with properties to improve gastrointestinal health like probiotics, prebiotics and synbiotics has been the most important segment within functional foods. Most of these products are dairy-based so the development of non-dairy gut improvement products has been of great interest for the food industry, resulting in the rise of cereal-based probiotic and synbiotic products...
April 4, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28304098/bacteriophages-as-indicators-of-fecal-pollution-and-enteric-virus-removal
#17
REVIEW
Brian R McMinn, Nicholas J Ashbolt, Asja Korajkic
Bacteriophages are an attractive alternative to fecal indicator bacteria (FIB), particularly as surrogates of enteric virus fate and transport, due to their closer morphological and biological properties. Based on a review of published data, we summarize densities of coliphages (F+ and somatic), Bacteroides spp. and enterococci bacteriophages (phages) in individual human waste, raw wastewater, ambient fresh and marine waters and removal through wastewater treatment processes utilizing traditional treatments...
March 17, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28276074/survival-of-escherichia-coli-in-common-garden-mulches-spiked-with-synthetic-greywater
#18
S Boyte, S Quaife, J Horswell, A Siggins
Reuse of domestic wastewater is increasingly practiced as a means to address global demands on fresh water. Greywater is primarily reused via subsurface irrigation of gardens, where the soil environment is seen to be an integral part of the treatment process. The fate of biological contaminants (i.e. pathogens) in the soil is reasonably well understood, but their persistence and survival in soil cover layers is largely unexplored. This study investigated the ability of Escherichia coli to survive in common soil cover layers...
March 9, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28276067/in-vitro-and-in%C3%A2-vivo-evaluation-of-weissella-cibaria-and-lactobacillus-plantarum-for-their-protective-effect-against-cadmium-and-lead-toxicities
#19
O Ojekunle, K Banwo, A I Sanni
Thirty-two lactic acid bacteria (LAB) isolates were obtained from fermenting cassava mash and wara (African soft cheese) and screened for their resistance to cadmium and lead toxicities at 550-1050 mg l(-1) and probiotic potentials. Four LAB strains that tolerated the heavy metals at 1050 mg l(-1) were selected for antioxidative capacities, tolerance to acid, bile salts and simulated gastric and intestinal tract and safety status. The results revealed that Weissella cibaria WD2 and Lactobacillus plantarum CaD1 exhibited comparatively higher antioxidative capacities, survived in simulated gastric and intestinal transit, tolerated acid and bile salt and possessed safety status...
March 9, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28266721/biodesulphurization-of-gasoline-by-rhodococcus-erythropolis-supported-on-polyvinyl-alcohol
#20
A Fatahi, S Sadeghi
A new biodesulphurization (BDS) method has been considered using Rhodococcus erythropolis supported on polyvinyl alcohol (PVA) for BDS of thiophene as a gasoline sulphur model compound in n-hexane as the solvent, subsequently this biocatalyst has been applied to BDS of gasoline samples. The obtained results according to UV-Spectrophotometer analysis at 240 nm showed that 97·41% of thiophene at the optimum condition of primary concentration 80 mg l(-1) , pH = 7, by 0·1 g of biocatalyst in 30°C and after 20 h of contact time has been degraded...
March 7, 2017: Letters in Applied Microbiology
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