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Analytical and Quantitative Cytology and Histology

Priya Thomas, Pratibha Ramani, Priya Premkumar, Anuja Natesan, Herald J Sherlin, Thiruvengadam Chandrasekar
OBJECTIVE: To evaluate DNA damage and cellular death in exfoliated buccal mucosa cells from healthy individuals following dental X-ray exposure, to compare effects of different types of radiographic techniques using the buccal cytome assay and to standardize the staining procedure. STUDY DESIGN: This study comprised 90 patients, categorized into 3 groups subjected to intraoral periapical radiography, conventional orthopantomogram and digital orthopantomogram X-ray exposure...
June 2012: Analytical and Quantitative Cytology and Histology
Gilbert Bigras
OBJECTIVE: Color deconvolution relies on determination of unitary optical density vectors (OD(3D)) derived from pure constituent stains initially defined as intensity vectors in RGB space. OD(3D) can be defined in polar coordinates (phi, theta, radius); always being equal to one, radius can be ignored. Easier handling of unitary optical density 2D vectors (OD(2D)) is shown. STUDY DESIGN: OD(2D) pure stains used in anatomical pathology were assessed as centroid values (phi, theta) with a measure of variance: inertia based on arc lengths between centroid value and sampled points...
June 2012: Analytical and Quantitative Cytology and Histology
Ayhan Ozcan, Bulent Kurt, Yildirim Karslioglu, Ali Hakan Cermik, Alp Bilal Firat
OBJECTIVE: To assess the potential contribution of nuclear morphometry to the differential diagnosis of renal epithelial tumors with eosinophilic cytoplasm, including chromophobe renal cell carcinoma (ChRCC), the eosinophilic variant of clear cell renal cell carcinoma (EoRCC), and oncocytoma. STUDY DESIGN: A total of 24 tumor tissue samples diagnosed as ChRCC, the EoRCC, or oncocytoma constituted our series. Eight geometric features such as nuclear area, nuclear perimeter, and circular form factor were measured and compared among the groups...
June 2012: Analytical and Quantitative Cytology and Histology
Roberta Mazzucchelli, Marina Scarpelli, Antonio Lopez-Beltran, Liang Cheng, Rodolfo Montironi
There exists a wide and heterogeneous spectrum of spindle cell lesions that can originate from the prostatic stroma. This includes benign and malignant neoplasms as well as tumorlike conditions. Their diagnosis in small biopsy specimens is often difficult because of the potential for sampling error. The utility of ancillary studies, including immunohistochemistry, is often limited and the main criteria for diagnosis are the morphologic findings by routine hematoxylin and eosin stain.
June 2012: Analytical and Quantitative Cytology and Histology
Mehrnoush Khojasteh, Timon P H Buys, Jean LeRiche, Stephen Lam, Martial Guillaud, Calum MacAulay
OBJECTIVE: Deregulated cell proliferation is a hallmark of cancer, and Ki67 immunostaining can be used to identify proliferating cells. Evaluation of cell proliferation may have utility as a biomarker of epithelial malignant transformation risk. To date, most analyses of Ki67 staining have been restricted to semiquantitative estimations of the degree of staining or the measurement of the fraction of Ki67-positive cells within the epithelium. We sought to develop a robust, objective means of quantitatively evaluating Ki67 immunostaining for lung precancerous lesions...
June 2012: Analytical and Quantitative Cytology and Histology
Roberta Mazzucchelli, Liang Cheng, Antonio Lopez-Beltran, Marina Scarpelli, Rodolfo Montironi
The classification and grading of the noninvasive, intraepithelial neoplasms of the urothelium are based on the morphological pattern of growth, i.e., papillary and flat (and endophytic), and on their degree of architectural and cytologic abnormalities. Recent advances in the morphological, molecular and quantitative evaluation of these lesions have contributed to the refinement of the current classification and grading schemes. However, some controversies on the precise criteria and terminology, especially when the papillary lesions are concerned, are still present...
June 2012: Analytical and Quantitative Cytology and Histology
Alessandra Filosa, Andrea Fabiani
No abstract text is available yet for this article.
April 2012: Analytical and Quantitative Cytology and Histology
Adarsh Barwad, Pranab Dey
OBJECTIVE: To introduce a new field of multifractal spectrum in distinguishing between endometrial well-differentiated adenocarcinoma (WDAC) and complex atypical hyperplasia (CAH). STUDY DESIGN: Thirteen cases of CAH and 16 of WDAC were selected from radical hysterectomy specimens, and multifractal spectrum was measured from at least 4-5 representative digitized images of each case. The data were collected from f(alpha) vs. alpha curves. The values of alpha max, alpha min, and their difference delta alpha (alpha max-alpha min) were recorded and the data compared...
April 2012: Analytical and Quantitative Cytology and Histology
Enrico Bollito, Stefano De Luca, Matteo Cicilano, Roberto Passera, Susanna Grande, Carmen Maccagnano, Susanna Cappia, Angela Milillo, Francesco Montorsi, Roberto Mario Scarpa, Mauro Papotti, Donato Franco Randone
OBJECTIVE: To determine an optimal prostate cancer gene 3 (PCA3) cutoff in predicting prostate cancer in Italian patients undergoing first or repeat biopsy. STUDY DESIGN: In this observational multicenter study 1246 men with elevated prostate specific antigen (PSA) and negative digital rectal examination, with prostate biopsy after PCA3 assessment, were divided into two groups submitted to PCA3 testing before or after previous negative biopsies. Ideal PCA3 cutoff was identified using area under the curve of the receiver operating characteristic analysis...
April 2012: Analytical and Quantitative Cytology and Histology
Vladimir Kirillov, Olga Emeliyanova
OBJECTIVE: To develop expert systems for classification of follicular thyroid tumor at a preoperative stage. STUDY DESIGN: Fine needle aspiration biopsy of the thyroid gland with a histologic conclusion of follicular cancer and follicular adenoma were the object of the morphometric study. General sample size was 4500 nuclei and 3000 aggregates. RESULTS: Quantitative regularities of pathologic changes in thyrocyte nuclei and aggregates in follicular cancer and follicular adenoma were revealed...
April 2012: Analytical and Quantitative Cytology and Histology
Asmaa Gaber Abdou, Shawky El Farargy, Iman Seleit, Azaa Gaber Antar, Mohammed Elhefny, Nada Farag Elnaidany
OBJECTIVE: To explore the possible role of osteopontin (OPN) in chronic plaque psoriasis and understand the role of inflammation in psoriasis. STUDY DESIGN: We investigated 36 patients with chronic plaque psoriasis using immunohistochemical technique for evaluation of OPN immunolocalization compared to normal skin biopsies of 10 normal subjects representing the control group. RESULTS: OPN was expressed in the epidermis of all specimens, both in the psoriasis group and the control group without any significant differences except for the tendency of psoriatic lesions to show more cytoplasmic and nuclear pattern of OPN staining (55...
April 2012: Analytical and Quantitative Cytology and Histology
Rossana Berardi, Alfredo Santinelli, Azzurra Onofri, Alessandro Brunelli, Chiara Pierantoni, Eleonora Pisa, Alessandra Pagliacci, Daniela Stramazzotti, Lina Zuccatosta, Paola Mazzanti, Armando Sabbatini, Stefano Gasparini, Italo Bearzi, Stefano Cascinu
OBJECTIVE: To evaluate prognostic impact of maspin expression in patients with resected non-small cell lung cancer (NSCLC). STUDY DESIGN: From 1996 to 2001, 439 patients underwent radical surgery for NSCLC at the Polytechnic University of the Marche Region. Maspin expression was detected as cytoplasmic and nuclear staining of neoplastic cells. For cytoplasmic staining, cases were classified as negative, low positive, and high positive. In positive cases, intensity of staining was also considered and scored...
April 2012: Analytical and Quantitative Cytology and Histology
Jorge Ernesto González, Manual Lee, Joan Francesc Barquinero, Marco Valente, Sandrine Roch-Lefèvre, Omar García
OBJECTIVE: To test a CellProfiler pipeline for automated counting and characterization of gamma-H2AX foci in color images of human cultured cells. STUDY DESIGN: A431 cells were irradiated and stained for gamma-H2AX foci detection. Sets of color images were analyzed visually, and findings were compared with those using FociCounter and CellProfiler software. RESULTS: The CellProfiler pipeline includes some proprieties not present in FociCounter, such as the automatic detection of nuclei, the detection of touching nuclei and the rejection of nuclei that touch the border of the image...
April 2012: Analytical and Quantitative Cytology and Histology
Antonio Lopez-Beltran, Liang Cheng, Ana Blanca, Rodolfo Montironi
OBJECTIVE: To analyze whether cell proliferation and apoptotic indexes in needle biopsies with prostate cancer Gleason 6 or 7 can identify more objectively Gleason score 6 or 7 in needle biopsy samples. STUDY DESIGN: Seventy patients diagnosed by needle biopsy and treated by radical prostatectomy were included. Fifty cases were Gleason score 6 and 20 were Gleason 7. Twenty-two cases were organ-confined and 48 nonorgan-confined. Histologic sections from needle biopsies were stained for cell proliferation using the MIB 1 (Ki67) antibody and in situ end-labeling technique for apoptosis and recorded as the percentage of positive cells...
April 2012: Analytical and Quantitative Cytology and Histology
Shelly Sharma, Pranab Dey, Uma Nahar Saikia, Raje Nijhawan
OBJECTIVE: To evaluate the role of micronuclei (MN) scoring in urinary cytology of bladder transitional cell carcinoma (TCC). STUDY DESIGN: Eighty cases of freshly voided urine samples were used for scoring of MN using acridine orange (AO)-stained smears under fluorescence microscope and routine Papanicolaou-stained smears under light microscopy. MN score for cytologic lesions was compared. RESULTS: In AO-stained slides, the mean MN scores were 0...
February 2012: Analytical and Quantitative Cytology and Histology
Pablo Conesa-Zamora, Javier Trujillo-Santos, Francisco Javier Orantes-Casado, Sebastián Ortiz-Reina, Daniel Torres-Moreno, Miguel Pérez-Guillermo
OBJECTIVE: To study the performance values of a set of five immunohistochemical markers involved in cell cycle regulation as a potential aid in the differential diagnosis between squamous intraepithelial lesions (SILs) and normal or benign conditions of the uterine cervix. STUDY DESIGN: Results from immunohistochemical evaluation of p16, cyclin D1, p53, Ki67, and ProEx C markers and human papillomavirus genotyping were collected from a previous study performed on 37 normal or benign cervices, 39 low grade SILs and 73 high grade SILs...
February 2012: Analytical and Quantitative Cytology and Histology
Yeong-Hui Kim, Ga-Eon Kim, Ji Shin Lee, Jae Hyuk Lee, Jong Hee Nam, Chan Choi, Min Ho Park, Jung Han Yoon
OBJECTIVE: To ascertain the hormonal receptor profiles of the epithelial and stromal components of phyllodes tumors (PTs) and determine their relationship with stromal proliferation. STUDY DESIGN: Eighty-two PTs (50 benign, 22 borderline, and 10 malignant) were studied. Automated immunohistochemical staining for estrogen receptor (ER)-alpha and -beta, progesterone receptor (PR), androgen receptor (AR), and Ki-67 was performed using tissue microarray blocks, and their expression was assessed in both the stromal and epithelial components...
February 2012: Analytical and Quantitative Cytology and Histology
Ian S Hagemann, Theresa L Pasha, Shelley A Roberts, Sabrina W Yum, Paul J Zhang
OBJECTIVE: To determine whether connexin (Cx) expression is altered in cervical dysplasia. STUDY DESIGN: Cx proteins form gap junctions and are expressed in squamous epithelia including ectocervix. We used multispectral imaging to perform a quantitative immunohistochemical survey of Cx43 Cx26 in 37 archival human cervical specimens. RESULTS: Cx43 expression was very low in normal cervix (100%), but was increased in low-grade squamous intraepithelial lesions (LSILs) (64%), primarily in a parabasal distribution...
February 2012: Analytical and Quantitative Cytology and Histology
Mohamed Ali Bedaiwy, Javier Noriega, Mahmoud Abdel Aleem, Sajal Gupta, Ahmed M AbulHassan, Jennifer Brainard, Alaa M Ismail, Tommaso Falcone
OBJECTIVE: To evaluate peritoneal fluid hemosiderin-laden macrophages (H-LMs) in patients with endometriosis compared to controls. STUDY DESIGN: Consecutive series of 46 patients during a year undergoing laparoscopy for benign gynecologic conditions were included. The presence of H-LMs in peritoneal fluid was evaluated. We compared clinical factors in patients with or without endometriosis in respect to H-LM status. To assess the potential of H-LMs to diagnose endometriosis, the sensitivity and specificity were calculated...
February 2012: Analytical and Quantitative Cytology and Histology
Maristella Bianconi, Mario Scartozzi, Luca Faloppi, Alessandro Bittoni, Elena Maccaroni, Riccardo Giampieri, Michela Del Prete, Luciano Burattini, Stefano Cascinu
For several decades, metastatic renal cell carcinoma (mRCC) was considered resistant to therapy and thus linked to poor prognosis. Recent years have seen a complete revolution of the therapeutic landscape, with the introduction of new target therapies that have radically changed treatment strategies in this setting. These new agents have proved to be effective if directed mainly against the angiogenic pathway. The vascular endothelial growth factor family is, in fact, the main target of a number of new drugs, such as monoclonal antibodies and different generations of tyrosine-kinase inhibitors that are currently approved or in different stages of clinical trial for the treatment of mRCC patients...
February 2012: Analytical and Quantitative Cytology and Histology
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