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Cell Biology and Toxicology

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https://www.readbyqxmd.com/read/28540451/exogenous-cgrp-upregulates-profibrogenic-growth-factors-through-pkc-jnk-signaling-pathway-in-kidney-proximal-tubular-cells
#1
Sang Pil Yoon, Jinu Kim
Kidney denervation prevents the development of tubulointerstitial fibrosis, but the neuropeptide calcitonin gene-related peptide (CGRP) in the denervated kidneys restores the fibrotic feature through the upregulation of profibrogenic growth factors. CGRP is involved in aggravation of inflammation by increasing the number of circulating cells and chemotactic factors. However, it is not clear how CGRP contributes to the upregulation of profibrogenic factors during fibrogenesis. In both human and pig kidney proximal tubular cell lines, administration of 1 nM CGRP significantly increased the levels of transforming growth factor-β1 (TGF-β1) production and connective tissue growth factor (CTGF) expression at 6 and 24 h after the administration...
May 24, 2017: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/28474249/t-cell-modulation-in-immunotherapy-for-hematological-malignancies
#2
EDITORIAL
Chen Lin, Shaohua Chen, Yangqiu Li
No abstract text is available yet for this article.
May 4, 2017: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/28470556/critical-roles-of-mucin-1-in-sensitivity-of-lung-cancer-cells-to-tumor-necrosis-factor-alpha-and-dexamethasone
#3
Menglin Xu, Xiangdong Wang
Lung cancer is the leading cause of death from cancer. Mucins are glycoproteins with high molecular weight, responsible for cell growth, differentiation, and signaling, and were proposed to be correlated with gene heterogeneity of lung cancer. Here, we report aberrant expression of mucin genes and tumor necrosis factor receptors in lung adenocarcinoma tissues compared with normal tissues in GEO datasets. Mucin-1 (MUC1) gene was selected and considered as the target gene; furthermore, the expression pattern of adenocarcinomic cells (A549, H1650, or H1299 cells) was validated under the stimulation with tumor necrosis factor-alpha (TNFα) or dexamethasone (DEX), separately...
May 3, 2017: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/28466226/analysis-of-dna-damage-response-to-ionizing-radiation-in-serum-shock-synchronized-human-fibroblasts
#4
Samantha Corrà, Riccardo Salvadori, Leonardo Bee, Vito Barbieri, Maddalena Mognato
Many aspects of cellular physiology, including cellular response to genotoxic stress, are related to the circadian rhythmicity induced by the molecular clock. The current study investigated if the cellular response to DNA damage is in relation to endogenous expression levels of the PER2 protein, a key component of the molecular regulatory system that confers rhythmicity in mammalian cells. Human normal fibroblasts (CCD-34Lu) were subjected to serum shock to induce circadian oscillations of the PER2 protein and then irradiated with γ- rays at times corresponding to the trough and peak expression of the PER2 protein...
May 3, 2017: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/28455626/influences-of-flavones-on-cell-viability-and-camp-dependent-steroidogenic-gene-regulation-in-ma-10-leydig-cells
#5
Michelle Cormier, Firas Ghouili, Pauline Roumaud, William Bauer, Mohamed Touaibia, Luc J Martin
Testicular Leydig cells are major contributors of androgen synthesis and secretion, which play an important role in testis development, normal masculinization, maintenance of spermatogenesis, and general male fertility. The rate-limiting step in testosterone biosynthesis involves the transfer of cholesterol to the mitochondrial inner membrane by the steroidogenic acute regulatory (Star) protein, a critical factor in steroid hormone biosynthesis. Once inside the mitochondria, cholesterol is metabolized by the steroidogenic enzyme Cyp11a1 to pregnenolone, which is further converted to testosterone by the action of other steroidogenic enzymes...
April 28, 2017: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/28382404/prediction-of-hepatotoxicity-for-drugs-using-human-pluripotent-stem-cell-derived-hepatocytes
#6
Jong Hyun Kim, Min Wang, Jaehun Lee, Han-Jin Park, Chungseong Han, Hee Su Hong, Jeong Seong Kim, Geun Ho An, Kijung Park, Hee-Kyung Park, Shi Feng Zhu, Xiao-Bo Sun, Jong-Hoon Kim, Dong-Hun Woo
Drug-induced liver toxicity is a main reason for withdrawals of new drugs in late clinical phases and post-launch of the drugs. Thus, hepatotoxicity screening of drug candidates in pre-clinical stage is important for reducing drug attrition rates during the clinical development process. Here, we show commercially available hepatocytes that could be used for early toxicity evaluation of drug candidates. From our hepatic differentiation technology, we obtained highly pure (≥98%) hepatocytes from human embryonic stem cells (hESCs) having mature phenotypes and similar gene expression profiles with those of primary human tissues...
April 5, 2017: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/28289984/in-silico-characterization-and-transcriptomic-analysis-of-nif-family-genes-from-anabaena-sp-pcc7120
#7
Shilpi Singh, Alok Kumar Shrivastava
In silico approaches in conjunction with morphology, nitrogenase activity, and qRT-PCR explore the impact of selected abiotic stressor such as arsenic, salt, cadmium, copper, and butachlor on nitrogen fixing (nif family) genes of diazotrophic cyanobacterium Anabaena sp. PCC7120. A total of 19 nif genes are present within the Anabaena genome that is involved in the process of nitrogen fixation. Docking studies revealed the interaction between these nif gene-encoded proteins and the selected abiotic stressors which were further validated through decreased heterocyst frequency, fragmentation of filaments, and downregulation of nitrogenase activity under these stresses indicating towards their toxic impact on nitrogen fixation potential of filamentous cyanobacterium Anabaena sp...
March 14, 2017: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/28285358/fluoroquinolones-and-propionic-acid-derivatives-induce-inflammatory-responses-in-vitro
#8
Akira Nakajima, Hiroki Sato, Shingo Oda, Tsuyoshi Yokoi
Fluoroquinolones and propionic acid derivatives are widely used antibacterials and non-steroidal anti-inflammatory drugs, respectively, which have been reported to frequently trigger drug hypersensitivity reactions. Such reactions are induced by inflammatory mediators such as cytokines and chemokines. The present study investigated whether levofloxacin, a fluoroquinolone, and loxoprofen, a propionic acid derivative, have the potential to induce immune-related gene expression in dendritic cell-like cell lines such as HL-60, K562, and THP-1, and immortalized keratinocytes such as HaCaT...
March 11, 2017: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/28281027/proteasome-inhibitors-against-amelanotic-melanoma
#9
Justyna Sidor-Kaczmarek, Mirosława Cichorek, Jan Henryk Spodnik, Sławomir Wójcik, Janusz Moryś
The incidence of malignant melanoma, the most aggressive skin cancer, is increasing constantly. Despite new targeted therapies, the prognosis for patients with metastatic disease remains poor. Thus, there is a need for new combinational treatments, and antineoplastic agents potentially valuable in this approach are inhibitors of the ubiquitin-proteasome system (UPS). In this work, we analyze the cytotoxicity mechanisms of proteasome inhibitors (MG-132, epoxomicin, and lactacystin) in a specific form of melanoma which does not synthesize melanin-the amelanotic melanoma (Ab cells)...
March 9, 2017: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/28275881/unraveling-the-role-of-membrane-microdomains-during-microbial-infections
#10
REVIEW
Prathyusha Bagam, Dhirendra P Singh, Maria Eugenia Inda, Sanjay Batra
Infectious diseases pose major socioeconomic and health-related threats to millions of people across the globe. Strategies to combat infectious diseases derive from our understanding of the complex interactions between the host and specific bacterial, viral, and fungal pathogens. Lipid rafts are membrane microdomains that play important role in life cycle of microbes. Interaction of microbial pathogens with host membrane rafts influences not only their initial colonization but also their spread and the induction of inflammation...
March 9, 2017: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/28265775/altered-microrna-expression-patterns-during-the-initiation-and-promotion-stages-of-neonatal-diethylstilbestrol-induced-dysplasia-neoplasia-in-the-hamster-mesocricetus-auratus-uterus
#11
Ramesh Padmanabhan, Isabel R Hendry, Jennifer R Knapp, Bin Shuai, William J Hendry
Treatment of Syrian hamsters on the day of birth with the prototypical endocrine disruptor and synthetic estrogen, diethylstilbestrol (DES), leads to 100% occurrence of uterine hyperplasia/dysplasia in adulthood, a large proportion of which progress to neoplasia (endometrial adenocarcinoma). Consistent with our prior gene expression analyses at the mRNA and protein levels, we now report (based on microarray, real-time polymerase chain reaction, and in situ hybridization analyses) that progression of the neonatal DES-induced dysplasia/neoplasia phenomenon in the hamster uterus also includes a spectrum of microRNA expression alterations (at both the whole-organ and cell-specific level) that differ during the initiation (upregulated miR-21, 200a, 200b, 200c, 29a, 29b, 429, 141; downregulated miR-181a) and promotion (downregulated miR-133a) stages of the phenomenon...
March 6, 2017: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/28251411/a-fish-intestinal-epithelial-barrier-model-established-from-the-rainbow-trout-oncorhynchus-mykiss-cell-line-rtgutgc
#12
Matteo Minghetti, Carolin Drieschner, Nadine Bramaz, Hannah Schug, Kristin Schirmer
The intestine of fish is a multifunctional organ: lined by only a single layer of specialized epithelial cells, it has various physiological roles including nutrient absorption and ion regulation. It moreover comprises an important barrier for environmental toxicants, including metals. Thus far, knowledge of the fish intestine is limited largely to in vivo or ex vivo investigations. Recently, however, the first fish intestinal cell line, RTgutGC, was established, originating from a rainbow trout (Oncorhynchus mykiss)...
March 1, 2017: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/28474250/single-cell-crispr-screening-in-drug-resistance
#13
EDITORIAL
William Wang, Xiangdong Wang
No abstract text is available yet for this article.
June 2017: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/28064403/knockdown-of-clusterin-alters-mitochondrial-dynamics-facilitates-necrosis-in-camptothecin-induced-cancer-stem-cells
#14
Parthasarathy Arumugam, Annie Samson, Jieun Ki, Joon Myong Song
The existence of a well-established drug resistance mechanism in cancer stem cells (CSC) complicates the cancer treatment. Clusterin (CLU) plays a key role in maintaining the integrity of endoplasmic reticulum (ER) during drug-induced stress. Hence, silencing the CLU could significantly reduce the inherent drug resistance mechanism of CSC. The combination of drug-induced cytotoxicity, as well as the suppression of drug resistance in CSC, could circumvent the recurrence capability of the tumor. In the present study, camptothecin (CPT)-induced apoptosis and necrosis in CSC with and without siCLU treatment were simultaneously measured using Qdot-based total internal reflection fluorescence microscope (TIRF)...
June 2017: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/28039590/ten-years-of-ipsc-clinical-potential-and-advances-in-vitro-hematopoietic-differentiation
#15
REVIEW
Bárbara Cristina Martins Fernandes Paes, Pablo Diego Moço, Cristiano Gonçalves Pereira, Geciane Silveira Porto, Elisa Maria de Sousa Russo, Luiza Cunha Junqueira Reis, Dimas Tadeu Covas, Virginia Picanço-Castro
Ten years have passed since the first publication announcing the generation of induced pluripotent stem cells (iPSCs). Issues related to ethics, immune rejection, and cell availability seemed to be solved following this breakthrough. The development of iPSC technology allows advances in in vitro cell differentiation for cell therapy purpose and other clinical applications. This review provides a perspective on the iPSC potential for cell therapies, particularly for hematological applications. We discuss the advances in in vitro hematopoietic differentiation, the possibilities to employ iPSC in hematology studies, and their potential clinical application in hematologic diseases...
June 2017: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/27987184/look-who-s-talking-the-crosstalk-between-oxidative-stress-and-autophagy-supports-exosomal-dependent-release-of-hcv-particles
#16
REVIEW
Regina Medvedev, Eberhard Hildt, Daniela Ploen
Autophagy is a highly conserved and regulated intracellular lysosomal degradation pathway that is essential for cell survival. Dysregulation has been linked to the development of various human diseases, including neurodegeneration and tumorigenesis, infection, and aging. Besides, many viruses hijack the autophagosomal pathway to support their life cycle. The hepatitis C virus (HCV), a major cause of chronic liver diseases worldwide, has been described to induce autophagy. The autophagosomal pathway can be further activated in response to elevated levels of reactive oxygen species (ROS)...
June 2017: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/27981389/pro-differentiating-effects-of-a-synthetic-flavagline-on-human-teratocarcinomal-cancer-stem-like-cells
#17
Fathi Emhemmed, Sarah Ali Azouaou, Qian Zhao, Aline Appert-Collin, Amar Bennasroune, Valérie B Schini-Kerth, Christian D Muller, Laurent Désaubry, Guy Fuhrmann
As initiators of the carcinogenic process, cancer stem cells (CSCs) are considered as new targets for anti-cancer therapies. However, these cells are hidden in the cancer bulk and remain relatively insensitive to chemotherapy, which targets their proliferative capacities. Alternatively, growing evidences have pointed out that a differentiation therapy could adversely affect these cells, which consequently should lose their self-renewal properties and become less aggressive. In order to evaluate the differentiation potential of an emerging class of anti-cancer drugs, we used the poorly differentiated teratocarcinomal cell as a model of Oct4-expressing CSC and determined the molecular mechanisms induced by the highly active flavagline FL3...
June 2017: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/27942899/screening-of-molecular-cell-targets-for-carcinogenic-heterocyclic-aromatic-amines-by-using-calux%C3%A2-reporter-gene-assays
#18
Pablo Steinberg, Peter A Behnisch, Harrie Besselink, Abraham A Brouwer
Heterocyclic aromatic amines (HCAs) are compounds formed when meat or fish are cooked at high temperatures for a long time or over an open fire. To determine which pathways of toxicity are activated by HCAs, nine out of the ten HCAs known to be carcinogenic in rodents (2-amino-9H-pyrido[2,3-b]indole (AαC), 2-aminodipyrido[1,2-a:3',2-d]imidazole (Glu-P-2), 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), 2-amino-3-methyl-9H-pyrido[2,3-b]indole (MeAαC), 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ), 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx), 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), and 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2)) were tested in the estrogen receptor α (ERα), androgen receptor (AR), glucocorticoid receptor (GR), peroxisome proliferator-activated receptor γ2 (PPARγ2), polycyclic aromatic hydrocarbons (PAH), Nrf2, and p53 CALUX® reporter gene assays...
June 2017: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/27822587/disruption-of-endolysosomal-trafficking-pathways-in-glioma-cells-by-methuosis-inducing-indole-based-chalcones
#19
Nneka E Mbah, Jean H Overmeyer, William A Maltese
Methuosis is a form of non-apoptotic cell death involving massive vacuolization of macropinosome-derived endocytic compartments, followed by a decline in metabolic activity and loss of membrane integrity. To explore the induction of methuosis as a potential therapeutic strategy for killing cancer cells, we have developed small molecules (indole-based chalcones) that trigger this form of cell death in glioblastoma and other cancer cell lines. Here, we report that in addition to causing fusion and expansion of macropinosome compartments, the lead compound, 3-(5-methoxy-2-methyl-1H-indol-3-yl)-1-(4-pyridinyl)-2-propen-1-one (MOMIPP), disrupts vesicular trafficking at the lysosomal nexus, manifested by impaired degradation of EGF and LDL receptors, defective processing of procathepsins, and accumulation of autophagosomes...
June 2017: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/27796700/benzophenone-3-increases-metastasis-potential-in-lung-cancer-cells-via-epithelial-to-mesenchymal-transition
#20
Preeyaporn Plaimee Phiboonchaiyanan, Kesarin Busaranon, Chuanpit Ninsontia, Pithi Chanvorachote
Exposure to compounds with cancer-potentiating effects can contribute to the progression of cancer. Herein we have discovered for the first time that benzophenone-3 (BP-3), a chemical used as sunscreen in various cosmetic products, enhances the ability of lung cancer cells to undergo metastasis. The exposure of the lung cancer cells to BP-3 at non-toxic concentrations significantly increased the number of anoikis resistant cells in a dose-dependent manner. Also, BP-3 increased the growth rate as well as the number of colonies accessed by anchorage-independent growth assay...
June 2017: Cell Biology and Toxicology
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