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Immunological Investigations

Zahra Alizadeh, Marzieh Mazinani, Masoud Houshmand, Leila Shakerian, Maryam Nourizadeh, Zahra Pourpak, Mohammad Reza Fazlollahi
BACKGROUND: Hyper IgM Syndrome (HIGM) is a rare primary immunodeficiency in which impairment of class switching recombination (CSR) and somatic hyper-mutation (SHM) leads to recurrent infections. OBJECTIVES: The aim of this study is to report the clinical and genetic features of six Iranian HIGM patients. METHODS: Six patients, who suspected to have HIGM based on two clinical findings, including recurrent infections and low levels of IgG and IgA and normal or elevated levels of IgM, were entered this study to undergo genetic studies...
August 6, 2018: Immunological Investigations
Karthi Sreedevi, Sabarinath P Subramanian, Geetha Mandagini, Padinjaradath S Appukuttan
Two heavily O-glycosylated proteins and albumin co-purified with anti-α-galactoside (anti-Gal), the chief xenograft-rejecting antibody and anti-β-glucan (ABG) antibody isolated from human plasma by affinity chromatography on respective ligand-bearing matrices. Both antibodies and O-glycoproteins co-purified with plasma albumin eluted from albumin-specific matrix. Using components of affinity-purified antibody samples separated by electrophoresis binding of either albumin or antibody to the affinity matrix of the other or binding of O-glycoprotein to either matrix was ruled out...
August 6, 2018: Immunological Investigations
Vahid Mohammadi Shahrokhi, Ali Ravari, Tayebeh Mirzaei, Mohammad Zare-Bidaki, Gholamreza Asadikaram, Mohammad Kazemi Arababadi
BACKGROUND: Aging and its complications such as Alzheimer's disease (AD) are associated with chronic low-grade inflammation entitled age-associated inflammation. However, the main mechanisms whichinduce age-associated inflammation in aging and AD are yet to beclarified. L-23/IL-17A axis plays important roles in the induction of inflammation and consequently autoimmune disease. This review evaluates the main roles played by IL-17A, IL-23, and IL-17A/IL-23 axis in the pathogenesis of age-associated inflammation in AD patients...
August 6, 2018: Immunological Investigations
Zahra Adnani Sadati, Hossein Motedayyen, Roya Sherkat, Vajihe Ostadi, Nahid Eskandari
BACKGROUND: Common Variable Immunodeficiency (CVID) is a primary immunodeficiency characterized by an immunologic deficiency in immunoglobulin production. Regulatory T cells (Tregs) play a key role in preventing the development allergic disorders. p-STAT5 is a known factor for the function and survival of Tregs. This study aimed to investigate the number of Tregs and their p-STAT5 expression in allergic and non-allergic CVID patients. METHODS: Peripheral blood mononuclear cells (PBMCs) were isolated from 10 healthy volunteers, 10 allergic patients, and 16 CVID patients (allergic and non-allergic) using Ficoll density centrifugation...
July 31, 2018: Immunological Investigations
Jian Wang, Wanju Sun, Allen Bond, Chengpin Xu, Kai Li, Donglin Ren, Xin Song, Fang Tan, Kun Jiao, Ming Ni, Jun Zhang, Yunpeng Bai, Mingfei Wang
BACKGROUND: Endplate inflammation remains a difficult disease to treat, in part due to its unclear pathology. Previous experiments showed that patients with idiopathic inflammation presented a systemic upregulation of Th17 cells. Here, we investigated how this change might affect the inflammatory environment in endplate inflammation. METHODS: Peripheral blood was obtained from patients and healthy controls, and Th17 cells were examined. RESULTS: Th17 cells significantly increased the differentiation of CD11c+ and DC-SIGN+ dendritic cells (DCs) from circulating monocytes in the absence of exogenous stimulation as well as in the presence of LPS stimulation...
July 17, 2018: Immunological Investigations
Abdelhafidh Hajjej, Wassim Y Almawi, Mouna Stayoussef, Antonio Arnaiz-Villena, Lasmar Hattab, Slama Hmida
Several studies confirmed the association of HLA-DRB1 and -DQB1 alleles with altered risk of type 1 diabetes (T1D). However, data from individual studies based on small sample sizes yielded often conflicting findings in African Arabs. This is a systematic review and meta-analysis aimed at comprehensively evaluating this association with T1D, using molecular HLA data. Relevant studies were identified through systemic search of Medline/PubMed, Cochrane, Science Direct, ResearchGate, and EMBASE databases. Statistical analysis was carried out using RevMan, and Comprehensive Meta-analysis programs...
July 13, 2018: Immunological Investigations
Ata Mahmoodpoor, Hadi Hamishehkar, Kamran Shadvar, Zohreh Ostadi, Sarvin Sanaie, Seied Hadi Saghaleini, Nader D Nader
OBJECTIVE: To modulate the inflammatory response in respiratory distress syndrome (ARDS) with selenium. BACKGROUND: Selenium replenishes the glutathione peroxidase proteins that are the first line of defense for an oxidative injury to the lungs. METHODS: Forty patients with ARDS were randomized into two groups: the SEL+ group being administered sodium selenite and the SEL- group receiving normal saline for 10 days. Blood samples were taken on Day-0, DAY-7, and Day-14 for assessment of IL-1 beta, IL-6, C-reactive protein, GPx-3, and selenium...
July 12, 2018: Immunological Investigations
Shamsolmoulouk Najafi, Mahsa Mohammadzadeh, Fateme Rajabi, Alireza Zare Bidoki, Hila Yousefi, Elham Farhadi, Nima Rezaei
BACKGROUND: Recurrent aphthous stomatitis (RAS) is a common oral condition with a major impact on the quality of life. The condition is thought to be due to the overexpression of T helper-1(Th1)-related cytokines. Since interleukin-4 (IL-4) and its receptor (IL-4Rα) are antagonistic to Th-1 pathways, polymorphisms in their genes may also be involved in the pathogenesis of aphthous stomatitis. METHODS: Sixty-four patients diagnosed with minor RAS and 141 (age- and sex-matched) healthy controls were assessed for 3 single-nucleotide polymorphisms (SNPs) within the promoter region of the IL-4 gene (-1098G/T, -590C/T, and -33C/T), and 1 SNP in IL-4Rα gene (+1902 A/G)...
July 9, 2018: Immunological Investigations
Qin Dong, Chen Cai, Feng Gao, Pei Chen, Weixin Gong, Meihua Shen
BACKGROUND: Despite years of research, the treatment of acute kidney injury (AKI) remains a significant challenge. Animal studies presented causal links between elevated regulatory T cell (Treg) response and better prognosis in AKI. Previous studies in mice and humans showed that TIM-3+ Treg cells were more potent than TIM-3- Treg cells. In this study, we investigated the role of TIM-3 in Treg in AKI patients. METHODS: Peripheral blood from AKI patients and healthy controls were gathered, and TIM-3+ Treg subset was examined...
July 9, 2018: Immunological Investigations
Maheshkumar Poomarimuthu, Sivakumar Elango, Pravin Raj Solomon, Sambath Soundrapandian, Jayalakshmi Mariakuttikan
BACKGROUND: IL-23/Th17 signaling pathway plays a crucial role in the cell-mediated immune response against bacterial infections and also in the pathogenesis of inflammatory and autoimmune diseases. Recent studies indicate that Th17 cell-associated cytokines are involved in the progression and maintenance of valvular lesions in rheumatic heart disease (RHD). Variants in the genes of cytokines that are potentially involved in Th17 response may influence interindividual differences in their expression levels, thereby contributing to the pathogenesis of immune-mediated diseases such as RHD...
July 9, 2018: Immunological Investigations
Luis M Amezcua-Guerra, Rafael Bojalil, Jessica Espinoza-Hernandez, María E Vega-Memije, Rosa M Lacy-Niebla, Fernanda Ortega-Springall, Jorge Ortega-Hernández, Fausto Sánchez-Muñoz, Rashidi Springall
Psoriasis is triggered by several stimuli that share a systemic production of interferon (IFN)-γ and other inflammatory mediators, which are key to regulate the production of matrix metalloproteinase (MMP)-9 and its inhibitor (TIMP)-1 by cells of monocytic lineage. This study evaluates the effect of the sera of 55 patients with psoriasis and 41 non-psoriatic individuals on the production of MMP-9 and TIMP-1 in cultured monocytes from a single healthy blood donor and in U937 cells. The effect of IFN-γ stimulation was also evaluated...
July 6, 2018: Immunological Investigations
Horea Vladi Matei, Mihaela Laura Vica, Costel Vasile Siserman
Infection with hepatitis B virus (HBV) is a major problem worldwide. The major histocompatibility complex plays an essential role in host immunity and can help eliminate the HBV of infected hepatocytes. Our study aimed to determine the role of certain human leukocyte antigen (HLA) class II molecules (i.e. HLA-DRB1 and HLA-DQB1) in the persistence or removal of HBV. Sixty patients confirmed to be HBV-positive via real-time polymerase chain reaction (PCR), i.e. people with chronic active hepatitis, were included in the study along with a control group of 100 healthy individuals without evidence of HBV infection...
July 6, 2018: Immunological Investigations
Hamideh Moravvej, Pardis-Sadat Tabatabaei-Panah, Reyhaneh Abgoon, Leyla Khaksar, Masumeh Sokhandan, Saba Tarshaei, Sayyed Mohammad Hossein Ghaderian, Ralf J Ludwig, Reza Akbarzadeh
Alopecia areata (AA) is characterized by a genetically complex inheritance. HLA frequencies, as well as the single nucleotide polymorphism (SNP) in PTPN22, CTLA4, and IL2RA genes, have been described to be associated with AA susceptibility. So far, no independent replication of these studies has been reported, and no data exist on a possible association between AA disease and these SNPs or influence of HLA frequencies in Iranian population. A possible association between HLA-DRB1*11 alleles as well as a single variation in PTPN22, CTLA4, and IL2RA genes and patchy AA disease have been investigated in a cohort from Iran...
July 6, 2018: Immunological Investigations
Xiuhua Yao, Jiarui Zhao, Qingfei Kong, Xiaoli Xie, Jinghua Wang, Bo Sun, Lixia Xu, Lili Mu, Hulun Li
Interleukin-9 (IL-9) is a multifunctional cytokine involved in protective immunity or immunopathology depending on the microenvironment and specific disease settings. Our early study determined that IL-9 and Th9 cells participate in and promote the progression of experimental autoimmune myasthenia gravis (EAMG). The data from this study showed that exogenous recombinant rat IL-9 (rrIL-9) acted as an IL-9 receptor antagonist, reduced the incidence of EAMG in rats, alleviated the severity of the disease, and reduced the anti-acetylcholine receptor (AChR) IgG antibody levels by altering the Th-subset distribution...
June 26, 2018: Immunological Investigations
Mojtaba Shohan, Milad Sabzevary-Ghahfarokhi, Nader Bagheri, Hedayatollah Shirzad, Ghorbanali Rahimian, Amin Soltani, Mahdi Ghatreh-Samani, Fatemeh Deris, Kamran Tahmasbi, Elahe Shahverdi, Fereshteh Fathollahi
BACKGROUND: Ulcerative colitis (UC) is a chronic inflammatory disorder of the large intestine histologically characterized by indistinct sustained inflammatory responses. Genetical susceptibility and environmental factors' effects play the roles in disease occurrence and it can be life threatening if remains untreated. It seems that intensification of inflammatory responses in this condition is not restricted to a specific cell line of T lymphocytes. Our aim was to determine the number of T helper 9 (Th9) cells in inflamed colonic biopsies of UC patients...
June 21, 2018: Immunological Investigations
Jan Spacek, Michal Vocka, Irena Netikova, Helena Skalova, Pavel Dundr, Bohuslav Konopasek, Eva Zavadova, Petruzelka Lubos
BACKGROUND: The objective of this study was to investigate serum levels of immunosuppressive cytokines TGF beta 1 and VEGF and count of immune cells in peripheral blood in stage II and III colorectal cancer patients. METHODS: Blood samples were collected from 22 colorectal patients and 25 healthy controls before the start of treatment. All patients were examined by a clinical immunologist to exclude patients with immune disorders and autoimmune diseases. TGF beta 1 and VEGF were measured by ELISA, and anti-tumor cellular immunity cells (CD4, CD8, B cells, NK cells) were measured by flow cytometry...
June 20, 2018: Immunological Investigations
Bo Zhang, Xiao-Ling Li, Cun-Rui Zhao, Chen-Liang Pan, Zheng Zhang
OBJECTIVE: The etiology of cardiovascular disease (CVD) is complex owing to the interactions of genetic variance with environmental factors. Inflammatory processes are now being increasingly implicated in the pathogenesis of CVD. This meta-analysis investigated the potential role of interleukin-6 (IL-6) as a risk factor for CVD development in healthy individuals. METHODS: Literature search was carried out in multiple electronic databases, and study selection followed a priori eligibility criteria...
June 6, 2018: Immunological Investigations
Mohamad Akbari, Vahideh Hassan-Zadeh
OBJECTIVE: Innate immune system dysregulation and chronic low-grade inflammation are associated with the pathogenesis of type 2 diabetes (T2D). The aim of this study was to investigate the effect of hyperglycemia on mRNA expression of four inflammatory genes in peripheral blood mononuclear cells (PBMCs) of pre-diabetic and diabetic individuals. METHODS: In a case-control study, quantitative real-time PCR was used to analyze changes in IL-1β, IL1R1, IL-6, and IL6ST gene expression in PBMCs of 30 T2D patients with high blood glucose levels, 24 diabetic and nondiabetic individuals with moderately high blood glucose levels and 30 controls with normal blood glucose levels...
May 31, 2018: Immunological Investigations
Kate Rittenhouse-Olson
No abstract text is available yet for this article.
August 2018: Immunological Investigations
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