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Immunological Investigations

Paraskevi Matsota, Georgia Kostopanagiotou, Konstantinos Kalimeris, Ageliki Pandazi, Antigone Kotsaki, Styliani Kontogiannopoulou, Evangelos J Giamarellos-Bourboulis
The effects of anesthetics on immune cell apoptosis and cytokine stimulation were studied in a prospective study. American Society of Anesthesiologists I/II patients underwent elective inguinal hernia repair or varicose veins stripping surgery and were randomized to either epidural anesthesia (n = 14) or general anesthesia with sevoflurane (n = 19) or propofol (n = 15). Blood was sampled before anesthesia induction (T0), at the end of surgery (T1), and 6 h later (T2). Apoptosis was determined by ANNEXIN-V staining of white blood cells; monocytes were isolated and stimulated for cytokine production...
February 7, 2018: Immunological Investigations
Daniel Lotanna Ajaghaku, Peter Achunike Akah, Emmanuel Emeka Ilodigwe, Sunday Odunke Nduka, Uduma Eke Osonwa, Festus Basden Chinedu Okoye
BACKGROUND: Millettia aboensis (Hook. F.) Baker (Fabaceae) is popular in ethnomedicine for its acclaimed efficacy in a number of disease conditions. This study evaluated the immunomodulatory effect of the leaf extract as a possible mechanism of its ethnomedicinal uses. METHODS: Humoral and cellular immune responses of Balb/c mice to tetanus toxoid and cyclophosphamide, respectively, were used to monitor immunomodulatory activities of the ethanol leaf extract and fractions of M...
February 6, 2018: Immunological Investigations
Gehan Abd-Elfatah Tawfeek, Suzan Alhassanin
BACKGROUND: Non-Hodgkin lymphoma (NHL) is a major cancer in Egypt and worldwide and has many risk factors including genes involved in the immune response. AIM: we investigated the HLA-G 14bp gene polymorphism as a risk factor for NHL and its clinic pathologic features. The study involved 150 patients with NHL and 100 healthy control. Full histories, clinical examination, C.T scan and laboratory investigations such as CBC, LDH, ?2microglobulin and HCV RNA by qualitative real time PCR were performed for all subjects...
February 1, 2018: Immunological Investigations
Shanshan Xu, Qiyuan Li, Jinzhun Wu, Guobing Chen, Bizhen Zhu, Weiyue Gu
BACKGROUND: Primary immunodeficiency diseases are a group of genetic disorders that lead to increased propensity to a variety of infections, sometimes with fatal outcomes. METHOD: In this study, whole-exome sequencing (WES) was used to identify mutations in two patients suspected of having primary immunodeficiency. Sanger sequencing was used to confirm the results in the patients and their family. RESULT: One patient was diagnosed as X-linked severe combined immunodeficiency (X-SCID) and another patient as X-linked chronic granulomatous disease (X-CGD) by WES...
February 1, 2018: Immunological Investigations
Zahra Mirfeizi, Samira Tabaei, Yalda Ravanshad, Kamila Hashemzadeh, Elahe Kharazmi, Hassan Mehrad-Majd
BACKGROUND: The vitamin D receptor (VDR) gene polymorphisms have been reported to be related to the development of Behcet's disease (BD). However, the results have been inconsistent among diverse populations. Therefore, this comprehensive meta-analysis has been designed to assess a more accurate association between VDR polymorphisms and BD susceptibility. METHODS: An electronic literature search was conducted to identify eligible studies. Pooled odds ratios (OR) with corresponding 95% confidence interval (CI) were calculated in different genetic models to assess this association...
February 1, 2018: Immunological Investigations
Guimin Zhang, Jinmei Zhang, Manbao Kuang, Peng Lin
OBJECTIVE: In this study, we first performed a meta-analysis to assess the role of single-nucleotide polymorphism (SNP) within tumor necrosis factor alpha (TNF alpha) gene and TNF alpha expression in the risk of nasal polyposis. METHODS: STATA 12.0 software was utilized to conduct the Mantel-Haenszel statistics, Cohen statistics, Begg's test, Egger's tests and sensitivity analysis. RESULTS: We systemically carried out the database retrieval and initially identified 486 articles...
February 1, 2018: Immunological Investigations
Zhe Fan, Xingxin Xu, Xiangming Qi, Yonggui Wu
OBJECTIVE: To investigate the effect of TGF-β activated kinase-1(TAK1) inhibitor 5Z-7-oxozeaenol on the interaction between macrophages and mesangial cells exposed to high glucose. METHODS: The macrophages and mesangial cells were cultured separately or co-cultured and divided into seven groups: inhibitor control group, mannitol control group, normal control group, high glucose group and inhibitor groups. The expression of p-TAK1, TAK1 binding protein (TAB1), transcription factor NF - κ B (NF-κB p65) of macrophages were analyzed by Western blotting...
January 26, 2018: Immunological Investigations
Agnieszka Magryś, Agnieszka Bogut, Michał Kiełbus, Alina Olender
The objective of this study was to analyze how Staphylococcus epidermidis SCV and WT strains manipulate the PI3K/Akt/mTOR signaling pathway. Six S. epidermidis strains with normal phenotype (WT) and six S. epidermidis strains with SCV phenotype were isolated in parallel from six patients with the prosthetic hip joint infections. THP-1 activated cells were incubated with or without PI3K inhibitor-wortmannin or with mTOR inhibitor-rapamycin. Next, macrophages were exposed to S. epidermidis WT and SCV strains...
January 16, 2018: Immunological Investigations
B R Sakr, M M Elfishawi, M H ElArousy, A K Hatw, A N AbdulKarim, A B Tammam, A N Kotp, M E Hamed, I E Genedy, E D El Desouky, Z O Nawito
OBJECTIVE: Demonstration of rheumatoid arthritis (RA) characteristics in a large cohort of Egyptian patients. METHODS: Retrospective analysis of data of 3219 RA patients attending the Rheumatology outpatient clinic, Kasr Alainy Hospital, Cairo University; from January 1995 till December 2015. RESULTS: Mean age at disease onset was 36.1 ±13.4 years; 2774 (84%) were females and mean disease duration was 12.9 ±7.9 years. Regarding number of joint affected at disease onset; polyarticular pattern was found in 77...
January 16, 2018: Immunological Investigations
Rania M Gamal, Walid M Gamal, Abeer M Ghandour, Hanan Sayed M Abozaid, Mona Embarek Mohamed, Yasser Emad, Ahmed Abdel Galeel
OBJECTIVE: we aimed to study systemic sclerosis patients in order to assess osteoprotegerin/Receptor activator of nuclear factor-kB ligand (OPG/RANKL) system and find the relation of these biomarkers with the clinical features of the disease, the carotid intima thickness, markers of inflammation, lipid profile, and other laboratory characteristics. METHODS: both the level of (RANKL), (OPG) in sera of participants, in 30 (SSc) patients and the atherosclerotic changes affecting the common carotid artery were measured and, were compared to 30 healthy controls matched for age and sex...
January 16, 2018: Immunological Investigations
Youssef M Mosaad, Ehab Es El-Toraby, Ziyad Me Tawhid, Adel I Abdelsalam, Asmaa F Enin, Amany Me Hasson, Ghada M Shafeek
OBJECTIVE: To study the relation between CD266 rs763361 gene polymorphism and CD226 serum level and to evaluate their role in susceptibility and disease activity of RA in a cohort of Egyptian individuals. METHODS: The serum level of CD226 was measured using a suitable ELISA kit and the CD226 rs763361 gene polymorphism was typed by PCR-RFLP for 112 RA patients and 100 healthy controls. RESULTS: Significant association with RA was found with CD226 T allele (OR (95%CI) = 1...
January 10, 2018: Immunological Investigations
Mario Štefanić, Stana Tokić, Mirjana Suver Stević, Ljubica Glavaš-Obrovac
PURPOSE: Studies of cytotoxic T cells and their respective lineage master regulators have been limited in Hashimoto's thyroiditis (HT). It is unclear whether their transcriptomes are changed in HT patients and how these changes are associated with the thyroid damage, major clinical manifestations, and disease progression. METHODS: We explored the gene expression patterns of selected transcription factors [eomesodermin (EOMES), BACH2, BCL6, TCF1] and cytolytic molecules [granzyme B (GZMB)] in peripheral blood (PB) T cells of 10 healthy controls and 30 HT patients of various subtypes (hypothyroid, untreated HT; L-thyroxine (T4)-treated HT, and spontaneously euthyroid HT) using real-time quantitative PCR...
January 10, 2018: Immunological Investigations
Lin-Nan Shao, Yang Yang, Shu-Ting Zhang, Shi-Hang Zhou, Ying Duan, Qing-Li Meng, Xiao-Hua Liang, Wei-Jian Yu
BACKGROUND: End-stage renal disease (ESRD), the last stage of chronic renal failure, is a global health problem. The number of ESRD patients worldwide is increasing faster than the number of kidneys available per year for renal transplantation. Most of the ESRD patients are awaiting renal transplantation. The immune response to the transplanted kidney is directed mainly against mismatched human leukocyte antigen (HLA) glycoproteins expressed on donor tissues. Thus, the analysis of HLA allele and haplotype polymorphisms is valuable not only for identifying ESRD susceptibility factors but also to improve graft survival...
December 19, 2017: Immunological Investigations
Gamal Allam, Amre Nasr, Iman M Talaat, Abdelaziz S A Abuelsaad, Ali M Bakheit, Dalal Nemenqani, Adnan A Alsulaimani
BACKGROUND: Association studies of genes encoding cytokines that play an important role in inflammatory response represent one approach to finding type 1 diabetes (T1D) disease genes. The aim of this study was to investigate the association of single nucleotide polymorphisms (SNPs) within cytokine genes with T1D in a cohort of Saudi subjects. METHODS: A total of 300 well-characterized type 1 diabetic patients and 300 T1D-free control subjects were enrolled in this investigation...
December 19, 2017: Immunological Investigations
E Preciado-Martínez, G García-Ruíz, P Flores-Espinosa, L Bermejo-Martínez, A Espejel-Nuñez, G Estrada-Gutiérrez, G Razo-Aguilera, M Granados-Cepeda, A C Helguera-Repetto, C Irles, V Zaga-Clavellina
Progesterone is an essential hormone that induces deep immune adaptations favoring pregnancy maintenance. We aimed at evaluating the effects of progesterone on the synthesis of pro- and anti-inflammatory cytokines by mononuclear cells isolated from human placental blood stimulated with lipopolysaccharide, emulating an infection-inflammation environment. Mononuclear cells isolated form human placental blood were obtained from nine women undergoing elective cesarean delivery at term (not in labor), isolated by density gradient sedimentation, cultured and co-stimulated with lipopolysaccharide (500 ng/ml) from Escherichia coli in the presence or not of progesterone (0...
December 13, 2017: Immunological Investigations
Valle-Jiménez Xareni Raque, Sánchez-García Juan Carlos, Revilla-Rodríguez Eduardo, Baltierrez-Hoyos Rafael, Romero-Tlalolini María de Los Ángeles, Ramírez-Cosmes Adriana, Torres-Aguilar Honorio, Bustos-Arriaga José, Aguilar-Ruiz Sergio Roberto
Sepsis is an organic dysfunction that puts at risk the life of patients suffering this disorder due to an exacerbated immunological response to the infection mediated by the host. Platelets have been largely researched on sepsis owing to its role in Disseminated Intracellular Coagulation (DIC) and because thrombocytopenia is an important clinical feature of these patients. Nevertheless, a great number of evidence shows that platelets have also an important role in immunological response since they have pattern recognition receptors, chemokine receptors and granules with stored soluble mediators...
December 8, 2017: Immunological Investigations
Serena Bonin, Nunzia Zanotta, Arianna Sartori, Alessio Bratina, Paolo Manganotti, Giusto Trevisan, Manola Comar
BACKGROUND: Cerebrospinal fluid (CSF) analysis in patients with particular neurologic disorders is a powerful tool to evaluate specific central nervous system inflammatory markers for diagnostic needs, because CSF represents the specific immune micro-environment to the central nervous system. METHODS: CSF samples from 49 patients with multiple sclerosis (MS), chronic inflammatory demyelinating polyneuropathy (CIDP), and non-inflammatory neurologic disorders (NIND) as controls were submitted to protein expression profiles of 47 inflammatory biomarkers by multiplex Luminex bead assay to investigate possible differences in the inflammatory process for MS and CIDP...
November 28, 2017: Immunological Investigations
Nadia Elwan, Mohamed Labib Salem, Abdelrahman Kobtan, Ferial El-Kalla, Loai Mansour, Mohamed Yousef, Ashraf Al-Sabbagh, Abdel-Aziz A Zidan, Sherief Abd-Elsalam
BACKGROUND: Hepatocellular carcinoma (HCC) is the 3rd most common cause of cancer-related death worldwide. It has evolved different immune escape mechanisms, which might include emergence of lymphoid and myeloid regulatory cells. Aim of this work: To determine the numbers of Myeloid-derived suppressor cells (MDSCs) in peripheral blood and ascitic fluid in cirrhosis and HCC and their relation to IFN-γ and α-fetoprotein (α-FP). PATIENTS AND METHODS: Sixty individuals were enrolled in this study; forty cirrhotic patients with ascites; twenty without HCC (Group I), and twenty with HCC (group II) as well as twenty healthy individuals as a control group (group III)...
November 28, 2017: Immunological Investigations
Fanny Poujol, Guillaume Monneret, Emmanuelle Gallet-Gorius, Alexandre Pachot, Julien Textoris, Fabienne Venet
Profound T-cell alterations are observed in septic patients in association with increased risk of secondary infection and mortality. The pathophysiological mechanisms leading to such dysfunctions are not completely understood and direct and indirect mechanisms have been described. In this study we evaluated whether ex vivo stimulation of lymphocytes with IL-10, an immunosuppressive cytokine released at the systemic level during sepsis, could mimic sepsis-induced intrinsic T-cell alterations. We showed that recombinant human IL-10 priming of T cells altered their proliferative response to anti-CD2/CD3/CD28 antibody-coated beads and PHA stimulations, in a dose-dependent manner independently of accessory cells...
November 28, 2017: Immunological Investigations
Si Li, Qin Zhang, Peng Wang, Jun Li, Jing Ni, Jun Wu, Yan Liang, Rui-Xue Leng, Hai-Feng Pan, Dong-Qing Ye
OBJECTIVE: This study was performed to systematically summarize the results on the association of HLA-DQB1 polymorphisms with pemphigus vulgaris (PV) and other related factors. METHODS: A comprehensive literature search of PubMed, The Cochrane Library, Embase, and Google Scholar database was conducted to identify relevant articles in English, with the last report up to November 1, 2016. Heterogeneity test was performed, and publication bias was evaluated. Stata software 12...
November 28, 2017: Immunological Investigations
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