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Journal of Basic Microbiology

Li Zhu, Xiuping Qian, Daijie Chen, Mei Ge
Analysis of the Amycolatopsis orientalis genome revealed that two genes, hemA1 and hemA2, belonging to divergent pathways, were involved in the biosynthesis of 5-aminolevulinic acid. The roles of hemA1 and hemA2 were elucidated via genetic manipulation and metabolite analysis. The disruption of hemA1, encoding the glutamyl-tRNA(Glu) reductase of the C5 pathway, was essential for cell growth and is used for heme synthesis. Overexpression of hemA1 resulted in elevated vancomycin and ECO-0501 production in Amycolatopsis orientalis, and it was also effective in increasing the production of daptomycin and natamycin in other Streptomycetes...
November 22, 2017: Journal of Basic Microbiology
Xiu-Xiu Ren, Yuchen Wang, YongXiang Liu, Yumei Tan, Chunguang Ren, Yongyi Ge, Zuoyi Liu
Aspergillus cristatus develops into various stages under different Na concentrations: the sexual stage in 0.5 M NaCl and asexual development stage in 3 M NaCl. In order to explore whether the Ca(2+) signaling pathway in A. cristatus responded to the changes in the salt stress, we analyzed the gene expression levels in A. cristatus respectively cultured in 0.5 M NaCl and 3 M NaCl. According to the BLAST analysis results, we identified 25 Ca(2+) -signaling proteins in A. cristatus. The expression levels of most genes involved in the Ca(2+) -signaling pathway in A...
November 20, 2017: Journal of Basic Microbiology
Geeta G Shirnalli, Manish S Kaushik, Ajay Kumar, Gerard Abraham, Pawan K Singh
Cyanobacteria are known to exhibit their efficiency in producing high concentrations of compounds of commercial value. Arthrospira is one such cyanobacterium which is considered as important source of protein (65%) and other nutrients. In present study, chemical mutagenesis using N-methyl-Ń-Nitro-nitrosoguanidine (NTG), a proven potent mutagen for cyanobacteria was used to bring stable and desirable alteration in Arthrospira platensis ARM 730. Three morphological mutants (G-1, G-2, and SF) were selected and characterized...
November 17, 2017: Journal of Basic Microbiology
Indrani Sarkar, Louis S Tisa, Maher Gtari, Arnab Sen
Amino acid and protein biosynthesis requires a number of high energy phosphate bonds and includes a dual energy cost for the synthesis of chemical intermediates during the fueling reactions and the conversion of precursor molecules to final products. One popular hypothesis is that the proteins encoded by putative highly expressed genes (hence called PHXPs) generally utilize low energy consuming amino acids to reduce the biosynthetic cost of the essential proteins. In our study, we found that this idea was not supported in the case of actinobacteria...
November 16, 2017: Journal of Basic Microbiology
Isabella Grishkan
Composition of thermotolerant mycobiota in the soil of Israeli deserts and northern territories was examined in spatiotemporal dynamics. A total of 165 species from 82 genera were isolated at 37 °C using the soil dilution plate method. Aspergilli (Aspergillus fumigatus and A. niger) and teleomorphic ascomycetes (Canariomyces notabilis, Chaetomium nigricolor, and Ch. strumarium) comprised the basic part of the thermotolerant communities. The desert communities remarkably differed from the northern communities by a much higher abundance of A...
November 16, 2017: Journal of Basic Microbiology
Ashraf M M Essa, Mohamed A Al Abboud, Sayeed I Khatib
Microorganisms can modify the chemical and physical characters of metals leading to an alteration in their speciation, mobility, and toxicity. Aqueous heavy metals solutions (Hg, Cd, Pb, Ag, Cu, and Zn) were treated with the volatile metabolic products (VMPs) of Escherichia coli Z3 for 24 h using aerobic bioreactor. The effect of the metals treated with VMPs in comparison to the untreated metals on the growth of E. coli S1 and Staphylococcus aureus S2 (local isolates) was examined. Moreover, the toxic properties of the treated and untreated metals were monitored using minimum inhibitory concentration assay...
November 15, 2017: Journal of Basic Microbiology
Ahmed Anan, Khaled M Ghanem, Amira M Embaby, Ahmed Hussein, Moustafa Y El-Naggar
The present study underlines a unique promising approach toward efficient biotransformation of ceftriaxone sodium (Ceftx), a highly frequent prescribed cephalosporin antibiotic, by a newly bacterium namely Achromobacter xylosoxidans strain Cef6 isolated from Ceftx contaminated raw materials in pharmaceutical industries. A three step sequential statistical-mathematical approach (Plackett-Burman design [PBD], Central Composite Design [CCD], and ridge-canonical analyses) was anticipated to optimize the biotransformation process...
November 15, 2017: Journal of Basic Microbiology
Farakh Javed, Khanzadi N Manzoor, Mubashar Ali, Irshad U Haq, Abid A Khan, Assad Zaib, Sobia Manzoor
Zika virus is one of the emerging viruses and is of significant threat to human health globally. It is a mosquito borne flavivirus similar to dengue, yellow fever, and West Nile viruses. It was reported about 5 decades ago and then it spreads to different parts of the world. Large outbreaks were reported on Yap Islands in 2007. Now it has gained wide attention globally by health communities. Major vector for virus transmission is Aedes aegypti mosquito. ZIKV infection is mostly asymptomatic but it is also responsible to cause mild influenza like illness to serious manifestations...
November 13, 2017: Journal of Basic Microbiology
Tariqul Islam, Changsheng Peng, Imran Ali
Magnetotactic bacteria (MTB) are getting much attention in the recent years due to the biomineralization in their magnetosomes (MS). MS are unique organelles that are bio-mineralized due to MTB. MS contains nanosized crystal minerals of magnetite or greigite covered by bilayer lipid membrane, which are originated from cytoplasmic membrane (CM). MS are organized as an ordered chain into the cell which acts as a miniature compass needle. Furthermore, the biodiversity of MTB and their distribution is principally linked with the characteristics and growths of the MS...
November 7, 2017: Journal of Basic Microbiology
Shuangxin Ma, Ning Liu, Hui Jia, Dongqing Dai, Jinping Zang, Zhiyan Cao, Jingao Dong
Laccases are multicopper oxidases (E.C. that catalyze the oxidation of many phenolic compounds. In this study, a novel laccase, Stlac4, from Setosphaeria turcica was cloned and expressed in Escherichia coli by insertion into the pET-30a expression plasmid. The recombinant laccase was purified and visualized on SDS-PAGE as a single band with an apparent molecular weight of 71.5 KDa, and confirmed by Western blot. The maximum activity of the purified laccase was 127.78 U · mg(-1) , the optimum temperature and pH value were 60 °C and 4...
November 7, 2017: Journal of Basic Microbiology
Gints Kalnins, Eduards Sevostjanovs, Dace Hartmane, Solveiga Grinberga, Kaspars Tars
CntA oxygenase is a Rieske 2S-2Fe cluster-containing protein that has been previously described as able to produce trimethylamine (TMA) from carnitine, gamma-butyrobetaine, glycine betaine, and in one case, choline. TMA found in humans is exclusively of bacterial origin, and its metabolite, trimethylamine oxide (TMAO), has been associated with atherosclerosis and heart and renal failure. We isolated four different Rieske oxygenases and determined that there are no significant differences in their substrate panels...
November 7, 2017: Journal of Basic Microbiology
Satbir Singh, Puneet Gupta, Bijender K Bajaj
Process apt microbial proteases due to their wide range industrial applications have become the focus of intense scientific research during recent years. Considering the hostile process milieu, the proteases intended for application must be robust enough to withstand the extremes of temperature and pH, and presence of organic solvents and other potential enzyme inhibitors. Current study presents the characterization of a robust protease from a previously isolated bacterium Bacillus subtilis K-1 (BSK-1). Purification of BSK-1 protease (5...
November 6, 2017: Journal of Basic Microbiology
Zongling Liu, Shuang Du, Yi Ren, Yanlin Liu
A total of 216 killer yeasts Saccharomyces cerevisiae, isolated from wine, were evaluated in controlling Colletotrichum gloeosporioides, a pre-harvest anthracnose agent of grape. Three of these yeast isolates were tested positive for antagonizing C. gloeosporioides and were further evaluated for their mechanisms as biological control agents (BCAs): production of antifungal compounds, production of hydrolytic enzymes, inhibition of C. gloeosporioides conidia germination, colonization on grape berry, and efficiency in controlling anthracnose of grape...
November 6, 2017: Journal of Basic Microbiology
Alireza Jafari, Saeedeh Jafari Nodooshan, Roya Safarkar, Farahnaz Movahedzadeh, Nader Mosavari, Aren Novin Kashani, Mahdi Dehghanpour, Morteza Kamalzadeh, Samira Rasouli Koohi, Sara Fathizadeh, Ali Majidpour
The World Health Organization acknowledges tuberculosis as a global threat. Tuberculosis infection is one of the top 10 causes of death worldwide. Nanotechnology and microbiology researchers are looking for new and safe nano drugs for eliminating Mycobacterium tuberculosis, the causative agent of tuberculosis. In this study, AgZnO nano-crystals (AgZnONCs) is synthesized via the decomposition of the precursor of oxalate method. Characterization of AgZnONCs were evaluated. Next, various concentrations of AgZnONCs, as well AgZnONCs+Rifampicin, were prepared...
November 6, 2017: Journal of Basic Microbiology
Folguni Laskar, Sumi Das Purkayastha, Aniruddha Sen, Mrinal K Bhattacharya, Biswapriya B Misra
About half of the global methane (CH4 ) emission is contributed by the methanogenic archaeal communities leading to a significant increase in global warming. This unprecedented situation has increased the ever growing necessity of evaluating the control measures for limiting CH4 emission to the atmosphere. Unfortunately, research endeavors on the diversity and functional interactions of methanogens are not extensive till date. We anticipate that the study of the diversity of methanogenic community is paramount for understanding the metabolic processes in freshwater lake ecosystems...
October 30, 2017: Journal of Basic Microbiology
Vladimir Gorshkov, Nadezhda Tarasova, Natalia Gogoleva, Elena Osipova, Olga Petrova, Evgeny Kovtunov, Yuri Gogolev
In the present study, we attempted to elucidate if the harmful phytopathogenic bacteria of Pectobacterium genus (P. atrosepticum) possess the enzymes for oxidation of phenolic compounds. Polyphenol oxidase (laccase) activity was revealed in P. atrosepticum cell lysates. Using bioinformatic analysis, an ORF encoding a putative copper-containing polyphenol oxidase of 241 amino acids with a predicted molecular mass of 25.9 kDa was found. This protein (named Pal1) shares significant level of identity with laccases of a new type described for several bacterial species...
October 25, 2017: Journal of Basic Microbiology
Zheng-Bing Guan, Kai-Qiang Wang, Yan Shui, Xiang-Ru Liao
In this study, we established a Cre/loxP mutant recombination system (Cre/lox71-66 system) for markerless gene deletion to facilitate our follow-up rational genetic engineering to the strain Bacillus pumilus W3. This modified method uses two mutant loxP sites, which after recombination creates a double-mutant loxP site that is poorly recognized by Cre recombinase, facilitating multiple gene deletions in a single genetic background. Two selected genes, cotA and sigF, were continuously knocked out and verified at different levels using this method...
October 20, 2017: Journal of Basic Microbiology
Chekidhenkuzhiyil Jasmin, Abdulaziz Anas, Balu Tharakan, Abdul Jaleel, Vipindas Puthiyaveettil, Saravanane Narayanane, Jovitha Lincy, Shanta Nair
We examined the diversity of Planctomycetes in the sediment sample collected from an oxygen minimum zone (OMZ) in the southeast Arabian Sea. A 16SrRNA gene library was constructed using the forward primer specific for Planctomycetes and a universal reverse primer. The 237 sequences obtained were grouped into 130 operational taxonomic units, and the majority of them were clustered with phylum Planctomycetes (45.0%) and unclassified bacteria (27.0%). There were sequences that clustered with distantly separated monophyletic groups such as Latescibacteria (9%), Actinobacteria (6%), Proteobacteria (5%), and others (8%)...
September 26, 2017: Journal of Basic Microbiology
Shiling Zheng, Bingchen Wang, Ying Li, Fanghua Liu, Oumei Wang
Iron (III)-reducing bacteria (IRB) play significant roles in the degradation of naturally occurring organic matter and in the cycling of heavy metals in marine and freshwater sediments. Our previous study has demonstrated the co-occurrence of Geobacteraceae and Methanosarcinamazei as aggregates in the iron (III)-reducing enrichments from a coastal gold mining site on the Jiehe River. The IRB community in the enriched sediments was dominated by members of Comamonadacea, Clostridiaceae, Bacillaceae, Bacteroidaceae, and Geobacteraceae...
September 26, 2017: Journal of Basic Microbiology
María Z Simon Sola, Daiana Pérez Visñuk, Claudia S Benimeli, Marta Alejandra Polti, Analia Alvarez
Environmental mixed pollution by both organic and inorganic compounds are detected worldwide. Phytoremediation techniques have been proposed as ecofriendly methods for cleaning up polluted sites. Several studies have demonstrated enhanced dissipation of contaminants at the root-soil interface through an increase in microbial activity caused by the release of plant root exudates (REs). The aim of this study was to evaluate the effectiveness for Cr(VI) and lindane removal by Streptomyces M7 cultured in a co-contaminated system in presence of maize REs...
September 21, 2017: Journal of Basic Microbiology
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