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Journal of Basic Microbiology

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https://www.readbyqxmd.com/read/28543056/cloning-and-analysis-of-the-glwc-1-and-glwc-2-genes-encoding-putative-blue-light-photoreceptor-from-ganoderma-lucidum
#1
Xinran Xu, Xiangdong Chen, Wumengxiao Yu, Yu Liu, Weiwei Zhang, Jin Lan
Blue light plays an important role during the growth of Ganoderma lucidum, one of the best-known medicinal macrofungi in China. In the present study, we cloned Glwc-1 and Glwc-2, the homologue of the blue light photoreceptors Ncwc-1 and Ncwc-2 of Neurospora crassa, from G. lucidum. The deduced amino acid sequence of Glwc-1 contained the similar function domains as NcWC-1 including LOV, PAS B, PAS C, and PAC domains. The deduced amino acid sequence of Glwc-2 contained PAS domain and GATA-type zinc finger (Znf) domain as well as NcWC-2...
May 23, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28543691/emergent-macrophytes-modify-the-abundance-and-community-composition-of-ammonia-oxidizers-in-their-rhizosphere-sediments
#2
Dayong Zhao, Xiaowei He, Rui Huang, Wenming Yan, Zhongbo Yu
Ammonia oxidation is a crucial process in global nitrogen cycling, which is catalyzed by the ammonia oxidizers. Emergent plants play important roles in the freshwater ecosystem. Therefore, it is meaningful to investigate the effects of emergent macrophytes on the abundance and community composition of ammonia oxidizers. In the present study, two commonly found emergent macrophytes (Zizania caduciflora and Phragmitas communis) were obtained from freshwater lakes and the abundance and community composition of the ammonia-oxidizing prokaryotes in the rhizosphere sediments of these emergent macrophytes were investigated...
May 22, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28543603/silver-nanoparticles-antimicrobial-activity-cytotoxicity-and-synergism-with-n-acetyl-cysteine
#3
Selwan Hamed, Mohamed Emara, Riham M Shawky, Ramadan A El-Domany, Tareq Youssef
The fast progression of nanotechnology has led to novel therapeutic interventions. Antimicrobial activities of silver nanoparticles (Ag NPs) were tested against standard ATCC strains of Staphylococcus aureus (ATCC 9144), Escherichia coli (O157:H7), Pseudomonas aeruginosa (ATCC 27853), and Candida albicans (ATCC 90028) in addition to 60 clinical isolates collected from cancer patients. Antimicrobial activity was tested by disk diffusion method and MIC values for Ag NPs alone and in combination with N-acetylcysteine (NAC) against tested pathogens were determined by broth microdilution method...
May 22, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28543536/cyanobacteria-as-efficient-producers-of-mycosporine-like-amino-acids
#4
REVIEW
Shikha Jain, Ganshyam Prajapat, Mustari Abrar, Lalita Ledwani, Anoop Singh, Akhil Agrawal
Mycosporine-like amino acids are the most common group of transparent ultraviolet radiation absorbing intracellular secondary metabolites. These molecules absorb light in the range of ultraviolet-A and -B with a maximum absorbance between 310 and 362 nm. Cyanobacteria might have faced the most deleterious ultraviolet radiation, which leads to an evolution of ultraviolet protecting mycosporine-like amino acids for efficient selection in the environment. In the last 30 years, scientists have investigated various cyanobacteria for novel mycosporine-like amino acids, applying different induction techniques...
May 22, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28543439/metagenomic-profiling-of-the-microbial-freshwater-communities-in-two-bulgarian-reservoirs
#5
Ivan Iliev, Galina Yahubyan, Mariana Marhova, Elena Apostolova, Mariyana Gozmanova, Gana Gecheva, Sonya Kostadinova, Angelina Ivanova, Vesselin Baev
Microorganisms inhabiting freshwater environments are an integral part of the aquatic ecosystems. Very few data are available regarding the profiles of the microbial communities in the reservoirs in Bulgaria, despite their key role in the biogeochemical processes. In the present study, we provide the first comprehensive metagenomic analysis on the planktonic bacterial diversity of two large and economically important Bulgarian reservoirs (Batak and Tsankov Kamak) using next-generation sequencing of 16S ribosomal RNA gene (16S rRNA)...
May 22, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28513878/gene-cloning-expression-and-characterization-of-trehalose-6-phosphate-synthase-from-pleurotus-ostreatus
#6
Min Lei, Xiangli Wu, Jinxia Zhang, Hexiang Wang, Chenyang Huang
Trehalose-6-phosphate synthase (TPS; EC2.4.1.15) catalyzes the first step in trehalose synthesis, which involves transfer of glucose from uridine diphosphate glucose (UDPG) to glucose 6-phosphate (G6P) to form trehalose-6-phosphate. To determine the gene and enzymatic characteristics of TPS in Pleurotus ostreatus, we cloned and sequenced the cDNA of PoTPS1, which contains a 1665 bp open reading frame that encodes a 554-amino acid protein with a predicted molecular weight of 62.01 kDa. This gene was expressed in Escherichia coli BL21 and then the recombinant protein was purified and characterized...
May 17, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28503798/neutral-and-alkaline-cellulases-production-engineering-and-applications
#7
REVIEW
Ines Ben Hmad, Ali Gargouri
Neutral and alkaline cellulases from microorganisms constitute a major group of the industrial enzymes and find applications in various industries. Screening is the important ways to get novel cellulases. Most fungal cellulases have acidic pH optima, except some fungi like Humicola insolens species. However, new applications require the use of neutral and alkaline cellulases in food, brewery and wine, animal feed, textile and laundry, pulp and paper industries, agriculture as well in scientific research purposes...
May 15, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28493363/salinity-fluctuation-influencing-biological-adaptation-growth-dynamics-and-na-k-atpase-activity-in-a-euryhaline-bacterium
#8
Hao Yang, Yang Meng, Youxin Song, Yalin Tan, Alan Warren, Jiqiu Li, Xiaofeng Lin
Although salinity fluctuation is a prominent characteristic of many coastal ecosystems, its effects on biological adaptation have not yet been fully recognized. To test the salinity fluctuations on biological adaptation, population growth dynamics and Na(+) /K(+) -ATPase activity were investigated in the euryhaline bacterium Idiomarina sp. DYB, which was acclimated at different salinity exposure levels, exposure times, and shifts in direction of salinity. Results showed: (1) bacterial population growth dynamics and Na(+) /K(+) -ATPase activity changed significantly in response to salinity fluctuation; (2) patterns of variation in bacterial growth dynamics were related to exposure times, levels of salinity, and shifts in direction of salinity change; (3) significant tradeoffs were detected between growth rate (r) and carrying capacity (K) on the one hand, and Na(+) /K(+) -ATPase activity on the other; and (4) beneficial acclimation was confirmed in Idiomarina sp...
May 11, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28485524/streptococcus-gordonii-luxs-autoinducer-2-quorum-sensing-system-modulates-the-dual-species-biofilm-formation-with-streptococcus-mutans
#9
Xiao Wang, Xiaolan Li, Junqi Ling
Dental plaques are mixed-species biofilms that are related to the development of dental caries. Streptococcus mutans (S. mutans) is an important cariogenic bacterium that forms mixed-species biofilms with Streptococcus gordonii (S. gordonii), an early colonizer of the tooth surface. The LuxS/autoinducer-2(AI-2) quorum sensing system is involved in the regulation of mixed-species biofilms, and AI-2 is proposed as a universal signal for the interaction between bacterial species. In this work, a S. gordonii luxS deficient strain was constructed to investigate the effect of the S...
May 9, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28485502/indole-enhances-the-survival-of-pantoea-ananatis-yj76-in-face-of-starvation-conditions
#10
Jing Zheng, Jiajia Yu, Mengqi Jia, Liping Zheng, Yongjun Feng
Pantoea ananatis YJ76 is an indole-producing predominant diazotrophic endophyte isolated from rice having multiple growth-promoting effects on host plant. As a decomposition metabolite of L-tryptophan (L-Trp), indole is confirmed to regulate various physiological processes of bacteria. In this research, we found that indole significantly improves the survival of YJ76 in face of starvation conditions and the promoting effect is related to the glycogen accumulation promoted by indole, which is much more significant in the middle decline phase than in other growth phases...
May 9, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28471518/bacterial-microbiota-in-small-brown-planthopper-populations-with-different-rice-viruses
#11
Shuo Li, Changwei Zhou, Guangyi Chen, Yijun Zhou
The small brown planthopper (SBPH) is an important virus vector, transmitting Rice stripe virus (RSV), and Rice black-streaked dwarf virus (RBSDV). Insect symbionts play an essential role in the insect fitness, however, it is still unclear about their contributions to viral transmission by SBPH. Here, we investigated endosymbiont communities in non-viruliferous, RSV-infected, and RBSDV-infected SBPH populations using Illumina 16S rRNA gene MiSeq sequencing. In total, 281,803 effective sequences of the 16S rRNA gene were generated from different samples...
May 4, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28466971/apoptosis-in-acanthamoeba-castellanii-belonging-to-the-t4-genotype
#12
Abdul M Baig, Salima Lalani, Naveed A Khan
Here we describe features of apoptosis in unicellular Acanthamoeba castellanii belonging to the T4 genotype. When exposed to apoptosis-inducing compounds such as doxorubicin, A. castellanii trophozoites exhibited cell shrinkage and membrane blebbing as observed microscopically, DNA fragmentation using agarose gel electrophoresis, and phosphatidylserine (PS) externalization using annexin V immunostaining. Overall, these findings suggest the existence of apoptosis in A. castellanii possibly mediated by intrinsic apoptotic cascade...
May 3, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28464298/yield-cultivation-of-magnetotactic-bacteria-and-magnetosomes-a-review
#13
REVIEW
Imran Ali, Changsheng Peng, Zahid M Khan, Iffat Naz
Magnetotactic bacteria (MTB) have started to be employed for the biosynthesis of magnetic nanoparticles, due to the rapidly increasing demand for nanoparticles in biomedical, biotechnology and environmental protection. MBT are the group of prokaryotes that have the ability to produce bio-magnetic minerals or bio-magnetic crystals of either magnetite (Fe3 O4 ) or greigite (Fe3 S4 ) in numerous shapes and size ranges, known as magnetosomes (MS). MS compel MTB to respond to the applied external magnetic field...
May 2, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28429489/transcriptional-analysis-of-the-adaptation-of-ustilago-maydis-during-growth-under-nitrogen-fixation-conditions
#14
José Alejandro Sánchez-Arreguin, Miguel Angel Hernandez-Oñate, Claudia Geraldine León-Ramirez, José Ruiz-Herrera
Regulation of genes involved in nitrogen metabolism likely plays a role in the ability of fungi to exploit and survive under different environmental situations. To learn about the mechanism of adaptation of the biotrophic fungus Ustilago maydis from a medium containing a source of fixed nitrogen, to a medium depending on the ability to fix N2 by its bacterial endosymbiont, we explored gene expression profiles using RNA-Seq analyses under these two conditions. The differentially expressed (DE) fungal genes were analyzed, identifying 90 genes that were regulated 24 h after shifting the fungus to media lacking ammonium nitrate as a nitrogen source...
April 21, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28422446/fusion-of-a-family-20-carbohydrate-binding-module-cbm20-with-cyclodextrin-glycosyltransferase-of-geobacillus-sp-chb1-improves-catalytic-efficiency
#15
Xianbo Jia, Yonghua Guo, Xinjian Lin, Minsheng You, Chenqiang Lin, Longjun Chen, Jichen Chen
Cyclodextrin glycosyltransferase (CGTase) is an important industrial enzyme for production of cyclodextrins (CDs) from starch by intramolecular transglycosylation. CGTase consists of five domains labeled A to E. For optimizing catalytic activity of CGTase, CGTase of Geobacillus sp. was fused with the family 20 carbohydrate-binding module (CBM) of the Bacillus circulans strain 251 CGTase. The CBMbc251 that has a low binding free energy with maltohexaose, was selected by in silico design. Then the fusion enzyme, CGTΔE-CBMbc251, was constructed by fusing the CBMbc251 to the C-terminal region of CGTΔE...
April 19, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28418068/recombinant-overexpression-of-dihydroneopterin-aldolase-catalyst-potentially-regulates-folate-biofortification
#16
Dhananjay K Pandey, Atul Kumar, Jitendra S Rathore, Nagendra Singh, Bhupendra Chaudhary
We aim to investigate the prospects of increased production of folate through the overexpression of heterologous dihydroneopterin aldolase catalyst. The gene encoding aldolase catalyst was cloned into an expression vector and the induced recombinant protein was purified through metal-affinity chromatography which appeared at 14 kDa position on polyacrylamide-gel. Remarkably, a periodic increase in the extracellular and intracellular folic acid concentration was observed at 4 h growth of induced recombinant DHNA samples than control in a pH-dependent manner...
April 18, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28407285/spatial-variation-in-bacterial-community-in-natural-wetland-river-sea-ecosystems
#17
Hongxia Zhang, Shiling Zheng, Jiawang Ding, Oumei Wang, Fanghua Liu
Wetland-estuarine-marine environments are typical oxic/anoxic transition zones and have complex water flow-paths within the zone of mixing where freshwater interacts with ocean water. Little is known about the impact of this interaction on bacterial community structures or the relationship between bacterial community and geochemical factors in such transitional mixing environments. Hence, we investigated the distribution patterns and diversity in bacterial communities in the Yellow River estuary-coastal wetland-Bohai Sea transition zone by analyzing 39 samples from 13 ordered sites...
April 13, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28407275/agriculturally-important-microbial-biofilms-present-status-and-future-prospects
#18
REVIEW
Kulandaivelu Velmourougane, Radha Prasanna, Anil Kumar Saxena
Microbial biofilms are a fascinating subject, due to their significant roles in the environment, industry, and health. Advances in biochemical and molecular techniques have helped in enhancing our understanding of biofilm structure and development. In the past, research on biofilms primarily focussed on health and industrial sectors; however, lately, biofilms in agriculture are gaining attention due to their immense potential in crop production, protection, and improvement. Biofilms play an important role in colonization of surfaces - soil, roots, or shoots of plants and enable proliferation in the desired niche, besides enhancing soil fertility...
April 13, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28397264/population-densities-of-indigenous-acidobacteria-change-in-the-presence-of-plant-growth-promoting-rhizobacteria-pgpr-in-rhizosphere
#19
Sadaf Kalam, Subha Narayan Das, Anirban Basu, Appa Rao Podile
Rhizosphere microbial community has diverse metabolic capabilities and plays a crucial role in maintaining plant health. Oligotrophic plant growth promoting rhizobacteria (PGPR), along with difficult-to-culture microbial fractions, might be involved synergistically in microbe-microbe and plant-microbe interactions in the rhizosphere. Among the difficult-to-culture microbial fractions, Acidobacteria constitutes the most dominant phylum thriving in rhizospheric soils. We selected effective PGPR for tomato and black gram and studied their effect on population densities of acidobacterial members...
April 11, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28397262/extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-and-klebsiella-pneumoniae-critical-tools-for-antibiotic-resistance-pattern
#20
REVIEW
Nagarajan Padmini, Antony Alex Kennedy Ajilda, Natesan Sivakumar, Gopal Selvakumar
Drug resistance is a phenomenon where by an organism becomes fully or partially resistant to drugs or antibiotics being used against it. Antibiotic resistance poses an exacting intimidation for people with underlying medical immune conditions or weakened immune systems. Infections caused by the enzyme extended spectrum β-lactamase (ESBL) producing multi drug resistance (MDR) Enterobacteriaceae especially Escherichia coli and Klebsiella pneumoniae are resistant to a broad range of beta lactams, including third generation cephalosporins...
April 11, 2017: Journal of Basic Microbiology
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