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Journal of Basic Microbiology

Baoshen Zhang, Yanhua Wang, Jing Miao, Yang Lu, Ruiyang Lu, Xiaoqiang Sun, Wangtai Luo, Xiaoyan Chi, Zhibin Feng, Yihe Ge
In previous studies with Pseudomonas chlororaphis G05, two operons (phzABCDEFG and prnABCD) were confirmed to respectively encode enzymes for biosynthesis of phenazine-1-carboxylic acid and pyrrolnitrin that mainly contributed to suppression of some fungal phytopathogens. Although some regulators were identified to govern their expression, it is not known how two operons coordinately interact. By constructing the phz- or/and prn- deletion mutants, we found that in comparison with the wild-type strain G05, phenazine-1-carboxylic acid production in the mutant G05Δprn obviously decreased in GA broth in the absence of prn, and pyrrolnitrin production in the mutant G05Δphz remarkably declined in the absence of phz...
July 11, 2018: Journal of Basic Microbiology
Aleksey A Vatlin, Olga B Bekker, Ludmila N Lysenkova, Andrey E Shchekotikhin, Valery N Danilenko
We describe Streptomyces fradiae mechanisms of sensitivity to nitrone-oligomycin A, a derivative of oligomycin A. We obtained S. fradiae-nitR+ bld, a nitrone-oligomycin A resistant mutant with a «bald» phenotype. Comparative genomic analysis of the wild-type S. fradiae ATCC19609 and S. fradiae-nitR+ bld revealed a mutation in padR - a gene encoding a multifunction transcription regulator, which resulted in the amino acid replacement in a highly conserved DNA-binding domain. Bioinformatics genome analysis of S...
July 2, 2018: Journal of Basic Microbiology
Ming Zhao, Xin-Yi Tao, Feng-Qing Wang, Yu-Hong Ren, Dong-Zhi Wei
The lac operon is a delicate inducible gene expression element in bacteria. To efficiently induce gene expression, a sufficient dosage of an inducer, usually that of 500-1000 µM isopropyl β-D-1-thiogalactopyranoside (IPTG), is required to keep repressor LacI from its binding sites, which is a heavy cost burden in low-value-added products. So we propose a strategy to reduce the required dosage of IPTG by restricting LacI expression. To test this strategy, we employed a reconstructed IPTG inducible expression system based on lac operon, Promoter(lacO)-target gene-PtacL-lacI, where a modified promoter, Ptac, with a random synthetic library (PtacL) to instead of PlacI to optimize LacI expression in Escherichia coli...
July 2, 2018: Journal of Basic Microbiology
Khalid Salah El Dein Abdel-Lateif, Samira R Mansour, Mohamed F El-Badawy, Mohamed M Shohayeb
Frankia strains isolated from Saudi Arabia, reported for the first time, were identified based on the morphological and molecular tools compared to those isolated from Egypt. All strains displayed typical morphological characterization of Frankia strains represented by branched hyphae, production of vesicles and sporangia. The phylogenetic analysis and relationships among Frankia strains were investigated by comparing 16S rRNA gene sequences. The analysis revealed three genetic groups which formed two clusters...
July 1, 2018: Journal of Basic Microbiology
Librada A Atencio, Francesco Dal Grande, Giannina Ow Young, Ronnie Gavilán, Héctor M Guzmán, Imke Schmitt, Luis C Mejía, Marcelino Gutiérrez
Pseudoalteromonas is a genus of marine bacteria often found in association with other organisms. Although several studies have examined Pseudoalteromonas diversity and their antimicrobial activity, its diversity in tropical environments is largely unexplored. We investigated the diversity of Pseudoalteromonas in marine environments of Panama using a multilocus phylogenetic approach. Furthermore we tested their antimicrobial capacity and evaluated the effect of recombination and mutation in shaping their phylogenetic relationships...
June 25, 2018: Journal of Basic Microbiology
Hüsniye T Yalçın, Gülşah Ergin-Tepebaşı, Ebru Uyar
In this study, 65 yeast strains were isolated from different environmental samples contaminated with various petroleum hydrocarbons such as activated sludges and soil samples from automobile workshops. The yeast isolates were tested for biosurfactant production using various screening methods such as parafilm M test, oil displacement assay, drop collapse assay, determination of surface tension reduction, and emulsification index. Nineteen of the isolates were found positive for biosurfactant production and their molecular characterizations were carried out by sequencing analysis of the ITS1-5...
June 25, 2018: Journal of Basic Microbiology
Pintubala Kshetri, Subhra S Roy, Susheel K Sharma, Thangjam S Singh, Meraj A Ansari, Blessa Sailo, Surender Singh, Narendra Prakash
Twelve actinobacterial strains were isolated from tomato rhizospheric soil from Manipur, a state in North East Indian Himalayan Region and screened for keratinolytic and plant growth promoting traits. Nine promising isolates were identified as Streptomyces species using partial 16S rRNA gene sequencing. Among the seven isolates showing chicken feather degradation activity, three keratinolytic strains RCM-SSR-2, -6, and -12 were found to be the most efficient feather degrading strains achieving 90% feather weight loss within 48 h of incubation...
June 25, 2018: Journal of Basic Microbiology
Kamel Elhalag, Mohamed Nasr-Eldin, Ahmed Hussien, Abdelmonim Ahmad
A new podovirus RsPod1EGY Ralstonia phage (GenBank accession no MG711516) with a specific action against R. solanacearum phylotype IIa, sequevar I (race 3, biovar 2) was isolated from Egyptian soil. The potential efficacy of the isolated phage to be used as biocontrol agent was evaluated in vitro and under greenhouse conditions. The podovirus phage produced a plaque size of 3.0-4.0 mm in diameter and completed its infection cycle in 180 min after infection with a burst size of ∼27 virions per infected cell...
June 25, 2018: Journal of Basic Microbiology
Biranchi N Mishra, Manish S Kaushik, Gerard Abraham, Pawan K Singh
Spore (akinete) formation in the heterocystous and branched filamentous cyanobacterium Fischerella muscicola involves a significant increase in cell size and formation of several endospores in each of the cells. In present study, the physico-chemical factors (pH, light sources, nutrient deficiency, nitrogen sources, carbon sources, and growth hormones) affecting the germination of spores of F. muscicola were examined. Increase in spore germination frequency was detected above pH 8 with maximum germination (46...
June 19, 2018: Journal of Basic Microbiology
Qi Wang, Qingdong Xie, Linyan He, Xiafang Sheng
In this study, we collected different levels of altered rocks of a rocky mountain and the adjacent soil and characterized the abundance and weathering effectiveness of Bacillus strains. Based on qPCR and culture-dependent approaches, the gene copies or the numbers of Bacillus strains were significantly higher in the soil than in the altered rocks, while the ratio of the gene copies or the numbers of Bacillus strains to those of total bacteria was higher in the less altered rock, followed by the more altered rock and the soil...
June 14, 2018: Journal of Basic Microbiology
Zhengying Shao, Zhang Li, Yanhui Fu, Yangping Wen, Saijin Wei
The induced resistance against plant pathogens via biocontrol agents is considered as an eco-friendly and promising strategy. In this study, the induced resistance against Magnaporthe oryzae (M. oryzae) in rice seedling by a new potential biocontrol agent Streptomyces JD211 (JD211) was evaluated. The effects of JD211 on defense-related enzymes activities and defense genes expression were investigated. The biocontrol efficacy of different JD211 concentrations was different, and the treatment of 10 g kg-1 JD211 achieved the highest biocontrol efficacy...
June 14, 2018: Journal of Basic Microbiology
Xiaoyan Lin, Yingfang Bian, Renxiang Mou, Zhaoyun Cao, Zhenzhen Cao, Zhiwei Zhu, Mingxue Chen
Ustilaginoidea (U.) virens grows on rice grains and leads to significant rice yield losses in most of the major rice producing areas. Meanwhile, ustiloxins produced by U. virens are a serious hazard to human health and ecological safety of farmlands. The other key point is that ustiloxins have been regarded as a novel resource with their potential in the treatment of cancers. There is no better way to extract ustiloxins than from pure culture of the high ustilotoxin-producing strains. U. virens has become a key research organism...
June 13, 2018: Journal of Basic Microbiology
Shangqi Xu, Jianfeng Zhang, Shasha Luo, Xue Zhou, Shaohua Shi, Chunjie Tian
Soil microbes play critical roles in global biogeochemical cycles, but their succession patterns across long temporal scales have rarely been studied. In this study, soil samples were collected from three volcanoes in Wudalianchi, northeastern China: Laoheishan (LH, approximately 240 years old), Dongjiaodebushan (DJ, 0.45-0.6 million years old), and Nangelaqiushan (NG, 0.8-1.3 million years old). For each volcano, both southern (S) and northern (N) slope aspects were sampled. Soil microbial communities were analyzed using phospholipid fatty acid analysis (PLFA)...
June 8, 2018: Journal of Basic Microbiology
Tuheng Wu, Zhiwei Ye, Liqiong Guo, Xueqin Yang, Junfang Lin
To understand molecular mechanism of cold-induced fruiting in Flammulina velutipes, which is one of most popular edible fungi in east Asia, de novo assembly of the F. velutipes transcriptome was carried out. There were 26,888,494 and 26,275,146 clean reads obtained from mycelium and primordia of F. velutipes, respectively. A total of 20,157 unigenes were de novo assembled and 15,058 of them were annotated. Moreover, 7935 unigenes were differentially expressed between mycelium and primordia, 4025 of them were up-regulated and 3910 were down-regulated...
June 6, 2018: Journal of Basic Microbiology
Li Ai, Yaping Huang, Chuan Wang
A halophilic lipase (LipS2) was produced by Chromohalobacter canadensis strain which was isolated from ancient salt well of Zigong, China. LipS2 was purified to homogeneity and showed a single band with molecular mass of 58 kDa by SDS-PAGE. LipS2 preferred middle-to-long acyl chain esters with C14 triglycerides as optimum substrate. It was noteworthy that LipS2 displayed efficient hydrolysis activity to some vegetable oils which were composed of polyunsaturated fatty acid. LipS2 showed high activity in range of 2...
June 4, 2018: Journal of Basic Microbiology
Albin Alfreider, Teresa Bogensperger
The analysis of RubisCO genes is a highly useful instrument to explore the diversity of chemoautotrophic bacteria using the Calvin-Benson-Bassham cycle for CO2 fixation. However, because of the wide taxonomic distribution of phylogenetically related RubisCO forms, environmental studies targeting chemoautotrophs are hampered in habitats dominated by phototrophs. Here, we report the development of a gene marker that specifically detects form IA RubisCO genes in bacteria, excluding photoautotrophic representatives...
May 24, 2018: Journal of Basic Microbiology
Venkataseshan Jagannathan, Pragasam Viswanathan
Struvite or infection stones are one of the major clinical burdens among urinary tract infection, which occur due to the interaction between microbes and urine mineral components. Numerous urinary tract infection (UTI) causing microbes regulate through biofilm formation for survival from host defense, it is often found difficult in its eradication with simple anti-microbial agents and also the chance of recurrence and resistance development is significantly high. Cranberry consumption and maintenance of urinary tract health have been supported by clinical, epidemiological, and mechanistic studies...
May 18, 2018: Journal of Basic Microbiology
Elaheh Pourfakhraei, Jalil Badraghi, Fatemeh Mamashli, Mahboobeh Nazari, Ali Akbar Saboury
Petroleum, as the major energy source, is indispensable from our lives. Presence of compounds resistant to degradation can pose risks for human health and environment. Basidiomycetes have been considered as powerful candidates in biodegradation of petroleum compounds via secreting ligninolytic enzymes. In this study a wood-decaying fungus was isolated by significant degradation ability that was identified as Daedaleopsis sp. by morphological and molecular identification methods. According to GC/MS studies, incubation of heavy crude oil with Daedaleopsis sp...
May 18, 2018: Journal of Basic Microbiology
Dominika Thiem, Michał Złoch, Renata Gadzała-Kopciuch, Sonia Szymańska, Christel Baum, Katarzyna Hrynkiewicz
The impact of increasing Cd2+ exposure on the quality and quantity of siderophores produced by a plant growth promoting Pseudomonas fulva strain was tested to gain insight into the degree of change. P. fulva was cultured in the increasing concentrations of Cd2+ (0, 0.5, 1.0, 2.0 mM). The secreted siderophores were separated by HPLC and characterized by UHPLC-QTOF/MS. In the presence of 2 mM Cd2+ synthesis of siderophores (hydroxamates, catecholates, phenolates) was mitigated compared to the treatments with lower concentrations of Cd2+ (0...
May 18, 2018: Journal of Basic Microbiology
Mariathason Birdilla Selva Donio, Subbiahanadar Chelladurai Karthikeyan, Mariavincent Michaelbabu, Ganapathi Uma, Ramaiyan Raja Jeya Sekar, Thavasimuthu Citarasu
Bioemulsifier (BE)-producing Haererehalobacter sp. JS1 was isolated and identified from the solar salt works in India. The BE was extracted, purified, and characterized by Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Emulsification activity was performed against different oils and dye degradation potential against different dyes. The production of BE was optimized using different carbon sources (C), nitrogen sources (N), pH, and NaCl. BE screening methods revealed that, Haererehalobacter sp. JS1 was highly positive BE production...
May 18, 2018: Journal of Basic Microbiology
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