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Journal of Basic Microbiology

Xiao-Xia Ma, Xin Cao, Peng Ma, Qiu-Yan Chang, Lin-Jie Li, Xiao-Kai Zhou, De-Rong Zhang, Ming-Sheng Li, Zhong-Ren Ma
The evolutionary factors in influencing the genetic characteristics of nucleotide, synonymous codon, and amino acid usage of 18 mycoplasma species were analyzed. The nucleotide usage at the 1st and 2nd codon position which determines amino acid composition of proteins has a significant correlation with the total nucleotide composition of gene population of these mycoplasma species, however, the nucleotide usage at the 3rd codon position which affects synonymous codon usage patterns has a slight correlation with either the total nucleotide composition or the nucleotide usage at the 1st and 2nd codon position...
March 14, 2018: Journal of Basic Microbiology
Xiao-Xia Ma, Peng Ma, Qiu-Yan Chang, Zhen-Bin Liu, Derong Zhang, Xiao-Kai Zhou, Zhong-Ren Ma, Xin Cao
Lyme disease, caused by Borrelia burgdorferi, is a focally endemic tick-transmitted zoonotic infection. In this study, the major factors underlying synonymous codon-related amino acid usage in the B. burgdorferi genome and bias in synonymous codon usage of the translation initiation region of coding sequences were analyzed. Additionally, adaptation of B. burgdorferi to several of its hosts was analyzed in the context of synonymous codon usage. Principal component analysis (PCA) revealed that nucleotide content at the third synonymous position of a codon influenced the synonymous codon usage pattern, but the strand-specific factor did not influence the synonymous codon usage pattern of B...
March 13, 2018: Journal of Basic Microbiology
Sheryl O Fernandes, Lankalapalli Surya Prakash, Mechirackal Balan Binish, Kottekkatu Padinchati Krishnan, Palayil John Kurian
Mn-oxidizing potential of two metal-tolerant bacterial strains - Halomonas meridiana and Marinobacter algicola isolated from the South West Indian Ridge waters were compared at varying concentrations of Mn (II), i.e., 1, 10, and 100 μmol and mmol L-1 . Accompanying changes in their morphology and metabolism were also determined. At concentrations >1 mmol L-1 Mn (II), Mn-oxidizing potential of M. algicola was 2-7 times greater than that of H. meridiana. Scanning electron microscopy revealed that exposure to elevated metal content prompted bacterial cells especially those of M...
March 13, 2018: Journal of Basic Microbiology
Yu-Fan Liu, Chao-Tsai Liao, Ying-Chuan Chiang, Chih-En Li, Yi-Min Hsiao
Xanthomonas campestris pv. campestris (Xcc) is the causative agent of black rot in crucifers. Here, one EZ-Tn5 transposon mutant of Xcc, altered in bacterial attachment, was isolated. Further analysis revealed that the transposon was inserted in the wxcX gene (encodes a hypothetical protein) of the transposon mutant. Sequence analysis revealed that WxcX is highly conserved in Xanthomonas, but none has been characterized. In this study, it was indicated that mutation of wxcX resulted in enhanced bacterial attachment, reduced virulence on the host cabbage, and increased sensitivity to sodium dodecyl sulfate...
March 5, 2018: Journal of Basic Microbiology
Lingui Xue, Hai Shang, Ping Ma, Xia Wang, Xiaoyan He, Junbo Niu, Juanli Wu
The aim was to study the effect of artificially constructed consortia of microalgae-bacterial symbionts on growth and lipid production by Chlorella vulgaris (C. vulgaris), as well as the inter-relationship between microalgae and bacterial in a photoautotrophic system. The results showed that compared to an axenic culture of C. vulgaris, H1 co-culture system (axenic C. vulgaris-Stenotrophomona smaltophilia) had the strongest effect on the C. vulgaris growth. The biomass, specific growth rate and maximum productivity of C...
February 28, 2018: Journal of Basic Microbiology
Jia Xu, Joseph W Kloepper, Ping Huang, John A McInroy, Chia H Hu
The aims of this study were to isolate and characterize N2 -fixing bacteria from giant reed and switchgrass and evaluate their plant growth promotion and nutrient uptake potential for use as biofertilizers. A total of 190 bacteria were obtained from rhizosphere soil and inside stems and roots of giant reed and switchgrass. All the isolates were confirmed to have nitrogenase activity, 96.9% produced auxin, and 85% produced siderophores. Then the top six strains, including Sphingomonas trueperi NNA-14, Sphingomonas trueperi NNA-19, Sphingomonas trueperi NNA-17, Sphingomonas trueperi NNA-20, Psychrobacillus psychrodurans NP-3, and Enterobacter oryzae NXU-38, based on nitrogenase activity, were inoculated on maize and wheat seeds in greenhouse tests to assess their potential benefits to plants...
February 23, 2018: Journal of Basic Microbiology
Abhas K Maharana, Shiv M Singh
Psychrotolerant yeast Rhodotorula sp. Y-23 was isolated from the sediment core sub-samples of Nella Lake, East Antarctica. Isolate was screened for lipase production using plate assay method followed by submerged fermentation. Production optimization revealed the maximum lipase production by using palmolein oil (5% v/v), pH 8.0 and inoculum size of 2.5% v/v at 15 °C. The potential inducers for lipase were 1% w/v of galactose and KNO3, and MnCl2(0.1% w/v). Final productions with optimized conditions gave 5...
February 14, 2018: Journal of Basic Microbiology
María A Correa Deza, Gladys I Martos, Marta Nuñez, Mario Fiori, Carla L Gerez, Graciela Font
In artisanal tanneries, the skins are immersed in cereals fermented by natural microbial flora in order to reduce the pH of the skin, an essential condition for carrying out the final step. The environmental thermal variation alters the fermentation process and affects the quality of the final product. The aim of this work was to isolate lactic acid bacteria from cereals mixture fermented in an artisanal tannery and to evaluate in vitro the acidifying activity of the strains as a first step for the formulation of a starter culture...
February 12, 2018: Journal of Basic Microbiology
Alexander V Lisov, Oksana V Belova, Nataliya G Vinokurova, Tatiana V Semashko, Anatolii G Lobanok, Alexey A Leontievsky
This work investigated the regulatory role of the interaction between cellobiose dehydrogenase (CDH) and β-glucosidase (β-GLU) in the conversion of cellobiose into cellobionolactone or glucose in vitro. To study the regulation, the two enzymes were isolated from the culture medium of the fungus Cerrena unicolor grown on a medium with microcrystalline cellulose. The enzymes were obtained in an electrophoretically homogeneous state. Their properties were studied. Both enzymes had acidic pH optima and were more stable in the acidic pH range...
February 8, 2018: Journal of Basic Microbiology
Wenjie Di, Wu Qu, Runying Zeng
AgaM1, a β-agarase belonging to glycoside hydrolases family 16 (GH16), was cloned from the environmental DNA of mangrove sediments. The gene agaM1 is 2136 bp in length and encodes a protein of 712 amino acids. The properties of recombinant AgaM1 (rAgaM1) were studied using prokaryotic expression. The optimum temperature and pH were 50 °C and 7.0, respectively, and rAgaM1 exhibited a high adaptability to wide ranges of temperature and pH. A relatively high activity was retained at from 30 to 60 °C and from pH 6...
February 7, 2018: Journal of Basic Microbiology
Subramanian Muthamil, Vivekanandham Amsa Devi, Boopathi Balasubramaniam, Krishnaswamy Balamurugan, Shunmugiah Karutha Pandian
Candida species are opportunistic fungal pathogens, which are known for their biofilm associated infections on implanted medical devices in clinical settings. Broad spectrum usage of azole groups and other antifungal agents leads to the occurrence of drug resistance among Candida species. Most of the antifungal agents have failed to treat the biofilm mediated Candida infections. In the present study, silver nanoparticles (AgNPs) were synthesized using Dodonaea viscosa and Hyptis suoveolens methanolic leaf extracts and characterized by ultraviolet-visible absorption spectroscopy, X-ray diffraction analysis, Fourier transform infrared spectroscopy and Scanning electron microscopy, Dynamic light scattering, and Zeta potential analysis...
February 7, 2018: Journal of Basic Microbiology
Chang-Ro Lee, Won-Jae Chi, Ju-Hyeon Lim, Vijayalakshmi Dhakshnamoorthy, Soon-Kwang Hong
The sco6546 gene of Streptomyces coelicolor A3(2) was annotated as a putative glycosyl hydrolase belonging to family 48. It is predicted to encode a 973-amino acid polypeptide (103.4 kDa) with a 39-amino acid secretion signal. Here, the SCO6546 protein was overexpressed in Streptomyces lividans TK24, and the purified protein showed the expected molecular weight of the mature secreted form (934 aa, 99.4 kDa) on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. SCO6546 showed high activity toward Avicel and carboxymethyl cellulose, but low activity toward filter paper and β-glucan...
February 5, 2018: Journal of Basic Microbiology
Zhenyuan Zhu, Qiaoying Song, Fengying Dong
The objective of this study was to investigate the screening, taxonomy characterization, Pb biosorption, and application of the high Pb-resistant fungus F1 separated from the heavy metal contaminated soil. Fungus F1 was screened through metal concentration gradient ranging from 25 to 4000 mg L-1 . The internal transcribed spacers (ITS) of the strain was analyzed by molecular biotechnology. The adsorption conditions were also evaluated. The precipitation of fungus F1 was analyzed by Scanning Electron Microscopy (SEM), Fourier transformer infrared spectroscopy (FTIR) and energy dispersive X-ray (EDX) techniques...
February 2, 2018: Journal of Basic Microbiology
Yayun Wang, Kei Okugawa, Emi Kunitake, Makiko Sakka, Tetsuya Kimura, Kazuo Sakka
Although Ruminiclostridium josui (formerly Clostridium josui), a strictly anaerobic mesophilic cellulolytic bacterium, is a promising candidate for biomass utilization via consolidated bioprocessing, its host-vector system has not yet been established. The existence of a restriction and modification system is a significant barrier to the transformation of R. josui. Here, we partially purified restriction endonuclease RjoI from R. josui cell extract using column chromatography. Further characterization showed that RjoI is an isoschizomer of DpnI, recognizing the sequence 5'-Gmet ATC-3', where the A nucleotide is Dam-methylated...
February 1, 2018: Journal of Basic Microbiology
Binish Mechirackal Balan, Sruthy Shini, Kottekkattu P Krishnan, Mahesh Mohan
Mercury tolerant bacteria Pseudarthrobacter oxydans strain MM20 and Pseudomonas frederiksbergensis strain SS18 were isolated from the tundra ecosystem of Ny-Ålesund, Svalbard, where commercial exploitation of the coal existed till 1960s. Minimum inhibitory concentration (MIC), mercury removal, mercury biosorption, and antibiotic resistance of these strains were analyzed. P. frederiksbergensis strain SS18 showed high tolerance (2.0 ppm) to mercury than P. oxydans strain MM20 (1.5 ppm). Mercury removal and biosorption studies were carried out in liquid media containing 1...
January 31, 2018: Journal of Basic Microbiology
Kiran G Bapatla, Arjun Singh, Srujana Yeddula, Ramanagouda H Patil
The insect gut has been the house of many taxonomically and physiologically diverse groups of microbial colonizers as symbionts and commensals, which are evolving to support the physiological requirement of insects. Lepidoptera is one of the important family of class hexapoda, comprising agriculture insect pest Spodoptera litura and Spilosoma obliqua. Information on gut microbiota and their functional role in these insects was meager to elucidate the wide-ranging survivalist mechanisms. In this context, we analyzed the composition, diversity and functional role of gut bacteria in S...
January 30, 2018: Journal of Basic Microbiology
Lijun Xi, Dejian Liu, Lingling Wang, Nenghu Qiao, Jianguo Liu
Catechol 2,3-dioxygenase (C23O) from a new phenolic compound degrader Thauera sp. K11 was purified and characterized. The native form of the enzyme was determined as a homotetramer with a molecular weight of 140 kDa, and its isoelectric point was close to 6.4. One iron per enzyme subunit was detected using atom absorption spectroscopy, and the effective size of C23O in its dilute solution (0.2 g L-1 , pH 8.0) was 14.5 nm. The optimal pH and temperature were 8.4 and 45 °C, respectively. The addition of Mg2+ , Cu2+ , Fe2+ , and Mn2+ could improve the enzyme activity, while Ag+ was found to be a strong inhibitor...
January 30, 2018: Journal of Basic Microbiology
Qiang Li, Chuan Xiong, Xiaolin Li, Xin Jin, Wenli Huang
Tricholoma matsutake (S. Ito et Imai) is an ectomycorrhizal basidiomycete associated with Pinaceae and Fagaceae trees in the Northern Hemisphere. It is still unknown whether the symbiotic relationship with this ectomycorrhiza could affect the host plant's endophytic microbial community. In this study, we used high throughput sequencing to analyze the endophytic microbial communities of different Quercus aquifolioides tissues with or without T. matsutake partner. About 35,000 clean reads were obtained per sample, representing 34 bacterial phyla and 7 fungal phyla...
January 23, 2018: Journal of Basic Microbiology
Sajida Munir, Nazia Jamil
Mixed bacterial cultures are increasingly being used in the production of polyhydroxyalkanoates (PHAs), as they have the potential to be more cost effective than axenic pure cultures. The purpose of this study was to use pure cultures in combination to identify their potential of PHA production. In this work we used volatile fatty acids (VFAs) and glucose as carbon source to check the ability of selected strains ST2 (Pseudomonas sp.) and CS8 (Bacillus sp.) as co-culture. The production of PHA in pure co-cultures of bacteria was therefore investigated in order to understand the effect of combining cultures on PHA production parameters and material properties...
January 4, 2018: Journal of Basic Microbiology
Yuanyuan Lu, Shuqin Xiao, Mingyue Yuan, Yibo Gao, Jiaying Sun, Chunsheng Xue
Overlap-extension PCR is a method for splice of gene segments to produce focused fragments for constructing recombinant plasmid, but its complexity limits its application. To simplify the protocol and to improve the effectiveness, we employed gradient temperatures to replace the single annealing temperature in the thermo-cycling program, and optimize the templates ratio. The concentration of each fragment was adjusted to 10 ng µl-1 . Fragment concentration ratio was the inverse of the fragment size ratio...
January 2, 2018: Journal of Basic Microbiology
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