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Journal of Basic Microbiology

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https://www.readbyqxmd.com/read/28295433/structural-analysis-and-enhanced-production-of-fusaricidin-from-paenibacillus-kribbensis-cu01-isolated-from-yellow-loess
#1
Jaewon Ryu, Jong M Kim, Chul W Lee, Si W Kim
A bacterial strain showing strong antifungal activity was isolated from yellow loess and was identified as Paenibacillus kribbensis CU01. Insoluble mucoidal polymers were separated from M9 culture medium via low-speed centrifugation. Most antifungal activity was associated with substances in the insoluble precipitate, which was purified by reverse phase high performance liquid chromatography. Purified fractions were analyzed using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry...
March 10, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28272809/bioprospection-of-cold-adapted-yeasts-with-biotechnological-potential-from-antarctica
#2
María Martha Martorell, Lucas Adolfo Mauro Ruberto, Pablo Marcelo Fernández, Lucía Inés Castellanos de Figueroa, Walter Patricio Mac Cormack
The aim of this study was to investigate the ability to produce extracellular hydrolytic enzymes at low temperature of yeasts isolated from 25 de Mayo island, Antarctica, and to identify those exhibiting one or more of the evaluated enzymatic activities. A total of 105 yeast isolates were obtained from different samples and 66 were identified. They belonged to 12 basidiomycetous and four ascomycetous genera. Most of the isolates were ascribed to the genera Cryptococcus, Mrakia, Cystobasidium, Rhodotorula, Gueomyces, Phenoliferia, Leucosporidium, and Pichia...
March 8, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28272747/a-simple-rapid-and-sensitive-plate-assay-for-detection-of-microbial-hyaluronidase-activity
#3
Sandip Patil, Bhushan Chaudhari
Hyaluronidase (hyase) is a glycosidase enzyme that predominantly degrades hyaluronic acid (HA) having important applications in many biotechnological processes and therapeutics. Several assay methods have been proposed to screen hyase producing microorganisms; however, they rely on unique reagents and sophisticated instruments, which are expensive and could be unavailable in general laboratories. In the present studies, a rapid, simple, sensitive, highly reproducible, and cost-effective qualitative plate assay has been developed for the screening of hyase producing microorganisms...
March 8, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28225559/effects-of-an-alternative-oxidase-gene-on-conidia-viability-under-external-stresses-in-monascus-ruber-m7
#4
Yanchun Shao, Qi Li, Youxiang Zhou, Fusheng Chen
Monascus species can produce natural edible pigments and many other bioactive metabolites. In this study, mraox gene (Monascus ruber alternative oxidase) was isolated, sequenced, and replaced in order to investigate the function in resistance of conidia to stressful conditions. The derived protein of the mraox gene consisted of 350 amino acids with a conserved ferritin-like diiron-binding domain at the C-terminus, sharing a high homolog with alternative oxidase proteins in other filamentous fungi. Deletion of mraox gene repressed the conidia germination rate (CGR) when conidia were exposed to H2 O2 , high temperature (40 and 50 °C) and alkerline buffer (pH8...
February 22, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28217898/metal-induced-changes-in-trivalent-chromium-resistant-alcaligenes-faecalis-vitsim2
#5
Shiny C Matilda, Chittibabu Shanthi
The changes induced in bacterial strains under stress conditions provide an insight into metal resistance strategies. Trivalent chromium resistant bacterium were isolated and identified by 16S rRNA gene sequencing and designated as Alcaligenes faecalis VITSIM2. The growth pattern was monitored. The organism also showed resistance to copper, cadmium, and certain antibiotics. The differentially expressed proteins in SDS PAGE were identified by mass spectrometry as flagellin and 50S ribosomal L36 protein. The morphological changes were identified by scanning electron microscopy...
February 20, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28217886/the-use-of-newly-isolated-streptomyces-strain-tn258-as-potential-biocontrol-agent-of-potato-tubers-leak-caused-by-pythium-ultimum
#6
Imen Sellem, Mohamed Ali Triki, Lobna Elleuch, Manel Cheffi, Ahlem Chakchouk, Slim Smaoui, Lotfi Mellouli
An actinomycete strain designated TN258, was isolated from Tunisian Sahara soil and selected for its antagonistic activity, especially against Pythium ultimum (P. ultimum) causing potato tubers leak. Based on the results of cultural characteristic of TN258 strain, the 16S rRNA gene nucleotide sequence (1433 bp, accession n° HE600071) and the phylogenetic analysis, we propose the assignment of our new isolate bacterium as Streptomyces TN258 strain. After culture optimization, the inhibitory effect of TN258 free cell supernatant against P...
February 20, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28211948/yield-and-cold-storage-of-trichoderma-conidia-is-influenced-by-substrate-ph-and-storage-temperature
#7
Johanna M Steyaert, Anastasia Chomic, Maria Nieto-Jacobo, Artemio Mendoza-Mendoza, Amanda J Hay, Mark Braithwaite, Alison Stewart
In this study we examined the influence of the ambient pH during morphogenesis on conidial yield of Trichoderma sp. "atroviride B" LU132 and T. hamatum LU593 and storage at low temperatures. The ambient pH of the growth media had a dramatic influence on the level of Trichoderma conidiation and this was dependent on the strain and growth media. On malt-extract agar, LU593 yield decreased with increasing pH (3-6), whereas yield increased with increasing pH for LU132. During solid substrate production the reverse was true for LU132 whereby yield decreased with increasing pH...
February 17, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28211942/antifungal-activity-change-of-streptomyces-rimosus-my02-mediated-by-confront-culture-with-other-microorganism
#8
Jicheng Yu, Qiu Liu, Chao Chen, Xiaohui Qi
Streptomyces rimosus can produce antibacterial and antifungal antibiotics, which have important applications in medicine and agriculture. Seventy-nine microbial strains were employed to assay interaction between S. rimosus MY02 and different fungi, actinomyces, and bacteria when confront cultured on solid media. The results showed that the presence of a microorganism might affect the activity of another one. When S. rimosus MY02 confront cultured with other microorganisms, the inductive effect might be positive or negative...
February 17, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28186324/isolation-and-identification-of-n-butyl-tetrahydro-5-oxofuran-2-carboxamide-produced-by-bacillus-sp-l60-and-its-antifungal-activity
#9
Yong-Seong Lee, Jeong-Yong Cho, Jae-Hak Moon, Kil-Yong Kim
Rhizoctonia solani is the cause of substantial economic loss in many crops. The aim of this study is to investigate biocontrol potential of Bacillus sp. L60 against R. solani and to purify an antifungal compound. In this study, Bacillus sp. L60 demonstrated significant antagonism toward R. solani with the dual culture assay. The antifungal compound was extracted from Bacillus sp. L60 culture supernatant with n-butanol, and identified as N-butyl-tetrahydro-5-oxofuran-2-carboxamide (BT-5O-2C) having molecular weights of 185...
February 10, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28156004/comparative-analysis-of-crispr-cas-systems-in-klebsiella-genomes
#10
Juntao Shen, Li Lv, Xudong Wang, Zhilong Xiu, Guoqiang Chen
Prokaryotic CRISPR-Cas system provides adaptive immunity against invasive genetic elements. Bacteria of the genus Klebsiella are important nosocomial opportunistic pathogens. However, information of CRISPR-Cas system in Klebsiella remains largely unknown. Here, we analyzed the CRISPR-Cas systems of 68 complete genomes of Klebsiella representing four species. All the elements for CRISPR-Cas system (cas genes, repeats, leader sequences, and PAMs) were characterized. Besides the typical Type I-E and I-F CRISPR-Cas systems, a new Subtype I system located in the ABC transport system-glyoxalase region was found...
February 3, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28156000/rhamnolipids-from-pseudomonas-aeruginosa-strain-w10-as-antibiofilm-antibiofouling-products-for-metal-protection
#11
Alif Chebbi, Mohamed Elshikh, Farazul Haque, Syed Ahmed, Sara Dobbin, Roger Marchant, Sami Sayadi, Mohamed Chamkha, Ibrahim M Banat
Industrial biofouling-problems associated with the accumulation of microorganisms from flowing water and fluids on processing surfaces can cause severe problems. A Pseudomonas aeruginosa strain W10 was isolated from industrial setting and found to produce predominantly di-rhamnolipids (Rha-Rha-C10-C10) with a yield of around 10 g L(-1) and a critical micelle concentration (CMC) of 80 mg L(-1) . P. aeruginosa W10 rhamnolipids were able to disrupt up to 99% of 48 h pre-formed biofilms of the Gram-positive organisms Bacillus licheniformis CAN55, Staphylococcus capitis SH6, and a mixed culture (strains CAN55, SH6, and W10), under static conditions, at concentrations of 0...
February 3, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28155998/lipids-of-rhodotorula-mucilaginosa-iipl32-with-biodiesel-potential-oil-yield-fatty-acid-profile-fuel-properties
#12
Mahesh Khot, Debashish Ghosh
This study analyzes the single cell oil (SCO), fatty acid profile, and biodiesel fuel properties of the yeast Rhodotorula mucilaginosa IIPL32 grown on the pentose fraction of acid pre-treated sugarcane bagasse as a carbon source. The yeast biomass from nitrogen limiting culture conditions (15.3 g L(-1) ) was able to give the SCO yield of 0.17 g g(-1) of xylose consumed. Acid digestion, cryo-pulverization, direct in situ transesterification, and microwave assisted techniques were evaluated in comparison to the Soxhlet extraction for the total intracellular yeast lipid recovery...
February 3, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28139058/change-in-mineral-weathering-behaviors-of-a-bacterium-chitinophaga-jiangningensis-jn53-under-different-nutrition-conditions
#13
Cheng Cheng, Qi Wang, Linyan He, Xiafang Sheng
Bacteria play important roles in mineral weathering and soil formation. However, little is known about the nutrition-related changes in mineral weathering potential and pattern of bacteria. In this study, mineral weathering behaviors of a novel mineral-weathering bacterium Chitinophaga jiangningensis JN53 were characterized in the presence of three contrasting biotite or potassium feldspar-added media. C. jiangningensis JN53 increased more Fe release from the minerals in the M-BHm (nutrition-poor medium) than in the SSKM (nutrition-rich medium) and BHm (nutrition-moderate medium), while C...
January 31, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28083961/disruption-of-coma-homolog-in-ralstonia-solanacearum-does-not-impair-its-twitching-motility
#14
Anjan Barman, Chandrika Buragohain, Suvendra Kumar Ray
Ralstonia solanacearum is an important phyto-pathogenic bacterium. The bacterium exhibits type IV pili meditated twitching motility that has been implicated in the process of natural transformation in it. A comA gene homolog, alike in several other naturally competent bacteria, has been already reported in this bacterium. However, there are no report of direct link between comA and twitching motility during the natural transformation process in this pathogen. In order to figure out any connection between comA and twitching motility, we created an insertion mutation in comA gene homolog of R...
January 13, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28060404/diversity-and-composition-of-rhizospheric-soil-and-root-endogenous-bacteria-in-panax-notoginseng-during-continuous-cropping-practices
#15
Yong Tan, Yinshan Cui, Haoyu Li, Anxiu Kuang, Xiaoran Li, Yunlin Wei, Xiuling Ji
Rhizobacteria and endophytic bacteria play important roles in protecting host plants from infection by phytopathogens, which cause soil-borne diseases and severely impair plant health. Panax notoginseng is negatively affected by continuous cropping and becomes vulnerable to attack by microbial pathogens. In the present study, culture-independent Illumina MiSeq was used to investigate root-endophytic and rhizospheric bacteria in response to continuous cropping of P. notoginseng. Numbers of rhizospheric bacteria decreased with continuous P...
January 6, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28054716/arsenic-resistance-genes-of-as-resistant-purple-nonsulfur-bacteria-isolated-from-as-contaminated-sites-for-bioremediation-application
#16
Phitthaya Nookongbut, Duangporn Kantachote, Kannan Krishnan, Mallavarapu Megharaj
This study aimed to identify arsenic resistant mechanisms in As-resistant purple nonsulfur bacteria (PNSB) by screening them for presence of As-resistance genes and related enzymes. Resistance to As(III) and As(V) of four As-resistant PNSB determined in terms of median inhibition concentration (IC50 values) were in the order of strains Rhodopseudomonas palustris C1 > R. palustris AB3 > Rubrivivax benzoatilyticus C31 > R. palustris L28 which corresponded to the presence of As-resistance genes in these bacteria...
January 5, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28252211/news-on-journal-of-basic-microbiology
#17
EDITORIAL
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No abstract text is available yet for this article.
March 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28165619/modulation-of-fatty-acids-and-hydrocarbons-in-anabaena-7120-and-its-ntca-mutant-under-calcium
#18
Savita Singh, Ekta Verma, Balkrishna Tiwari, Niveshika, Arun Kumar Mishra
Calcium being a signaling molecule and mediator of cell response, we examined the modulation in fatty acid and hydrocarbon profiles of wild type cyanobacterium Anabaena sp. PCC 7120 and its ntcA mutant under the influence of different calcium chloride concentrations (0-10 mM). Dynamic modifications in fatty acid and hydrocarbon profile were evident through GC-FID analysis of extracted lipids. In the wild type, increase in CaCl2 (10 mM) resulted in unsaturation of fatty acids (observed in terms of high MUFA/PUFA ratio) while hydrocarbon production was distinctly high in the mutant strain compared to wild type at all tested concentrations...
February 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/27874973/degradation-of-benzo-a-pyrene-by-pleurotus-ostreatus-po-3-in-the-presence-of-defined-fungal-and-bacterial-co-cultures
#19
Sourav Bhattacharya, Arijit Das, Muthusamy Palaniswamy, Jayaraman Angayarkanni
Benzo[a]pyrene, a high molecular weight polycyclic aromatic hydrocarbon possesses carcinogenic, teratogenic, and mutagenic properties. The present study focuses on benzo[a]pyrene degradation by Pleurotus ostreatus PO-3, characterization and identification of metabolites produced and the extent of degradation in the presence of axenic culture of P. ostreatus PO-3 and defined co-cultures of the basidiomycete with bacteria and non-basidiomycete fungi. Thin-layer chromatography revealed that P. ostreatus PO-3 transformed benzo[a]pyrene to polar metabolites...
February 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/27862101/characterization-of-thermo-solvent-stable-protease-from-halobacillus-sp-cj4-isolated-from-chott-eldjerid-hypersaline-lake-in-tunisia
#20
Lobna Daoud, Mouna Jlidi, Houda Hmani, Adel Hadj Brahim, Mahdi El Arbi, Mamdouh Ben Ali
About 110 newly isolated halophilic and halotolerant bacteria were screened for protease production. A moderately halophilic strain (CJ4), isolated from Chott Eldjerid Hypersaline lake in Tunisia, showed the highest activity on agar plate and was then selected. The biochemical and physiological characterization of the isolate along with the 16S rRNA sequence analysis placed it in the genus Halobacillus. Protease production was maximal at 120 g/L NaCl (2 M) and it started from the post-exponential phase reaching a maximum level at the early decline phase of bacterial growth...
February 2017: Journal of Basic Microbiology
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