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Journal of Basic Microbiology

Noël Durand, Angélique Fontana, Jean-Christophe Meile, Mirna-Leonor Suàrez-Quiroz, Sabine Schorr-Galindo, Didier Montet
Ochratoxin A (OTA) is a nephrotoxic, teratogenic, immunotoxic, and carcinogenic mycotoxin which is produced in tropical zones mainly by Aspergillus carbonarius, A. niger, A. ochraceus, and A. westerdijkiae. A. ochraceus and A. westerdijkiae species are phenotypically and genomically very close but A. westerdijkiae produce OTA at a very higher level than A. ochraceus. These species have been differentiated recently. The DNA primer pairs which were drawn so far are not specific and a genomic region of the same size is amplified for both species or they are too specific, and in this case, the DNA of a single species is amplified...
September 21, 2018: Journal of Basic Microbiology
Yuridia Ortiz-Rivera, Rogelio Sánchez-Vega, Carlos Horacio Acosta-Muñiz, Néstor Gutiérrez-Méndez, Josefina León-Félix, David R Sepulveda
The influence of environmental factors such as glycerol concentration, time of production, presence of Escherichia coli, and two different strains of Lactobacillus reuteri (ATCC 55730 and ATCC 53608) on 3-hydroxypropionaldehyde (3-HPA) production was analyzed. Additionally, the influence of those factors on gene expression in the 3-HPA production pathway was evaluated. The genes studied were GldC, cbiP, and Lreu_1734. The results of this study showed that the principal environmental factors that influence 3-HPA production are glycerol concentration and Lactobacillus reuteri strain...
September 21, 2018: Journal of Basic Microbiology
Peipei Wang, Zhongqi Liu, Yongchun Huang
A new actinobacteria Streptomyces cavourensis TJ430 was isolated from the mountain soil collected from the southwest of China. In previous study, TJ430 showed striking bactericidal activities and strong ability of antibiotic production. Here, we report complete genome of this bacterium, consisting of 7.6 Mb linear chromosome and 0.2 Mb plasmids. It was predicted 6450 genes in chromosome and 225 genes in plasmids, as well as 12 gene islands in chromosome. Abundant genes have predicted functions in antibiotic metabolism and stress resistance...
September 21, 2018: Journal of Basic Microbiology
Ivana Crnovčić, Manuel Lang, Ingo Ortel, Roderich D Süssmuth, Ullrich Keller
Actinomycin peptide synthetase genes constitute two oppositely oriented transcriptional units, acmADR, and acmBC, separated by a non-coding intergenic region. Gene constructs of the intergenic region together with its adjoining gene acmA or acmB from the actinomycin biosynthetic gene cluster of Streptomyces chrysomallus were transferred into Streptomyces lividans TK64. Each construct expressed the respective synthetase indicating divergent promoters. Primer extension revealed for both directions -10 and -35 boxes similar to σ70 -dependent promoters from Streptomyces and E...
September 20, 2018: Journal of Basic Microbiology
Darina Blagovest Dimitrova-Stefanova, Blagovesta Todorova Gocheva
Three out of 17 Streptomycetes strains - Streptomyces sp. 35 LBG09, Streptomyces sp. 36 LBG09, and Streptomyces sp. 39 LBG09, were selected based on the high production of proteinase inhibitors with trypsin serine proteinase activity. The strains were isolated from soil samples taken from the area around the Bulgarian station on Livingston Island, Antarctica. Biosynthesis of proteinase inhibitors by the promising strains started at different stages of their development but was generally not associated with the growth of the producers...
September 20, 2018: Journal of Basic Microbiology
Yuancheng Qi, Xiankai Sun, Mengke Zhang, Qing Wen, Liyou Qiu, Jinwen Shen
In order to isolate the differentially expressed genes in the primordium stage of Pleurotus ostreatus, the SSH cDNA library was constructed using the cDNA from dikaryotic mycelium stage as a driver and the cDNA from primordium stage as a tester. There were 423 significantly differently expressed clones among 2055 positive clones after three times of reverse Northern blot differential screening. After the repeated sequences being removed, 46 genes were identified which were putatively involved in cell rescue and defense, energy metabolism, transcription and protein regulation, membrane proteins, and signal transduction; 18 genes encoding hypothetical proteins with unknown function; 5 genes without any homology...
September 16, 2018: Journal of Basic Microbiology
Shujun Zhang, Wunier Wunier, Yuan Yao, Morigen Morigen
The Sra protein is a component of the 30S ribosomal subunit while RimJ is a ribosome-associated protein that plays a role in the maturation of the 30S ribosomal subunit. Here we found that Δsra and ΔrimJ cells showed a delayed initiation of DNA replication, prolonged doubling time, decreased cell size, and decreased amounts of total protein and DnaA per cell compared with these observed for wild-type cells. A temperature sensitivity test demonstrated that absence of the Sra or RimJ protein did not change the temperature sensitivity of the dnaA46, dnaB252, or dnaC2 mutants...
September 13, 2018: Journal of Basic Microbiology
Rajesh Prajapati, Shivam Yadav, Neelam Atri
In recent years, release of chemical pollutants has increased due to anthropogenic activities. Heterocystous filamentous cyanobacteria constitute dominant paddy microflora and are excellent biofertilizers augmenting rice productivity. Cyanobacteria are frequently exposed to toxic metals, nickel and arsenic are one of the major toxicants present. We exposed two species of diazotrophic cyanobacteria Anabaena sp. PCC 7120 and Anabaena doliolum, to sub-lethal concentrations (15.0 and 9.0 μM) of Ni2+ and (17...
September 12, 2018: Journal of Basic Microbiology
Zhen-Hai Li, Min Liu, Xiao-Mei Lyu, Feng-Qing Wang, Dong-Zhi Wei
In this study, we focused on the applicability of CRISPR/Cpf1 in genome simplification of Saccharomyces cerevisiae and established a CRISPR/Cpf1 assisted method for rapid markerless large fragment deletion to facilitate laboratory evolution of geome of S. cerevisiae by rational genetic engineering. This method uses a Cpf1 expression plasmid and a crRNA array expression plasmid. The DNA fragment between two DSBs generated by CRISPR/Cpf1 can be cut off from the chromosome, along with re-ligation of the genomic endpoints of the DSBs...
September 9, 2018: Journal of Basic Microbiology
Hafsa Naseem, Muhammad Ahsan, Muhammad A Shahid, Naeem Khan
Drought stress not only effect the population and activities of microorganisms inhabiting the rhizosphere but also various physiological and biochemical process in plants that is, photosynthesis, respiration, translocation, uptake of ions, carbohydrates, and nutrient metabolism. Plant growth promoting rhizobacteria (PGPR) and their exopolysaccharides (EPS) showed profound effects on plant growth and drought tolerance. Reactions of bacteria to drought stress at various organizational levels are different which depends on intensity of stress, duration, species, and growth stage...
September 5, 2018: Journal of Basic Microbiology
Xueran Geng, Jing Fan, Lijing Xu, Hexiang Wang, Tzi Bun Ng
A novel acidic α-galactosidase (EC designated as Leucopaxillus tricolor α-galactosidase (LTG) has been purified to homogeneity from the fruiting bodies of L. tricolor to 855-fold with a specific activity of 956 U mg-1 by the application of chromatography and gel filtration. The molecular mass of LTG was estimated to be 60 kDa as determined by both SDS-PAGE and by gel filtration. The purified enzyme was identified by LC-MS/MS and four inner amino acid sequences were obtained. When 4-nitrophenyl α-D-glucopyranoside (pNPGal) was used as substrate, the optimal pH and optimal temperature of LTG were pH 5...
September 5, 2018: Journal of Basic Microbiology
Vivien Kurucz, Beáta Kiss, Zsuzsa M Szigeti, Gábor Nagy, Erzsébet Orosz, Zoltán Hargitai, Sándor Harangi, Ad Wiebenga, Ronald P de Vries, István Pócsi, Tamás Emri
The physiological background of the unusually high cadmium tolerance (MIC50  > 2 mM) of Aspergillus fumigatus Af293 was investigated. The cadmium tolerance of the tested environmental and clinical A. fumigatus strains varied over a wide range (0.25 mM < MIC50  < 1 mM). Only the Af293 strain showed a MIC50 value of >2 mM, and this phenotype was accompanied by increased in vivo virulence in mice. A strong correlation was found between the cadmium tolerance and the transcription of the pcaA gene, which encodes a putative cadmium efflux pump...
August 31, 2018: Journal of Basic Microbiology
Andrea K Borsodi, Dóra Anda, Judit Makk, Gergely Krett, Péter Dobosy, Gabriella Büki, Anita Erőss, Judit Mádl-Szőnyi
The Buda Thermal Karst System (BTKS) is an extensive active hypogenic cave system located beneath the residential area of the Hungarian capital. At the river Danube, several thermal springs discharge forming spring caves. To reveal and compare the morphological structure and prokaryotic diversity of reddish-brown biofilms developed on the carbonate rock surfaces of the springs, scanning electron microscopy (SEM), and molecular cloning were applied. Microbial networks formed by filamentous bacteria and other cells with mineral crystals embedded in extracellular polymeric substances were observed in the SEM images...
August 30, 2018: Journal of Basic Microbiology
Ping Li, Hong Lin, Zhiqiang Mi, Yigang Tong, Jingxue Wang
Advances in phage therapy and its application require more information on phage genome characteristics and host-phage interaction mechanisms. In this study, a so far unknown T1-like Escherichia coli phage was identified and named vB_EcoS_IME347 (IME347). The genome length of phage IME347 is 50,048 bp with a G + C content of 49.7%. BLASTn alignment showed that the phage has its highest homology (identity 78%, query cover 72%) with phage SRT8 (GenBank: MF996376). Electron microscopy showed that phage IME347 has an icosahedral head and a long non-contractiled tail, features of the family Siphoviridae...
August 26, 2018: Journal of Basic Microbiology
Fouzia Tabssum, Qurat-Ul-Ain Ahmad, Javed Iqbal Qazi
Phenotypic characteristics while complimenting 16S rRNA gene sequencing in identifying bacteria become decisive in solving conflicts of equal % similarity of a given DNA sequence to more than one classified microorganisms. "Phenotypic light" may also indicate right direction when a new species' 16S rDNA sequence is under consideration. In fact 16S rRNA gene sequences give indication that either a novel species has been isolated or the test organism is identified. In each case additional tests are required for resolving different issues...
August 24, 2018: Journal of Basic Microbiology
Xingjia Yin, Guanglong Liu, Lei Peng, Yumei Hua, Xiaoqiong Wan, Wenbing Zhou, Jianwei Zhao, Duanwei Zhu
This study investigated the community structure of ammonia-oxidizing bacteria /archaea (AOB and AOA), as well as the effects of four aquatic plants (namely Ceratophyllum demersum, Hydrilla verticillata, Potamogeton crispus, and Nymphaea tetragona) rhizospheres on the abundance of AOB amoA, AOA amoA, anammox 16S rRNA, nirK, and nirS in Lake Liangzi, China. Phylogenetic analysis revealed that most AOB groups were Nitrosospira and Nitrosomonas, in which Nitrosospira was dominant. The AOA amoA were affiliated with two branches of classical sequences which belonging to Thaumarchaeota: water/sediments branch and soil/sediments branch...
August 17, 2018: Journal of Basic Microbiology
Sajad Babakhani, Mana Oloomi
Transposons are a group of mobile genetic elements that are defined as a DNA sequence. Transposons can jump into different places of the genome; for this reason, they are called jumping genes. However, some transposons are always kept at the insertion site in the genome. Most transposons are inactivated and as a result, cannot move. Transposons are divided into two main groups: retrotransposons (class І) and DNA transposons (class ІІ). Retrotransposons are often found in eukaryotes. DNA transposons can be found in both eukaryotes and prokaryotes...
August 16, 2018: Journal of Basic Microbiology
Emanuel Grassi, Gerardo Robledo, Laura Levin
Six strains belonging to five species of Polyporus (P. arcularius, P. arcularioides, P. tricholoma, P. cfr. tricholoma, and P. varius), collected from an Atlantic Forest area in Misiones (Argentina), where species usually grow exposed to high temperatures and humidity, were identified by morphological and molecular analyses. P. tricholoma (BAFC 4536) and P. arcularioides (BAFC 4534) were selected by their lignin-degrading enzyme production, their ability to produce primordial of basidiomes under submerged fermentation, and the decrease in lignin content caused in Poplar wood (up to 29% after 45 days)...
August 16, 2018: Journal of Basic Microbiology
Lijing Zhou, Yajun Wang, Zhongkui Xie, Yubao Zhang, Sukhdev S Malhi, Zhihong Guo, Yang Qiu, Le Wang
Continuous cropping of lily (Lilium davidii var. unicolor) or any other crop seriously affects yield and quality. In this study, we compared continuous cropping with lily/maize intercropping. We also examined the lily rhizosphere microbes community in both sole lily cropping and lily/maize intercropping systems, by the llumina Miseq platform. Here we refer to data of recent years field experimentation of a lily/maize intercrop system in different planting configurations in the Gaolan Ecological and Agricultural Research Station...
August 12, 2018: Journal of Basic Microbiology
Mananya Martla, Kamontam Umsakul, Kumar Sudesh
Polyhydroxyalkanoates (PHAs) has been paid great attention because of its useful thermoplastic properties and complete degradation in various natural environments. But, at industrial level, the successful commercialization of PHAs is limited by the high production cost due to the expensive carbon source and recovery processes. Pseudomonas mendocina PSU cultured for 72 h in mineral salts medium (MSM) containing 2% (v/v) biodiesel liquid waste (BLW) produced 79.7 wt% poly(3-hydroxybutyrate) (PHB) at 72 h...
August 10, 2018: Journal of Basic Microbiology
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