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Folia Histochemica et Cytobiologica

Sarah Madani, Elara Moudilou, Jean Marie Exbrayat, Sadjia Hammouche
INTRODUCTION: Testicular function in the sand rodent Psammomys obesus is subjected to seasonal alternations with a trigger of spermatogenesis in winter and a total quiescence which extends from late spring to summer. The aim of this study was to investigate the distribution of β-endorphin in the testis at the period of winter sexual activity and at its summer regression, and assess the effect of 17β-estradiol treatment on testicular morphology and β-endorphin expression. MATERIAL AND METHODS: The adult males were grouped into 4 groups (rest group, sexually active group, rest treated with 17β-estradiol group and controls at sexual rest injected with olive oil, n = 5 in each group)...
July 21, 2016: Folia Histochemica et Cytobiologica
Anna Kozlowska, Przemyslaw Kwiatkowski, Agnieszka Oponowicz, Mariusz Majewski, Zbigniew Kmiec, Janusz Godlewski
INTRODUCTION: The previously performed studies showed that the presence of colorectal cancer (CRC) tumor is associated with the atrophy of myenteric plexuses in the vicinity of cancer invasion; however, the possible mechanisms of this phenomenon are not known. The aim of the present study was to determine whether the atrophic changes of the enteric nervous system (ENS) within an intestine wall of the CRC patients were caused by apoptosis or necrosis and whether they were associated with changes in the number of galanin-immunoreactive (GAL-Ir) neurons...
July 21, 2016: Folia Histochemica et Cytobiologica
Natalia Rozwadowska, Agnieszka Malcher, Ewa Baumann, Tomasz Jan Kolanowski, Marek Rucinski, Tomasz Miętkiewski, Katarzyna Fiedorowicz, Maciej Kurpisz
INTRODUCTION: Primary cells in vitro culture scale-up is a crucial issue in cell-based tissue and organ regeneration therapy. Reducing costs and space occupied by the cells cultured in vitro has been an important target. Cells cultured in vitro with the use of bioreactor with dextran microcarriers (Cytodex®) have potentially a chance to meet many of the cell therapy requirements. MATERIAL AND METHODS: We used collagen-coated carriers (Cytodex3®) and a spinner flask bioreactor to develop environment suitable for human myoblast proliferation...
June 8, 2016: Folia Histochemica et Cytobiologica
Piotr Eder, Liliana Lykowska-Szuber, Katarzyna Iwanik, Iwona Krela-Kazmierczak, Kamila Stawczyk-Eder, Przemyslaw Majewski, Krzysztof Linke, Elaine W Kay, Aldona Wozniak
INTRODUCTION: Immune-mediated angiogenesis may play an important role in the pathogenesis of inflammatory lesions in Crohn's disease (CD). The study aimed to assess the influence of anti-tumour necrosis factor (anti-TNF) therapy on the angiogenesis in relation to microscopic and endoscopic healing in CD patients. MATERIAL AND METHODS: Colonic tissue samples from 17 CD patients were taken during colonoscopy before and after anti-TNF therapy. Endoscopic and microscopic severities were estimated using validated scores...
June 8, 2016: Folia Histochemica et Cytobiologica
Pawel Nowak, Paulina Paluchowska
Acinetobacter baumannii is a Gram-negative, glucose-non-fermenting, oxidase-negative coccobacillus, most commonly associated with the hospital settings. The ability to survive in adverse environmental conditions as well as high level of natural and acquired antimicrobial resistance make A. baumannii one of the most important nosocomial pathogens. While carbapenems have long been considered as antimicrobials of last-resort, the rates of clinical A. baumannii strains resistant to these antibiotics are increasing worldwide...
June 8, 2016: Folia Histochemica et Cytobiologica
Bartlomiej Perek, Agnieszka Malinska, Jerzy Gasowski, Danuta Ostalska-Nowicka, Anna Perek, Marek Jemielity, Maciej Zabel, Michal Nowicki
INTRODUCTION: Currently, elderly people constitute a large proportion of patients undergoing coronary artery bypass grafting (CABG). Activated smooth muscle cells in the tunica media of saphenous vein (SV) grafts are thought to play a key role in the formation of neointima and development of occluding atherosclerotic plaques. The aim of this study was to identify ageing-related variations in the expression of the smooth muscle cells pro-teins that may impact on patency rate of the grafts and the CABG outcomes...
2016: Folia Histochemica et Cytobiologica
Shaoping Huang, Jing Wang, Lei Wang
INTRODUCTION: Sucla2, a β subunit of succinyl coenzyme A synthase, is located in the mitochondrial matrix. Sucla2 catalyzes the reversible synthesis of succinate and adenosine triphosphate (ATP) in the tricarboxylic acid cycle. Sucla2 expression was found to be correlated with the capacitation of boar spermatozoa. We have previously reported that Sucla2 was decreased in the testes of rats with spermatogenesis failure after exposure to endocrine disruptor BDE47. Yet, the expression model of Sucla2 in spermatogenesis and the function of Sucla2 in spermatogenic cells are still unclear...
2016: Folia Histochemica et Cytobiologica
Zofia Bielanska-Osuchowska, Barbara Bilinska, Maria Chosia, Jerzy Kawiak, Andrzej Lukaszyk
No abstract text is available yet for this article.
2016: Folia Histochemica et Cytobiologica
Anna Osiecka-Iwan, Stanislaw Moskalewski, Anna Hyc
INTRODUCTION: We have previously observed that rat synovial membranes incubated in medium containing cartilage interstitial fluid (CIF) responded by changes in the expression of hyaluronan synthases (HAS1 and HAS2), collagen type I, versican, aggrecan, lubricin, matrix metalloproteinases 2 and 3 (MMP2 and MMP3), tissue inhibitors of metalloproteinases (TIMP1, 2 and 3), transforming growth factor β1 (TGFβ1), tumor necrosis factor (TNF), interleukin (IL) 1β and IL-6. The aim of the study was to evaluate the influence of particular cytokines found in CIF on the gene expression of extracellular matrix (ECM) proteins in synovial membrane cells...
2016: Folia Histochemica et Cytobiologica
Anna Kotulak, Agata Wronska, Jaroslaw Kobiela, Janusz Godlewski, Marcin Stanislawowski, Piotr Wierzbicki
INTRODUCTION: The colorectal cancer (CRC) is one of the most frequent cancer in Poland and worldwide. This disease is characterized by distinct genetic alterations. p73 belongs to the p53 gene family; however, its role in the pathogenesis of CRC has not been completely understood. p73 gene encodes several mRNA variants and protein isoforms with its longest and fully functional p73a (mRNA) and TAp73a (protein) isoform. The aim of the study was to investigate p73 gene expression at the mRNA (p73a) and protein (TAp73a) levels in CRC...
2016: Folia Histochemica et Cytobiologica
Serap Uslu, Arzu Gercek Irban, Arel Gereli, Elif Ilgaz Aydinlar, Pinar Elpen, Umit Ince
INTRODUCTION: Many patients of all ages are admitted to hospital due to bone fractures. The etiology of fracture has a very wide spectrum, ranging from motor accidents to pathological conditions such as tumors, osteoporosis, and others. Bone fracture healing is a well-programmed and well-organized process, but is also long and intractable. The outcome of this process is therefore affected by many factors, such as the patient's age, ethnicity, nutritional status, and extent of the fracture...
2016: Folia Histochemica et Cytobiologica
Piotr Donizy, Maciej Kaczorowski, Agnieszka Halon, Marek Leskiewicz, Rafal Matkowski
INTRODUCTION: Lymphatic invasion (LYI) and lymphangiogenesis in the primary tumor are important processes related to the dissemination of neoplasms. The aim of the study was to examine the relationship of LYI status in cutaneous melanoma with patient survival and clinicopathological data. MATERIAL AND METHODS: LYI status was assessed in 104 hematoxylin-eosin (H&E) stained melanoma primary tumor samples and analyzed in relation to patient survival and other clinicopathological and histopathological characteristics...
2016: Folia Histochemica et Cytobiologica
Haidong Wang, Linli Xue, Yanan Li, Bingling Zhao, Tianzhi Chen, Ying Liu, Lucheng Chang, Juan Wang
INTRODUCTION: To investigate whether the membrane-associated transporter protein SLC45A2 is differentially expressed in the skin of sheep with different coat colors and to determine its correlation with coat color establishment in sheep. MATERIAL AND METHODS: The expression of SLC45A2 in sheep skin samples with different coat colors was qualitatively and quantitatively analyzed by PCR amplification, RT-PCR, immunohistochemical staining and Western blotting. RESULTS: A 193-bp SLC45A2 CDS sequence was successfully amplified from sheep skin samples with diverse coat colors...
2016: Folia Histochemica et Cytobiologica
Maria Jaworska-Wilczynska, Pawel Trzaskoma, Andrzej A Szczepankiewicz, Tomasz Hryniewiecki
Normal pericardium consists of an outer sac called fibrous pericardium and an inner one called serous pericardium. The two layers of serous pericardium: visceral and parietal are separated by the pericardial cavity, which contains 20 to 60 mL of the plasma ultrafiltrate. The pericardium acts as mechanical protection for the heart and big vessels, and a lubrication to reduce friction between the heart and the surrounding structures. A very important role in all aspects of pericardial functions is played by mesothelial cells...
2016: Folia Histochemica et Cytobiologica
Zbigniew Kmiec
No abstract text is available yet for this article.
2016: Folia Histochemica et Cytobiologica
Izabela Michalczyk, Monika Toporkiewicz, Patrycja M Dubielecka, Anna Chorzalska, Aleksander F Sikorski
INTRODUCTION: During studies on chemotherapy-induced apoptosis in lymphoid cells, we noted that aggregation of spectrin occurred early in apoptosis, i.e. before activation of initiator caspase(s) and prior to exposure of phosphatidylserine (PS). We also found that protein kinase C theta (PKC-θ) co-localized with spectrin in these aggregates. Our previously published studies indicated that in formation of early apoptotic spectrin aggregates, either PKC-θ or other apoptosis-related proteins are involved...
2016: Folia Histochemica et Cytobiologica
Joanna Kosalka, Bogdan Jakiela, Jacek Musial
INTRODUCTION: Renal involvement in systemic lupus erythematosus (SLE) is associated with production of antibodies to double stranded DNA, deposition of immune complexes and organ damage. These processes have been linked with abnormalities in B- and T-cell memory compartments. The aim of the study was to analyze subsets of peripheral memory B-cells and T-cells in lupus nephritis (LN) patients. MATERIAL AND METHODS: We used multicolor flow cytometry to analyze major memory subsets of peripheral blood B-cells (defined by CD27, IgD and CD21) and T-cells (CD45RA, CD45RO, CCR7) in 32 patients with active or inactive LN, and 23 control subjects...
2016: Folia Histochemica et Cytobiologica
Salime Pelin Ertürküner, Elif Yaprak Saraç, Semil Selcen Göçmez, Hakan Ekmekçi, Zeynep Banu Öztürk, İsmail Seçkin, Özkan Sever, Kadircan Keskinbora
INTRODUCTION: Experimental animal models of acute uveitis, an inflammatory eye disease, can be established via endotoxin-induced inflammation. Propolis, a natural substance collected by honeybees from buds and tree exudates, has antioxidant, antibacterial, antiviral, and anti-inflammatory effects. We investigated the effects of propolis, obtained from the Sakarya province of Turkey, on endotoxin-induced uveitis using immunohistochemical, ultrastructural, and biochemical approaches. MATERIAL AND METHODS: Male Wistar albino rats (n = 6/group) received intraperitoneal (ip) lipopolysaccharide (LPS) endotoxin (150 μg/kg) followed by aqueous extract of propolis (50 mg/kg ip) or vehicle; two additional groups received either saline (control) or propolis only...
2016: Folia Histochemica et Cytobiologica
Xiaoyuan Xu, Xingnuan Li, Ruiqiao Yan, He Jiang, Tao Wang, Lili Fan, Jianfang Wu, Jun Cao, Weidong Li
INTRODUCTION: Adipogenesis comprises multiple processes by which mesenchymal stem cells differentiate into adipocytes. To increase our knowledge of the mechanism underlying adipogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs), we performed full-genome gene expression microarray and gene ontology analyses of induced differentiation of hMSCs. MATERIAL AND METHODS: Adipogenic differentiation of hMSCs was induced by an adipogenic medium, and total RNA was extracted from undifferentiated hMSCs (day 0) and differentiated adipocytes (day 14)...
2016: Folia Histochemica et Cytobiologica
Malgorzata Radzimirska, Krystyna Bogus-Nowakowska, Tadeusz Kuder, Anna Robak
INTRODUCTION: Cocaine- and amphetamine-regulated transcript (CART), neuropeptide Y (NPY) and galanin (GAL) act as neurotransmitters and neuromodulators in both the central and peripheral nervous systems. Their presence has been found in different taxonomic groups, in particular in mammals. However, only few investigators have studied these neuropeptides in the class Aves (birds). The aim of the present study was to describe the distribution of CART, NPY and GAL in the pterygopalatine ganglion (PPG) of the domestic duck (Anas platyrhynchos f...
2016: Folia Histochemica et Cytobiologica
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