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International Journal of Food Microbiology

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https://www.readbyqxmd.com/read/28910678/colorimetric-aptasensor-for-the-detection-of-salmonella-enterica-serovar-typhimurium-using-znfe2o4-reduced-graphene-oxide-nanostructures-as-an-effective-peroxidase-mimetics
#1
Shijia Wu, Nuo Duan, Yueting Qiu, Jinghong Li, Zhouping Wang
A new colorimetric aptasensor platform was fabricated to detect Salmonella enterica serovar typhimurium based on the peroxidase-like activity of ZnFe2O4-reduced graphene oxide (ZnFe2O4/rGO) nanostructures. The synthesized ZnFe2O4/rGO can catalytically oxidize 3,3',5,5'-tetramethylbenzidine (TMB) by H2O2 and generate a typical blue product, which was detected by micro-reader at 652nm. Therefore, ZnFe2O4/rGO was conjugated with aptamer to act as the signal probe. Biotin modified aptamer was immobilized onto the micro-plate to act as the capture probe...
September 6, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28892763/passion-fruit-by-product-and-fructooligosaccharides-stimulate-the-growth-and-folate-production-by-starter-and-probiotic-cultures-in-fermented-soymilk
#2
Marcela Albuquerque Cavalcanti Albuquerque, Raquel Bedani, Jean Guy LeBlanc, Susana Marta Isay Saad
Two starter cultures (Streptococcus (St.) thermophilus ST-M6 and TA-40) and five probiotic strains (St. thermophilus TH-4, Lactobacillus (Lb.) acidophilus LA-5, Lb. rhamnosus LGG, Lb. fermentum PCC, and Lb. reuteri RC-14) were used to ferment different soymilk formulations supplemented with passion fruit by-product and/or fructo-oligosaccharides (FOS) with the aim of increasing folate concentrations. Growth and folate production of individual strains were evaluated and the results used to select co-cultures...
September 5, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28889054/evaluation-of-chemical-immersion-treatments-to-reduce-microbial-populations-in-fresh-beef
#3
Ahmed Kassem, Joseph Meade, James Gibbons, Kevina McGill, Ciara Walsh, James Lyng, Paul Whyte
The aim of the current study was to assess the ability of a number of chemicals (acetic Acid (AA), citric acid (CA) lactic acid (LA), sodium decanoate (SD) and trisodium phosphate (TSP)) to reduce microbial populations (total viable count, Campylobacter jejuni, Escherichia coli, Salmonella typhimurium and Listeria monocytogenes) on raw beef using an immersion system. The following concentrations of each chemical were used: 3 & 5% for AA, CA, LA, SD and 10 &12% for TSP. Possible synergistic effects of using combinations of two chemicals sequentially (LA+CA and LA+AA) were also investigated...
September 5, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28889055/cells-qpcr-as-a-direct-quantitative-pcr-method-to-avoid-microbial-dna-extractions-in-grape-musts-and-wines
#4
Verónica Soares-Santos, Isabel Pardo, Sergi Ferrer
A novel quantitative PCR assay called Cells-qPCR has been developed for the rapid detection and quantification of yeasts, lactic acid bacteria (LAB) and acetic acid bacteria (AAB) directly from grape must and wine that does not require DNA extraction. The assay was tested on Brettanomyces bruxellensis, Saccharomyces cerevisiae, Lactobacillus plantarum, Oenococcus oeni, Acetobacter aceti and Gluconobacter oxydans in culture media, and in white and red grape musts and wines. Standard curves were constructed from DNA and cells for the six target species in all the matrices...
September 4, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28881263/metagenetic-analysis-of-the-bacterial-communities-of-edible-insects-from-diverse-production-cycles-at-industrial-rearing-companies
#5
D Vandeweyer, S Crauwels, B Lievens, L Van Campenhout
Despite the continuing development of new insect-derived food products, microbial research on edible insects and insect-based foods is still very limited. The goal of this study was to increase the knowledge on the microbial quality of edible insects by comparing the bacterial community composition of mealworms (Tenebrio molitor) and crickets (Acheta domesticus and Gryllodes sigillatus) from several production cycles and rearing companies. Remarkable differences in the bacterial community composition were found between different mealworm rearing companies and mealworm production cycles from the same company...
August 24, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28850852/occurrence-and-growth-of-listeria-monocytogenes-in-packaged-raw-milk
#6
Hanna Castro, Marjo Ruusunen, Miia Lindström
The increased availability of packaged raw drinking milk necessitates the investigation of the occurrence and growth of Listeria monocytogenes in raw milk during distribution and storage. The occurrence of L. monocytogenes in 105 retailed raw milk bottles, 115 bulk tank milk samples, 23 in-line milk filter socks and in 50 environmental samples collected from an on-farm dairy establishment were investigated. Growth of inoculated low-level L. monocytogenes contamination was also investigated in two types of raw milk packaging, namely in 1-litre plastic bottles and 3-litre bag-in-boxes, both stored at three different storage temperatures of 6, 8 and 10°C...
August 23, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28915412/aspergillus-section-flavi-community-structure-in-zambia-influences-aflatoxin-contamination-of-maize-and-groundnut
#7
Paul W Kachapulula, Juliet Akello, Ranajit Bandyopadhyay, Peter J Cotty
Aflatoxins are cancer-causing, immuno-suppressive mycotoxins that frequently contaminate important staples in Zambia including maize and groundnut. Several species within Aspergillus section Flavi have been implicated as causal agents of aflatoxin contamination in Africa. However, Aspergillus populations associated with aflatoxin contamination in Zambia have not been adequately detailed. Most of Zambia's arable land is non-cultivated and Aspergillus communities in crops may originate in non-cultivated soil...
August 19, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28843123/antimicrobial-efficacy-of-cinnamomum-javanicum-plant-extract-against-listeria-monocytogenes-and-its-application-potential-with-smoked-salmon
#8
Wenqian Yuan, Hui Wen Lee, Hyun-Gyun Yuk
Extracts from medicinal plants have been reported to possess good antimicrobial properties, but a majority of them remain unexplored. This study aimed at identifying a novel plant extract with antimicrobial activity, to validate its efficacy in food model, and to elucidate its composition and antimicrobial mechanism. A total of 125 plant extracts were screened, and Cinnamomum javanicum leaf and stem extract showed potential antimicrobial activity against Listeria monocytogenes (MIC=0.13mg/mL). Total phenolic content of the extract was 78...
August 19, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28843121/exploring-the-microbial-origins-of-p-cresol-and-its-co-occurrence-pattern-in-the-chinese-liquor-making-process
#9
Hai Du, Bo Liu, Xueshan Wang, Yan Xu
The compound p-cresol is the major off-odor and toxic component of strong aroma-type Chinese liquor. To trace its origin, the p-cresol contents in the liquor-making process were detected by gas chromatography-mass spectrometry. The prokaryotic communities involved in the process were revealed by 16S rRNA gene MiSeq sequencing. The results showed that the microbial diversity and concentration of p-cresol in pit mud significantly increased with increased pit depth. Canonical correspondence analysis further revealed that p-cresol was positively correlated with the genera Aminobacterium, Clostridium, Sedimentibacter and Syntrophomonas...
August 19, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28843122/lactobionic-acid-enhances-the-synergistic-effect-of-nisin-and-thymol-against-listeria-monocytogenes-scott-a-in-tryptic-soy-broth-and-milk
#10
Huaiqiong Chen, Qixin Zhong
Listeria monocytogenes is a Gram-positive opportunistic human pathogen and it remains a significant cause of foodborne illnesses. A variety of natural and synthetic compounds have been studied to inhibit the growth of L. monocytogenes in foods. Antimicrobial combinations with synergistic antilisterial properties can reduce the dose of each antimicrobial, which can be further enhanced by chelating compounds. Therefore, the objective of this study was to determine antilisterial properties of binary or ternary combinations of lactobionic acid (LBA), nisin, and thymol in tryptic soy broth (TSB), 2% reduced-fat milk, and whole milk...
August 18, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28889001/decontamination-of-fresh-cut-cucumber-slices-by-a-combination-of-a-modified-chitosan-coating-containing-carvacrol-nanoemulsions-and-pulsed-light
#11
Özge Taştan, Gianpiero Pataro, Francesco Donsì, Giovanna Ferrari, Taner Baysal
In this study, the impact of the combination of pulsed light (PL) treatments with antimicrobial coatings, consisting of modified chitosan suspensions incorporating carvacrol nanoemulsions, was investigated on the decontamination of fresh-cut cucumber slices. The upper surface of the cucumber slices, with or without the coating deposition, was inoculated with Escherichia coli ATCC 26 to reach a final concentration of 10(7)CFU/g of the vegetable. PL treatments were conducted at different fluence (4, 8, and 12J/cm(2)) on the inoculated surface of cucumber slices...
August 16, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28843125/combination-of-essential-oil-compounds-and-phenolic-acids-against-escherichia-coli-o157-h7-in-vitro-and-in-dry-fermented-sausage-production
#12
Natan V B Meira, Richard A Holley, Keliani Bordin, Renata E F de Macedo, Fernando B Luciano
Escherichia coli O157:H7 is a foodborne pathogen that causes hemorrhagic colitis and hemolytic uremic syndrome. The low dose of infection and severity of the disease represent a concern to public health. Natural compounds have been widely applied as food additives to replace synthetic preservatives. The aim of this study was to determine the efficiency of essential oil compounds (EOCs) in combination with phenolic acids (PA) in vitro and in dry-fermented sausage production. Minimum Inhibitory Concentration (MIC) and Fractional Inhibitory Concentration index (FICindex) were determined for a 5-strain mixture of E...
August 16, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28843124/impact-of-the-reusing-of-food-manufacturing-wastewater-for-irrigation-in-a-closed-system-on-the-microbiological-quality-of-the-food-crops
#13
Luciano Beneduce, Giuseppe Gatta, Antonio Bevilacqua, Angela Libutti, Emanuele Tarantino, Micol Bellucci, Eleonora Troiano, Giuseppe Spano
In order to evaluate if the reuse of food industry treated wastewater is compatible for irrigation of food crops, without increased health risk, in the present study a cropping system, in which ground water and treated wastewater were used for irrigation of tomato and broccoli, during consecutive crop seasons was monitored. Water, crop environment and final products were monitored for microbial indicators and pathogenic bacteria, by conventional and molecular methods. The microbial quality of the irrigation waters influenced sporadically the presence of microbial indicators in soil...
August 16, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28843120/evaluation-of-pulsed-light-treatments-on-inactivation-of-salmonella-on-blueberries-and-its-impact-on-shelf-life-and-quality-attributes
#14
Xinang Cao, Runze Huang, Haiqiang Chen
Blueberry have a short shelf life when fully ripe and susceptible to contamination of various pathogens. Our study investigated the effect of pulsed light (PL) on inactivation of Salmonella on blueberries and its impact on shelf-life, quality attributes and health-benefit compounds of blueberries. Dry PL (6J/cm(2)) and water-assisted PL (samples were agitated in water during PL treatment; 9J/cm(2)) along with two controls, dry control (untreated) and water-assisted control (water washing without PL), were applied to blueberries with subsequent storages at room temperature (3days) or 5°C (7days)...
August 16, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28843119/biofilm-of-escherichia-coli-o157-h7-on-cantaloupe-surface-is-resistant-to-lauroyl-arginate-ethyl-and-sodium-hypochlorite
#15
Yezhi Fu, Amanda J Deering, Arun K Bhunia, Yuan Yao
Biofilms formed by Escherichia coli O157:H7 on cantaloupe rind were characterized in this study. Cantaloupe rind pieces inoculated with E. coli O157:H7 B6-914 was sampled after 2, 12, and 24h incubation for imaging with cryo-scanning electron microscopy (Cryo-SEM) or treating with lauroyl arginate ethyl (LAE) or sodium hypochlorite (SHC). Cryo-SEM images showed that E. coli O157:H7 formed a biofilm within 12h on the rind surface. For rind samples treated with LAE or SHC, the residual cell counts were significantly different (p<0...
August 16, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28843118/antimicrobial-evaluation-of-novel-poly-lactic-acid-based-nanocomposites-incorporated-with-bioactive-compounds-in-vitro-and-in-refrigerated-vacuum-packed-cooked-sausages
#16
Mohammadreza Rezaeigolestani, Ali Misaghi, Ali Khanjari, Afshin Akhondzadeh Basti, Ali Abdulkhani, Samira Fayazfar
Biodegradability and antimicrobial activity of food packaging materials are among the most attractive parameters in modern food industries. In order to develop biodegradable poly-lactic acid (PLA) film to antibacterial nanocomposites, different concentration of Zataria multiflora Bioss. essential oil (ZME), propolis ethanolic extract (PEE) and cellulose nanofiber (CNF) were incorporated to the polymer by solvent casting method. The resulting films were characterized by mechanical and physical tests and their antimicrobial application was evaluated in-vitro against four common foodborne pathogens and in vacuum-packed cooked sausages during refrigerated storage...
August 16, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28888104/effects-of-ultraviolet-light-emitting-diodes-leds-on-microbial-and-enzyme-inactivation-of-apple-juice
#17
Merve Pelvan Akgün, Sevcan Ünlütürk
In this study, the effects of Ultraviolet light-emitting diodes (UV-LEDs) on the inactivation of E. coli K12 (ATCC 25253), an indicator organism of E. coli O157:H7, and polyphneoloxidase (PPO) in cloudy apple juice (CAJ) were investigated. The clear (AJ) and cloudy apple juice were exposed to UV rays for 40min by using a UV device composed of four UV-LEDs with peak emissions at 254 and 280nm and coupled emissions as follows: 254/365, 254/405, 280/365, 280/405 and 254/280/365/405nm. UV-LEDs at 254nm achieved 1...
August 15, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28841447/variation-of-genomic-islands-and-flanking-fragments-in-vibrio-parahaemolyticus-isolates-from-environmental-and-clinical-sources-in-taiwan
#18
Po-Shen Chi, Hin-Chung Wong
Vibrio parahaemolyticus is a halophilic foodborne pathogenic bacterium that causes gastroenteritis; it has become an issue of global concern since the emergence and spread of pandemic O3:K6 strains. This study evaluated the role of Vibrio pathogenicity island (VPaI)-associated fragments in the genetic variation and grouping of this pathogen. Distribution of some VPaI fragments and flanking fragments (VPaI-1, VPaI-4, VPaI-5, VPaI-6 and VPaI-7) was determined in a total of 53 V. parahaemolyticus isolates from environmental and clinical sources in Taiwan, and supported by the sequences of seven fragments of VPaI-4 and its flanking fragment VP2145...
August 9, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28822890/production-of-a-novel-monoclonal-antibody-applicable-for-an-immunochromatographic-assay-for-kudoa-septempunctata-spores-contaminating-the-raw-olive-flounder-paralichthys-olivaceus
#19
Michio Jinnai, Takao Kawai, Tetsuya Harada, Yasutaka Nishiyama, Hiroshi Yokoyama, Sho Shirakashi, Hiroshi Sato, Junko Sakata, Yuko Kumeda, Yutaka Fukuda, Kikuyo Ogata, Kentaro Kawatsu
Kudoa septempunctata, a myxosporean parasite of the olive flounder (Paralichthys olivaceus), causes foodborne gastroenteritis after ingestion of contaminated raw flounder. Available methods to detect K. septempunctata require expensive equipment, well-trained personnel, and lengthy procedures. Here we generated a novel monoclonal antibody (MAb 15G11) against K. septempunctata and used it to produce a prototype immunochromatographic assay (prototype Kudoa-ICA). Within 15min, the prototype Kudoa-ICA detected ≥1...
August 9, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28803156/sequential-transition-of-the-injury-phenotype-temperature-dependent-survival-and-transcriptional-response-in-listeria-monocytogenes-following-lethal-h2o2-exposure
#20
Yoshitsugu Ochiai, Fumiya Yamada, Yuko Yoshikawa, Mariko Mochizuki, Takashi Takano, Ryo Hondo, Fukiko Ueda
The food-borne pathogen Listeria monocytogenes is present persistently in food processing environments, where this bacterium is exposed to various stress factors, including oxidative stress. This study aimed to elucidate the temperature-dependent response of L. monocytogenes to H2O2 exposure and the phenotypic changes in colony formation by H2O2-treated bacteria. Survival curves indicated an increase in the resistance to H2O2 in L. monocytogenes as the temperature decreased during the stress exposure procedure...
August 3, 2017: International Journal of Food Microbiology
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