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Journal of Biotechnology

Erzheng Su, Yang Meng, Chenxi Ning, Xiaoqiang Ma, Senwen Deng
Magnetic Fe 3 O 4 nanoparticles were prepared and embedded into the Combi-CLEAs to produce the magnetic Combi-CLEAs in this work. The process for magnetic Combi-CLEAs preparation was optimized, and its properties were investigated. The optimum temperature, thermal stability and optimum pH of magnetic Combi-CLEAs were similar to those of Combi-CLEAs. The catalytic performance of magnetic Combi-CLEAs was tested with the biosynthesis of (S)-ethyl 4-chloro-3-hydroxybutyrate ((S)-CHBE). Magnetic Combi-CLEAs could tolerate higher substrate concentration in the biphasic system...
February 13, 2018: Journal of Biotechnology
Inhyuk Yeo, Yong-Jik Lee, Kyeongseop Song, Hyeon-Su Jin, Jae-Eun Lee, Dajeong Kim, Dong-Woo Lee, Nam Joo Kang
Bioactive peptides contribute to various cellular processes including improved skin physiology. Hence, bioactive keratins have attracted considerable attention as active cosmetic ingredients for skin health. Here, we obtained low molecular weight (LMW) keratins from native chicken feathers by anaerobic digestion with an extremely thermophilic bacterium Fervidobacterium islandicum AW-1, followed by stepwise fractionation through ultrafiltration. To assess the effects of the feather keratins on skin health, we performed in vitro and ex vivo assays to investigate their inhibitory effects on matrix metalloproteinases (MMPs)...
February 10, 2018: Journal of Biotechnology
Anja Sophie Schmid, Diana Tintor, Dario Neri
Neutrophils can strongly influence disease activity in cancer and in chronic inflammation. Here, we report for the first time the construction and characterization of antibody-fusion proteins featuring granulocyte-colony stimulating factor and interleukin-3 as payloads capable of enhancing neutrophil activity and a novel antibody-interleukin-4 fusion protein with neutrophil inhibitory potential. We used the F8 antibody specific to the alternatively-spliced extra domain A (EDA) of fibronectin as a targeting agent, since the cognate antigen is strongly upregulated in diseases characterized by angiogenesis...
February 10, 2018: Journal of Biotechnology
Tiantian Wang, Dongshu Wang, Yufei Lyu, Erling Feng, Zhu Li, Chunjie Liu, Yanchun Wang, Xiankai Liu, Hengliang Wang
To investigate gene function in Bacillus anthracis, a high-efficiency cloning system is required with an increased rate of allelic exchange. Golden Gate cloning is a molecular cloning strategy allowing researchers to simultaneously and directionally assemble multiple DNA fragments to construct target plasmids using type IIs restriction enzymes and T4 DNA ligase in the same reaction system. Here, a B. anthracis S-layer protein EA1 allelic exchange vector was successfully constructed using the Golden Gate method...
February 10, 2018: Journal of Biotechnology
Krisztina Koczka, Philipp Peters, Wolfgang Ernst, Heinz Himmelbauer, Lisa Nika, Reingard Grabherr
The baculovirus insect cell expression system has become a firmly established production platform in biotechnology. Various complex proteins, multi-subunit particles including veterinary and human vaccines are manufactured with this system on a commercial scale. Apart from baculovirus infected Spodoptera frugiperda (Sf9) cells, the Trichoplusia ni (HighFive) cell line is alternatively used as host organism. In this study, we explored the protein production capabilities of Tnms42 insect cells, a new derivative of HighFive, which is free of latent nodavirus infection...
February 9, 2018: Journal of Biotechnology
Valentin Sóti, Silvia Lenaerts, Iris Cornet
Enzyme cost is considered to be one of the most significant factors defining the final product price in lignocellulose hydrolysis and fermentation. Enzyme immobilization and recycling can be a tool to decrease costs. However, high solid loading is a key factor towards high product titers, and recovery of immobilized enzymes from this thick liquid is often overlooked. This paper aims to evaluate the economic feasibility of immobilized enzymes in simultaneous saccharification and fermentation (SSF) of lignocellulose biomass in general, as well as the recuperation of magnetic immobilized enzymes (m-CLEAs) during high solid loading in simultaneous saccharification, detoxification and fermentation processes (SSDF) of lignocellulose biomass...
February 8, 2018: Journal of Biotechnology
Taiza da Cunha Soares, Carliane Rebeca Coelho da Silva, Julita Maria Frota Chagas Carvalho, José Jaime Vasconcelos Cavalcanti, Liziane Maria de Lima, Péricles de Albuquerque Melo Filho, Liv Soares Severino, Roseane Cavalcanti Dos Santos
Substantial progress is being reported in the techniques for plant transformation, but successful regeneration of some genotypes remains a challenging step in the attempts to transform some recalcitrant species. GhSERK1 gene is involved on embryo formation, and its overexpression enhances the embryogenic competence. In this study we validate a short GhSERK1 probe in order to identify embryogenic cotton genotypes using RT-qPCR and blotting assays. Cotton genotypes with contrasting somatic embryogenic capacity were tested using in vitro procedures...
February 7, 2018: Journal of Biotechnology
Tobias May, Daniela Polag, Frank Keppler, Markus Greule, Liane Müller, Helmut König
A broad methanotrophic community consisting of 16 different operational taxonomic units (OTUs) was detected by particulate methane monooxygenase A (pmoA) gene analyses of reactor sludge samples obtained from an industrial biogas plant. Using a cloning-sequencing approach, 75% of the OTUs were affiliated to the group of type I methanotrophs (γ-Proteobacteria) and 25 % to type II methanotrophs (α-Proteobacteria) with a distinct predominance of the genus Methylobacter. By database matching, half of the total OTUs may constitute entirely novel species...
February 3, 2018: Journal of Biotechnology
Myung Hwan Lee, Raees Khan, Weixin Tao, Kihyuck Choi, Seung Yeup Lee, Jae Wook Lee, Eul Chul Hwang, Seon-Woo Lee
Autoinducers are indispensable for bacterial cell-cell communication. However, due to the reliance on culture-based techniques, few autoinducer-hydrolyzing enzymes are known. In this study, we characterized soil metagenome-derived unique enzymes capable of hydrolyzing 3-hydroxypalmitic acid methyl ester (3-OH PAME), an autoinducer of the plant pathogenic bacterium Ralstonia solanacearum. Among 146 candidate lipolytic clones from a soil metagenome library, 4 unique enzymes capable of hydrolyzing the autoinducer 3-OH PAME, termed ELP86, ELP96, ELP104, and EstDL33, were selected and characterized...
February 3, 2018: Journal of Biotechnology
Esther Frohnmeyer, Farina Frisch, Sven Falke, Christian Betzel, Markus Fischer
Aptamers are single-stranded DNA or RNA oligonucleotides, which have been emerging as recognition elements in disease diagnostics and food control, including the detection of bacterial toxins. In this study, we employed the semi-automated just in time-selection to identify aptamers that bind to cholera toxin (CT) with high affinity and specificity. CT is the main virulence factor of Vibrio cholerae and the causative agent of the eponymous disease. For the selected aptamers, dissociation constants in the low nanomolar range (23-56 nM) were determined by fluorescence-based affinity chromatography and cross-reactivity against related proteins was evaluated by direct enzyme-linked aptamer assay (ELAA)...
February 2, 2018: Journal of Biotechnology
Sidharth Arora, Richa Rani, Sanjoy Ghosh
In recent years, substantial credibility in employing Solid-State Fermentation (SSF) technique has been witnessed owing to its numerous advantages over submerged fermentation (SmF). In spite of enormous advantages, true potential of SSF technology has not been fully realized at industrial scale. The lack of rational and scalable bioreactor designs backed by mathematical models and automated control system that could successfully address heterogeneity with respect to heat and mass, and also operate aseptically, remains the prime reason for it...
February 1, 2018: Journal of Biotechnology
Stefano Spertino, Lara Boatti, Sara Icardi, Marcello Manfredi, Chiara Cattaneo, Emilio Marengo, Maria Cavaletto
Lignocellulose degradation is a challenging step for value added products and biofuels production. Cellulomonas fimi secretes complex mixtures of carbohydrate active enzymes (CAZymes) which synergistically degrade cellulose and hemicelluloses. Their characterization may provide new insights for enzymatic cocktails implementation. Bioinformatic analysis highlighted 1127 secreted proteins, constituting the in silico secretome, graphically represented in a 2DE map. According to Blast2GO functional annotation, many of these are involved in carbohydrates metabolism...
January 30, 2018: Journal of Biotechnology
Eun Ju Yun, Sora Yu, Sooah Kim, Kyoung Heon Kim
Marine red macroalgae have received much attention as a sustainable resource for producing bio-based products. Therefore, understanding the metabolic pathways of carbohydrates from red macroalgae, in fermentative microorganisms, is crucial for efficient bioconversion of the carbohydrates into bio-based products. Recently, the novel catabolic pathway of 3,6-anhydro-l-galactose (AHG), the main component of red macroalgae, was discovered in a marine bacterium, Vibrio sp. strain EJY3. However, the global metabolic network in response to AHG remains unclear...
January 30, 2018: Journal of Biotechnology
Anjuman Ara Bhuyan, Atta Muhammad Memon, Ali Akbar Bhuiyan, Li Zhonghua, Bingzhou Zhang, Shiyi Ye, Li Mengying, Qi-Gai He
Bovine viral diarrhea virus (BVDV) is the etiological agent of BVD causes substantial economic losses and endemic in world-wide cattle population. Mucosal immunity plays an important role in protection against BVDV infection and Lactobacillus casei is believed as an excellent live vaccine vector for expressing foreign genes. In this study, we have constructed a novel recombinant L. casei/pELX1-E2 strain expressing the most immunogenic E2 antigen of BVDV; using growth phage dependent surface expression system pELX1...
January 30, 2018: Journal of Biotechnology
Na Liu, Shuzhen Ni, Arthur J Ragauskas, Xianzhi Meng, Naijia Hao, Yingjuan Fu
An effective method to functionalize chitosan with 4-hexyloxyphenol (HP) under homogeneous reaction conditions was developed using laccase as the catalyst. The resulting copolymer was characterized for chemical structure, grafted-HP content, surface morphology, thermal stability, antioxidant capacity, hydrophobic properties and tensile strength. Solid-state 13C NMR spectrum confirmed the incorporation of HP onto chitosan. X-ray diffraction (XRD) showed a decrease in the degree of crystallinity for laccase/HP treated chitosan compared to pure chitosan...
January 30, 2018: Journal of Biotechnology
In-Seok Yeo, Woo-Yong Shim, Jung Hoe Kim
For the biological production of L-ribulose, conversion by enzymes or resting cells has been investigated. However, expensive or concentrated substrates, an additional purification step to remove borate and the requirement for cell cultivation and harvest steps before utilization of resting cells make the production process complex and unfavorable. Microbial fermentation may help overcome these limitations. In this study, we constructed a genetically engineered Candida tropicalis strain to produce L-ribulose by fermentation with a glucose/L-arabinose mixture...
January 30, 2018: Journal of Biotechnology
Mohsen Fatemi, Nasrin Mollania, Madjid Momeni-Moghaddam, Fatemeh Sadeghifar
Discovery of new properties and special functionalities at the nanoscale materials caused nanotechnology to become one of the leading parts in all sciences namely biology and medicine. Magnetic iron oxide nanoparticles (MIONPs) are among interesting nanomaterials in biomedical arena, which have attracted the attention of many researchers owing to their extensive capabilities. Due to the simple, cost-effective and environmentally-friendly production processes, biosynthesis is of paramount importance between different methods of nanoparticles production...
January 30, 2018: Journal of Biotechnology
Jean Carlos Bettoni, Murilo Dalla Costa, Juliana Aparecida Souza, Gayle M Volk, Osmar Nickel, Fabio Nascimento da Silva, Aike Anneliese Kretzschmar
Apple stem pitting virus (ASPV), Apple chlorotic leaf spot virus (ACLSV) and Apple stem grooving virus (ASGV) are several major viral pathogens of apple trees, responsible for substantial damage to the world's apple industry. This study aimed to evaluate the effectiveness of the encapsulation-dehydration cryopreservation technique to eradicate these viral pathogens from in vitro shoot tips excised from 'Marubakaido' apple rootstock cultures. Axillary shoot tips were excised from in vitro cultures, encapsulated in alginate beads, precultured in MS salts, dehydrated in a laminar flow hood, immersed in liquid nitrogen, then warmed and recovered on medium...
January 29, 2018: Journal of Biotechnology
Kathrin Tomasek, Tobias Bergmiller, Călin C Guet
Buffers are essential for diluting bacterial cultures for flow cytometry analysis in order to study bacterial physiology and gene expression parameters based on fluorescence signals. Using a variety of constitutively expressed fluorescent proteins in Escherichia coli K-12 strain MG1655, we found strong artifactual changes in fluorescence levels after dilution into the commonly used flow cytometry buffer phosphate-buffered saline (PBS) and two other buffer solutions, Tris-HCl and M9 salts. These changes appeared very rapidly after dilution, and were linked to increased membrane permeability and loss in cell viability...
January 17, 2018: Journal of Biotechnology
Mariana B Méndez, Cintia W Rivero, Fernando López-Gallego, José M Guisán, Jorge A Trelles
The 2'-N-deoxyribosyltransferases [NDT; EC] are a group of enzymes widely used as biocatalysts for nucleoside biosynthesis. In this work, the molecular cloning, expression and purification of a novel NDT from Lactobacillus animalis (LaNDT) have been reported. On the other hand, biocatalyst stability has been significantly enhanced by multipoint covalent immobilization using a hetero-functional support activated with nickel-chelates and glyoxyl groups. The immobilized enzyme could be reused for more than 300 h and stored during almost 3 months without activity loss...
January 16, 2018: Journal of Biotechnology
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