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Journal of Biotechnology

Hui Fang, Bing Hu, Yong Nie, Yue-Qin Tang, Xiao-Lei Wu
The type strain Dietzia timorensis ID05-A0528(T), was reported to be able to survive in the highly saline and alkaline environments with diverse carbon sources. In order to more pertinently understand the genetic mechanisms of its environmental tolerance and crude oil emulsification, we reported the complete genome sequence of the strain in the study. The genome contains only one circular chromosome, with the total size of 3,607,892 bps, and the G+C content of this strain is 65.58%, much lower than other type strains of this genus...
October 17, 2016: Journal of Biotechnology
Jinxin Li, Shujie Liu, Juan Wang, Jing Li, Dahui Liu, Jianli Li, Wenyuan Gao
In this work, we selected three fungi strains (Aspergillus niger, Aspergillus flavus and Aspergillus oryzae) as elicitors prepared from mycelium or fermentation broth to improve ginsenosides production in adventitious roots culture. The results showed that ginsenosides production (29.90±4.67mgg(-1)) was significantly enhanced upon elicitation with 200mgL(-1)A. niger elicitor prepared from mycelium, which was 3.52-fold of untreated group. HPLC-ESI-MS(n) analysis was performed, showing that ginsenoside Rb3 was present after treatment with the A...
October 13, 2016: Journal of Biotechnology
Tim de Mooij, Kira Schediwy, René H Wijffels, Marcel Janssen
Under high light conditions, microalgae are oversaturated with light which significantly reduces the light use efficiency. Microalgae with a reduced pigment content, antenna size mutants, have been proposed as a potential solution to increase the light use efficiency. The goal of this study was to investigate the competition between antenna size mutants and wild type microalgae in mass cultures. Using a kinetic model and literature-derived experimental data from wild type Chlorella sorokiniana, the productivity and competition of wild type cells and antenna size mutants were simulated...
October 13, 2016: Journal of Biotechnology
Jifeng Yuan, Pranjul Mishra, Chi Bun Ching
Medium branched-chain esters can be used not only as a biofuel but are also useful chemicals with various industrial applications. The development of economically feasible and environment friendly bio-based fuels requires efficient cell factories capable of producing desired products in high yield. Herein, we sought to use a number of strategies to engineer Saccharomyces cerevisiae for high-level production of branched-chain esters. Mitochondrion-based expression of ATF1 gene in a base strain with an overexpressed valine biosynthetic pathway together with expression of mitochondrion-relocalized α-ketoacid decarboxylase (encoded by ARO10) and alcohol dehydrogenase (encoded by ADH7) not only produced isobutyl acetate, but also 3-methyl-1-butyl acetate and 2-methyl-1-butyl acetate...
October 13, 2016: Journal of Biotechnology
Toshio Mori, Hiroko Kako, Tomoki Sumiya, Hirokazu Kawagishi, Hirofumi Hirai
A lactic acid (LA)-producing strain of the hyper-lignin-degrading fungus Phanerochaete sordida YK-624 with the lactate dehydrogenase-encoding gene from Bifidobacterium longum (Blldh) was constructed. When the endogenous pyruvate decarboxylase gene-knocked down and Blldh-expressing transformant was cultured with beech wood meal, the transformant was able to successively delignify and ferment the substrate. Supplementation of calcium carbonate into the culture medium, significantly increased the level of LA accumulation...
October 13, 2016: Journal of Biotechnology
Mahesh Kudrimoti, Amarintha Curtis, Samar Azawi, Francis Worden, Sanford Katz, Douglas Adkins, Marcelo Bonomi, Jenna Elder, Stephen T Sonis, Richard Straube, Oreola Donini
Dusquetide, a novel Innate Defense Regulator, modulates the innate immune system at a key convergence point in intracellular signaling pathways and has demonstrated activity in both reducing inflammation and increasing clearance of bacterial infection. Innate immunity has also been implicated in the pathogenesis of oral mucositis (OM), a universal toxicity of chemoradiation therapy (CRT). Testing the hypothesis that dusquetide can mitigate the development and duration of OM, preclinical studies have been completed and correlated with interim results from a Phase 2 clinical study in patients undergoing CRT for head and neck cancer...
October 13, 2016: Journal of Biotechnology
Paramasivam Murugan, Lizhu Han, Chee-Yuen Gan, Frans H J Maurer, Kumar Sudesh
Bacterial polyhydroxyalkanoates (PHA) are expensive partly due to the recovery and purification processes. Thus, many studies have been carried out in order to minimize the cost. Here we report on the use of mealworm, which is the larva of mealworm beetle (Tenebrio molitor) to recover PHA granules from Cupriavidus necator. Mealworms were shown to readily consume the freeze-dried C. necator cells and excrete the PHA granules in the form of whitish feces. Further purification using water, detergent and heat resulted in almost 100% pure PHA granules...
October 13, 2016: Journal of Biotechnology
Rui-Heng Yang, Yan Li, Ying Wáng, Jia-Ning Wan, Chen-Li Zhou, Ying Wāng, Ying-Nv Gao, Wen-Jun Mao, Li-Hua Tang, Ming Gong, Ying-Ying Wu, Da-Peng Bao
Pleurotus eryngii (DC.) Quél. is widely used for bioconverting lignocellulosic byproducts into biofuel and value added products. Sequencing and annotating the genome of a monokaryon strain P. eryngii 183 allows us to gain a better understanding of carbohydrate-active enzymes (CAZymes) and oxidoreductases for degradation of lignocellulose in white-rot fungi. The genomic data provides insights into genomic basis of degradation mechanisms of lignin and cellulose and may pave new avenues for lignocellulose bioconversion...
October 11, 2016: Journal of Biotechnology
Weizhu Zeng, Fang Fang, Song Liu, Guocheng Du, Jian Chen, Jingwen Zhou
Yarrowia lipolytica is one of the most intensively investigated α-ketoglutaric acid (α-KG) producers, and metabolic engineering has proven effective for enhancing production. However, regulation of α-KG metabolism remains poorly understood. Genetic engineering of new strains is accompanied by potential safety concerns in some countries and regions. A series of mutants with varied capacity for α-KG production were obtained using random mutagenesis of Y. lipolytica WSH-Z06. Comparative genomics analysis was implemented to identify genes candidates associated with α-KG production...
October 11, 2016: Journal of Biotechnology
Carolina Shene, Yusuf Chisti, Daniela Vergara, César Burgos-Díaz, Mónica Rubilar, Mariela Bustamante
The marine microalga Nannochloropsis oculata is a potential source of eicosapentaenoic acid (EPA, C20:5n3) and carotenoids for use in functional foods and nutraceuticals. Mixotrophic culture of N. oculata using glycerol was examined as a possible way of increasing the biomass and metabolite productivity relative to a pure photoautotrophic culture in modified f/2 medium. The effect of CO2 supply was also tested. EPA production in semi-continuous culture with and without glycerol and CO2 was evaluated. The effects of glycerol supplementation and light/dark cycling on the production of the biomass and EPA are reported for cultures conducted at a constant pH controlled using CO2...
October 8, 2016: Journal of Biotechnology
Maxime Toussaint, Cyril Bontemps, Arnaud Besserer, Laurence Hotel, Philippe Gérardin, Pierre Leblond
Fungal biodegradation of wood is one of the main threats regarding its use as a material. So far, the detection of this decaying process is empirically assessed by loss of mass, when the fungal attack is advanced and woody structure already damaged. Being able to detect fungal attack on wood in earlier steps is thus of special interest for the wood economy. In this aim, we designed here a new diagnostic tool for wood degradation detection based on the bacterial whole-cell biosensor technology. It was designed in diverting the soil bacteria Streptomyces CebR sensor system devoted to cellobiose detection, a cellulolytic degradation by-product emitted by lignolytic fungi since the onset of wood decaying process...
October 8, 2016: Journal of Biotechnology
Ramón Román, Joan Miret, Federica Scalia, Antoni Casablancas, Martí Lecina, Jordi J Cairó
Efficient production and secretion of recombinant proteins in mammalian cell lines relies in a combination of genetic, metabolic and culture strategy factors. The present work assesses the influence of two key genetic components of expression vectors (promoter and signal peptide) on protein production and secretion effciency in HEK293 cells expressing eGFP as a reporter protein. Firstly, the strength of 3 different promoters was evaluated using transient expression methods. Flow cytometry analysis revealed that the highest level of intracellular protein expression was found when eGFP was under the control of CMV promoter, being 3-times higher in comparison to the rest of the promoters tested...
October 7, 2016: Journal of Biotechnology
Sang-Mo Kang, Sajjad Asaf, Sang-Jun Kim, Byung-Wook Yun, In-Jung Lee
Leifsonia xyli SE134 is a potential plant growth-promoting bacterium isolated from a soil in Daegu, Republic of Korea, which produces large amounts of gibberellin (GA) and indole acetic acid (IAA). In this study, we sequenced the complete genome of L. xyli SE134 by the Pacific Biosciences RSII (PacBio) Single Molecule Real Time (SMRT) sequencing technology. The genome of L. xyli SE134 contains a single chromosome that is 3,596,761bp in length, with 70.2% G+C content. The genome contains 3466 protein-coding genes (CDSs) and 51 rRNA- and 46 tRNA-coding genes...
October 7, 2016: Journal of Biotechnology
Arthit Thani, Yen-Han Lin, Pattana Laopaiboon, Lakkana Laopaiboon
Fermentation redox potential was monitored during cell-recycling continuous ethanol operation. The cell-recycling system (CRS) was operated using two hollow fibre (HF) membranes (pore sizes 0.20 and 0.65μm) at three dilution rates (0.02, 0.04 and 0.08h(-1)). Saccharomyces cerevisiae NP 01 were recycled in the fermenter at a recycle ratio of 0.625. Aeration was provided at 2.5vvm for the first 4h and then further supplied continuously at 0.25vvm. As steady state was established, results showed that the fermentation redox potential was lower for processes employing CRS than those without...
October 6, 2016: Journal of Biotechnology
Aysu Tolun, Zeynep Altintas, Nevzat Artik
Phenolic compounds obtained from fruits have recently gained a great attention due to their bioactive roles. However, they are sensitive and they can be easily affected by physicochemical factors that create a great challenge to incorporate them into the food products. Hence, this work aimed to investigate microencapsulation of these compounds to provide a solution for this problem by improving their stability and protecting them against oxidation, light, moisture and temperature. A lab scale spray-dryer was chosen to produce microcapsules of polyphenols using different dextrose equivalents of maltodextrin and gum arabic as a coating material...
October 5, 2016: Journal of Biotechnology
Kodai Machida, Tomoaki Shigeta, Ayano Kobayashi, Ai Masumoto, Yuna Hidaka, Hiroaki Imataka
Protein misfolding and aggregation is one of the major causes of neurodegenerative disorders such as Alzheimer's disease, Parkinson's disease and Huntington's disease. So far protein aggregation related to these diseases has been studied using animals, cultured cells or purified proteins. In this study, we show that a newly synthesized polyglutamine protein implicated in Huntington's disease forms large aggregates in HeLa cells, and successfully recapitulate the process of this aggregation using a translation-based system derived from HeLa cell extracts...
October 1, 2016: Journal of Biotechnology
Jie-Yu Zhao, Shuang Geng, Lian Xu, Bing Hu, Ji-Quan Sun, Yong Nie, Yue-Qin Tang, Xiao-Lei Wu
Defluviimonas alba cai42(T), isolated from the oil-production water in Xinjiang Oilfield in China, has a strong ability to produce exopolysaccharides (EPS). We hereby present its complete genome sequence information which consists of a circular chromosome and three plasmids. The strain characteristically contains various genes encoding for enzymes involved in EPS biosynthesis, modification, and export. According to the genomic and physiochemical data, it is predicted that the strain has the potential to be utilized in industrial production of microbial EPS...
September 28, 2016: Journal of Biotechnology
Yueying Wang, Jingjing Fu, Qian Zhu, Lei Zhu, Jinshui Zheng, Hualin Liu, Donghai Peng, Lifang Ruan, Ming Sun
Bacillus thuringiensis serovar alesti was used to control caterpillars from 1970s. Here we reported the complete genome of BGSC 4C1, the type strain of this serovar. It has a circular chromosome and six plasmids. The largest plasmid pBMB267 contains five insecticidal crystal protein genes (two copies of cry1Ae, cry1Gb, cry2Ab, and a novel cry1M-type gene) and three vegetative insecticidal protein genes (a novel binary toxin gene operon vip1-vip2 and vip3Aa). Besides, the strain also has many genes encodeing virulence factors, and some secondary metabolite biosynthetic gene clusters involved in synthesis of antimicrobial peptides and bacteriocins...
September 28, 2016: Journal of Biotechnology
Katsunori Namakoshi, Tubasa Nakajima, Katsunori Yoshikawa, Yoshihiro Toya, Hiroshi Shimizu
Synechocystis sp. PCC 6803 is an attractive host for bio-ethanol production. In the present study, a nitrogen starvation approach was applied on an ethanol producing strain for inhibiting the growth, since ethanol production competes with the cell growth. The effect of gene deletions in the glycogen and polyhydroxybutyrate (PHB) synthesis pathways was investigated. Measurements of intracellular glycogen and PHB revealed that the glycogen was accumulated under the nitrogen starvation condition and the gene deletion of glycogen synthesis pathway caused the accumulation of PHB...
September 28, 2016: Journal of Biotechnology
Ziqiang Zheng, Jinshui Zheng, Hualin Liu, Donghai Peng, Ming Sun
Root-knot nematodes (RKNs) which can infect almost all crops lead to huge economic losses in agriculture around the world. Unavailability of effective and environmentally friendly control of RKNs provides an opportunity to nematicidal bacteria. Fictibacillus phosphorivorans G25-29 is a gram-positive and spore-forming bacterium with nematicidal capability against root-knot nematodes and free-living nematode Caenorhabditis elegans. Here, we report the complete genome of F. phosphorivorans G25-29, containing a circular chromosome and encoding nine potential nematicidal factors which may contribute to its nematicidal activity...
September 24, 2016: Journal of Biotechnology
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