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Journal of Biotechnology

Ana Inés Catalán, Ana Karen Malan, Fernando Ferreira, Paul R Gill, Silvia Batista
Polyhydroxyalkanoates (PHAs) are thermoplastic polyesters produced by a wide range of bacteria as carbon and energy reserves. PHA accumulation is typically increased under unbalanced growth conditions and with carbon source in excess. Although polyhydroxybutyrate (PHB) could be used for specific applications, it is brittle and not a useful alternative for plastics like polypropylene. Far more useful polypropylene-like PHAs, are copolymers composed of 3-hydroxybutyrate and 3-hydroxyvalerate, P(3HB-co-3 HV)...
September 18, 2018: Journal of Biotechnology
Hamid Sedighian, Raheleh Halabian, Jafar Amani, Mohammad Heiat, Mohsen Amin, Abbas Ali Imani Fooladi
BACKGROUND AND OBJECTIVE: Aptamers or chemical antibodies are oligonucleotides (DNA or RNA) that are able to bind to various targets with high specificity and affinity such as toxins which are isolated by an in vitro method known as SELEX. To date, there are many SELEX procedures for the isolation of novel aptamers against proteins. However not all modified SELEX are suitable for similar protein based on sequence homology such as staphylococcal enterotoxins. Staphylococcal enterotoxin type A (SEA) is the most prevalent toxin involved in staphylococcal food poisoning (SFP) worldwide...
September 17, 2018: Journal of Biotechnology
Xiwei Tian, Yonghong Wang, Ju Chu, Ali Mohsin, Yingping Zhuang
High titer, productivity and yield are the main pre-requisites of an efficient lactic acid production process. However, the hyperosmotic stress inhibits cell metabolism in the later phase of fermentation. In this study, an osmotic-tolerant mutant named Lactobacillus paracasei NCBIO01-M2 was obtained through a high-throughput screening technology, which exhibited a higher tolerance to osmotic stress due to its more flexible regulation of the unsaturated fatty acid proportion along with the intracellular compatible solute pools...
September 12, 2018: Journal of Biotechnology
Jasmin J Samaras, Bernardo Abecasis, Margarida Serra, Andrea Ducci, Martina Micheletti
Cardiomyocytes (CMs), derived from pluripotent stem cells (PSCs), have the potential to be used in cardiac repair. Addition of physical cues, such as electrical and mechanical stimulations, have proven to significantly effect morphology, density, cardiogenesis, maturity and functionality of differentiated CMs. This work combines rigorous fluid dynamics investigation and flow frequency analysis with iPSC differentiation experiments to identify and quantify the flow characteristics leading to a significant increase of differentiation yield...
September 10, 2018: Journal of Biotechnology
J S Silva, J S Mendes, J A C Correia, M V P Rocha, L Micoli
Cashew apple bagasse (CAB) has been studied as feedstock for the biohydrogen production using Clostridium roseum and the dark fermentation process. Pretreatment with alkaline hydrogen peroxide (CAB-AHP) on raw material and the acid and enzymatic hydrolysis have been taken into account to evaluate the H2 yields. Results show that the acid hydrolysate obtained from CAB produced higher H2 molar yield (HMY) (15 mmolH2 /Lhydrolysate ) than the acid hydrolysate from CAB-AHP (4.99 mmolH2 /Lhydrolysate ), These HMY were noticeably higher than values obtained from the enzymatic hydrolysate of CAB-AHP (1...
September 8, 2018: Journal of Biotechnology
Carlos Gaspar, Jonás I Silva-Marrero, Anna Fàbregas, Montserrat Miñarro, Josep R Ticó, Isabel V Baanante, Isidoro Metón
Glutamate dehydrogenase (GDH) plays a major role in amino acid catabolism. To increase the current knowledge of GDH function, we analysed the effect of GDH silencing on liver intermediary metabolism from gilthead sea bream (Sparus aurata). Sequencing of GDH cDNA from S. aurata revealed high homology with its vertebrate orthologues and allowed us to design short hairpin RNAs (shRNAs) to knockdown GDH expression. Following validation of shRNA-dependent downregulation of S. aurata GDH in vitro, chitosan-tripolyphosphate (TPP) nanoparticles complexed with a plasmid encoding a selected shRNA (pCpG-sh2GDH) were produced to address the effect of GDH silencing on S...
September 6, 2018: Journal of Biotechnology
Sarah C Bernier, Louis-Philippe Morency, Rafael Najmanovich, Christian Salesse
A large number of proteins are expressed in fusion with a tag to perform their purification. Glutathione-S-Transferase (GST) is a widely used tag to achieve passenger protein purification. Accordingly, commonly used commercial expression vectors contain the coding sequence of GST in order to express fusion proteins. However, fusion proteins are sometimes expressed in a truncated form that may result from an incomplete synthesis, proteolytic cleavage or the presence of a functional alternative translation initiation site...
September 6, 2018: Journal of Biotechnology
Yanqun Dong, Qiuying Sun, Yinliang Zhang, Xiaojie Wang, Pu Liu, Yazhong Xiao, Zemin Fang
Gongronella sp. w5 (w5) is a soil fungus isolated from Anhui, China. Here we report the high-quality genome sequence of w5 and its phenotypic characteristics based on genomic information. The genome of w5 consists of 34,723,828 bp assembled into 149 scaffolds and 11,302 predicted protein-coding genes. Genome analysis suggested that w5 may possess host cell infection capacity and maybe a biotrophic fungus that relies on plant sucrose as carbon source. W5 shows the ability of rapid invasion into the plant root cells based on CAZymes analysis...
September 5, 2018: Journal of Biotechnology
Miguel-Angel Guerra-Lupián, Roberto Ruiz-Medrano, José-Abrahán Ramírez-Pool, Francisco-Arturo Ramírez-Ortega, José-Abel López-Buenfil, Emiliano Loeza-Kuk, Oscar Morales-Galván, Claudio Chavarin-Palacio, Jesús Hinojosa-Moya, Beatriz Xoconostle-Cázares
Citrus huanglongbing (HLB) is a devastating disease associated with Candidatus Liberibacter asiaticus spp. (CLas), a bacterium restricted to the sieve tube system of the phloem that is transmitted by the psyllid vector, Diaphorina citri. In this study, the human antimicrobial peptides, lysozyme and β-defensin 2, were targeted to the vascular tissue of Mexican lime (Citrus x aurantifolia [Christm.] Swingle) by fusion to a phloem-restricted protein. Localized expression was achieved, via Agrobacterium tumefaciens-mediated transformation of the stem, which led to protein expression and mobilization within the vascular tissue of heterotrophic tissues...
September 5, 2018: Journal of Biotechnology
Qing-Shan Meng, Chen-Guang Liu, Xin-Qing Zhao, Feng-Wu Bai
Cellulose hydrolysis is a synergetic process performed sequentially by different cellulolytic enzymes including endoglucanases, exoglucanases and glucosidases. Trichoderma reesei has been acknowledged as the best cellulase producer, but cellulase production by T. reesei through submerged fermentation is costly due to intensive energy consumption associated with the process for mixing and aeration, since non-Newtonian fluid properties are developed with mycelial growth. Therefore, engineering the ratio of cellulolytic enzymes in the cocktail for more efficient cellulose hydrolysis is an alternative strategy for reducing cellulase dosage and thus saving cost in enzyme consumption for cellulose hydrolysis...
September 5, 2018: Journal of Biotechnology
Stanislav Miertuš, Miroslav Ondrejovič, Kevan M A Gartland
No abstract text is available yet for this article.
August 31, 2018: Journal of Biotechnology
Anargyros N Moulas, Maria Vaiou
Vitamin D is essential for bone health and has significant roles in non-skeletal health and organ function. Dermal synthesis through exposure to ultraviolet B light is the major natural source of vitamin D, while only a small portion of the necessary amount can be acquired by a diet without fortified foods. In recent years, vitamin D deficiency as a result of lifestyles with inadequate sun exposure, has received increased attention due to its association with the increased risk of serious chronic diseases. This review summarizes our current understanding of food fortification strategies with vitamin D and the resulting health impact...
August 31, 2018: Journal of Biotechnology
Djordje Fira, Ivica Dimkić, Tanja Berić, Jelena Lozo, Slaviša Stanković
Bacteria from the Bacillus group are microorganisms that inhabit a large number of different habitats. They are well known as producers of a wide array of antagonistic compounds of different structures, having between 5 to 8% of the total genome devoted to biosynthesis of secondary metabolites. Most important bioactive molecules from the genus Bacillus are non-ribosomally synthesized peptides and lipopeptides, polyketide compounds, bacteriocins and siderophores. Lipopeptides from Bacillus have very complex mechanisms of biosynthesis catalyzed by non-ribosomal peptide synthetases (NRPSs), large enzyme complexes with modular structure, with each module being in charge for the incorporation of a particular amino acid...
August 30, 2018: Journal of Biotechnology
Christel Aebischer-Gumy, Pierre Moretti, Thomas A Little, Martin Bertschinger
Cell populations that are exclusively composed by descendants from a defined single parental progenitor are referred to as "monoclonal", "clonal" or more correctly as "clonally derived cell population". Clonal derivation of cell lines used in the manufacturing of recombinant biologics is a regulatory requirement that aims to ensure a robust process and consistent quality throughout the life cycle of a product. Clonal derivation of cell lines is usually ensured by the process (e...
August 30, 2018: Journal of Biotechnology
Qi Zhao, Yiwei Meng, Shannan Li, Peiwen Lv, Ping Xu, Chunyu Yang
Halophilic microorganisms have great potentials towards biotechnological applications. Halomonas hydrothermalis Y2 is a halotolerant and alkaliphilic strain that isolated from the Na+ -rich pulp mill wastewater. The strain is dominant in the bacterial community of pulp mill wastewater and exhibits metabolic diversity in utilizing various substrates. Here we present the genome sequence of this strain, which comprises a circular chromosome 3,933,432 bp in size and a GC content of 60.2%. Diverse genes that encoding proteins for compatible solutes synthesis and transport were identified from the genome...
August 30, 2018: Journal of Biotechnology
Barbora Legerská, Daniela Chmelová, Miroslav Ondrejovič
The decolourization and detoxification of azo dyes (Orange 2, Acid Orange 6) by fungal laccase from Trametes versicolor were evaluated. For laccase catalysed reaction, the azonaphthol Orange 2, with 72.8% decolourization, was degraded more rapidly than the azobenzene Acid Orange 6, with 45.3%. The presence of hydroxyl group at o-position to azo bond in the structure of Orange 2 was more preferable than the presence of two hydroxyl groups at o- and p-positions to azo bond in Acid Orange 6. Although the laccase treatment was more effective for the Orange 2 decolourization, the toxicity of both monoazo dye solutions became less toxic for the prokaryote growth...
August 29, 2018: Journal of Biotechnology
Marcos José Chaprão, Rita de Cássia Freire Soares da Silva, Raquel Diniz Rufino, Juliana Moura Luna, Valdemir Alexandre Santos, Leonie Asfora Sarubbo
The present study describes the formulation of a biosurfactant produced by Bacillus methylotrophicus UCP1616 and investigates its long-term stability for application as a collector in a bench-scale dissolved air flotation (DAF) prototype. For formulation, the conservative potassium sorbate was added to the biosurfactant with or without prior heat treatment at 80 °C for 30 min. After formulation, the biosurfactant samples were stored at room temperature for 180 days and the tensioactive properties of the biomolecule were determined with different pH values, temperatures and concentrations of salt...
August 29, 2018: Journal of Biotechnology
Wenzhe Zhang, Benyi Xiao, YanBo Li, Yu Liu, Xuesong Guo
Three alkaline treatments (pH 10, 11, and 12 for 1 h) were used to treat return sludge alone to reduce sludge production in laboratory-scale anaerobic-anoxic-oxic processes. After 99 days of operation, alkaline treatments at pH 10 and 11 led to accumulative excess sludge production and sludge yield reduction of 18.8%-31.7% and 14.7%-27.8%, respectively. However, alkaline treatment at pH 12 led to system breakdown because of sludge bulking. The alkaline treatment at pH 10 did not affect the chemical oxygen demand and NH4 + -N removals of the system and sludge volume index (SVI) of aerobic activated sludge...
August 28, 2018: Journal of Biotechnology
Daniel Baur, James M Angelo, Srinivas Chollangi, Xuankuo Xu, Thomas Müller-Späth, Na Zhang, Sanchayita Ghose, Zheng Jian Li, Massimo Morbidelli
Effect of particle size (85μm vs. 50μm) on the performance of continuous capture chromatography using Protein A affinity was evaluated in combination with varied feed titers, loading flow rates and target breakthrough using a Design of Experiments (DoE) approach. In comparison to previous studies, higher cell culture titers on the order of 5-15 g/L, relevant to current high productivity industrial cell lines, were evaluated. Further, three modes of capture continuous chromatography were included in the DoE: single column batch, 2-column CaptureSMB and 4-column periodic counter-current chromatography (PCC)...
August 27, 2018: Journal of Biotechnology
Marta M Silva, Patrícia Gomes-Alves, Sara Rosa, Daniel Simão, José M Inácio, Cristina Peixoto, Margarida Serra, José A Belo, Paula M Alves
Collagen and calcium-binding EGF domain-1 (CCBE1) is a secreted protein critical for lymphatic/cardiac vascular development and regeneration. However, the low efficient production of the recombinant full-length CCBE1 (rCCBE1) has been a setback for functional studies and therapeutic applications using this protein. The main goal of this work was to implement a robust bioprocess for efficient production of glycosylated rCCBE1. Different bioprocess strategies were combined with proteomic tools for process/product characterization, evaluating the impact of process parameters on cell performance, rCCBE1 production and quality...
August 27, 2018: Journal of Biotechnology
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