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European Biophysics Journal: EBJ

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https://www.readbyqxmd.com/read/28929205/simulation-of-the-osmosis-based-drug-encapsulation-in-erythrocytes
#1
Duobiao Ge, Lili Zou, Chengpan Li, Sen Liu, Shibo Li, Sijie Sun, Weiping Ding
Drug-loaded erythrocytes have been proposed for the treatment of disease. A common way to load drugs into erythrocytes is to apply osmotic shock. Currently, osmosis-based drug encapsulation is studied mainly experimentally, whereas a related theoretical model is still incomplete. In this study, a set of equations is developed to simulate the osmosis-based drug-encapsulation process. First, the modeling is validated with hemolysis rates and the drug-loaded quantities to be found in the literature. Then, the variation of the erythrocyte volume, formation of the pore on the erythrocyte membrane, and quantities of drug loaded into and hemoglobin released from erythrocytes are studied...
September 20, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/28916873/effects-of-methotrexate-on-the-quality-of-oocyte-maturation-in-vitro
#2
N Tian, J Yu, Sh Zhang, W Y Ma, T Wang, Y M Wang
Methotrexate (MTX), an antifolate drug, is widely used for clinical treatment of malignancies and ectopic pregnancy. Many studies have documented that MTX has strong side-effects on rapidly dividing somatic cells. However, its side-effects on female reproductive cells have not been widely reported. Combined with in vitro culture, two-photon fluorescence imaging and three-dimensional reconstruction, this study analyzed the effects of MTX on oocyte maturation time, chromosome arrangement, karyotype, spindle morphology, and the localization of microtubule organizing centers (MTOCs)...
September 15, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/28913625/calcium-release-dependent-inactivation-precedes-formation-of-the-tubular-system-in-developing-rat-cardiac-myocytes
#3
Katarina Macková, Alexandra Zahradníková, Matej Hoťka, Barbora Hoffmannová, Ivan Zahradník, Alexandra Zahradníková
Developing cardiac myocytes undergo substantial structural and functional changes transforming the mechanism of excitation-contraction coupling from the embryonic form, based on calcium influx through sarcolemmal DHPR calcium channels, to the adult form, relying on local calcium release through RYR calcium channels of sarcoplasmic reticulum stimulated by calcium influx. We characterized day-by-day the postnatal development of the structure of sarcolemma, using techniques of confocal fluorescence microscopy, and the development of the calcium current, measured by the whole-cell patch-clamp in isolated rat ventricular myocytes...
September 14, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/28905203/adaptive-resolution-simulations-of-biomolecular-systems
#4
Julija Zavadlav, Staš Bevc, Matej Praprotnik
In this review article, we discuss and analyze some recently developed hybrid atomistic-mesoscopic solvent models for multiscale biomolecular simulations. We focus on the biomolecular applications of the adaptive resolution scheme (AdResS), which allows solvent molecules to change their resolution back and forth between atomistic and coarse-grained representations according to their positions in the system. First, we discuss coupling of atomistic and coarse-grained models of salt solution using a 1-to-1 molecular mapping-i...
September 13, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/28889232/modulation-of-the-flavin-protein-interactions-in-nadh-peroxidase-and-mercuric-ion-reductase-a-resonance-raman-study
#5
Julie Keirsse-Haquin, Thierry Picaud, Luc Bordes, Adrienne Gomez de Gracia, Alain Desbois
NADH peroxidase (Npx) and mercuric ion reductase (MerA) are flavoproteins belonging to the pyridine nucleotide:disulfide oxidoreductases (PNDO) and catalyzing the reduction of toxic substrates, i.e., hydrogen peroxide and mercuric ion, respectively. To determine the role of the flavin adenine dinucleotide (FAD) in the detoxification mechanism, the resonance Raman (RR) spectra of these enzymes under various redox and ligation states have been investigated using blue and/or near-UV excitation(s). These data were compared to those previously obtained for glutathione reductase (GR), another enzyme of the PNDO family, but catalyzing the reduction of oxidized glutathione...
September 9, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/28889176/proton-pump-inhibitors-have-ph-dependent-effects-on-the-thermostability-of-the-carboxyl-terminal-domain-of-voltage-gated-proton-channel-hv1
#6
Qing Zhao, Weiyan Zuo, Shangrong Zhang, Yongqiang Zhang, Chuanyong Li, Shu Jie Li
The voltage-gated proton channel Hv1 is highly selective for H(+) and is activated by membrane depolarization and pH gradient. An increased external and decreased internal pH opens the Hv1 channel. The intracellular C-terminal domain of Hv1 is responsible for channel dimerization, cooperative, and thermosensitive gating. Here, we found that proton pump inhibitors (PPIs) interact with the C-terminal domain of human Hv1. The interaction between PPIs and the C-terminal domain, which is pH-dependent, lowered the thermal and structural stability of the protein at pH 4, but enhanced the thermal and structural stability at pH 8...
September 9, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/28875401/mechanism-of-salt-induced-activity-enhancement-of-a-marine-derived-laccase-lac15
#7
Jie Li, Yanan Xie, Rui Wang, Zemin Fang, Wei Fang, Xuecheng Zhang, Yazhong Xiao
Laccase (benzenediol: oxygen oxidoreductases, EC1.10.3.2) is a multi-copper oxidase capable of oxidizing a variety of phenolic and other aromatic organic compounds. The catalytic power of laccase makes it an attractive candidate for potential applications in many areas of industry including biodegradation of organic pollutants and synthesis of novel drugs. Most laccases are vulnerable to high salt and have limited applications. However, some laccases are not only tolerant to but also activated by certain concentrations of salt and thus have great application potential...
September 5, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/28866771/atomic-force-microscopy-analysis-of-extracellular-vesicles
#8
P Parisse, I Rago, L Ulloa Severino, F Perissinotto, E Ambrosetti, P Paoletti, M Ricci, A P Beltrami, D Cesselli, L Casalis
Extracellular vesicles (EVs) are small vesicles ensuring transport of molecules between cells and throughout the body. EVs contain cell type-specific signatures and have been proposed as biomarkers in a variety of diseases. Their small size (<1 μm) and biological and physical functions make them obvious candidates for therapeutic agents in immune therapy, vaccination, regenerative medicine and drug delivery. However, due to the complexity and heterogeneity of their origin and composition, the actual mechanism through which these vesicles exert their functions is still unknown and represents a great biomedical challenge...
September 2, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/28865004/gold-nanoparticles-with-patterned-surface-monolayers-for-nanomedicine-current-perspectives
#9
REVIEW
Paolo Pengo, Maria Şologan, Lucia Pasquato, Filomena Guida, Sabrina Pacor, Alessandro Tossi, Francesco Stellacci, Domenico Marson, Silvia Boccardo, Sabrina Pricl, Paola Posocco
Molecular self-assembly is a topic attracting intense scientific interest. Various strategies have been developed for construction of molecular aggregates with rationally designed properties, geometries, and dimensions that promise to provide solutions to both theoretical and practical problems in areas such as drug delivery, medical diagnostics, and biosensors, to name but a few. In this respect, gold nanoparticles covered with self-assembled monolayers presenting nanoscale surface patterns-typically patched, striped or Janus-like domains-represent an emerging field...
September 1, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/28825121/mutagenesis-of-the-nachbac-sodium-channel-discloses-a-functional-role-for-a-conserved-s6-asparagine
#10
Andrias O O'Reilly, Anja Lattrell, Andrew J Miles, Alexandra B Klinger, Carla Nau, B A Wallace, Angelika Lampert
Asparagine is conserved in the S6 transmembrane segments of all voltage-gated sodium, calcium, and TRP channels identified to date. A broad spectrum of channelopathies including cardiac arrhythmias, epilepsy, muscle diseases, and pain disorders is associated with its mutation. To investigate its effects on sodium channel functional properties, we mutated the simple prokaryotic sodium channel NaChBac. Electrophysiological characterization of the N225D mutant reveals that this conservative substitution shifts the voltage-dependence of inactivation by 25 mV to more hyperpolarized potentials...
August 20, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/28819704/shining-light-on-membrane-proteins
#11
(no author information available yet)
No abstract text is available yet for this article.
August 17, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/28752207/conformational-changes-from-%C3%AE-strand-to-%C3%AE-helix-of-the-fatty-acid-binding-protein-rep1-ncxsq-in-anionic-lipid-membranes-dependence-with-the-vesicle-curvature
#12
Vanesa V Galassi, Silvina R Salinas, Guillermo G Montich
We studied the conformational changes of the fatty acid-binding protein ReP1-NCXSQ in the interface of anionic lipid membranes. ReP1-NCXSQ is an acidic protein that regulates the activity of the Na(+)/Ca(2+) exchanger in squid axon. The structure is a flattened barrel composed of two orthogonal β-sheets delimiting an inner cavity and a domain of two α-helix segments arranged as a hairpin. FTIR and CD spectroscopy showed that the interactions with several anionic lipids in the form of small unilamellar vesicles (SUVs) induced an increase in the proportion of helix secondary structure...
July 27, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/28725998/membrane-phase-transition-during-heating-and-cooling-molecular-insight-into-reversible-melting
#13
Liping Sun, Rainer A Böckmann
With increasing temperature, lipid bilayers undergo a gel-fluid phase transition, which plays an essential role in many physiological phenomena. In the present work, this first-order phase transition was investigated for variable heating and cooling rates for a dipalmitoylphosphatidylcholine (DPPC) lipid bilayer by means of atomistic molecular dynamics simulations. Alternative methods to track the melting temperature [Formula: see text] are compared. The resulting [Formula: see text] is shown to be independent of the scan rate for small heating rates (0...
July 19, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/28725997/mitotic-spindle-kinetochore-fibers-hold-on-tight-to-interpolar-bundles
#14
REVIEW
Iva M Tolić
When a cell starts to divide, it forms a spindle, a micro-machine made of microtubules, which separates the duplicated chromosomes. The attachment of microtubules to chromosomes is mediated by kinetochores, protein complexes on the chromosome. Spindle microtubules can be divided into three major classes: kinetochore microtubules, which form k-fibers ending at the kinetochore; interpolar microtubules, which extend from the opposite sides of the spindle and interact in the middle; and astral microtubules, which extend towards the cell cortex...
July 19, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/28712066/effect-of-ph-and-ionic-strength-on-the-binding-strength-of-anti-pf4-polyanion-antibodies
#15
Thi-Huong Nguyen, Andreas Greinacher
Antibodies against the chemokine platelet factor 4 (PF4) in complex with heparin cause the prothrombotic adverse drug reaction heparin-induced thrombocytopenia (HIT). The current dilemma for laboratory diagnosis of HIT is that only about 50% of these antibodies are pathogenic, but the widely available enzyme immunosorbent assays (EIA), used for diagnosis in the clinical laboratory cannot differentiate between non-pathogenic- and pathogenic (platelet activating) antibodies. It would be highly desirable to improve diagnosis for HIT...
July 15, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/28712065/erratum-to-stochastic-phenotypic-interconversion-in-tumors-can-generate-heterogeneity
#16
Giuseppina Simone
No abstract text is available yet for this article.
July 15, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/28710521/efflux-drug-transporters-at-the-forefront-of-antimicrobial-resistance
#17
REVIEW
Tahmina Rahman, Benjamin Yarnall, Declan A Doyle
Bacterial antibiotic resistance is rapidly becoming a major world health consideration. To combat antibiotics, microorganisms employ their pre-existing defence mechanisms that existed long before man's discovery of antibiotics. Bacteria utilise levels of protection that range from gene upregulation, mutations, adaptive resistance, and production of resistant phenotypes (persisters) to communal behaviour, as in swarming and the ultimate defence of a biofilm. A major part of all of these responses involves the use of antibiotic efflux transporters...
July 14, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/28698919/unperturbed-hydrocarbon-chains-and-liquid-phase-bilayer-lipid-chains-a-computer-simulation-study
#18
Alexander L Rabinovich, Alexander P Lyubartsev, Dmitrii V Zhurkin
In this work, the properties of saturated and unsaturated fatty acid acyl chains 16:0, 18:0, 18:1(n-9)cis, 18:2(n-6)cis, 18:3(n-3)cis, 18:4(n-3)cis, 18:5(n-3)cis, 20:4(n-6)cis, 20:5(n-3)cis and 22:6(n-3)cis in a bilayer liquid crystalline state and similar hydrocarbon chains (with CH[Formula: see text] terminal groups instead of C=O groups) in the unperturbed state characterised by a lack of long-range interaction were investigated. The unperturbed hydrocarbon chains were modelled by Monte Carlo simulations at temperature [Formula: see text] K; sixteen fully hydrated homogeneous liquid crystalline phosphatidylcholine bilayers containing these chains were studied by molecular dynamics simulations at the same temperature...
July 11, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/28695249/first-study-on-iron-complexes-in-blood-and-organ-samples-from-thalassaemic-and-normal-laboratory-mice-using-m%C3%A3-ssbauer-spectroscopy
#19
George Charitou, Vlassis Petousis, Charalambos Tsertos, Yannis Parpottas, Marina Kleanthous, Marios Phylactides, Soteroula Christou
Measurements of iron complexes and iron stores in the body are crucial for evaluation and management of chelation therapy targeted against iron accumulation or overload in blood and organs. In this work, blood and tissue samples from one normal and one thalassaemic laboratory mouse were studied using (57)Fe Mössbauer spectroscopy at 78 K for the first time. In contrast to human patients, these laboratory mice did not receive any medical treatment, thus the iron components present in the samples are not altered from their natural state...
July 10, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/28695248/divergent-effects-of-anesthetics-on-lipid-bilayer-properties-and-sodium-channel-function
#20
REVIEW
Karl F Herold, Olaf S Andersen, Hugh C Hemmings
General anesthetics revolutionized medicine by allowing surgeons to perform more complex and much longer procedures. This widely used class of drugs is essential to patient care, yet their exact molecular mechanism(s) are incompletely understood. One early hypothesis over a century ago proposed that nonspecific interactions of anesthetics with the lipid bilayer lead to changes in neuronal function via effects on membrane properties. This model was supported by the Meyer-Overton correlation between anesthetic potency and lipid solubility and despite more recent evidence for specific protein targets, in particular ion-channels, lipid bilayer-mediated effects of anesthetics is still under debate...
July 10, 2017: European Biophysics Journal: EBJ
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