Read by QxMD icon Read

Applied Microbiology and Biotechnology

Yujie Zhou, Hong Yang, Xuedong Zhou, Hongke Luo, Fan Tang, Jin Yang, Gil Alterovitz, Lei Cheng, Biao Ren
The increase of fungal infectious diseases and lack of safe and efficacious antifungal drugs result in the urgent need of new therapeutic strategies. Here, we repurposed the lovastatin (LOV) as a synergistic antifungal potentiator to itraconazole (ITZ) against Candida albicans planktonic cells and biofilms in vitro for the first time. Mutants from ergosterol biosynthesis pathway were employed and key gene expression profiles of ergosterol pathway were also measured. LOV single treatment was unable to inhibit C...
April 25, 2018: Applied Microbiology and Biotechnology
Dongdong Mu, Jiaojiao Lu, Mingqiang Qiao, Oscar P Kuipers, Jing Zhu, Xingjiang Li, Peizhou Yang, Yanyan Zhao, Shuizhong Luo, Xuefeng Wu, Shaotong Jiang, Zhi Zheng
Microbial transglutaminase (MTG) from Streptomyces mobaraensis has been widely used for crosslinking proteins in order to acquire products with improved properties. To improve the yield and enable a facile and efficient purification process, recombinant vectors, harboring various heterologous signal peptide-encoding fragments fused to the mtg gene, were constructed in Escherichia coli and then expressed in Bacillus subtilis. Signal peptides of both WapA and AmyQ (SP wapA and SP amyQ ) were able to direct the secretion of pre-pro-MTG into the medium...
April 25, 2018: Applied Microbiology and Biotechnology
Reeta Davis, Susan Molloy, Blathnaid Quigley, Jasmina Nikodinovic-Runic, Francisco Solano, Kevin E O'Connor
We evaluated the kinetic characteristics of wild type (WT) and three engineered variants (RVC10, RV145, and C10_N322S) of tyrosinase from Ralstonia solanacearum and their potential as biocatalysts to produce halogenated catechols. RV145 exhibited a 3.6- to 14.5-fold improvement in catalytic efficiency (kcat /Km ) with both reductions in Km and increases in kcat compared to WT, making it the best R. solanacearum tyrosinase variant towards halogenated phenols. RVC10 also exhibited increases in catalytic efficiency with all the tested phenols...
April 24, 2018: Applied Microbiology and Biotechnology
Ruixin Mao, Shuangsheng Guo
The effect of mixed light quality with red, blue, and green LED lamps on the growth of Arthrospira platensis was studied, so as to lay the theoretical and technical basis for establishing a photo-bioreactor lighting system for application in space. Meanwhile, indexes, like morphology, growth rate, photosynthetic pigment compositions, energy efficiency, and main nutritional components, were measured respectively. The results showed that the blue light combined with red light could decrease the tightness of filament, and the effect of green light was opposite...
April 24, 2018: Applied Microbiology and Biotechnology
Luisana Avilan, Baptiste Roumezi, Véronique Risoul, Christophe Sébastien Bernard, Arlette Kpebe, Mayssène Belhadjhassine, Marc Rousset, Myriam Brugna, Amel Latifi
The conversion of solar energy into hydrogen represents a highly attractive strategy for the production of renewable energies. Photosynthetic microorganisms have the ability to produce H2 from sunlight but several obstacles must be overcome before obtaining a sustainable and efficient H2 production system. Cyanobacteria harbor [NiFe] hydrogenases required for the consumption of H2 . As a result, their H2 production rates are low, which makes them not suitable for a high yield production. On the other hand, [FeFe] enzymes originating from anaerobic organisms such as Clostridium exhibit much higher H2 production activities, but their sensitivity to O2 inhibition impairs their use in photosynthetic organisms...
April 24, 2018: Applied Microbiology and Biotechnology
Ruo-Nan Wu, Han Meng, Yong-Feng Wang, Ji-Dong Gu
Forest ecosystems have great ecological values in mitigation of climate change and protection of biodiversity of flora and fauna; re-forestry is commonly used to enhance the sequestration of atmospheric CO2 into forest storage biomass. Therefore, seasonal and spatial dynamics of the major microbial players in nitrification, ammonia-oxidizing archaea (AOA) and bacteria (AOB), in acidic soils of young and matured revegetated forests were investigated to elucidate the changes of microbial communities during forest restoration, and compared to delineate the patterns of community shifts under the influences of environmental factors...
April 24, 2018: Applied Microbiology and Biotechnology
Agnieszka Szopa, Marta Klimek-Szczykutowicz, Adam Kokotkiewicz, Anna Maślanka, Agata Król, Maria Luczkiewicz, Halina Ekiert
In the presented work, raw materials (fruits and leaves) and in vitro biomass of a highly productive Schisandra chinensis Sadova No. 1 cultivar (SchS) were evaluated for the production of therapeutically useful schisandra lignans (SL). In vitro cultures of SchS were initiated, followed by extensive optimization studies focused on maximizing secondary metabolite production, with the aim of establishing a sustainable source of SL. Different cultivation systems (agar, agitated, bioreactor), experiment times (10, 20, 30, 40, 50 and 60 days) and plant growth regulators (6-benzyladenine-BA and 1-naphthaleneacetic acid-NAA, from 0 to 3 mg/l) in Murashige-Skoog (MS) medium were tested...
April 23, 2018: Applied Microbiology and Biotechnology
Simona Varriale, Gabriella Cerullo, Io Antonopoulou, Paul Christakopoulos, Ulrika Rova, Thierry Tron, Régis Fauré, Peter Jütten, Alexander Piechot, Joana L A Brás, Carlos M G A Fontes, Vincenza Faraco
The chemical syntheses currently employed for industrial purposes, including in the manufacture of cosmetics, present limitations such as unwanted side reactions and the need for harsh chemical reaction conditions. In order to overcome these drawbacks, novel enzymes are developed to catalyze the targeted bioconversions. In the present study, a methodology for the construction and the automated screening of evolved variants library of a Type B feruloyl esterase from Myceliophthora thermophila (MtFae1a) was developed and applied to generation of 30,000 mutants and their screening for selecting the variants with higher activity than the wild-type enzyme...
April 23, 2018: Applied Microbiology and Biotechnology
Louwrens Wiid Theron, Marina Bely, Benoit Divol
The perception of haze in wine is brought about when pathogenesis-related proteins become unstable and aggregate, subsequently resulting in crosslinking until it develops into light-dispersing particles. Elimination of these proteins is usually achieved via bentonite fining, which, although effective, suffers from several drawbacks. The utilization of proteases has been proposed as an ideal alternative. In a previous study, an aspartic protease (MpAPr1) from the yeast Metschnikowia pulcherrima was purified and shown to be partially active against grape proteins in synthetic medium...
April 23, 2018: Applied Microbiology and Biotechnology
Elham Esmaeilishirazifard, Aliakbar Dariush, Sterghios A Moschos, Tajalli Keshavarz
Quorum sensing molecules (QSMs) regulate, through a chemical communication process, multiple complex systems in bacterial and some fungal populations on the basis of cell density. The bacterial QSMs involved in inter-kingdom cross-talk may exhibit antagonistic activity against fungi. This provides an important opportunity for biocontrol of fungal invasion in plants. It has been shown that cultures of Bacillus spp. inhibit fungal growth. Here, we explore the inhibitory potential of the industrial workhorse Bacillus licheniformis NCIMB-8874 and its QSM (ComX pheromone) on the growth of Aspergillus flavus, a cereal, legume, and nut crop pathogen...
April 23, 2018: Applied Microbiology and Biotechnology
Xiaoqing Yang, Zhen Liu, Chengcheng Jiang, Jianan Sun, Changhu Xue, Xiangzhao Mao
β-Galactosidases have a wide application in the food and pharmaceutical industries. Recently, β-galactosidase was also found to participate in agar degradation. In this study, the second reported agarolytic β-galactosidase was found in the marine bacterium Agarivorans gilvus WH0801 and characterized. The β-galactosidase named AgWH2A (83 kDa) exhibited good activities under optimal hydrolysis conditions of pH 8.0 and 40 °C. AgWH2A could cleave the first D-galactose of agarooligosaccharides from its nonreducing end to produce neoagarooligosaccharides, but could not act on the neoagarooligosaccharides...
April 23, 2018: Applied Microbiology and Biotechnology
Johan Morrill, Anna Månberger, Anna Rosengren, Polina Naidjonoka, Pernille von Freiesleben, Kristian B R M Krogh, Karl-Erik Bergquist, Tommy Nylander, Eva Nordberg Karlsson, Patrick Adlercreutz, Henrik Stålbrand
β-Mannanases catalyze the conversion and modification of β-mannans and may, in addition to hydrolysis, also be capable of transglycosylation which can result in enzymatic synthesis of novel glycoconjugates. Using alcohols as glycosyl acceptors (alcoholysis), β-mannanases can potentially be used to synthesize alkyl glycosides, biodegradable surfactants, from renewable β-mannans. In this paper, we investigate the synthesis of alkyl mannooligosides using glycoside hydrolase family 5 β-mannanases from the fungi Trichoderma reesei (TrMan5A and TrMan5A-R171K) and Aspergillus nidulans (AnMan5C)...
April 22, 2018: Applied Microbiology and Biotechnology
Ágnes Duzs, András Tóth, Brigitta Németh, Tímea Balogh, Péter B Kós, Gábor Rákhely
Sulfide detoxification can be catalyzed by ancient membrane-bound flavoproteins, sulfide:quinone oxidoreductases (Sqr), which have important roles in sulfide homeostasis and sulfide-dependent energy conservation processes by transferring electrons from sulfide to respiratory or photosynthetic membrane electron flow. Sqr enzymes have been categorized into six groups. Several members of the groups I, II, III, and V are well-known, but type IV and VI Sqrs are, as yet, uncharacterized or hardly characterized at all...
April 21, 2018: Applied Microbiology and Biotechnology
Valery M Dembitsky, Nick Savidov, Vladimir V Poroikov, Tatyana A Gloriozova, Andrew B Imbs
The present review describes the distribution and biological activities of natural mono-, di-, and triaromatic steroids. It is shown that the producers of aromatic steroids are microorganisms, fungi, and marine invertebrates, and also they were found in plants, animals, marine sediments, and karst deposits. Eighty biologically active aromatic steroids likely have an anti-tumor, anti-inflammatory, and neuroprotection activity with a confidence of 78 to 92%. The structures and predicted biological activities of aromatic steroids are available...
April 21, 2018: Applied Microbiology and Biotechnology
Nadeshda Andreeva, Lubov Ryazanova, Anton Zvonarev, Ludmila Trilisenko, Tatiana Kulakovskaya, Mikhail Eldarov
Inorganic polyphosphate (polyP) is a significant regulatory and metabolic compound in yeast cells. We compared polyP content and localization, polyphosphatase activities, and transcriptional profile of polyP-related genes in industrially important methylotrophic yeasts, Hansenula polymorpha and Pichia pastoris. The increased need for phosphate, the decrease of long-chain polyP level, the accumulation of short-chain polyP, and enhanced endopolyphosphatase activity in the crude membrane fraction were observed in methanol-grown cells compared with glucose-grown cells of both species...
April 21, 2018: Applied Microbiology and Biotechnology
Hongwei Guo, Tianqiu Huang, Jun Zhao, Hongwen Chen, Guo Chen
Short-chain carboxylic acids and their derivatives are widely utilized in all aspects of our daily life. Given their specific functional groups, these molecules are also utilized in fine chemical synthesis. The traditional petroleum-based carboxylate production methods are restricted by petrol shortage and environmental pollution. Renowned for their more sustainable processes than traditional methods, biotechnological methods are preferred alternatives and have attracted increasing attention. However, the industrial application of biotechnological methods is currently limited by low factors: low productivity and low yield...
April 20, 2018: Applied Microbiology and Biotechnology
Kang Zhang, Lingqia Su, Jing Wu
To study the effect of proteases on pullulanase production, six protease-encoding genes (nprB, bpr, mpr, epr, vpr, and wprA) in the genome of Bacillus subtilis strain WS5, which already lacks the protease-encoding genes nprE and aprE, were sequentially disrupted using a CRISPR/Cas9 system. This created strains WS6-WS11, respectively. The strains WS3 (none) and WS4 (ΔnprE) were constructed earlier. After addition of expression plasmid pHYPULd4 into the strains WS3-WS11, the pullulanase production levels of the resulting strains (WS3PUL-WS11PUL, respectively) were investigated in shake-flask cultivations and recombinant strain WS5PUL produced the highest pullulanase activity (148...
April 20, 2018: Applied Microbiology and Biotechnology
Wen-Tao Yang, Gui-Lian Yang, Liang Zhao, Yu-Bei Jin, Yan-Long Jiang, Hai-Bin Huang, Chun-Wei Shi, Jian-Zhong Wang, Guan Wang, Yuan-Huan Kang, Chun-Feng Wang
Avian influenza virus (AIV) can infect poultry, mammals, and other hosts and causes enormous economic losses to the global poultry industry. In this study, to develop a novel and potent oral vaccine based on Lactobacillus plantarum (L. plantarum) for controlling the spread of AIV in the poultry industry, we constructed a recombinant L. plantarum strain displaying the 3M2e-HA2 protein of the influenza virus and determined the effect of N/pgsA'-3M2e-HA2 against AIV in chicks. We first confirmed that the 3M2e-HA2 fusion protein was expressed on the surface of L...
April 19, 2018: Applied Microbiology and Biotechnology
Monize Caiado Decarli, Diogo Peres Dos Santos, Renato Mancini Astray, Daniella Cristina Ventini-Monteiro, Soraia Attie Calil Jorge, Daniela Matilde Correia, Juliana de Sá da Silva, Mayra Pereira Rocca, Hélio Langoni, Benedito Donizete Menozzi, Carlos Augusto Pereira, Claudio Alberto Torres Suazo
The transmembrane rabies virus glycoprotein (RVGP) is the main antigen of vaccine formulations used around the world to prevent rabies, the most lethal preventable infectious disease known. The objective of this work was to evaluate the potential of a bioreactor using wave-induced agitation in the initial steps of scaling up the rRVGP production process by a Drosophila melanogaster S2 cell line to produce rRVGP in sufficient quantities for immunization and characterization studies. Taking advantage of some remarkable features recognized in Drosophila S2 cells for scaling the culture process, a robust recombinant lineage (S2MtRVGPH-His) engineered by our group for the expression of rRVGP using a copper-inducible promoter was used in the bioreactor cultures...
April 19, 2018: Applied Microbiology and Biotechnology
Suan Shi, Jing Li, David M Blersch
The aim of this work was to study the solid waste (manure) produced by catfish as a potential feedstock for the production of lactic acid (LA) via fermentation. The solid waste contains high levels of both carbohydrates and nutrients that are sufficient for LA bacteria. Simultaneous saccharification and co-fermentation (SSCF) was applied using enzyme and Lactobacillus pentosus, and different loadings of enzyme and solid waste were tested. Results showed LA concentrations of 35.7 g/L were obtained at 15% solids content of catfish waste...
April 19, 2018: Applied Microbiology and Biotechnology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"