journal
MENU ▼
Read by QxMD icon Read
search

Applied Microbiology and Biotechnology

journal
https://www.readbyqxmd.com/read/28108764/letr-is-a-tetr-family-transcription-factor-from-lysobacter-controlling-antifungal-antibiotic-biosynthesis
#1
Ping Wang, Hongfu Chen, Guoliang Qian, Fengquan Liu
Heat-stable antifungal factor (HSAF) is a newly identified and broad-spectrum antifungal antibiotic from Lysobacter enzymogenes, a ubiquitous environmental proteobacterium. Yet, the regulatory mechanism for HSAF biosynthesis in L. enzymogenes remains poorly understood. Here, we report the identification of a TetR-family protein Le1552 (LetR) from L. enzymogenes strain OH11 that is involved in transcriptional repression of HSAF production. Bacterial one-hybrid and gel mobility shift assays show that LetR directly binds to PHSAF (the promoter region of the HSAF biosynthesis operon)...
January 20, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28108763/carbon-source-regulation-of-gene-expression-in-methylosinus-trichosporium-ob3b
#2
Muhammad Farhan Ul Haque, Wenyu Gu, Bipin S Baral, Alan A DiSpirito, Jeremy D Semrau
Gene expression in methanotrophs has been shown to be affected by the availability of a variety of metals, most notably copper regulating expression of alternative forms of methane monooxygenase. Here, we show that growth substrate also affects expression of genes encoding for enzymes responsible for the oxidation of methane to formaldehyde and the assimilation of carbon. Specifically, in Methylosinus trichosporium OB3b, expression of genes involved in the conversion of methane to methanol (pmoA and mmoX) and methanol to formaldehyde (mxaF, xoxF1, and xoxF2) as well as in carbon assimilation (fae1, fae2, metF, and sga) decreased when this strain was grown on methanol vs...
January 20, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28108762/enhancement-of-succinate-yield-by-manipulating-nadh-nad-ratio-and-atp-generation
#3
Jiaojiao Li, Yikui Li, Zhiyong Cui, Quanfeng Liang, Qingsheng Qi
We previously engineered Escherichia coli YL104 to efficiently produce succinate from glucose. In this study, we investigated the relationships between the NADH/NAD(+) ratio, ATP level, and overall yield of succinate production by using glucose as the carbon source in YL104. First, the use of sole NADH dehydrogenases increased the overall yield of succinate by 7% and substantially decreased the NADH/NAD(+) ratio. Second, the soluble fumarate reductase from Saccharomyces cerevisiae was overexpressed to manipulate the anaerobic NADH/NAD(+) ratio and ATP level...
January 20, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28105487/biotransformation-of-%C3%AE-and-%C3%AE-pinene-into-flavor-compounds
#4
REVIEW
Kele A C Vespermann, Bruno N Paulino, Mayara C S Barcelos, Marina G Pessôa, Glaucia M Pastore, Gustavo Molina
Products that bear the label "natural" have gained more attention in the marketplace. In this approach, the production of aroma compounds through biotransformation or bioconversion has been receiving more incentives in economic and research fields. Among the substrates used in these processes, terpenes can be highlighted for their versatility and low cost; some examples are limonene, α-pinene, and β-pinene. This work focused on the biotransformation of the two bicyclic monoterpenes, α-pinene and β-pinene; the use of different biocatalysts; the products obtained; and the conditions employed in the process...
January 20, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28105486/immobilization-of-alkaline-polygalacturonate-lyase-from-bacillus-subtilis-on-the-surface-of-bacterial-polyhydroxyalkanoate-nano-granules
#5
GanQiao Ran, Dan Tan, WeiEr Dai, XinLiang Zhu, JiPing Zhao, Qi Ma, XiaoYun Lu
Alkaline polygalacturonate lyase (PGL), one of the pectinolytic enzymes, has been widely used for the bioscouring of cotton fibers, biodegumming, and biopulp production. In our study, PGL from Bacillus subtilis was successfully immobilized on the surface of polyhydroxyalkanoate (PHA) nanogranules by fusing PGL to the N-terminal of PHA synthase from Ralstonia eutropha via a designed linker. The PGL-decorated PHA beads could be simply achieved by recombinant fermentation and consequent centrifugation. The fused PGL occupied 0...
January 20, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28105485/biochemical-characterization-of-thermostable-%C3%AE-1-4-mannanase-belonging-to-the-glycoside-hydrolase-family-134-from-aspergillus-oryzae
#6
Kiyota Sakai, Mai Mochizuki, Miyuki Yamada, Yuta Shinzawa, Miho Minezawa, Saran Kimoto, Shunsuke Murata, Yuhei Kaneko, Saaya Ishihara, Sadanari Jindou, Tetsuo Kobayashi, Masashi Kato, Motoyuki Shimizu
A β-1,4-mannanase, termed AoMan134A, that belongs to the GH 134 family was identified in the filamentous fungus Aspergillus oryzae. Recombinant AoMan134A was expressed in Pichia pastoris, and the purified enzyme produced mannobiose, mannotriose, mannotetraose, and mannopentaose from galactose-free β-mannan, with mannotriose being the predominant reaction product. The catalytic efficiency (k cat/K m ) of AoMan134A was 6.8-fold higher toward galactomannan from locust bean gum, than toward galactomannan from guar gum, but similar toward galactomannan from locust bean gum and glucomannan from konjac flour...
January 19, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28102432/synthesis-of-human-parainfluenza-virus-4-nucleocapsid-like-particles-in-yeast-and-their-use-for-detection-of-virus-specific-antibodies-in-human-serum
#7
Aistė Bulavaitė, Rita Lasickienė, Paulius Lukas Tamošiūnas, Martynas Simanavičius, Kęstutis Sasnauskas, Aurelija Žvirblienė
The aim of this study was to produce human parainfluenza virus type 4 (HPIV4) nucleocapsid (N) protein in yeast Saccharomyces cerevisiae expression system, to explore its structural and antigenic properties and to evaluate its applicability in serology. The use of an optimized gene encoding HPIV4 N protein amino acid (aa) sequence GenBank AGU90031.1 allowed high yield of recombinant N protein forming nucleocapsid-like particles (NLPs) in yeast. A substitution L332D disrupted self-assembly of NLPs, confirming the role of this position in the N proteins of Paramyxovirinae...
January 19, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28101612/abolishment-of-n-glycan-mannosylphosphorylation-in-glyco-engineered-saccharomyces-cerevisiae-by-double-disruption-of-mnn4-and-mnn14-genes
#8
Yeong Hun Kim, Ji-Yeon Kang, Jin Young Gil, Sang-Yoon Kim, Keun Koo Shin, Hyun Ah Kang, Jeong-Yoon Kim, Ohsuk Kwon, Doo-Byoung Oh
Mannosylphosphorylated glycans are found only in fungi, including yeast, and the elimination of mannosylphosphates from glycans is a prerequisite for yeast glyco-engineering to produce human-compatible glycoproteins. In Saccharomyces cerevisiae, MNN4 and MNN6 genes are known to play roles in mannosylphosphorylation, but disruption of these genes does not completely remove the mannosylphosphates in N-glycans. This study was performed to find unknown key gene(s) involved in N-glycan mannosylphosphorylation in S...
January 18, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28097405/role-of-exopolysaccharide-in-salt-stress-resistance-and-cell-motility-of-mesorhizobium-alhagi-ccnwxj12-2-t
#9
Xiaodong Liu, Yantao Luo, Zhefei Li, Jiamei Wang, Gehong Wei
Mesorhizobium alhagi, a legume-symbiont soil bacterium that forms nodules with the desert plant Alhagi sparsifolia, can produce large amounts of exopolysaccharide (EPS) using mannitol as carbon source. However, the role of EPS in M. alhagi CCNWXJ12-2(T), an EPS-producing rhizobium with high salt resistance, remains uncharacterized. Here, we studied the role of EPS in M. alhagi CCNWXJ12-2(T) using EPS-deficient mutants constructed by transposon mutagenesis. The insertion sites of six EPS-deficient mutants were analyzed using single primer PCR, and two putative gene clusters were found to be involved in EPS synthesis...
January 17, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28097404/complete-mitochondrial-genome-of-the-endophytic-fungus-pestalotiopsis-fici-features-and-evolution
#10
Shu Zhang, Xiu-Na Wang, Xiao-Ling Zhang, Xing-Zhong Liu, Yong-Jie Zhang
Endophytic fungi (EF) live within plants and have profound impacts on plant communities. They are astonishingly diverse but poorly studied at the genome level. Herein, we assembled the mitochondrial genome (mitogenome) of the EF Pestalotiopsis fici, annotated and compared it with those of other relatives to better understand the evolution of the EF lineage. Except for standard fungal mitochondrial genes, the 69,529-bp circular mitogenome of P. fici harbors 18 introns acquired possibly through lateral transfer from other fungi and nine free-standing open reading frames with some scarcely seen in fungal mitogenomes...
January 17, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28093622/quantification-of-chloroflexi-eikelboom-morphotype-1851-for-prediction-and-control-of-bulking-events-in-municipal-activated-sludge-plants-in-japan
#11
Tadashi Nittami, Lachlan B M Speirs, Takuya Yamada, Ichiro Suzuki, Junji Fukuda, Futoshi Kurisu, Robert J Seviour
The dominant filamentous bacteria associated with bulking incidents in Japanese activated sludge plants with nutrient removal were identified and their quantitative correlations with sludge settleability were assessed, with the aim of controlling bulking incidents by specifically suppressing bacterial growth. Fluorescence in situ hybridization (FISH) analyses using existing oligonucleotide FISH probes indicated that the presence of Eikelboom type 1851 filamentous bacteria belonging to the phylum Chloroflexi is correlated with biomass settleability in the municipal wastewater treatment plants examined...
January 17, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28091792/restoration-using-azolla-imbricata-increases-nitrogen-functional-bacterial-groups-and-genes-in-soil
#12
Xiao-Ming Lu, Peng-Zhen Lu, Ke Yang
Microbial groups are major factors that influence soil function. Currently, there is a lack of studies on microbial functional groups. Although soil microorganisms play an important role in the nitrogen cycle, systematic studies of the effects of environmental factors on microbial populations in relation to key metabolic processes in the nitrogen cycle are seldom reported. In this study, we conducted a systematic analysis of the changes in nitrogen functional groups in mandarin orange garden soil treated with Azolla imbricata...
January 16, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28091791/a-novel-scalable-robust-downstream-process-for-oncolytic-rat-parvovirus-isoelectric-point-based-elimination-of-empty-particles
#13
Barbara Leuchs, Veronika Frehtman, Markus Riese, Marcus Müller, Jean Rommelaere
The rodent protoparvovirus H-1PV, with its oncolytic and oncosuppressive properties, is a promising anticancer agent currently under testing in clinical trials. This explains the current demand for a scalable, good manufacturing practice-compatible virus purification process yielding high-grade pure infectious particles and overcoming the limitations of the current system based on density gradient centrifugation. We describe here a scalable process offering high purity and recovery. Taking advantage of the isoelectric point difference between full and empty particles, it eliminates most empty particles...
January 14, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28091790/chemically-modified-surface-functional-groups-of-alcaligenes-sp-s-xj-1-to-enhance-its-demulsifying-capability
#14
Yuyan Zhang, Jia Liu, Xiangfeng Huang, Lijun Lu, Kaiming Peng
Cell-surface functional groups (amino, carboxyl, hydroxyl, as well as phosphate) were chemically modified in various ways to enhance the demulsification capability of the demulsifying bacteria Alcaligenes sp. S-XJ-1. Results demonstrated that the demulsifying activity was significantly inhibited by amino enrichment with cetyl trimethyl ammonium bromide, amino methylation, hydroxyl acetylation, and phosphate esterification, but was gradually promoted by carboxyl blocking with increasing the extents of esterification...
January 14, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28091789/comparative-investigation-on-a-hexane-degrading-strain-with-different-cell-surface-hydrophobicities-mediated-by-starch-and-chitosan
#15
Dong-Zhi Chen, Ning-Xin Jiang, Jie-Xu Ye, Zhuo-Wei Cheng, Shi-Han Zhang, Jian-Meng Chen
Bioremediation usually exhibits low removal efficiency toward hexane because of poor water solubility, which limits the mass transfer rate between the substrate and microorganism. This work aimed to enhance the hexane degradation rate by increasing cell surface hydrophobicity (CSH) of the degrader, Pseudomonas mendocina NX-1. The CSH of P. mendocina NX-1 was manipulated by treatment with starch and chitosan solution of varied concentrations, reaching a maximum hydrophobicity of 52%. The biodegradation of hexane conformed to the Haldane inhibition model, and the maximum degradation rate (ν max) of the cells with 52% CSH was 0...
January 14, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28091788/new-insights-into-the-role-of-ph-and-aeration-in-the-bacterial-production-of-calcium-carbonate-caco3
#16
Mostafa Seifan, Ali Khajeh Samani, Aydin Berenjian
Over recent years, the implementation of microbially produced calcium carbonate (CaCO3) in different industrial and environmental applications has become an alternative for conventional approaches to induce CaCO3 precipitation. However, there are many factors affecting the biomineralization of CaCO3, which may restrict its application. In this study, we investigated the effects of pH and aeration as the main two influential parameters on bacterial precipitation of CaCO3. The results showed that the aeration had a significant effect on bacterial growth and its rise from 0...
January 13, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28091787/production-of-water-soluble-yellow-pigments-via-high-glucose-stress-fermentation-of-monascus-ruber-cgmcc-10910
#17
Meihua Wang, Tao Huang, Gong Chen, Zhenqiang Wu
Monascus pigments are secondary metabolites of Monascus species and are mainly composed of yellow pigments, orange pigments and red pigments. In this study, a larger proportion of Monascus yellow pigments could be obtained through the selection of the carbon source. Hydrophilic yellow pigments can be largely produced extracellularly by Monascus ruber CGMCC 10910 under conditions of high glucose fermentation with low oxidoreduction potential (ORP). However, keeping high glucose levels later in the culture causes translation or a reduction of yellow pigment...
January 13, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28084539/mcra-primers-for-the-detection-and-quantification-of-the-anaerobic-archaeal-methanotroph-candidatus-methanoperedens-nitroreducens
#18
Annika Vaksmaa, Mike S M Jetten, Katharina F Ettwig, Claudia Lüke
The nitrogen and methane cycles are important biogeochemical processes. Recently, 'Candidatus Methanoperedens nitroreducens,' archaea that catalyze nitrate-dependent anaerobic oxidation of methane (AOM), were enriched, and their genomes were analyzed. Diagnostic molecular tools for the sensitive detection of 'Candidatus M. nitroreducens' are not yet available. Here, we report the design of two novel mcrA primer combinations that specifically target the alpha sub-unit of the methyl-coenzyme M reductase (mcrA) gene of 'Candidatus M...
January 13, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28084538/mainstream-partial-nitritation-anammox-in-municipal-wastewater-treatment-status-bottlenecks-and-further-studies
#19
REVIEW
Yeshi Cao, Mark C M van Loosdrecht, Glen T Daigger
Driven by energy neutral/positive of wastewater treatment plants, significant efforts have been made on the research and development of mainstream partial nitritation and anaerobic ammonium oxidation (anammox) (PN/A) (deammonification) process since the early 2010s. To date, feasibility of mainstream PN/A process has been demonstrated and proven by experimental results at various scales although with the low loading rates and elevated nitrogen concentration in the effluent at low temperatures (15-10 °C). This review paper provides an overview of the current state of research and development of mainstream PN/A process and critically analyzes the bottlenecks for its full-scale application...
January 13, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28083652/improved-eicosapentaenoic-acid-production-in-pythium-splendens-rbb-5-based-on-metabolic-regulation-analysis
#20
Liang Ren, Pengpeng Zhou, Yuanmin Zhu, Ruijiao Zhang, Longjiang Yu
Eicosapentaenoic acid (EPA) is an essential polyunsaturated fatty acid for human beings. At present, the production of commercially available long-chain polyunsaturated fatty acids, mainly from wild-caught ocean fish, is struggling to meet the increasing demand for EPA. Production of EPA by microorganisms may be an alternative, effective and economical method. The oleaginous fungus Pythium splendens RBB-5 is a potential source of EPA, and thanks to the simple culture conditions required, high yields can be achieved in a facile manner...
January 12, 2017: Applied Microbiology and Biotechnology
journal
journal
28803
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"