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Acta Veterinaria Hungarica

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https://www.readbyqxmd.com/read/29958531/electroretinographic-findings-in-day-blind-dogs
#1
Magdalena Drążek-Kubiak, Marcin Lew, Sylwia Lew
Cone degeneration (cd; day blindness) is one of the inherited retinal diseases of dogs. Its diagnosis is based on vision testing, fundoscopy, electroretinography (ERG) and, for some breeds, on genetic tests. Typical signs of the disease are day blindness and cone dysfunction during ERG while fundoscopy does not show any abnormalities. The aim of this study was to compare behavioural findings, fundoscopic lesions and electroretinographic alterations in 12 cd-affected dogs (Alaskan Malamute, Labrador Retriever, German Shepherd, Dachshund, Yorkshire Terrier, Shih Tzu, Siberian Husky and crossbreeds) examined at our clinic...
June 2018: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/29958530/anatomical-and-histological-characteristics-of-the-lungs-in-the-ground-squirrel-spermophilus-citellus
#2
Miloš Blagojević, Ivana Božičković, Gordana Ušćebrka, Olivera Lozanče, Milena Đorđević, Zoran Zorić, Ivana Nešić
The aim of this work was to study the topography, morphology, vascularisation, histology and innervation of the lungs in the ground squirrel (Spermophilus citellus) and compare these data with those concerning the rat, mole rat, rabbit and mouse. The research was carried out on 15 animals. It was revealed that the right lung has four lobes (cranial, middle, caudal and accessory lobes), while the left lung is not divided into segments. The functional vessels are a. pulmonalis dextra et sinistra and vv. pulmonales (5-6), while the nutritive vessels of the lungs are a...
June 2018: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/29958529/tyrosine-kinase-expression-analyses-in-canine-mammary-gland-tumours-a-pilot-study
#3
Zsófia Koltai, Bernadett Szabó, Judit Jakus, Péter Vajdovich
Messenger RNA levels of oncogenic tyrosine kinases were determined in canine mammary tumours using real-time RT-PCR. The following tyrosine kinases and vascular endothelial growth factors (VEGF) were examined in malignant and healthy mammary tissues of 13 dogs: VEGFR1, VEGFR2, EGFR, ErbB2, PDGFR1, c-KIT and c-MET. Expression levels of all these factors were significantly higher in tumour samples than in normal mammary tissues taken from the same animal. Higher grading was associated with higher VEGFR1 levels...
June 2018: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/29958528/glomerulosclerosis-in-transgenic-rabbits-with-ubiquitous-venus-protein-expression
#4
Nándor Lipták, Orsolya Ivett Hoffmann, Gabriella Skoda, Elen Gócza, Andrea Kerekes, Zsuzsanna Bősze, László Hiripi
Focal segmental glomerulosclerosis (FSGS) is a potential cause of nephrotic syndrome both in humans and pet mammals. Glomerulopathy was reported earlier in green fluorescent protein (GFP) transgenic (TG) mice, but glomerulosclerosis has not been examined in GFP TG rabbits so far. In the present study, the potential manifestation of FSGS was investigated in both Venus TG rabbits generated by Sleeping Beauty (SB) transposition and age-matched control New Zealand White (NZW) rabbits. Venus protein fluorescence was detected by confocal microscopy and quantified by microplate reader...
June 2018: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/29958527/assessment-of-corneal-and-conjunctival-metaplasia-by-impression-cytology-during-the-treatment-of-canine-keratoconjunctivitis-sicca
#5
Krzysztof Radziejewski, Ireneusz Balicki, Mateusz Szadkowski
The purpose of this study was to assess the condition of cells in the conjunctiva and corneal epithelium prior to and during cyclosporine- or tacrolimusbased treatment of keratoconjunctivitis sicca (KCS). The study was performed on 40 dogs with KCS. The dogs were divided into two groups of 20 animals each. In Group I, 0.75% cyclosporine eye drops were administered three times a day, while in Group II 0.02% tacrolimus eye drops were administered twice daily. Additionally, each group was subdivided into three subgroups based on the results of the Schirmer I tear test (STT I)...
June 2018: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/29958526/detection-of-pregnancy-in-sheep-using-an-elisa-for-pregnancy-specific-protein-b
#6
Tímea Milisits-Németh, Orsolya Gabriella Balogh, István Egerszegi, László Kern, R Garth Sasser, György Gábor
The early detection of pregnancy and the determination of fetal numbers have economic benefits in sheep production because of the seasonal breeding patterns where missing a breeding opportunity means the loss of one productive year. The purpose of this study was to evaluate the efficacy of the B6-HRP ELISA for ovine pregnancy-specific protein B (oPSPB) measurement in the detection of pregnancy and estimation of fetal numbers in different sheep breeds. BioPRYN® ELISA assay kit was used for the detection of pregnancy in the experimental animals...
June 2018: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/29958525/molecular-epidemiology-of-brucella-melitensis-strains-causing-outbreaks-in-croatia-and-bosnia-and-herzegovina
#7
Sanja Duvnjak, Ivana Račić, Silvio Špičić, Maja Zdelar-Tuk, Irena Reil, Željko Cvetnić
The most recent data on the incidence of brucellosis in Southeast Europe prove the persistence of this zoonosis in the area, regardless of constant efforts at controlling it as one of the most dangerous zoonoses. Forty-three Brucella melitensis strains were collected from cattle, sheep, goats and humans from Croatia as well as Bosnia and Herzegovina between 2009 and 2015. The strains were identified and genotyped in order to determine their epidemiological background. Standard biotyping methods and Bruce-ladder were used to identify the strains...
June 2018: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/29958524/evaluation-of-pgp-mdr1-immunohistochemistry-in-canine-lymphoma-prognostic-and-clinical-aspects
#8
Péter Vajdovich, Zsófia Koltai, Valéria Dékay, Krisztina Kungl, Andrea Harnos
Permeability glycoprotein (P-glycoprotein, Pgp) immunohistochemistry (IHC) was evaluated in dogs with multicentric lymphoma treated with cyclophosphamide- doxorubicin-vincristine-prednisolone with or without L-Asparaginase. Lymph nodes of 33 untreated dogs were immunophenotyped: Ki67% and Pgp analyses (with anti-Pgp, monoclonal mouse C494 clone) were performed. Pgp positivity rate and intensity were determined microscopically (by manual counting done by two blinded authors in two parallel specimens). The median overall survival time (OST) was 333 days and the relapse-free period (RFP) 134 days...
June 2018: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/29958523/myxobolus-infection-in-the-cornea-of-the-roach-rutilus-rutilus-in-lake-balaton
#9
Réka Borzák, Kálmán Molnár, Gábor Cech, Csaba Székely
Infection of the cornea in fishes by Myxobolus plasmodia is a common but still little known site preference of myxosporeans. A sporadic but striking infection in the cornea of the roach (Rutilus rutilus) was observed in Lake Balaton, Hungary. Relatively small, round plasmodia 250 to 500 μm in diameter developed in the dense connective tissue of the cornea. Morphological and molecular biological examination of spores collected from cysts in the cornea demonstrated that this infection is caused by Myxobolus fundamentalis, a species hitherto reported only from the cartilaginous gill arch of the roach...
June 2018: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/29958522/cross-section-of-gill-filaments-in-histological-preparations-helps-better-identification-of-the-location-of-myxosporean-plasmodia-in-gill-tissues
#10
Kálmán Molnár, Ádám Varga, Csaba Székely
Location and tissue preference of filamental-type myxosporean plasmodia in histological slides of the gills can be properly identified only in cross sections of the gill filaments. The authors selected three myxosporeans (Myxobolus rutili, M. dispar and Henneguya psorospermica, parasites of the roach, the common carp and the pike, respectively) for studying the problem. The plasmodia of these species studied in longitudinal sections were earlier regarded as developing inside the filamental arteries. Cross sections of the filaments showed that all the three species developed plasmodia in the dense connective tissue constituting the adventitia of gill arteries and covering the cartilaginous gill rays...
June 2018: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/29958521/hippocampal-necrosis-and-sclerosis-in-cats-a-retrospective-study-of-35-cases
#11
Andrea Klang, Sandra Högler, Nora Nedorost, Christiane Weissenbacher-Lang, Ákos Pákozdy, Bethan Lang, Herbert Weissenböck
Hippocampal necrosis and hippocampal sclerosis in cats is a neuropathological entity which is a major concern in feline epilepsy. The aim of our study was to identify associated pathologic brain lesions possibly serving as aetiological triggers in this condition. Therefore, the formalin-fixed and paraffin waxembedded brain tissue of 35 cats diagnosed with hippocampal necrosis or sclerosis was examined retrospectively. In 26 cats inflammatory infiltrates could be found in the hippocampus or adjacent brain regions...
June 2018: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/29958520/myxidium-shedkoae-sokolov-2013-myxozoa-myxidiidae-a-parasite-of-the-gallbladder-of-perccottus-glenii-dybowski-1877-actinoptrygii-odontobutidae-supplementary-data-on-morphology-and-phylogenetic-position-based-on-18s-rdna-sequence-analysis
#12
Sergey Sokolov, Daria Lebedeva
This paper is the first report on the molecular characterisation of myxozoan parasites from the odontobutid fish Chinese (Amur) sleeper (Perccottus glenii Dybowski, 1877). The authors determined the partial 18S rDNA sequence of Myxidium shedkoae Sokolov, 2013 from the gallbladder of the fish. Phylogenies reconstructed using maximum likelihood and Bayesian inference analysis revealed that M. shedkoae belongs to the hepatic biliary group of myxozoans (after Kristmundsson and Freeman, 2013) as a member of the clade consisting of Zschokkella sp...
June 2018: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/29958519/variations-in-the-detection-of-anti-pedv-antibodies-in-serum-samples-using-three-diagnostic-tests-short-communication
#13
Jan Plut, Ivan Toplak, Marina Štukelj
Over the last few years several porcine epidemic diarrhoea (PED) outbreaks have been discovered in Europe including the first PED case in Slovenia in January 2015. The aim of this study was to determine when PED virus (PEDV) infection started in Slovenia. Serum samples collected between 2012 and 2016 were tested. Three hundred and seventy-five serum samples were collected from 132 Slovenian small, one-site pig farms. Samples were tested for PEDV antibodies utilising three different serological methods: commercially-available indirect ELISA, in-house blocking ELISA test and Immunoperoxidase Monolayer Assay (IPMA) test...
June 2018: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/29958518/analysis-of-the-immune-response-of-calves-to-various-saponin-based-adjuvants-for-an-experimental-mycoplasma-bovis-vaccine
#14
Katarzyna Dudek, Dariusz Bednarek, Roger D Ayling, Maria Szczotka, Ewelina Iwan, Janusz Kocki
Mycoplasma bovis is a primary infectious agent of many disorders in cattle including bovine respiratory disease. No commercial vaccines against M. bovis are available in Europe. The immune response of calves to three saponin-based adjuvants combined with a field Polish M. bovis strain was evaluated. Four groups of six calves each were injected subcutaneously with the M. bovis strain combined with either saponin, saponin + Emulsigen®, saponin + Emulsigen® + alphatocopherol acetate, or with phosphate-buffered saline as control group...
June 2018: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/29958517/effect-of-single-nucleotide-polymorphisms-on-the-breeding-value-of-fertility-and-breeding-value-of-beef-in-hungarian-simmental-cattle
#15
István Anton, Balázs Húth, Imre Füller, György Gábor, Gabriella Holló, Attila Zsolnai
The objective of this study was to estimate the effect of single-nucleotide polymorphisms (SNPs) on the breeding value of fertility (BVF) and the breeding value of beef (BVB) in Hungarian Simmental cattle. Genotypes were determined on a high-density Illumina Bovine DNA Chip. Data screening and data identification were performed by multi-locus mixed-model. Statistical analyses were carried out to find associations between individual genotypes and the investigated quality values. Three loci showed considerable association with BVF (-log10 P = 9...
June 2018: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/29580090/helminths-of-european-smelt-osmerus-eperlanus-linnaeus-1758-in-lake-ha%C3%A5-cza-and-the-vistula-lagoon-with-special-regard-to-their-zoonotic-threats
#16
Janina Dziekońska-Rynko, Katarzyna Mierzejewska, Katarzyna Kubiak, Martyna Rydzewska, Piotr Hliwa
Reports published in recent years on the increased risk in the Baltic Sea of fish infection with larvae of nematodes potentially pathogenic to humans have prompted a study of European smelt Osmerus eperlanus with respect to the presence of these parasites in the Vistula Lagoon. Additionally, samples of this fish from Lake Hańcza were comparatively considered. The body cavity, the surface of internal organs, the alimentary tract and the swimbladder of fish from two environmentally different water bodies were taken into account in the analysis...
March 2018: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/29580089/detection-and-molecular-characterisation-of-ehrlichia-canis-in-naturally-infected-dogs-in-south-west-nigeria
#17
Olukayode Olugbenga Daramola, Michael Irewole Takeet, Ibironke Kofoworola Oyewusi, Mufutau Atanda Oyekunle, Adewale Oladele Talabi
Canine ehrlichiosis is an important tick-borne rickettsial disease mainly caused by Ehrlichia canis. This study aimed to detect and characterise E. canis in dogs in Abeokuta, Nigeria by microscopy and nested PCR. Blood samples were collected from 205 dogs, thin smears were made, field-stained, and DNA was extracted from the blood samples. A partial region of the 16S rRNA gene was amplified by polymerase chain reaction (PCR) and sequenced unidirectionally. Ehrlichial morulae were detected in three dogs (1.5%)...
March 2018: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/29580088/effect-of-fulvic-and-humic-acids-on-copper-and-zinc-homeostasis-in-rats
#18
István Hullár, András Valentin Vucskits, Erzsébet Berta, Emese Andrásofszky, András Bersényi, József Szabó
The objective of this study was to investigate the effects of fulvic acid (FA) and humic acid (HA), the two main compounds of humic substances (HSs), on copper (Cu) and zinc (Zn) homeostasis. Seventy-two male Wistar rats were randomly divided into nine experimental groups. The control diet (AIN-93G formula) and the diets supplemented with 0.1%, 0.2%, 0.4% and 0.8% FA or HA were fed for 26 days. Cu and Zn concentrations of the large intestinal content (LIC), liver, kidney, femur and hair were determined. FA and HA did not influence significantly the Cu or Zn contents of the experimental diets, the rats' feed intake, weight gain and the feed to gain ratio...
March 2018: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/29580086/short-term-effects-of-t-2-toxin-or-deoxynivalenol-on-glutathione-status-and-expression-of-its-regulatory-genes-in-chicken
#19
RANDOMIZED CONTROLLED TRIAL
Mangesh Nakade, Csilla Pelyhe, Benjámin Kövesi, Krisztián Balogh, Balázs Kovács, Judit Szabó-Fodor, Erika Zándoki, Miklós Mézes, Márta Erdélyi
Short-term (48-hour) effects of 3.74/1.26 mg kg-1 T-2/HT-2 toxin or 16.12 mg kg-1 DON in feed were investigated in the liver of three-week-old cockerels (body weight: 749.60 ± 90.98 g). Markers of lipid peroxidation showed no significant changes. At hour 24, glutathione content in the T-2/HT-2 toxin group was significantly higher than in the control. Glutathione peroxidase activity was significantly higher than the control at hour 24 in the T-2/H-2 toxin group and at hour 48 in the DON group. In the DON group, expression of the glutathione peroxidase 4 gene (GPX4) was significantly lower than in the control at hours 12 and 14, and higher at hour 48...
March 2018: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/29580085/monitoring-minimal-residual-disease-in-canine-lymphomas-treated-with-modified-l-cop-or-l-chop-protocols
#20
Sirintra Sirivisoot, Patharakrit Teewasutrakul, Somporn Techangamsuwan, Sirikachorn Tangkawattana, Anudep Rungsipipat
Heteroduplex polymerase chain reaction for antigen receptor rearrangements (hPARR) was developed to monitor minimal residual disease (MRD) in canine B- and T-cell lymphomas treated with the modified L-COP or L-CHOP protocol. Thirty-five dogs were recruited in this study and their neoplastic lineages were determined by immunophenotyping with Pax5 and CD3. Peripheral blood leukocytes were collected prior to and during chemotherapy in weeks 4, 9 and 13 to detect MRD by hPARR. Twenty-eight dogs (80%) had B-cell lymphoma while seven dogs (20%) had T-cell lymphoma...
March 2018: Acta Veterinaria Hungarica
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