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Acta Veterinaria Hungarica

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https://www.readbyqxmd.com/read/27653436/demonstration-of-homologous-recombination-events-in-the-evolution-of-bovine-viral-diarrhoea-virus-by-in-silico-investigations
#1
Csaba Kővágó, Ákos Hornyák, Violetta Kékesi, Miklós Rusvai
Complete genome sequences of bovine viral diarrhoea virus types 1 and 2 (BVDV-1 and 2) deposited in the GenBank were submitted to bioinformatic analysis using a recombination-detecting software. The results indicate that recombination events are not rare in the case of BVDV, which frequently causes immunotolerance and, consequently, persistent infection in calves. The lack of specific immunity provides an ideal possibility for multiple infections by antigenically related but genetically different BVDV strains, and hence recombinations may occur...
September 2016: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/27653435/t-2-mycotoxin-slows-down-the-development-of-mouse-blastocysts-decreases-their-blastomere-number-and-increases-chromatin-damage
#2
Bence Somoskői, Melinda Kovács, Sándor Cseh
The mycotoxin T-2 has many harmful effects on mammalian cells and reproductive functions. In the present study, the in vitro effect of T-2 toxin on mouse blastocysts was examined. Embryos were cultured in media supplemented with 0.5, 0.75 and 1 ng/ml T-2. Different exposure times were applied [96 h (treatment I) or 24 h following 72 h in toxin-free media (treatment II)]. Blastomere number, nuclear chromatin status and blastocoel formation were investigated in blastocysts. Our data show that the effect of T-2 toxin may vary depending on the stage of the embryo at the start of exposure...
September 2016: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/27653434/correlation-of-carcass-meat-content-with-development-of-the-reproductive-system-in-sexually-immature-gilts
#3
Hanna Jankowiak, Wojciech Kapelański, Maria Bocian, Angéla Soltész, Péter Balogh
This study evaluated the correlation of carcass meat content with the development of the reproductive system in sexually immature gilts of Polish Large White (PLW) and Polish Landrace (PL) breeds. The results, obtained from three groups (I, II, III) of gilts differing in lean meat percentage, indicate an effect on fattening and slaughtering traits as well as on the morphometric characteristics of the uterus. There were statistically significant differences in the values of fattening and slaughtering traits in the groups of gilts tested...
September 2016: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/27653433/quick-cytogenetic-screening-of-breeding-bulls-using-flow-cytometric-sperm-dna-histogram-analysis
#4
Szabolcs Nagy, Péter J Polgár, Magnus Andersson, András Kovács
The aim of the present study was to test the FXCycle PI/RNase kit for routine DNA analyses in order to detect breeding bulls and/or insemination doses carrying cytogenetic aberrations. In a series of experiments we first established basic DNA histogram parameters of cytogenetically healthy breeding bulls by measuring the intraspecific genome size variation of three animals, then we compared the histogram profiles of bulls carrying cytogenetic defects to the baseline values. With the exception of one case the test was able to identify bulls with cytogenetic defects...
September 2016: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/27653432/effect-of-elisa-kit-manufacturing-process-and-incubation-time-on-progesterone-concentration-measured-in-dog-serum-for-ovulation-diagnosis-short-communication
#5
Julianna Thuróczy, Jenő Reiczigel, Lajos Balogh
Twenty-two serum samples of healthy bitches were tested with the frozen and lyophilised version of the same ELISA kit (Quanticheck, Faculty of Veterinary Science, Budapest, Hungary). Samples were chosen on the basis of their progesterone (P4) concentrations, which were between 1.00 and 20.00 ng/mL. As it is well known, this range has the highest clinical relevance in ovulation diagnosis. Both types of microplates were read at 15-min intervals from the 15th until the 90th minute (min) of incubation, and the results were compared with those of frozen plates at 60 min of incubation as 100 percent...
September 2016: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/27653431/multiple-congenital-cardiovascular-defects-including-type-iv-persistent-truncus-arteriosus-in-a-shetland-pony-short-communication
#6
Marian Taulescu, Chiara Palmieri, Joshua Leach, Andras Nagy, Ciprian Ober, Mihai Cernea, Cornel Catoi
This case report describes the pathological findings of multiple congenital cardiac defects in a 2-year-old female Shetland pony with clinical signs of chronic respiratory distress. Persistent truncus arteriosus (PTA) type IV, interventricular septal defect, overriding aorta, pulmonary trunk agenesis, pulmonary arteries arising from the descending aorta, and compensatory right ventricular hypertrophy were observed.
September 2016: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/27653430/impacts-of-urbanisation-level-and-distance-from-potential-natural-mosquito-breeding-habitats-on-the-abundance-of-canine-dirofilariosis
#7
Attila Trájer, Antal Rengei, Kinga Farkas-Iványi, Ákos Bede-Fazekas
Dirofilariosis is an emerging mosquito-borne veterinary and medical problem in the Northern hemisphere. The ecological investigation of 56 canine dirofilariosis cases in new endemic locations was performed in Szeged, Hungary. The aim was to analyse the influence of the spatial patterns of dog abundance and the potential mosquito breeding habitats on the spatial occurrence patterns of dirofilariosis in the city of Szeged. The limnoecological characterisation was based on the fluvial habitat classification of Amoros of natural water bodies; the built environment was evaluated using the UrbanisationScore urbanisation intensity measuring software...
September 2016: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/27653429/congenital-and-acquired-anomalies-of-the-caudal-vertebrae-in-dogs-radiographic-classification-and-prevalence-evaluation
#8
Michaela Paninárová, Ladislav Stehlík, Pavel Proks, Massimo Vignoli
A prospective study to create radiographic classification of caudal vertebral anomalies in dogs was performed and the prevalence of the disorders estimated. Radiographic examination of the caudal vertebrae was performed in 595 client-owned dogs undergoing routine radiological examination of the hip joints. Anomalies of the caudal vertebrae were divided into four groups: (1) congenital anomalies with aberrant fusion of the vertebral epiphysis, like hemivertebra, block or transitional vertebra; (2) morphological anomalies like vertebra curva and dysplastic vertebral epiphysis; (3) post-traumatic changes, represented by fracture or luxation; and (4) degenerative changes, represented by spondylosis, osteophyte formation and mineralisation of intervertebral spaces...
September 2016: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/27653428/comparative-clinical-evaluation-of-tacrolimus-and-cyclosporine-eye-drops-for-the-treatment-of-canine-keratoconjunctivitis-sicca
#9
Krzysztof Radziejewski, Ireneusz Balicki
The aim of this study was to evaluate the efficacy of tacrolimus eye drops in alleviating the clinical symptoms of canine keratoconjunctivitis sicca (KCS) and to compare this efficacy with that of cyclosporine. The clinical study was conducted on 40 dogs diagnosed with idiopathic KCS. The dogs were divided into two groups of 20 animals each. In Group I, 0.75% cyclosporine eye drops were administered three times a day, while in Group II 0.02% tacrolimus eye drops were given twice daily. In addition, each group was subdivided into three subgroups based on the results of Schirmer tear test I (STT I)...
September 2016: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/27653427/effects-of-subclinical-mycobacterium-avium-ssp-paratuberculosis-infection-on-some-physiological-parameters-health-status-and-production-in-dairy-cows
#10
Viktor Jurkovich, Barbara Bognár, Krisztián Balogh, Mária Kovács-Weber, Kinga Fornyos, Rubina Tünde Szabó, Péter Kovács, László Könyves, Miklós Mézes
Milk yield, milk ingredients, health and other, production-related parameters of subclinically infected, Mycobacterium avium ssp. paratuberculosis (MAP-) shedding (positive faecal PCR, n = 20) and non-shedding (negative faecal PCR, n = 10) dairy cows were compared in the period from 10 days prepartum to 120 days postpartum. Body condition, rumen fill and faeces scores were lower in the MAP-shedding cows. There was no significant difference in plasma or urine metabolic parameters between the groups. Milk yield and lactose content tended to be lower (P = 0...
September 2016: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/27653426/lactobacillus-pentosus-expressing-porcine-lactoferrin-elevates-antibacterial-activity-and-improves-the-efficacy-of-vaccination-against-aujeszky-s-disease
#11
Yigang Xu, Xiaolin Zong, Bing Han, Yijing Li, Lijie Tang
In this study, Lactobacillus pentosus expressing porcine lactoferrin (pLF) was tested for in vitro antibacterial activity and for its ability to enhance immunity induced by an orally administered Aujeszky's disease virus (ADV) vaccine. The cDNA encoding N-terminus of pLF was cloned into a Lactobacillus-specific plasmid to produce L. pentosus pLF expressing transformants (pPG612.1-pLFN/ L. pentosus). The antimicrobial activity of the recombinant pLF protein inhibited bacterial growth in vitro. The supernatant of pPG612...
September 2016: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/27342098/immunological-and-biochemical-characterisation-of-7ap-a-short-protein-translated-from-an-alternative-frame-of-orf7-of-prrsv
#12
Ferenc Olasz, Béla Dénes, Ádám Bálint, Tibor Magyar, Sándor Belák, Zoltán Zádori
Sequence analysis revealed a short alternative open reading frame (ORF) named ORF7a within the nucleocapsid gene of genetically divergent porcine reproductive and respiratory syndrome virus (PRRSV) genomes. Alignment of the corresponding protein sequences (named 7ap) revealed substantial heterogeneity among 7aps of different genotypes, though all of them are predicted to be positively charged. Green fluorescent protein and FLAG fusion constructs of ORF7a of the HU-14432/2011 PRRSV demonstrated that 7ap is expressed...
June 2016: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/27342097/prevalence-of-bovine-viral-diarrhoea-virus-in-cattle-farms-in-hungary
#13
Ágnes Szabára, Zsolt Lang, József Földi, Ákos Hornyák, Tamás Abonyi, László Ózsvári
A study was performed to survey the virological prevalence of bovine viral diarrhoea (BVD) virus (BVDV) in cattle herds in Hungary between 2008 and 2012. A total of 40,413 samples for BVDV detection and 24,547 samples for antibody testing were collected from 3,247 herds (570,524 animals), thus representing approximately 75% of the cattle population in Hungary. Retrospective Bayesian analysis demonstrated that (1) the herd-level true virus prevalence was 12.4%, (2) the mean individual (within-herd) true virus prevalence was 7...
June 2016: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/27342096/molecular-characterisation-of-epizootic-haemorrhagic-disease-virus-associated-with-a-tunisian-outbreak-among-cattle-in-2006
#14
Sameh Ben Dhaou, Corinne Sailleau, Besma Babay, Cyril Viarouge, Soufien Sghaier, Stephan Zientara, Salah Hammami, Emmanuel Bréard
In 2006, epizootic haemorrhagic disease (EHD) outbreaks were recorded in the Maghreb (Tunisia, Morocco and Algeria) among cattle, resulting in severe repercussions on herds (oedema of the head, necrotic lesions of the oral mucosa, hyperthermia of the teats, accompanied by anorexia and respiratory distress) and economic losses. The present study gives new information on the molecular characterisation of the EHD virus (EHDV) that had circulated in Tunisia. Genome segments 2, 3, 6, 7 and 10 of EHDV, corresponding to the VP2, VP3, VP5, VP7 and NS3/NS3A proteins, respectively, were amplified from the blood of one animal by RT-PCR and sequenced...
June 2016: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/27342095/cadmium-induced-changes-in-hatchability-and-in-the-activity-of-aminotransaminases-and-selected-lysosomal-hydrolases-in-the-blood-plasma-of-muscovy-ducklings-cairina-moschata
#15
Małgorzata Dżugan, Marcin Lis
The aim of this study was to determine the effect of cadmium on Muscovy ducklings (Cairina moschata) based on hatching results and the activity of enzymes in the blood plasma. On day 6 of incubation, hatching eggs were injected into the egg albumen with 50 μl of saline solution containing Cd ions (CdCl2) at concentrations of 0 (control group), 1.3, 4.0, 7.5, 15.0 and 30 μg/egg, using 50 eggs per group. A gradual decrease in hatchability, from 52% in the control to 4% in the highest Cd dose group, was observed, with the LD50 calculated as 8 μg/egg...
June 2016: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/27342094/involuntary-reduction-in-vigour-of-calves-born-from-sexed-semen
#16
Radica Djedović, Vladan Bogdanović, Dragan Stanojević, Zsolt Nemes, András Gáspárdy, Sándor Cseh
The objective of this study was to compare the reproductive traits of heifers and the development characteristics of their calves following artificial insemination (AI) with sexed and non-sexed semen. The analysed characteristics included conception rate, gestation length, calf birth weight, calf vigour, stillbirth rate, and twinning rate. Data of 530 calves produced with sexed and 1,163 calves produced with non-sexed semen were analysed. The General Linear Model (GLM) was applied to assess the influence of semen type, farm, season of insemination, the calf's sex and the inseminating sire on gestation length and calf birth weight...
June 2016: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/27342093/omental-leiomyosarcoma-with-unusual-giant-cells-in-a-beagle-dog-short-communication
#17
Jun Sasaki, Megumi Toyoshima, Yasuhiko Okamura, Masanobu Goryo
A 10-year-old castrated male Beagle dog was presented with a 2-month history of intermittent vomiting and abdominal pain. The dog was referred to the Veterinary Teaching Hospital at Iwate University for further evaluation, and a splenic tumour was suspected on the basis of ultrasonography and computed tomography. Surgery identified a large, solid, light-pink mass on the greater omentum with blood-coloured ascites in the abdominal cavity, and resection was performed. Microscopically, the mass comprised spindle-shaped tumour cells and scattered osteoclast-like giant cells...
June 2016: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/27342092/molecular-genetic-investigations-on-balantidium-ctenopharyngodoni-chen-1955-a-parasite-of-the-grass-carp-ctenopharyngodon-idella
#18
Szilvia Marton, Krisztián Bányai, Barbara Forró, György Lengyel, Csaba Székely, Ádám Varga, Kálmán Molnár
Balantidium ctenopharyngodoni is a common ciliate in Hungary, infecting the hindgut of grass carp (Ctenopharyngodon idella), a cyprinid fish of Chinese origin. Although data have already been presented on its occasional pathogenic effect on the endothelium of the host, generally it is a harmless inhabitant of the gut. Phylogenetic analysis of the 18S rDNA and ITS fragments of this protozoan proved that it is in the closest phylogenetic relationship with endocommensalist and symbiont ciliates of mammals feeding on large volumes of green forage, in a similar way as Balantidium spp...
June 2016: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/27342091/histological-investigation-on-ancyrocephalus-paradoxus-dactylogyridea-ancyrocephalidae-infection-causing-mortalities-in-an-intensively-cultured-pikeperch-sander-lucioperca-l-stock
#19
Kálmán Molnár, Gábor Szilágyi, Gábor Mosonyi, Ádám Varga, Csaba Székely
In a cultured pikeperch (Sander lucioperca) stock the monopisthocotylean monogenean gill parasite Ancyrocephalus paradoxus caused heavy infection and mortalities. The gills of the affected fish specimens were infected by 50 to 800 monogenean parasites. Severe pathological changes were found in areas where the worms attached to the gills. At the attachment sites the haptoral discs of the worms formed a deep depression in the epithelium of the filaments, and the anchors pierced into and fixed themselves to the connective tissue of the cartilaginous gill rays...
June 2016: Acta Veterinaria Hungarica
https://www.readbyqxmd.com/read/27342090/application-of-a-combined-global-positioning-and-heart-rate-monitoring-system-in-jumper-horses-during-an-official-competition-a-preliminary-study
#20
Marilena Bazzano, Elisabetta Giudice, Maria Rizzo, Fulvio Congiu, Alessandro Zumbo, Francesca Arfuso, Simona Di Pietro, Daniele Bruschetta, Giuseppe Piccione
The aim of this study was to investigate whether a combined global positioning system (GPS)/heart rate (HR) monitoring system is a valuable tool to assess, step by step, the physiological response of HR and its relationship with speed in healthy horses competing in an official show jumping class. Six mares performing a standardised warm-up and jumping course were monitored using a HR/GPS device. Venous blood lactate (BL), assessed before and after exercise, showed a significant increase (P = 0.0004) following the physical effort...
June 2016: Acta Veterinaria Hungarica
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