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Acta Biologica Hungarica

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https://www.readbyqxmd.com/read/28322092/physico-chemical-changes-in-karkade-hibiscus-sabdariffa-l-seedlings-responding-to-salt-stress
#1
Abdelnasser Galal
Salinity is one of the major abiotic stress factors affecting series of morphological, physiological, metabolic and molecular changes in plant growth. The effect of different concentrations (0, 25, 50, 100 and 150 mM) of NaCl on the vegetative growth and some physiological parameters of karkade (Hibiscus sabdariffa var. sabdariffa) seedling were investigated. NaCl affected the germination rate, delayed emergence and retarded vegetative growth of seedlings. The length of seedling as well as the leaf area was significantly reduced...
March 2017: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28322091/jasmonic-and-salicylic-acids-enhanced-phytochemical-production-and-biological-activities-in-cell-suspension-cultures-of-spine-gourd-momordica-dioica-roxb
#2
Ill-Min Chung, Kaliyaperumal Rekha, Govindasamy Rajakumar, Muthu Thiruvengadam
In vitro cell suspension culture was established for the production of commercially valuable phytochemicals in Momordica dioica. The influence of elicitors in jasmonic acid (JA) and salicylic acid (SA) increased their effect on phytochemical production and biomass accumulation in M. dioica. The results indicate that compared with non-elicited cultures, JA- and SA-elicited cell suspension cultures had significantly enhanced phenolic, flavonoid, and carotenoid production, as well as antioxidant, antimicrobial, and antiproliferative activities...
March 2017: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28322090/the-chemical-inducer-bth-benzothiadiazole-and-root-colonization-by-mycorrhizal-fungi-glomus-spp-trigger-resistance-against-white-rot-sclerotinia-sclerotiorum-in-sunflower
#3
Rita Bán, Gellért Baglyas, Ferenc Virányi, Balázs Barna, Katalin Posta, József Kiss, Katalin Körösi
White rot caused by Sclerotinia sclerotiorum (SS) is one of the most devastating plant diseases of sunflower. Controlling this pathogen by available tools hardly result in acceptable control. The aim of this study was to elucidate the effects of plant resistance inducers, BTH (benzothiadiazole in Bion 50 WG) and arbuscular mycorrhizal fungi (AMF) on disease development of white rot in three sunflower genotypes. Defence responses were characterized by measuring the disease severity and identifying cellular/histological reactions (e...
March 2017: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28322089/biological-changes-of-green-pea-pisum-sativum-l-by-selenium-enrichment
#4
Farzaneh Garousi, Béla Kovács, Éva Domokos-Szabolcsy, Szilvia Veres
Supplement of common fertilizers with selenium (Se) for crop production will be an effective way to produce selenium-rich food and feed. The value of green pea seeds and forages as alternative protein source can be improved by using agronomic biofortification. Therefore, biological changes of green pea (Pisum sativum L.) and influences of inorganic forms of Se (sodium selenite and sodium selenate) at different concentrations on the accumulation of magnesium (Mg) and phosphorus (P) were investigated in greenhouse experiment...
March 2017: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28322088/alpha-tocopherol-dependent-salt-tolerance-is-more-related-with-auxin-synthesis-rather-than-enhancement-antioxidant-defense-in-soybean-roots
#5
Seda Sereflioglu, Burcu Seckin Dinler, Eda Tasci
In this paper, we describe the alleviated effects of alpha-tocopherol (α-T) on oxidative damage and its possible role as a signal transmitter in plants during salt stress. The results show that exogenously applied α-T under salt stress increased root length and weight, but reduced hydrogen peroxide (H2O2), superoxide anion radical (O2(.)-) and malondialdehyde (MDA) content in soybean roots. The proline content was reduced by α-T treatment. Interestingly, endogenous auxin (IAA) level was significantly increased after α-T application as compared to salt stress alone...
March 2017: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28322087/physiological-role-of-exogenous-nitric-oxide-in-improving-performance-yield-and-some-biochemical-aspects-of-sunflower-plant-under-zinc-stress
#6
Samia Ageeb Akladious, Heba Ibrahim Mohamed
The present study was undertaken to examine the possible roles of sodium nitroprusside in protection against oxidative damage due to zinc toxicity in sunflower plants. Physiochemical parameters in sunflower plants exposed to Zn(2+) (100, 200 and 300 mg/kg soil) alone or combined with SNP were measured. The results showed that excess of Zn decreased plant growth, seed yield components and photosynthetic pigments content. On the other hand, Zn stress increased the level of non-enzymatic antioxidants (ascorbic acid and reduced glutathione) and enzymatic antioxidants (superoxide dismutase, ascrobate peroxidase and glutathione reductase), coupled with the appearance of novel protein bands...
March 2017: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28322086/viability-of-sh-sy5y-cells-is-associated-with-purinergic-p2-receptor-expression-alterations
#7
Arda Örçen, Vuslat Yilmaz, Murat Giriş, Uğur Akcan, Erdem Tüzün, Gaye Erten
To investigate the role of metabotrophic purinergic P2Y receptors in neuroblastoma cell survival, expression of P2 receptors by normal mouse (C57BL/6) brain and human neuroblastoma SH-SY5Y cells was investigated by Western blot and real time PCR studies. Viability of SH-SY5Y cells treated with purinergic receptor antagonists suramin and pyridoxal-phosphate-6-azophenyl-2',4'-disulfonate (PPADS) was evaluated by MTT assay and flow cytometry. In the brain samples of C57BL/6 mice, expressions of P2Y4 and P2X7 were significantly reduced, whereas that of P2Y1 was significantly elevated in an age-dependent manner...
March 2017: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28322085/examination-of-the-effect-of-sodium-nitrite-on-gap-junction-function-during-ischaemia-and-reperfusion-in-anaesthetized-dogs
#8
Gottfried Miskolczi, Márton Gönczi, Mária Kovács, Ágnes Végh
It has previously been proved that sodium nitrite, infused prior to coronary artery occlusion or before reperfusion, results in marked antiarrhythmic effect in anaesthetized dogs. We have now examined whether this protection involves the modulation of gap junction (GJ) function by nitric oxide (NO), derived from nitrite administration under ischaemic conditions. Two groups of chloralose and urethane anaesthetized dogs, each containing 13 animals, were subjected to a 25 min period occlusion of the left anterior descending (LAD) coronary artery, followed by reperfusion...
March 2017: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28322084/fine-structure-and-synaptology-of-the-nitrergic-neurons-in-medial-septum-of-the-rat-brain
#9
Katalin Halasy, Balázs Szőke, Gergely Janzsó
The nitrergic neuron population and certain aspects of their connectivity (peptidergic inputs, co-localization with GABA, synaptic target distribution) were studied in the medial septum of the rat brain. The histochemical localization of NADPH diaphorase and immunohistochemical identification of nNOS at light and electron microscopic level was applied. Double-labeling experiments with galanin and leucine enkephalin, moreover the postembedding GABA immunogold staining was also carried out. NADPH diaphorase- and nNOS-immunopositive neurons could be identified inside the borders of medial septum...
March 2017: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28322083/diurnal-variation-of-the-melanin-concentrating-hormone-level-in-the-hypothalamus
#10
Balázs Gerics, Ferenc Szalay, Péter Sótonyi, Veronika Jancsik
Melanin-concentrating hormone (MCH), the neuropeptide produced mainly in the hypothalamus, plays an operative role in regulating food intake and the sleep/wake cycle. Considering that these physiological functions pursue diurnal variations, we checked whether the total hypothalamic MCH level depends on the time of the day. The aggregated MCH peptide content of the whole MCH neuron population was significantly higher at the end of the sleeping period (lights on), than at the end of the active period (lights off)...
March 2017: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28000513/anatomical-and-physiological-background-permitting-spatial-odor-sensation-in-stylommatophoran-molluscs
#11
REVIEW
Tibor Kiss, Nóra Krajcs
Earlier experiments demonstrated that in order to place protracted tentacles and thereby olfactory receptors in an appropriate position for optimal perception of odor stimuli extraordinary complex movements are required. Until recently both large scale tentacle movements and patterned tentacle movements have been attributed to the concerted involvement of the tentacle retractor muscle and muscles of tegumentum. Recently the existence of three novel muscles in the posterior tentacles of Helix has been discovered...
December 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28000512/developmental-role-of-phenylalanine-ammonia-lyase-pal-and-cinnamate-4-hydroxylase-c4h-genes-during-adventitious-rooting-of-juglans-regia-l-microshoots
#12
Monireh Cheniany, Ali Ganjeali
Phenylalanine-ammonia-lyase and cinnamate-4-hydroxylase play important role in the phenylpropanoid pathway, which produces many biologically important secondary metabolites participating in normal plant development. Flavonol quercetin is the main representant of these compounds that has been identified in numerous Juglans spp. In this survey, the developmental expression patterns of PAL and C4H genes during in vitro rooting of two walnut cultivars 'Sunland' and 'Howard' was examined by RT-PCR. To understand the potential role in rooting, the changing pattern of endogenous content of quercetin was also analyzed by HPLC...
December 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28000511/the-effects-of-cadmium-on-the-biochemical-and-physiological-parameters-of-eruca-sativa
#13
Yasemin Ozdener Kompe, Ahmet Sagiroglu
In this study, Eruca sativa (Rocket) seedlings were treated with different cadmium (Cd) concentrations (0, 150, 300 and 450 μg ∙ g(-1)). The effects of Cd on lipid peroxidation, enzymatic (APx, CAT, GPX, SOD) and non-enzymatic antioxidants (total ascorbate, dehydroascorbate, ascorbate, non-protein thiol), fresh and dry masses, water content were determined. Also, Cd content of the leaves and the roots were analysed. The highest cadmium accumulation of leaves was at 450 μg ∙ g(-1) Cd treatment and the accumulation was 2...
December 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28000510/nadph-diaphorase-histochemistry-selectively-stains-peripheral-and-central-sensory-structures-of-lumbricid-earhworms
#14
Zsuzsanna Solt, Andrea Zsombok, Edit Pollák, László Molnár
By means of whole mount NADPH-diaphorase histochemistry the distribution pattern of primary sensory cells (PSC) and the pathway of their central processes in the ventral nerve cord (VNC) ganglia were investigated in the lumbricid earthworms, Eisenia fetida and Lumbricus terrestris. The distribution pattern of the stained structures seemed to be the same in both species investigated. Strong labelling occurred in sensory fibre branches of segmental nerves and in each of the sensory longitudinal axon bundles of VNC ganglia...
December 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28000509/chip-does-it-work-correctly-the-optimization-steps-of-chromatin-immunoprecipitation
#15
Małgorzata Kus-Liśkiewicz
The proteins interaction with DNA is one of the key regulatory elements of many biological processes; including gene transcription, epigenetic modification or cell differentiation. Immunoprecipitation of chromatin; ChIP; is a method used to assess the interaction of the protein with a DNA sequence, and determines the localization of specific locus in the genome. The main steps of this method are fixation, sonication, immunoprecipitation and analysis of DNA. Although the immunoprecipitation assay is a multipurpose tool applied in biochemistry and biotechnology, it requires optimization...
December 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28000508/proteomic-insight-into-the-primycin-fermentation-process-of-saccharomonospora-azurea
#16
Andrea Valasek, Írisz Éva Kiss, István Fodor, Márk Kovács, Péter Urbán, Éva Jámbor, Csaba Fekete, Ildikó Kerepesi
Saccharomonospora azurea SZMC 14600 is a member of the family Pseudonocardiaceae exclusively used for industrial scale production of primycin a large 36-membered non-polyene macrolide lactone antibiotic belonging to the polyketide class of natural products. Even though maximum antibiotic yield has been achieved by empirically optimized two-step fermentation process, little is known about the molecular components and mechanisms underlying the efficient antibiotic production. In order to identify differentially expressed proteins (DEPs) between the pre- and main-fermentation stages of primycin, comparative 2D-PAGE experiments were performed...
December 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28000507/peptaibol-profiles-of-iranian-trichoderma-isolates
#17
Parisa Rahimi Tamandegani, Doustmorad Zafari, Tamás Marik, András Szekeres, Csaba Vágvölgyi, László Kredics
Five Iranian Trichoderma isolates from species T. viride, T. viridescens, T. asperellum, T. longibrachiatum and T. citrinoviride - selected from the Fungal Collection of the Bu Ali Sina University, Hamedan, Iran - were investigated for their peptaibol production. All examined isolates showed remarkable antibacterial activities during the screening of their extracts for peptaibol content with a Micrococcus luteus test culture. HPLC-ESI-IT MS was used for identification and elucidation of the amino acid sequences of peptaibols...
December 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28000506/a-novel-procedure-to-assess-the-non-enzymatic-hydrogen-peroxide-antioxidant-capacity-of-metabolites-with-high-uv-absorption
#18
Kristóf Csepregi, Éva Hideg
Assays assessing non-enzymatic hydrogen peroxide antioxidant capacities are often hampered by the high UV absorption of the sample itself. This is a typical problem in studies using plant extracts with high polyphenol content. Our assay is based on comparing the 405 nm absorption of the product of potassium iodine and hydrogen peroxide in the presence and absence of a putative hydrogen peroxide reactive antioxidant. This method is free of interference with either hydrogen peroxide or antioxidant self-absorption and it is also suitable for high-throughput plate reader applications...
December 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28000505/uv-b-radiation-increases-anthocyanin-levels-in-cotyledons-and-inhibits-the-growth-of-common-buckwheat-seedlings
#19
Henryk Dębski, Magdalena Szwed, WiesŁaw Wiczkowski, Dorota Szawara-Nowak, Natalia Bączek, Marcin Horbowicz
The impact of short-term UV-B treatment on the content of individual flavonoids and photosynthetic pigments in cotyledons and the growth of common buckwheat (Fagopyrum esculentum Moench) seedlings was investigated. Seeds of four common buckwheat cultivars were germinated in darkness over a period of 4 days and acclimatized for 2 days under a 16/8 h light/dark photoperiod at 24/18 °C day/night, and exposure to 100-120 μmol ∙ m(-2) ∙ s(-1) of photosynthetically active radiation (PAR). Seedlings were divided into three batches, including two batches subjected to different doses of UV-B (5 W ∙ m(-2) and 10 W ∙ m(-2), one hour per day) for 5 days, and a control group exposed to PAR only...
December 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28000504/examination-of-secondary-metabolites-and-antioxidant-capacity-of-anthyllis-vulneraria-fuchsia-sp-galium-mollugo-and-veronica-beccabunga
#20
Rita Csepregi, Tímea Bencsik, Nóra Papp
Anthyllis vulneraria L., Fuchsia sp., Galium mollugo L., and Veronica beccabunga L. were selected to analyse the phenolic content and the antioxidant activity by ferric ion reducing antioxidant power (FRAP) and trolox equivalent antioxidant capacity (TEAC) assays. The highest polyphenol, tannin, and flavonoid contents were measured in Fuchsia species (7.40 ± 0.8, 5.62 ± 0.7 and 0.72 ± 0.1 g/100 g dry weight), while the lowest values were detected in Anthyllis vulneraria (0.68 ± 0.02, 0.17 ± 0.03 and 0...
December 2016: Acta Biologica Hungarica
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