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Acta Biologica Hungarica

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https://www.readbyqxmd.com/read/27630055/anti-listerial-effect-of-selected-essential-oils-and-thymol
#1
Erika-Beáta Kerekes, Anita Vidács, Julianna Jenei Török, Csilla Gömöri, Tamás Petkovits, Muthusamy Chandrasekaran, Shine Kadaikunnan, Naiyf S Alharbi, Csaba Vágvölgyi, Judit Krisch
The anti-listerial effect of marjoram, thyme essential oils (EOs) and thymol on Listeria monocytogenes inoculated chicken breast fillets was investigated. Before inoculation the fillets were pretreated by washing or not under running tap water. Inoculated samples were kept at 6 °C for 24 h to allow the growth of L. monocytogenes. After this, the fillets were put in marinating solutions containing salt (5%) and EOs or thymol in MIC/2 concentration established in vitro. Total germ count (TGC) and L. monocytogenes count was monitored on the meat surface and in the marinating solutions following 24 and 48 h storage at 6 °C...
September 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/27630054/d-galactose-catabolism-in-penicillium-chrysogenum-expression-analysis-of-the-structural-genes-of-the-leloir-pathway
#2
Ágota Jónás, Erzsébet Fekete, Zoltán Németh, Michel Flipphi, Levente Karaffa
In this study, we analyzed the expression of the structural genes encoding the five enzymes comprising the Leloir pathway of D-galactose catabolism in the industrial cell factory Penicillium chrysogenum on various carbon sources. The genome of P. chrysogenum contains a putative galactokinase gene at the annotated locus Pc13g10140, the product of which shows strong structural similarity to yeast galactokinase that was expressed on lactose and D-galactose only. The expression profile of the galactose-1-phosphate uridylyl transferase gene at annotated locus Pc15g00140 was essentially similar to that of galactokinase...
September 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/27630053/purification-and-characterization-of-alginate-lyase-from-locally-isolated-marine-pseudomonas-stutzeri-msea04
#3
Ehab A Beltagy, Aliaa El-Borai, Marina Lewiz, Samy A ElAssar
An alginate lyase with high specific enzyme activity was purified from Pseudomonas stutzeri MSEA04, isolated from marine brown algae. The alginate lyase was purified by precipitation with ammonium sulphate, acetone and ethanol individually. 70% ethanol fraction showed maximum specific activity (133.3 U/mg). This fraction was re-purified by anion exchange chromatography DEAE- Cellulose A-52. The loaded protein was separated into 3 peaks. The second protein peak was the major one which contained 48.2% of the total protein recovered and 79...
September 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/27630052/preculturing-effect-of-thidiazuron-on-in-vitro-shoot-multiplication-and-micropropagation-round-in-capparis-decidua-forsk-an-important-multipurpose-plant
#4
Najat A W Bukhari, Iram Siddique, Kahkashan Perveen
An efficient protocol was developed for clonal multiplication of an important shrub: Capparis decidua (Forsk.) Edgew, through in vitro shoot induction and multiplication from nodal explants. Pretreatment of nodal explants in a liquid Murashige and Skoog (MS) medium augmented with various thidiazuron (TDZ) concentrations at relatively high levels (5-100 μM) for different time duration (4, 8, 12 and 16 d), proved a significant approach for in vitro shoot production. After an initial exposure time to TDZ, nodal explants were inoculated onto a MS basal medium devoid of TDZ for further induction and proliferation...
September 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/27630051/determination-of-zinc-oxide-nanoparticles-toxicity-in-root-growth-in-wheat-triticum-aestivum-l-seedlings
#5
Meppaloor G Prakash, Ill Min Chung
The effect of zinc oxide nanoparticles (ZnONPs) was studied in wheat (Triticum aestivum L.) seedlings under in vitro exposure conditions. To avoid precipitation of nanoparticles, the seedlings were grown in half strength semisolid Murashige and Skoog medium containing 0, 50, 100, 200, 400 and 500 mg L(-1) of ZnONPs. Analysis of zinc (Zn) content showed significant increase in roots. In vivo detection using fluorescent probe Zynpyr-1 indicated accumulation of Zn in primary and lateral root tips. All concentrations of ZnONPs significantly reduced root growth...
September 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/27630050/characterization-of-community-structure-of-culturable-endophytic-fungi-in-sweet-cherry-composite-trees-and-their-growth-retarding-effect-against-pathogens
#6
Neda Haddadderafshi, Tímea Borbála Pósa, Gábor Péter, László Gáspár, Márta Ladányi, Károly Hrotkó, Noémi Lukács, Krisztián Halász
Endophytic fungi have the potential to protect their host plants in stress situations. Characterizing the ecology and complex interaction between these endophytes and their host plants is therefore of great practical importance, particularly in horticultural plants. Among horticultural plants, fruit trees form a special category because of their longevity and because they are composites of rootstock and scion, which often belong to different plant species. Here we present the first characterization of culturable endophytic fungal community of sweet cherry...
September 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/27630049/new-insight-into-the-delia-platura-meigen-caused-alteration-in-nutrient-content-of-soybean-glycine-max-l-merill
#7
Helga Egri Bosnyákné, Ildikó Kerepesi, Sándor Keszthelyi
Climate change has brought about an increasing level of seedcorn maggot (Delia platura Meigen, 1826) (Diptera: Anthomyiidae) damage in Hungary. In order to have a more accurate understanding of the effects of these plant injuries induced by the larvae of D. platura, the nutrient content of soybean (Glycine max L. Merill.) was studied. Our results show that the moisture, raw fat, raw fibre, and raw ash content of the batches damaged by D. platura were significantly less in comparison with that of the control samples...
September 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/27630048/schistosoma-mansoni-antiparasitic-effects-of-orally-administered-nigella-sativa-oil-and-or-chroococcus-turgidus-extract
#8
Medhat Ali, Marwa Abou Eldahab, Hoda Anwer Mansour, Ahmed Nigm
Schistosoma mansoni is one of the parasites causing schistosomiasis, a disease which threatens millions of people all over the world. Traditional chemical drugs are not fully effective against schistosomaisis due to the evolving drug resistant worm strains, so exploring new remedies derived from natural products is a good way to fight schistosomiasis. In the present investigation two natural products, Nigella sativa oil and Chroococcus turgidus extract were used separately or in a combination to explore their effect on S...
September 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/27630047/antioxidant-activities-of-protein-hydrolysates-obtained-from-the-housefly-larvae
#9
Huan Zhang, Pan Wang, Ai-Jun Zhang, Xuan Li, Ji-Hong Zhang, Qi-Lian Qin, Yi-Jun Wu
The housefly is an important resource insect and the housefly larvae are ideal source of food additives. The housefly larvae protein hydrolysates were obtained by enzymatic hydrolysis by alcalase and neutral proteinase. Their antioxidant activities were investigated, including the superoxide and hydroxyl radicalscavenging activity, 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, reducing power and metal chelating activity. The antioxidant activities of both hydrolysates increased with their increasing concentrations...
September 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/27630046/antioxidant-defense-system-parameters-in-isolated-fish-hepatocytes-exposed-to-bisphenol-a-effect-of-vitamin-c
#10
Özlem Kaya, Burak Kaptaner
In this study, isolated hepatocytes of pearl mullet (Alburnus tarichi) were exposed to bisphenol A (BPA) at concentrations of 25, 50, 100, and 200 μM for 24 h. Moreover, an in vitro antioxidant concentration of vitamin C (50 μM) was administrated to the culture medium along with the BPA exposures. Next, the antioxidant defense system parameters were analyzed. According to the results, the highest concentration of BPA (200 μM) proved to be severely toxic for the cells. The increased activities of superoxide dismutase (SOD) and glutathione-S-transferase (GST), the fluctuated activities of glutathione peroxidase (GPx), and the decreased content of reduced glutathione (GSH) were compared to the control group after the BPA exposures...
September 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/27165533/chlorinated-organic-compounds-produced-by-fusarium-graminearum
#11
Khayalethu Ntushelo
Fusarium graminearum, a pathogen of wheat and maize, not only reduces grain yield and degrades quality but also produces mycotoxins in the infected grain. Focus has been on mycotoxins because of the human and animal health hazards associated with them. In addition to work done on mycotoxins, chemical profiling of F. graminearum to identify other compounds produced by this fungus remains critical. With chemical profiling of F. graminearum the entire chemistry of this fungus can be understood. The focus of this work was to identify chlorinated compounds produced by F...
June 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/27165532/quantitative-evaluations-of-the-contribution-of-the-excitatory-ionic-conductance-to-repetitive-spiking-in-a-mathematical-model-of-medial-vestibular-nucleus-neurons
#12
Takaaki Shirahata
Medial vestibular nucleus neurons show spontaneous repetitive spiking. This spiking activity was reproduced by a Hodgkin-Huxley-type mathematical model, which was developed in a previous study. The present study performed computer simulations of this model to evaluate the contribution of the excitatory ionic conductance to repetitive spiking. The present results revealed the difference in the influence of the transient sodium, persistent sodium, and calcium conductance on spiking activity. The differences between the present and previous results obtained from other neuronal mathematical models were discussed...
June 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/27165531/effects-of-essential-oil-combinations-on-pathogenic-yeasts-and-moulds
#13
Györgyi Horváth, Julianna Török Jenei, Csaba Vágvölgyi, Andrea Böszörményi, Judit Krisch
Essential oils (EOs) can be used as alternative or complementary antifungal agents against human pathogenic moulds and yeasts. To reduce the effective dose of antimicrobial agents, EOs are combined which can lead to synergistic or additive effect. In this study the anti-yeast and anti-mould activities of selected EOs were investigated, alone and in combinations, against clinical isolates of Candida albicans, C. parapsilosis, Aspergillus fumigatus, A. terreus, Rhizopus microsporus, Fusarium solani and Lichtheimia corymbifera...
June 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/27165530/changes-in-antioxidant-enzymes-activities-and-proline-total-phenol-and-anthocyanine-contents-in-hyssopus-officinalis-l-plants-under-salt-stress
#14
Omolbanin Jahantigh, Farzaneh Najafi, Hassanali Naghdi Badi, Ramazan Ali Khavari-Nejad, Forough Sanjarian
The relationships between salt stress and antioxidant enzymes activities, proline, phenol and anthocyanine contents in Hyssopus officinalis L. plants in growth stage were investigated. The plants were subjected to five levels of saline irrigation water, 0.37 (tap water as control) with 2, 4, 6, 8 and 10 dSm(-1) of saline water. After two months the uniform plants were harvested for experimental analysis. Antioxidant enzymes activities and proline, phenol and anthocyanine contents of the plants were examinated...
June 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/27165529/effect-of-salinity-and-sodicity-stresses-on-physiological-response-and-productivity-in-helianthus-annuus
#15
Fatma Aly Farghaly, Abeer Ahmed Radi, Dalia Ahmed Abdel-Wahab, Afaf Mohamed Hamada
Soil salinity and sodicity (alkalinity) are serious land degradation issues worldwide that are predicted to increase in the future. The objective of the present study is to distinguish the effects of NaCl and Na(2)CO(3) salinity in two concentrations on the growth, lipoxygenase (LOX) activity, membrane integrity, total lipids, yield parameters and fatty acids (FAs) composition of seeds of sunflower cultivar Sakha 53. Plant growth, LOX activity and malondialdehyde (MDA) content were reduced by salts stresses...
June 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/27165528/exogenous-low-dose-hydrogen-peroxide-enhances-drought-tolerance-of-soybean-glycine-max-l-through-inducing-antioxidant-system
#16
Neslihan Saruhan Guler, Necla Pehlivan
Hydrogen peroxide (H(2)O(2)) functions as a signal molecule in plants under abiotic and biotic stress. In this study, the role of exogenous H(2)O(2) in improving drought tolerance in two soybean cultivars (Glycine max L. Merrill) differing in their tolerance to drought was evaluated. Plants were grown in plastic pots with normal irrigation in a phytotron. Four weeks after radicle emergence, either 1 mM H(2)O(2) or distilled water was sprayed as foliar onto the leaves of each plant, after drought stress was applied...
June 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/27165527/differential-allelopathic-expression-of-different-plant-parts-of-achillea-biebersteinii
#17
Saeid Abu-Romman
Achillea biebersteinii (Asteraceae) is a perennial medicinal plant and has a wide distributional range in the Mediterranean region. The present study investigated the inhibitory effects of different plant parts of A. biebersteinii on germination characteristics and seedling growth of wild barley (Hordeum spontaneum). Water extracts were prepared by incubating separately five grams of dried powder of roots, stems, leaves and flowers of A. biebersteinii in 100 ml of distilled water for 24 h and distilled water was used as the control...
June 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/27165526/salicylic-acid-induced-cysteine-protease-activity-during-programmed-cell-death-in-tomato-plants
#18
Judit Kovács, Péter Poór, Ágnes Szepesi, Irma Tari
The hypersensitive response (HR), a type of programmed cell death (PCD) during biotic stress is mediated by salicylic acid (SA). The aim of this work was to reveal the role of proteolysis and cysteine proteases in the execution of PCD in response of SA. Tomato plants were treated with sublethal (0.1 mM) and lethal (1 mM) SA concentrations through the root system. Treatment with 1 mM SA increased the electrolyte leakage and proteolytic activity and reduced the total protein content of roots after 6 h, while the proteolytic activity did not change in the leaves and in plants exposed to 0...
June 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/27165525/complete-mitochondrial-genome-sequence-of-a-hungarian-red-deer-cervus-elaphus-hippelaphus-from-high-throughput-sequencing-data-and-its-phylogenetic-position-within-the-family-cervidae
#19
Krisztián Frank, Endre Barta, Nóra Á Bana, János Nagy, Péter Horn, László Orosz, Viktor Stéger
Recently, there has been considerable interest in genetic differentiation in the Cervidae family. A common tool used to determine genetic variation in different species, breeds and populations is mitochondrial DNA analysis, which can be used to estimate phylogenetic relationships among animal taxa and for molecular phylogenetic evolution analysis. With the development of sequencing technology, more and more mitochondrial sequences have been made available in public databases, including whole mitochondrial DNA sequences...
June 2016: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/27165524/effects-of-high-ambient-temperature-on-fish-sperm-plasma-membrane-integrity-and-mitochondrial-activity-a-flow-cytometric-study
#20
Szabolcs Tamás Nagy, Balázs Kakasi, László Pál, Máté Havasi, Miklós Bercsényi, Ferenc Husvéth
Local extreme climatic conditions occurring as a result of global climate change may interfere with the reproduction of animals. In the present study fish spermatozoa were incubated at different temperatures (20, 25, 30 and 40 °C) for 10 and 30 minutes, respectively and plasma membrane integrity and mitochondrial membrane potential changes were evaluated with flow cytometry using SYBR-14/PI and Mitotracker Deep Red FM fluorescent dyes. No significant differences were found in plasma membrane integrity at either incubation temperatures or time points...
June 2016: Acta Biologica Hungarica
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