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General Physiology and Biophysics

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https://www.readbyqxmd.com/read/29589837/blood-pressure-management-and-progression-of-chronic-kidney-disease-in-a-canine-remnant-kidney-model
#1
Jun G Kang, Mi-Yeon Yu, Hajeong Lee, Dong K Kim, Kwon W Joo, Yon S Kim, Seung H Yang
The canine remnant kidney model is fundamental to understanding the relationship between hypertension and chronic kidney disease (CKD). This study aimed to create a 1/16 remnant kidney model and to determine whether blood pressure (BP) control affects the progression of CKD. A group of dogs received BP treatment (group A) and another received BP treatment except for the first 2 weeks (group B). The remnant kidney model was induced using a two-step subtotal nephrectomy method; dogs received antihypertensive therapy...
March 28, 2018: General Physiology and Biophysics
https://www.readbyqxmd.com/read/29589836/laser-induced-calcium-oscillations-in-fluorescent-calcium-imaging
#2
János Vincze, Nikolett Geyer, Gyula Diszházi, László Csernoch, Tamás Bíró, István Jóna, Beatrix Dienes, János Almássy
Phototoxicity is the most common problem investigators may encounter when performing live cell imaging. It develops due to excess laser exposure of cells loaded with fluorophores and can lead to often overlooked but significant artifacts, such as massive increase of intracellular Ca2+ concentration, which would make data interpretation problematic. Because information about laser- and dye-related changes in cytoplasmic calcium concentration is very limited, we aimed to describe this phenomenon to help investigators using laser scanning confocal microscopy in a non-invasive way...
March 28, 2018: General Physiology and Biophysics
https://www.readbyqxmd.com/read/29589835/snc80-and-naltrindole-modulate-voltage-dependent-sodium-potassium-and-calcium-channels-via-a-putatively-delta-opioid-receptor-independent-mechanism
#3
Lucia Moravčíková, Jana Královičová, Ľubica Lacinová
SNC80 was designed as a highly selective nonpeptide delta opioid receptor (DOR) agonist. Antidepressant-like and antinociceptive effects of this compound were demonstrated in animal models. Naltrindole was synthetized as a highly selective DOR antagonist. Its antitussive and antinociceptive effects were reported. Observed effects of SNC80 and naltrindole may be accompanied by changes in neuronal excitability including modulation of voltage-dependent ion channels. We investigated possible DOR-independent modulation of neuronal sodium, calcium and potassium currents by both agents...
March 28, 2018: General Physiology and Biophysics
https://www.readbyqxmd.com/read/29593129/novel-neuroprotective-role-of-hydrogen-sulfide-in-a-rat-model-of-stress-brain-injury
#4
Eman A Elbassuoni, Walaa H Nazmy
Hydrogen sulfide (H2S) is a gaseous mediator recognized as important neuromodulator agent in the central nervous system. Since stress is among the most important factors involved in several pathophysiological brain processes. This study aim to investigate the effect of exogenous H2S on the possible negative effect of stress on the brain of rats and the underlying mechanisms. Rats were divided into 3 groups: control, stressed, H2S treated + stress. Brain injury markers measured were serum S100 protein and gamma enolase...
March 2018: General Physiology and Biophysics
https://www.readbyqxmd.com/read/29593128/selective-inhibition-of-smooth-muscle-plasma-membrane-transport-%C3%B0-%C3%B0-2-mg2-%C3%B0-%C3%B0-%C3%B0-ase-by-calixarene-c-90-and-its-activation-by-ipt-35-compound
#5
Iuliia I Mazur, Tetyana O Veklich, Oleksandr A Shkrabak, Nikolai A Mohart, Anatoly M Demchenko, Inna V Gerashchenko, Roman V Rodik, Vitaly I Kalchenko, Sergiy O Kosterin
We investigated the influence of calixarene C-90 and IPT-35 on plasma membrane Ca2+- pumping АТРase (PMCA), intracellular calcium homeostasis and myometrium smooth muscle strain contractions. It has been shown that both effectors (100 μM) affect PMCA enzymatic activity: calixarene C-90 inhibits it by 75% and IPT-35 activates it by 40%. These compounds don't affect the Mg2+-АТРase, Mg2+-independent Са2+-АТРase and Na+,K+-АТРase enzymatic activities. C-90 inhibition coefficient I0.5 magnitude was approximately 20 μM and the Hill coefficient nH was 0...
March 2018: General Physiology and Biophysics
https://www.readbyqxmd.com/read/29593127/sulfamethoxazole-induces-zinc-changes-at-hippocampal-mossy-fiber-synapses-from-pregnant-rats
#6
Vanessa N Corceiro, Fatima C Bastos, Carlos M Matias, Jose C Dionísio, Rosa M Santos, Luis M Rosario, Rosa M Quinta-Ferreira, M Emilia C Quinta-Ferreira
The accumulation of intracellular ionic zinc and pharmaceutical compounds, like the antibiotic sulfamethoxazole, may contribute to various neuropathologies. Sulfamethoxazole and the drug trimethoprim, are inhibitors of enzymes involved in the synthesis of tetrahydrofolate and also of carbonic anhydrases. The inhibition of the latter enzymes, which are localized both intra- and extracellularly and have a key role in pH regulation, causes alkalinization that is associated with higher spontaneous transmitter release...
March 2018: General Physiology and Biophysics
https://www.readbyqxmd.com/read/29593126/effect-of-the-allopregnanolone-and-allotetrahydrodeoxycorticosteron-on-spike-wave-discharges-in-the-eeg-of-absence-epilepsy-rat-models
#7
Sebahattin Karabulut, Ruslan Bayramov, Keziban Korkmaz Bayramov, Ahmet K Filiz, Ahmet S Taskiran, Ercan Ozdemir
Absence epilepsy is a generalized nonconvulsive type of epilepsy that is characterized by spike-wave discharges (SWD) with a frequency of 2.5-4 Hz in the EEG. The activation of the GABAergic system in central nervous system suppresses convulsive seizures but exacerbates absence seizures. Endogenous neuroactive steroids such as 3α-hydroxy-5α-pregnan-20-one (3α,5α-THPROG; allopregnanolone) and 3α,21-dihydroxy-5α-pregnan-20-one (3α,5α-THDOC, allotetrahydrodeoxycorticosteron) are GABA-A receptor-positive allosteric modulators...
March 2018: General Physiology and Biophysics
https://www.readbyqxmd.com/read/29593125/upregulation-of-heme-oxygenase-1-ho-1-attenuates-kidney-damage-oxidative-stress-and-inflammatory-reaction-during-renal-ischemia-reperfusion-injury
#8
Mai Barakat, Mahmoud M Gabr, Faten Zhran, Mohamed El-Adl, Abdelaziz M Hussein, Nashwa Barakat, Reda Eldemerdash
The current study investigated the effect of upregulation of heme oxygenase 1 (HO-1) by cobalt protoporphyrin (CoPP) on renal dysfunctions in renal ischemia/reperfusion (I/R) injury and its underlying mechanisms. 72 male Sprague Dawley rats were divided into 4 groups: sham group, ischemic group (left 45-min renal ischemia), CoPP-before group (as ischemic group with CoPP 20 mg/kg 30 min before ischemia) and CoPP-after group (as ischemic group with CoPP 20 mg/kg 20 min after ischemia). Serum creatinine, urea and TGF-β1 and markers of redox state (MDA, SOD, GSH and CAT), nitric oxide (NO), TGF-β1 and HO-1 in kidney tissues were measured...
March 2018: General Physiology and Biophysics
https://www.readbyqxmd.com/read/29593124/association-between-3-utr-polymorphisms-in-genes-acvr2a-agtr1-and-rgs2-and-preeclampsia
#9
Andrea Mendelova, Veronika Holubekova, Marian Grendar, Pavol Zubor, Iveta Svecova, Dusan Loderer, Zuzana Snahnicanova, Kamil Biringer, Jan Danko, Zora Lasabova
Preeclampsia (PE) is a pregnancy specific disease with several risk factors such as genetic polymorphisms, environmental and social factors participating in its development. The aim of this study was to investigate whether distribution of three putative regulatory SNPs rs13430086, rs5186, rs4606 in 3'UTR of genes ACVR2A, AGTR1 and RGS2, respectively, that have been associated with hypertension and regulation of trophoblast invasion differ between women with PE and control group. The associations of rs13430086, rs5186 and rs4606 with preeclampsia were tested in two groups - the group of 50 women with PE and the control group of 42 healthy pregnant women at term...
March 2018: General Physiology and Biophysics
https://www.readbyqxmd.com/read/29593123/toxicogenomic-evaluation-of-liver-responses-induced-by-acrylamide-and-glycidamide-in-male-mouse-liver
#10
Dongyan Chen, Huangyou Liu, Enting Wang, Haiyang Yan, Haiqing Ye, Yuan Yuan
In current studies, histopathologic method, Agilent GeneChip hybridization and Western blot were used to investigate the toxicity of acrylamide (AA) and glycidamide (GA) in male mouse livers. The histopathologic results demonstrated that AA and GA could cause oxidative damage to mouse liver. Middle dose of GA and AA (50 mg/kg b.w./day) could significantly up-regulate the expression of cytochrome P450, as well as genes related to oxidative injury, cancer and inflammation, and significantly down-regulate the expression of genes related to anti-apoptosis, antioncogene and fatty acid synthesis...
March 2018: General Physiology and Biophysics
https://www.readbyqxmd.com/read/29593122/inhibition-of-at1-receptors-by-losartan-affects-myocardial-slow-force-response-in-healthy-but-not-in-monocrotaline-treated-young-rats
#11
Oleg Lookin, Alexandr Balakin, Yuri Protsenko
The slow force response (SFR) of a cardiac muscle to a sudden stretch is thought to be important in the regulatory adaptation of myocardial contraction. Autocrine-paracrine regulation pathways which involve angiotensin II are participating in this mechanism. On the other hand, renin-angiotensin-aldosterone system (RAS) is altered in hypertrophic or failing myocardium. We compared the effects of sudden stretch to SFR as well as to twitch and Ca2+ transient characteristics in rat myocardium with monocrotaline-induced heart failure with those in normal rat myocardium without and with inhibition of angiotensin II type-1 (AT1) receptors...
March 2018: General Physiology and Biophysics
https://www.readbyqxmd.com/read/29593121/proteomic-analysis-of-human-glutathione-transferase-omega-hgsto1-stable-transfection-in-a-6-hydroxydopamine-induced-neuronal-cells
#12
Jeerang Wongtrakul, Chonticha Saisawang, Benjawan Kumrapich, Jiraprapa Wipasa, Sittiruk Roytrakul, Albert J Ketterman
Parkinson's disease is the second most common neurodegenerative disorder after Alzheimer's disease. The disease is associated with dopaminergic neuron losses in the substantia nigra area of the brain and the formation of cytoplasmic inclusion bodies. Human glutathione transferase omega 1 (hGSTO1) appears to have a role in modulating stress response. The study was aimed to elucidate differentially expressed proteins caused by oxidative stress induced by 6-hydroxydopamine (6-OHDA). Human neuronal cells SH-SY5Y overexpressing hGSTO1 were used to investigate protein glutathionylation and the modulation of cellular protein expression...
March 2018: General Physiology and Biophysics
https://www.readbyqxmd.com/read/29593120/ros-mediated-activation-of-ampk-plays-a-critical-role-in-sulforaphane-induced-apoptosis-and-mitotic-arrest-in-ags-human-gastric-cancer-cells
#13
Yung H Choi
In this study, we investigated the role of AMP-activated protein kinase (AMPK) and the importance of reactive oxygen species (ROS) in apoptosis induction associated with cell cycle arrest induced by sulforaphane in AGS human gastric cancer cells. Our results demonstrated that sulforaphane inhibited proliferation of AGS cells by promoting apoptosis and accumulating the cellular portion of the G2/M phase via the buildup of cyclin B1 and cyclin-dependent kinase p21 (WAF1/CIP1). Moreover, the phosphorylation of histone H3 was markedly increased following treatment with sulforaphane, indicating that sulforaphane stimulated mitotic arrest...
March 2018: General Physiology and Biophysics
https://www.readbyqxmd.com/read/29593119/analysis-of-the-binding-mode-of-a-novel-hif1%C3%AE-inhibitor-through-molecular-modelling
#14
Yi Zhao, Ying Li, Ju H Wang, Jia Zhang, Xu Zhang
Hypoxia inducible factor-1 (HIF1) is an important transcription factor related with tumor metastasis. As a subunit of HIF1, HIF1α plays an important role in regulation of the hypoxic response. HIF1α inhibitor could be a promising treatment for certain cancers. In the present study, we try to model the binding mode of the recent reported new series of HIF1α inhibitors with the purpose of further improving the performance of these inhibitors. Molecular docking was first employed to predict the binding modes of the protein-ligand complexes, followed by molecular dynamics simulations and MMGBSA free energy calculations...
March 2018: General Physiology and Biophysics
https://www.readbyqxmd.com/read/29424356/water-soluble-quercetin-modulates-the-choleresis-and-bile-lipid-ratio-in-rats
#15
Tatiana Vovkun, Petro Yanchuk, Lidiya Shtanova, Stanislav Veselskiy, Natalia Filimonova, Anatoly Shalamay, Volodymyr Vedmid
Water-soluble analogue of quercetin, corvitin is used in patients with myocardial infarction as blocker of 5-lipoxygenase. However, its effects on secretion, lipid content and physico-chemical properties of bile have not been understood yet. We investigated the effect of corvitin, applied in different doses, on the level of bile flow, the content of bile free and esterified cholesterol, phospholipids, triacylglycerols, and free fatty acids. In order to determine stability of the bile colloidal system, we examined the relationship between different lipid components...
January 2018: General Physiology and Biophysics
https://www.readbyqxmd.com/read/29424355/cytotoxicity-of-propolis-nanopreparations-in-cancer-cell-monolayers-multimode-of-action-including-apoptotsis-and-nitric-oxide-production
#16
Mahmoud S Sherif, Taha A Rehab, Zayed A Hamdia, Vladmir P Torchilin
Natural products are invaluable resource of anticancer drug discovery. They generally viewed as safe but weak, within the framework of nanotechnology, they can serve as template for potent anticancer drugs. We first evaluated the cytotoxic activity of different propolis extracts (water, 70% ethanol, absolute ethanol and hexane) in many cancer cell lines, then the solid nanoparticles from the organic solvent extracts were prepared and their cytotoxicity was evaluated as well. Finally, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) liposomes were prepared from the most cytotoxic organic solvent extract and their cytotoxicity was also evaluated...
January 2018: General Physiology and Biophysics
https://www.readbyqxmd.com/read/29424354/stability-studies-of-endocrine-disrupting-tributyltin-and-triphenyltin-compounds-in-an-artificial-sea-water-model
#17
Ladislav Novotny, Leyla Sharaf, Mohammed E Abdel-Hamid, Julius Brtko
Triorganotins belong to toxic components present predominantly in antifouling paints for marine vessels. Tributyltin/triphenyltin at pico- or nanomolar concentrations in sea water are known to induce an irreversible sexual abnormality in females of over 190 marine species, an "imposex" phenomenon - the superimposition of male genitalia on a female. Moreover, trialkyltins and triaryltins function as potent nuclear retinoid X receptors (RXR) agonists. In mammals, triorganotin compounds induce immunosuppressive, metabolic, reproductive or developmental effects...
January 2018: General Physiology and Biophysics
https://www.readbyqxmd.com/read/29424353/resuscitative-therapy-with-erythropoietin-reduces-oxidative-stress-and-inflammatory-responses-of-vital-organs-in-a-rat-severe-fixed-volume-hemorrhagic-shock-model
#18
Mina Ranjbaran, Mehri Kadkhodaee, Behjat Seifi, Reza Mirzaei, Parisa Ahghari
Hemorrhagic shock (HS) still has a high mortality rate and none of the known resuscitative regimens completely reverse its adverse outcomes. This study investigated the effects of different models of resuscitative therapy on the healing of organ damage in a HS model. Male Wistar rats were randomized into six groups: Sham, without HS induction; HS, without resuscitation; HS+Blood, resuscitation with the shed blood; HS+Blood+NS, resuscitation with blood and normal saline; HS+Blood+RL, resuscitation with blood and Ringer's lactate; EPO, erythropoietin was added to the blood and RL...
January 2018: General Physiology and Biophysics
https://www.readbyqxmd.com/read/29424352/dna-dope-gemini-surfactants-complexes-at-low-surface-charge-density-from-structure-to-transfection-efficiency
#19
Lukáš Hubčík, Dominika Galliková, Petra Pullmannová, Ľubica Lacinová, Zdena Sulová, Mária Hanulová, Sergio S Funari, Ferdinand Devínsky, Daniela Uhríková
DNA condensation, structure and transfection efficiency of complexes formed by gemini surfactants alkane-α,ω-diyl-bis(dodecyldimethylammonium bromide)s (CnGS12, n = 3, 6 and 12 is the number of alkane spacer carbons), dioleoylphosphatidylethanolamine (CnGS12/DOPE = 0.3 mol/mol) and DNA at low surface charge density were investigated through different techniques. Small angle X-ray diffraction showed a condensed lamellar phase with marked dependence of DNA-DNA distance on (+/-) charge ratio. High ionic strength of hydrating medium screens the interaction DNA - CnGS12/DOPE and complexed DNA represented maximally ~ 45-60% of total DNA in the solution as derived from fluorescence and UV-VIS spectroscopy...
January 2018: General Physiology and Biophysics
https://www.readbyqxmd.com/read/29424351/ca2-dependent-calcineurin-nfat-signaling-in-%C3%AE-adrenergic-induced-cardiac-hypertrophy
#20
Ali Khalilimeybodi, Alireza Daneshmehr, Babak Sharif Kashani
Ca2+ is an important mediator in the β-adrenergic-induced cardiac hypertrophy. The β-adrenergic stimulation alters the Ca2+ transient characteristics including its oscillation frequency, diastolic and systolic levels which lead to the CaN activation and subsequent NFAT-dependent hypertrophic genes transcription. Moreover, β-adrenergic-induced alterations in PKA and GSK3β kinase activities in both the cytosol and the nucleus regulate NFAT nuclear translocation and contribute in its hypertrophic response...
January 2018: General Physiology and Biophysics
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