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Journal of Pharmaceutical and Biomedical Analysis

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https://www.readbyqxmd.com/read/30412806/in-vitroand-in-vivo-investigation-of-metabolic-fate-of-riociguat-by-hplc-q-tof-ms-ms-and-in-silico-evaluation-of-the-metabolites-by-admet-predictor-%C3%A2
#1
Shristy S Tiwari, Balasaheb B Chavan, Bhoopendra S Kushwah, Naga Veera Yerra, Sumit Mukesh, Abhay T Sangamwar, Jagadeshwar Reddy Thaota, M V N Kumar Talluri
Riociguat, a guanyl cyclase inhibitor, is one of its kind drug regimen approved for management of pulmonary arterial hypertension and chronic thromboembolism pulmonary hypertension. Extensive literature review indicates lack of comprehensive reports on its metabolic fate. The present study reports the in vivo and in vitro identification and characterization of metabolites of riociguat, using high-performance liquid chromatography-quadruple time-of-flight tandem mass spectrometry. In vitro studies were conducted by incubating the drug in human and rat liver microsomes in presence of respective cofactors...
November 1, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30412803/ultra-performance-liquid-chromatography-coupled-with-principal-component-and-cluster-analysis-of-swertia-chirayita-for-adulteration-check
#2
Manju Singh, Ram Mohan, Sonali Mishra, Nitu Goyal, Karuna Shanker, Namita Gupta, Birendra Kumar
This article describes the study to standardize phytochemically and distinguish Swertia chirayita from that of possible substitution/adulteration using ultra performance liquid chromatography (UPLC) with photodiode array detector (PDA) and chemometric tools viz. principal component analysis (PCA) and hierarchical clustering analysis (HCA). Five ecotypes of Swertia chirayita and five possible substitutions, e.g.,Swertia bimaculata (SB), Swertia chordata (SCH), Swertia ciliata (SCL), Swertia paniculata (SP), and Halenia elliptica (HE) collected from different Indian Himalayan region...
November 1, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30415142/pharmacokinetic-study-of-sirolimus-ophthalmic-formulations-by-consecutive-sampling-and-liquid-chromatography-tandem-mass-spectrometry
#3
Ling Wang, Zhan Tang, Ming Shi, Qiao Wang
Sirolimus is regarded as one of the most effective immunosuppressants receiving extensive attention over the years, for which the ocular application needs further development in clinical keratoplasty. In order to study the transcorneal absorption effect of ophthalmic administration, there was a need to study the pharmacokinetics of drugs in aqueous humor. In this work, a validated and reliable HPLC-ESI-MS/MS method was established to study the pharmacokinetics of sirolimus nanoformulations in rabbit aqueous humor...
October 31, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30412802/quantification-of-2-aminoisobutyric-acid-impurity-in-enzalutamide-bulk-drug-substance-using-hydrophilic-interaction-chromatography-with-fluorescence-detection
#4
Michal Douša
A rapid procedure for the determination of 2-aminoisobutyric acid in enzalutamide bulk drug substance based on hydrophilic interaction chromatography with fluorescence detection was developed. Fluorescence detection after postcolumn derivatization with o-phthaldialdehyde/2-mercaptoethanol was carried out at excitation and emission wavelength of 345 nm and 450 nm, respectively. The postcolumn reaction conditions such as reaction temperature, mobile phase and derivatization reagent flow rate and the reagents concentrations were studied and optimized due to steric hindrance of amino group of 2-aminoisobutyric acid...
October 31, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30396052/non-invasive-quantification-of-malondialdehyde-biomarker-in-human-exhaled-breath-condensate-using-self-assembled-organic-inorganic-nanohybrid-a-new-platform-for-early-diagnosis-of-lung-disease
#5
Mohsen Jafari, Elham Solhi, Solmaz Tagi, Mohammad Hasanzadeh, Vahid Jouyban-Gharamaleki, Abolghasem Jouyban, Nasrin Shadjou
A novel electrochemical sensor was developed towards recognition of malondialdehyde (MDA) with modifying the glassy carbon electrode (GCE) by self-assembled riboflavin-taurine (RFPT) as organic substrate and silver nanoparticle (Ag NPs) as inorganic nano-catalyst. Therefore, a novel biocompatible and anti-bacterial substrate were prepared for the construction of electrode. In proposed sensor, the RF-PT polymer film led to the emergence of electroactive and suitable substrate for the immobilization of Ag NPs...
October 29, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30408624/concept-of-sample-specific-correction-of-immunoassay-results-for-precise-and-accurate-igg-quantification-in-horse-plasma
#6
Beata Halassy, Tihana Kurtović, Maja Lang Balija, Marija Brgles, Monika Tunjić, Dora Sviben
The hyperimmune horse plasma (HHP), prepared through active immunisation of horses with an antigen of interest, is the most common starting material for antitoxin (animal antibody-based therapeutics) production. Precise IgG quantification in plasma is a prerequisite for accurate estimation of the purification process efficiency. Although immunoglobulins from HHP have been purified for over a century, there is still no in vitro method for precise and accurate determination of IgG content in HHP. For this reason, the purification process efficiency has been assessed by antibody activity measurements, mostly performed in vivo...
October 28, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30396053/simultaneous-lc-ms-ms-analysis-of-simvastatin-atorvastatin-rosuvastatin-and-their-active-metabolites-for-plasma-samples-of-obese-patients-underwent-gastric-bypass-surgery
#7
Asma El-Zailik, Lily K Cheung, Yang Wang, Vadim Sherman, Diana S-L Chow
Statins, HMG-CoA reductase inhibitors, are considered the first line treatment of hyperlipidemia to reduce the risk of atherosclerotic cardiovascular diseases. The prevalence of hyperlipidemia and the risk of atherosclerotic cardiovascular diseases are higher in obese patients. Published methods for the quantification of statins and their active metabolites did not test for matrix effect of or validate the method in hyperlipidemic plasma. A sensitive, specific, accurate, and reliable LC-MS/MS method for the simultaneous quantification of simvastatin (SMV), active metabolite of simvastatin acid (SMV-A), atorvastatin (ATV), active metabolites of 2-hydroxy atorvastatin (2-OH-ATV), 4-hydroxy atorvastatin (4-OH-ATV), and rosuvastatin (RSV) was developed and validated in plasma with low (52-103 mg/dl, <300 mg/dl) and high (352-403 mg/dl, >300 mg/dl) levels of triglyceride...
October 28, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30391812/nuclear-magnetic-resonance-based-metabolomics-approach-to-evaluate-preventive-and-therapeutic-effects-of-gastrodia-elata-blume-on-chronic-atrophic-gastritis
#8
Cheng Chen, Yong-Hong Fu, Ming-Hui Li, Ling-Yu Ruan, Han Xu, Jian-Feng Chen, Wen-Long Zhao, Hui-Hui Meng, Yue-Xiao Xing, Wei Hong, Jun-Song Wang
Chronic atrophic gastritis (CAG) is one of the most common digestive system diseases worldwide which defined by WHO as initial step of cancer. Gastrodia elata Blume (GEB) is a traditional herbal with multiple pharmacological activities which was widely used in Asian countries. This study aims to explore the preventive and therapeutical effects of Gastrodia elata Blume on auto-immune induced CAG in rats. Tissues of stomachs were collected and submitted to 1 H NMR-based metabolomics analysis and histopathological inspection...
October 28, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30390561/metabolic-profiling-of-copper-laden-hepatolenticular-degeneration-model-rats-and-the-interventional-effects-of-gandou-decoction-using-uplc-q-tof-ms
#9
Meimei Cheng, Huan Wu, Hongfei Wu, Xiaochuang Liu, An Zhou
The direct cause of hepatolenticular degeneration (HLD) is copper metabolic disorder caused by autosomal recessive inheritance. Gandou decoction (GDD), a classical traditional Chinese medicine formula, exhibits unambiguous therapeutic effect on HLD in China for decades. However, the mechanism of effect on HLD is not clear. With this purpose, the effects of copper content and histopathology inspection on the HLD rat model were investigated. Then, metabolomics study based on ultra-performance liquid chromatography quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MSE ) analysis and multivariate statistical analysis was adopted to further reveal the mechanism of action of GDD against HLD...
October 28, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30391810/development-of-near-infrared-spectroscopic-calibration-models-for-in-line-determination-of-low-drug-concentration-bulk-density-and-relative-specific-void-volume-within-a-feed-frame
#10
Carlos Ortega-Zúñiga, Carlos Pinzón-De la Rosa, Andrés D Román-Ospino, Alberto Serrano-Vargas, Rodolfo J Romañach, Rafael Méndez
This study describes the development of a near infrared (NIR) calibration model for real time determination of drug concentration, powder density, and porosity or relative specific void volume (RSVV) of 3.00%w/w acetaminophen blends within a feed frame. The NIR calibration model was developed from 1.50 to 4.50%w/w of acetaminophen, using a high variability of major excipients (from 12.92 to 81.95%w/w) which facilitates the prediction of powder density and RSVV based on near infrared calibration spectra. The model using second derivative as spectral preprocessing explained the changes related to acetaminophen concentration in the first latent variable...
October 27, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30390560/determination-of-tyrosine-kinase-inhibitor-afatinib-in-rat-plasma-using-lc-ms-ms-and-its-application-to-in-vivo-pharmacokinetic-studies-of-afatinib-liposomes
#11
Xiaoyan Lu, Sha Liu, Xiucheng Yang, Meishan Han, Kaoxiang Sun
A sensitive quantitative liquid chromatography-tandem mass spectrometry assay for afatinib in rat plasma was developed and validated using afatinib dimaleate as a standard. The analyte was determined to be ionized using the positive ion multiple reaction monitoring mode through electrospraying on an AB Sciex Triple Quad™ 4500 system. Protein precipitation was used for sample preparation, and an Agilent Eclipse XDB-CN column (100 × 2.1 mm, 3.5 μm) was applied for chromatographic separation. The mobile phase was water and methanol (15:85, v/v) containing 0...
October 26, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30412805/preliminary-pharmacokinetic-study-of-the-anticancer-6bio-in-mice-using-an-uhplc-ms-ms-approach
#12
Job Tchoumtchoua, Maria Halabalaki, Evangelos Gikas, Anthony Tsarbopoulos, Nikoletta Fotaki, Lucy Liu, Sangkil Nam, Richard Jove, Leandros A Skaltsounis
Indirubins represent a group of natural and synthetic products with bio-activities against numerous human cancer cell lines acting by inhibiting protein kinases. The natural sources of indirubins are plants of Isatis sp., Indigofera sp., and Polygonum sp., recombinant bacteria, mammalian urine and some marine mollusks. Specifically, the halogenated derivative 6-bromo indirubin-3'-oxime (6BIO) possesses increased selectivity against GSK-3. However, to our knowledge, no analytical method to determine 6BIO in biological fluids has been developed till now...
October 25, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30412804/succinic-acid-enhanced-quantitative-determination-of-blood-modified-nucleosides-in-the-development-of-diabetic-nephropathy-based-on-hydrophilic-interaction-liquid-chromatography-mass-spectrometry
#13
Mengzhe Guo, Dantong Liu, Qian Sha, Houfa Geng, Jun Liang, Daoquan Tang
The epigenetic modification of DNA or RNA may have relationship with the development of diabetic nephropathy (DN). We designed to develop a liquid chromatography mass spectrometry (LC-MS) coupled with mobile phase additive sensitization method for the analysis of the dissociative epigenetic modified nucleosides in serum of patients from different periods of DN with the health comparison. Serum samples were collected from 43 healthy volunteers and 156 patients, which divided into four groups. A succinic acid enhanced LC-MS/MS strategy was developed for the quantitative analysis of seven nucleosides in serum samples...
October 25, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30396051/rapid-identification-of-urokinase-plasminogen-activator-inhibitors-from-traditional-chinese-medicines-based-on-ultrafiltration-lc-ms-and-in-silico-docking
#14
Li Li, Jing Kong, Chun-Hua Yao, Xiu-Feng Liu, Ji-Hua Liu
The urokinase plasminogen activator (uPA) is regarded as the crucial trigger for plasmin generation, which is involved in several diseases especially for neoplasm metastasis. In this study, an efficient approach integrating ultrafiltration, LC/MS, bioassay and in silico docking, was proposed for rapidly detecting uPA ligands from Traditional Chinese Medicines (TCMs). Forty-two TCMs were initially assessed, and as illustrative case studies, Galla Chinensis and Sanguisorbae Radix, which appeared significant inhibitory activities on uPA, were chosen to develpe and verify the strategy...
October 25, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30390562/distribution-kinetics-of-puerarin-in-rat-hippocampus-after-acute-local-cerebral-ischemia
#15
Hui Kong, Guiliang Zhang, Jinjun Cheng, Rongfeng Shi, Meiling Zhang, Peng Cao, Yan Zhao, Huihua Qu, Qingguo Wang
Puerarin, isolated from the roots of Pueraria lobata, is widely used for treating cerebral ischemia in China. The time- and dose-dependent distribution characteristics of puerarin in the ischemic hippocampus are unknown. In this study, puerarin concentration was determined by an indirect competitive ELISA using anti-puerarin monoclonal antibody. Area under the curve (AUC0-120 min ) of puerarin (80 mg/kg) in the embolic hippocampus was higher than that in the normal hippocampus; the increase was significant only at 40 and 20 mg/kg...
October 25, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30390559/micellar-liquid-chromatographic-method-for-the-simultaneous-determination-of-citalopram-hydrobromide-with-its-two-demethylated-metabolites-utility-as-a-diagnostic-tool-in-forensic-toxicology
#16
D El Sherbiny, M E K Wahba
A micellar liquid chromatographic method has been developed for the simultaneous determination of citalopram hydrobromide (CTA) with its two demethylated metabolites namely; desmethyl citalopram hydrochloride (DCTA) and didesmethyl citalopram tartrate (DDCTA). Separation was conducted on a C18 column using a mobile phase composed of 0.18 M sodium dodecyl sulphate (SDS), 15% 1-propanol, 0.3% tri-ethylamine, adjusted to pH 4 with 0.2 M o-phosphoric acid and adopting UV detection at 240 nm. Analysis was performed at 60 °C applying a flow rate of 2 mL/min...
October 25, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30399533/analysis-of-bupivacaine-enantiomers-in-plasma-as-total-and-unbound-concentrations-using-lc-ms-ms-application-in-a-pharmacokinetic-study-of-a-parturient-with-placental-transfer
#17
Marília Cristina Oliveira Souza, Maria Paula Marques, Geraldo Duarte, Vera Lucia Lanchote
Bupivacaine, a drug used in obstetric anesthesia and analgesia, is commercially available as a racemic mixture of the R-bupivacaine and S-bupivacaine enantiomers, which show differences in pharmacokinetics, efficacy and toxicity. Changes in bupivacaine plasma protein binding is of clinical relevance considering its high protein binding (approximately 95%) and its classification as an intermediate hepatic extraction ratio drug (E = 0.38). Furthermore, the plasma protein binding of bupivacaine is also of clinical relevance considering that pregnancy is a physiological condition associated with reduced plasma albumin concentration...
October 23, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30412801/chemical-profiles-and-quality-evaluation-of-buddleja-officinalis-flowers-by-hplc-dad-and-hplc-q-tof-ms-ms
#18
Guoyong Xie, Qiuhong Xu, Ran Li, Lu Shi, Yu Han, Yan Zhu, Gang Wu, Minjian Qin
The dried flowers and inflorescences of Buddleja officinalis Maxim are used as traditional medicines in China, and aqueous extracts of the flowers have also been used since ancient times as a yellow rice colorant at local festivals. In this study, HPLC-Q-TOF-MS/MS was used to determine the overall chemical composition of this medicine-food plant. A total of 54 compounds, including 23 flavonoids, 19 phenylethanoid glycosides, 7 alkaloids and 5 other compounds, were detected in the methanol extracts of the herb using this method...
October 22, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30390557/determination-of-fucoxanthinol-in-rat-plasma-by-liquid-chromatography-tandem-mass-spectrometry
#19
Yiping Zhang, Jinjing Lin, Guangyu Yan, Wenhui Jin, Weizhu Chen, Jipeng Sun, Longhe Yang, Mingqing Huang, Zhuan Hong
Previous studies have indicated that dietary fucoxanthin is mainly converted into fucoxanthinol (the deacetylated form) in mammals, but the pharmacokinetics of fucoxanthinol remains unknown. In this study, after intravenous (i.v.) and intragastric gavage (i.g.) administration of fucoxanthinol to rats at 0.8 and 20 mg/kg respectively, one-step protein precipitation with methanol was employed to prepared plasma samples, and an accurate and precise liquid chromatography-tandem mass spectroscopy (LC-MS/MS) method was developed to determine fucoxanthinol...
October 22, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30390556/study-on-the-inhibitory-effect-of-furafylline-and-troleandomycin-in-the-7-methoxyresorufin-o-demethylase-and-nifedipine-oxidase-activities-in-hepatic-microsomes-from-four-poultry-species-using-high-performance-liquid-chromatography-coupled-with-fluorescence
#20
Hansen Murcia, Andrés Cruz, Jeffrey León, Miguel Ángel González-Curbelo
The present study reports the in vitro studies with furafylline and troleandomycin (TAO) as specific inhibitors of activities 7-methoxyresorufin-O-demethylase (MROD) and nifedipine oxidase, catalyzed by cytochrome P450 1 A2 (CYP1 A2) and 3A4 human enzymes, respectively, in hepatic microsomes of quail, duck, turkey and chicken. The results suggest that in chicken and quail the MROD activity is carried out by orthologs CYP1 A4 and 1 A5, meanwhile in duck and turkey by a CYP1 A5 ortholog. The nifedipine oxidase activity is carried out by orthologs of the CYP3A family in the four bird species...
October 22, 2018: Journal of Pharmaceutical and Biomedical Analysis
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