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Journal of Pharmaceutical and Biomedical Analysis

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https://www.readbyqxmd.com/read/30227355/process-analytical-technology-for-continuous-manufacturing-tableting-processing-a-case-study
#1
Victoria Pauli, Yves Roggo, Laurent Pellegatti, Nhat Quang Nguyen Trung, Frantz Elbaz, Simon Ensslin, Peter Kleinebudde, Markus Krumme
The use of Near Infrared Spectroscopy (NIRS) as a fast and non-destructive technique was employed for the control and monitoring of the tableting step during a continuous manufacturing process. Two NIRS methods were optimized in order to in-line control the blend uniformity in the tablet feed frame and the API concentration of freshly pressed tablets prior the ejection. The novelty of this work first lies in the acquisition speed of NIR spectra reaching up to 70,000 tablets/h. Partial Least Square (PLS) regression was used as chemometric tool for the computation that resulted in excellent predictive calibration results...
September 13, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30227356/urinary-and-plasma-metabolite-differences-detected-by-hplc-esi-qtof-ms-in-systemic-sclerosis-patients
#2
Álvaro Fernández-Ochoa, Rosa Quirantes-Piné, Isabel Borrás-Linares, David Gemperline, Marta E Alarcón Riquelme, Lorenzo Beretta, Antonio Segura-Carretero
Systemic Sclerosis (SSc) is a chronic autoimmune disease whose origin and pathogenesis are not yet well known. Recent studies are allowing a better definition of the disease. However, few studies have been performed based on metabolomics. In this way, this study aims to find altered metabolites in SSc patients in order to improve their diagnosis, prognosis and treatment. For that, 59 SSc patients and 28 healthy volunteers participated in this study. Urine and plasma samples were analysed by a fingerprinting metabolomic approach based on HPLC-ESI-QTOF-MS...
September 11, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30227357/an-icp-ms-platform-for-metal-content-assessment-of-cell-culture-media-and-evaluation-of-spikes-in-metal-concentration-on-the-quality-of-an-igg3-%C3%AE%C2%BA-monoclonal-antibody-during-production
#3
Adil Mohammad, Cyrus Agarabi, Sarah Rogstad, Elizabeth DiCioccio, Kurt Brorson, Muhammad Ashraf, Patrick J Faustino, Chikkathur N Madhavarao
Metal ions can be enzyme cofactors and can directly influence the kinetics of biochemical reactions that also influence the biological production and quality attributes of therapeutic proteins, such as glycan formation and distribution. However, the concentrations of metals in commercially available chemically defined media can range from 1 to 25,000 ppb. Because such concentration changes can impact cell growth, manufacturing yield and product quality the alteration/fluctuation in media composition should be well controlled to maintain product quality...
September 10, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30219598/diffusion-ordered-nmr-spectroscopy-measurements-as-screening-method-of-potential-reactions-of-api-and-excipients-in-drug-formulations
#4
Alexander Becht, Curd Schollmayer, Johannes Wiest, Detlef Heller, Wolfgang Baumann, Helmut Buschmann, Ulrike Holzgrabe
In the development of new pharmaceutical formulations it is important to consider the possible interactions between the active pharmaceutical ingredient (API) and excipients which is a well-known problem. The objective of the work presented here was to investigate such reactions by means of diffusion ordered NMR spectroscopy (DOSY). The known reaction of 5-aminosalicylic acid (5-ASA) and the excipient citric acid was studied. Three reaction products have been verified by DOSY, 1 H NMR and HPLC measurements...
September 8, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30219596/a-whole-blood-sample-to-answer-polymer-lab-on-a-chip-with-superhydrophilic-surface-toward-point-of-care-technology
#5
Kang Kug Lee, Myoung-Ok Kim, Seokheun Choi
An innovative sample-to-answer (S-to-A) polymer lab-on-a-chip (LOC) with a blood plasma separator based on asymmetric capillary force has been proposed, developed, and completely characterized for point-of-care technology (POCT) applications. A spray layer-by-layer (LbL) nanoassembly coating has been applied for the superhydrophilic surface onto the cyclic olefin copolymer (COC). Then, the developed superwetting surfaces were designed and optimized for three device applications such as lateral transportation of whole blood in the device by capillary pumping, on-chip whole blood/plasma separation with an asymmetric capillary force, and detection using a capillary-driven lateral flow colorimetric assay...
September 8, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30219599/development-of-formaldehyde-dehydrogenase-coupled-assay-and-antibody-based-assays-for-alkbh5-activity-evaluation
#6
Dan-Dan Shen, Feng-Zhi Suo, Qi-Meng Song, Jiao Chang, Ting Zhang, Jing-Jing Hong, Yi-Chao Zheng, Hong-Min Liu
N6-methyladenosine (m6 A) is the most prevalent internal modification of eukaryotic messenger RNA (mRNA). Until now, two RNA demethylases have been identified, including FTO (fat mass and obesity-associated protein) and ALKBH5 (α-ketoglutarate-dependent dioxygenase alkB homologue 5). As a mammalian m6 A demethylase, ALKBH5 significantly affects mRNA export and RNA metabolism as well as the assembly of mRNA processing factors in nuclear speckles, and ALKBH5 may play a significant role in these biological processes...
September 7, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30219597/an-lc-ms-ms-analysis-for-seven-sex-hormones-in-serum
#7
Teng-Fei Yuan, Juan Le, Yan Cui, Rui Peng, Shao-Ting Wang, Yan Li
BACKGROUND: Comprehensive serum sex hormone profiling is essential for monitoring the occurrence and development of many related diseases. However, the current methods for multi-class sex hormone detection were always lack of Standard Reference Material (SRM) certification and suffered from large sample consumption. For improvement, we developed a new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method focused on SRM certification and minimization of serum consumption for simultaneous quantification of seven mainstream serum sex hormones including estrogens (estrone E1, estradiol E2 and estriol E3), androgens (testosterone T, androstenedione AD, dehydroepiandrosterone DHEA) and progestogens (progesterone P)...
September 6, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30219595/from-1-h-nmr-based-non-targeted-to-lc-ms-based-targeted-metabolomics-strategy-for-in-depth-chemome-comparisons-among-four-cistanche-species
#8
Wenjing Liu, Qingqing Song, Yan Cao, Ning Xie, Zhiyong Li, Yong Jiang, Jian Zheng, Pengfei Tu, Yuelin Song, Jun Li
The great orthogonality between 1 H NMR spectroscopy and LC-MS implies that their deployments in series could offer an opportunity to gain the qualified molecular markers via comparative metabolomics, and an attempt was made here to propose an integrated strategy namely "from 1 H NMR-based non-targeted to LC-MS-based targeted metabolomics". In-depth chemome comparisons of Cistanche plants, such as C. deserticola, C. salsa, C. tubulosa, and C. sinensis, that possess dramatic economic and ecological benefits for the arid regions in the northwest China attributing to their dramatic medicinal and edible values, were employed to verify the applicability...
September 6, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30216793/quantification-of-isoflavone-glycosides-and-aglycones-in-rat-plasma-by-lc-ms-ms-troubleshooting-of-interference-from-food-and-its-application-to-pharmacokinetic-study-of-semen-sojae-praeparatum-extract
#9
Yuan Yao, Xin Ma, Teng Li, Hui Guo, Ruirui Chang, Jialin Liu, Qiaoxia Liu, Hongyuan Hao, Taohong Huang, Weidong Chen, Jun Wen, Tingting Zhou
The isoflavones widely exist in the daily diets and interferences are usually inevitable in the determination of the in vivo level of the same analytes. A new strategy to eliminate the dietary interference was established to evaluate the exposure of isoflavones including daidzin, glycitin, genistin, daidzein, glycitein, and genistein in rats fed with Semen Sojae Praeparatum (SSP) extract. Plasma samples were pretreated by liquid-liquid extraction with ethyl acetate using quercetin as the internal standard (IS)...
September 6, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30223144/use-of-ultra-high-performance-liquid-chromatography-coupled-with-quadrupole-time-of-flight-mass-spectrometry-system-as-valuable-tool-for-an-untargeted-metabolomic-profiling-of-rumex-tunetanus-flowers-and-stems-and-contribution-to-the-antioxidant-activity
#10
Jouda Abidi, Sonda Ammar, Samia Ben Brahim, Krystyna Skalicka-Woźniak, Zeineb Ghrabi-Gammar, Mohamed Bouaziz
Rumex tunetanus is an untapped resource with no available information about their chemical composition. That is why, the present study has conducted comprehensive metabolites profiling of the hydromethanol extracts of the Rumex tunetanus flowers and stems using RP-UHPLC-ESI-QTOF-MS. As results, 60 compounds were characterized for the first time in Rumex tunetanus between them, 18 photochemical were firstly identified in the Polygonaceae family in negative ionization mode. In this regard, quercetin-3-O-glucuronide and (-)-epicatechin gallate were the most abundant phenolic compounds in flowers and stems, respectively...
September 5, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30218948/distribution-patterns-for-metabolites-in-medicinal-parts-of-wild-and-cultivated-licorice
#11
Chengcheng Wang, Hao Cai, Hui Zhao, Ying Yan, Jingjing Shi, Shuyu Chen, Mengxia Tan, Jiali Chen, Lisi Zou, Cuihua Chen, Zixiu Liu, Chaoqie Xu, Xunhong Liu
Licorice is a famous Chinese medicinal material widely applied worldwide in food and drugs. It possesses a substantial share in the international and domestic markets. While the demand is continuously increasing, wild-type of licorice is gradually disappearing. Its cultivated-type is significantly different compared to its wild-type, especially the bioactive compounds in dried roots and rhizomes (the medicinal parts of wild and cultivated licorice) are responsible for their quality difference. In this study, a total of thirty-two constituents, including seventeen secondary compounds and fifteen primary metabolites, were simultaneously analyzed by UFLC coupled with triple quadrupole-linear ion trap mass spectrometry (UFLC-QTRAP-MS/MS) in different medicinal parts of licorice...
September 5, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30218717/hydrochlorothiazide-binding-to-human-serum-albumin-induces-some-compactness-in-the-molecular-structure-of-the-protein-a-multi-spectroscopic-and-computational-study
#12
Fatemeh Balaei, Sirous Ghobadi
The interaction between hydrochlorothiazide (HCTZ), a diuretic drug, with human serum albumin (HSA) was investigated by different biophysical approaches such as UV absorption, circular dichroism (CD), Fourier transform infrared (FT-IR), and fluorescence spectroscopy in 50 mM sodium phosphate buffer, pH 7.4. The results of fluorescence titration experiments revealed that HCTZ strongly quenches the intrinsic fluorescence of HSA through a static quenching mechanism. Binding constants and the number of binding sites were calculated using Stern-Volmer plots...
September 5, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30216794/determination-of-xanthones-and-flavonoids-of-methanol-extracts-obtained-from-different-parts-of-the-plants-of-three-gentianaceae-species
#13
Cheng-Yong Feng, Qian Wu, Dan-Dan Yin, Bing Li, Shan-Shan Li, Zhong-Qiu Tang, Yan-Jun Xu, Liang-Sheng Wang
Gentianopsis barbata, Halenia corniculata, and Gentianella acuta were widely distributed throughout China and commonly used in traditional Chinese medicine. However, owing to similar living environments and morphological features, locals often had trouble distinguishing between these three species. In this present study, chromatograms at 350 nm were obtained and the composition and content of their chemical compounds determined using HPLC-DAD/ESI-MS2 . In total, 35 chemical compounds were detected, 32 of which were identified, 25 of which were xanthones, 6 flavonoids, and 1 chlorogenic acid...
September 5, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30216792/comprehensive-quality-assessment-for-rhizoma-coptidis-based-on-quantitative-and-qualitative-metabolic-profiles-using-high-performance-liquid-chromatography-fourier-transform-near-infrared-and-fourier-transform-mid-infrared-combined-with-multivariate-statistical
#14
Luming Qi, Yuntong Ma, Furong Zhong, Chan Shen
Rhizoma Coptidis (RC) originated from the dried rhizomes of Coptis herbal species is a widely used traditional Chinese medicine in history. In this study, a comprehensive quality assessment for RC medicines from C. chinensis, C. deltoidea, C. omeiensis and C. teeta species was performed based on quantitative and qualitative metabolic profiles obtained from high performance liquid chromatography (HPLC), Fourier transform near-infrared (FT-NIR) and Fourier transform mid-infrared (FT-MIR) combined with multivariate statistical analysis...
September 5, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30205302/untargeted-search-and-identification-of-metabolites-of-antiviral-agent-camphecene-in-rat-urine-by-liquid-chromatography-and-mass-spectrometry-and-studying-their-distribution-in-organs-following-peroral-administration-of-the-compound
#15
Artem D Rogachev, Olga I Yarovaya, Alina V Fatianova, Valentina A Lavrinenko, Evgeny V Amosov, Vladimir V Zarubaev, Andrey G Pokrovsky, Nariman F Salakhutdinov
Major metabolites of camphecene, a new effective antiviral agent, formed after its oral administration to rats and excreted in the urine, were found and identified using liquid chromatography coupled to mass spectrometry as well as multivariate analysis of HPLC-MS data. The metabolites were found to be camphecene glucuronide, camphecene sulfate and the corresponding iminoacid. A study of the dynamics of accumulation of camphecene and its metabolites in the liver, kidneys, lungs and brain of animals was performed...
September 5, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30216791/chemical-biological-and-molecular-modelling-analyses-to-probe-into-the-pharmacological-potential-of-antidesma-madagascariense-lam-a-multifunctional-agent-for-developing-novel-therapeutic-formulations
#16
Mohamad Fawzi Mahomoodally, Arzu Atalay, Marie Carene Nancy Picot, Onur Bender, Evrim Celebi, Adriano Mollica, Gokhan Zengin
Antidesma madagascariense Lam. (AM), an indigenous medicinal plant to the Mascarene Islands, is used for the treatment of several diseases. We endeavoured to validate its use via evaluating the kinetics of inhibition of crude aqueous extract (CAE) and crude methanol extract (CME) of AM against key metabolic enzymes (pancreatic lipase, cholesterol esterase [CEase], acetylcholinesterase [AChE], and urease). In vitro antiglycation, antioxidant, cytotoxicity using iCELLigence real time cell analysis system and WST-1 methods, were used...
September 4, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30223142/sfc-for-chiral-separations-in-bioanalysis
#17
REVIEW
Lukas C Harps, Jan F Joseph, Maria K Parr
This review covers literature investigating methods for enantioselective chromatography using supercritical fluids as mobile phase constituents (SFC) in the field of bioanalysis. It provides an overview on method development and screening approaches published in scientific literature 2014-2018. Chiral stationary phases are used to create a chiral environment that allows for discrimination of enantiomers. Especially polysaccharide-based stationary phases are used in methods of recent investigations. In comparison to HPLC chiral SFC separation provides more selective cavity effects of inclusion-type chiral separation phases...
September 3, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30216789/fast-simultaneous-determination-of-main-components-and-impurity-sodium-ion-in-pama-injection-by-mixed-mode-chromatography
#18
Panpan Li, Wei Sun, Limin Zuo, Zhiling Zhu, Ting Zhao, Ran Wang, Minghui Zhu, Xiaoyun Leng, Xiaodan Qiu, Liping Bai, Guangzhi Shan
This study describes an analytical method to control the quality of potassium aspartate and magnesium aspartate (PAMA) injection based on the simultaneous detection of the main components (K+ , Mg2+ and Asp) and impurities (Na+ ) using a mixed-mode chromatography coupled with charged aerosol detector. To obtain optimal chromatographic separation, the effects of organic content, column temperature, buffer types, pH and concentrations were evaluated. A Response Surface Methodology (RSM) optimal design was performed after single factor experiment...
September 3, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30199809/investigation-of-the-inhibition-effect-of-arachidonic-acid-on-the-core-structure-of-the-hiv-1-gp41
#19
Xiaoying Xu, Lin Li, Zhen Liu, Xiaojun Yao, Xuanxuan Zhang, Shuwen Liu, Lihong Liu
The gp41 transmembrane domain of the envelope glycoprotein of the human immunodeficiency virus (HIV) modulates the conformation of the viral envelope spike. During the HIV fusion process, C-terminal heptad repeat (CHR, C34) wrap antiparallel to the N-terminal heptad repeat (NHR, N36) helices to form a stable six-helix bundle (6-HB) core structure, which brings the viral and cell membranes into close proximity for fusion. Therefore, inhibiting the formation of 6-HB is considered to be a key activity of an effective HIV-1 fusion inhibitor...
September 3, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/30205301/recent-advances-in-designing-nanomaterial-based-biointerfaces-for-electrochemical-biosensing-cardiovascular-biomarkers
#20
REVIEW
Leila Farzin, Mojtaba Shamsipur, Leila Samandari, Shahab Sheibani
Early diagnosis of cardiovascular disease (CVD) is critically important for successful treatment and recovery of patients. At present, detection of CVD at early stages of its progression becomes a major issue for world health. The nanoscale electrochemical biosensors exhibit diverse outstanding properties, rendering them extremely suitable for the determination of CVD biomarkers at very low concentrations in biological fluids. The unique advantages offered by electrochemical biosensors in terms of sensitivity and stability imparted by nanostructuring the electrode surface together with high affinity and selectivity of bioreceptors have led to the development of new electrochemical biosensing strategies that have introduced as interesting alternatives to conventional methodologies for clinical diagnostics of CVD...
September 1, 2018: Journal of Pharmaceutical and Biomedical Analysis
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