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Journal of Experimental & Clinical Cancer Research: CR

Jian Wu, Sheng Chen, Hao Liu, Zhe Zhang, Zhen Ni, Jie Chen, Zhiping Yang, Yongzhan Nie, Daiming Fan
BACKGROUND: Multidrug resistance remains a major obstacle to successful treatment for patients with gastric cancer (GC). Recently, glycosylation has been demonstrated to play a vital role in the acquisition of multidrug resistance. As a potent inhibitor of glycosylation, tunicamycin (Tu) has shown marked antitumor activities in various cancers. In the present study, we attempted to determine the exact effect of Tu on the chemoresistance of GC. METHODS: The cytotoxic effects of drugs on GC cells were evaluated by cell viability assays, and apoptosis was detected by flow cytometry...
November 9, 2018: Journal of Experimental & Clinical Cancer Research: CR
Wuhua Zhou, Li Gong, Qinchuan Wu, Chunyang Xing, Bajin Wei, Tianchi Chen, Yuan Zhou, Shengyong Yin, Bin Jiang, Haiyang Xie, Lin Zhou, Shusen Zheng
In the publication of this article [1], there are two inadvertent errors.
November 7, 2018: Journal of Experimental & Clinical Cancer Research: CR
Zoltán Hujber, Gergő Horváth, Gábor Petővári, Ildikó Krencz, Titanilla Dankó, Katalin Mészáros, Hajnalka Rajnai, Norbert Szoboszlai, William P J Leenders, András Jeney, László Tretter, Anna Sebestyén
BACKGROUND: Bioenergetic characterisation of malignant tissues revealed that different tumour cells can catabolise multiple substrates as salvage pathways, in response to metabolic stress. Altered metabolism in gliomas has received a lot of attention, especially in relation to IDH mutations, and the associated oncometabolite D-2-hydroxyglutarate (2-HG) that impact on metabolism, epigenetics and redox status. Astrocytomas and oligodendrogliomas, collectively called diffuse gliomas, are derived from astrocytes and oligodendrocytes that are in metabolic symbiosis with neurons; astrocytes can catabolise neuron-derived glutamate and gamma-aminobutyric acid (GABA) for supporting and regulating neuronal functions...
November 7, 2018: Journal of Experimental & Clinical Cancer Research: CR
Ke-Ting Que, Yun Zhou, Yu You, Zhen Zhang, Xiao-Ping Zhao, Jian-Ping Gong, Zuo-Jin Liu
BACKGROUND: MicroRNAs (miRNAs) posttranscriptionally regulate gene expression and thereby contribute to the modulation of numerous complex and disease-relevant cellular processes, including cell proliferation, cell motility, apoptosis and stress response. miRNA-31-5p is encoded on a genomic fragile site, 9p21.3, which is reportedly lost in many hepatocellular carcinoma (HCC) tumors. Based on previous findings, we hypothesized that miR-31-5p alters chemosensitivity and that miR-31-5p mimics may influence sensitivity to chemotherapeutics in HCC as well as in a variety of other cancers...
November 6, 2018: Journal of Experimental & Clinical Cancer Research: CR
Nian Liu, Kuan Song Wang, Min Qi, Ying Jun Zhou, Guang Yao Zeng, Juan Tao, Jian Da Zhou, Jiang Lin Zhang, Xiang Chen, Cong Peng
BACKGROUND: Vitex negundo L (Verbenaceae) is an aromatic shrub that is abundant in Asian countries. A series of compounds from Vitex negundo have been used in traditional Chinese medicine for the treatment of various diseases. Cutaneous melanoma is one of the most aggressive malignancies. A significant feature of melanoma is its resistance to traditional chemotherapy and radiotherapy; therefore, there is an urgent need to develop novel treatments for melanoma. METHODS: We first examined the effects of VB1 (vitexin compound 1) on cell viability by CCK-8 (cell counting kit) and Colony Formation Assay; And then, we analyzed the apoptosis and cell cycle by flow cytometry, verified apoptosis by Immunoblotting...
November 6, 2018: Journal of Experimental & Clinical Cancer Research: CR
Zeribe C Nwosu, Nadia Battello, Melanie Rothley, Weronika Piorońska, Barbara Sitek, Matthias P Ebert, Ute Hofmann, Jonathan Sleeman, Stefan Wölfl, Christoph Meyer, Dominik A Megger, Steven Dooley
In the publication of this article (1), there is an error in Fig. 5b. This has now been updated in the original article (1).
November 2, 2018: Journal of Experimental & Clinical Cancer Research: CR
Haotian Yang, Rehan M Villani, Haolu Wang, Matthew J Simpson, Michael S Roberts, Min Tang, Xiaowen Liang
Most chemotherapeutics elevate intracellular levels of reactive oxygen species (ROS), and many can alter redox-homeostasis of cancer cells. It is widely accepted that the anticancer effect of these chemotherapeutics is due to the induction of oxidative stress and ROS-mediated cell injury in cancer. However, various new therapeutic approaches targeting intracellular ROS levels have yielded mixed results. Since it is impossible to quantitatively detect dynamic ROS levels in tumors during and after chemotherapy in clinical settings, it is of increasing interest to apply mathematical modeling techniques to predict ROS levels for understanding complex tumor biology during chemotherapy...
November 1, 2018: Journal of Experimental & Clinical Cancer Research: CR
Qi Li, Chengya Dong, Jiayue Cui, Yubo Wang, Xinyu Hong
BACKGROUND: Glioblastoma multiforme (GBM) is the common primary brain tumor classified the most malignant glioma. Long non-coding RNAs (LncRNAs) are important epigenetic regulators with critical roles in cancer initiation and progression. LncRNA HOTAIRM1 transcribes from the antisense strand of HOXA gene cluster which locus in chromosome 7p15.2. Recent studies have shown that HOTAIRM1 is involved in acute myeloid leukemia and colorectal cancer. Here we sought to investigate the role of HOTAIRM1 in GBM and explore its mechanisms of action...
October 30, 2018: Journal of Experimental & Clinical Cancer Research: CR
Miyong Yun, Ae Jin Choi, Young Chan Lee, Munkyoo Kong, Ji-Youn Sung, Sung Soo Kim, Young-Gyu Eun
BACKGROUND: Human carbonyl reductase 1 (CBR1) plays major roles in protecting cells against cellular damage resulting from oxidative stress. Although CBR1-mediated detoxification of oxidative materials increased by stressful conditions including hypoxia, neuronal degenerative disorders, and other circumstances generating reactive oxide is well documented, the role of CBR1 under ionising radiation (IR) is still unclear. METHODS: The formalin-fixed and paraffin-embedded tissues of 85 patients with head and neck squamous cell carcinoma (HNSCC) were used to determine if CBR1 expression effects on survival of patients with treatment of radiotherapy...
October 30, 2018: Journal of Experimental & Clinical Cancer Research: CR
Cong Chen, Chao Ge, Zheng Liu, Liangyu Li, Fangyu Zhao, Hua Tian, Taoyang Chen, Hong Li, Ming Yao, Jinjun Li
BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most common malignant cancers with a high incidence and high mortality in East Asia. Identifying biomarkers and clarifying the regulatory mechanisms of HCC are of great importance. Herein, we report the role and mechanism of activating transcription factor 3 (ATF3), a member of the ATF/cAMP-responsive element-binding protein family of transcription factors in HCC. METHODS: ATF3 overexpression vector and shRNAs were transfected into HCC cancer cells to upregulate or downregulate ATF3 expression...
October 30, 2018: Journal of Experimental & Clinical Cancer Research: CR
Anand Rotte, Gabriella D'Orazi, Madhuri Bhandaru
This commentary wishes to highlight the 2018 Nobel Prize in Medicine awarded to two cancer immunotherapy scientists, Prof James Allison and Prof Tasuku Honjo, for their discovery on unleashing the body's immune system to attack cancer. Their studies have led to the development of an entire class of drugs that hopefully will bring lasting remissions to many patients who had run out of options.
October 30, 2018: Journal of Experimental & Clinical Cancer Research: CR
Yong Li, Fengjun Cao, Mingxing Li, Pindong Li, Yuandong Yu, Longchao Xiang, Tao Xu, Jinhua Lei, Yun Yan Tai, Jianyong Zhu, Bingbing Yang, Yingpin Jiang, Xiufang Zhang, Long Duo, Ping Chen, Xiongjie Yu
BACKGROUND: Lysosome-associated agents have been implicated as possible chemo-sensitizers and immune regulators for cancer chemotherapy. We investigated the potential roles and mechanisms of hydroxychloroquine (HCQ) in combination with chemotherapy in lung cancer treatment. METHODS: The effects of combined treatment on non-small cell lung cancer (NSCLC) were investigated using cell viability assays and animal models. The influence of HCQ on lysosomal pH was evaluated by lysosomal sensors and confocal microscopy...
October 29, 2018: Journal of Experimental & Clinical Cancer Research: CR
Shengming Deng, Guoxi Zhang, Jiajie Kuai, Peng Fan, Xuexiang Wang, Pei Zhou, Dan Yang, Xichen Zheng, Xiaomei Liu, Qunli Wu, Yuhui Huang
BACKGROUND: Antiangiogenic agents are commonly used in lung and colon cancer treatments, however, rapid development of drug resistance limits their efficacy. METHODS: Lentinan (LNT) is a biologically active compound extracted from Lentinus edodes. The effects of LNT on tumor angiogenesis were evaluated by immunohistochemistry in murine LAP0297 lung and CT26 colorectal tumor models. The impacts of LNT on immune cells and gene expression in tumor tissues were determined by flow cytometry, qPCR, and ELISA...
October 29, 2018: Journal of Experimental & Clinical Cancer Research: CR
Lu Xu, Yang Zhang, Kang Tian, Xi Chen, Rongxin Zhang, Xindi Mu, Yueguang Wu, Duchuang Wang, Shanshan Wang, Fang Liu, Taishu Wang, Jinrui Zhang, Shuyan Liu, Yingqiu Zhang, Caixia Tu, Han Liu
BACKGROUND: The PD-L1/PD-1 pathway blockade-mediated immune therapy has shown promising efficacy in the treatment of multiple cancers including melanoma. The present study investigated the effects of the flavonoid apigenin on the PD-L1 expression and the tumorigenesis of melanoma. METHODS: The influence of flavonoids on melanoma cell growth and apoptosis was investigated using cell proliferation and flow cytometric analyses. The differential IFN-γ-induced PD-L1 expression and STAT1 activation were examined in curcumin and apigenin-treated melanoma cells using immunoblotting or immunofluorescence assays...
October 29, 2018: Journal of Experimental & Clinical Cancer Research: CR
Yuting Gu, Fan Lv, Mingxing Xue, Kai Chen, Cheng Cheng, Xinyuan Ding, Min Jin, Guofeng Xu, Yanyun Zhang, Zhixiang Wu, Leizhen Zheng, Yeming Wu
BACKGROUND: Neuroblastoma (NB) is the most common pediatric solid tumor that originates from neural crest-derived sympathoadrenal precursor cells that are committed to development of sympathetic nervous system. The well differentiated histological phenotype of NB tumor cells has been reportedly associated with favorable patient outcome. Retinoic acid (RA) can effectively induce NB cell differentiation, thereby being used in the clinic as a treatment agent for inducing the differentiation of high-risk NB...
October 25, 2018: Journal of Experimental & Clinical Cancer Research: CR
Cornelia Braicu, Lajos Raduly, Gabriela Morar-Bolba, Roxana Cojocneanu, Ancuta Jurj, Laura-Ancuta Pop, Valentina Pileczki, Cristina Ciocan, Alin Moldovan, Alexandru Irimie, Alexandru Eniu, Patriciu Achimas-Cadariu, Angelo Paradiso, Ioana Berindan-Neagoe
BACKGROUND: Breast cancer is a highly heterogeneous pathology, exhibiting a number of subtypes commonly associated with a poor outcome. Due to their high stability, microRNAs are often regarded as non-invasive cancer biomarkers, having an expression pattern specific for their 'cell of origin'. METHOD: Triple negative breast cancer (TNBC: ER-, PR-, Her-2-) and double positive breast cancer (DPBC: ER+, PR+, Her-2) miRNA expression patterns were obtained by analysis of the TCGA (The Cancer Genome Atlas) data, followed by PCR-array analysis on plasma samples from 20 TNBC patients, 14 DPBC patients and 11 controls...
October 20, 2018: Journal of Experimental & Clinical Cancer Research: CR
Yuanyuan Yan, Fangxiao Liu, Li Han, Lin Zhao, Jianjun Chen, Olufunmilayo I Olopade, Miao He, Minjie Wei
BACKGROUND: Hypoxic tumor microenvironment and maintenance of stemness contribute to drug resistance in breast cancer. However, whether Hypoxia-inducible factor-2α (HIF-2α) in hypoxic tumor microenvironment mediates conversion to a stem cell phenotype and chemoresistance of breast tumors has not been elucidated. METHODS: The mRNA and protein expressions of HIF-1α, HIF-2α, Wnt and Notch pathway were determined using qRT-PCR and western blot. Cell viability and renew ability were assessed by MTT, Flow cytometric analysis and soft agar colony formation...
October 19, 2018: Journal of Experimental & Clinical Cancer Research: CR
Xiudong Guan, Lanxin Luo, Gulnaz Begum, Gary Kohanbash, Qingkun Song, Aparna Rao, Nduka Amankulor, Baoshan Sun, Dandan Sun, Wang Jia
BACKGROUND: Sodium/hydrogen exchanger 1 (NHE1), encoded by the SLC9A1 gene (SoLute Carrier family 9A1) in humans, is the main H+ efflux mechanism in maintaining alkaline intracellular pH (pHi ) and Warburg effects in glioma. However, to date, there are no clinical studies exploring pharmacological inhibition of NHE1 protein in cancer treatment. In this study, we investigated NHE1 expression in gliomas and its relationship with glioma clinical outcome. METHODS: The Chinese Glioma Genome Atlas (CGGA) dataset containing transcriptome sequencing data of 325 glioma samples and the Cancer Genome Atlas (TCGA) with 698 glioma mRNAseq data were analyzed in this study...
October 17, 2018: Journal of Experimental & Clinical Cancer Research: CR
Minghui Chang, Xingguo Song, Xinran Geng, Xingwu Wang, Weijun Wang, Thomas C Chen, Li Xie, Xianrang Song
BACKGROUND: Temozolomide-perillyl alcohol conjugate (TMZ-POH), a novel Temozolomide (TMZ) analog developed based on the conjugation of TMZ and perillyl alcohol (POH), displayed strong anticancer potency in multiple cancer types. In this study, we aimed to clarify the relationship between TMZ-POH and autophagy, and explore the underlying mechanisms involved in. METHODS: The proteins involved in autophagy, mitochondrial fission, lysosomal function and membrane traffic were detected by western blots; Autophagosome, mitochondria and lysosome were visualized by transmission electron microscope (TEM) and immunostaining; Apoptosis analysis and fluorescence probe detection were applied by flow cytometry...
October 16, 2018: Journal of Experimental & Clinical Cancer Research: CR
Yawei Shi, Yang Zhao, Yunjian Zhang, NiJiati AiErken, Nan Shao, Runyi Ye, Ying Lin, Shenming Wang
BACKGROUND: Although tamoxifen is a highly effective drug for treating estrogen receptor-positive (ER+ ) breast cancer, nearly all patients with metastasis with initially responsive tumors eventually relapse, and die from acquired drug resistance. Unfortunately, few molecular mediators of tamoxifen resistance have been described. Here, we describe AFF3 (AF4/FMR2 family member 3), which encodes a nuclear protein with transactivation potential that confers tamoxifen resistance and enables estrogen-independent growth...
October 16, 2018: Journal of Experimental & Clinical Cancer Research: CR
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