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Journal of Experimental & Clinical Cancer Research: CR

Serena Veschi, Laura De Lellis, Rosalba Florio, Paola Lanuti, Alberto Massucci, Nicola Tinari, Michele De Tursi, Pierluigi di Sebastiano, Marco Marchisio, Clara Natoli, Alessandro Cama
BACKGROUND: Pancreatic cancer (PC) is the fourth most common cause of cancer death. Combination therapies with classical chemotherapeutic agents improved treatment of advanced PC at the cost of a relevant toxicity, but the 5-year survival rate remains below 5%. Consequently, new therapeutic options for this disease are urgently needed. In this study, we explored the effect of two repurposed drug candidates nelfinavir and nitroxoline, approved for non-anticancer human use, in PC cell lines...
September 21, 2018: Journal of Experimental & Clinical Cancer Research: CR
Baojin Wang, Xia Li, Guannan Zhao, Huan Yan, Peixin Dong, Hidemichi Watari, Michelle Sims, Wei Li, Lawrence M Pfeffer, Yuqi Guo, Junming Yue
BACKGROUND: We previously reported that miR-203 functions as a tumor suppressor in ovarian cancer cells by directly targeting transcription factor Snai2 and inhibiting epithelial to mesenchymal transition (EMT), whereas BIRC5/survivin promotes EMT. In this study, we tested our hypothesis that miR-203 inhibits ovarian tumor metastasis by suppressing EMT through targeting BIRC5, using an orthotopic ovarian cancer mouse model. METHODS: We overexpressed miR-203 in ovarian cancer SKOV3 and OVCAR3 cells using a lentiviral vector and examined cell migration and invasion using transwell plates...
September 21, 2018: Journal of Experimental & Clinical Cancer Research: CR
Pascal Häfliger, Julien Graff, Matthias Rubin, Amandine Stooss, Matthias S Dettmer, Karl-Heinz Altmann, Jürg Gertsch, Roch-Philippe Charles
BACKGROUND: The L-type amino acid transporter 1 (LAT1/SLC7A5) transports essential amino acids across the plasma membrane. While LAT1 is overexpressed in a variety of human neoplasms, its expression and its role in thyroid cancer is currently unknown. Anaplastic thyroid carcinoma (ATC) is a highly aggressive malignancy for which no effective therapy exists. The purpose of this study was to explore whether the inhibition of LAT1 in ATC would affect tumor growth both in vitro and in vivo...
September 21, 2018: Journal of Experimental & Clinical Cancer Research: CR
Cheng-Lung Hsu, Kar-Wai Lui, Lang-Ming Chi, Yung-Chia Kuo, Yin-Kai Chao, Chun-Nan Yeh, Li-Yu Lee, Yenlin Huang, Tung-Liang Lin, Mei-Yuan Huang, Yi-Ru Lai, Yuan-Ming Yeh, Hsien-Chi Fan, An-Chi Lin, Yen-Jung Lu, Chia-Hsun Hsieh, Kai-Ping Chang, Ngan-Ming Tsang, Hung-Ming Wang, Alex Y Chang, Yu-Sun Chang, Hsin-Pai Li
BACKGROUND: Patient-derived xenograft (PDX) tumor model has become a new approach in identifying druggable tumor mutations, screening and evaluating personalized cancer drugs based on the mutated targets. METHODS: We established five nasopharyngeal carcinoma (NPC) PDXs in mouse model. Subsequently, whole-exome sequencing (WES) and genomic mutation analyses were performed to search for genetic alterations for new drug targets. Potential drugs were applied in two NPC PDX mice model to assess their anti-cancer activities...
September 20, 2018: Journal of Experimental & Clinical Cancer Research: CR
Chixiong Huang, Senlin Huang, Hairui Li, Xinzhong Li, Bing Li, Lintao Zhong, Junfeng Wang, Meishen Zou, Xiang He, Hao Zheng, Xiaoyun Si, Wangjun Liao, Yulin Liao, Li Yang, Jianping Bin
BACKGROUND: Multidrug resistance (MDR) is often responsible for the failure of chemotherapy treatment, and current strategies for cancer MDR are not adequately satisfying as to their efficacy and safety. In this study, we sought to determine the anti-MDR effects of ultrasound (US) irradiation and its underlying mechanisms against drug-resistance. METHODS: MDR variant MCF-7/ADR cell lines and endothelial cell lines were used to determine the appropriate ultrasound intensity for in vitro experiments...
September 19, 2018: Journal of Experimental & Clinical Cancer Research: CR
Qing Li, Cong Wang, Yufeng Wang, Liankang Sun, Zhikui Liu, Liang Wang, Tao Song, Yingmin Yao, Qingguang Liu, Kangsheng Tu
BACKGROUND: Cartilage oligomeric matrix protein (COMP) is known to promote fibrosis in skin, lung and liver. Emerging evidence shows that COMP plays critical roles in tumor development, including breast cancer, colon cancer and hepatocellular carcinoma (HCC). Nevertheless, the role of COMP in HCC proliferation and metastasis and its underlying mechanisms remain fully unclear. METHODS: Serum COMP was determined by ELISA. Cell Counting Kit-8 and plate colony formation were performed to evaluate cell proliferation...
September 19, 2018: Journal of Experimental & Clinical Cancer Research: CR
Xi He, Si-Yuan Chen, Zhao Yang, Jie Zhang, Wei Wang, Mei-Yue Liu, Yi Niu, Xiao-Mei Wei, Hong-Min Li, Wan-Ning Hu, Guo-Gui Sun
BACKGROUND: Non-small cell lung cancer (NSCLC) is a leading cause of death worldwide. MicroRNAs (miRNAs) have been indicated as crucial actors in cancer biology. Accumulating evidence suggests that miRNAs can be used as diagnostic and prognostic markers for NSCLC. METHODS: The purpose of this study was to characterize and identify the novel biomarker miR-4317 and its targets in NSCLC. The expression of miR-4317 was analyzed by in situ hybridization (ISH) and quantitative reverse transcription polymerase chain reaction (qRT-PCR)...
September 18, 2018: Journal of Experimental & Clinical Cancer Research: CR
Yang Cheng, Tianlu Hou, Jian Ping, Tianyang Chen, Baobing Yin
BACKGROUND: In this research, we aimed to investigate the biological functions of LIM domain only 3 (LMO3) in hepatocellular carcinoma (HCC) and uncover the underlying molecular mechanism in it. METHODS: HCC tissue microarray (n = 180) was used to analyze the correlation between LMO3 expression and clinicopathological findings. In vitro transwell matrigel invasion assay and annexin V anoikis assay in HCC cells were conducted to investigate LMO3 related biological functions...
September 15, 2018: Journal of Experimental & Clinical Cancer Research: CR
Xian-Yue Ren, Xin Wen, Ying-Qing Li, Jian Zhang, Qing-Mei He, Xiao-Jing Yang, Xin-Ran Tang, Ya-Qin Wang, Pan-Pan Zhang, Xiao-Zhong Chen, Bin Cheng, Jun Ma, Na Liu
BACKGROUND: Increasing evidence recognizes that DNA methylation abnormalities play critical roles in cancer development. Our previous genome-wide methylation profile showed that tumor necrosis factor-alpha-induced protein 8 like 3 (TIPE3) was hypermethylated in nasopharyngeal carcinoma (NPC). However, the relationship between TIPE3 methylation and its mRNA expression, as well as its biological roles in NPC are unknown. METHODS: Bisulfite pyrosequencing and quantitative RT-PCR were performed to quantify the TIPE3 methylation and expression levels...
September 14, 2018: Journal of Experimental & Clinical Cancer Research: CR
Hongmei Zheng, Yuting Zhan, Sile Liu, Junmi Lu, Jiadi Luo, Juan Feng, Songqing Fan
Non-small cell lung cancer (NSCLC) accounts for approximately 85% of lung cancer cases, and it is one of the leading causes of cancer death in both men and women worldwide due to diagnosis in the advanced stage, rapid metastasis, and recurrence. At present, precision molecular targeted therapeutics directed toward NSCLC driven genes has made great progress and significantly improved the overall survival of patients with NSCLC, but can easily lead to acquired drug resistance. New methods are needed to develop real-time monitoring of drug efficacy and drug resistance, such as new molecular markers for more effective early detection and prediction of prognosis...
September 14, 2018: Journal of Experimental & Clinical Cancer Research: CR
Jin Zhang, Hongqing Cai, Lixin Sun, Panpan Zhan, Meng Chen, Feng Zhang, Yuliang Ran, Jinghai Wan
BACKGROUND: Tumor recurrence, the chief reason for poor prognosis of glioma, is largely attributed to glioma stem cells (GSCs) and epithelial-mesenchymal transition (EMT). However, the mechanisms among them remain unknown. Here, we determined whether leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5), known as a stem cell marker for colon cancer and gastric cancer, can serve as a novel GSC marker involved in EMT and a therapeutic target in glioma. METHODS: Stemness properties were examined in FACS-isolated LGR5+ /LGR5- cells...
September 12, 2018: Journal of Experimental & Clinical Cancer Research: CR
Wang Li, Ting Zhang, Lianying Guo, Lin Huang
Phosphatase and tensin homologue (PTEN) triggers a battery of intracellular signaling pathways, especially PI3K/Akt, playing important roles in the pathogenesis of multiple diseases, such as cancer, neurodevelopmental disorders, cardiovascular dysfunction and so on. Therefore PTEN might be a biomarker for various diseases, and targeting the abnormal expression level of PTEN is anticipated to offer novel therapeutic avenues. Recently, noncoding RNAs (ncRNAs) have been reported to regulate protein expression, and it is definite that PTEN expression is controlled by ncRNAs epigenetically or posttranscriptionally as well...
September 10, 2018: Journal of Experimental & Clinical Cancer Research: CR
Jiangtao Yu, Zhiyang Han, Ziquan Sun, Yue Wang, Ming Zheng, Chunfang Song
BACKGROUND: Emerging evidence has shown long noncoding RNAs (lncRNAs) exert important roles in colorectal cancer (CRC) tumorigenesis. However, most lncRNAs involved in this process remain undefined and the underlying molecular mechanisms mediated by lncRNAs are largely unknown. METHODS: An unbiased screening was used to identify novel lncRNAs involved in CRC according to an online-available data dataset. In situ hybridization (ISH) and qRT-PCR was used to detect lncRNA expression patterns...
September 10, 2018: Journal of Experimental & Clinical Cancer Research: CR
Bo Wang, Ping Fan, Jingyuan Zhao, Heyu Wu, Xin Jin, Heshui Wu
BACKGROUND: Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal tumor types worldwide. BET inhibitors display anti-tumor activity in pancreatic cancer, however the cells often develop resistance after a long-term treatment and the underlying molecular basis is not fully understood. METHODS: Drug screening assay in Fructose-1, 6-biphosphatase (FBP1) knockdown or overexpressing pancreatic cancer cells was performed. Tumor cell motility, FBP1 protein and mRNA changes were investigated after BET inhibitors treatment...
September 10, 2018: Journal of Experimental & Clinical Cancer Research: CR
Ruizhe Zhao, Xiaoyu Bei, Boyu Yang, Xiaohai Wang, Chenyi Jiang, Fei Shi, Xingjie Wang, Yiping Zhu, Yifeng Jing, Bangmin Han, Shujie Xia, Qi Jiang
BACKGROUND: Prostate cancer is one of the most common malignancies. Increasing evidence suggested that endothelial cells may contribute to prostate cancer progression and metastasis. Most recently, autophagy has been proposed to plays a significant role in tumorigenesis and metastasis. Also, it is reported that downregulation of androgen receptor (AR) induces autophagy in prostate cancer cells. However, the underlying mechanisms remain unclear. Here, we aim to explore the role and mechanisms of endothelial cell in prostate cancer progression...
September 10, 2018: Journal of Experimental & Clinical Cancer Research: CR
Sisi Cao, Zhuo Wang, Xiujuan Gao, Wenjuan He, Yue Cai, Hui Chen, Rong Xu
BACKGROUND: Accumulating evidence suggests that cancer stem cells (CSCs) play a critical role in tumor initiation, progression and therapy, and recent studies have indicated that Forkhead box C1 (FOXC1) is strongly associated with CSCs. This study investigates the regulatory effects of FOXC1 on CSC-like properties in non-small cell lung cancer (NSCLC). METHODS: We analyzed FOXC1 expression in NSCLC using the Cancer Genome Atlas (TCGA) database on UALCANC and performed survival analyses of NSCLC patients on Human Protein Atlas...
September 6, 2018: Journal of Experimental & Clinical Cancer Research: CR
Simona di Martino, Gabriele De Luca, Ludovica Grassi, Giulia Federici, Romina Alfonsi, Michele Signore, Antonio Addario, Laura De Salvo, Federica Francescangeli, Massimo Sanchez, Valentina Tirelli, Giovanni Muto, Isabella Sperduti, Steno Sentinelli, Manuela Costantini, Luca Pasquini, Michele Milella, Mustapha Haoui, Giuseppe Simone, Michele Gallucci, Ruggero De Maria, Désirée Bonci
BACKGROUND: Clear cell RCC (ccRCC) accounts for approximately 75% of the renal cancer cases. Surgery treatment seems to be the best efficacious approach for the majority of patients. However, a consistent fraction (30%) of cases progress after surgery with curative intent. It is currently largely debated the use of adjuvant therapy for high-risk patients and the clinical and molecular parameters for stratifying beneficiary categories. In addition, the treatment of advanced forms lacks reliable driver biomarkers for the appropriated therapeutic choice...
September 5, 2018: Journal of Experimental & Clinical Cancer Research: CR
Sen-Mao Li, Huan-Lei Wu, Xiao Yu, Kun Tang, Shao-Gang Wang, Zhang-Qun Ye, Jia Hu
BACKGROUND: Previous studies report that miR-1-3p, a member of the microRNA-1 family (miR-1), and functions as a tumor suppressor in several different cancers. However, little is known regarding the biological role and intrinsic regulatory mechanisms of miR-1-3p in prostate cancer (PCa). METHODS: In this study, the expression levels of miR-1-3p were first examined in PCa cell lines and tumor tissues by RT-qPCR and bioinformatics. The in vitro and in vivo functional effect of miR-1-3p was examined further...
September 5, 2018: Journal of Experimental & Clinical Cancer Research: CR
Sheng Zhou, Hongwei Xia, Huanji Xu, Qiulin Tang, Yongzhan Nie, Qi Yong Gong, Feng Bi
BACKGROUND: ERRα, a constitutive transcription factor that regulates energy metabolism, plays an important role in the progression of various tumours. However, its role in cell survival and proliferation and its implication in targeted therapy in colon cancer remains elusive. METHODS: The expression of ERRα in colon cancer tissues and cell lines was detected by using western blotting and immunohistochemistry. A wound healing assay and a transwell assay were performed to examine the migration and invasion of the colon cancer cells...
September 5, 2018: Journal of Experimental & Clinical Cancer Research: CR
Wuhua Zhou, Li Gong, Qinchuan Wu, Chunyang Xing, Bajin Wei, Tianchi Chen, Yuan Zhou, Shengyong Yin, Bin Jiang, Haiyang Xie, Lin Zhou, Shusen Zheng
BACKGROUND: Plant homeodomain finger protein 8 (PHF8) serves an activator of epithelial-mesenchymal transition (EMT) and is implicated in various tumors. However, little is known about PHF8 roles in hepatocellular carcinoma (HCC) and regulating E-cadherin expression. METHODS: PHF8 expression pattern was investigated by informatic analysis and verified by RT-qPCR and immunochemistry in HCC tissues and cell lines. CCK8, xenograft tumor model, transwell assay, and tandem mCherry-GFP-LC3 fusion protein assay were utilized to assess the effects of PHF8 on proliferation, metastasis and autophagy of HCC cells in vitro and in vivo...
September 4, 2018: Journal of Experimental & Clinical Cancer Research: CR
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