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Journal of Experimental & Clinical Cancer Research: CR

Liyan Wang, Xiaotong Bo, Qinghua Zheng, Wenhong Ge, Yanhua Liu, Bin Li
BACKGROUND: Paired box 8 (PAX8) has been documented to be downregulated in gastric cancer. However, its biological function in this malignancy is poorly understood. METHODS: In the present work, we investigated the effects of PAX8 overexpression and knockdown on the aggressive phenotype of gastric cancer cells. We further checked the involvement of forkhead box M1 (FOXM1), a ubiquitously expressed oncogene that can facilitate gastric cancer progression, in the action of PAX8...
July 18, 2018: Journal of Experimental & Clinical Cancer Research: CR
Yubo Tang, Jincheng Pan, Shuai Huang, Xinsheng Peng, Xuenong Zou, Yongxiang Luo, Dong Ren, Xin Zhang, Ronggang Li, Peiheng He, Qingde Wa
BACKGROUND: Bone metastasis is a leading cause of morbidity and mortality in advanced prostate cancer (PCa). Downexpression of miR-133a-3p has been found to contribute to the progression, recurrence and distant metastasis in PCa. However, clinical significance of miR-133a-3p in bone metastasis of PCa, and the biological role of miR-133a-3p and its molecular mechanisms underlying bone metastasis of PCa remain unclear. METHODS: miR-133a-3p expression was evaluated in 245 clinical PCa tissues by real-time PCR...
July 18, 2018: Journal of Experimental & Clinical Cancer Research: CR
Xiupeng Xu, Zhongyuan Bao, Yinlong Liu, Kuan Jiang, Tongle Zhi, Dong Wang, Liang Fan, Ning Liu, Jing Ji
BACKGROUND: Brain invasion by glioblastoma (GBM) determines recurrence and prognosis in patients, which is, in part, attributed to increased mesenchymal transition. Here, we report evidence favoring such a role for the Pre-B-cell leukemia homebox (PBX) family member PBX3. METHODS: Western blot, immunohistochemistry, qRT-PCR and datasets mining were used to determined proteins or genes expression levels. Wound-healing and transwell assays were used to examine the invasive abilities of GBM cells...
July 17, 2018: Journal of Experimental & Clinical Cancer Research: CR
Jia Shen, Tao Zhang, Zheng Cheng, Ni Zhu, Hua Wang, Li Lin, Zexia Wang, Haotian Yi, Meichun Hu
BACKGROUND: Lycorine has been revealed to inhibit the development of many kinds of malignant tumors, including glioblastoma multiforme (GBM). Although compelling evidences demonstrated Lycorine's inhibition on cancers through some peripheral mechanism, in-depth mechanism studies of Lycotine's anti-GBM effects still call for further exploration. Epidermal Growth Factor Receptor (EGFR) gene amplification and mutations are the most common oncogenic events in GBM. Targeting EGFR by small molecular inhibitors is a rational strategy for GBM treatment...
July 17, 2018: Journal of Experimental & Clinical Cancer Research: CR
Feifei Zhang, Kaitao Li, Mingxin Pan, Weidong Li, Juan Wu, Mingyi Li, Liang Zhao, Hui Wang
BACKGROUND: As novel biomarkers for various cancers, microRNAs negatively regulate genes expression via promoting mRNA degradation and suppressing mRNA translation. miR-589 has been reported to be deregulated in several human cancer types. However, its biological role has not been functionally characterized in gastric cancer. Here, we aim to investigate the biological effect of miR-589 on gastric cancer and to reveal the possible mechanism. METHODS: Real-time PCR was performed to evaluate the expression of miR-589 in 34 paired normal and stomach tumor specimens, as well as gastric cell lines...
July 16, 2018: Journal of Experimental & Clinical Cancer Research: CR
Ganlu Deng, Shan Zeng, Yanling Qu, Qingqing Luo, Cao Guo, Ling Yin, Ying Han, Yiyi Li, Changjing Cai, Yaojie Fu, Hong Shen
BACKGROUND: Autophagy is a conserved catabolic process with complicated roles in tumor development. Bone morphogenetic protein 4 (BMP4), a member of the transforming growth factor (TGF-β) family of regulatory proteins, plays a crucial role in human malignancies. However, whether BMP4 contributes to the regulation of autophagy in hepatocellular carcinoma (HCC) progression remains elusive. METHODS: Functional analysis of BMP4 on HCC proliferation and autophagy was performed both in vitro and in vivo in HepG2 and HCCLM3 cells...
July 16, 2018: Journal of Experimental & Clinical Cancer Research: CR
Mingkun Chen, Rongpei Wu, Gang Li, Cundong Liu, Lei Tan, Kanghua Xiao, Yunlin Ye, Zike Qin
BACKGROUND: Uncontrolled proliferation is thought to be the most fundamental characteristic of cancer. Detailed knowledge of cancer cell proliferation mechanisms would not only benefit understanding of cancer progression, but may also provide new clues for developing novel therapeutic strategies. METHODS: In vitro function of MNX1 (Motor neuron and pancreas homeobox 1) in bladder cancer cell was evaluated using MTT assay, colony formation assay, and bromodeoxyuridine incorporation assay...
July 16, 2018: Journal of Experimental & Clinical Cancer Research: CR
Mei Yi, Junjun Li, Shengnan Chen, Jing Cai, Yuanyuan Ban, Qian Peng, Ying Zhou, Zhaoyang Zeng, Shuping Peng, Xiaoling Li, Wei Xiong, Guiyuan Li, Bo Xiang
In the publication of this article [1], there is an error in the Figure caption of Figs. 2, 3 and 4. This has now been included in this correction. The authors declare that these corrections do not change the results or conclusions of this paper.
July 16, 2018: Journal of Experimental & Clinical Cancer Research: CR
Linglong Yin, Youhong Liu, Yuchong Peng, Yongbo Peng, Xiaohui Yu, Yingxue Gao, Bowen Yuan, Qianling Zhu, Tuoyu Cao, Leye He, Zhicheng Gong, Lunquan Sun, Xuegong Fan, Xiong Li
BACKGROUND: The poly ADP ribose polymerase (PARP) inhibitor olaparib has been approved for treating prostate cancer (PCa) with BRCA mutations, and veliparib, another PARP inhibitor, is being tested in clinical trials. However, veliparib only showed a moderate anticancer effect, and combination therapy is required for PCa patients. Histone deacetylase (HDAC) inhibitors have been tested to improve the anticancer efficacy of PARP inhibitors for PCa cells, but the exact mechanisms are still elusive...
July 16, 2018: Journal of Experimental & Clinical Cancer Research: CR
Mao-Hua Cai, Xiao-Gang Xu, Shi-Li Yan, Ze Sun, Yin Ying, Bai-Kui Wang, Yue-Xing Tu
BACKGROUND: Colorectal cancer (CRC) is one of the most prevalent malignancies in the world and developed drug resistance has represented one of the most challenging tasks for management. The current therapeutic regimens may select and enrich cancer stem-like cells (CSCs) resulting in the increased resistance against treatment, metastatic potential and mortality. Regorafenib is a multi-kinase inhibitor, an FDA-approved last-of-line treatment for patients with chemo-refractory metastatic CRC...
July 13, 2018: Journal of Experimental & Clinical Cancer Research: CR
Haige Ye, Aakash Desai, Shengjian Huang, Dayoung Jung, Richard Champlin, Dongfeng Zeng, Fangfang Yan, Krystle Nomie, Jorge Romaguera, Makhdum Ahmed, Michael L Wang
The natural history of mantle cell lymphoma (MCL) is a continuous process with the vicious cycle of remission and recurrence. Because MCL cells are most vulnerable before their exposure to therapeutic agents, front-line therapy could eliminate MCL cells at the first strike, reduce the chance for secondary resistance, and cause long-term remissions. If optimized, it could become an alternative to cure MCL. The key is the intensity of front-line therapy. Both the Nordic 2 and the MD Anderson Cancer Center HCVAD trials, with follow-up times greater than 10 years, achieved long-term survivals exceeding 10 years...
July 13, 2018: Journal of Experimental & Clinical Cancer Research: CR
Yichao Mo, Longguang He, Zeru Lai, Zhiheng Wan, Qinshou Chen, Sibo Pan, Liangfu Li, Dasheng Li, Junwei Huang, Fan Xue, Siyao Che
BACKGROUND: The long non-coding RNAs (lncRNAs) have participated in the promotion of hepatocellular carcinoma (HCC) initiation and progression. Nevertheless, the biological role and underlying mechanism of LINC01287 in HCC has never been reported. METHODS: The TGCA database was used to explore the abnormal expression of lncRNAs in HCC. Real-time PCR and in situ hybridization assays were used to examine the expression of LINC01287 in HCC tissues. The clinicopathological characteristics of HCC patients in relation to LINC01287 expression were then analyzed...
July 13, 2018: Journal of Experimental & Clinical Cancer Research: CR
Wenlu Li, Dandan Zhu, Shuaihua Qin
BACKGROUND: Oral squamous cell carcinoma (OSCC) is one of the most common malignancies and has a poor prognosis. The epithelial-to-mesenchymal transition (EMT) is crucial for increasing the metastasis of OSCC. Recently, studies have indicated that sirtuin7 (SIRT7) is implicated in tumor genesis; however, the potential role of SIRT7 in the EMT and metastasis of OSCC has not been reported. METHODS: We investigated the cellular responses to SIRT7 silencing or overexpression in OSCC cell lines by wound healing assay, migration and invasion assay, western blotting, immunofluorescence and immunohistochemistry...
July 13, 2018: Journal of Experimental & Clinical Cancer Research: CR
Juan Lu, Qi-Hui Liu, Fan Wang, Jia-Jie Tan, Yue-Qin Deng, Xiao-Hong Peng, Xiong Liu, Bao Zhang, Xia Xu, Xiang-Ping Li
BACKGROUND: Exosomes are small vesicles containing a wide range of functional proteins, mRNA and miRNA. Exosomal miRNAs from cancer cells play crucial roles in mediating cell-cell communication and tumor-microenvironment cross talk, specifically in enabling metastasis and promoting angiogenesis. We focused on miR-9 that was identified as a tumor suppressor previously in nasopharyngeal carcinoma (NPC) tumorigenesis. METHODS: Differential centrifugation, transmission electron microscopy and nanoparticle tracking analysis were used to isolate and identify exosomes...
July 13, 2018: Journal of Experimental & Clinical Cancer Research: CR
Jian Ma, Kun Chang, Jingtao Peng, Qing Shi, Hualei Gan, Kun Gao, Kai Feng, Fujiang Xu, Hailiang Zhang, Bo Dai, Yao Zhu, Guohai Shi, Yijun Shen, Yiping Zhu, Xiaojian Qin, Yao Li, Pingzhao Zhang, Dingwei Ye, Chenji Wang
BACKGROUND: Next-generation sequencing of the exome and genome of prostate cancers has identified numerous genetic alterations. SPOP (Speckle-type POZ Protein) is one of the most frequently mutated genes in primary prostate cancer, suggesting that SPOP may be a potential driver of prostate cancer. The aim of this work was to investigate how SPOP mutations contribute to prostate cancer development and progression. METHODS: To identify molecular mediators of the tumor suppressive function of SPOP, we performed a yeast two-hybrid screen in a HeLa cDNA library using the full-length SPOP as bait...
July 11, 2018: Journal of Experimental & Clinical Cancer Research: CR
Katia Rea, Francesca Roggiani, Loris De Cecco, Francesco Raspagliesi, Maria Luisa Carcangiu, Joyce Nair-Menon, Marina Bagnoli, Ileana Bortolomai, Delia Mezzanzanica, Silvana Canevari, Antonis Kourtidis, Panos Z Anastasiadis, Antonella Tomassetti
BACKGROUND: The disruption of E-cadherin-mediated adhesion is considered an important driver of tumor progression. Nevertheless, numerous studies have demonstrated that E-cadherin promotes growth- or invasion-related signaling, contrary to the prevailing notion. During tumor progression, epithelial ovarian cancer (EOC) maintains E-cadherin expression and can positively affect EOC cell growth by contributing to PI3K/AKT activation. In polarized epithelia PLEKHA7, a regulator of the zonula adherens integrity, impinges E-cadherin functionality, but its role in EOCs has been never studied...
July 11, 2018: Journal of Experimental & Clinical Cancer Research: CR
Fan Wu, Pin Gao, Wei Wu, Zaozao Wang, Jie Yang, Jiabo Di, Beihai Jiang, Xiangqian Su
BACKGROUND: Serine/threonine protein kinase 25 (STK25) is critical in regulating whole-body glucose and insulin homeostasis and the accumulation of ectopic lipids. The Warburg effect, also known as aerobic glycolysis, is an essential metabolic characteristic of cancer cells. However, the effects of STK25 on aerobic glycolysis of cancer cells remain unexplored. The aim of this study is to investigate the role of STK25 in colorectal cancer (CRC) and to elucidate the underlying mechanisms...
July 11, 2018: Journal of Experimental & Clinical Cancer Research: CR
Meng Xiao, Jianjun Zhang, Wanjun Chen, Wantao Chen
BACKGROUND: Treatment strategies targeting tumor-associated macrophages (TAMs) have been proposed in cancer areas. The functional alterations of macrophages in the microenvironment during the tumorigenesis of human epithelial cancer remain poorly understood. Here, we explored phenotypic alteration of macrophages during the development of oral squamous cell carcinoma (OSCC). METHODS: Conditioned media (CM) and exosome supernatants were harvested from normal oral epithelium, oral leukoplakia cells and OSCC cells...
July 9, 2018: Journal of Experimental & Clinical Cancer Research: CR
Anais Del Curatolo, Fabiana Conciatori, Ursula Cesta Incani, Chiara Bazzichetto, Italia Falcone, Vincenzo Corbo, Sabrina D'Agosto, Adriana Eramo, Giovanni Sette, Isabella Sperduti, Teresa De Luca, Mirko Marabese, Senji Shirasawa, Ruggero De Maria, Aldo Scarpa, Massimo Broggini, Donatella Del Bufalo, Francesco Cognetti, Michele Milella, Ludovica Ciuffreda
BACKGROUND: Mounting evidence suggests that RAF-mediated MEK activation plays a crucial role in paradox MAPK (re)activation, leading to resistance and therapeutic failure with agents hitting a single step along the MAPK cascade. METHODS: We examined the molecular and functional effects of single and combined BRAF (dabrafenib), pan-RAF (RAF265), MEK (trametinib) and EGFR/HER2 (lapatinib) inhibition, using Western Blot and conservative isobologram analysis to assess functional synergism, and explored genetic determinants of synergistic interactions...
July 9, 2018: Journal of Experimental & Clinical Cancer Research: CR
Ling Liu, Zile Huang, Jingjing Chen, Jiangang Wang, Shuying Wang
BACKGROUND: JS-K is a nitric oxide (NO) donor and could generate intracellularly high levels of NO. The study explores PP2A as a tumor suppressor is a major determinant mediating JS-K-caused apoptosis in human hepatocellular carcinoma (HCC) cells. METHODS: The human HCC cell lines (PLC5, Huh-7, Bel-7402, SMMC-7721 and HepG2) were used to assess effects of JS-K on cell viability, apoptosis induction and PP2A activation. Effects of JS-K on cell morphology, mitochondrial membrane potential, apoptosis and NO levels were determined in HCC cells expressing PP2A...
July 9, 2018: Journal of Experimental & Clinical Cancer Research: CR
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