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Advances in Biochemical Engineering/biotechnology

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https://www.readbyqxmd.com/read/28795201/downstream-processing-technologies-capturing-and-final-purification-opportunities-for-innovation-change-and-improvement-a-review-of-downstream-processing-developments-in-protein-purification
#1
Nripen Singh, Sibylle Herzer
Increased pressure on upstream processes to maximize productivity has been crowned with great success, although at the cost of shifting the bottleneck to purification. As drivers were economical, focus is on now on debottlenecking downstream processes as the main drivers of high manufacturing cost. Devising a holistically efficient and economical process remains a key challenge. Traditional and emerging protein purification strategies with particular emphasis on methodologies implemented for the production of recombinant proteins of biopharmaceutical importance are reviewed...
August 10, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28776065/trends-in-process-analytical-technology-present-state-in-bioprocessing
#2
Marco Jenzsch, Christian Bell, Stefan Buziol, Felix Kepert, Harald Wegele, Christian Hakemeyer
Process analytical technology (PAT), the regulatory initiative for incorporating quality in pharmaceutical manufacturing, is an area of intense research and interest. If PAT is effectively applied to bioprocesses, this can increase process understanding and control, and mitigate the risk from substandard drug products to both manufacturer and patient. To optimize the benefits of PAT, the entire PAT framework must be considered and each elements of PAT must be carefully selected, including sensor and analytical technology, data analysis techniques, control strategies and algorithms, and process optimization routines...
August 4, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28776064/manufacturing-of-proteins-and-antibodies-chapter-downstream-processing-technologies-harvest-operations
#3
Richard Turner, Adrian Joseph, Nigel Titchener-Hooker, Jean Bender
Cell harvesting is the separation or retention of cells and cellular debris from the supernatant containing the target molecule Selection of harvest method strongly depends on the type of cells, mode of bioreactor operation, process scale, and characteristics of the product and cell culture fluid. Most traditional harvesting methods use some form of filtration, centrifugation, or a combination of both for cell separation and/or retention. Filtration methods include normal flow depth filtration and tangential flow microfiltration...
August 4, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28776063/challenges-and-perspectives-in-nucleic-acid-enzyme-engineering
#4
Darko Balke, Robert Hieronymus, Sabine Müller
Engineering of nucleic acids has been a goal in research for many years. Since the discovery of catalytic nucleic acids (ribozymes and DNAzymes), this field has attracted even more attention. One reason for the increased interest is that a large number of ribozymes have been engineered that catalyze a broad range of reactions of relevance to the origin of life. Another reason is that the structures of ribozymes or DNAzymes have been modulated such that activity is dependent on allosteric regulation by an external cofactor...
August 4, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28707104/microbial-hydrocarbon-formation-from-biomass
#5
Adrie J J Straathof, Maria C Cuellar
Fossil carbon sources mainly contain hydrocarbons, and these are used on a huge scale as fuel and chemicals. Producing hydrocarbons from biomass instead is receiving increased attention. Achievable yields are modest because oxygen atoms need to be removed from biomass, keeping only the lighter carbon and hydrogen atoms. Microorganisms can perform the required conversions, potentially with high selectivity, using metabolic pathways that often end with decarboxylation. Metabolic and protein engineering are used successfully to achieve hydrocarbon production levels that are relevant in a biorefinery context...
July 14, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28642967/strategies-for-characterization-of-enzymatic-nucleic-acids
#6
Fatemeh Javadi-Zarnaghi, Claudia Höbartner
Practical application of enzymatic nucleic acids has received more attention in recent years. Understanding the mechanism of catalysis and availability of information on potentials and limitations of these enzymes expands their application scope. A general approach for characterization of functional macromolecules including enzymatic nucleic acids is to perturb a specific set of condition and to follow the perturbation effect by biophysical and biochemical methods. This chapter reviews several perturbation strategies for functional nucleic acids, including deletion, mutation, and modifications of backbone and nucleobases, and consequent kinetic analysis, spectroscopic investigations, and probing assays...
June 23, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28631029/syngas-biorefinery-and-syngas-utilization
#7
Sashini De Tissera, Michael Köpke, Sean D Simpson, Christopher Humphreys, Nigel P Minton, Peter Dürre
Autotrophic acetogenic bacteria are able to capture carbon (CO or CO2) through gas fermentation, allowing them to grow on a spectrum of waste gases from industry (e.g., steel manufacture and oil refining, coal, and natural gas) and to produce ethanol. They can also consume syn(thesis) gas (CO and H2) made from the gasification of renewable/sustainable resources, such as biomass and domestic/agricultural waste. Acetogenic gas fermentation can, therefore, produce ethanol in any geographic region without competing for food or land...
June 20, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28585079/new-mammalian-expression-systems
#8
Jie Zhu, Diane Hatton
There are an increasing number of recombinant antibodies and proteins in preclinical and clinical development for therapeutic applications. Mammalian expression systems are key to enabling the production of these molecules, and Chinese hamster ovary (CHO) cell platforms continue to be central to delivery of the stable cell lines required for large-scale production. Increasing pressure on timelines and efficiency, further innovation of molecular formats and the shift to new production systems are driving developments of these CHO cell line platforms...
June 6, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28567487/synthetic-biology-of-polyhydroxyalkanoates-pha
#9
De-Chuan Meng, Guo-Qiang Chen
Microbial polyhydroxyalkanoates (PHA) are a family of biodegradable and biocompatible polyesters which have been extensively studied using synthetic biology and metabolic engineering methods for improving production and for widening its diversity. Synthetic biology has allowed PHA to become composition controllable random copolymers, homopolymers, and block copolymers. Recent developments showed that it is possible to establish a microbial platform for producing not only random copolymers with controllable monomers and their ratios but also structurally defined homopolymers and block copolymers...
June 1, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28567486/xenobiology-state-of-the-art-ethics-and-philosophy-of-new-to-nature-organisms
#10
Markus Schmidt, Lei Pei, Nediljko Budisa
The basic chemical constitution of all living organisms in the context of carbon-based chemistry consists of a limited number of small molecules and polymers. Until the twenty-first century, biology was mainly an analytical science and has now reached a point where it merges with engineering science, paving the way for synthetic biology. One of the objectives of synthetic biology is to try to change the chemical compositions of living cells, that is, to create an artificial biological diversity, which in turn fosters a new sub-field of synthetic biology, xenobiology...
June 1, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28540404/biotechnological-and-biochemical-utilization-of-lignin
#11
Dominik Rais, Susanne Zibek
This chapter provides an overview of the biosynthesis and structure of lignin. Moreover, examples of the commercial use of lignin and its promising future implementation are briefly described. Many applications are still hampered by the properties of technical lignins. Thus, the major challenge is the conversion of lignins into suitable building blocks or aromatics in order to open up new avenues for the usage of this renewable raw material. This chapter focuses on details about natural lignin degradation by fungi and bacteria, which harbor potential tools for lignin degradation and modification, which might help to develop eco-efficient processes for lignin utilization...
May 25, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28534167/platforms-for-manufacturing-allogeneic-autologous-and-ipsc-cell-therapy-products-an-industry-perspective
#12
Eytan Abraham, Behnam Baghbaderani Ahmadian, Kathryn Holderness, Yonatan Levinson, Erika McAfee
As cell therapy processes mature from benchtop research protocols to industrial processes capable of manufacturing market-relevant numbers of doses, new cell manufacturing platforms are required. Here we give an overview of the platforms and technologies currently available to manufacture allogeneic cell products, such as mesenchymal stem cells (MSCs) and induced pluripotent stem cells (iPSCs), and technologies for mass production of autologous cell therapies via scale-out. These technologies include bioreactors, microcarriers, cell separation and cryopreservation equipment, molecular biology tools for iPSC generation, and single-use controlled-environment systems for autologous cell production...
May 23, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28474157/bioanalytical-application-of-peroxidase-mimicking-dnazymes-status-and-challenges
#13
J Kosman, B Juskowiak
DNAzymes with peroxidase-mimicking activity are a new class of catalytically active DNA molecules. This system is formed as a complex of hemin and a G-quadruplex structure created by oligonucleotides rich in guanine. Considering catalytic activity, this DNAzyme mimics horseradish peroxidase, the enzyme most commonly used for signal generation in bioassays. Because DNAzymes exhibit many advantages over protein enzymes (thermal stability, easy and cheap synthesis and purification) they can successfully replace HRP in bioanalytical applications...
May 5, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28432390/biomass-resources-agriculture
#14
Ingeborg N Kluts, Marnix L J Brinkman, Sierk A de Jong, H Martin Junginger
Bioenergy is the single largest source of renewable energy in the European Union (EU-28); of this, 14% was produced from agricultural feedstocks in 2012. This chapter provides an overview of the current use (for bioenergy) and future potential of agricultural feedstocks for (amongst others) biorefinery purposes in the European Union. The main application of these feedstocks is currently the production of biofuels for road transport. Biodiesel makes up 80% of the European biofuel production, mainly from rapeseed oil, and the remaining part is bioethanol from wheat and sugar beet...
April 22, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28424826/synergizing-13-c-metabolic-flux-analysis-and-metabolic-engineering-for-biochemical-production
#15
Weihua Guo, Jiayuan Sheng, Xueyang Feng
Metabolic engineering of industrial microorganisms to produce chemicals, fuels, and drugs has attracted increasing interest as it provides an environment-friendly and renewable route that does not depend on depleting petroleum sources. However, the microbial metabolism is so complex that metabolic engineering efforts often have difficulty in achieving a satisfactory yield, titer, or productivity of the target chemical. To overcome this challenge, (13)C Metabolic Flux Analysis ((13)C-MFA) has been developed to investigate rigorously the cell metabolism and quantify the carbon flux distribution in central metabolic pathways...
April 20, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28391363/anaerobic-digestion
#16
Jan Liebetrau, Heike Sträuber, Jörg Kretzschmar, Velina Denysenko, Michael Nelles
The term anaerobic digestion usually refers to the microbial conversion of organic material to biogas, which mainly consists of methane and carbon dioxide. The technical application of the naturally-occurring process is used to provide a renewable energy carrier and - as the substrate is often waste material - to reduce the organic matter content of the substrate prior to disposal.Applications can be found in sewage sludge treatment, the treatment of industrial and municipal solid wastes and wastewaters (including landfill gas utilization), and the conversion of agricultural residues and energy crops...
April 9, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28382477/rna-g-quadruplexes-as-key-motifs-of-the-transcriptome
#17
Samuel Rouleau, Rachel Jodoin, Jean-Michel Garant, Jean-Pierre Perreault
G-Quadruplexes are non-canonical secondary structures that can be adopted under physiological conditions by guanine-rich DNA and RNA molecules. They have been reported to occur, and to perform multiple biological functions, in the genomes and transcriptomes of many species, including humans. This chapter focuses specifically on RNA G-quadruplexes and reviews the most recent discoveries in the field, as well as addresses the upcoming challenges researchers studying these structures face.
April 6, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28374047/bioplastics
#18
Hans-Josef Endres
The number of newly developed bioplastics has increased sharply in recent years and innovative polymer materials are increasingly present on the plastics market. Bioplastics are not, however, a completely new kind of material, but rather a rediscovered class of materials within the familiar group of materials known as plastics. Therefore, existing knowledge from the plastics sector can and should be transferred to bioplastics in order to further increase their performance, material diversity and market penetration...
April 4, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28331960/synthesis-gas-biorefinery
#19
N Dahmen, E Henrich, T Henrich
Synthesis gas or syngas is an intermediate, which can be produced by gasification from a variety of carbonaceous feedstocks including biomass. Carbon monoxide and hydrogen, the main constituents of syngas, can be subjected to a broad range of chemical and microbial synthesis processes, leading to gaseous and liquid hydrocarbon fuels as well as to platform and fine chemicals. Gasification of solid biomass differs from coal gasification by chemical composition, heating value, ash behavior, and other technical and biomass related issues...
March 23, 2017: Advances in Biochemical Engineering/biotechnology
https://www.readbyqxmd.com/read/28303295/sugarcane-biorefinery
#20
Sílvio Vaz
Concepts such as biorefinery and green chemistry focus on the usage of biomass, as with the oil value chain. However, it can cause less negative impact on the environment. A biorefinery based on sugarcane (Saccharum spp.) as feedstock is an example, because it can integrate into the same physical space, of processes for obtaining biofuels (ethanol), chemicals (from sugars or ethanol), electricity, and heat.The use of sugarcane as feedstock for biorefineries is dictated by its potential to supply sugars, ethanol, natural polymers or macromolecules, organic matter, and other compounds and materials...
March 17, 2017: Advances in Biochemical Engineering/biotechnology
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